Abstract:Objective: This article provides an overview of characteristics of phospholipids, the characteristics and influential factors of liposome and microemulsion as carriers for skin delivery of drugs, and the latest advances of the phospholipids carriers in transdermal delivery systems. The perspective is that phospholipids carriers may be capable of a wide range of applications in the transdermal delivery system.

Abstract:Objective: To test whether IL-1 RI/My088-TIR mimic AS-1 can work as a new compound that targeted at blocking MyD88-dependent signaling pathway, we investigated the physical structure and biological function of AS-1. Methods:The crystallographic structure of AS-1 was examined by 1H nuclear magnetic resonance. The toxicity of AS-1 was measured with Methyl thiazolyl tetrazolium(MTT) assay. The effect of AS-1 on phosphorylation state of p38 MAPK and IRAK-1 was observed with Western blot. Results:The crystallographic details of AS-1 demonstrated that it was a tri-peptide sequence[(F/Y)-(V/L/I)-(P/G)] of the IL-1RⅠ-TIR domain BB-loop. No toxicity of AS-1 was shown to HEK 293A cells. The phosphorylation of p38 MAPK, induced by IL-1β significantly increased from those in the control group. AS-1 significantly reduced the phosphorylation of p38 MAPK induced by IL-1β. IL-1β increased the phosphorylation of IRAK-1 significantly, which was prevented by AS-1. Conclusion:AS-1 is a competitive mimic between IL-1RⅠ-TIR and MyD88-TIR domain, which most likely interferes with MyD88-dependent signaling pathway.

Abstract:Objective: To investigate the mechanisms that Simvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA) reductase inhibitor, plays an important role in primary prevention of atherosclerosis independently of its lipid-lowering effect in Apolipoprotein E-deficient mice in the early stage of atherosclerosis. Methods: Twenty-four 6-week old male apoE-deficient mice were randomly divided into two groups:control group(normal saline) and treatment group[simvastatin(5 mg/(kg·d))]. Simvastatin was administered to treatment group mice by gavage and the same volume of normal saline was administered to control group mice by the same method for 2 or 4 weeks.Total cholesterol(TC), super-oxide dismutase(SOD), malondialdehyde(MDA) and serum nitric oxide(NO) were measured by bio-chemical analysis. Results: There was no significant difference in serum TC between control and treatment groups. Compared with the control’s, the effects of simvastatin were more significant in decreasing serum MDA level(P < 0.01 vs control’s at 2-week; P < 0.006 vs control’s at 4-week), increasing serum SOD level(P < 0.03 vs control’s at 2-week; P < 0.003 vs control’s at 4-week) and NO level(P < 0.01 control’s at 2-week; P < 0.001 vs control’s at 4-week) either at 2 or 4 weeks. Conclusion: Simvastatin attenuates oxidative stress and protects endothelial function by the mechanisms of decreasing serum MDA level, increasing serum SOD level and NO level , which were inconsistent with its cholesterol-lowering effect. It may play an important role in primary(if not all) prevention of atherosclerosis and might be independent of lipid-regulation mechanism.

Abstract:Objective: This investigation was designed to stratify patients with acute chest pain based on their symptoms, electrocardiogram (ECG), cardiac injury markers and the number of accompanying traditional risk factors(smoking, obesity, hyperlipemia, hypertension, diabetes), and to assess the effect of the above factors to obtain a risk stratification for patients with chest pain. Methods: We identified 139 patients with acute chest pain, including 45 myocardiac infarction patients, 65 unstable angina patients and 29 chest pain patients without identified acute coronary syndrome(ACS) admitted to our Coronary Heart Center during December 2004 to February 2005. All patients accepted coronary angiography. All data was collected using questionnaires. Based on reported symptom, electrocardiogram (ECG), cardiac injury markers and the number of the accompanying traditional risk factors, we stratified all patients into four groups: Group 1, patients with acute chest pain, ECG changes and abnormal cardiac injury biomarkers. Group 2, patients with acute chest pain and ECG changes(without abnormal cardiac injury biomarkers). Group 3, patients with acute chest pain, normal ECG, normal cardiac injury biomarkers and >2 traditional risk factors. Group 4, patients with acute chest pain, normal ECG and normal cardiac injury biomarkers, but only≤2 traditional risk factors. From this data we examined the difference of ACS incidence in the four groups. Results:After stratification the ACS incidence of the grouped patients in turn was 100%, 84%, 69.6% and 53.3%. The combination of early phase ECG and cardiac injury markers identified 70.9% patients with ACS(the specificity being 90.7%). The mortality of group 3 was higher compared with group 4(69.6% vs 53.3%), however the P value was more than 0.05 and didn’t show significant statistical difference. The correlation analysis found the number of the traditional risk factors had a significant positive correlation (r = 0.202, P = 0.044) with the number of stenosis being more than 50% of the artery diameter. Multiple linear regression showed the hypertension had a significant correlation with the number of the diseased regions(P = 0.014). Conclusions:The risk stratification based on the symptom, ECG, cardiac injury markers and accompanying traditional risk factors is both important and available in practice. It is unsuitable for patients with a normal ECG and cardiac injury markers to differentiate ACS from non-cardiac chest pain relying only on the number of the accompanying traditional risk factors. However we found the number of the risk factors can indicate the disease severity.

Abstract:Objective: To investigate the histomorphological change in auto-extremity artery following transplantation. Methods: 50 New Zealand rabbits were randomly divided into 5 groups(postoperative 1 d, 3 d, 7 d, 14 d, 56 d, n = 10). Femoral artery was harvested and end-to-side anastomosed with carotid in order to build the auto-extremity arterial graft animal model. On the postoperative 1st, 3rd, 7th,14th and 56th days, grafts for morphometric analysis under the Image analysis system were obtained; and electron microscope was scanned to observe endothelial cells. In addition, Immunostaining of sections were performed with the mouse monoclonal antibody of the α-smooth muscle isoform of actin and proliferating cell nuclear antigen antibody. Results: Overall patency rate for all conduits was 86%.The intimal hyperplasia was first observed in the 7th day group, and continued to increase in the 56th day group(183.21±111.74)μm, P < 0.01. Additionally, the luminal narrowed(32.43±18.28)% in the 56th day group. Smooth muscle cells were the mainly hyperplastic components. The most active proliferation of cells was detected in the 14th day group, where the extracellular matrix gradually deposited in the intima. Conclusion: Moderate intimal hyperplasia occurred in arterial conduits and vascular structure experienced constrictive remodeling after auto-transplantation.

Abstract:Objective: To study the inhibition of proliferation of breast cancer by small interfering RNA(siRNA) targeting human prolactin(hPRLR) and the underlying mechanisms. Methods:The siRNA targeting hPRLR was chemically synthesized and transfected into MCF-7 cells, the expression of hPRLR was analyzed by real-time quantitive PCR, cell growth inhibition was measured with MTT assay, cell cycle of the transfected cells was examined by flow cytometry, meanwhile, expression of cyclin D1 was tested by semi-quantitative RT-PCR. Results:24 h after transfection with 100 nmol/L siRNA-PRLR, the expression of hPRLR mRNA was suppressed by 65%, cells in G1 phase increased, but cells in S phase decreased. Down regulated hPRLR expression exhibited significant inhibition in cell proliferation. And the expression of cyclin D1 was down regulated. Conclusion:The results indicate that siRNA-hPRLR is a useful tool for silencing hPRLR expression and inhibiting cell proliferation in breast cancer MCF-7 cell line, and it may be a possible new approach for breast cancer gene therapy.

Abstract:Objective: To evaluate the effects of different concentrations of phosphate on calcium deposition and osteocalcin level in cultured bovine aortic smooth muscle cell, investigate the mechanism of hyperphosphatemia to evoke calcification of vascular smooth muscle cell and observe the effects of phosphonoformic acid(PFA) in different concentrations on vascular calcification. Methods: The bovine aortic smooth muscle cells (BASMC) were cultured.Calcium deposition and the expression of osteocalcin of BASMC in different concentrations of phosphate (1.5 mmol/L and 2.0 mmol/L) and PFA were determined by o-cresolphthalein complexone and radioimmunity methods, respectively. Osteocalcin mRNA expressions were determined by RT-PCR. Results: After six or nine days of BASMC cultured, the calcium deposition in Pi 2.0 mmol/L group was more than that in Pi 1.5 mmol/L group[(77.187±11.692)μg/(mg·protein) vs(25.768±1.750)μg/(mg·protein), P < 0.01 and(125.399±16.677)μg/(mg·protein) vs(29.046±2.635)μg/(mg·protein), P < 0.01 respectively]. The calcium deposition was dependent on time and dosage of phosphate treatment. After 72 h culture the osteocalcin in Pi 2.0 mmol/L group was more than that in Pi 1.5 mmol/L group[in supernatant,(1.503±10-2±2.601×10-3)ng/(μg· protein) vs(2.981×10-3±8.382×10-4)ng/(μg·protein), P < 0.001], the same was found in osteocalcin mRNA expression[OC/GAPDH,(1.906±0.132) vs(0.748±0.037)，P < 0.001]. Compared to Pi 1.5mmol/L group,bovine smooth muscle cells(BSMC) cultured in media containing Pi 2.0 mmol/L phosphate levels increased calcium deposition[On day 6,(77.187±11.692)μg/(mg·protein) vs(25.768±1.750)μg/(mg·protein), P < 0.001]. Elevated phosphate treatment of BSMCs also enhanced the expression of the osteoblastic differentiation marker osteocalcin[On day 3, Pi 2.0 mmol/L group vs Pi 1.5mmol/L group,(1.503×10-2±2.601×10-3)ng/(μg·protein) vs(2.981×10-3±8.382×10-4)ng/(μg·protein), P < 0.001]. PFA decreased ciacium deposition and osteocalcin expres-sion statistically[Pi 2.0 mmol/L+PFA1.0 mmol/L group vs Pi 2.0mmol/L group , ciacium deposition, (37.729±5.899)μg/(mg· protein) vs (77.187±11.692)μg/(mg·protein), P < 0.001]; Osteocalcin in supernatant, (4.529±10-3±1.250×10-3)ng/(μg·protein) vs(1.503×10-2±2.601×10-3) ng/(μg·protein), P < 0.001; osteocalcin mRNA expression, OC/GAPDH, (0.642±0.092) vs (1.89±0.165), P < 0.01]. Conclusion: Hyperphosphate may directly promote calcium deposition and the osteocalcin expression of BASMCs. It may be a new explanation for the phenomenon of vascular calcification in hyperphosphatemic conditions. Hyperphosphatemia is an independent factor to stimulate vascular calcification. PFA can inhibit calcium deposition and osteocalcin expression induced by elevated phosphate.PFA may be a new medicine to treat vascular calcification induced by elevated phosphate.

Abstract:Objective:To investigate correlation between C-reactive protein(CRP) and gestational diabetes mellitus(GDM). Methods:Twenty-five GDM women were served as study group, and thirty normal pregnant women were selected as control group. The serum FPG,2hPG, HbA1c and CRP levels and the leukocyte count were detected in the two groups, in order to observe the relationship between gestational diabetes mellitus and inflammatory markers. Results:The age and gestational week did not show difference in the two groups(P > 0.05). But there was a significant difference in body mass index(BMI) between the GDM group and the control group(P < 0.05). The serum FPG, 2hPG, HbA1c and CRP levels and the leukocyte count in the GDM group were higher than those in the control group, and the difference was significant(P < 0.05). There was positive correlation between serum C-reactive protein value and FPG, 2hPG, HbA1c serum levels or the leukocyte count in GDM group. But in the control group there was no correlation between them. Conclusion:The results suggest that there is correlation between C-reactive protein and gestational diabetes mellitus, and inflamma-tion may play an important role in the development of gestational diabetes mellitus.

Abstract:Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function, atrio-ventricular node function and hemodynamics. Methods:Thirty-two Angora’s rabbits were selected and randomly divided into four groups. U1 group:urapidil 0.25 mg/kg; U2 group:urapidil 0.5 mg/kg; N1 group:nicardipine 10 μg/kg; N2 group: nicardipine 20 μg/kg. All these medicine were administrated within 30 seconds. Measurements were taken before and after the administration of urapidil or nicardipine for the follow-ing data: mean blood pressure(MAP), heart rate(HR), sino-atrial conduction time(SACT), maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT) , index of sinus node recovery time(SNRTI), Wenckebach A-V conduction frequency (WB), and P-R interval. Results:Significant MAP and HR changes were identified in all of the four groups before and after adminis-tration of both urapidil and nicardipine. No significant changes could be found in the rest of the parameters. Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P < 0.01); the MAP decreased(P < 0.01) and the HR increased drastically(P < 0.01). Conclusions:Neither urapidil(0.25 mg/kg, 0.5 mg/kg) nor nicardipine(10μg/kg, 20μg/kg) has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function. Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.

Abstract:Objective:To assess the capability of magnetic resonance imaging(MRI) in evaluating the cardiac structures and function in the hypertrophic cardiomyopathy(HCM). Methods:Fourteen healthy volunteers and eighteen cases with HCM verified by history, clinical presentation, electrocardiogram and echocardiography(ECG) were performed with MRI. The myocardial thickness of interventricular septum at the basal segment and that of posterolateral free wall of the left ventricle(LV) were measured. Some indexes for evaluating cardiac function were measured using ARGUS auto-quantitative program. Results:The myocardial thickness of septum at the basal segment had significant difference between the HCM patients and the healthy volunteers. There was no significant difference between MRI and ECG in examining end-diastolic volume, ejection fraction of the LV. Conclusion:MRI can fully provide more information on the abnormalities of cardiac anatomy and function; thus, it is of great value in clinical application.

Abstract:Objective:To investigate the correlation between obstetrical complications and depressive disorder. Methods:Depressive disorder probands and their adult sibling were diagnosed using CCMD-3 criteria. Obstetrical data from maternal reports were scored, applying published scales that take into account number and severity of complication. Results:The scores of obstetric complication and prenatal complications and low birth weight were significantly worse in probands than siblings without depressive disorders. Conclusion:Results suggest obstetric complications are etiologically significant in depressive disorder.

Abstract:Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/9cases), PR(4/9cases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.

Abstract:Objective: To examine the expression patterns of p63 in tissues of particular keratinocyte original hyperproliferate diseases and variety cell types for determining if P63 is the marker of proliferative potential keratinocytes. Methods: P63 protein was detected and analyzed by immunoreactivity method and Western blot in biopsy specimens of keratinocyte original disorders including squamous cell carcinomas SCC, basal cell carcinomas BCC, Bowen’s disease and other tissues or cells, such as psoriasis vulgaris, normal skin tissues, primary cultured keratinocytes, immortal HaCaT cells, and epidermoid carcinoma cells A431. Results: P63 protein was expressed in the nuclei of basal and suprabasal layer of the epidermis, germinative cells of sebaceous glands in normal epidermal. P63 was strongly and diffusely detected in the majority of tumor cells in BCC and poorly-differentiated SCC. In Bowen’s disease, p63 expresses are remarkable in all cell layers. In the psoriasis plaque epidermal, p63 expressed mainly in basal cells and part of spinous cells. P63 expressed more strongly in primary cultured keratinocytes than in A431 cells or HaCaT cells. Conclusion: P63 is a nuclei marker of undifferentiated keratinocytes with the proliferative potential and may disrupt the terminal differentiation. The overexpression of p63 reflects immaturity of the tumor cells. The immunohistochemical staining of p63 may be useful for investigating the origin and differentiation of tumor cells.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ ｒｅｃｅｐｔｏｒ（ＥＧＦＲ） ａｎｄ ｔｈｅ ＥＧＦＲ ｓｉｇｎａｌ ｔｒａｎｓｄｕｃｔｉｏｎ ｐａｔｈｗａｙ ｉｎ ｈｕｍａｎ ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ（ＨＣＣ） ｃｅｌｌｓ， ａｎｄ ｔｏ ｅｘｐｌｏｒｅ ｔｈｅ ｍｏｌｅｃｕｌａｒ ｍｅｃｈａｎｉｓｍ ｏｆ ＥＧＦＲ ｐａｔｈｗａｙ ｍｅｄｉａｔｉｎｇ ｔｈｅ ｐｒｏｌｉｆｅｒａｔｉｏｎ ｏｆ ＨｕＨ７ ｃｅｌｌ ｌｉｎｅ． Ｍｅｔｈｏｄｓ：Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＥＧＦＲ ｉｎ ＨＣＣ ｔｉｓｓｕｅｓ ｗｅｒｅ ｅｖａｌｕａｔｅｄ ｂｙ ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｓｔｒｙ（ＩＨＣ）． ＨｕＨ７ ｃｅｌｌｓ ｗｅｒｅ ｔｒｅａｔｅｄ ｗｉｔｈ ＥＧＦ ａｎｄ ＡＧ１４７８． Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ ｗａｓ ｅｍｐｌｏｙｅｄ ｔｏ ｄｅｔｅｃｔ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎｓ ｏｆ ＥＧＦＲ， ｐ-ＥＧＦＲ ａｎｄ ｐ-ＥＲＫ ｉｎ ＨｕＨ７ ｃｅｌｌｓ， ａｎｄ ｔｈｅ ｃｅｌｌ ｖｉａｂｉｌｉｔｙ ｗａｓ ｄｅｔｅｒｍｉｎｅｄ ｂｙ ｔｈｅ ｃｅｌｌ ｐｒｏｌｉｆｅｒａｔｉｏｎ ｒｅａｇｅｎｔ ＣＣＫ-８． Ｒｅｓｕｌｔｓ：Ａｍｏｎｇ １７ ｃａｓｅｓ ｏｆ ＨＣＣ ｔｉｓｓｕｅｓ，ｔｈｅ ｐｏｓｉｔｉｖｅ ｒａｔｅｓ ｏｆ ＥＧＦＲ ｄｅｔｅｃｔｅｄ ｗｅｒｅ １００％，ｗｈｉｌｅ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＥＧＦＲ ｉｎ ＨｕＨ７ ｗａｓ ｍｏｒｅ ｓｔｏｎｇｅｒ ｔｈａｎ ｔｈａｔ ｉｎ ＨＬ-７７０２． Ｅｘｏｇｅｎｏｕｓ ＥＧＦ（１０ μｇ／Ｌ） ｅｎｈａｎｃｅｄ ｔｈｅ ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ ｏｆ ＥＲＫ ａｎｄ ＥＧＦＲ， ａｎｄ ａｌｓｏ ｔｈｅ ｇｒｏｗｔｈ ｒａｔｅ ｏｆ ＨｕＨ７ ｃｅｌｌｓ ａｔ ２４ ｈ． Ｂｕｔ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ＥＧＦ ｃｏｕｌｄ ｂｅ ｔｏｔａｌｌｙ ｉｎｔｅｒｒｕｐｔｅｄ ｂｙ ＡＧ１４７８（２０ μｍｏｌ／Ｌ）． Ｃｏｎｃｌｕｓｉｏｎ：Ｏｕｒ ｆｉｎｄｉｎｇｓ ｓｕｇｇｅｓｔｅｄ ｔｈａｔ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＥＧＦＲ ｓｈｏｕｌｄ ｂｅ ｐｏｓｉｔｉｖｅ ｉｎｔｅｎｓｉｖｅｌｙ ｉｎ ｈｕｍａｎ ＨＣＣ ｃｅｌｌ ｌｉｎｅ ＨｕＨ７， ａｎｄ ｔｈｅ ａｃｔｉｖａｔｉｏｎ ｏｆ ＥＧＦＲ ｄｅｐｅｎｄ ｕｐｏｎ ｔｈｅ ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ ｏｆ ＥＧＦＲ． ＥＧＦＲ-ＥＲＫ／ＭＡＰＫ ｓｉｇｎａｌ ｔｒａｎｓｄｕｃｔｉｏｎ ｐａｔｈｗａｙ ｍｉｇｈｔ ｐｌａｙ ａ ｋｅｙ ｒｏｌｅ ｉｎ ＨｕＨ７ ｃｅｌｌｓ， ｗｈｉｃｈ ｒｅｇｕｌａｔｅｄ ｔｈｅ ｄｅｖｅｌｏｐｍｅｎｔ ａｎｄ ｐｒｏｇｒｅｓｓｉｏｎ ｏｆ ｈｕｍａｎ ＨＣＣ ｃｅｌｌｓ．

Abstract:Objective：To construct the podocin immunofluorence expression vector and observe its effects of transfection on CD2AP distribution in HEK293 cells. Methods：The pGEMT-easy vector containing the full-length cDNA encoding the human podocin was cloned and digested with BamHI and XhoI. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plasmids were transfected into HEK293 cells，and its effect on CD2AP distribution were observed by immunofluorescence. The interaction between podocin and CD2AP was detected by immunoprecipitation. Results：①The pEGFP-NPHS2 expression vector was successfully constructed and podocin exclusively located on HEK293 cell membrane.② After podocin transfection, CD2AP redistributed from perinucleus to cytoplasm in HEK293. Conclusion：Podocin can recruit CD2AP to redistribute from perinucleus to cytoplasm in HEK293 cells.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｃｅｌｌ ｇｒｏｗｔｈ ｉｎｈｉｂｉｔｉｏｎ ｉｎｄｕｃｅｄ ｂｙ ｓｅｌｅｃｔｉｖｅ ｃｙｃｌｏｏｘｙｇｅｎａｓｅ-２（ＣＯＸ-２） ｉｎｈｉｂｉｔｏｒ ｃｅｌｅｃｏｘｉｂ ａｎｄ ｉｔｓ ｍｏｌｅｃｕｌａｒ ｍｅｃｈａｎｉｓｍｓ． Ｍｅｔｈｏｄｓ：Ｃｅｌｌ ｇｒｏｗｔｈ ｒａｔｅｓ ｗｅｒｅ ａｓｓｅｓｓｅｄ ｂｙ ＷＳＴ-１ ａｓｓａｙ． Ａｐｏｐｔｏｓｉｓ ｗａｓ ｅｘａｍｉｎｅｄ ｂｙ ｆｌｏｗ ｃｙｔｏｍｅｔｒｙ． Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＣＯＸ-２， ｃａｓｐａｓｅ-３ ａｎｄ Ａｋｔ ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ ｗｅｒｅ ｅｘａｍｉｎｅｄ ｂｙ ｗｅｓｔｅｒｎ ｂｌｏｔ ａｎａｌｙｓｉｓ． Ｒｅｓｕｌｔｓ：Ｃｅｌｌｓ ｗｅｒｅ ｔｒｅａｔｅｄ ｗｉｔｈ ｉｎｃｒｅａｓｉｎｇ ｃｏｎｃｅｎｔｒａｔｉｏｎｓ ｏｆ ｃｅｌｅｃｏｘｉｂ（０-５０μｍｏｌ／Ｌ） ｆｏｒ ２４ ｈｏｕｒｓ，ａｎｄ ｔｈｅ ｃｅｌｌ ｇｒｏｗｔｈ ｒａｔｅ ｗａｓ １００％，８１．１５％，６６．７２％，５４．９３％ ａｎｄ １１．４１％， ｒｅｓｐｅｃｔｉｖｅｌｙ． Ｔｈｅ ａｐｏｐｔｏｃｉｃ ｒａｔｉｏｎ ｏｆ ５０ μｍｏｌ／Ｌ ｇｒｏｕｐ ｗａｓ ３０．７２％． Ｔｈｅ ｒｅｍａｒｋａｂｌｅ ａｃｔｉｖａｔｉｏｎ ｏｆ ｃａｓｐａｓｅ-３ ａｎｄ ｓｉｇｎｉｆｉｃａｎｔ ｒｅｄｕｃｔｉｏｎ ｏｆ Ａｋｔ（Ｔｈｒ３０８） ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ ｃｏｕｌｄ ｂｅ ｏｂｓｅｒｖｅｄ ｂｙ ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｅ ｃｅｌｅｃｏｘｉｂ ｉｎｈｉｂｉｔｅｄ ｐｒｏｌｉｆｅｒａｔｉｏｎ ａｎｄ ｉｎｄｕｃｅｄ ａｐｏｐｔｏｓｉｓ ｏｆ ｔｈｅ ｖａｓｃｕｌａｒ ｓｍｏｏｔｈ ｍｕｓｃｌｅ ｃｅｌｌｓ ｉｎ ａ ｄｏｓｅ ｄｅｐｅｎｄｅｎｔ ｍａｎｎｅｒ， ｗｈｉｃｈ ｉｓ ｐａｒｔｌｙ ｒｅｌａｔｅｄ ｔｏ ｔｈｅ ｍｅｃｈａｎｉｓｍ ｏｆ Ａｋｔ／ｃａｓｐａｓｅ ｐａｔｈｗａｙ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｓｅｌｅｃｔｉｖｅ ＣＯＸ-２ ｉｎｈｉｂｉｔｏｒ ｃｅｌｅｃｏｘｉｂ ｏｎ ｃｅｌｌ ｃｙｃｌｅ ａｒｒｅｓｔ ｉｎ Ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ ｃｅｌｌｓ． Ｍｅｔｈｏｄｓ：Ｔｈｒｅｅ ｄｉｆｆｅｒｅｎｔ ｈｕｍａｎ ｈｅｐａｔｏｍａ ｃｅｌｌ ｌｉｎｅｓ ＨＵＨ-７， Ｈｅｐ３Ｂ ａｎｄ ＨｅｐＧ２， ｗｅｒｅ ｕｓｅｄ ｉｎ ｔｈｉｓ ｅｘｐｅｒｉｍｅｎｔ． Ｔｈｅ ｃｅｌｌｓ ｗｅｒｅ ｔｒｅａｔｅｄ ｗｉｔｈ ｖａｒｉｏｕｓ ｄｅｇｒｅｅｓ ｏｆ ｃｅｌｅｃｏｘｉｂ ｆｏｒ ｄｉｆｆｅｒｅｎｔ ｔｉｍｅ． Ｔｈｅ ｃｅｌｌ ｖｉａｂｉｌｉｔｙ ｗａｓ ｄｅｔｅｃｔｅｄ ｂｙ ＷＳＴ-８． Ｔｈｅ ｃｅｌｌ ｃｙｃｌｅ ｏｆ ｔｈｅ ｈｅｐａｔｏｍａ ｃｅｌｌｓ ｗａｓ ａｎａｌｙｚｅｄ ｂｙ ｆｌｏｗ ｃｙｔｏｍｅｔｒｙ． Ｒｅｓｕｌｔｓ：Ｃｅｌｅｃｏｘｉｂ ｉｎｄｕｃｅｄ ａ ｓｉｇｎｉｆｉｃａｎｔ ｄｅｃｒｅａｓｅ ｉｎ ｃｅｌｌ ｖｉａｂｉｌｉｔｙ ｉｎ ｔｈｅｓｅ ｔｈｒｅｅ ｈｅｐａｔｏｍａ ｃｅｌｌ ｌｉｎｅｓ． Ｆｌｏｗ ｃｙｔｏｍｅｔｒｙ ｓｈｏｗｅｄ ａ ｃｅｌｌ ｃｙｃｌｅ ａｒｒｅｓｔ ｉｎ Ｇ０／Ｇ１， ａｎｄ ａ ｗｅａｋ ａｒｒｅｓｔ ｉｎ Ｇ２／Ｍ ｃｈｅｃｋｐｏｉｎｔ， ｗｈｅｎ ｔｈｅ ｃｅｌｌｓ ｗｅｒｅ ｅｘｐｏｓｅｄ ｔｏ ｃｅｌｅｃｏｘｉｂ ２５ μｍｏｌ／Ｌ ｆｏｒ ２４ ｈ． Ａｎｄ ｔｈｉｓ ｅｆｆｅｃｔ ｓｅｅｍｓ ｔｏ ｂｅ ｓｔｒｏｎｇｅｒ ｉｎ ４８ ｈ． Ｃｏｎｃｌｕｓｉｏｎ：Ｃｅｌｅｃｏｘｉｂ ｉｎｄｕｃｅ Ｇ０／Ｇ１ ａｎｄ Ｇ２／Ｍ ｃｅｌｌ ｃｙｃｌｅ ａｒｒｅｓｔ ｉｎ ｈｕｍａｎ ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃｅｌｌｓ， ｗｈｉｃｈ ｍａｙ ｐｌａｙ ａｎ ｉｍｐｏｒｔａｎｔ ｒｏｌｅ ｉｎ ｈｅｐａｔｏｍａ ｃｅｌｌ ｇｒｏｗｔｈ ｉｎｈｉｂｉｔｉｏｎ．

Abstract:Objective：To investigate the time correlation with the activation of molecule targets in Toll Like Receptor 4（TLR4） signaling in mice myocardium activated by lipopolysaccharide（LPS） and its significance. Methods：Male C57BL／6J mice were randomly divided into LPS group（intraperitoneal injection with LPS 10 mg／kg） and saline control group. The expression of molecule targets in TLR4 signaling parthway were detected at 5 min，10 min，30 min，1 h，2 h，6 h，12 h，24 h（n = 3） after treatment by Ｗestern blotting. Resultｓ：The expression of TLR4-MyD88-IκBα signaling is activated 5 min after injection, reached the peak at 2 h, then decreased（P < 0.05）. The expression of MAPKs is activated 5 min after injection, reached the peak at 6 h, then decreased（P < 0.05）. The expression of Akt is activated 10 min after injection, reached the peak at 6 h, then decreased（P < 0.05）. Conclusion： There is a close correlation between the activation of TLR4 signaling and the time of LPS treatment in mice myocardium .

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｏｘｉｃｉｔｙ ｏｆ ｄｅｏｘｙｐｏｄｏｐｈｙｌｌｏｔｏｘｉｎ（ＤＯＰ） ａｇａｉｎｓｔ ３ｒｄ ｉｎｓｔａｒ ｌａｒｖａｅ ｏｆ Ｐｉｅｒｉｓ ｒａｐａｅ Ｌ． ａｎｄ ｉｔｓ ｅｆｆｅｃｔｓ ｏｎ ａｃｔｉｖｉｔｉｅｓ ｏｆ ＡＣｈＥ ａｎｄ ＡＴＰａｓｅ， ａｎｄ ｔｏ ｓｔｕｄｙ ｉｔｓ ｉｎｓｅｃｔｉｃｉｄａｌ ｍｅｃｈａｎｉｓｍ． Ｍｅｔｈｏｄｓ：Ｔｈｅ ｔｏｘｉｃｉｔｙ ｏｆ ＤＯＰ ｗａｓ ｍｅａｓｕｒｅｄ ｗｉｔｈ ｌｅａｆ-ｄｉｐｐｉｎｇ ｍｅｔｈｏｄ． Ｔｈｅ ａｃｔｉｖｉｔｅｓ ｏｆ ｅｎｚｙｍｅｓ ｗｅｒｅ ｄｅｔｅｒｍｉｎｅｄ ｗｉｔｈ ＡＣｈＥ ａｎｄ ＡＴＰａｓｅ ａｓｓａｙ ｋｉｔ． Ｒｅｓｕｌｔ：① ＤＯＰ ｅｘｈｉｂｉｔｅｄ ｔｏｘｉｃｉｔｙ ａｇａｉｎｓｔ ３ｒｄ ｉｎｓｔａｒ ｌａｒｖａｅ ｏｆ Ｐ． ｒａｐａｅ Ｌ． Ｔｈｉｓ ｔｏｘｉｃｉｔｙ ｉｓ ｃｏｎｃｅｎｔｒａｔｉｏｎ-ｔｉｍｅ-ｄｅｐｅｎｄｅｎｔ ｗｉｔｈ ＬＣ５０＝１５１．８７ ｍｇ／Ｌ（ａｔ ４８ ｈ）＞ＬＣ５０＝３９．９９ ｍｇ／Ｌ （ａｔ ７２ ｈ）＞ ＬＣ５０＝１０．６０ ｍｇ／Ｌ（ａｔ ９６ ｈ）．② ＤＯＰ ｄｉｄ ｎｏｔ ａｆｆｅｃｔ ｔｈｅ ａｃｔｉｖｉｔｙ ｏｆ ＡＣｈＥ． ③ ＤＯＰ ｐｒｏｆｏｕｎｄｌｙ ｉｎｈｉｂｉｔｅｄ ｔｈｅ ａｃｔｉｖｉｔｙ ｏｆ ＡＴＰａｓｅ ａｔ ｈｉｇｈ ｃｏｎｃｅｎｔｒａｔｉｏｎ（１２５ ｍｇ／Ｌ）． ＤＯＰ ａｔ ｍｉｄｄｌｅ ｃｏｎｃｅｎｔｒａｔｉｏｎ（５，２５ ｍｇ／Ｌ） ｓｈｏｗｅｄ ａｃｔｉｖａｔｉｖｅ ｅｆｆｅｃｔ ａｔ ２４ ｈ，ｔｈｅｎ ｒｅｃｏｖｅｒｅｄ ａｔ ４８ ｈ， ａｎｄ ｍａｉｎｔａｉｎｅｄ ｎｏｒｍａｌ（５ ｍｇ／Ｌ） ｏｒ ｅｘｈｉｂｉｔｅｄ ｉｎｈｉｂｉｔｏｒｙ ｅｆｆｅｃｔ（２５ ｍｇ／Ｌ） ａｔ ７２ ｈ． Ｃｏｎｃｌｕｓｉｏｎ：ＤＯＰ ｅｘｈｉｂｉｔｅｄ ｔｏｘｉｃｉｔｙ ａｇａｉｎｓｔ ３ｒｄ ｉｎｓｔａｒ ｌａｒｖａｅ ｏｆ Ｐ． ｒａｐａｅ Ｌ． ＡＣｈＥ ｉｓ ｎｏｔ ｔａｒｇｅｔ ｏｆ ｄｅｏｘｙｐｏｄｏｐｈｙｌｌｏｔｏｘｉｎ ａｎｄ ＡＴＰａｓｅｓ ｍａｙ ｂｅ ｏｎｅ ｉｍｐｏｒｔａｎｔ ｔａｒｇｅｔ ｏｆ ｄｅｏｘｙｐｏｄｏｐｈｙｌｌｏｔｏｘｉｎ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｃｙｔｏｔｏｘｉｃｉｔｙ ｏｆ ｂｒｅａｓｔ ｃａｎｃｅｒ ｃｅｌｌ ｌｉｎｅ ＭＣＦ-７ ｃｅｌｌｓ ｉｎｄｕｃｅｄ ｂｙ ｔｒｉｃｈｏｓｔａｔｉｎ Ａ ａｎｄ ｉｔｓ ｐｏｓｓｉｂｌｅ ｔｒａｎｓｃｒｉｐｔｉｏｎ ｒｅｇｕｌａｔｉｏｎ． Ｍｅｔｈｏｄｓ：Ｔｈｅ ｐｒｏｌｉｆｅｒａｔｉｖｅ ａｃｔｉｖｉｔｙ ｏｆ ＭＣＦ-７ ｃｅｌｌｓ ｕｐｏｎ ｄｉｆｆｅｒｅｎｔ ｔｒｉｃｈｏｓｔａｔｉｎ Ａ ｃｏｎｃｅｎｔｒａｔｉｏｎ ｗａｓ ａｃｃｅｓｓｅｄ ｂｙ ＭＴＴ ａｓｓａｙ， ｃｅｌｌ ａｐｏｐｔｏｓｉｓ ｆｏｌｌｏｗｅｄ ｗａｓ ｉｄｅｎｔｉｆｉｅｄ ｂｙ Ａｎｎｅｘｉｎ-Ｖ／ＰＩ ｄｏｕｂｌｅ ｓｔａｉｎｉｎｇ； ｃｙｃｌｅ ａｌｔｅｒａｔｉｏｎ ｗａｓ ｅｘａｍｉｎｅｄ ｂｙ ｃｅｌｌ ｃｙｃｌｅ ａｎａｌｙｓｉｓ ｉｎ ｄｉｆｆｅｒｅｎｔ ｔｉｍｅ， ｅｘｐｒｅｓｓｉｏｎ ｓｔａｔｕｓ ｏｆ ＥＲα， ｍｙｃ-ｃ， Ｐ２１， ｃｙｃｌｉｎ-Ｄ ａｎｄ Ｂｃｌ-２ ｇｅｎｅｓ ｗａｓ ａｎａｌｙｚｅｄ ｂｙ ＲＴ-ＰＣＲ． Ｒｅｓｕｌｔｓ：Ａ ｔｉｍｅ ａｎｄ ｄｏｓｅ ｄｅｐｅｎｄｅｎｔ ｉｎｈｉｂｉｔｉｏｎ ｗａｓ ｄｅｔｅｃｔｅｄ ｉｎ ＭＣＦ-７ ｃｅｌｌｓ ｔｒｅａｔｅｄ ｗｉｔｈ ｔｒｉｃｈｏｓｔａｉｎ Ａ． Ａｔ ４８ ｈ ｔｒｅａｔｍｅｎｔ， Ａｎｎｅｘｉｎ-Ｖ／ＰＩ ｄｏｕｂｌｅ ｓｔａｉｎｉｎｇ ａｎａｌｙｓｉｓ ｉｎｄｉｃａｔｅｄ ｔｈａｔ ｃｅｌｌ ａｐｏｐｔｏｓｉｓ ｒａｔｅ ｉｎｃｒｅａｓｅｄ ａｓ ｔｒｉｃｈｏｓｔａｔｉｎ Ａ ｒｏｓｅ， ａｔ ０．５ -滋ｍｏｌ／Ｌ ｔｒｅａｔｍｅｎｔ， ａｐｏｐｔｏｓｉｓ ｒａｔｅ ｗａｓ ３３．８２％； Ｃｅｌｌ ｃｙｃｌｅ ａｎａｌｙｓｉｓ ｓｈｏｗｅｄ ＭＣＦ-７ ｃｅｌｌｓ ｗｅｒｅ ａｒｒｅｓｔｅｄ ｉｎ ｃｙｃｌｅｓ ａｆｔｅｒ ｔｒｉｃｈｏｓｔａｔｉｎ Ａ ｔｒｅａｔｍｅｎｔ， ｗｈｉｌｅ ａｌｍｏｓｔ ｎｏ ａｐｏｐｔｏｓｉｓ ｗａｓ ｅｖｉｄｅｎｃｅｄ． ＲＴ-ＰＣＲ ｒｅｖｅａｌｅｄ ｔｈａｔ ｅｘｃｅｐｔ Ｐ２１ ｇｅｎｅ，ＥＲα，ｍｙｃ-ｃ，ｃｙｃｌｉｎ-Ｄ ａｎｄ Ｂｃｌ-２ ｗｅｒｅ ａｌｌ ｄｏｗｎ ｒｅｇｕｌａｔｅｄ，ａｎｄ ａｌｌ ｍａｄｅ ｉｔ ｔｏ ｐｒｏｌｉｆｅｒａｔｉｖｅ ｉｎｈｉｂｉｔｉｏｎ， ｃｅｌｌ ａｒｒｅｓｔ ａｎｄ ａｐｏｐｔｏｓｉｓ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｒｉｃｈｏｓｔａｔｉｎ Ａ ｃｏｕｌｄ ｉｎｄｕｃｅ ｃｙｔｏｔｏｘｉｃｉｔｙ ｉｎ ＭＣＦ-７ ｃｅｌｌｓ， ａｎｄ ｍａｙ ｃｏｒｒｅｌａｔｅ ｗｉｔｈ ｔｒａｎｓｃｒｉｐｔｉｏｎ ｒｅｇｕｌａｔｉｏｎ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｃｌｏｎｅ ａｎｄ ａｎａｌｙｚｅ ｔｈｅ ｄｉｆｆｅｒｅｎｔ ｓｐｌｉｃｉｎｇ ｖａｒｉａｎｔ ｏｆ Ｌｉｍｓ ｇｅｎｅ ｉｎ ｍｏｕｓｅ． Ｍｅｔｈｏｄｓ：Ｓｐｌｉｃｉｎｇ ｖａｒｉａｎｔｓ ｏｆ Ｌｉｍｓ ｇｅｎｅ ｗｅｒｅ ａｍｐｌｉｆｉｅｄ ｂｙ ＲＴ-ＰＣＲ ｆｒｏｍ ｍｏｕｓｅ ｃＤＮＡ ａｎｄ ｉｎｓｅｒｔｅｄ ｉｎｔｏ ＰｉｎＰｏｉｎｔＴＭ Ｘａ-１Ｔ ｖｅｃｔｏｒ， ｔｗｏ ｐｏｓｉｔｉｏｎ ｃｌｏｎｅｓ ｓｅｌｅｃｔｅｄ ｂｙ ＰＣＲ ｗｅｒｅ ｓｅｑｕｅｎｃｅｄ． Ｒｅｓｕｌｔｓ：Ｌｉｓｍ Ｅ， ａ ｎｏｖｅｌ ｓｐｌｉｃｉｎｇ ｖａｒｉａｎｔ ｗｉｔｈ ａ １１６４ ｂｐ ｏｐｅｎ ｒｅａｄｉｎｇ ｆｒａｍｅ（ＯＲＦ）， ｅｎｃｏｄｉｎｇ ａ ３８７-ａｍｉｎｏ ａｃｉｄ（ＡＡ） ｐｒｏｔｅｉｎ ｗａｓ ｃｌｏｎｅｄ． Ｃｏｎｃｌｕｓｉｏｎ：Ｃｏｍｐａｒａｔｉｖｅ ｇｅｎｏｍｅ ａｎａｌｙｓｉｓ ｄｉｓｐｌａｙｅｄ Ｌｉｍｓ Ｅ， ａ ｎｏｖｅｌ ｖａｒｉａｎｔ ｏｆ Ｌｉｍｓ ｇｅｎｅ ｗａｓ ｃｏｎｆｉｒｍｅｄ ｉｎ ｍｏｕｓｅ ａｎｄ ｅｓｔａｂｌｉｓｈｅｄ ｔｈｅ ｆｏｕｎｄａｔｉｏｎ ｆｏｒ ｆｕｒｔｈｅｒ ｔｏ ｓｔｕｄｙｉｎｇ ｔｈｅ ｆｕｎｃｔｉｏｎ ｏｆ Ｌｉｍｓ Ｅ ｉｎ ｃｅｌｌ ｄｅｖｅｌｏｐｍｅｎｔ．

Abstract:Objective：To investigate the protein expression of GATA-4 in the developing rat hearts. Methods：Embryolc hearts of normal rats were obtained on the 12 th, 15 th and 19 th day during embryonic period. The expression level of GATA-4 protein was accessed by Western blot and Immunohistochemistry, and the results were analyzed by the statistical method of One-way ANOVA. Results：GATA-4 was expressed in atrial ventricular myocardium,endocardium, endocardial cushion and the root of main artery, Ｔhe expression of GATA-4 in the heart was ｉｎｃｒｅａｓｉｎｇ ｏｂｖｉｏｕｓｌｙ ｏｎ １５ ｔｈ ｄａｙ ｉｎ ｅｍｂｒｙｏｎｉｃ ｐｅｒｉｏｄ, but it was slightly ｄｅｃｒｅａｓｉｎｇ ｏｎ １９ ｔｈ ｄａｙ． Conclusion：With the development of rat hearts, the protein expression of GATA-4 was dynamic. It was suggested that GATA-4 should play a crucial role in rat heart development.

Abstract:Objective：To construct the TGF-β3 protein with targeted therapy function in which latency associated peptide, MMP enzyme and TGF-β3 play the role of latent, targeting and therapeutic effect, respectively. In addition, the specific targeted therapeutic effect was investigated by transferring TGF-β3 gene to chondrogenic progenitor cells（CPCs） from perichondrium. Methods：The recombinant of pIRES-EGFP-MMP was constructed by combination of DNA encoding MMP enzyme cutting site and eukaryotic expression vector pIRES-EGFP. LAP and TGF-β3 fragments were obtained from rat embryos by RT-PCR and inserted into the upstream and downstream of MMP from pIRES-EGFP-MMP, respectively, so as to construct the recombinant plasmid of pIRES-EGFP-LAP-MMP-mTGF-β3, which was then transferred to chondrogenic progenitor cells. The genetically modified CPCs were cultured with medium with or without MMP-1. The expression of Collagen II, Aggrecan and TIMP were detected at day 7,14 and 21. Results：pIRES-EGFP-LAP-MMP-mTGF-β3 was successfully constructed and identified by enzyme digestion and sequencing analysis. Only the medium with MMP enzyme can promote the chondrogenesis and the matrix production of genetically modified CPCs． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｅ ｅｎｇｉｎｅｅｒｉｎｇ ＴＧＦ-β３ ｃｏｕｌｄ ｈａｖｅ ｔａｒｇｅｔｅｄ ｔｈｅｒａｐｙ ｆｏｒ ｃａｒｔｉｌａｇｅ ｒｅｐａｉｒ ｉｎ ｆｕｔｕｒｅ．

Abstract:Objective：To explore the anatomical characteristics of the peripheral pulmonary arteries in lung by observing the cross-sectional images of Chinese digitized visible human and making three-dimensional reconstruction of the pulmonary artery system. Methods：A series CVH images of chest were consecutively observed, then the pulmonary arteries were labeled in these images. At last，the labeled images were inserted into computer to make three-dimensional reconstruction. Resultｓ：The pulmonary arteries could be recognized in CVH images and reconstructed. The three-dimensional images perfectly show the brunches of the pulmonary arteries, even the subsubsegmental arteries（fifth order） were able to be recognized. Conclusion：The research reconstructed and visualized the pulmonary arteries of the first Chinese Visible Human. The digitalized visible model of the pulmonary arteries can display the special conformation of this region using reconstructed three-dimensional images. It will help in anatomical teaching and provide morphological data for imaging diagnosis and operation of lung.

Abstract:Objective：To investigate the effect of prograf on remnant hepatocellular carcinoma cells in the liver receptors after liver transplantation for liver cancer, and to instruct the anti-rejection drug selection. Methods：MTT assay，RT-PCR and Western blot were used to examine the expression of c-met and TGF-α in the hepatocellular carcinoma cell line HepG2 cultured in vitro. Results：The expression of c-met and TGF-αin prograf group（at concentration of 5 μg／L） was much lower than that in the control group（P < 0.05）. Conclusion：Prograf can inhibit the proliferation of hepatocellular carcinoma cell.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｐｒｅｐａｒｅ ａｎｄ ｉｄｅｎｔｉｆｙ ｐｏｌｙ ａｓｐａｒｔｉｃ ａｃｉｄ-ｃｏ-ｇｌｕｔａｍｉｃ ａｃｉｄ-ｍｅｔｒｏｎｉｄａｚｏｌｅ（ＰＡＧ-ＭＴＩ） ｎａｎｏｐａｒｔｉｃｌｅｓ．Ｍｅｔｈｏｄｓ：Ｍｅｔｒｏｎｉｄａｚｏｌｅ ｗａｓ ｃｏｎｊｕｇａｔｅｄ ｉｎｔｏ ｐｏｌｙｍｅｒｓ． Ｔｈｅ ｓｔｒｕｃｔｕｒｅ， ｍｏｒｐｈｏｌｏｇｙ ａｎｄ ｐａｒｔｉｃｌｅ ｓｉｚｅ ｏｆ ＰＡＧ-ＭＴＩ ｗｅｒｅ ｃｈａｒａｃｔｅｒｉｚｅｄ ｂｙ ｉｎｆｒａｒｅｄ ｓｐｅｃｔｒｏｐｈｏｔｏｍｅｔｒｙ， ｕｌｔｒａｖｉｏｌｅｔ ｓｐｅｃｔｒｏｐｈｏｔｏｍｅｔｒｙ， ｔｒａｎｓｍｉｓｓｉｏｎ ｅｌｅｃｔｒｏｎ ｍｉｃｒｏｓｃｏｐｅ ａｎｄ ｌａｓｅｒ ｄｉｆｆｒａｃｔｉｏｎ-ｂａｓｅｄ ｐａｒｔｉｃｌｅ ｓｉｚｅ ａｎａｌｙｚｅｒ． Ｍｅｔｒｏｎｉｄａｚｏｌｅ ｒｅｌｅａｓｅ ｂｅｈａｖｉｏｒ ｆｒｏｍ ｔｈｅｓｅ ｐｏｌｙｍｅｒｓ ｗａｓ ａｌｓｏ ｄｅｔｅｒｍｉｎｅｄ ｉｎ ｖｉｔｒｏ． Ｒｅｓｕｌｔｓ：ＰＡＧ-ＭＴＩ ｎａｎｏｐａｒｔｉｃｌｅｓ ｗｅｒｅ ｓｐｈｅｒｉｃａｌ ｉｎ ｍｏｒｐｈｏｌｏｇｙ； ｄｒｕｇ ｌｏａｄｉｎｇｓ ｗｅｒｅ １２％， ａｎｄ ａｖｅｒａｇｅ ｓｉｚｅ ｏｆ ｎａｎｏｐａｒｔｉｃｌｅｓ ｗａｓ１９８．９ ｎｍ． Ｔｈｅ ｒｅｌｅａｓｅ ｏｆ ｍｅｔｒｏｎｉｄａｚｏｌｅ ｉｎ ＰＡＧ-ＭＴＩ ｗａｓ ｓｕｓｔａｉｎｅｄ ｏｂｖｉｏｕｓｌｙ． Ｔｈｅ ａｃｃｕｍｕｌａｔｉｏｎ ｒａｔｅ ｏｆ ｍｅｔｒｏｎｉｄａｚｏｌｅ ｗａｓ １２．１９％ ａｔ １ ｈ， ａｎｄ ４７．５１％ ａｔ ２４ ｈ ａｆｔｅｒ ｒｅｌｅａｓｅｄ． Ｃｏｎｃｌｕｓｉｏｎ：ＰＡＧ-ＭＴＩ ｎａｎｏｐａｒｔｉｃｌｅｓ ｂｙ ｃｈｅｍｏｓｙｎｔｈｅｓｉｓ ｃａｎ ｄｅｌａｙ ｍｅｔｒｏｎｉｄａｚｏｌｅ ｒｅｌｅａｓｅ ｉｎ ｖｉｔｒｏ． Ｉｔ ｍａｙ ｂｅ ｃｏｎｓｉｄｅｒｅｄ ａｓ ａ ｐｒｏｍｉｓｉｎｇ ａｐｐｒｏａｃｈ ｉｎ ｔｈｅ ｔｒｅａｔｍｅｎｔ ｏｆ Ｔ．ｖａｇｉｎａｌｉｓ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｓｔｕｄｙ ｔｈｅ ｃｏｒｒｅｌａｔｉｏｎ ｂｅｔｗｅｅｎ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｉｎ ｇａｓｔｒｉｃ ｃａｎｃｅｒ ｔｉｓｓｕｅｓ ａｎｄ ｔｈｅ ｔｕｍｏｒ ｌｙｍｐｈ ｎｏｄｅ ｍｅｔａｓｔａｓｉｓ ａｎｄ ｓｕｒｖｉｖａｌ ｒａｔｅ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ，ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｃａｎ ｂｅ ｕｓｅｄ ｔｏ ｅｓｔｉｍａｔｅ ｐｒｏｇｒｅｓｓｉｏｎ ａｎｄ ｐｒｏｇｎｏｓｉｓ． Ｍｅｔｈｏｄｓ：Ｕｓｉｎｇ ｉｎ ｓｉｔｕ ｈｙｂｒｉｄｉｚａｔｉｏｎ ｍｅｔｈｏｄ，６６ ｓｐｅｃｉｍｅｎｓ ａｎｄ １０ ｎｏｒｍａｌ ｔｉｓｓｕｅ ｓｐｅｃｉｍｅｎｓ（ｃｏｎｔｒｏｌｓ） ｗｅｒｅ ｔｅｓｔｅｄ ｆｏｒ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ． Ｔｈｅ ｓｕｒｖｉｖａｌ ｒａｔｅ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ ｗａｓ ｉｎｖｅｓｔｉｇａｔｅｄ ａｌｓｏ． Ｔｈｅ ｃｏｒｒｅｌａｔｉｏｎ ｂｅｔｗｅｅｎ ＴＧＦ-β１ｍＲＮＡ ａｎｄ ｓｕｒｖｉｖａｌ ｒａｔｅ ｗａｓ ａｎａｌｙｚｅｄ． Ｒｅｓｕｌｔｓ：Ｔｈｅ ｇａｓｔｒｉｃ ｃａｎｃｅｒ ｉｎ ６６ ｓｐｅｃｉｍｅｎｓ ｄｉｅｄ ｉｎ ５ ｙｅａｒ ｗａｓ ８８．４６％（２３／２６）． Ｔｈｅ ｐｏｓｉｔｉｖｅ ｅｘｐｒｅｓｓｉｏｎ ｒａｔｅ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｉｎ ｇａｓｔｒｉｃ ｃａｎｃｅｒ ｗａｓ ８６．３６％（５７／６６），ｗｈｉｌｅ ｉｔ ｗａｓ １０．００％（１／１０） ｉｎ ｃｏｎｔｒｏｌｓ，ｉｎｄｉｃａｔｉｎｇ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｔｈｅｍ（Ｐ ＜ ０．０１）． Ｔｈｅ ｎｅｇａｔｉｖｅ ａｎｄ ｌｏｗｅｒ ｐｏｓｉｔｉｖｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｉｎ ｔｈｏｓｅ ｗｈｏ ｄｉｅｄ ａｆｔｅｒ ５ ｙｅａｒｓ ｗａｓ ４７．５０％（１９／４０），ｉｎ ｔｈｏｓｅ ｗｈｏ ｄｉｅｄ ｉｎ ５ ｙｅａｒ ｗａｓ ５２．５０％（２１／４０），ｉｎｄｉｃａｔｉｎｇ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｔｈｅｍ（Ｐ ＜ ０．０５）． Ｔｈｅ ｍｉｄｄｌｅ ａｎｄ ｓｔｒｏｎｇ ｐｏｓｉｔｉｖｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｉｎ ｔｈｏｓｅ ｗｈｏ ｄｉｅｄ ａｆｔｅｒ ５ ｙｅａｒｓ ｗａｓ １１．５４％（３／２６）， ｉｎ ｔｈｏｓｅ ｗｈｏ ｄｉｅｄ ｉｎ ５ ｙｅａｒ ｗａｓ ８８．４６％（２３／２６）． Ｔｈｅ ｐｏｓｉｔｉｖｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｉｎ ｔｈｏｓｅ ｗｈｏ ｈａｖｅ ｎｏ ｔｕｍｏｒ ｌｙｍｐｈ ｎｏｄｅ ｍｅｔａｓｔａｓｉｓ ｗａｓ ７５．００％（２４／３２），ｗｈｉｌｅ ｉｔ ｗａｓ ９７．０６％（３３／３４） ｉｎ ｔｈｏｓｅ ｗｈｏ ｈａｖｅ ｔｕｍｏｒ ｌｙｍｐｈ ｎｏｄｅ ｍｅｔａｓｔａｓｉｓ，ｉｎｄｉｃａｔｉｎｇ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｔｈｅｍ（Ｐ ＜ ０．０５）． Ｔｈｅｒｅ ｗａｓ ｐｏｓｉｔｉｖｅ ｃｏｒｒｅｌａｔｉｏｎ ｂｅｔｗｅｅｎ ｔｈｅ ｈｉｇｈｅｒ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ａｎｄ ｔｈｅ ｗｏｒｓｅ ｐｒｏｇｎｏｓｉｓ ａｎｄ ｒａｐｉｄ ｐｒｏｇｒｅｓｓｉｏｎ ｏｆ ｇａｓｔｒｉｃ ｃａｎｃｅｒ． Ｃｏｎｃｌｕｓｉｏｎ：ＴＧＦ-β１ ｃａｎ ｓｔｉｍｕｌａｔｅ ｇａｓｔｒｉｃ ｃａｎｃｅｒ ｇｒｏｗ ａｎｄ ｂｅｃｏｍｅ ｍｏｒｅ ａｇｇｒｅｓｓｉｖｅ ａｎｄ ｔｈｅ ｔｕｍｏｒ ｌｙｍｐｈ ｍｅｔａｓｔａｓｉｓ． Ｔｈｅ ｄｅｔｅｃｔｉｏｎ ｏｆ ＴＧＦ-β１ｍＲＮＡ ｃａｎ ｂｅ ｕｓｅｄ ｔｏ ｅｓｔｉｍａｔｅ ｇａｓｔｒｉｃ ｃａｎｃｅｒ ｐａｔｉｅｎｔｓ，ｐｒｏｇｒｅｓｓｉｏｎ ａｎｄ ｐｒｏｇｎｏｓｉｓ．

Abstract:Objective：To assess the hemocompatibility of biomaterial covalently coated by chitosan／heparin designed for the closure of congenital heart defects in vitro. Methods：Chitosan（Chi）／heparin（Hep） was covalently immobilized onto the surface of Nitinol slices. The hemocompatibility of biomaterials was evaluated by using the hemolysis test． Ａnticoagulant activity was studied in vitro in a dynamic model with whole blood, and partial thrombin time（APTT）, prothrombin time（PT）, fibrinogen time, thrombin time（TT） and D-Dimer respectively. Surface characterizations such as protein adsorption and platelet adhesion were performed by using scanning electron microscopy. Results：White blood count, red blood count and platelet count（PC） were all in normal range and there were no evident difference in each group. The hemolysis rates of biomaterials was all less than 5％. Chi／Hep did significantly prolong the APTT, PT, and TT． In addition, the APTT, PT, and TT in groups coated with different concentration of Hep showed no significant difference. The immobilization of Chi／Hep caused the protein adsorption, platelet adhesion, and thrombus formation were all reduced by the immobilization of Hep. Conclusion：In vitro hemocompatibility of biomaterials designed for the closure of congenital heart defects can be improved by conjugating Chi／Hep onto the surface.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ａｓｓｅｓｓ ｔｈｅ ｓａｆｅｔｙ ａｎｄ ｅｆｆｉｃａｃｙ ｏｆ ｔｈｅ ｔｒａｎｓｃａｔｈｅｔｅｒ ｃｌｏｓｕｒｅ ｏｆ ｐａｔｅｎｔ ｄｕｃｔｕｓ ａｒｔｅｒｉｏｓｕｓ（ＰＤＡ）． Ｍｅｔｈｏｄｓ：Ａ ｆｏｌｌｏｗ-ｕｐ ｓｔｕｄｙ ｗａｓ ｃｏｎｄｕｃｔｅｄ ｉｎ ｐａｔｉｅｎｔｓ ｗｉｔｈ ＰＤＡ ｕｎｄｅｒｗｅｎｔ ｔｒａｎｓｃａｔｈｅｔｅｒ ｃｌｏｓｕｒｅ ｉｎ ｏｕｒ ｈｏｓｐｉｔａｌ． Ａｌｌ ｐａｔｉｅｎｔｓ ｗｅｒｅ ａｓｓｅｓｓｄ ｗｉｔｈ ｐｈｙｓｉｃａｌ ｅｘａｍｉｎａｔｉｏｎ， Ｘ-ｒａｙ， ａｎｄ ｅｃｈｏｃａｒｄｉｏｇｒａｐｈｙ ｂｅｆｏｒｅ ｔｈｅ ｏｐｅｒａｔｉｏｎ． Ｄｕｒｉｎｇ ｔｈｅ ｆｏｌｌｏｗ-ｕｐ ｔｉｍｅ， ｔｈｅ ｐａｔｉｅｎｔｓ’ ｃａｒｄｉａｃ ｆｕｎｃｔｉｏｎ ａｎｄ ｃｏｍｐｌｉｃａｔｉｏｎｓ ｗｅｒｅ ｅｖａｌｕａｔｅｄ． Ｒｅｓｕｌｔｓ：１７５ ｐａｔｉｅｎｔｓ ｕｎｄｅｒｗｅｎｔ ｔｒａｎｓｃａｔｈｅｔｅｒ ｃｌｏｓｕｒｅ ｈａｄ ｂｅｅｎ ｆｏｌｌｏｗｅｄ ｕｐ． Ｔｈｅ ｐａｔｉｅｎｔｓ ｗｅｒｅ ｆｏｌｌｏｗｅｄ ｕｐ ｆｒｏｍ １ ｔｏ ７７ ｍｏｎｔｈｓ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ． Ｍｉｎｉｍａｌ ｒｅｓｉｄｕｅ ｓｈｕｎｔｓ ｗｅｒｅ ｆｏｕｎｄ ｉｎ ９ ｃａｓｅｓ（５．１４％） ｉｍｍｅｄｉａｔｅｌｙ ａｆｔｅｒ ｃｌｏｓｕｒｅ． ３ ｃａｓｅｓ（１．７１％） ｗｅｒｅ ｄｅｔｅｃｔｅｄ ｉｎｃｒｅａｓｅｄ ｂｌｏｏｄ ｖｅｌｏｃｉｔｙ ｉｎ ｄｅｓｃｅｎｄｉｎｇ ａｏｒｔａ ｏｒ ｐｕｌｍｏｎａｒｙ ａｏｒｔａ． Ａｔ １ ｍｏｎｔｈ ｆｏｌｌｏｗ-ｕｐ，ｎｏ ｒｅｓｉｄｕｅ ｓｈｕｎｔ ｗａｓ ｆｏｕｎｄ． ３ ｃａｓｅｓ ｗｉｔｈ ｉｎｃｒｅａｓｅｄ ｂｌｏｏｄ ｖｅｌｏｃｉｔｙ ｒｅｔｕｒｎｅｄ ｔｏ ｎｏｒｍａｌ ｄｕｒｉｎｇ １～５ ｙｅａｒｓ ｆｏｌｌｏｗ-ｕｐ． Ｔｈｅ ｈｅａｒｔ ｓｔｒｕｃｔｕｒｅ ｗａｓ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｃｈａｎｇｅｄ １ ｗｅｅｋ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ． Ｔｈｅ ｍｅａｎ ｖａｌｕｅ ｏｆ ｌｅｆｔ ａｔｒｉａ ｄｉａｍｅｔｅｒ， ｌｅｆｔ ｖｅｎｔｒｉｃｕｌａｒ ｅｎｄ ｓｙｓｔｏｌｉｃ ｄｉａｍｅｔｅｒ，ｌｅｆｔ ｖｅｎｔｒｉｃｕｌａｒ ｅｎｄ ｄｉａｓｔｏｌｉｃ ｄｉａｍｅｔｅｒ，ｌｅｆｔ ｖｅｎｔｒｉｃｕｌａｒ ｅｎｄ ｓｙｓｔｏｌｉｃ ｖｏｌｕｍｅ ａｎｄ ｌｅｆｔ ｖｅｎｔｒｉｃｕｌａｒ ｅｎｄ ｄｉａｓｔｏｌｉｃ ｖｏｌｕｍｅ ｍｅａｓｕｒｅｄ ｂｙ ｅｃｈｏｃａｒｄｉｏｇｒａｐｈｙ ｂｅｆｏｒｅ ｔｈｅ ｏｐｅｒａｔｉｏｎ ｗｅｒｅ（３１．６±７．８）ｍｍ，（３３．０±７．６）ｍｍ，（５１．６±１０．６）ｍｍ，（４０．３±１９．５）ｍｌ，（１１８．６±３２．５）ｍｌ ａｎｄ（７７．７±３８．１）ｍｌ，ｒｅｓｐｅｃｔｉｖｅｌｙ． Ｔｈｅｓｅ ｖａｌｕｅｓ ｗｅｒｅ ｄｅｃｒｅａｓｅｄ ｔｏ（２９．４±６．６）ｍｍ，（３０．８±６．７）ｍｍ，（４７．５±９．２）ｍｍ，（３４．６±１９．０）ｍｌ， （８０．３±２９．６）ｍｌ ａｎｄ（６４．５±３１．１）ｍｌ， ｒｅｓｐｅｃｔｉｖｅｌｙ， １ ｗｅｅｋ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ（Ｐ ＜ ０．０５）． Ｔｈｅｒｅ ｗａｓ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｂｅｔｗｅｅｎ ｐｒｅ-ｏｐｅｒａｔｉｏｎ ａｎｄ １-ｗｅｅｋ ｏｒ １-ｍｏｎｔｈ ａｆｔｅｒ ｏｐｅｒａｔｉｏｎ． Ｔｈｅｒｅ ｗｅｒｅ ｎｏ ｓｅｖｅｒｅ ｃｏｍｐｌｉｃａｔｉｏｎｓ ｏｒ ｄｅａｔｈｓ ｉｎ ｔｈｉｓ ｇｒｏｕｐ ｏｆ ｐａｔｉｅｎｔｓ ｄｕｒｉｎｇ ｆｏｌｌｏｗ-ｕｐ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｉｓ ｓｔｕｄｙ ｓｕｇｇｅｓｔｅｄ ｔｒａｎｓｃａｔｈｅｔｅｒ ｃｌｏｓｕｒｅ ｏｆ ＰＤＡ ｃｏｕｌｄ ｉｍｐｒｏｖｅ ｃａｒｄｉａｃ ｆｕｎｃｔｉｏｎ ｏｆ ｔｈｅｓｅ ｐａｔｉｅｎｔｓ， ａｎｄ ａｌｓｏ ｃｏｎｆｉｒｍｅｄ ｔｈｅ ｓａｆｅｔｙ ａｎｄ ｅｆｆｉｃａｃｙ ｏｆ ｔｈｏｓｅ ｄｕｒｉｎｇ ｓｈｏｒｔ-ｔｅｒｍ ａｎｄ ｌｏｎｇ-ｔｅｒｍ ｆｏｌｌｏｗ-ｕｐｓ．

Abstract:Objective：To evaluate ventricular segments motion on AAI mode and DDD mode with optimal atrioventricular interval by the quantitative tissue velocity imaging（QTVI）， and to investigate whether AAI pacing mode was superior ｔｏ the DDD pacing mode with optimal atrioventricular interval in sick sinus syndrome（SSS） patients withⅠdegree atrioventricular block（I°AVB）. Methods：Twenty four patients with implanted DDD pacemakers for SSS with I°AVB（PR interval ＞ 2０0 ms and < 260 ms） were studied at four-chamber view, two-chamber view and apical longitudinal view using QTVI. The regional parameters, including peak sustained velocity（Vs）, peak early diastolic velocity（Ve）, peak late diastolic velocity（Va） and peak systolic displacement（D） of the basal and middle segments in left ventricle（anterior septal, anterior, lateral, posterior, inferior and septal wall） and in the free wall of right ventricle were measured. Results：There were significant differences in the parameters（Vs, Ve） of most basal segments between AAI group and DDD group（P ＜ 0.05）. There were no significant differences in other parameters between both modes（P ＞ 0.05）. Conclusion：① In AAI pacing mode, ventricular systolic and diastolic function are superior to DDD pacing mode with optimal atrioventricular interval in patients with SSS, which intrinsic AV conduction are moderately prolonged; ②The change of ventricle segments motion can be early evaluated by QTVI in AAI and DDD mode, and it is useful to determine the optimal pacing mode in SSS patients withⅠ° AVB.

Abstract:Objective：To evaluation the role of myocardial perfusion ，viability and MRI cine in myocardial infarction of pig model and patients. Methods：14 pigs with myocardial infarction（3 days），12 pigs with myocardial infarction（3 weeeks） and 17 patients underwent MR scanning. MR cine imaging acquaired by BFFE sequence were used to analyze the motion of heart. T1TFE sequence was used to detect the first pass perfusion after injection of Gd-DTPA（0.1 mmol／kg）. Inversion recovery viability sequence was used to recognize the high signal in the infarct myocardia. Results：The incidence of the low signal detected in the first pass perfusion weighted images in the infarct myocardia of 3-days ｐigs，3-weeks pigs and 17 patients were respectively 100％，92％and 82.3％. The incidence of enhancement region detected in the delayed viability sequence images in the infarct myocardia of 3-days pigs，3-weeks pigs and 17 patients were respectively 7％, 50％and 88.2％. The myocardial wall of hyper enhancement had a weaken motion in MR cine images. The symptom of attenuation and keeping low signal of myocardial wall in viability images was detected in the obvious fibrosis myocardia or scar. Conclusion：The combination of all image findings in the first pass perfusion, viability imaging and cine MR might discriminate the true infarction and scar of heart effectirely.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｅｘｐｌｏｒｅ ｔｈｅ ｓｉｇｎｉｆｉｃａｎｃｅ ｌｏｓｓ ｏｆ Ｋａｉ-１ ｇｅｎｅ ｐｌａｙｓ ｉｎ ｔｈｅ ｐｒｏｇｒｅｓｓｉｏｎ ａｎｄ ｍｅｔａｓｔａｓｉｓ ｏｆ ｃｏｌｏｒｅｃｔａｌ ｃａｎｃｅｒ （ＣＲＣ）． Ｍｅｔｈｏｄｓ：Ｔｈｅ ｄｅｌｅｔｉｏｎ ｏｆ Ｋａｉ-１ ｇｅｎｅ ｗｅｒｅ ｄｅｔｅｃｔｅｄ ｂｙ ｕｓｉｎｇ ＲＴ-ＰＣＲ ａｎｄ ｖａｌｉｄａｔｅｄ ｂｙ ｓｅｑｕｅｎｃｉｎｇ ｉｎ ｔｈｅ ｔｕｍｏｒ ｏｆ ４０ ｃａｓｅｓ ｏｆ ＣＲＣ ａｎｄ １５ ｎｏｒｍａｌ ｃａｓｅｓ ｎｅａｒ ｔｈｅ ｔｉｓｓｕｅｓ ｏｆ ＣＲＣ． Ｒｅｓｕｌｔｓ：Ｔｈｅ ｄｅｌｅｔｉｏｎ ｏｆ ｅｘｏｎ ９ ｉｎ ｔｈｅ Ｋａｉ-１ ｇｅｎｅ ｗａｓ ｆｏｕｎｄ ｉｎ １８ ｏｆ ４０ ｃｏｌｏｒｅｃｔａｌ ｃａｓｅｓ（４５％） ａｎｄ ｉｎ ２ ｏｆ １５ ｎｏｒｍａｌ ｃａｓｅｓ ｎｅａｒ ｔｈｅ ｃｏｌｏｒｅｃｔａｌ ｔｉｓｓｕｅｓ（１３．３％）． Ｔｈｅ ｆｒｅｑｕｅｎｃｙ ｏｆ ｔｈｅ ｅｘｏｎ ９ ｄｅｌｅｔｉｏｎ ｗａｓ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｈｉｇｈｅｒ ｉｎ ｔｈｅ ｃｏｌｏｒｅｃｔａｌ ｔｕｍｏｒｓ ｔｈａｎ ｉｎ ｔｈｅ ｎｏｒｍａｌ ｃａｓｅｓ（Ｐ ＜ ０．０５）． Ｔｈｅ ｆｒｅｑｕｅｎｃｙ ｏｆ ｔｈｅ ｅｘｏｎ ９ ｄｅｌｅｔｉｏｎ ｗａｓ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｈｉｇｈｅｒ ｉｎ ｔｈｅ ｃｏｌｏｒｅｃｔａｌ ｔｕｍｏｒｓ ｗｉｔｈ ｌｙｍｐｈａｔｉｃ ｍｅｔａｓｔａｓｉｓ ｔｈａｎ ｔｈａｔ ｗｉｔｈｏｕｔ ｌｙｍｐｈａｔｉｃ ｍｅｔａｓｔａｓｉｓ（Ｐ ＜ ０．０５）， ａｎｄ ｉｎ ｔｈｅ ａｄｖａｎｃｅｄ ｓｔａｇｅ（Ｄｕｋｅｓ Ｃ，Ｄ） ｔｈａｎ ｉｎ ｔｈｅ ｅａｒｌｉｅｒ ｓｔａｇｅ（Ｄｕｋｅｓ Ａ，Ｂ） ｏｆ ｔｈｅ ｃｏｌｏｒｅｃｔａｌ ｃａｎｃｅｒ（Ｐ ＜ ０．０５）． Ｈｏｗｅｖｅｒ， ｔｈｅｒｅ ｗａｓ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｄｉｆｆｅｒｅｎｃｅ ｏｆ ｔｈｅ ｆｒｅｑｕｅｎｃｙ ｏｆ ｅｘｏｎ ９ ｄｅｌｅｔｉｏｎ ｗｈｅｎ ｓｔｒａｔｅｆｉｅｄ ｂｙ ｔｈｅ ｔｈｅ ａｇｅ， ｓｅｘ， ｈｉｓｔｏｌｏｇｉｃａｌ ｔｙｐｅ ａｓ ｗｅｌｌ ａｓ ｄｉｆｆｅｒｅｎｔｉａｔｅｄ ｄｅｇｒｅｅ ｏｆ ｔｈｅ ｐａｔｉｅｎｔｓ ｗｉｔｈ ＣＲＣ（Ｐ ＞ ０．０５）． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｅ ｄｅｌｅｔｉｏｎ ｏｆ ｔｈｅ Ｋａｉ-１ ｗａｓ ａｓｓｏｃｉａｔｅｄ ｗｉｔｈ ｔｈｅ ｐｒｏｇｒｅｓｓｉｏｎ ａｎｄ ｍｅｔａｓｔａｓｉｓ ｏｆ ＣＲＣ， ａｎｄ ｔｈｅ ｄｅｔｅｃｔｉｏｎ ｏｆ ｔｈｅ ｄｅｌｅｔｉｏｎ ｏｆ ｔｈｅ Ｋａｉ-１ ｃａｎ ｂｅ ｃｏｎｓｉｄｅｒｅｄ ａｓ ａｎ ｏｂｊｅｃｔｉｖｅ ｃｌｉｎｉｃａｌ ｍａｒｋｅｒ ｔｏ ａｓｓｅｓｓ ｔｈｅ ｐｒｏｇｒｅｓｓｉｏｎ ａｎｄ ｍｅｔａｓｔａｓｉｓ ｏｆ ＣＲＣ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｓｔｕｄｙ ｔｈｅ ｉｎｆｌｕｅｎｃｅｓ ｏｎ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ｖａｓｃｕｌａｒ ｅｎｄｏｔｈｅｌｉｕｍ ｇｒｏｗｔｈ ｆａｃｔｏｒ（ＶＥＧＦ） ａｎｄ ｎｅｒｖｏｕｓ ｃｏｎｄｕｃｔｉｏｎ ｂｙ ｉｍｐｒｏｖｉｎｇ ｍｉｃｒｏｃｉｒｃｕｌａｔｉｏｎ ｆｏｌｌｏｗｉｎｇ ｃｒｕｓｈｉｎｇ ｌｅｓｉｏｎ ｏｆ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｉｎ ｒａｔｓ． Ｍｅｔｈｏｄｓ：Ｓｃｉａｔｉｃ ｎｅｒｖｅ ｗａｓ ｃｒｕｓｈｅｄ ｕｓｅｄ ｐｉｎｃｅｒｓ ｔｏ ｅｓｔａｂｌｉｓｈ ｔｈｅ ｍｏｄｅｌ ｏｆ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｃｒｕｓｈ ｌｅｓｉｏｎ ｉｎ ｒａｔｓ． Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＶＥＧＦ ｉｎ ｄｏｒｓａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ａｔ １２ ｈ，７２ ｈ，７ ｔｈ ｄａｙｓ ａｎｄ ｔｈｅ ｄｉｓｔａｎｃｅ ｂｅｔｗｅｅｎ ｒｉｇｈｔ ｂｅｈｉｎｄ ｔｏｅｓ， ｎｅｒｖｅ ｃｏｎｄｕｃｔｉｏｎ ｖｅｌｏｃｉｔｙ ｏｆ ｓｃｉａｔｉｃ ｎｅｒｖｅ ａｔ ４ｔｈ ｗｅｅｋ ｗｅｒｅ ｅｘａｍｉｎｅｄ ｒｅｓｐｅｃｔｉｖｅｌｙ ｂｙ ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｃａｌ ａｎｄ ｅｌｅｃｔｒｏｐｈｙｓｉｏｌｏｇｉｃ ｔｅｃｈｎｉｑｕｅｓ． Ｔｈｅｎ ｔｈｅｉｒ ｒｅｓｕｌｔｓ ｗｅｒｅ ｃｏｍｐａｒｅｄ ｗｉｔｈ ｅａｃｈ ｏｔｈｅｒ． ＳＤ ｒａｔｓ ｗｅｒｅ ｄｉｖｉｄｅｄ ｉｎｔｏ ｃｏｎｔｒｏｌ ｇｒｏｕｐ， ｍｏｄｅｌ ｇｒｏｕｐ， ａｌｐｒｏｓｔａｄｉｌ（Ｌｉｐｏ-ＰＧＥ１） ｇｒｏｕｐ ａｎｄ ｂｕｆｌｏｍｅｄｉｌ ｈｙｄｒｏｃｈｌｏｒｉｄｅ ｇｒｏｕｐ ａｔ ｒａｎｄｏｍ． Ｔｈｅｒｅ ｗｅｒｅ １６ ｒａｔｓ ｉｎ ｅａｃｈ ｇｒｏｕｐ． ＳＰＳＳ １０．０ ｗａｓ ｕｓｅｄ ｓｔａｔｉｓｔｉｃａｌｌｙ． Ｒｅｓｕｌｔｓ：①Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＶＥＧＦ ｉｎ ｄｏｒｓａｌ ｇａｎｇｌｉｏｎ ｃｅｌｌｓ ｉｎｃｒｅａｓｅｄ ｆｒｏｍ １２ ｈ ａｆｔｅｒ ｃｒｕｓｈ ｉｎｊｕｒｙ ｏｆ ｔｈｅ ｎｅｒｖｅ， ａｎｄ ｈａｖｅ ｉｎｃｒｅａｓｅｄ ｔｏ ｔｈｅ ｈｉｇｈｅｓｔ ａｔ ７２ ｈ ａｆｔｅｒ ｃｒｕｓｈ ｉｎｊｕｒｙ ｏｆ ｔｈｅ ｎｅｒｖｅ（Ｐ ＜ ０．０１）． Ｔｈｅｒｅ ｗａｓ ｎｏ ｉｎｃｒｅａｓｉｎｇ ｏｆ ｉｔ ｉｎ ｎｏｒｍａｌ ｇｒｏｕｐ． Ｉｔ ｉｎｃｒｅａｓｅｄ ｉｎ ａｌｐｒｏｓｔａｄｉｌ ｇｒｏｕｐ ａｎｄ ｂｕｆｌｏｍｅｄｉｌ ｈｙｄｒｏｃｈｌｏｒｉｄｅ ｇｒｏｕｐ ｍｏｒｅ ｔｈａｎ ｍｏｄｅｌ ｇｒｏｕｐ ｓｉｇｎｉｆｉｃａｎｔｌｙ（Ｐ ＜ ０．０１）． Ｉｔ ｂｅｃａｍｅ ｎｏｒｍａｌ ｏｎ １ ｔｈ ｗｅｅｋ ｉｎ ａｌｌ ｇｒｏｕｐｓ ａｆｔｅｒ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｃｒｕｓｈ ｉｎｊｕｒｙ． ②Ｏｎ ４ｔｈ ｗｅｅｋ ａｆｔｅｒ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｃｒｕｓｈ ｉｎｊｕｒｙ， ｄｉｓｔａｎｃｅ ｂｅｔｗｅｅｎ ｒｉｇｈｔ ｂｅｈｉｎｄ ｔｏｅｓ ａｎｄ ｎｅｒｖｏｕｓ ｃｏｎｄｕｃｔｉｏｎ ｖｅｌｏｃｉｔｙ ｏｆ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｉｎ ａｌｐｒｏｓｔａｄｉｌ ｇｒｏｕｐ ａｎｄ ｂｕｆｌｏｍｅｄｉｌ ｈｙｄｒｏｃｈｌｏｒｉｄｅ ｇｒｏｕｐ ｗｅｒｅ ｍｏｒｅ ｏｐｔｉｍａｌ ｔｈａｎ ｔｈｏｓｅ ｉｎ ｍｏｄｅｌ ｇｒｏｕｐ（Ｐ ＜ ０．０１） ａｎｄ ｎｏ ｍｏｒｅ ｏｐｔｉｍａｌ ｔｈａｎ ｔｈｏｓｅ ｉｎ ｎｏｒｍａｌ ｇｒｏｕｐ（Ｐ ＜ ０．０５）， ｗｈｉｌｅ ｔｈｅｒｅ ｗａｓ ｎｏｒｍａｌ ｉｎ ｃｏｎｔｒｏｌ ｇｒｏｕｐ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＶＥＧＦ ｉｎｃｒｅａｓｅ ａｎｄ ｔｈｅ ｎｅｒｖｏｕｓ ｃｏｎｄｕｃｔｉｏｎ ｖｅｌｏｃｉｔｙ ｏｆ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｅｌｅｖａｔｅ ｂｙ ｉｍｐｒｏｖｉｎｇ ｍｉｃｒｏｃｉｒｃｕｌａｔｉｏｎ ｓｉｇｎｉｆｉｃａｎｔｌｙ ａｆｔｅｒ ｓｃｉａｔｉｃ ｎｅｒｖｅ ｃｒｕｓｈ ｌｅｓｉｏｎ， ｓｏ ｔｈａｔ ｉｔ ｈａｓ ｉｍｐｒｏｖｉｎｇ ａｃｔｉｏｎ ｔｏ ｒｅｐａｉｒ ｐｅｒｉｐｈｅｒａｌ ｎｅｒｖｅ ｃｒｕｓｈ ｌｅｓｉｏｎ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｏｂｓｅｒｖｅ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｄｉｆｆｅｒｅｎｔ ｃｏｎｃｅｎｔｒａｔｉｏｎｓ ｏｆ ｔｈｉｏｐｅｎｔａｌ ａｎｄ Ｍｉｄａｚｏｌａｍ ｏｎ ｔｈｅ ｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ ｏｆ Ｔ ｈｅｌｐｅｒ ｃｅｌｌｓ ｉｎ ｖｉｔｒｏ ｉｎ ａｄｕｌｔｓ． Ｍｅｔｈｏｄｓ：Ｐｅｒｉｐｈｅｒａｌ ｖｅｎｏｕｓ ｂｌｏｏｄ ｓａｍｐｌｅｓ ｗｅｒｅ ｃｏｌｌｅｃｔｅｄ ｆｒｏｍ ２０ ｍｉｄｄｌｅ-ａｇｅｄ ｈｅａｌｔｈｙ ｄｏｎｏｒｓ． Ｉｎ ｅａｃｈ ｖｏｌｕｎｔｅｅｒ，２０ ｍｌ ｏｆ ｂｌｏｏｄ ｗａｓ ｄｉｖｉｄｅｄ ｉｎｔｏ ７ ｇｒｏｕｐｓ：ｃｏｎｔｒｏｌ ｇｒｏｕｐ，３ ｔｈｉｏｐｅｎｔａｌ ｇｒｏｕｐｓ（４ μｇ／ｍｌ，２０ μｇ／ｍｌ， ４０ μｇ／ｍｌ），３ ｍｉｄａｚｏｌａｍ ｇｒｏｕｐｓ（５０ ｎｇ／ｍｌ， １５０ ｎｇ／ｍｌ， ５００ ｎｇ／ｍｌ）． Ｗｈｏｌｅ ｂｌｏｏｄ ａｎｄ ｍｏｎｏｎｕｃｌｅａｒ ｃｅｌｌｓ ｆｒｏｍ ｐｅｒｉｐｈｅｒａｌ ｂｌｏｏｄ（ＰＢＭＣｓ） ｗｅｒｅ ｉｎｃｕｂａｔｅｄ ｗｉｔｈ ｄｉｆｆｅｒｅｎｔ ｃｏｎｃｅｎｔｒａｔｉｏｎｓ ｏｆ ｔｈｉｏｐｅｎｔａｌ ａｎｄ ｍｉｄａｚｏｌａｍ ｆｏｒ ２４ ｈ， ｗｈｉｌｅ ｔｈｅ ｃｏｎｔｒｏｌ ｇｒｏｕｐ ｗｉｔｈｏｕｔ ａｎｙ ｄｒｕｇｓ． Ｔｈｅｎ ｔｈｅ ｓｔｉｍｕｌａｎｔｓ- ｐｈｏｒｂｏｌｍｙｒｉｓｔａｔｅ＋ｌｏｎｏｍｙｃｉｎ＋ｇｌｏｇｉｓｔｏｐ ｗｅｒｅ ａｄｄｅｄ ｔｏ ｗｈｏｌｅ ｂｌｏｏｄ ａｎｄ ｐｈｙｔｏｈｅｍａｇｇｌｕｔｉｎｉｎ（ＰＨＡ） ｗａｓ ａｄｄ ｔｏ ＰＢＭＣｓ． Ｔｈｅ ｗｈｏｌｅ ｂｌｏｏｄ ｗａｓ ｉｎｃｕｂａｔｅｄ ｆｏｒ ａｎｏｔｈｅｒ ４ ｈ ａｎｄ ＰＢＭＣｓ ｗｅｒｅ ｉｎｃｕｂａｔｅｄ ｆｏｒ ａｎｏｔｈｅｒ ４８ ｈ． Ｔｈｅｎ ｔｈｅ Ｔ-ｌｙｍｐｈｏｃｙｔｅｓ ｗｅｒｅ ｃｏｌｌｅｃｔｅｄ ｆｏｒ ｄｅｔｅｒｍｉｎａｔｉｏｎ ｏｆ ｉｎｔｒａｃｅｌｌｕｌａｒ ｌｅｖｅｌ ｏｆ ＩＦＮ-γ（ａｓ ａ ｍａｒｋｅｒ ｏｆ Ｔｈ１ ｃｅｌｌｓ） ａｎｄ ＩＬ-４（ａｓ ａ ｍａｒｋｅｒ ｏｆ Ｔｈ２ ｃｅｌｌｓ） ｉｎ ｔｈｅ ｗｈｏｌｅ ｂｌｏｏｄ ｕｓｉｎｇ ｆｌｏｗ ｃｙｔｏｍｅｔｒｙ ａｎｄ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ＣＣＲ５＋（ａｓ ａ ｍａｒｋｅｒ ｏｆ Ｔｈ１ ｃｅｌｌｓ） ａｎｄ ＣＣＲ３＋（ａｓ ａ ｍａｒｋｅｒ ｏｆ Ｔｈ２ ｃｅｌｌｓ） ｉｎ ＰＢＭＣｓ． Ｒｅｓｕｌｔｓ：Ｃｏｍｐａｒｅｄ ｗｉｔｈ ｔｈｅ ｃｏｎｔｒｏｌ ｇｒｏｕｐ， ｉｎ ｔｈｉｏｐｅｎｔａｌ ２０ μｇ／ｍｌ， ４０ μｇ／ｍｌ ｇｒｏｕｐ， ｔｈｅ ｐｅｒｃｅｎｔａｇｅ ｏｆ Ｔｈ１ ｃｅｌｌｓ ｄｅｃｒｅａｓｅｄ ｓｉｇｎｉｆｉｃａｎｔｌｙ． Ａｍｏｎｇ ｄｉｆｆｅｒｅｎｔ ｃｏｎｃｅｎｔｒａｔｉｏｎ ｉｎ ｔｈｉｏｐｅｎｔａｌ ｇｒｏｕｐｓ， ｔｈｅ ｐｅｒｃｅｎｔａｇｅ ｏｆ Ｔｈ１ ｃｅｌｌｓ ｉｎ ｈｉｇｈ-ｄｏｓｅ ｏｒ ｍｉｄｄｌｅ-ｄｏｓｅ ｇｒｏｕｐ ｗａｓ ｏｂｖｉｏｕｓｌｙ ｌｏｗｅｒ ｔｈａｎ ｔｈａｔ ｉｎ ｌｏｗ-ｄｏｓｅ ｇｒｏｕｐ， ｔｈｅ ｐｅｒｃｅｎｔａｇｅ ｏｆ Ｔｈ２ ｃｅｌｌｓ ｉｎ ｈｉｇｈ-ｄｏｓｅ ｗａｓ ｏｂｖｉｏｕｓｌｙ ｌｏｗｅｒ ｔｈａｎ ｔｈａｔ ｉｎ ｌｏｗ-ｄｏｓｅ ｇｒｏｕｐ． Ｉｎ ｍｉｄａｚｏｌａｍ ｇｒｏｕｐ， ｔｈｅｒｅ ｗａｓ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｃｈａｎｇｅ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｉｏｐｅｎｔａｌ ｃａｎ ｉｎｈｉｂｉｔ Ｔｈ１ ａｃｔｉｖｉｔｙ ａｎｄ ｓｈｉｆｔ ｔｈｅ ｂａｌａｎｃｅ ｔｏｗａｒｄｓ ｉｍｍｕｎｏｓｕｐｐｒｅｓｓｉｖｅ Ｔｈ２-ｌｉｋｅ ｒｅｓｐｏｎｓｅｓ ｐｒｅｄｏｍｉｎａｎｔｌｙ． Ｍｉｄａｚｏｌａｍ ｈａｓ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｅｆｆｅｃｔ ｏｎ Ｔｈ１ ａｎｄ Ｔｈ２ ａｃｔｉｖｉｔｙ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｅｖａｌｕａｔｅ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｒｅｍｉｆｅｎｔａｎｉｌ ｐｒｅｔｒｅａｔｍｅｎｔ ｏｎ ｐｒｏｐｏｆｏｌ ｉｎｊｅｃｔｉｏｎ ｐａｉｎ． Ｍｅｔｈｏｄｓ：Ａ ｄｏｕｂｌｅ ｂｌｉｎｄ ａｎｄ ｒａｎｄｏｍｉｚｅｄ ｓｔｕｄｙ ｏｆ １５０ ａｄｕｌｔｓ ｕｎｄｅｒｇｏｉｎｇ ｇｅｎｅｒａｌ ａｎｅｓｔｈｅｓｉａ（ＧＡ） ｆｏｒ ｅｌｅｃｔｉｖｅ ｓｕｒｇｅｒｙ ｗａｓ ｃｏｎｄｕｃｔｅｄ． Ａｌｌ ｐａｔｉｅｎｔｓ ｗｅｒｅ ｒａｎｄｏｍｌｙ ａｓｓｉｇｎｅｄ ｔｏ ｏｎｅ ｏｆ ｔｈｅ ｔｈｒｅｅ ｇｒｏｕｐｓ： ｇｒｏｕｐⅠ（ｎ ＝ ５０） ｒｅｃｅｉｖｅｄ １０ ｍｌ ｏｆ ｒｅｍｉｆｅｎｔａｎｉｌ ５０ -滋ｇ ｐｒｅｔｒｅａｔｍｅｎｔ， ｇｒｏｕｐⅡ（ｎ ＝ ５０） ｒｅｃｅｉｖｅｄ ２％ ｌｉｄｏｃａｉｎｅ ４０ ｍｇ ｐｒｅｔｒｅａｔｍｅｎｔ， ａｎｄ ｇｒｏｕｐ Ⅲ ｒｅｃｅｉｖｅｄ ｎｏｒｍａｌ ｓａｌｉｎｅ ｐｒｅｔｒｅａｔｍｅｎｔ ｆｏｌｌｏｗｅｄ ３０ ｓ ｌａｔｅｒ ｂｙ ｐｒｏｐｏｆｏｌ ２ ｍｇ／ｋｇ． Ｄｕｒｉｎｇ ｉｎｄｕｃｔｉｏｎ ｏｆ ＧＡ， ｔｈｅ ｐａｉｎ ｒｅｓｐｏｎｓｅ ｗａｓ ｒｅｃｏｒｄｅｄ． Ｈｅｍｏｄｙｎａｍｉｃ ｄａｔａ ｗｅｒｅ ａｌｓｏ ｒｅｃｏｒｄｅｄ． Ｒｅｓｕｌｔｓ：Ｔｈｅ ｉｎｃｉｄｅｎｃｅ ａｎｄ ｉｎｔｅｎｓｉｔｙ ｏｆ ｉｎｊｅｃｔｉｏｎ ｐａｉｎ ｗａｓ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｌｅｓｓ ｉｎ ｔｈｅ ｇｒｏｕｐ ⅠａｎｄⅡ ｔｈａｎ ｔｈｏｓｅ ｉｎ ｔｈｅ ｇｒｏｕｐ Ⅲ（Ｐ ＜ ０．０５）， ａｎｄ ｔｈｅ ｈｅｍｏｄｙｎａｍｉｃ ｄａｔａ ｗｅｒｅ ｎｏｔ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｃｈａｎｇｅｄ（Ｐ ＞ ０．０５）． Ｃｏｎｃｌｕｓｉｏｎ：Ｐｒｏｐｏｆｏｌ-ｉｎｄｕｃｅｄ ｐａｉｎ ｃａｎ ｂｅ ｒｅｄｕｃｅｄ ｂｙ ｒｅｍｉｆｅｎｔａｎｉｌ ｐｒｅｔｒｅａｔｍｅｎｔ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｃｌｉｎｉｃａｌ ｃｈａｒａｃｔｅｒｉｓｔｉｃｓ ａｎｄ ｔｒｅａｔｍｅｎｔ ｏｆ ｏｖａｒｉａｎ ｈｙｐｅｒｓｔｉｍｕｌａｔｉｏｎ ｓｙｎｄｒｏｍｅ（ＯＨＳＳ）． Ｍｅｔｈｏｄｓ：Ｃｌｉｎｉｃａｌ ｄａｔａ ｏｆ ７０ ＯＨＳＳ ｐａｔｉｅｎｔｓ ｗｅｒｅ ａｎａｌｙｚｅｄ ｒｅｔｒｏｓｐｅｃｔｉｖｅｌｙ． Ｒｅｓｕｌｔｓ：ＯＨＳＳ ｍａｉｎｌｙ ｏｃｃｕｒｒｅｄ ａｆｔｅｒ ｐｒｏ-ｏｖｕｌａｔｉｏｎ ｔｒｅａｔｍｅｎｔ． Ａｂｄｏｍｉｎａｌ ｄｉｓｔｅｎｓｉｏｎ，ｎａｕｓｅａ，ａｓｃｉｔｅｓ，ｅｄｅｍａ，ｏｌｉｇｒｉａ，ｃｏｎｃｅｎｔｒａｔｅｄ ｂｌｏｏｄ，ｈｙｐｏａｌｂｕｍｉｎｅｍｉａ ｗｅｒｅ ｔｈｅ ｍａｊｏｒ ｍａｎｉｆｅｓｔａｔｉｏｎｓ ｏｆ ＯＨＳＳ，ｗｈｉｃｈ ｃｏｕｌｄ ｂｅ ｃｏｎｔｒｏｌｌｅｄ ｂｙ ｉｎｔｅｎｓｉｖｅ ｃａｒｅ，ｓｕｐｐｌｅｍｅｎｔａｔｉｏｎ ｏｆ ａｌｂｕｒｍｉｎ ｏｒ ｐｌａｓｍａ，ｖｏｌｕｍｅ ｅｘｐａｎｓｉｏｎ，ａｎｄ ｐａｒａｃｅｎｔｅｓｉｓ． Ｃｏｎｃｌｕｓｉｏｎ：Ｇｒｅａｔ ａｔｔｅｎｔｉｏｎ ｓｈｏｕｌｄ ｂｅ ｐａｉｄ ｔｏ ｐｒｅｖｅｎｔｉｏｎ ＯＨＳＳ ｗｈｅｎ ｅｎｈａｎｃｉｎｇ ｏｖｕｌａｔｉｏｎ． Ｉｎｔｅｎｓｉｖｅ ｃａｒｅ ｉｓ ｎｅｃｅｓｓａｒｙ ｉｎ ｔｈｅ ｔｒｅａｔｍｅｎｔ ｏｆ ｍｏｄｅｒａｔｅ ａｎｄ ｓｅｖｅｒｅ ＯＨＳＳ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｅｖａｌｕａｔｅ ｐｏｓｔｏｐｅｒａｔｉｖｅ ｃｏｒｎｅａｌ ｓｗｅｌｌｉｎｇ ａｓ ａ ｐｒｅｄｉｃｔｏｒ ｏｆ ｃｏｒｎｅａｌ ｅｎｄｏｔｈｅｌｉａｌ ｃｅｌｌ ｌｏｓｓ ａｆｔｅｒ ｐｈａｃｏｅｍｕｌｓｉｆｉｃａｔｉｏｎ ｃａｔａｒａｃｔ ｓｕｒｇｅｒｙ． Ｍｅｔｈｏｄｓ：Ｔｈｉｓ ｓｔｕｄｙ ｉｎｃｌｕｄｅｄ ４２８ ｅｙｅｓ ｗｈｉｃｈ ｗｅｒｅ ｐｅｒｆｏｒｍｅｄ ｒｏｕｔｉｎｅ ｐｈａｃｏｅｍｕｌｓｉｆｉｃａｔｉｏｎ ｓｕｒｇｅｒｙ． Ｕｌｔｒａｓｏｎｉｃ ｐａｃｈｙｍｅｔｒｙ ａｎｄ ｅｎｄｏｔｈｅｌｉａｌ ｐｈｏｔｏｇｒａｐｈｙ ｏｆ ｔｈｅ ｃｅｎｔｒａｌ ｏｆ ｔｈｅ ｃｏｒｎｅａ ａｎｄ Ｏｒｂｓｃａｎ ＩＩ ｓｌｉｔ-ｓｃａｎ ｔｏｍｏｇｒａｐｈｙ ｗｅｒｅ ｐｅｒｆｏｒｍｅｄ ｐｒｅｏｐｅｒａｔｉｖｅｌｙ， ｏｎ ｔｈｅ ｔｈｅ ｆｉｒｓｔ ｄａｙ ａｆｔｅｒ ｓｕｒｇｅｒｙ ａｎｄ ｔｈｒｅｅ ｍｏｎｔｈｓ ａｆｔｅｒ ｓｕｒｇｅｒｙ． ＳＰＳＳ １３．０ ｗａｓ ｕｓｅｄ ｆｏｒ ｓｔａｔｉｓｔｉｃａｌ ｃａｌｃｕｌａｔｉｏｎｓ． Ｉｎｄｅｐｅｎｄｅｎｔ-Ｓａｍｐｌｅｓ ｔ ｔｅｓｔ ａｎｄ ｒｅｐｅａｔｅｄ ｍｅａｓｕｒｅｓ ｗｅｒｅ ｕｓｅｄ ｔｏ ｔｅｓｔ ｄｉｆｆｅｒｅｎｃｅｓ ａｎｄ ａ ｂａｃｋｗａｒｄ ｓｔｅｐｗｉｓｅ ｍｕｌｔｉｐｌｅ ｒｅｇｒｅｓｓｉｏｎ ａｎａｌｙｓｉｓ ｗｅｒｅ ｐｅｒｆｏｒｍｅｄ ｔｏ ｉｎｄｅｎｔｉｆｙ ｔｈｅ ｖａｒｉａｂｌｅｓ ｉｎｄｅｐｅｎｄｅｎｔｌｙ ｃｏｎｔｒｉｂｕｔｉｎｇ ｔｏ ｔｈｅ ｃｏｒｎｅａｌ ｅｎｄｏｔｈｅｌｉａｌ ｃｅｌｌ ｌｏｓｓ． Ｒｅｓｕｌｔｓ：Ｔｈｅ ｒａｔｉｏ ｏｆ ｅｎｄｏｔｈｅｌｉａ ｃｅｌｌ ｌｏｓｓ ３ ｍｏｎｔｈｓ ａｆｔｅｒ ｐｈａｃｏｅｍｕｌｓｉｆｉｃａｔｉｏｎ ｃａｔａｒａｃｔ ｓｕｒｇｅｒｙ ｗａｓ １８．３４ ± １１．６３％ ｆｒｏｍ １．５６％ ｔｏ ５９．３０％ ｉｎ ｔｈｉｓ ｓｔｕｄｙ． Ｉｔ ｗａｓ ｃｌｏｓｅｌｙ ｒｅｌａｔｅｄ ｔｏ ｐｈａｃｏ ｔｉｍｅ， ｎｕｃｌｅａｒ ｈａｒｄｎｅｓｓ ａｎｄ ｃｏｒｎｅａｌ ｐａｃｈｙｍｅｔｒｙ ｃｈａｎｇ ｔｏ ｆｉｒｓｔ ｄａｙ ａｆｔｅｒ ｓｕｒｇｅｒｙ． Ｃｏｎｃｌｕｓｉｏｎ：Ｉｎ ｔｈｉｓ ｓｔｕｄｙ， ｔｈｅ ｄｅｇｒｅｅ ｏｆ ｐｅｒｍａｎｅｎｔ ｃｏｒｎｅａｌ ｅｎｄｏｔｈｅｌｉａｌ ｄａｍａｇｅ ｈａｄ ｓｉｇｎｉｆｉｃａｎｔ ｃｏｒｒｅｌａｔｉｏｎ ｗｉｔｈ ｔｈｅ ｄｅｇｒｅｅ ｏｆ ｅａｒｌｙ ｐｏｓｔｏｐｅｒａｔｉｖｅ ｃｏｒｎｅａｌ ｓｗｅｌｌｉｎｇ． Ｍｅａｓｕｒｉｎｇ ｔｈｅ ｄｉｆｆｅｒｅｎｃｅ ｉｎ ｐｈａｃｈｙｍｅｔｒｙ ａｔ ｐｏｓｔｏｐｅｒａｔｉｖｅ ｆｉｒｓｔ ｄａｙ ｉｓ ａ ｕｓｅｆｕｌ ｗａｙ ｔｏ ａｓｓｅｓｓ ｔｈｅ ｅｆｆｅｃｔｓ ｏｎ ｔｈｅ ｃｏｒｎｅａｌ ｅｎｄｏｔｈｅｌｉｕｍ ｅｘｅｒｔｅｄ ｂｙ ｔｈｅ ｐｈａｃｏｅｍｕｌｓｉｆｉｃａｔｉｏｎ ｐｒｏｃｅｄｕｒｅ．

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｉｎｖｅｓｔｉｇａｔｅ ｔｈｅ ｌｅｖｅｌｓ ｏｆ ｂｌｏｏｄ ｐｌａｓｍａ ｅｓｔｒａｄｉｏｌ（Ｅ２） ａｎｄ ｔｅｓｔｏｓｔｅｒｏｎｅ（Ｔ） ｉｎ ｎｅｏｎａｔａｌ ｈｙｐｏｘｉｃ-ｉｓｃｈｅｍｉｃ ｅｎｃｅｐｈａｌｏｐａｔｈｙ（ＨＩＥ） ａｎｄ ｅｘｐｌｏｒｅ ｔｈｅ ｒｅｌａｔｉｏｎ ｏｆ ｂｌｏｏｄ ｐｌａｓｍａ Ｅ２ ａｎｄ Ｔ ｗｉｔｈ ｔｈｅ ｄｅｖｅｌｏｐｉｎｇ ｏｆ ＨＩＥ， ａｎｄ ｔｏ ｓｕｐｐｌｙ ｇｕｉｄａｎｃｅ ｆｏｒ ｄｉａｇｎｏｓｅ ａｎｄ ｔｈｅｒａｐｙ ｏｆ ＨＩＥ． Ｍｅｔｈｏｄｓ：Ｂｌｏｏｄ ｐｌａｓｍａ Ｅ２ ａｎｄ Ｔ ｌｅｖｅｌｓ ｗｅｒｅ ｍｅａｓｕｒｅｄ ｂｙ ｅｌｅｃｔｒｏｃｈｅｍｉｌｕｍｉｎｅｓｃｅｎｃｅ ｉｎ ５７ ｎｅｏｎａｔｅｓ ｗｉｔｈ ＨＩＥ（２１ ｍｉｌｄ，２１ ｍｏｄｅｒａｔｅ，１５ ｓｅｖｅｒｅ ＨＩＥ） ａｔ ｔｈｅ ａｃｕｔｅ ａｎｄ ｃｏｎｖａｌｅｓｃｅｎｃｅ ｓｔａｇｅｓ． Ｔｈｉｒｔｙ ｎｅｏｎａｔｅｓ ｗｅｒｅ ｕｓｅｄ ａｓ ｃｏｎｔｒｏｌ． Ｒｅｓｕｌｔｓ：Ｂｌｏｏｄ ｐｌａｓｍａ Ｅ２ ａｎｄ Ｔ ｗｅｒｅ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｈｉｇｈｅｒ ａｔ ａｃｕｔｅ ｓｔａｇｅ ｂｕｔ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｌｏｗｅｒ ａｔ ｃｏｎｖａｌｅｓｃｅｎｃｅ ｓｔａｇｅ ｉｎ ｍｏｄｅｒａｔｅ ａｎｄ ｓｅｖｅｒｅ ＨＩＥ ｎｅｏｎａｔｅｓ ｃｏｍｐａｒｅｄ ｗｉｔｈ ｔｈｏｓｅ ｏｆ ｃｏｎｔｒｏｌ ａｎｄ ｍｉｌｄ ＨＩＥ ｎｅｏｎａｔｅｓ． Ｂｌｏｏｄ ｐｌａｓｍａ Ｅ２ ａｎｄ Ｔ ｄｅｃｒｅａｓｅｄ ｉｎ ｍｏｄｅｒａｔｅ ａｎｄ ｓｅｖｅｒｅ ＨＩＥ ｎｅｏｎａｔｅｓ ａｔ ｃｏｎｖａｌｅｓｃｅｎｃｅ ｓｔａｇｅ ｃｏｍｐａｒｅｄ ｗｉｔｈ ｔｈｏｓｅ ａｔ ｔｈｅ ａｃｕｔｅ ｓｔａｇｅ． Ｔｈｅ ｄｉｖｅｒｓｉｔｙ ｏｆ ｂｌｏｏｄ ｐｌａｓｍａ Ｅ２ ａｎｄ Ｔ ｗｅｒｅ ｕｎｉｆｉｃａｔｉｏｎ ｗｉｔｈ ｔｈｅ ｌｅｖｅｌｓ ｏｆ ＮＢＮＡ． Ｃｏｎｃｌｕｓｉｏｎ：Ｅ２ ａｎｄ Ｔ ｍｉｇｈｔ ｂｅ ｉｎｖｏｌｖｅｄ ｉｎ ｔｈｅ ｐａｔｈｏｐｈｙｓｉｏｌｏｇｉｃａｌ ｐｒｏｃｅｓｓ ｏｆ ＨＩＥ． Ｔｈｅ ｌｅｖｅｌｓ ｏｆ Ｅ２ ａｎｄ Ｔ ｃｌｏｓｅｌｙ ｒｅｌａｔｅ ｔｏ ｔｈｅ ｓｅｖｅｒｉｔｙ ｏｆ ＨＩＥ．

Abstract:Objective：To study how to model zero-inflated count data, and apply it to handle the data about respiratory infection. Methods：Zero-inflated model with 2 parts，zero and Poisson distribution, were transformed to conditional zero-inflated model. Logit and Log linkage function were used to promote the explanation of the result. Results：The results of significant risk factors were those who were younger and live lower in the ZTP part; the risk factors were younger exerciser, having history of chronic respiratory system disease and ill body conditions in the LOGIT part. Conclusion：The conditional zero-inflated model could be easier to explain the affected factors.

Abstract:Ｏｂｊｅｃｔｉｖｅ：Ｔｏ ｕｎｄｅｒｓｔａｎｄ ｋｎｏｗｌｅｄｇｅ，ａｔｔｉｔｕｄｅｓ，ｈｉｇｈ ｒｉｓｋ ｂｅｈａｖｉｏｒ ｒｅｌａｔｅｄ ｔｏ ＨＩＶ／ＳＹＰＨＩＬＩＳ ａｎｄ ｔｈｅ ｉｎｆｅｃｔｉｏｎ ｒａｔｅ ｏｆ ＨＩＶ／ＳＹＰＨＩＬＩＳ ａｍｏｎｇ ｍｅｎ ｈａｖｅ ｓｅｘ ｗｉｔｈ ｍｅｎ（ＭＳＭｓ）， ａｎｄ ｔｏ ｐｒｏｖｉｄｅ ｔｈｅ ｅｆｆｅｃｔｉｖｅ ｉｎｆｏｒｍａｔｉｏｎ ａｎｄ ｅｖｉｄｅｎｃｅ ｆｏｒ ｐｒｅｖｅｎｔｉｏｎ ｉｎｔｅｒｖｅｎｔｉｏｎ． Ｍｅｔｈｏｄｓ：Ａ ａｎｏｎｙｍｏｕｓ ｉｎｖｅｓｔｉｇａｔｉｏｎ ｗａｓ ａｐｐｌｉｅｄ ｉｎ ｔａｒｇｅｔ ｏｂｊｅｃｔ ｉｎ ｔｈｅ ｂａｌｎｅａｒｙ ｗｈｅｒｅ ＭＳＭ ｍａｓｓｅｄ， ａｎｄ ｔｈｅｉｒ ｂｌｏｏｄ ｗｅｒｅ ｇａｔｈｅｒｅｄ ｔｏ ｔａｋｅ ＨＩＶ／ＳＹＰＨＩＬＩＳ ｔｅｓｔｓ． Ｒｅｓｕｌｔｓ：Ｔｈｅｉｒ ｉｎｔｅｇｒａｔｅｄ ａｗａｒｅｎｅｓｓ ｒａｔｅ ｏｆ ＨＩＶ ｗａｓ ７５．３％． Ｏｎｌｙ ２１．６％ ＭＳＭ ｕｓｅｄ ｃｏｎｄｏｍ ｗｈｅｎ ｔｈｅｙ ｈａｄ ａｎａｌ ｓｅｘ ｗｉｔｈ ｓａｍｅ ｓｅｘ， ７８．４％ ＭＳＭ ｎｅｖｅｒ ｕｓｅｄ ｏｒ ｓｏｍｅｔｉｍｅｓ ｕｓｅｄ． １１７ ＭＳＭｓ ｗｈｏ ｈａｄ ｓｅｘ ｂｅｈａｖｉｏｒ ｗｉｔｈ ｆｅｍａｌｅ ｐａｒｔｎｅｒｓ ｗｉｔｈｉｎ ｒｅｃｅｎｔ ３ ｍｏｎｔｈｓ， ａｎｄ ｒａｔｅ ｏｆ ｕｓｉｎｇ ｃｏｎｄｏｍ ｗａｓ ｏｎｌｙ １９．７％． Ｔｈｅ ｉｎｆｅｃｔｉｏｎ ｏｆ ＨＩＶ，ＴＰＰＡ，ＲＰＲ，ｗａｓ ４．７％，３９．９％，２７．０％． Ｔｈｅ ｃｏｒｒｅｌａｔｉｏｎ ｂｅｔｗｅｅｎ ＨＩＶ／ＳＹＰＨＩＬＩＳ ｉｎｆｅｃｔｉｏｎ ａｎｄ ａｇｅ，ｎｕｍｂｅｒ ｏｆ ａｎａｌ ｓｅｘ ｐａｒｔｎｅｒｓ ｗｉｔｈｉｎ ３ ｍｏｎｔｈｓ，ｍａｒｒｉａｇｅ ｓｔａｔｕｓ，ｅｄｕｃａｔｉｏｎ，ｕｓｉｎｇ ｏｆ ｃｏｎｄｏｍ，ｎａｔｉｖｅ ｐｌａｃｅ ｗａｓ ｉｎｓｉｇｎｉｆｉｃａｎｔ． Ｃｏｎｃｌｕｓｉｏｎ：Ｔｈｅ ｈｉｇｈ ｒｉｓｋ ｓｅｘｕａｌ ｂｅｈａｖｉｏｒｓ ａｒｅ ｃｏｍｍｏｎ ｓｅｅｍ ａｍｏｎｇ ｔｈｅ ＭＳＭｓ ｉｎ ｂａｌｎｅａｒｙ，ａｎｄ ｔｈｅ ｉｎｆｅｃｔｉｏｎ ｏｆ ＨＩＶ／ＳＹＰＨＩＬＩＳ ｉｎ ＭＳＭｓ ｗｅｒｅ ｑｕｉｔｅ ｈｉｇｈ． Ｓａｆｅ ｓｅｘ ｉｎｖｏｌｖｅｄ ｂｅｈａｖｉｏｒ ｉｎｔｅｒｖｅｎｔｉｏｎ ｐｒｏｇｒａｍｓ ｓｈｏｕｌｄ ｂｅ ｅｆｆｅｃｔｉｖｅｌｙ ｃａｒｒｉｅｄ ｏｕｔ ａｓ ｗｅｌｌ ａｓ ｓｔｒｅｎｇｔｈｅｎｅｄ ｈｅａｌｔｈ ｅｄｕｃａｔｉｏｎ ｐｒｏｇｒａｍｓ ｔｏ ＭＳＭｓ ｉｎ ｏｒｄｅｒ ｔｏ ｃｏｎｔｒｏｌ ｔｈｅ ｒａｐｉｄ ｓｐｅａｄ ｏｆ ＨＩＶ／ＳＴＤｓ．