Abstract:Objective：To investigate the effect of prostaglandin E2 receptor EP3-Ⅲ on cell growth in human cholangiocarcinoma cells. Methods：Human cholangiocarcinoma cells HuccT1 was used in this experiment. The cells were treated with various concentrations of selective EP3 agonist sulprostone. The cell viability was detected by WST-8. The transcription of EP3-Ⅲ in HuccT1 was detected by RT-PCR. HEK293 cells were transiently transfected with EP3-Ⅲ by lipofectamine 2000TM. The cell viability was detected in the transfected cells by WST-8. Results：Sulprostone induced a concentration-dependent increase in cell viability in HuccT1 cells. RT-PCR analysis showed EP3-Ⅲ mRNA expression in HuccT1 cells. When HEK293 cells were transiently transfected with EP3-Ⅲ, the cell viability was increased after exposured to PGE2 10 μmol／L. Conclusion：These results demonstrate that EP3-Ⅲ is expressed in human cholangiocarcinoma cells，which may play an important role in cell growth.

Abstract:Objective：To investigate the effects of NMDA receptor on the long term potentiation（LTP） and c-fos expression induced by high frequency stimulus. in rat striatum in vitro. Methods：Both extracellular field potential recording and immunochemistry method were used in the study. Results：The results showed that LTP was inhibited by NMDA receptor antagonist MK-801（20 -滋mol／L） completely， and that activation of c-fos induced by tetanic stimulus in the same slices was inhibited. Conclusion：The present results demonstrated that NMDA receptor was involved in LTP and c-fos expression after tetanic stimulation.

Abstract:Objective：To observe the effect on humoral immunity induced by deglycosylated middle hepatitis B virus surface antigen（MHBs） in BALB／c mice. Methods：By the use of gene modification using PCR，Asn encoding codon AAT／AAC were replaced by Gln encoding codon CAG， so the N-glycosylation sites Asn-X-Thr／Ser（NXS／T） were replaced by Gln-X-Thr／Ser（QXS／T）. On the base of a DNA vaccine（pSW3891／MHBs／Adr） encoding MHBs, three new N-linked deglycosylation mutations were engineered：dG1，deglycosylation of Asn 4，located on the preS2; dG23，deglycosylation of Asn 59 and Asn 146，located on the HBs and dG123, deglycosylation of Asn4, Asn59 and Asn146. In vitro expressions following transient transfetion in 293T cell line were observed. Also the MHBs antibody titers were studied in BALB／c mice by different deglycosylated MHBs DNA vaccine. Results：HBsAg could be detected both in the supernatant and the cell lysate after transient transfection in 293T cell line with adr and dG23 DNA vaccine. It also could be detected in the cell lysate but not in the supernatant with dG1 and dG123. Mice immunized with adr、dG1 and dG23 showed strong anti-HBs response（ending point>1：102400）, but not with dG123（ending point<1：200）. Conclusion：The MHBs secretion in 293T cells was significantly influenced by the deglycosylation of Asn 4 in MHBs， but the antibody level induced by deglycosylated vaccine in the mice was not affected. The MHBs secretion and the antibody titers were slightly influenced by the deglycosylation of both Asn59 and Asn146，but they were completely impacted by the deglycosylation of Asn4，Asn59 and Asn146 simutaneously.

Abstract:Objective：To compare the effect of topical application of hydroxycamptothecin（HCPT） and mitomycin C（MMC） in preventing postlaminectomy epidural adhesion. Methods：Laminectomies were performed at L1 on 30 rats. Cotton pads soaked with 0.1 g／L HCPT, 0.1 g／L MMC or saline were applied to the operative sites for 5 min. Somatosensory evoked potential（SEP） was detected before or after administration. The effects of epidural adhesion after 4 weeks were observed with macroscopic observation，histopathological evaluation and MRI scanning. The count of fibroblasts and the area of epidural scar tissue were calculated. Results：Moderate to marked epidural adhesion was found in HCPT group，no obvious adhesion was formed in MMC group，while severe epidural adhesion formed in control group. SEP has no significant difference before and after administration. The count of fibroblasts and the area of epidural scar in HCPT and MMC group was obviously less than that in control group. Conclusion：Topical and transitory application of MMC and HCPT may be a successful method of preventing postlaminectomy epidural fibrosis.

Abstract:Objective：To investigate the effect of simvastain on the expression of BMP-2 and Smad1／5 in rats with ovariectomy-induced osteoporosis，and thus elucidate the mechanism of BMP-2 and Smad1／5 in nosogeny of osteoporosis and the regulating effect of simvastain. Methods：54 female SD rats were divided into three groups randomly：the experimental group，control group and model group .8 weeks after ovariectomy and sham-operation, simvastatin of 5 mg／（kg·d） were administered orally to the experimental group, and normal saline to the other two groups. Half of the rats were killed separately 4 weeks and 12 weeks after administration. Expression of BMP-2 and Smadl／5 in metaphysis of tibia was detected by immunochistochemistry. Results：① 4 weeks after administration，expression of BMP-2 was higher in experimental group and control group than that in model group（P < 0.05 or P < 0.01）; ②12 weeks after administration，expression of BMP-2 and Smadl／5 was higher in experimental group and control group than that in model group（P < 0.05 or P < 0.01）; ③Number of Smad1／5 positive cells and Smad1／5 gradation in experimental group was higher at 12 weeks after administration than 4 weeks（P < 0.01）. Conclusion：Hypo-expression of BMP-2 and Smadl／5 may be an important mechanism in nosogeny of primary osteoporosis. Simvastain can up-regulate BMP-2 expression in bone. Long-time administration of Simvastain can up-regulate Smadl／5 expression that may be an important mechanism of prevention and cure of osteoporosis.

Abstract:Objective：Ｔo estimate polymorphism in CagA protein systematically and analyze its characteristics by data miningusing SAS 9.0 soft package. Methods：CagA protein sequences were searched in protein databases in NCBI, swiss_prot／tremble and DDBJ. Data modification and program were performed with tools provided by SAS soft package. Data warehouse of CagA protein sequences were then built. These sequences were statistically analyzed by BioEdit7.0, SAS9.0 and student T test. Results:Redundant sequences were found and deleted. Complete CagA protein sequences of 97 strains and 3’ variable regions sequences of 268 Helicobacter pylori strains were obtained. Repeats in variable region were arranged in order in datasheets of SAS programs and observed clearly. The distinct position of variable region in CagA sequence was settled. Exact mean of number，polymorphisms and its distributing probability of EPIYA motif repeats were obtained. Interval sequences between two EPIYA motif repeats，knowledge of its kinds, characters and frequency were also obtained. The average of number of amino acids in variable regions is 115.76 ± 27.38 in 365 strains，which was the dominating causation that induced diversity of CagA proteins in length. EPIYA motifs repeated 3.28 ± 0.72 times in average，seven times in the maximum and one time in minimum in variable regions. EPIYA motifs have nine kinds of mutant，which account for 7.18% in all motifs. There are seven kinds of interval sequences in variable regions. “FPLKRHDKVDELIKVG” and “TIDDLGGP” in R3C and R4C motifs were characteristic sequences of Western-type stains. “FPLRRSAAVNDLSKVG” and “TIDFDEAN” in R4D motif were characteristic sequences of East Asian-type stains. Because of the diversity in the order and number of EPIYA-A，-B，-C，-D sites，there are 17 kinds different ABC-types or ABD-types in variable regions of CagA proteins. EPIYA motifs repeats and EPIYA-D sites in East Asian-type strains are significantly less，but EPIYA-A and EPIYA-B sites were more significant than that in Western-type strains. Conclusion: SAS soft package is effectively applied to analysis polymorphisms of variable region in CagA protein sequences. The characters of repeat sequences in variable region of CagA are elucidated on the whole. Their annotations become more reasonable，more systematic and more special than before. Because of the characteristics in polymorphism of cagA protein sequences, and their relationship with cytotoxicity，further research need to be done based on this study to uncover more molecular biological mechanisms.

Abstract:Objective：To investigated the inhibitory effect of B7-2 on pulmonary metastasis by B7-2 gene transfection into breast cancer cells. Methods：A pair of PCR inter primers for B7-2 KpnⅠ was designed based on the sequence registered in GenBank. XbaⅠand KpnⅠ sites were introduced into the 5′ ends of the primers，respectively. Adequate parts out of each end of B7-2 gene in genome were taken as a pair of outer primers. Mononuclear cells were isolated from human peripheral blood，incubated until clustering. Reverse transcript total RNA that isolated from those clustering cells. B7-2 gene was amplified with outer and inner primers with PCR. The PCR fragments were digested with the above two enzymes and then cloned into pcDNA3.1（+） vector to construct recombinant plasmid called B7-2pcDNA. The correct sequencing B7-2pcDNA clone was amplified and transtected into MD-MBA-231 cells. Another group of MD-MBA-231 cells were transfected with idling pcDNA3.1（+）. Both transfected groups and the control group were marked with FITC anti-human B7-2 antibody and then detected by flow cytometric analysis. MD-MBA-231／B7, MD-MBA-231／PCDNA3.1, and MD-MBA-231 cells were inoculated into BALB／c mice respectively. The influence of B7-2 on the tumorous metastasis was also observed. Results：The B7-2 recombinant gene was constructed and its length was 952 bp. The B7-2 coding sequence within it was 100% homology with B7-2 homo gene previously registered in GenBank. The flow cytometric analysis shew that B7-2pcDNA group was 36.77% and 51.88% higher than pcDNA3.1（+） and control ones in B7-2 express rate，respectively. The tumor growth rate of MD-MBA-231／B7 xenograft was significantly slower than MD-MBA-231 xenograft in BALB／c mice. MD-MBA-231／B7 cells in orthotopic implantation developed significantly less pulmonary metastasis than MD-MBA-231／pcDNA3.1 and MD-MBA-231 cells. Histologic findings showed that leukocytes were intensively infiltrated in both the MD-MBA-231／B7-2 tumors and its metastatic lesions，however，were scarcely observed in the lesions associated with MD-MBA-231 and MD-MBA-231／pcDNA3.1 cells. Conclusion：B7-2 may play an important role in inhibiting lymph node metastasis by the mechanism of enhanced immunogenicity，and that B7-2 gene transduction might be effective against lymph node metastases of breast cancer.

Abstract:Objective：To study sentinel lymph node biopsy（SLNB） in breast cancer patients by using 1% isosulfan blue or methylene blue. Method：206 patients were randomly divided into 2 groups，IB and MB，with injection different dye through peri-nipple and breast parenchyma. All patients undergoing modified radical mastectomy or radical mastectomy with axillary lymph nodes dissection（ALND）, and then the nodes in the ALND specimen were processed for histological examination. 8 female adult rabbits were randomly divided into 2 groups to study the dying time，number and fading time of the sentinel lymph nodes with different dyes. Results:In isosulfan blue group，SLNs were identified in 94 out of 102 patients，with a detection rate of 92.16%. The sensitivity of SLNB in this study was 92.31%，accuracy was 90.91%，false negative rate was 6.06%，false positive rate was 0.In methylene blue group，SLNs were identified in 97 out of 104 cases，with a detection rate of 93.27%. The sensitivity of SLNB in this study was 93.75%，accuracy was 91.35%, false negative rate was 6.25%，false positive rate was 0. No statistical significance was shown between isosulfan blue group and methylene blue group（P > 0.05）. In the rabbits group，the average staining time of the IB and MB group was 8.75s and 13.13s respectively. There are significant difference of the dying time，number and fading time of the sentinel lymph nodes between two groups（P = 0.000）. Conclusion：SLNB using methylene blue possesses the same characteristics of safety and feasibility as using isosulfan blue. Methylene blue is very cheap and can be widely used in the clinic.

Abstract:Objective：To investigate the expression of aquaporin-4 in human colorectal carcinoma tissue and analyze its clinical significance. Methods：Human colorectal carcinoma tissue was obtained from the First Affiliated Ｈospital of NJMU. The expressions of aquaporin-4 in human colorectal carcinoma tissue and paraneoplastic normal tissue were detected by Western Blot and reverse transcription PCR（RT-PCR） analysis, respectively. Results：The expression of aquaporin-4 in human colorectal carcinoma tissue was markedly higher than paraneoplastic tissue; moreover, the expression of aquaporin-4 in human colorectal carcinoma tissue was correlated with tumor stage and lymph node metastasis. Conclusion：The expression of aquaporin-4 in human colorectal carcinoma tissue was increased; aquaporin-4 might play an important role in tumor local invasion and metastasis.

Abstract:Objective：To investigate the function of promoter hypermethylation of secreted frizzled-related protein 2（SFRP2） gene in colorectal cancer. Methods：The promoter hypermethylation of SFRP2 gene in 44 sporadic colorectal cancer tissues and 20 adjacent normal mucosas of colorectal cancers were detected by fluorescence-based real-time PCR assay（MethyLight）， using 20 colorectal tissues of healthy volunteer as controls. And the relationship between hypermethylation of SFRP2 gene and clinicopathological features were analyzed. Results：SFRP2 gene hypermethylation was found in 81.8%（36／44）colorectal cancer tissues and 40%（8／20）adjacent normal mucosas of colorectal cancer. None of the normal colorectal mucosa tissues of volunteers showed any hypermethylated bands of SFRP2. Methylation in colorectal tumors was more frequent than in normal mucosa and adjacent tumor normal mucosa. Moreover，no significant associations were observed between SFRP2 hypermethylation and clinicopathological features such as sex，age， tumor stage，location and histological grade. Conclusion：Hypermethylation of SFRP2 gene is associated with the development of colorectal cancer. Hypermethylation of SFRP2 gene might serve as a biomarker for colorectal cancer screening.

Abstract:Objective:To observe the killing effect on pancreatic carcinoma(PC-3) cell lines by early growth response-1(Egr-1) promoter activating herpes simplex virus thymidine kinase(TK). Methods:Adenoviral vector of pAdEgr-1-TK was generated through homologous recombination in bacteria and was transfected to human PC-3.After exposured to γ-radiation by a 60Co source,hemi-quantitated by RT-PCR respectively to detect the expression of mRNA of TK in different radiation dose groups, in which 0 Gy act as control group. Then the cells were added prodrug ganciclovir(GCV),the survival rate was evaluated by MTT method. Results:After irradiation,transfected cell lines(0.08 ± 0.03) were killed by prodrug GCV at higher percentage significantly compared with control group(0.86 ± 0.11)(P < 0.001). Conclusion:It indicated that the Egr-1 promoter caused high expression of TK gene in cancer cells after exposured to 60Co-γ,which should significantly boost the killing effect on pancreatic cancer cell with the prodrug.

Abstract:Objective:To investigate the effects of Lipo-PGE1 on the microcirculation changes of intestine during acute intestinal venous congestion reperfusion in rats. Methods:30 SD rats were divided into three groups，the acute intestinal venous congestion reperfusion group（CR group）, the Lipo-PGE1 treatment group（Lipo-PGE1 group） and the sham operation group（SO group）. To induce intestinal venous congestion model, portal vein was clamped for 45 min, then unclamped for 60 min. In Lipo-PGE1 group, 0.2 μg Lipo-PGE1 was infused via veins 10 min before，just at clamping portal vein and 10 min after reperfusion respectively，while in CR group normal saline was used instead of Lipo-PGE1. The small intestinal mesenteric area was selected for observation of microcirculation. The numbers of capillary vessels with or without blood flow were recorded as reperfusion rate. Hemorrhagic spots around the capillary were also recorded. Intestinal species were obtained for histological examination after reperfusion for 60 min. The intestine injury was studied with Chiu’s Grade classification. Results:In CR group，the microcirculation reperfusion rates were much lower and the numbers of hemorrhagic spots around the capillary were much more than those in SO group. In Lipo-PGE1 group，however，the microcirculation reperfusion rates were much higher than in CR group，while no significant difference was found in hemorrhagic spots between the two groups. In CR group，optical microscopy showed that the intestine mucosa lesion was worse than that in SO group by Chiu’s Grade standard. But compared with CR group, it showed that intestine mucosa injury lesion was attenuated in Lipo-PGE1 group. Conclusion:Lipo-PGE1 could effectively protect microcirculation and attenuate the injury of intestine caused by acute intestinal venous congestion.

Abstract:Objective：To assess the correlation between the HBx gene integration in hepatocyte chromosome and HCC generation, investigate the difference of hepatitis B virus serum markers between patients with HCC and CHB, and to investigate the correlation between HBx gene integration and hepatitis B virus serum markers. Methods：Hepatocyte samples obtained from 19 patients with hepatocyte carcinoma or 14 subjects with chronic hepatitis B were prepared. The frequency of HBx gene integration was detected using biotin-marked HBx probe. Then the results were analyzed combining with the clinical data of hepatitis B virus serum markers.Results：①Among 19 patients with HCC， 13 subjects were found the signals of HBx gene hybridization in mitotic chromosome of tumour，which occupied 68.24% and was more than that in CHB（28.57%）（-字2 = 5.12，P < 0.05）. The HBx integration sites were 3.8 ± 1.36 in hepatocyte chromosome of HCC and 1.3 ± 0.36 in CHB，respectively（t = 3.5，P < 0.01）. ②The positive rate of HBeAg in serum was 80.0% in CHB，which is much higher than that in HCC（10.53%）. There was a significant difference（-字2 = 6.33，P < 0.01） between those two groups. ③There were no correlation between the frequency of HBx integration and the clinical hepatitis B virus serum marks either in HCC or in CHB. Conclusion：The frequency of HBx gene integration in HCC is higher than that in CHB. HBV reproducing in patients with CHB is more active than that with HCC.

Abstract:Objective：To investigate the serum amyloid A（SAA） protein levels possibly associated with the common cardiac and cerebral complications in patients with obstructive sleep apnea syndrome（OSAS）. Methods：Based on apnea hypopnea index（AHI） measured by polysomnography，62 patients with OSAS only were divided into mild OSAS group（n = 16），moderate OSAS group（n = 18） and severe OSAS group（n = 18）. Eighteen healthy subjects with matched age and BMI were recruited as control group. SAA specific monoclonal ELISA method was used to measure and compared morning SAA concentrations among different groups. In addition，correlation of SAA with AHI and minimal pulse oxygen saturation（miniSpO2） was analyzed in OSAS patients. Results：Analyzing on SAA concentrations demonstrated that compared with control group（9.19 ± 3.87 μg／ml）, SAA levels were significantly higher in mild OSAS group（13.49 ± 3.08 μg／ml，P < 0.05），in moderate OSAS group（18.73 ± 5.29 μg／ml, P < 0.01），and in severe OSAS group（38.56 ± 10.11 μg／ml，P < 0.01）. There was a significant difference in SAA levels among different groups. As the degrees of OSAS become more severe，SAA level became higher. Analysis indicated that SAA was positively correlated with AHI（r = 0.796，P < 0.001），but negatively correlated with miniSpO2（r = -0.631，P < 0.001）. Conclusion：SAA levels were significantly higher in OSAS patients and such an elevation turned to be more remarkable as degrees of OSAS become more severe. Elevated SAA level may contribute to some increased risk for cardiovascular and neuronal dysfunction in OSAS patients.

Abstract:Objective：To investigate the role of Lipopolysaccharide（LPS） on the proliferation of human renal tubular epithelial cell（HK-2） in vitro. Methods：HK-2 cells cultured were divided into 2 groups, negative control group and LPS treated group（1.0 ng／ml LPS）. The effect on the proliferation of HK-2 cells was detected by MTT technique. The morphological changes were observed under an invertedmicroscope. The expressions of interleukin-1（IL-1）, interleukin-10（IL-10） and tumor necrosis factor（TNF） were observed by indirect immunofluorescence assay. IL-1, IL-10 and TNF in the cultured supernatant were measured by ELESA. Results：LPS was able to promote the proliferation of HK-2 cells. Conclusion：LPS can promote the proliferation of human renal tubular epithelial cells and expression of inflammatory factor in vitro.

Abstract:Objective：Ｔo investigate the characteristics of cardiovascular autonomic nerve system（ANS） modulation in wrestling and judo athletes comparing with health untrained people. Methods：Thirty-two professional male wrestling and judo athletes（test group） and 32 health untrained male college students（control group） were tested by time domain, frequency domain and nonlinear analysis of short-term heart rate variability（HRV）. Results：By time domain method, the SDNN, RMSSD, SDSD and PNN50 in the athletes were significantly higher than those in health untrained people（P < 0.05）. By frequency domain method, the TP, LF and VLF were statistically higher in the athletes（P < 0.05） than control group. The LF／HF, HF, HFnu and LFnu were slightly higher in the athletes, but the differences didn’t have statistical significance. The results showed that there were significantly higher HRV in wrestling and judo athletes than in untrained people. Conclusion：Compared with the health untrained people，the level of parasympathetic and sympathetic tone were elevated in wrestling and judo athletes.

Abstract:Objective：To study the effects of necrosis factor alpha（TNF-α） and interleukin 6（IL-6）on liver regeneration in grafts using a novel model of partial liver transplantation in rats. Methods：Rats subjected to 50% liver resection（control） were compared with rats that underwent total hepatectomy and 50% partial liver transplantation. Livers were preserved at 4℃ for 45 min and 10 h respectively. The expression of the proliferative cell nuclear antigen（PCNA），TNF-α and IL-6 of graft in rats were evaluated by immuno histochemical method 1，2，4 d after operation，respectively. The mRNA of TNF-α and IL-6 was detected by reversed transcript polymerase chain reaction（RT-PCR）. And the relationship of graft regeneration and those cytokines were investigated.Results：Prolonged time of cold ischemia（10 h） was associated with a dramatic decrease of PCNA（P < 0.05）. TNF-α and IL-6 was significantly decreased in recipient rats compared with liver resection and 45 minutes of cold preservation group（P < 0.05）. The peak of liver regeneration delayed 24 h postoperation compared with liver resection group. Conclusion：TNF-α and IL-6 are significantly associated with the regenerative activation of the partial liver transplantation in early stage. Prolonged cold ischemia decreases the liver regeneration by expression of TNF-α and IL-6 production in graft.

Abstract:Objective：To investigate the effect of peroxisome proliferator-activated receptor γ（PPARγ） activator rosiglitazone in rat hepatic ischemia-reperfusion injury and its mechanism. Methods：The model of 70% warm ischemia-reperfusion injury was established in SD rats. Rats were divided randomly into 6 groups：control group, sham group, ischemia-reperfusion group, rosiglitazone group，2-chloro-5-nitrobenzanilide（GW9662） treatment group and rosiglitazone plus GW9662 treatment group. After reperfusion, AST and ALT levels in serum were detected. The liver tissue was removed for measurement of the apoptotic index by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 proteins in ischemic hepatocytes were detected by immunohistochemistry. Results：Compared with sham group, the apoptotic index of hepatocytes, expressions of Bax, Bcl-2 and Caspase-3 proteins in ischemia-reperfusion group and rosiglitazone group was greatly increased. Compared with ischemia-reperfusion group, the apoptosis index of hepatocytes, expressions of Bax and Caspase-3 in rosiglitazone group decreased, with Bcl-2 increased. GW9662 abolished the protective effect of rosiglitazone. GW9662 treated alone increased the apoptosis index of hepatocytes, Bax and Caspase-3, with the expression of Bcl-2 decreased. Conclusion：PPARγ activator rosiglitazone could protect against ischemia-reperfusion injury in rats，with its possible mechanism of upregulating the expression of Bcl-2, inhibiting the expression of Bax and Caspase-3, and prohibiting hepatocyte apoptosis.

Abstract:Objective：To obtain high expression level of HGF protein in vivo by HGF plasmid rapid injection through the tail vein. Methods：Systemic administration of different amount of naked plasmid containing HGF cDNA driven under cytomegalovirus promoter（pCMV-HGF） was injected rapidly into NOD／SCID mice through the tail vein, and the level of HGF protein in the circulation was detected by ELISA. The amount of HGF protein in liver tissue was measured through Western blot. Results：The amount of human HGF protein in the circulation was drastically increased after the injection of the HGF vector, and 20 -滋g／1.6 ml group increased highest. Ｆour hours after the injection of the pCMV-HGF vector, the HGF protein in the circulation of the mice was increased remarkably. Twelve hours later，the concentration of HGF protein reached the maximum that is about 10 ng／ml. Even 6 days after the injection，the HGF protein was still in a high level. The result of Western blot showed that the amount of HGF in liver tissue also increased after injection and reach the peak value at 8 h，and then decreased. Conclusion：Systemic administration of pCMV-HGF via rapid injection through the tail vein can promote high expression of HGF in vivo.

Abstract:Objective：To explore the effect and mechanisms of immune tolerance induced by injecting dendritic cells transfected with Fas ligand gene in allograft recipients. Methods：DCs were prepared from mouse bone marrow and genetically modified by Fas ligand. FasL-DC and LacZ-DC pooled from C57BL／6 donors were iv injected into BALB／c befor transplantation. The propliferative ability of T lymphocytes with the mixed lympiocyte reaction（MLR） was performed to further explore tolerance mechanisms. Results：The Fas ligand gene could be transfected into BMDC and expressed efficiently. The pretreatment of AdFasL modified DC greatly prolonged the survival of cardiac grafts in the recipients，extending survival from 10.0 ± 2.0 days（in LacZ-DC group） and 9.5 ± 1.5 days（in Day-7-DC group） to 21.5 ± 2.5 days. The result of MLR showed that allogeneic T lympiocyte cell proliferation was decreased significantly. Conclusion：The DC transfected with Fas ligand could induce apoptosis of the allogeneic reative T lymphocytes，and pretreatment of AdFasL modified DC could prolonged the survival of cardiac grafts in the recipients.

Abstract:Objective：To estimate the qualification, time, and technique of ventilator dependent patients weaning from the ventilation with the end-stage lung disease after lung transplant. Methods：A retrospective review of patient records and computerized database was performed. From September 2002 to June 2006, there were 6 cases of ventilator dependent recipients received lung transplant. Results：The 6 patiemts were weaned from the ventilator successfully in average 13.5d after lung transplatation. The follow-up time after operation was 1~4 years. The average life span was（２１.33 ± 3.48）month. Conclusion：The infection control，airway management, nutrition support, PGE1 utilizing, respiratory muscles exercise, and mental nursing are the important issue for weaning from the ventilator.

Abstract:Objective：To explore the surgical technics，early clinical results and the key point of perioperative management of single lung transplant in treating end-stage lung disease. Methods：From June，2006 to January，2007，4 cases of end-stage lung disease patients received right single lung transplantation. The first and the second case received the operation in the same day. Results：The first case was infected with bacillus pyocyaneus and amotile bacillus after operation，which resisted to almost all kinds of antibiotics. This case died of serious pulmonary infection 33 days postoperatively. The other 3 cases were discharged in 27 days，51 days and 60 days after operation respectively and all were in follow-up. The patients’ life quality, SaO2 and FEV1 improved significantly. Conclusion：Single lung transplantation is an effective treatment for end-stage lung disease. But the morbidity and mortality caused by infection and acute rejection after operation are very high. It was very important to enhance the perioperative management of lung transplantation.

Abstract:Objective:To prepare a new combined liver-kidney transplantation(LKT) model in rats and to investigate the protective role of the transplanted liver from transplanted kidney. Methods:In the donor operation,the liver and left kidney were flushed with 4℃ sodium lactate Ringer’s injected through portal vein and aorta,respectively. The superior hepatic vena cava was sutured. A patch of aorta attached to the donor kidney were anastomised to aorta between the donor and the recipient. The ureter and bile duct were anastomised using a simple inside bracket. The parameters of 3 groups were compared,such as survival time,renal function,pathology of the transplanted kidney. Results:The anhepatic duration of recipient was the same as that in Kanada’s method of liver transplantation with a relatively fewer hemodynamic changes and with a higher survival rate. The survival time of LKT recipients was much longer than kidney transplantation recipients(P < 0.05), but similar to liver transplantation recipients(P > 0.05). The concentration of blood urea nitrogen and creatine of combined transplantation group was greater than that of renal transplantation group(P < 0.05). The rejection of the latter was more serious than that of combined transplantation group. Conclusion:This technique of preparing simultaneous liver and kidney transplantation model in rats was feasible, and the simultaneously transplanted liver could protect kidney from rejection.

Abstract:Objective：To evaluate the efficacy of CEUS（contrast enhanced ultrasonography） in accessing immediate curative effects of malignant liver tumor after radiofrequency or microwave therapy. Methods：Fifty-six lesions confirmed by contrast CT, CEUS or biopsy were observed. No enhancement through all periods and normalized serologic indexes were considered as the standard of complete treatment. The phenomena such as residual lesions enhanced through the arterial period, diminished through venous and delayed phase, which similar to that before therapy, was considered as standard of incomplete treatment. Consecutive therapy was applied to those regions until that reached complete treatment standard. Results：CEUS can dynamically show the microcirculation characteristics of therapy regions and different appearance of residual lesions. Conclusion：CEUS can show appearances of therapy regions as well as residual lesions，which should help to make supplementary therapeutic plans.

Abstract:Objective：To investigate the usefulness of immunohistochemical expression and immunolocalization of a panel of papillary thyroid carcinoma markers including HBME-1，CK19 and Galectin-3. Methods：By immunochemical EnVision method, the expression of HBME-1，CK19 and Galectin-3 were detected in 55 cases of papillary thyroid carcinomas，24 follicular adenomas, 20 multinodular goiters and 10 Hashimoto’s thyroiditis. Results：The rates of immuno-positive staining of HBME-1，CK19 and Galectin-3 in papillary thyroid carcinoma were 96.4%，98.2% and 94.5%, respectively, which were significantly higher than that of benign lesions（20.4%, 20.4% and 22.2%, respectively）. No significant relationship was found among the expressions of HBME-1, CK19 and Galectin-3. The sensibility, specificity，positive predictive value, negative predictive value and accuracy rate of using HBME-1 combined with CK19, Galectin-3 in differentiation benign from papillary thyroid carcinoma were 100%, 96.9%, 98.8%, 98%, 100% respectively. Conclusion：An immunohistochemical panel consisting of HBME-1, CK19 and Galectin-3 can make a correct differential diagnosis between papillary thyroid carcinoma and its benign mimics.