Abstract:Objective： To obtain the completely humanized Fab antibody against idiotypic antibody from a phage-display library of schistosoma japonicum. Methods： A schistosoma japonicum library of phage-display human Fab library was constructed using genes from spleens of 3 patients with schistosoma japonicum infection. Soluble egg antigen（SEA） and soluble worm antigen preparations （SWAP） were used to screen schistosoma japonicum immune mouse serum IgG. The positive recombinant phages identified by ELISA were used to infect E.coli TOP10 for the production of soluble Fab antibodies. Results： A schistosoma japonicum human Fab phage-display library consisting of 4.3 × 106 clones was successfully constructed. The sequencing results demonstrated that it was a human Fab gene library. After five rounds of panning， sixty randomly selected clones from the enriched phage library were tested with ELISA， and four clones（A3，B6，C4，C17） were found to bind to IMS （Ab1） but not to SEA and SWAP. The clone B6 was expressed and identified by SDS-PAGE. Sequencing was also carried out. Conclusion：A schistosoma japonicum human Fab phage-display library could be successfully constructed， from which anti-idiotypic antibody （B6） of schistosoma japonicum is obtained. And it is useful for further study on anti-idiotypic antibody vaccine development of schistosoma japonicum.

Abstract:Objective： To study the expression and function of 4-1BBL in the cell lines of human hematological malignancies. Methods： The expression of 4-1BBL was detected by FACS analysis and the effects of 4-1BBL molecule in the cell lines of human hematological malignancies were studied by anti-4-1BBL mAb 1F1. Cell proliferation was determined by cell counts manually and 3H-TdR incorporation. Results： FACS analysis showed that 4-1BBL expressed highly in myeloid leukemia cell line U937， HL60 and B Lymphoma cell lines Raji， Daudi. Cell counts and 3H-TdR incorporation assay revealed that mAb 1F1 could induce proliferation of myeloid leukemia cell line U937 and HL60. Conclusion： 4-1BBL molecule is expressed in the myeloid leukemia highly as well as B lymphoma cell lines. 4-1BBL molecule could mediate reverse signal to promote the proliferation of myelocytic leukemia cell line U937 and HL60.

Abstract:Objective： To investigate the expression of CD2-associated protein（CD2AP） and α-actinin-4 in rats with adriamycin nephropathy， and to explore their possible effects on the development of proteinuria. Methods： Thirty SD rats， weighing from 140 to 160 g， were randomly divided into nephropathy model group and normal control group. The nephropathy model was established by a single intravenous injection of adriamycin（6 mg/kg）. Normal control group received the same volume of 0.9% sodium chloride solution. At the end of the 2nd， 4th， 6th and 8th week after injection， the rats were sacrificed and their kidneys were acquired. The renal histology was observed by light microscope and electronic microscope. The semiquantitative western blot was used to detect the levels of CD2AP and α-actinin-4. Results： The protein levels of CD2AP in nephropathy model groups at the end of 2nd， 4th and 6th week were 3.0， 1.8 and 1.2 times of those in normal control group respectively. However， no appreciable alterations were observed in the expression level of α-actinin-4 at any time point. Conclusion： The expression of CD2AP is associated with the development of proteinuria in adriamycin nephropathy rats. But there is no relationship between the expression of α-actinin-4 and the increase of proteinuria.

Abstract:Objective： To investigate the role of Notch1／Jagged1 activation pathway in the development of renal tubulointerstitial fibrosis in vivo. Methods： Rats were sacrificed after1 day， 3 days， 7 days and 14 days of ureteral obstruction（UUO）， and sham-operated rats were sacrificed as the control. Notch1／Jagged1 mRNA and protein and Fibronectin （FN） were analyzed with semi-quantitative RT-PCR and western blot. Results： Notch1／Jagged1， FN mRNA and protein levels of the UUO group at the 1st， 3rd， 7th， 14th days in the kidneys of rats with obstructed kidneys， were significantly increased （P < 0.05 or P < 0.01）， compared to sham-operated rats. Notch1／Jagged1 mRNA expression were peaked 7 days after UUO （P < 0.05）. Jagged1 protein expression was peaked 1 day after UUO. The levels of Notch1 and FN protein expression were peaked 14 days after UUO（P < 0.05）. Conclusion： Expressions of Notch1／Jagged1 are increased in renal tissue after UUO. Notch1／Jagged1 signaling pathway may be involved in UUO rats and plays a concordant role in the pathogenesis of renal interstitial fibrosis.

Abstract:Objective： To study NF-κB activation during the early phase of T cell reaction due to CD3／CD28 co-stimulation and the effect of arsenic trioxide on NF-κB. Methods： Hut-78 cells were stimulated with CD3／CD28 monoclonal antibodies（10 μg／ml and 5 μg／ml， respectively） in 30 min. TransAM was employed to investigate p65 expression in nuclear extracts and the effect of arsenic troxide. Results： The level of p65 in nuclear extracts of Hut-78 cells was much lower during the CD3／CD28 co-stimulation in 30 min， however arsenic trioxide seemed to inhibit the effect of CD3／CD28 co-stimulation. And the trend was nearly the same in whole cell lysates. Conclusion： NF-κB is suppressed during the early phase of CD3／CD28 co-stimulation in human T cells.

Abstract:Objective： To establish the bronchial asthma model in sprague dawley（SD） rat. Methods： Twenty-four SD rats（SPF）were randomly divided into normal control group and asthmatic model group，12 rats per group. The asthmatic model of SD rats were established by immunization with intraperitoneally injected and inhaled ovalbumin（OVA）. The bronchial provocation test by acetylcholine was used in each group. Expiratory airway resistance（Re）was measured by using animal respirator. The thicknesses of WA／Pi，ASM／Pi，and the numbers of eosinophils and lymphocytes were measured. Results： Through the comparison of Re，the thicknesses of WA／Pi and ASM/Pi， and the numbers of eosinophils of each group， the modeling method was confirmed to be successful. The analysis of correlation indicated that Re of asthmatic model group was positively correlated with the thicknesses of WA／Pi and ASM／Pi and the numbers of eosinophils and lymphocytes while challenged by acetylcholine（80 μg／kg and 160 μg／kg）. Conclusion： The bronchial asthma model in SD Rat might be established successfully. The success of the modeling method might be evaluated directly by the concentration doubling of Re， while challenged by acetylcholine of 80 μg／kg and 160 μg／kg.

Abstract:Objective：To investigate the effects of AngII antagonist valsartan on airway inflammation and airway hyperresponsiveness（AHR） in asthmatic rats. Methods: Fifty Wistar rats were divided into 5 groups, group A（normal control group）, group B（asthma group）, group C ［treated with valsartan, 15mg／（kg·d）］, group D［treated with valsartan, 30 mg／（kg·d）］，and group E ［treated with valsartan, 50 mg／（kg·d）］, with 10 rats in each group. Pulmonary function tests were performed to evaluate the airway responsiveness, and bronchial alveolar lavage fluid（BALF） was used to analyze the total cell and eosinophil（EOS） counts. Results: Compared with group A , group B had an increased count of total cell and EOS in BALF（P ＜ 0.05，P ＜ 0.01) . Compared with group B, group C, D, E showed a decreased count of total cell and EOS in BALF（P ＜ 0.05-0.01). Compared with group C, group D had a significantly decreased count of total cell and EOS（P ＜ 0.01) in BALF. After stimulation of methicholine with accelerated concentrations（40，80，160 μg／ml）, the mean expiratory resistance（Re） of group B was higher than that of group A, C, D, E（P < 0.05). When the concentration of methicholine was 320 μg／ml, the asthmatic group showed a significant difference. Conclusion: Valsartan may inhibit airway inflammation and reduce airway responsiveness by suppressing AT1 receptors.

Abstract:Objective： To evaluate the influence of local delivery of tissue factor pathway inhibitor gene on intimal hyperplasia after balloon injury. Methods： Thirty-six male New Zealand white rabbits were divided into three groups， saline， adenovirus-defect and TFPI gene treated group. Balloon catheter was used to induce injury to the carotid artery of rabbits and saline， adenovirus-defect and TFPI gene were locally delivered on injury site. Fourteen days after operation， the local injury vessel was removed and harvested for pathological analysis and immunohistochemical staining. Automatic image analysis system was used to detect the changes of intimal, medium, luminal area， and the proliferation index of proliferating cell nuclear antigen（PCNA）. Results： Morphological analysis showed intimal hyperplasia remarkably decrease， along with the proliferation index of PCNA significantly reduction and luminal area augment significantly in TFPI gene treated group compared with saline and adenovirus-defect group. Conclusion： Local TFPI gene transferring may reduce the expression of PCNA，and decrease the intimal hyperplasia and enlarge the luminal area in carotid artery of rabbits.

Abstract:Objective： To investigate the coronary sinus（CS） electrogram and the relationship of CS electrogram with local electrical activation of the coronary sinus muscle sleeve（CSMS）， and to demonstrate that A wave of CS electrogram was not only an atrial activating wave， but a complex wave of CSMS potential and left atrial（LA） potential. Methods：Forty-two patients with paroxysmal supraventricular tachycardia（PSVT） were involved. Under sinus rhythm， RS2 stimuli were issued at the proximal CS electrodes（CS9，10）， and then decreased by 5 ms steps. A wave morphology， changing of its order and pattern， double potential and activating time of CS were observed. Results：During sinus rhythm， A wave of distal CS（CS1， 2） in 67% patients showed firstly down and nextly up direction， with broad pattern and multiple compositions. RS2 firstly captured A wave in proximal CS， made it advance in time. Gradually with the decrease of RS2， A wave of CS in the middle segment also advanced， and its pattern changed into firstly down then up direction. When RS2 was early enough， A wave of distal CS（CS1，2） changed into first up then down direction. Conclusion： A wave of CS is not a pure atrial wave， but a potential fusion of CSMS and LA. During sinus rhythm， proximal and middle CS is activated mainly by the pulse through Bachmann bundle and CS， LA potential fuses with CSMS potential. The distal CS is activated by the pulse through Bachmann bundle. Under some conditions， two electric potentials could separate.

Abstract:Objective： To explore the pathophysiological changes of chronic heart failure resulting from pressure overload in juvenile and the effects of carvedilol. Methods： The animal model of CHF was established by constriction of abdominal aorta. Four weeks after operation， the rats were randomly divided into 3 groups： sham-operated group， CHF group， carvedilol group. Carvedilol was administered by direct gastric gavage. Eight weeks after operation， the hemodynamic analysis， the pathologic analysis of hearts and the cardiac myocyte apoptosis analysis were performed. The serum contents of lipid pereoxidation（LPO） and superoxide dismutase（SOD） were detected. Results： Compared with the sham-operated group， SBP， DBP， LVSP， LVEDP， LVRW， RVRW， LPO， AI were all significantly increased（P < 0.01） in CHF group. A significant decrease could be seen in +dp／dtmax， -dp／dtmax， SOD in CHF group. A significant decrease could be seen in SBP， DBP， LVSP， LVEDP， LVRW， RVRW， LPO， AI in carvedilol group compared with CHF group. While a significant increase could be seen in +dp／dtmax， -dp／dtmax and SOD（P < 0.01）. Conclusion：The ventricular remodeling， the myocardial apoptosis and oxidative stress may take part in the pathogenesis and progression of CHF， which might be inhibited by carvedilol.

Abstract:Objective： To evaluate the effects of cold preservation on hepatocyte proliferation in a novel model of partial liver transplantation in rats. Methods： Donor livers were preserved at 0-4℃ for 30 minutes， 4 hours and 10 hours in University of Wisconsin solution. The expression of the proliferative cell nuclear antigen（PCNA）， necrosis factor alpha（TNF-alpha） and interleukin 6（IL-6） of liver in rats were evaluated by immune histochemical method 1，2，4 d after operation， respectively. Results：30-minute， 4-hour and 10-hour preservation before 50% partial transplantation resulted in 79%， 71% and 29% animal survival， respectively. Prolonged time of cold preservation（10 h） was associated with a dramatic decrease of the marker of regeneration（P < 0.05）. TNF-alpha and IL-6 was significantly decreased in recipient rats compared with the other groups. Conclusion：Periods of cold preservation are significantly associated with the regenerative ability of the liver and animal survival. Cold preservation decreases the expression of TNF-alpha and IL-6 production in graft. They play a primary role in modulating hepatocyte proliferation in the cold ischemic liver.

Abstract:Objective： To explore the relationship between microsatellite DNA polymorphism of human 11beta-hydroxysteroid dehydrogenases type 1 and type 2 diabetes within a Chinese population. Methods：150 patients with type 2 diabetes and 121 nondiabetic subjects were collected randomly among Han Chinese in Nanjing. Polymorphism of microsatellite marker within intron 4 of 11β-HSD1 was detected by polymerase chain reaction， polyacrylamide gel electrophoresis and silver staining to investigate the frequency distributions of 11beta-HSD1 microsatellite（CA）n in two groups. Results： In two groups， CA-repeat numbers in eight types of alleles were 13，14，15，16，17，18，19 and 20， respectively. The prevalence of 11beta-HSD gene allele frequencies in patients with type 2 diabetes was not significantly different from that in control subjects（χ2 = 8.9944， P = 0.253）. However， the prevalence of （CA）15 allele gene frequencies in patients with type 2 diabetes was significantly different from that in control subjects（χ2 = 4.990，P = 0.025）. Conclusion： Polymorphism of microsatellite markers in 11beta-HSD1 may be associated with type 2 diabetes in Han Chinese.

Abstract:Objective： To explore the relationship between microsatellite DNA polymorphism of human PON1 gene and type 2 diabetes mellitus in Chinese population of Nanjing. Methods： Among Chinese people of Han nationality in Nanjing，119 patients with type 2 diabetes mellitus and 116 nondiabetic subjects were collected randomly. Polymorphism of microsatellite marker was detected by polymerase chain reaction，polyacrylaimde gel electrophoresis and silver staining to investigate the frequency distribution of PON1 gene microsatellite（GT） in patients with type 2 diabetes mellitus or nondiabetic subjects. Results：The GT sequences in four types of alleles were repeated for 12，15，17，20 respectively. The PON1 gene alleles frequencies in patients with type 2 diabetes mellitus were significantly different from that in control subject（χ2 = 14.10，P < 0.01）. Conclusion：polymorphism of microsatellite markers in PON1 gene may be associated with type 2 diabetes in Han people.

Abstract:Objective： To explore the relationship between the parameter of P1 wave elicited by pattern stimuli， spatial frequency and visual acuity in traumatic optic neuropathy. Methods：Forty-two cases of traumatic optic neuropathy were enrolled from West China Hospital， Sichuan University. The reversal pattern grating of five check size（117， 58， 29， 14， 7 min of arc） were used as stimuli. The reversal rate of the stimuli was 2.9 Hz， and the contrast level was 100%. The latency and amplitude of P1 wave were recorded while the threshold stimulus was given. SPSS statistical software was used to describe the datum and analyze the relationship between the spatial frequency and visual acuity. Results： There were high correlation between spatial frequency of stimulate threshold and visual acuity， and between the latency of P1 wave and the visual acuity in all the checked eyes. Conclusion： PRVEPs can be used to evaluate the visual acuity.

Abstract:Objective： To explore the effects of dipeptide-supplemented total prenteral nutrition（TPN）， traditional TPN on the function change of liver graft after orthotopic liver transplantation. Methods：Thirty-five patients were randomly divided into three groups： diet group（receiving common diet）， traditional group（receiving TPN without Ala-Gln）， dipeptide group（receiving TPN and Ala-Gln）. Patients in traditional group and dipeptide group received isocaloric［104.6 kJ／（kg·d）］ and isonitrogenous［0.16 g／（kg·d）］ on the second day to the eighth day after transplantation. Liver function-related index was measured on the second day and the ninth day after liver transplantation respectively. Results： Aspartate aminotransferase（AST） of dipeptide group decreased more significantly compared with diet and traditional group（P < 0.01，P < 0.05）；Apolipoprotein B（APOB） of traditional group and dipeptide group increased more significantly compared with diet group（P < 0.01）；Cholinesterase（CHE） of dipeptide group increased more significantly compared with diet and traditional group（P < 0.01）on the ninth day after OLT. Conclusion：TPN improves the function and recovery of ischemia reperfusion injury of graft. Ala-Gln increases the effects of TPN.

Abstract:Objective: To evaluate the therapeutic effects of high dose pulse oral calcitro on moderate and severe secondary hyperparathyroidism patients. Methods: Two micrograms calcitrol twice a week were administered to 93 patients with secondary hyperparathyroidism whose intact PTH was more than 500 pg／ml, refractory to conventional calcitrol therapy（oral 0.25 micrograms calcitrol in each day for 8 weeks）. The clinical feature of observed patients, including sex, age, dialysis duration, serum calcium, phosphorus, iPTH and AKP level were analyzed. Results: Serum iPTH level decreased obviously in 74 patients in 8 weeks after high dose pulse treatment（P < 0.01）. Elevated calcium × phosphorus product occurred in 19 patients and the pulse treatment was stopped. Seventeen patients accepted total parathyroidectomy with forearm transplantation for hyperplastic parathyroids detected by ultrasound and ECT. Conclusion: High dose pulse oral calcitrol was effective on moderate and severe secondary hyperparathyroidism. The patients with obviously hyperplastic glands need the parathyroid operation.

Abstract:Objective： To explore the indication and experiences of Pemberton’s pericapsular osteotomy of the ilium in the treatment of developmental dislocation of the hip in children. Methods： From June 1999， 192 hips in 165 patients（31 male， 134 female） with developmental dislocation of the hip underwent Pemberton’s pericapsular osteotomy of the ilium， and 79 cases underwent the operation on left hip， 67 cases on right hip， 23 cases on both hips. According to T-觟nnis classification， 12 hips were graded as Ⅰ°， 46 hips as Ⅱ°， 95 hips as Ⅲ°， 39 hips as Ⅳ°. Results：All patients were followed up for a period from 4 months to 7 years （mean， 3 years and 2 months）， and were assessed clinically and radiologically. Clinical assessment was performed according to Mckay’s classification. The results showed that the overall excellent or good rate was 92.71%. Radiological assessment was performed according to Severin’s classification， and the results showed that the overall excellent or good rate was 91.66%. Conclusion： Pemberton’s pericapsular osteotomy of the ilium is an effective method to treat developmental dislocation of the hip in children.

Abstract:Objective： To explore the clinical characteristics of leiomyosarcoma（LMS） and the factors influencing prognosis. Methods： The clinical data of 27 patients with LMS were retrospectively analyzed and summarized， including clinical features， pathological type， curative effect and prognosis. Results： The patients with LMS were most aged between 40 to 60 years old. The main symptom of LMS was irregular menses（29.6％）， followed by climacteric irregular colporrhagia（18.5％）， celialgia（18.5％） and bythus lump（11.1％）. The diagnosis rate before operation and during operation was 25.9%; 17 cases were misdiagnosed as hysteromyoma or uterus adenomyosis， 3 cases were misdiagnosised as tumor of the ovary， and the misdiagnosis rate was 74.1%. Eight cases at stageⅠwere treated by surgery simply, and 19 cases at stageⅡ，Ⅲ and Ⅳ were treated by surgery combined with chemotherapy. The 5-year survival rate of the patients at stageⅠwas 87.5%. The average 5-year survival rate of patients behind stageⅡwas 42.1％. The 5-year survival rate of patients at stageⅡ， Ⅲand Ⅳ was 70.0％， 20.0%， 0%，respectively. Conclusion： LMS could be easily misdiagnosed. It was fairly difficult to make an early diagnosis. The main treatment method of LMS was surgery. The prognosis of LMS is obviously related with age and clinical staging. To reduce recurrent and to increase survival rate， it is important to confirm the diagnosis before or during operation and combine with adjunctive therapy after surgery.

Abstract:Objective： To explore the effects of H. pylori infection on gastric epithelial cell kinetics and the expression of apoptosis related genes. Methods：Ｏne hundred and thirty-seven persons were enrolled and biopsy／operation specimens were taken for H. pylori detection by culture and histological assessments. Apoptotic cells in the specimens were counted in situ by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL） method. Epithelial cell proliferation was detected by immunohistochemical analysis of the proliferating cell nuclear antigen（PCNA）. The expressions of apoptosis-related gene Bax， bcl-2， and c-myc were examined by immunohistochemistry in biopsy specimens of 30 patients with H. pylori-infected chronic superficial gastritis and 10 uninfected patients with chronic superficial gastritis. Results： Of 137 persons，104 showed positive H. pylori. H. pylori infected patients with GU， DU had significantly higher apoptosis／proliferation ratio than persons with normal mucosa（P ＜ 0.01）， and H.pylori（+） patients with CAG， gastric cancer had significantly lower apoptosis／proliferation ratio than persons with normal mucosa（P ＜ 0.01）. But there was no difference between H.pylori（+） patients with CSG and persons with normal mucosa（P ＞ 0.05）. Bax and c-Myc in H.pylori（+） chronic superficial gastritis were significantly higher expressed than that in H.pylori（-） chronic superficial gastritis（P ＜ 0.01）. The positive staining of Bcl-2 was not found both in H.pylori（+） group and H.pylori（-） group. Conclution： H.pylori may affect apoptosis through altering the expression of apoptosis-related gene Bax and c-myc with the result of imbalance between gastric epithelial apoptosis and proliferation， which might be one part of the pathogenetic mechanisms of H.pylori infection.

Abstract:Objective： To investigate the relationship between reflux of acid／bilirubin and reflux， dyspepsia and symptoms from non-gastrointestinal tract， and to study the role of 24 h esophageal pH and bilitec monitoring in evaluating the new method of esophageal operation. Methods: Using the method of 24 h esophageal pH and bilitec monitoring， the acid and bilirubin in esophagus from 68 patients with typical reflux， 50 patients with dyspepsia； 20 patients with symptoms from non-gastrointestinal tract， and 32 patients undergoing esophageal operation were measured. Results：Of 68 patients with typical reflux， 65 presented with reflux episodes， with an incidence of 95.6%. Of 50 patients with dyspepsia symptoms，the incidence of reflux episodes was 72%. In patients with typical reflux， 51.4% presented with mixture reflux of acid and duodenal liquid. The incidence of reflux incidence in patients with new method of esophageal operation（preserving cardia and utilizing colon instead of esophagus） was lower， compared with the routine method（29.4% vs 66.7%，P < 0.05）. Conclusion： 24 h esophageal pH and bilitec monitoring provide an objective and effective method not only to diagnosis gastroesophageal reflux and duodenal-gastroesophageal reflux but also to evaluate the curative effect of the new operation method.

Abstract:Objective： To evaluate the clinical significance of serum sICAM-1 and bFGF change in digestive system cancers before and after intra-arterial chemotherapy. Methods：The levels of sICAM-1and bFGF of 35 patients with digestive system cancers before and after intra-arterial chemotherapy were measured by enzyme linked immunosorbent assay（ELISA） and compared with those of healthy controls. Results：The levels of sICAM-1 and bFGF of patients with digestive system cancers before intra-arterial chemotherapy were higher than those of healthy controls（P < 0.01）， and were correlated to metastasis significantly（P < 0.05）. After intra-arterial chemotherapy， the levels of sICAM-1 and bFGF were decreased（P < 0.01）. All of the patients with high level of serum sICAM-1 and bFGF before intra-arterial chemotherapy had presented with new metastasis six months later. Conclusion：Intra-arterial chemotherapy can reduce the levels of sICAM-1 and bFGF in patients with digestive system cancers. Codetermination of sICAM-1 and bFGF may be helpful to judge prognosis.

Abstract:Objective： To investigate the change of TDI parameters after right ventricular pacing， and to reveal the effects of VVI and DDD on myocardial contractile function. Methods：The study included 3 groups， VVI group（30 patients receiving single-chamber pacing）， DDD group（32 patients receiving dual-chamber pacing），control group（32 healthy human）. TDI parameters of regional myocardium were observed. Results：PSV， PSS and PSSR were lowest in VVI group， followed by DDD group and control group. The number of the segments with significantly decreased PSSR was less than that of the segments with decreased PSV and PSR. In the two pacing groups， PSVT， PSST and PSSRT of left ventricle prolonged significantly， but those of right ventricle were shortened. Conclusion：TDI is a noninvasive method to evaluate the regional myocardium contraction after recent pacing. After pacing， there is no significant change in strain rate， and the decrease of velocity and strain are most in left ventricle. It is suggested that there is no significant change of the left ventricular contraction after pacing， but the contraction of the right ventricle in VVI mode is decreased compared with DDD mode.

Abstract:Objective: To evaluate the early and med-term outcomes of transplantation of autologous bone marrow mesenchymal stem cells（BMSCs） and mononucleus cells（BMMNCs） to treat coronary heart disease. Methods: Fourteen patients with coronary heart disease complicated with myocardial infarction were enrolled in this prospective, nonrandomized, control study（8 patients in cell transplantation group, 6 in control group）. BMSCs and BMMNCs were isolated and cultured for 2-3 weeks, and （0.9-3.5） × 106 BMSCs plus（1.6-6.1） × 106 BMMNCs were transplanted into the myocardial infracted area in cell transplantation group through intracoronary way. All patients underwent 3-month and 8-month follow-up for 6 minutes test, 2D Doppler echocardiogram and SPECT. Results: After 3 months and 8 months follow-up, cardiac function was improved. Six minutes distance was prolonged from 320.72 ± 60.47 to 406.88 ± 68.31 and 484.79 ± 81.00. Left ventricular ejection fraction（LVEF） was increased from 42.5% to 49.5% and 54%. The infarct defected regions was reduced from 45.5% to 39% and 25% in 6 patients with anterior myocardial infarction. In control group, 2D Doppler echocardiogram and SPECT were improved in 3 months but not improved 8 months after PCI. In cell transplantation group, 2 patients with inferior myocardial infarction had no significant efficacy. Conclusion: The preliminary study showed that in the patients with coronary heart disease complicated with anterior myocardial infarction, transplantation of BMSCs and BMMNCs could improve cardiac function and cardiac metabolism. The effects could last 8 months.

Abstract:Objective：To investigate the changes of soluble cytokines and cell adhesion molecules in perioperative period of cardiopulmonary bypass（CPB） and to evaluate the effects of combined ultrafiltration on the expression of cytokines and cell adhesion molecules following CPB in children. Methods： Nineteen children with congenital heart disease were randomly divided into a control group（n = 10） and combined ultrafiltration（n = 9）. Plasma levels of interleukin-6， interleukin-8， ICAM-1 and VCAM-1 were assessed. Results： The plasma levels of IL-6， IL-8，ICAM-1， VCAM-1 increased to peak value at the end of CPB. The use of combined ultrafiltration could lower the plasma levels of IL-6， IL-8 and ICAM-1. There were significant differences between the two groups at the end of CPB（P < 0.05， P < 0.01， P < 0.05 respectively）. But the effect of combined ultrafiltration on the plasma level of VCAM-1 was not significant. Conclusion： After hypothermic CPB， cell adhesion molecules and cytokines are significantly increased. Using combined ultrafiltration during and after CPB can not only concentrate blood cell， alleviate the edima of tissues， but also lower the plasma levels of cytokines and adhesion molecules.

Abstract:Objective： To review the clinical experience in surgical treatment for Tetralogy of Fallot in adults over 15 years old. Methods：From October 1997 to January 2006，50 patients over 15 years old（15-57 years） underwent corrective repairment for Tetralogy of Fallot. Ventricular septal defects were repaired with Dacron patches in all patients. Forty-one cases were operated to widen the right ventricular outflow with patches and 9 cases underwent single repairment of right ventricular outflow. Results：One patient died from severe low cadiac output syndrome after operation. Heart functions and clinical manifestations were improved significantly in other patients. Conclusion：Proper surgical correction and careful perioperative management play an important role in the treatment of Tetralogy of Fallot in adults.

Abstract:Objective： To investigate the clinical efficacy of combined surgery of bronchial sleeve resection and other surgery procedures in 78 cases of central lung cancer. Methods： From June 1988 to June 2005，78 cases of central lung cancer underwent combined surgery procedure of bronchial sleeve resection and other surgery procedures， Brochial sleeve figuration and resection were performed in 57 cases， bronchial and arterial resection performed in 15 cases and carinal resection and reconstruction in 6 cases. Results：There was no operative death. 7 patients had operative complications. The rate of operative complication was 8.86 % （7／78）. The survial rate at 12，36，60 months and 10 years was 78.5％，59.5％，35.4％ and 17.7％ respectively. Conclusion：Bronchial sleeve resection， or comblined with arterial sleeve resection，can significantly preserve pulmonary function，improve the prognosis and increase the curative rate and long-term in patients with central lung cancer.