Objective： To observe the effects of apigenin on cell proliferation of human pancreatic carcinoma cell line BxPC-3 in vitro. Methods：The inhibitive effects of apigenin at different concentrations（0 -滋mol／L, 100 -滋mol／L, 200 -滋mol／L, and 400 -滋mol／L） on human pancreatic carcinoma cell line BxPC-3 were detected by MTT assays, transmission electron microscope，agarose gel electrophoresis and flow cytometry. The immunohistochemistry was used to detect the expression of Bcl-2 and Bax gene. Results：Apigenin at different concentrations could inhibit the proliferation of human pancreatic carcinoma cell lines BxPC-3, and the inhibitive effect was dose-dependent. The cell cycle of pancreatic carcinoma cells was arrested at G2／M phase. The results of immunohistochemistry showed that the density of apigenin increased, and the expression of Bcl-2 gene was reduced gradually. At the same time the expression of Bax gene was enhanced. Conclusion：Apigenin could inhibit the proliferation of human pancreatic carcinoma cell lines BxPC-3 in vitro. The effect of apoptosis was accompanied with the expression of Bcl-2 decrease and Bax increase.