Cloning and sequence analysis of β-actin gene from Aedes albopictus (Diptera: Culicidae)
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This work was supported by the National Natural Science Foundation of China(No.30628022 and No.30671827)

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    Abstract:

    Objective: To obtain the complete β-actin gene from Aedes albopictus. Methods: Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D. melanogaster. By RT-PCR, the product was amplified, purified, cloned into the pGT vector and sequenced. The β-actin sequence was aligned and phylogenetically analyzed by the BLAST program and the CLUSTAL W program. Results: A sequence of 1132 bp including an open reading frame of 1131 bp was obtained(GenBank DQ657949). The deduced protein had 376 amino acids. Aligned to SWISS-PROT, it exhibited a high level of identity with β-actins from Anopheles, Drosophila and Culex at the amino acid sequence level. Phylogenetic analysis indicated that Ae. albopictus β-actin was much more homologous with invertebrate β-actin than with vertebrate β-actin. Conclusion: The gene may be used as the internal control in the experiments of Ae. albopictus.

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Weijie Wang, Xiaobang Hu, Donghui Zhang, Jianhua Jiao, Yan Sun, Lei Ma, Changliang Zhu.[J].南京医科大学学报(自然科学版英文版),2007,(2):63-67.

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  • Received:January 04,2007
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