2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨神经生长导向因子Slit在鸡胚脊髓中的表达模式对发育和再生神经轴突的导向作用.方法:制备地高辛标记的RNA探针,应用原位杂交技术检测Slit基因mRNA在鸡胚不同发育期脊髓中的表达情况.结果:Slit基因mRNA的表达在中线结构区呈现优势.结论:神经生长导向因子Slit在鸡胚脊髓发育期轴突投射和神经通路形成中起重要作用.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:应用噬菌体展示12肽库筛选低氧诱导因子-1α(HIF-1α)相关肽.方法:以抗HIF-1α单克隆抗体(HIF-1αmAb)为配基,采用亲和免疫法从随机12肽库中筛选与抗体特异结合的噬菌体.在筛选过程中,逐轮提高HIF-1α mAb的稀释度并增强洗脱强度.3轮筛选后,随机挑取10个克隆测序.通过膜斑点印迹(Dot-ELISA)进行特异性结合鉴定.结果:经过3轮筛选后,特异性结合的噬菌体得到有效的富集,并且Dot-EL[SA反应阳性逐轮增强.测序后发现有4个克隆的序列完全相同,其12肽氨基酸序列为GPHHYWYHLRLP.结论:噬菌体展示肽库技术可以用于筛选HIF-1相关肽,并为后续的活性鉴定打下基础.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:建立一种多器官功能障碍综合征(MODS)合并急性肾衰竭(ARF)的动物模型.方法:12只健康杂交家猪随机分成实验组(E组)和假手术对照组(C组).E组行盲肠结扎加穿孔(CLP),并加做双侧输尿管结扎术(UL)制作成MODS合并ARF的动物模型,C组行盲肠和双侧输尿管探查术.两组动物均予24 h静脉输液,观察6天中动物的生命体征及主要器官功能改变.结果:E组动物在CLP+UL后4 h直肠温度升高;术后72 h血清肌酐(Cr)、尿素氮(BUN)、K+、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平分别从术前的(91.40±10.85)μmol/L、(4.08±0.93)mmol/L、(3.81±0.57)mmol/L、(62.40±8.20)U/L、(53.00±14.85)U/L升高至(995.25±125.28)μmol/L、(48.80±1.41)mmol/L、(7.37±0.66)mmol/L、(115.75±33.42)U/L、(278.00±96.20)U/L,动脉血二氧化碳分压(PaCO2)从术前的(37.68±3.10)mmHg(1mmHg=0.133 kPa)升高至(51.98±4.51)mmHg(P均＜0.01);pH值分别从术前的7.38±0.04下降至7.25±0.05(P＜0.05);平均动脉压(MAP)从术后0 h的(160.20±18.58)mmHg降至术后72 h的(105.75±13.96)mmHg(P＜0.01),6天内动物全部死亡,平均生存时间(74.96±23.00)h.C组全部存活,且上述指标基本正常.结论:盲肠结扎加穿孔造成严重腹腔感染致明显的多器官功能障碍,加输尿管结扎术造成梗阻少尿型ARF,证明按本法制作MODS合并ARF模型成功、稳定.
李倩 , 刘超 , 袁庆新 , 段宇 , 覃又文 , 刘翠萍 , 武晓泓
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:初步探讨血清钠/碘转运体自身抗体(NIS-Ab)与自身免疫性甲状腺疾病的关系.方法:选择门诊初发的自身免疫性甲状腺疾病患者54例,分为格雷夫斯甲状腺功能亢进(GD)和桥本甲状腺功能减退(HT)两组,采用酶联免疫吸附法检测血清中NIS-Ab水平.结果:GD组患者血清中NIS-Ab含量明显高于正常对照组和HT组;HT组与正常对照组比较血清NIS-Ab量差异无显著性.NIS-Ab在GD和HT患者中的阳性率分别为32%和20%,NIS-Ab水平与促甲状腺激素受体抗体(TRAb)无统计学相关关系.结论:NIS-Ab与自身免疫性甲状腺疾病密切关联,可能在GD和HT的发病机制中扮演重要角色.
杨晓梅 , 姚堃 , 朱建中 , 丁传林 , 窦洁 , 冯东举 , 周锋 , 仇镇宁
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:制备抗人类疱疹病毒7型(HHV-7)单克隆抗体,应用于临床与科研.方法:用纯化的HHV-7南京株YY5免疫Balb/c小鼠,取其脾细胞与骨髓瘤细胞SP2/0融合,经间接ELISA法筛选.间接免疫荧光、免疫印迹试验等方法鉴定单抗.结果:获得了3株分泌抗(HHV-7)单克隆抗体的杂交瘤细胞系,分别命名为6H9、6C11、4D5.单克隆抗体亚类鉴定表明:6H9为IgG1类,6C11、4D5为IgM类.用间接ELISA方法检测单抗腹水滴度分别为:1:6.40×104,1:1.28×105,1:1.00×103.免疫印迹试验结果表明:6C11能与分子质量约70 ku大小的病毒蛋白结合.结论:成功制备并鉴定3株抗HHV-7单克隆抗体,为进一步研究HHV-7的特性及为临床快速诊断提供可能.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:建立大鼠背侧纹状体长时程突触抑制(LTD)的诱导方法,并观察60 mmol/L酒精对大鼠背侧纹状体LTD诱导的影响,初步探讨酒精的作用机制.方法:制备纹状体冠状切面脑片,双极钨丝电极刺激皮层-纹状体传入通路,在大鼠背侧纹状体部位记录诱发的群峰电位(population spike,PS).预先灌流60 mmol/L酒精及N-甲基-D-天冬氨酸(NMDA)受体阻断剂MK-801,再给予强直刺激.结果:对照组给予强直刺激后诱导出明显的LTD,PS幅值减小到原先的20%左右,并持续60 min以上;灌流酒精10 min再给予强直刺激,PS幅值出现先减小而后增加的变化,60 min时已经恢复到强直刺激前的80%;灌流MK-801后给予强直刺激阻断LTD的诱导.结论:强直刺激皮层-纹状体兴奋性传入通路可在背侧纹状体诱导明显的LTD,灌流酒精不影响LTD的产生,然而会影响LTD的保持,NMDA受体参与纹状体LTD的诱导.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:研究帕米膦酸钠对聚乙烯微粒刺激破骨细胞功能的影响,间接探讨帕米膦酸钠防治人工关节无菌性松动的可能性.方法:分离新生新西兰兔长骨中的破骨细胞,与骨片共同培养2、4、6天,试验分3组:对照组、109/ml聚乙烯微粒组、109/ml聚乙烯微粒和10μg/ml帕米膦酸钠共培养组,观察帕米膦酸钠对破骨细胞骨吸收功能的作用.结果:聚乙烯微粒与破骨细胞共同培养6天可以增强其骨吸收功能,帕米膦酸钠可以抑制聚乙烯微粒对破骨细胞的作用.结论:帕米膦酸钠可以抑制聚乙烯微粒对破骨细胞的刺激作用,可用来防治人工关节无菌性松动.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨雾化吸入氯胺酮对气道反应性及肺和小气道病理改变的影响.方法:用整体引喘法分别测定雾化吸入1%～5%氯胺酮5 min后和3%氯胺酮雾化吸入3、5、10、15、30 min后对豚鼠气道反应性的变化.每天豚鼠雾化吸入5%氯胺酮2 h,连续6天后观察肺部的病理变化.测定雾化吸入3%或5%氯胺酮30 min后小鼠的痛阈改变.结果:2%～5%氯胺酮雾化吸入5 min后有明显的气道舒张作用(P＜0.05).3%氯胺酮雾化吸入5 min以上均有舒张气道的效应(P＜0.05),且各时点之间无明显区别(P＞0.05).3%和5%氯胺酮雾化吸入对小鼠的痛阈无影响(P＞0.05).连续6天雾化吸入5%氯胺酮后未见气道与肺明显的病理变化.结论:3%～5%氯胺酮雾化吸入5 min以上有良好的平喘作用.氯胺酮雾化吸入对外周痛阈不产生明显影响.氯胺酮雾化吸入对肺和小气道不产生病理影响.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:观察不同麻醉方法对开胸手术病人红细胞葡萄糖-6磷酸脱氢酶(G-6PD)、磷酸果糖激酶(PFK)活性、血浆脂质过氧化物(MDA)及谷胱甘肽(GSH)的影响.方法:28例胸科手术病人随机分为A、B两组,A组单纯吸入1.5～2.0最低肺泡吸入有效浓度(MAC)的异氟醚全麻,B组为1.0 MAC异氟醚复合连续硬膜外麻醉.于麻醉前、手术60 min、术后1 h、术后第1天和术后第2天分别取静脉血,测定红细胞G-6PD、PFK活性及血浆MDA、GSH浓度.结果:与麻醉前值相比A组病人术后第1天红细胞G-6PD活性明显上升,PFK活性显著下降(P＜0.05);同时血浆MDA浓度显著上升,GSH浓度显著下降(P＜0.05).B组上述指标变化程度均显著低于A组(P＜0.05).结论:开胸手术后红细胞糖代谢向抗氧化应激方向重新调定,异氟醚全麻复合硬膜外阻滞可减缓此变化.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:为研究抗小肠移植排斥治疗提供良好的动物实验模型.方法:选用大鼠进行同种异体异位节段性小肠移植.结果:共施行96只移植手术,模型稳定后的56只小肠移植5天成活率为85.71%.结论:本模型制作成功率较高,适合小肠移植排斥研究.
钱晓峰 , 王学浩 , 张峰 , 李相成 , 王科 , 成峰 , 刘军
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:建立稳定的大鼠减体积肝移植模型,并初步观察移植后肝脏再生的情况.方法:雄性SD大鼠,采用改良"二袖套法"建立大鼠减体积肝移植模型,无肝期(17.0±3.5)min.分别称取供、受体肝脏重量及供肝重量.术后1、4、7天处死大鼠,称取肝脏重量并取肝组织行组织学检查.结果:术后1周存活率85%.术后7天时受体肝脏重量已恢复至术前水平.组织学检查术后4、7天的肝组织可见二倍体及多倍体的肝细胞.结论:通过技术改进,提高了该模型建立的稳定性.大鼠减体积肝移植后供肝仍具有较强的再生能力.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨残肝再生和肝细胞生长因子(HGF)、转化生长因子α(TGF-α)和β(TGF-β)的表达.方法:30只SD大鼠随机分为2组:①组1为大部(68%)肝切除组,15只;②组2为大部(68%)肝叶切除加部分(50%)胰腺切除加近端十二指肠切除组,15只.每组3只大鼠,于术后12、24、48、72和168 h分别处死并采集肝脏样本,计算肝再生率.切除的肝脏组织用RT-PCR技术检测HGF、TGF-α及TGF-β在不同时间点的表达,并与免疫组化法检测的肝细胞增殖细胞核抗原(PCNA)进行对比研究.结果:组1残肝再生率逐步增加,至术后第7天达最高水平;PCNA峰值在术后24 h,随后逐步下降.HGF和TGF-α的表达在术后12 h逐渐增强,24 h达到高峰,随后逐渐下降,168 h时达最低水平,而TGF-β的表达在48 h时达高峰,然后下降.与组1相比,组2可显著减低残肝再生及PCNA峰值,并可显著减少肝细胞HGF、TGF-α及TGF-β的表达.结论:肝胰十二指肠联合切除可减低残肝再生反应,除了肝细胞自身分泌的HGF及TGF-α等细胞因子外,起源于胰腺和十二指肠的其他细胞因子亦可对肝细胞的增殖起调节作用.
杨荣 , 孔祥清 , 盛燕辉 , 曹克将 , 许迪 , 陈明龙
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨成人继发孔房间隔缺损(ASDⅡ)的Amplatzer间隔封闭器(ASO)介入治疗的疗效和安全性.方法:对接受ASO治疗的49例成人ASDⅡ患者的疗效、安全性进行研究.结果:全组患者技术成功率为95.6%(47/49例);ASDⅡ平均大小(28.2±5.3)mm,ASO平均大小(31.1±6.5)mm;术前平均肺动脉收缩压(39.3±13.4)mmHg(1 mmHg=0.133 kPa),肺动脉血流量/主动脉血流量(Qp/Qs)平均为2.4±1.1,ASD的成功封闭率即刻为91.5%(43/47例),术后24 h为100%;手术并发症发生率4.3%(2/47例);平均随访(11.7±3.6)个月,无ASO移位或破损.结论:成人ASDⅡ的ASO治疗疗效和安全性均好,在有适应证的范围内可替代外科手术.
徐娟 , 王慧娟 , 姚婷 , 王书奎 , 王玲 , 柯瑶 , 季晓辉
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:检测系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中不同细胞亚群发生凋亡的情况,并初步分析其在SLE发病机制中的意义.方法:取28例SLE患者和性别年龄与之相匹配的20个正常对照者.采用淋巴细胞亚群标志、An-nexin-V及碘化丙啶三色荧光标记、流式细胞术检测细胞的早期凋亡.结果:SLE患者体内CD3+T细胞、CD4+T细胞和CD8+T细胞的凋亡百分率均显著高于正常对照组(P＜0.05).同时,SLE患者T淋巴细胞的死亡百分率也高于正常人.经激素治疗一定时间后,这些细胞亚群的凋亡率会进一步升高(P＜0.01),但CD4/CD8比值恢复至接近正常人.SLE患者体内CD19+B淋巴细胞的凋亡或死亡百分率和正常对照组相比均无明显差别,激素治疗对B细胞凋亡和死亡的影响亦不显著.结论:在SLE患者体内主要是T淋巴细胞凋亡增加,但SLE患者淋巴细胞数目减少不仅仅是因为细胞凋亡所致,死亡细胞数目明显升高也是其中一个重要原因.激素治疗可诱导SLE患者T淋巴细胞进一步发生凋亡,并使T细胞亚群比例有所改善.
林泓怡 , 李跃华 , 郭琳 , 阙琳利 , 李菁 , 季晓辉
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:提取并纯化正常人脑脊髓的髓磷脂碱性蛋白(MBP),鉴定其活性,并用其诱导实验性自身免疫性脑脊髓炎(EAE)大鼠模型.方法:以人脑脊髓为原料,通过去脂、酸溶解、CM-52柱层析等步骤提取人MBP,鉴定其纯度后在Wistar大鼠诱导EAE.结果:所提取的MBP具有纯度高、免疫活性好的特点,与抗MBP抗体能特异性结合,并可成功地诱导出EAE动物模型,病理学证实发病大鼠的脑脊髓具有典型的脱髓鞘改变和胶质结节形成.结论:用人MBP在不加百日咳杆菌的条件下,同样可以诱导出EAE,这种动物模型体内的病理学改变与人类的多发性硬化症(MS)更具有相似性,适用于对MS的研究.
鲍华英 , 陈荣华 , 潘晓勤 , 黄文彦 , 孙骅 , 吴元俊
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:观察姜黄素对体外培养的人肾小球系膜细胞(MC)增殖的影响.方法:采用不同浓度姜黄素处理体外培养的人肾小球系膜细胞,应用四甲基偶氮唑蓝(MTT)法测定系膜细胞活性(A值),并与地塞米松进行比较.结果:当姜黄素浓度≥6.25 μmol/L时,系膜细胞增殖明显受到抑制,且表现为浓度依赖性;当姜黄素浓度≥50 μmol/L时,姜黄素抑制系膜细胞增殖的作用明显强于地塞米松(P＜0.05).结论:姜黄素能抑制体外培养的人肾小球系膜细胞增殖,且作用强于地塞米松.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨转化生长因子(TGF)-βⅡ受体在SD大鼠胰腺发育后期的表达及意义.方法:使用RT-PCR方法分别检测交配后18.5天、新生及成年SD大鼠胰腺TGF-βⅡ受体mRNA表达;使用Western blot方法分别对上述不同时期SD大鼠胰腺TGF-βⅡ受体表达进行蛋白质水平的检测.结果:新生鼠胰腺TGF-βⅡ受体表达在核酸与蛋白质水平上均明显高于18.5天胎鼠与成年鼠胰腺,差异有显著性(P＜0.05).结论:TGF-βⅡ受体表达在新生鼠阶段有明显上调.
施韬 , 钱燕宁 , 王斌 , 马瑞 , 周建平 , 卞清明
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:观察氯化锂预处理对沙土鼠脑缺血-再灌注模型的神经元保护作用.方法:夹闭沙土鼠双侧颈总动脉5 min,制备前脑缺血性脑损伤模型.48只沙土鼠随机分为2组:实验组(A组,n=24)和对照组(B组,n=24),A组手术前连续5天给予氯化锂腹腔注射,B组以生理盐水代替氯化锂.每组又分为两个亚组:假手术组(Ash,Bsh,n均为12)和缺血组(Ais,Bis,n均为12),分别于术后3天(Ash3,Ais3,Bsh3,Bis3,n均为6)和7天(Ash7,Ais7,Bah7,Bis7,n均为6)断头取脑,制成石蜡切片,光镜下观察.结果:海马CA1区锥体细胞数目(每0.04mm2中含有的细胞数)分别为:Ais3组15.8±3.31、Ais7组13.54±5.22、Bis3组11.81±4.58、Bis7组1.48±1.55、Ash3组28.17±4.02、Ash7组28.11±4.14、Bsh3组29.64±5.99、Bsh7组30.21±2.95.Ais3组、Ais7组显著多于相应的Bis3组、Bis7组(P＜0.01),Ais3组、Bis3组显著多于相应的Ais7组、Bis7组(P＜0.01).结论:氯化锂预处理能明显减少沙土鼠脑缺血再灌注后海马CA1区延迟性神经元死亡.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨沙土鼠海马缺血耐受与CA1区蛋白激酶B(Akt)表达及锥体神经元凋亡之间的关系.方法:沙土鼠16只,随机分为A(2 min缺血组)、B(5 min缺血组)、C(2 min缺血+5 min缺血组)、D(假手术组)4组,每组4只.免疫组化(SP法)检测海马CA1区Akt表达,末端转移酶介导的缺口末端标记(TUNEL)法检测锥体细胞凋亡.结果:A、C两组表达的Akt阳性细胞数明显多于B组,D组无明显Akt表达;B组锥体细胞凋亡较A、C两组显著,D组未见明显凋亡细胞.结论:Akt可能通过抑制锥体细胞的凋亡参与介导沙土鼠海马缺血耐受.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:评价肺音图能否作为支气管激发试验的监测指标.方法:选取肺通气功能正常的缓解期支气管哮喘患者18例、健康对照者6例,进行组胺支气管激发试验;对支气管激发试验用药前、后肺音图参数即肺音强度(LSI)、最大峰值处的频率(PF)、功率谱曲线下面积划成4等份处的频率(Q25、Q50、Q75);进行自身对照研究,并比较支气管哮喘组与健康对照组激发前、后肺音图参数的变异率.结果:支气管哮喘组激发后PF、Q50(分别为186.4±120.5,296.5±210.5,P＜0.05和221.1±105.5,332.7±210.2,P＜0.02)均有显著性增高.健康对照组组胺激发前、后肺音图参数均无明显差异(P＜0.05).支气管哮喘组与健康对照组激发前、后肺音图参数的变异率△PF%、△Q50%在吸气相两组差异有显著性.结论:肺音图参数能灵敏地反映气道炎症所致的气道形态改变对呼吸音的产生与传导的影响,是敏感、有效的支气管激发试验监测指标.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:观察抗氧化剂亚硒酸钠(Se)对肿瘤坏死因子(TNF)-α诱导的大鼠血管平滑肌细胞(VSMC)细胞间黏附分子(ICAM)-1表达的影响,以探讨TNF-α介导单核细胞与VSMC黏附,致动脉粥样硬化及硒抗动脉粥样硬化的可能机制.方法:体外培养大鼠VSMC,分别加TNF-α(20 ng/ml)及TNF-α(20 ng/ml)+Se(0.5～2.0μmol/L),孵育24 h,采用细胞黏附实验、流式细胞术和RT-PCR测定ICAM-1蛋白及mRNA水平表达.结果:TNF-α可诱导VSMC ICAM-1表达增强;而硒可降低TNF-α诱导的ICAM-1 mRNA水平及蛋白表达,且呈浓度依赖性.结论:抗氧化剂硒可抑制TNF-α诱导的VSMC中ICAM-1的表达,减少单核细胞与VSMC黏附,这可能对延缓动脉粥样硬化的发生发展起一定作用.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:评价不同年龄健康人血压和动脉顺应性关系及其临床意义.方法:按美国明尼苏达大学方案将200名受试者分为4个年龄组:15～30岁;31～45岁;46～60岁和61～80岁.每组男女各25名,使用美国产HDI-2000动脉测试仪同步记录血压和动脉顺应性(大动脉弹性为C1,小动脉弹性为C2).结果:男性组:收缩压(SBP)和舒张压(DBP)均随年龄增加而升高,同时C1和C2顺应性下降,有统计学意义(P均＜0.01),但脉压差(PP)无统计学差异(P＞0.05);C1与SBP、DBP和PP呈显著负相关,其中与SBP相关性最好(r=-0.640,P＜0.01);C2与SBP和DBP呈显著负相关,其中与DBP相关性最好(r=-0.544,P＜0.01),与PP无相关.女性组:SBP、DBP和PP随年龄增加而升高,C1和C2顺应性下降,有统计学差异(P均＜0.01).C1和C2均与SBP、DBP和PP呈显著负相关,其中与SBP相关性最好(r=-0.660,P＜0.01;r=-0.704,P＜0.01).结论:男性和女性随着年龄的增加,大、小动脉顺应性均下降.SBP是影响其大、小动脉顺应性的最主要因素.
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:研究二亚乙基三胺(DETA)迟发预处理反应(DPR)对低温保存的心脏移植供体的保护作用并探讨其可能机制.方法:32只青紫兰兔随机分成2组.一组作为移植受体组(n=16);另一组作为移植供体组,随机分成A、B 2个亚组(n=8),A组为预处理组,B组为对照组.A、B两组获取心脏后低温保存供体4 h,再移植到受体组建立心脏腹腔异位移植模型.检测受体组移植后心功能的恢复情况;心肌活性氧(ROS)、丙二醛(MDA)含量、Na+-K+-ATP酶活性;冠状动脉回血中的肌酸激酶(CK)、乳酸脱氨酶(LDH)、乳酸(LD)含量.结果:和B组相比DETA预处理组(A组)的供体有较好的心功能恢复,较少的CK、LDH的漏出,较高的Na+-K+-ATP酶活性,较少ROS、MDA、LD的生成.结论:DETA诱导的DPR,在心脏的获取-低温保存-移植后再灌注过程中,对心肌细胞有显著的保护作用.
贾瑞鹏 , 马庆铮 , 刘军 , 程继义 , 许纯孝 , 王万忠 , 杜德利 , 姜玉菲
2003, 23(3). DOI: 10.7655 CSTR:
Abstract:目的:探讨肾癌组织内微血管密度(IMD)、DNA含量的临床意义.方法:采用免疫组织化学(LSAB法)方法及图像分析技术对76例肾癌患者手术切除的标本进行IMD和DNA含量测定,并分析其与预后间的关系.结果:不同肾癌细胞类型、病理分级之间IMD值比较差异无显著性(P＞0.05);随临床分期的升高,IMD增高(P＜0.05),转移组IMD值明显高于非转移组(P＜0.01);随访5年内死亡者IMD、DNA含量均高于存活5年以上者(P＜0.05).Spearman相关分析研究表明:IMD与DNA蛋白含量具正相关性.结论:IMD值的变化与肾癌恶性程度相关,较高的IMD和DNA含量常提示患者预后较差.
楚瑞琦 , 曹峻岭 , 张军芳 , 谢龙 , 岳燕 , 胡小平 , 李思远 , 谭升顺
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To investigate the possible effect of nivalenol on metabolism of the cultured chondrocytes and the protection of selenium. Methods:The quantitative analyses of metabolism in single-layer cultured chondrocytes were performed by biochemical means and the impairment of DNA was observed by both of the single cell microgel electrophoresis assay and the agarose gel electrophoresis assay. Results: In the media containing different concentrations of nivalenol (0. 000 5-0. 020 0 mg/L), the amounts of DNA and proteoglycan in matrix of the chondrocytes were decreased. The synthesis of protein was reduced and the impairment of DNA deteriorated with the increase of the concentrations of nivalenol in the given dose. When selenium was added into the media, the impairment by nivalenol was decreased. In the media containing different concentrations of nivalenol, however, the lipid peroxidation of the chondrocytes was not affected by nivalenol, yet the amount of lipid peroxides significantly declined. Conclusion: Nivalenol may evidently cause impairment of the chondrocytes when its concentrations are in the present experimental range. Selenium can protect cultured chondrocytes, but cannot prevent their DNA from being impaired.
程虹 , 丁新生 , 王琨 , 张雪玲 , 王颖 , 姚娟 , 邓晓萱
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective:To evaluate the diagnostic potential of cerebrospinal fluid ( CSF) levels of β-amyloid protein (Aβ) as biochemical marker for senile dementia in clinical practice. Methods: Sensitive enzyme-linked immunosorbent assay ( ELISA) was performed in our laboratory to detect the CSF levels of Aβ1-40, Aβ1-42 in 54 patients with Alzheimer's disease (AD), and 30 normal controls (NC). Results: The cut off value of Aβ ratio and Aβ1-42 concentration in NC group provided 54. 51％, 90. 00％ sensitivity and 81. 25％, 84. 38％ specificity respectively in diagnosis of AD. Conclusion: AD group bad a significant decreased level of Aβ1-42 and an increased level of Aβ ratio, compared with NC group.
许迪 , 孔祥清 , 杨荣 , 盛燕辉 , 曹克将 , 陆凤翔
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To evaluate of the role of transesophageal echocardiography (TEE)in percutaneous closure of atrial septal defects (ASD) with the Amplatzer septal occluder. Methods:Sixty-two patients (10 to 55 years of age) were selected for percutaneous closure of ASD by transesophageal echocardiography, which was also used to monitor the procedure, to select the appropriate sizeof the Amplatzer device, to verify its position, and to access the immediate results of the procedure.During the follow-up, transthoracic echocardiography ( TTE) or TEE was used to evaluate the presence and magnitude of residual shunt (RS), device position, and right cardiac chamber diameters. Results: The mean ASD diameter by TTE ( [ 19.1 ± 5.8] mm ) was significantly smaller ( P ＜0.001 ) than the stretched diameter of the ASD (25.1±6.4) mm. There are no significant differences between the TEE-measured value (23.5±6.2) mm and the stretched diameter of the ASD ( P＞ 0.05). Due to proper patient selection all procedures were successful. There was immediate and complete closure in 61/62 patients, only one patients had trivial residual shunt. Follow-up was performed using TTE or TEE right after operation, 1 d, 1 month, 3 months, 6 months and yearly thereafter. All patients remain asymptomatic without any clinical or technical problems. Conclusion: With the aid of TEE, percutaneous closure of ASD can be performed successfully, safely, and effectively.
Yakubu M Bello , Odama LE , Nandita B De
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective : To investigate the scientific bases for the traditional use of Stachytarpheta angustifolia. Methods: In vitro antibacterial activity of the aqueous and ethanol extract of the plant was investigated using the agar cup plate diffusion method. Results: The ethanol extract of the plant shoued antibacterial activity against Escherichia coli , Streptococcus faecalis , Shigella dysenteriae ,Staphylococcus aureus ( S. Aureus ), Salmonella sp. , Pseudomonas aeruginosa and Neisseria gonorrhoeae, while the water extract was active against Escherichia coli, Streptococcus faecalis, Shigella dysenteriae, Staphylococcus aureus and. Pseudomonas aeruginosa. The ethanol extract exhibited higher antibacterial activity than the water extract. The minimum inhibitory concentration (MIC) and minimum bacterwidal concentration ( M BC ) of the ethanol extract were 0.65 mg / ml and 0.85 mg / ml , respectively, against S. Aureus. Treatment of the extract at higher temperature, 60 ℃ increased the sensitivity of the test organisms to the plant extract. Phytochemical analysis indicated that the plant possesses tannins, saponins as well as phenols. Conclusion: A scientific basis exists that the plant possesses antibacterial activity and it could be a probable source of therapeutic agent.
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To investigate the expression and clinical significance of Fas and FasL in lung cancer. Methods: SP immunohistochemical technique was used to detect the expression of Fas and FasL in 46 cases of lung cancer and 30 cases of adjacent non-neoplastic tissue. Results: Down-regulation of Fas and up-regulation of FasL were found in lung carcinoma. The levels of Fas expression in squamous cell carcinoma, adenocarcinoma and SCLC were significantly lower than that of adjacent normal tissues ( P＜ 0. 01), while the expression levels of FasL were the opposite (P＜0. 05). Fas expression was associated with high histological grade and no metastasis (P＜ 0.05).FasL expression was related to histological grade, late clinical stage and metastasis ( P＜ 0.05). Both Fas and FasL expression was not related to the histological type of lung cancer ( P＞ 0.05). The level of Fas expression was negatively related to that of FasL ( P ＜ 0.05). Conclusion :Down-regulation of Fas and up-regulation of FasL may work in coordination with the occurrence, development and metastasis of lung cancer. Fas or FasL can be used as one of markers in early diagnosis of lung cancer.Therefore, the combined assay may be helpful in predicting the grade of malignancy and the prognosis of patients with lung cancer.
王道荣 , 陈国玉 , 刘训良 , 苗毅 , 夏建国 , 武正炎
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To study the expression of CD44 correlation with the ability of metastasis of tumor cells in gastric carcinoma, to find the correlation of the concentration of soluble CD44 Variant 6 (sCD44v6) and the histologic expression of CD44 Variant 6 ( CD44v6 ) in tumors with the clinico-pathologic features, and to make serum concentration of the CD44v6 and the expression of CD 4 4v6 nay be useful an indicator as an early diagnosis, invasion, metastasis, and prognosis. Methods: Serum samples were obtained from 70 patients with primary gastric carcinoma before surgery and 20 patients after surgery. Serum levels of CD44v6 were determined with a quantitative-enzyme-linkimmuno-adsorbent assay. The expression of CD44v6 in tumors was examined by immuno-histo-chemical staining. Results: Both the serum concentration of CD44v6 and its expression in tumors were positively related to the depth of invasion of the tumor, lymph node metastasis, clinical stage, and diffuse type gastric carcinoma, but not to the tumor size. The serum level of CD44v6 in patients with gastric cancer was significantly higher than that in control. The serum concentration of CD44v6 was markedly lowered after curative surgery ( P＜ 0. 001), and the serum level of CD44v6 was higher in patients with gastric cancer with CD44v6 positive tumor cells than in those with CD44v6 negative tumor cells. The serum level of CD44v6 was a prognostic indicator in patients with diffuse type gastric carcinoma, as was the histological expression of CD44v6. Conclusion: CD44v6 of gastric cancer and serum concentration of CD44v6 seems to be correlated to the progression of diffuse type gastric carcinoma metastasis and clinical stage. An elevated level of sCD44v6 may serve as an indicator of lymph node metastasis(especially early metastasis) and bad prognosis in patients with gastric carcinoma.
钱立新 , 程双管 , 眭元庚 , 何戎华 , 吴宏飞 , 张炜 , 李强
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To inquire into diagnosis, and treatment of virilizing and feminizing adrenal syndrome, differential diagnosis between benign and malignant sex hormone producing adrenal neoplasma and, treatment principles of congenital adrenal hyperplasia ( CAH ) . Methods: Eight cases of CAH and 5 cases of sex hormone producing adrenal neoplasma were admitted to hospital during 1986-1996. The former included 3 rare cases of 17 a hydroxylase deficiency. The latter included 3 cases of feminizing adrenal tumor and 2 cases of virilizing adrenal tumor. Results: Weight, size and CT of the tumor, DHEA, 17-ks, sex hormone levels, infiltration, and metastasis were closely related to the degree of differentiation of the tumors. Conclusion: Virilizing and feminizing adrenal neoplasm were removed surgically by different incisions. Modified subcostal incision was recommended as the best choice for huge adrenal mass. Corticoadrenal hormone treatment fo CAH should be individualized according to the different types of the disease. Sex hormones were not suitable for children suffering from 17 hydroxylase deficiency before puberty.
2003, 17(3). DOI: 10.7655 CSTR:
Abstract:Objective: To investigate the electromagnetic field and specific absorption rate(SAR) distribution of different structure applicators with different depths for treating bone tumors using microwave hyperthermia. Methods: The finite element method ( FEM ) was used to calculate dectromagnetic field and SAR distribution. Two different structure applicators were simulated. The one is simple coaxial antenna, which has been successfully used in clinic treating bone tumors in Tangda hospital of the Forth Military Medical University several years. It was formed by a coaxial cable peeled off the out copper at end. The other applicator was coaxial-slot antenna, which was widely used in microwave hyperthermia. The applicator inserted into the cylindrical bone with different depths, and worked at the frequency of 2 450 MHz. Results: The electric field and SAR generated by the simple coaxial applicator were mainly concentrated out the tissues, and were not uniform in the tissues, while the coaxial-slot applicator well transmits the electric field and SAR into the tissues, and can easily treat different position by adjusting the slot position. Conclusion:The results calculated by EFM, were well accordant with the experimental and clinical results, and will be important for improving the clinical effects of microwave hyperthermia.