Objective: To determine the encephalic region correlated with epilepsy by manganese-enhanced MRI (MEMRI) and determine the correlation of epilepsy with calcium overloading. Methods: The cats were divided into two groups.The first group underwent EEG examination and ethological observation. The second group underwent MEMRI measurement. Signal enhanced encephalic regions were sectioned. Results: The achievement ratio of convulsive cats intramusclelarly injected with PTZ was 80﹪. MEMRI showed diffuse signal enhancement in the cerebral cortex of the cats with generalized tonic-clonic convulsive seizures compared with control animals. The enhancement rate of frontal-parietal-occipital lobe was 34.6﹪ and 22.9﹪ in temporal lobe compared with the control groups. Signal enhancement on frontal-parietal lobe persisted for 24 h after epileptic seizures were induced. The neurons of enhanced encephalic regions showed obvious degeneration and necrosis. Conclusion: Seizures can be induced in cats by intramuscular injection of PTZ (55 mg/kg). Frontal-parietal lobe is the correlated encephalic regions of epilepsy. MEMRI plays an important role in localizing and revealing pathogenesis of epileptic seizures.