Objective: To establish a rapid and precise continuous flow-injection chemiluminescence (CL) method to determine ribavirin. Methods: The ribavirin could restrain strongly CL reaction of luminol in sodium hydroxide (NaOH) solution with potassium chlorate(KClO3). The different experimental parameters affecting the CL intensity were studied carefully. Results:Under optimum conditions(The concentrations of luminol,KClO3, and NaOH were at 0.1 mmol/L,0.5 μmol/L,and 0.2 mol/L, respectively), the linear range of the working curves was 0.01-7.00 μg/ml with a detection limit of 0.004 μg/ml. Conclusion: The method is simple, rapid and sensitive, and successfully applied to the determination of ribavirin in pharmaceutical preparations and biological fluids.