Abstract:Objective:To study nuclear factor-κB(NF-κB) activation in heart failure due to chronic pressure-overload. Methods: SD rats were performed by transverse aortic banding for 12 weeks. After that hemodynamic parameters and cardiac fibrosis were measured;NF-κB binding activity was analyzed by electrophoretic mobility shift assay(EMSA);Immunoprecipitation(IP) was performed to analysis Toll-like receptor 4(TLR4) and myeloid differentiation primary-response protein 8(MyD88) association. Results:Both systolic and diastolic function were significantly reduced compared with sham operated group(P < 0.05), and cardiac fibrosis enhanced predominantly. NF-κB binding activity in hypertrophic heart tissue was higher than that of sham operated group(P < 0.05) as well as TLR4/MyD88 association(P < 0.05). Conclusion:NF-κB was required for chronic pressure overload-induced heart failure.

Abstract:Objective:To investigate the changes of zinc finger protein 207(ZNF207)gene mRNA during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between ZNF207 gene level and the differentiation stage of cardiac myocytes. Methods:P19 cells were cultured and induced by 0.9%DMSO to differentiate to cardiac myocytes in vitro. Total RNA were extracted from P19 cells during the process of differentiation at various time points:and the levels of ZNF207 mRNA were evaluated by RT-PCR. Results:When P19 cells were induced by DMSO to differentiate to cardiac myocytes, the expression level of ZNF207 gene mRNA was up-regulated and reached the highest level at 10 th day. There was a significant difference between day 2 to day 10(P < 0.05). Conclusion:The expression of ZNF207 gene was up-regulated in the process of the differentiation of P19 cells to cardiac myocytes,which might be involved in the differentiation of cardiac myocytes.

Abstract:Objective:To investigate the effect of myocardial ischemia-reperfusion on the expression of transcription factor GATA-4. Methods:Sixteen adult healthy Balb/c mice were randomly divided into control group and experimental group. Heart ischemia was achieved by ligation of left anterior coronary descending branch. After 45 min ischemia, the ligation was removed for 4 h reperfusion. Echocardiography was used to determine the heart function. Heart samples were taken for further analysis. Results:I/R resulted in poor heart function, accompanying the downregulation of GATA-4 mRNA and protein expression. Histological examination showed there were more inflammatory cells in I/R heart. Conclusion:GATA-4 was modulated by I/R and probably responsible for I/R induced heart injury partially.

Abstract:Objective:To assess the effect of G894T polymorphism in exon 7 of the endothelial nitric oxide synthase gene(eNOS)on the development of acute coronary syndromes(ACS). Methods:By means of Polymerase Chain Reaction and Restriction Fragment Length Polymorphism,G894T Polymorphism of eNOS was detected in 260 patients with ACS(120 with acute MI and 140 with unstable angina) and 248 subjects excluded CHD by coronary angiography. Results:Genotypes GT+TT and T allele frequency in ACS group were significant higher than those in control group(23.46%vs 9.27%,14.42%vs 5.24%)(P < 0.01). There was no significant difference in genotypes GT+TT between AMI subgroup and UAP subgroup(20.83% vs 25.71%,P > 0.05). That did not correlate with the number of coronary vessels diseased in patients with ACS. Conclusion:The T allele of eNOS gene might be associated with ACS and a risk genetic marker of CHD evolution in ACS. But that is not related to the severity of coronary diseased.

Abstract:Objective:To study the protein expression about the HIF-1α,ERK and p-ERK on ischemic myocardium after acute myocardiac infarction(AMI) and to explore initially the mechanism about HIF-1α. Methods:Rabbits AMI model were establised, and divided into 4 groups at random, and infected with Ad-HIF-1α,Ad-Blank,Rz-HIF-1α respectively. Sham group was regarded as control. The protein expression of HIF-1α,ERK and p-ERK was detected by immunohistochemistry and Western-blot. Results:The protein expression of exogenous HIF-1α and p-ERK increased at 1d after AMI, reached the peak at 7d and decreased gradually at 14d and 28d. At 56d,the protein of HIF-1α and p-ERK was not expressed. Among four groups, there was no difference in the protein level of ERK,while p-ERK protein expression was higher in group Ad-Blank than that in group Sham, lower than that in group Ad-HIF-1α,and the protein level of p-ERK was the lowest in group Rz-HIF-1α. Conclusion:Ad-HIF-1α was transducted effectively and promote ERK phosphorylated. HIF-1α ribozyme gene can block the protective effect of HIF-1α on the ischemic myocardium.

Abstract:Objective:The aim of this study was to assess the expression of protein products of CBP(CSK-binding protein) in non-small cell lung cancer(NSCLC), to estimate their possible expression and to correlate the results of immunohistochemical analysis with various clinicopathologic parameters. Methods:The expression of CBP gene was detected by immunohistochemical S-P method in 50 cases of NSCLC tissues,50 para-cancer tissues and 10 cases of lung benign lesion tissues as control. According to immunohistochemical results, the expressions of CBP mRNA were detected by reverse transcriptase polymerase chain reaction(RT-PCR) in corresponding samples. Results:The expression level of CBP gene was found to be significantly lower in NSCLC tissues than in para-cancer tissues and lung benign lesion tissues(P < 0.05). The expression level of CBP gene was closely related to PTNM stages and lymph node involvement in Lung cancer patients(P < 0.05). Conclusion:CBP gene may play a role in the negative feedback regulation of Src-family kinases in non-small cell lung cancer, and reduction of CBP gene expression may be related to the pathogenesis and development of NSCLC.

Abstract:Objective:To explore the expression of osteopontin(OPN), CD44v6 in human pancreatic carcinomas and analyze the relationship between their expression and clinicopathologic features. Methods:The expression of OPN,CD44v6 was tested by immunohistochemistry in 43 cases of pancreatic carcinomas,34 cases of pancreatic paraneoplastic tissues. Results:In 43 cases of pancreatic carcinomas, the expression rates of OPN,CD44v6 were 65.1%,62.7% respectively;in 34 cases of pancreatic paraneoplastic tissues, the expression rates of OPN and CD44v6 were 20.6% both. OPN and CD44v6 expression was higher in carcinomas with lymph node metastases than that without metastases; CD44v6 expression was higher in carcinomas with histological grade Ⅲ(P < 0.05) than that with Ⅰ~Ⅱ grade, and there were strong correlation between OPN and CD44v6(r = 0.446). Conclusion:The expressions of OPN and CD44v6 were higher in pancreatic carcinomas. They might play an important role in the progression of human pancreatic carcinomas and participat in the invasion and metastasis of pancreatic carcinomas.

Abstract:Objective:To investigate the expression of c-Fos and c-Jun in AGS cells by stimulation of H. pylori. Methods:The gastric cancer AGS cells were treated with the different types of H. pylori(isolated from gastritis, ulcer or gastric cancer) for one hour, respectively. The lysate of the cells were collected to detect the expression of the two genes by Western blot. Results:The expression of c-Fos protein in the cells stimulated by ulcer type and that of c-Jun by gastritis type increased obviously. Furthermore, the expression of proteins of c-Fos and c-Jun by the stimulation of gastritis and ulcer types mixed was more increased than that of single type of H. pylori and so was the expressions of c-Fos and c-Jun by stimulation of the mixture of the three types. However, there were unremarkable expression changes of the two proteins in the cells treated only with the type of gastric cancer. Conclusion:There was difference in the proto-oncogene c-fos and c-jun expression in AGS cells stimulated with different types of H. pylori. The expression of the two genes was more sensitive in the AGS cells treated with multi types of H. pylori. It indicated that the cancer occurrence was promotered by mixed infection of multi types of H. pylori.

Abstract:Objective:To investigate the growth effect of breast cancer cells by insulin. Methods:MCF-7 cells were exposed to the insulin at various concentrations(25,50,100,200 nmol/L) for 24, 48 and 72 h. The effect of insulin on cell proliferation was assessed by morphologic observing and colorimetric MTT methods. Results:When exposed to the insulin at 200 nmol/L for 72 h, the modality of MCF-7 cells had a significant change. The villus in micro lacuna of cytoplast decreased and mitochondrion expanded obviously. Insulin at different concentrations can significantly promote the cell proliferation, especially at the concentrations of 50, 100 and 200 nmol/L insulin for times of 24,48 and 72 h(P < 0.05 or P < 0.01). And it was showed that this effect was dose- and time- dependent. Conclusion:Insulin can stimulate the proliferation of breast cancer cells in a dose-and time-dependent way. It was indicated that the insulin was a factor to stimulate the proliferation of MCF-7 cells line.

Abstract:Objective:To explore the expression and significance of WWOX and FHIT in colorectal cancer. Methods:The expression of WWOX and FHIT was evaluated by immunohistochemical staining(two-step method) in 75 colorectal carcinoma specimens and 30 normal paraneoplastic colorectal tissues,and their correlation with clinicopathological features was analyzed. Results:The rate of WWOX and FHIT positive expression in tumors was 52% and 54.7%,respectively,which was significantly different from that in normal paraneoplastic colorectal tissues as 93.3% and 97.8%. WWOX and FHIT expression were correlated strongly(P < 0.05). The expression of WWOX and FHIT was closely associated with histological grade,Duke stage and 5-year survival rate. And the expression of wwox and FHIT had no significant correlation with neoplastic site and ages. There was a correlation between WWOX expression and FHIT This correlation was consistent with the susceptibility of common fragile sites to DNA damage. Conclusion:The positive expression of WWOX and FHIT was lower than the normal tissues and closely associated with histological grade,Duke stage,and 5-year survival rate(P < 0.05). WWOX and FHIT expression were correlated strongly(P < 0.05). It is suggested that WWOX and FHIT genes may play an important role in the initiation and development of colorectal cancer. They can become targets of predicting the progression of colorectal cancer.

Abstract:Objective:To detect the effect of PDTC,the selective inhibitor of NF-κB,on the growth and apoptosis of human gastric carcinoma cell SGC-7901,to analyze the expression of Livin and Caspase-3 of cell SGC-7901treated by PDTC,and to explore the mechanism of PDTC in induction of tumor cell-蒺s apoptosis. Methods:After the treatment with PDTC on human gastric carcinoma cell SGC-7901,MTT assay was used to observe the growth inhibition of cell SGC-7901.The cell apoptosis was assessed by flow cytometry (FCM). The mRNA and protein expressions of Livin and Caspase-3 were detected by real time PCR and Western blot assay,respectively. Results:When PDTC was given,growth inhibition and elevated apoptosis of SGC-7901 cells were detected,which was showed in a dose- and time- dependent manner. PDTC also down-regulated the level of mRNA and protein expression of Livin,and elevated the mRNA and protein expression of Capase-3. Conclusion:PDTC can induce apoptosis of human gastric carcinoma cell SGC-7901 cells,which may be performed by down-regulating of Livin and up-regulating of Caspase-3 expression.

Abstract:Objective:To explore the inhibition of different chemotherapeutic drugs to the breast cancer T47D cell line which highly expresses γ-synuclein(SNCG). Methods:T47D with SNCG expression and MCF-7 without SNCG expression cell lines were routinely cultured. MTT method was employed to evaluate the inhibiting roles of 6 chemotherapeutic drugs(DDP,ADM,5-FU,VCR,TAX and CPT-11) to the two cell lines. Moreover, Flow cytometry was enrolled to analyze the cell cycles and the apoptosis of T47D cell lines. Results:The inhibiting roles of ADM,VCR,TAX and CPT-11 to T47D were much lower than that of MCF-7 cell line after 60 h treatment(P < 0.01), while the effects of 5-FU and DDP to T47D cell line were not only the same as to MCF-7, but also much higher than the other 4 drugs(P < 0.01). Moreover, there were significant differences of PI and caspase-3 expressions of T47D cell line between DDP,5-FU groups and control or other 4 groups, respectively(P < 0.01). Conclusion:The inhibiting effects of DDP,5-FU to T47D cell line with high expression of SNCG are much higher than those of ADM,VCR,TAX and CPT-11,which can provide experimental evidence in vitro for the chemotherapy strategy to breast cancer patients.

Abstract:Objective:To construct the fusion gene of human hTERT and human heat shock protein 70(HSP70), and expressed it in E.coli. Methods:The hTERT and HSP70 genes were amplified by PCR. The hTERT gene was digested by NcoⅠand EcoRⅠ enzyme and inserted into the plasmid pET32a to construct pET32a-hTERT plasmid. Then the HSP70 gene was cloned into the expression vector pET32a-hTERT between EcoRⅠ and HindⅢ site to construct the prokaryotic expression plasmid pET32a-hTERT-HSP70. The E.coli BL21 contained the plasmid was induced by IPTG. Results:The HSP70 gene and hTERT gene segments were amplified from their templates; the DNA sequencing result showed that the prokaryotic fusion expression plasmid pET32a-hTERT-HSP70 was successfully constructed. The E.coli BL21 contained the plasmid could express a Mr 110 kD protein after induced by IPTG. Conclusion:The successful expression of fusion protein of human hTERT and heat shock protein 70 enables the further research of the HSP70 protein as a adjuvant-free carrier.

Abstract:Objective:To construct an eukaryotic expression recombinant plasmid pEGFP-N1-PLZF and get its protein translation in spermatogonia of the neonatal rats in vitro. Methods:Using the primers of PLZF gene and the total RNA extracted from the tissue of rat testes, RT-PCR was performed. The product was inserted to multiple clone sites of EGFP-N1 vector by DNA recombinant technique. Then a new eukaryotic expression recombinant plasmid pEGFP-N1-PLZF was generated and identified by incision enzyme EcoRⅠ and SalⅠ and DNA sequencing. pEGFP-N1-PLZF was transfected into spermatogonia by liposome, from which protein were extracted after 24 h for detecting their expression by Western blot analysis. The treated cells were continuously traced by fluorescence microscope. Results:The recombinant plasmid cut by incision enzyme EcoRⅠ and SalⅠ overnight generated a 2 kb fragment,and DNA sequence of the 2 kb fragment was identical with rat plzf mRNA in genebank. In the cells 24 h after transfected with recombinant plasmid, Western blot analysis showed a 106 kD fusion protein. Green fluorescence could be seen by fluorescence microscope after 24 h. Conclusion:The recombinant plasmid was successfully constructed and was a useful tool to study the proliferation and differention of spermatogonia.

Abstract:Objective:To investigate the difference between the sera of the hepatitis B vaccine inoculators and the recovered patients with acute infection in blocking hepatitis B virus infection in vitro. Methods:Titres of anti-HBs were tested by ELISA using Abbott kit in the sera of the vaccinates and the recovered patients with acute hepatitis B virus infection. Hep G2 cells were infected by wild type or “a” determinant mutant of HBV with or without pre-incubation for 1 h by the sera of the vaccinates(group 1) and the recovered patients with acute hepatitis B virus infection(group 2). 24 h later HBV DNA in Hep G2 cells were detected by PCR. The neutralization effect was determined based on the PCR results. Results:There is no significant difference between group 1 and group 2 in blocking wild type of hepatitis B virus infection to Hep G2 at the same concentration in vitro. The sera of group 2 can block the “a” determinant mutant strain HBV infection to Hep G2 cells, but the neutralization effect was weaker than that on wild type of HBV infection. The sera of group 2 have no neutralization effect on the “a” determinant mutant strain HBV infection. Conclusion:The sera of recovered patients with HBV acute infection can block both wild strain and “a” determinant mutant strain of HBV infection, while the sera of hepatitis B vaccine inoculators have no neutralizing effect on “a” determinant mutant strain. It suggested that the new hepatitis B vaccine should include more antigen component such as preS1/S2 to develop neutralization effect on “a” determinant mutant strain HBV, and more component anti-HBV antibodies was very important to protect HBV infected patients from mutant HBV reinfection after liver transplantation.

Abstract:Objective:To prepare and identify the monoclonal antibody(mAb) against protease activated receptor-2(PAR-2). Methods:BLAB/c mice were immunized with specific PAR-2 polypeptide by multiple-site hypodermic injection. The spleen cells of immunized mice were fused with NS-1 myeloma cells. Indirect ELISA was used to screen the hybridoma culture supernants. The specificity of mAb were characterized by ELISA,Dot blot,immunohistochemistry,flow cytometry and confocal laser scanning microscopy(CLSM). Results:One hybridoma cell line against PAR-2 was successfully obtained, this mAb was IgM type and the titer of antibody in supernants was 1 ∶ 16. Dot blot analysis showed that the mAb could specifically bind to PAR-2. Immunohistochemical staining revealed that the reactivity of mAb to epithelial cells and smooth muscle cells in lung, epithelial cells and lymphocyte-like cells in colon, lymphocytes in tonsil and squamous epithelial cells in skin tissue. The result of flow cytometry showed that the mAb recognized PAR-2 in A549 cells. CLSM examination confirmed that the fluorescent markers were localized in both cytomembrane and cytoplasm of A549 cells. Conclusion:The mAb against PAR-2 is a useful tool for investigate PAR-2 related disease

Abstract:Objective:To establish lung perfusion model of rabbit in vivo. and to investigate the isolated lung preservation effect of the perfusion fluid blended with different compositions. Methods:30 rabbits were randomly divided into 5 groups which were control group(A), UW fluid with glyceryltrinitrate group(B), UW fluid with PGE group(C), UW fluid blended with auto-artery-blood (1:2) group(D) and UW fluid blended with auto-artery-blood (1:4) group(E). The lung and heart of rabbit were Dissociated as the isolated lung and heart model. the model were Perfused with low temperature perfusion fluid and conserved in the same temperature perfusion fluid. The lung specimens were obtained in order to analyze the change in morphology by using electron microscope at 2 and 4 hours after perfusion. the ratio of wet/dry weight(W/D), airway pressure(AWP) of lung model Detectd; were the blood which was pumped into the lung model through main pulmonary artery and then was elicited from atrium pulmonale by using a vacuum aspiration tube were detected after 4 hours of perfusion. Results:The difference of protection to the function of lung tissue gas exchange in E and D group was not remarkable, The protective effect to the function of the lung tissue gas exchange in E and D group surpasses the protective effect of other group significantly. There was no notable difference on the protective effect to the function of lung tissue gas exchange between B and C group. But the result of B and C group shows more superiority to group A. Conclusion:The experimental model was good in reliability and constancy. The group adding auto-artery-blood to the UW perfusion fluid can get longer time of preservation and better preservation effect of the function of lung tissue gas exchange than other group. Compared with the pure UW perfusion fluid group, the superiority of the preservation effect of perfusion fluid with glyceryltrinitrate and PGE to the function of lung is demonstrated again. But the preservation effect of the perfusion fluid with glyceryltrinitrate and PGE appeared inferior to that of the perfusion blended with auto-artery-blood.

Abstract:Objective:To prepare human herpesvirus 6 U94 protein and produce its antibodies. Methods:U94 gene of HHV6 was amplified by PCR and sequenced. The resulting DNA construct was cloned into a prokaryotic expression vector(PGEX-6P-1). The recombinant plasmid was transformed into Eserichia coli Rosetta. The accuracy of inserted gene and specificity of proteins were detected by two enzymes digestion, SDS-PAGE, and Western Blot. The fusion U94 protein purified by affinity chromatograph was used to vaccinate rabbit to produce antibodies. Results:The purity of U94 protein was above 90%. The titer of the antibodies was 1 ∶ 100 000. Conclusion:HHV6 U94 fusion protein with high purity was obtained and its corresponding polyclonal antibodies with high titer were produced.

Abstract:Objective:To investigate the effects of the aerobic sports and supplement L-carntine,the soybean petide to fat mouse among six weeks,analyze the influence about Lee-蒺s index and apoprotein. Methods:SD fat model mouse were taken as the reasearch object,they were taken a traning through 6 weeks and filled with the 10% nourishing everyday[600 mg/(kg·d) L-C and 15 mg/(kg·d)SP]. The control group were only fill with the distilled water 2 ml/d. The obesity rat’s body weight,the body long were observed,Lee-蒺s the index were calculated. Results:The obesity intervention group,nutrition intervention group and aerobic+nutrition intervention group-s Lee-s index obviously were lowerthan the obese comparison group,and have obvious difference(P < 0.05),but There was nodifference among the three experimental groups(P > 0.05); The nutrition intervention,the aerobic intervention group,the aerobic+ nutrition intervention are obviously reduced the apoB level(P < 0.05); The aerobic intervention and the nutrition intervene to the obesity rat-s apoA influence did not have the obvious change(P > 0.05),but the aerobic+ nutrition intervenes to the obesity rat-s apoA influence had obvious chang(P < 0.05). Conclusion:Through the nutrition feed 8 weeks,the obesity rat model were established sucessfully. The movement and the nutrition intervention can improve the lipin metabolism,remarkably reduce the obesity rat’s apoB density, and elevates the apoA density.

Abstract:Objective:To explore the effect of lamotrigine(LTG) and topiramate(TPM) on the behaviors and electroencephalogram (EEG) of epileptic rats induced by penicillin,and to explore the protection of LTG and TPM for the neurons of hippocampus. Methods:The seizures were observed in the group of epileptic rats induced by penicillin, the group of the rat given LTG, the group of the rat given TPM,the group of the rat given Sodium Valproate, and EEG was recorded in these groups. Immunohistochemical staining was used to observe the expression of Bcl-2 and Bax. Results:The epileptiform discharges decreased in the group of the rat given LTG and the group of the rat given Sodium Valproate, compared the group of the rat given LTG and TPM with the group of the rat given Sodium Valproate. We found that the latencies and durations of seizure between the group of the rat given LTG and the group of the rat given Sodium Valproate had significant difference(P < 0.01). Compared with the epileptic group induced by penicillin,Bcl-2 positive cells increased and Bax positive cells decreased in the group of the rat given LTG and the group of the rat given odium Valproate(P < 0.01). Compared with the group of the rat given Sodium Valproate,Bcl-2 positive cells in the group of the rats given LTG group and in the group of the rats given TPM were increased(P < 0.01). Conclusion:LTG and TPM was better than the Sodium Valproate in the anti-epileptic effects. LTG and TPM can protect hippocampal neuron in the epileptic rats induced by penicillin.

Abstract:Objective:To observe the change of the morphological structure in lung perfused with raffinose-low-potassium dextran solution(R-LPD-蒺S). Methods:The 20 pig donor lung perfused with low-potassium dextran solution(LPD-蒺S) and R-LPD-蒺S was observed by light microscope, electronic microscope and immunohistochemistry. Results:Morphologic changes of the structure in the tissue cell were gradually obvious along with time. After 24 h of preservation, the tissue of donor lung perfused with LPD-蒺S showed a decreased number of intact type Ⅱ pneumocytes and significant cellular necrosis. Interstitial and alveolar edema with interstitial macrophage infiltration was also observed. Alveolar capillaries were collapsed. In contrast, R-LPD’S lungs showed only mild alterations such as minor interstitial edematous expansion, fewer damaged cells, and minor capillary injury. Conclusion:R-LPD’S exerts a cytoprotective effect on preservation of donor lung during pulmonary grafts, and it should be superior to LPD-S for this purpose.

Abstract:Objective:To investigate the protective effects of prostaglandin E1(PGE1) on lung injury by regulating inflammatory system during cardiopulmonary bypass(CPB). Methods:From March to June in 2006,a total of 24 patients undergoing cardiac surgery were randomly divided into the control group(n = 12) and the experimental group(n = 12). Each patient in the experimental group received 15 ng(kg·min). min-1 of PGE1 from the induction of anaesthesia to the end of CPB. Blood PMN and Plt in left and right atrium were measured at 5 min before CPB and 15 min after heart resuscitation. The levels of sE-selectin and CRP in serum were measured at 5 min before CPB,30 min on CPB,15 min after heart resuscitation,1 h and 6 h after CPB. Oxygenation index(OI)and compliance of lung(CL) were also recorded in both groups. Results:①PMN and Plt count in the blood in left atrium were significantly lower than that in right atrium at 15 min after heart resuscitation in the control group(P < 0.01). ②During and after CPB,the level of sE-selectin increased in both groups, but it was significantly higher in the control group than that in the experimental group(P < 0.01). ③During and after CPB, the level of CRP in the experimental group was significantly lower than that in the control group(P < 0.01).④Compared with the control group, OI was significantly lower(P < 0.01) and CL was significantly higher in the experimental group(P < 0.01) at the end of CPB. Conclusion:PGE1 can effectively reduce the sequestration of neutrophil and platelet in the lungs,inhibit the activation of vascular endothelial cells and protect lungs during CPB.

Abstract:Objective:To explore the relationship between the circumstances of intra and extra cranial artery stenosis and the risk factors with ischemic cerebrovascular disease. Methods:347 patients were performed by using DSA, which were divided into young middle aged and old aged group by different age and the cerebrovascular stenosis(CVS) and non-stenosis(CVNS) group by with or without stenosis. Statistical analysis was used to evaluate the incidence, degree, distribution and risk factors of intra and extra cranial artery stenosis. Results:The prevalence of intra and extra cranial artery stenosis in ischemic cerebrovascular disease group is 152/347(43.8%). The frequency of stenosis of intracranial artery was 80/152(52.6%). The frequency of stenosis of extracranial artery was 21/152(13.8%). The frequency of stenosis of intracranial and extracranial artery was 51/152(33.6%). The degree of intra and extra cranial artery stenosis was positively correlated to the age(rs=0.156,P < 0.05). Hypertension(HBP),diabetes(DB),homocysteic(HCY),total cholesterol(TC),fibrin(FIB), in CVS group were significantly higher than that in CVNS group(P < 0.05). Conclusion: The incidence of stenosis of intracranial artery is higher than that of extracranial artery with cerebral artery stenosis of ischemic cerebrovascular disease. The distribution of cerebral artery stenosis correlated with age.The level of HBP, DB, HCY, TC and FIB was positively correlated to the rate of carotid artery stenosis.

Abstract:目的:了解2型糖尿病合并亚临床甲状腺功能减退(亚临床甲减)的认知功能特点-方法:通过认知功能问卷,对18例2型糖尿病合并亚临床甲减和102例甲状腺功能正常的2型糖尿病的认知功能特点及相关因素进行分析-结果:2型糖尿病合并亚临床甲减在MMSE总分-执行功能-语言复述-身体部位线索回忆-指图正确数-图片误认数均较甲功正常的2型糖尿病者差,两者差异具有显著性(P < 0.05);促甲状腺激素(TSH)水平与性别呈正相关(r = 0.310,P < 0.001)-结论:2型糖尿病合并亚临床甲减在执行功能-语言能力方面损害加重,而且有图形觉及记忆能力的进一步减退;女性2型糖尿病患者更易患亚临床甲减;亚临床甲减作为一种可逆性痴呆的常见原因,应引起临床足够重视-

Abstract:Objective:To explore the correlation of two functional nucleotid epolymorphisms in the cyclooxygenase-2(COX-2) gene with susceptibitity to gastric cancer in a Chinese population of Yixing city. Methods:Polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP) and the primer-introduced restriction analysis(PIRA-PCR) assay was used to defect genotype of the COX-2 -1195G/A and 8473T/C polymorphisms, respectively. In a case-control study, 254 gastric cancer cases and 304 cancer-free controls were included in a Chinese population of Yixing city. Results:There was no statistical difference in the genotype frequencies of these two polymorphisms between the cases and controls(P = 0.695 for -1195G/A and P = 0.762 for 8473T/C). Logistic regression analyses showed that both variant genotypes of COX-2 -1195 and COX-2 8473 polymorphisms were not significantly associated with susceptibility to gastric cancer(adjusted OR=1.09,95% CI=0.70-1.67 for SNP -1195 GA+AA and adjusted OR=1.18,95% CI=0.83-1.69 for SNP8473 TC+CC). Conclusion:These findings suggested that these two COX-2 polymorphisms may not be associated with genetic susceptibility to gastric cancer.

Abstract:Objective:To investigate the current status of acute poisoning in children in Nanjing city and analysis on its variation characterization and possible reason distribution. Methods:Local pediatric patients hospitalized for acute poisoning at Nanjing Children’s Hospital over a 3 year period(2004~2006) were evaluated retrospectively. Special attention was given to poisoning in relation to type, distribution in age and season, and general information as well. Results:The acute poisoning in children presented at special age. The frequency of children before school age was 93%, especially at the age of 2 years(32.5%). Drugs were the most common agent of poisoning(66.5%) and mostly were adult drugs such as drugs to depress blood-pressure(19.1%). The frequency of acute poisoning was higher in summer and autumn than that in spring and winter. The agents of poisoning between four seasons were different. Drug, pesticide, househood chemicals and food was the most agent in four seasons, respectively. Conclusion:The poisoning induced by drug misusage in children is the most frequent type of poisoning in Nanjing. Therefore, the standardized management and usage for drug should be strengthened in order to reduce the local occurrence of acute poisoning in children.

Abstract:Objective:To build a set of indexical system which can be applied to the three stages of selection,examination and acceptance in the evaluation of key clinical disciplines construction in small and medium hospitals. Methods:Based upon typical investigation, such methods as systematical analysis and brain storm were used to establish the first draft of evaluation indexical system. Then specialist consulting method was used to perfect it. Results:The evaluation system was composed of 5 first-degree criteria,22 second-degree quantified criteria with illustration of the evaluation system and weights. Conclusion:The evaluation system of key clinical disciplines construction in small and medium hospitals was constructed successfully.