Abstract:Objective To investigate the characteristics of acoustic parameters and spectrograph of varying laryngeal diseases and discuss the value of acoustic analysis technique by computer in the quantitative and objective evaluation of vocal function.Methods The voice signals of sustained vowel "a" were measured with a computer (Dr.Speech software) in 78 healthy adults and 98 patients with varying laryngeal diseases.The acousic parameters (jitter,shimmer and NNE) and spectrographic characteristics (harmonic waves,formants and noise) were analyzed.The resubs obtained by acoustic technique were compared with those of perceptual evaluation.Results The acoustic parameters were significantly increased in all patients with laryngeal diseases.The mean parameters were increased 0.5～1.5 times in vocal inflammation and 2.0～4.5 times in vocal paralysis or laryngeal carcinoma as much as the control group.The spectrogarph in laryngeal diseases showed irregularity,breakage or lack of harmonic waves and formants,and increase of noise.A three-point scale was used to rate the pathologic spectrograph and coincided with the degrees of hoarseness judged by perceptual analysis.Conclusion The acoustic parameters and spectrograph can be used as objective standards for evaluaing voice function quantitatively.The acoustic analysis by computer technique is of value in the diagnosis and treatment of laryngeal diseases.

Abstract:Obiective To evaluate the value of DNA ploidy patterns and proliferous activity in tumors diagnosis.Methods Total 32 cases of human breast neoplasms were examined by Flow Cytometry (FCM) to get the parameters of DNA index (DI),proliferating Index(PI),S-phase fraction and ploidy patterns.Results The results showed that acuoploidy rate of breast cancer cases was 70%.However,there were no multiploidy patterns appeared in benign breast tumors.DI,PI and S-phase fraction of breast cancer was higher than benign breast neoplasms.DI of Ⅲ grade breast cancer was higher than Ⅱ grade.Conclusion Aeuploid and high proliferous activity could be used for identification of benign and malignant breast diseases.

Abstract:Objective To investigate the toxic damage Jn thalamic tissue mused by thrombin and whether can be reversed.Methods At different time following thrombin was infused into ventricule,the permeability and the destroyed volume of blood brain barrier (BBB),water content and ion content were measured.The animals,which saline infusion and which hirudin infusion six hours later following thrombin injection,as the control groups.Results Six hours later,BBB disruption,brain edema,Na、Ca concentrations increase and K concentration decrease were induced by thrombin.After hirudin infusion,BBB function was rehabilitated and the changes in brain water and brain ion were inhibited.Conclusion Damage to the thalamic tissue might be caused by thrombin and reversed byhirudin.