Abstract:Objective:To study the growth inhibition effect and its molecular mechanism of SGC-7901 cells by the tumor suppressor gene hING4 in vitro. Methods:The hING4 gene was transfected into human gastric carcinoma cell line SGC-7901 with a replication-incompetent adenovirus vector. The mRNA transcriptions of hING4 in SGC-7901 cells was confirmed using RT-PCR. The anti-tumor efficacy of hING4 on SGC-7901 cells was evaluated by MTT,and cell apoptosis were detected by Hoechest 33258 staining and laser scanning confocal microscopy(LSCM). The cell cycle and distribution and apoptotic peak was evaluated by flow cytometry. Bcl-2 and Bax were explored by means of immunocytochemistry. Results:hING4 was proved successful transcription in SGC-7901 cells.hING4 can obviously inhibit proliferation and induce S phase reduction,G2/M phase arrest and cell apoptosis of SGC-7901. hING-4 can also down-regulate the expression of bcl-2 and up-regulate the expression of bax. Conclusion:hING4 can obviously inhibit proliferation and induces cell apoptosis of SGC-7901 cells. Down-regulating the expression of bcl-2 and up-regulating the expression of bax may be one of the molecular mechanisms involved in the anti-tumor effect.

Abstract:Objective:To prepare the hepatic-targeted Bovine serum albumin(BSA)liposome and study its physicochemical properties, to providing a new hepatic-targeted drug delivery system for protein-drug. Methods:Liposome was prepared by reverse phase evaporation method,and then the liposome was coated with O-carboxymethyl N-galactosylated chitosan(Gal-CMCS). The shape,particle size,Zeta potential,entrapment efficiency and stability were investigated. Results:Hepatic-targeted BSA liposome was round and uniform;its mean particle size was(284.1±5.41)nm;the entrapment efficiency was(34.21±1.73)%; Zeta potential was(+27.1±1.17) mV;and there was no obvious change in entrapment efficiency and particle size at 4℃ within 3 months. Conclusion:The hepatic-targeted BSA liposome was successfully prepared,with good stability and high entrapment efficiency,thus providing a new hepatic-targeted drug delivery system for protein-drug.

Abstract:Objective:To construct rat Pik3cb(phosphatidylinositol 3-kinase,catalytic,beta polypeptide) shRNA eukaryon plasmid express vector,and test its downregulating effect on Pik3cb mRNA expression for the application of RNA interference in restraining vein graft restenosis. Methods:Two shRNA of rat Pik3cb were design and synthesize according to the sequence of Pik3cb in the Genbank,annealed to form double strands and then cloned into pGenesil-1,and then the sequences were examed. After shRNAs were transfected into rat thoracic VSMC through METAFECTENETM,the transfection rate was identified by EGFP. pAkt-473 protein expression was detected by Western blot,and cell apoptosis were obtained through flow-cytometry. Results:The two sequences of synthesized shRNA named pU6-Pik3cb-shRNA-1 and pU6-Pik3cb-shRNA-2 were exactly the same with the design. The 48 h and 72 h transfection rate were 15.7% and 10.1%,respectively. The pAkt-473 protein expression significantly reduced,while the apoptosis cells were more significantly increased in the shRNA groups than the control groups(P < 0.05). The scramble shRNA did not interfere with the VSMC proliferation. Conclusion:Rat Pik3cb shRNA eukaryon express plasmid vectors were constructed successfully and effectively inhibited the proliferation of rat smooth mouse cells.

Abstract:Objective:To prepare clobetasol propionate liposome, investigate its stability and study its pharmacodynamics on guinea pig eczema model. Methods:Clobetasol propionate liposome was prepared with revolution thin film dispersion. The stability of indices,such as the content of the principal agent,grain size,encapsulation efficiency,was investigated. The pharmacodynamics of different preparation on guinea pig eczema model were studied by evaluating cellular infiltrates in dermis. Results:The content of the principal agent,grain size and encapsulation efficiency of liposome were(100.84 ± 2.0)%,(140.5 ± 56.8)nm,(90.1 ± 2.3 )%,respectively. No obvious changes were found in all the indices in the stability of liposome under three different storage condition.There was significant difference in cellular infiltration in dermis of CP liposome group and commercial CP cream group. Conclution:The preparation was proved to be of high encapsulation efficiency,good stability and better curative effect than cream.

Abstract:Objective:To investigate the changes of interstitial cells of Cajal(ICC) and hemeoxygenase-2(HO-2) in the colon of diabetic rats and the immunocolocalization of the ICC and HO-2 in the colon of rats,and discuss the possible mechanism of colon dysfunction associated with diabetes mellitus. Methods:The distribution and expressions of ICC and HO-2 in the proximal and distal colon tissues of rats were detected by immunohistochemistry. Immunolocalization of ICC and HO-2 was detected by confocal laser scanning microscopy. Results:ICC and HO-2 were mainly distributed in the myenteric plexus of the colon tissue,and the expressions of ICC and HO-2 in the proximal and distal colon tissues of diabetic rats were decreased compared with those in the control group(P < 0.05). Double immunofluorescence displayed that c-kit positive cells and HO-2 positive cells were expressed in the identical sites in the colon tissues of rats. Double expressions of ICC and HO-2 in the colon tissues of diabetic rats were decreased compared with those in the control group. Conclusion:Decreased expressions of ICC and HO-2 in the colon tissues might play an important role in the pathogenesis of colon dysfunction associated with diabetes mellitus; ICC may produce CO and intervene its role in the gastrointestinal motility.

Abstract:Objective:To detect the expression of PD-1 on allo-recipients’ PBMCs and T cells administrated with immunosuppressant and investigate the significance of positive cooperativity of PD-1 mAb combined with CsA on T cells. Methods:Double and single labeled FCM was used to detect the expression of PD-1 on PBMCs and T cells of patients. The mixed lymphocytes assay(MLA) was used to establish a model of the transplant rejection in vitro. MTT was used to detect the cooperativity of PD-1 mAb and CsA. Results:Compared with normal person,PD-1 was highly expressed on T cells in allo-recipients who administrated with immunosuppressant. In mixed lymphocytes assay,the expression of PD-1 seemed to be induced by CSA,and PD-1 mAb plus CsA displayed a positive cooperativity,which would owe to the low intensity of costimulatory signal. Conclusion:PD-1 might be used as a new target of immune interference of allograft survival.

Abstract:Objective:To investigate the AgNORs’ correlation between peripheral T lymphocyte nucleus and chromosome from the patients with tumor. Methods:Silver straining technique and KL image system were used to detect AgNORs of peripheral T lymphocyte nucleus and chromosome in 74 patients with esophageal carcinoma,68 patients with primary hepatic cancer,and 40 healthy control. The results were expressed in the ratio of silver staining nucleolus area to nucleus area(I.S%) and the chromosome AgNORs counts(AgNORs counts/cell). Results:①The I.S% of AgNORs and the chromosome AgNORs counts in patients with esophageal carcinoma(5.28 ± 0.86,5.64 ± 0.45) and patients with primary hepatic cancer(5.37 ± 0.94,5.75 ± 0.49) were lower than those in healthy control(7.56 ± 0.78,7.35 ± 0.55)(P < 0.01);②There was no statistical difference in I.S% of AgNORs and the chromosome AgNORs counts between patients with esophageal carcinoma and primary hepatic cancer(P > 0.05); ③In patients with esophageal carcinoma, primary hepatic cancer,and the healthy control,the I.S% of AgNORs was positively correlated with the chromosome AgNORs counts. Correlation coefficients were 0.85,0.80 and 0.87,respectively(P < 0.001). Conclusion:The I.S% of AgNORs and the chromosome AgNORs counts in peripheral T lymphocytes can be served as two useful immunologic diagnostic makers,but the former presents a more convenient and credible approach for clinical application than the latter.

Abstract:Objective:To investigate the association between Klotho gene polymorphisms and the risk of gastric cancer in Jiangsu population. Methods:In a case-control study of 151 patients and 145 controls,we genotyped G-395A in the promoter region and C1818T in exon 4 of Klotho gene by PCR-restriction fragment length polymorphism(PCR-RFLP) methods. The association between the genetic polymorphisms and the risk of the cancer was further examined by multivariate analysis. Results:The frequency of the A allele of G-395A polymorphism in patients(39.7%) was significantly higher than that in controls(28.2%,P = 0.038), with the adjusted OR=1.66 and 95℅CI 1.02~2.72,which was more pronounced among subgroups of youger males,smokers and drinkers. However,the frequency of C1818T did not show any significant difference between the patients and control subjects(P = 0.80). The two polymorphisms were in linkage disequilibrium(D = 0.449,r = 0.187) and in compliance with Hardy-Weniberg equilibrium in control. None of the haplotypes were significantly associated with the presence of the gastric cancer. Conclusion:The G-395A polymorphism in Klotho gene may positively contribute to the etiology of gastric cancer in Jiangsu population.

Abstract:Objective:To study the suitable concentration and the security of topical mitomycin C(MMC) in preventing postlaminectomy peridural adhesion by 7.0T Micro MRI. Methods:Laminectomies were performed at L1 in 30 rats. Cotton pads soaked either with 0.1 mg/ml(group B),0.3 mg/ml(group C),0.5 mg/ml(group D),0.7 mg/ml(group E) MMC,or saline(group A) were applied to the operative sites for five minutes. To determine the somatosensory evoked potential(SEP), the rats were killed 4 weeks after surgey. The specimens were prepared for determination of the degree of scar adhesion according to Rydell method and the area of the scar with MRI. Results:No obvious adhesion were found in group D and group E. Severe peridural adhesion were formed in the control group(group A). The area of the scar with Micro MRI in group D and E showed significant decrease. The SEP did not alter obviously. Conclusion:0.5 mg/ml MMC may be suitable concentration in preventing postlaminectomy peridural adhesion.

Abstract:Objective:To clone the cDNA of human Survivin,study its expression in E. Coli. and demonstrat its significance in the diagnosis of hepatoma. Methods:Using the isolated total RNA from human leukemic cell line HL60 as a template,the human Survivin encoding cDNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR) methods. The PCR product was cloned into prokaryotic expression vector pMD-18T and sequenced. The expression of recombinant plasmid pGEX-2TK-Survivin was induced in E.coli BL21 by IPTG, the expressed product was purified by GST protein purification system,then was cut GST from the expressed product useing Thrombin. After the identification of the Survivin protein by SDS-PAGE and Western blot,they were coated in ELISA plate to detect the expression in serum of hepatoma patient. Results:Human Survivin,an anti-apoptosis gene,express Surviving protein with a relative molecular mass of 16.2 ku was cloned and used preliminary in hepatoma patients. Conclusion:Human Survivin gene was cloned successfully;Survivin protein was successfully expressed,purified and preliminary used. It may provide preliminary labratory evidence based on Survivin for the diagnosis and therapy of carcinoma.

Abstract:Objective:To study the effects of Mycobacterium Phlei on cell apoptosis in SPCA/Ⅰ human lung cancer cells. Methods:The Cells were cultured in RPMI 1640 together with Mycobacterium Phlei at different concentrations for 36 hour. The changes of apoptosis of SPCA/Ⅰ human lung adenocarcinoma cells induced by Mycobacterium Phlei were detected by Annexin V: FITC Apoptosis detection kit,FACSAria flow cytometry and the inverted microscope. Results:The growth inhibitory rate had the positive correlations with concentrations of Mycobacterium Phlei on SPCA/Ⅰ human lung adenocarcinoma cells. Mycobacterium Phlei had significant effects on cell apoptosis in SPCA/Ⅰ human lung cancer cells. Typical apoptotic cells were seen whose bodies became smaller and rounder. Conclusion:Mycobacterium Phlei can induce apoptosis on SPCA/Ⅰ human lung adenocarcinoma cells and inhibit the tumor cell proliferation.

Abstract:Objective:To explore the synergistic anti-tumor effect of alcohol extract of verbena officinalis(EVO) and paclitaxel (PTX). Methods:S180 cells were cultured in vitro, and the growth inhibition effects of the two drugs used respectively and in combination were detected by MTT methods. S180 transplant model was established in male ICR mice, and the tumor growth inhibition effects of the two drugs were calculated by measuring tumor volume in every two days. Results:In vitro experiment showed that cell growth was significantly inhibited by PTX when EVO was added concomitantly at a dose of 20 mg/ml. Moreover,in vivo experiment demonstrated that EVO(1 g/kg) efficiently enhanced the anti-tumor effect of PTX at a dose of 10 mg/kg. Conclusion:A subclinical dose of EVO could greatly improve the anti-cancer effect of paclitaxel,which provide a new strategy to amplify the efficiency of chemotherapeutics without increasing its dose.

Abstract:Objective:To investigate the effects of a novel extracorporeal blood purification therapy-coupled plasma filtration adsorption(CPFA) on the function of monocytes in the multiple organ dysfunction syndromes(MODS) patients with severe infection. Methods:This study was a pospective,pilot,crossover clinical trial. A total of seven patients diagnosed MODS with severe infection were selected in this study. Patients were randomly allocated to both 10 hrs of coupled plasma filtration adsorption plus hemodialysis(CPFA,treatment A) and 10 hrs of high volume hemo- filtration(HVHF,treatment B) with 12 h interval in random order,that was to say each patient receiving A+B or B+A randomly. Plasma monocyte HLA-DR expression and leukocyte TNF--琢 production(both spontaneous and lipopolysaccharide stimulating) were tested before and after the treatment. TNF--琢 production from normal human monocytes(THP-1 cell) incubated with patients’ plasma obtained at 0,5,10 h of the treatment was also measured. Results:①The blood monocyte HLA-DR expression(%) increased after CPFA(84.32 ± 4.63 vs. 73.65 ± 11.52,P = 0.037) but no change after HVHV(P > 0.05).②Spontaneous and lipopolysaccharide inducing TNF--琢 production from patients’ whole blood increased over time with CPFA(P < 0.05). This increase was negative in HVHF group(P > 0.05). ③Patients’ serum suppressed production of TNF-a by cultured monocytes(THP-1). This suppression effect decreased over time with CPFA(P < 0.05). However,no significant difference was seen in HVHF group(P > 0.05). Conclusion:CPFA was superior to HVHF in improving the antigen presentation ability of monocyte and restoring leukocyte responsiveness to lipopolysaccharide. These findings suggest a potential role for CPFA in the treatment of MODS.

Abstract:Objective:To assess the value of tumor markers(TMs) protein chip diagnostic system C12 in the diagnosis of gastrointestinal cancer(GIC). Methods:The sera of 329 pathologically-confirmed GIC patients were detected by the C12 protein biochip system. The most relevant TMs and the contribution of various combinations of TMs to the improvement of diagnosis were determined. Results:The diagnostic rates of C12 biochip system in 329 GIC patients were 13.73%,33.33%,38.30%,58.03% for stagesⅠ,Ⅱ,Ⅲ and Ⅳ patients,respectively,and the overall diagnostic rate was 39.21%. There were statistically significant difference(P < 0.01) among all clinical stages(contingency table chi-square test), stage Ⅰversus stage Ⅲ,stage Ⅰversus stage Ⅳ,and stage Ⅱ versus stage Ⅳ. The other comparisons didn’t have statistical significance(P > 0.05). Among all the 12 TMs,the top three positive rates of 27.36%,19.76% and 19.45% were obtained from CEA,CA242 and CA19-9,respectively,which were correlated with the stage of GIC. Conclusion:The C12 biochip diagnostic system has some value in the diagnosis of advanced GIC,but its sensitivity for early stage is poor. The miniature and effective diagnostic system is needed in clinic urgently.

Abstract:Objective:Contrasting serum nitric oxide, superoxide dismutase catalase and between the acute biliary pancreatitis group and the normal group,to discuss the significance of each index. Methods:Forty clinical acute biliary pancreatitis patients were selected randomly and divide into two types(mild ABP and severe ABP), 50 policlinic medical examination people were selected as the comparison, serum NO,SOD and CAT levels were examined in every object respectively. Results:Serum NO and CAT levels in ABP patients were higher than those in normal people(P < 0.01) and elevated remarkably in SABP(P < 0.01). While serum SOD in ABP patients was lower than that in normal people(P < 0.01) and descended remarkably in SABP(P < 0.05). Conclusion:NO and CAT participated in ABP development, whose levels were parallel to ABP status. SOD descended according to ABP status,providing that extrinsic SOD might alleviate the severity of ABP.

Abstract:Objective:To explore the Predictor of cerebrospinal fluid monoamine metabolites in depressed inpatients with suicidal Bebaviors. Methods:Twenty-four patients with major depression and 25 controls were enrolled in the study. The CSF concentrations of 5-hydroxyindoleacetic acid(5-HIAA),norepinephrine(NE) metabolites 3-methoxy-4-hydroxyphenylglycol(MHPG) and dopamine(DA) metabolites homovanillic acid(HVA) were measured by high-pressure liquid chromatography. Twenty-four depressed patients were followed up in 5 years. The relationship between CSF monoamine metabolites and present suicidal behaviours(suicidal group and non-suicidal group) or suicidal behaviours followed up(suicidal group and non-suicidal group). Results:CSF 5-HIAA levels in suicidal group this time were significantly lower than those in control group. CSF MHPG levels in non-suicidal group this time were significantly lower than those in control group. CSF HVA levels in suicidal group this time were significantly lower than those in control group. CSF HVA levels in suicidal group this time were significantly lower than those in non-suicidal group this time. CSF 5-HIAA levels in suicidal group followed up were significantly lower than those in control group. Conclusion:It is suggested that 5-HIAA is associated with suicidal bebaviors. In depressed patients,5-HIAA perhaps can predict the suicidal behaviors.

Abstract:Objective:To observe the effect of induction of force-injection with midazolam on haemodynamics and serum cortisol response to tracheal intubation in elderly patients. Methods:Sixty-four ASA Ⅰor Ⅱ patients aged over 65-year-old weighing 61~74 kg undergoing selective operation under general anesthesia were randomized into 4 groups(n = 16 each) according to midazolam doses-0.01(Ⅰ),0.02(Ⅱ),0.03(Ⅲ),0(Ⅳ) mg/kg before induction of anesthesia. 5 minutes later,anesthesia was induced with midazolam(Ⅰ0.04 mg/kg,Ⅱ 0.03 mg/kg,Ⅲ 0.02 mg/kg and Ⅳ 0.05 mg/kg),fentanyl 2 -滋g/kg,etomidate 0.3 mg/kg and rocuronium 1 mg/kg. Tracheal intubation was performed at 1.5 min after rocuronium injection. BP(SBP and MAP), HR, SpO2 and RR were continuously monitored and recorded in the resting state entering room(T1,baseline),5 min after midazolam injection(T2),immediately before intubation(T3),immediately and 1,2,3,4,5 min after intubation(T4,5,6,7,8,9). Blood samples were taken from artery at T1 and T9 for determination of plasma serum cortisol concentration. Results:SBP,MAP and HR decreased significantly at T2 as compared to the baseline values at T1 in the experimental groups. Compared with those in the experimental groups,the SBP,MAP and HR and variation of SBP and HR at T4 in group Ⅳ became higher. Compared with group Ⅲ,the variation of SBP and HR decreased significantly in groupⅠ. The serum cortisol was significantly smaller in group Ⅰ,Ⅱ and Ⅲ at T9 than at baseline. But it was contrary in group Ⅳ. Conclusion:Induction of force-injection with midazolam can decrease the stress response to tracheal intubation in elderly patients effectively.

Abstract:Objective:To investigate the changs of cell adhesion molecules and observe the influence with ulinastatin during the infant cardiopulmonary bypass. Methods:Sixty infants with congenital heart disease were randomly divided into two groups:group U(received ulinastatin 20 000 U/kg,dissolved in 20 ml normal saline),group C(received 20 ml normal saline). Blood samples were collected in different moments. The proteinum expressions of ICAM-1 and CD11b/CD18 were examined,and the variations were observed. The blood gas value and calculated respiratory index(RI) were also detected. Results:The expressions of ICAM-1 and CD11b/CD18 increased significantly after CPB in two groups with RI rising,and increased more obviously in group C than those in group U(P < 0.05). Conclusion:The expressions of ICAM-1 and CD11b/CD18 increased,and RI rose during the CPB in infant. Ulinastatin may relieve the process.

Abstract:Objective:To Observe the related factors and the preventive measures of pancreatic infection in severe acute pancreatitis. Methods:138 cases were divided randomly into two groups group (infection group and non-infection group) according to whether secondary infection in two weeks after onset of pancreatitis, and analyzed predisposing factors. Results:There was significant difference between infection group and non-infection group in the hypoxemia, necrosis of pancreas, gastrointestinal tract dysfunction, operation, biliary pancreatitis(P < 0.05). Conclusion:Gastrointestinal tract dysfunction≥5 day, hypoxemia, necrosis of pancreas, biliary pancreatitis are principal predisposing factors of pancreatic infection in earlier period of SAP.

Abstract:Objective:To establish the vascular blocking model in the left lung of rabbit and investigate the lung ischemia-reperfusion injury in pulmonary surgery. Methods:96 New Zealand rabbits were randomly divided into 3 groups:groupⅠ, control group; groupⅡ, blocking pulmonary artery; groupⅢ, blocking pulmonary artery and vein. After the time of opening chest(groupⅠ),1hour’s ischemia and 1,2,4,6 hours, reperfusion ,the changes of partial pressure of oxygen in arterial blood(PaO2) and the content of MDA in left lung tissue were observed. Then the water content of left lung and pathological study were recorded. Results:Homodynamic parameters were stable in all 3 groups. There were significant differences in PaO2, MDA and wet/dry ratio of the lung tissue between groupⅠand the other two groups(P < 0.05 and P < 0.01),and there was no significant difference between group Ⅱ and group Ⅲ(P > 0.05). Pathological study revealed that similar injury changes happened between group Ⅱ and groupⅢ, and the obvious injury happened at the time of 4 hours, reperfusion. At the time of 6 hours, reperfusion pathological changes in both group Ⅱ and group Ⅲ began to recover. Conclusion:This provides a simple, reliable and inexpensive model for investigating the lung ischemia-reperfusion injury in pulmonary surgery.

Abstract:Objective:To investigate the feasibility and efficacy of catheter ablation guided by 3-dimensional mapping system in patients with focal atrial tachycardia. Methods:From August 2004 to October 2007,catheter ablation guided by CARTO system was performed in 42 consecutive patients with significantly symptomatic,drug refractory atrial tachycardia. The procedural end-point is the atrial tachycardia teminated and could not be induced by drug and/or programmed electrophysiological stimulation. Results:A total of 41 atrial tachycardia foci were successfully ablated in 42 patients(97.6%). The sites of origin included from the pulmonary vein(21/42,50%),near the mitral annulus(5/42,11.9%),the left auricle area(2/42,4.8%),the right auricle area(2/42,4.8%),left atrium roof(1/42,2.4%),left atrium posterior lateral wall(2/42,4.8%),paraHis bundle(1/42,2.4%),right atrium superior anterior wall(2/42,4.8%),right atrium inferior wall(1/42,2.1%),coronary sinus ostium(1/42,2.1%) and right atrium posterior septum(2/42,4.8%). There were no other clinical complications except for two cases of Femoral vein hematoma. Atrial tachyarrhythmia free rate was 95.2% (40/42) during the 4~18(9.95 ± 3.9) months in follow-up. Conclusion:Mapping and radiofrequency catheter ablation guided by 3-D mapping system was effective and safe in eliminating focal atrial tachycardia.

Abstract:Objective:To evaluate the clinical value of dynamic enhanced multi-slice spiral computed tomography(MSCT) in solitary pulmonary nodules(SPN). Methods:40 SPN(25 malignant nodules, 8 inflammatory nodules, and 7 benign nodules) were scanned firstly to determine the nodules range and then scanned through the center of them after contrast media were performed with 5 ml/s or 3 ml/s. The pre-and post-contrast density, peak height after enhanced(PH) and peak time(PT) of SPN were recorded. Results: Moderate enhancement appeared mostly in lung carcinoma and the increased CT density varied from 20 to 60 Hu in 20/25 cases(80%). Peak height of lung cancer was(31.31 ± 10.62)Hu, peak time was 45 s, and the time-density curve represent slowly uptrend. Marked enhancement appeared mostly in inflammatory nodules,and the increased CT density was morn than 40 Hu. Peak height of inflammatory nodules was(49.25 ± 12.44)Hu, and peak time was 80 and 140 s. The curve’s trend of enhancement was wavy. Conclusion:Dynamic contrast enhanced CT can reflect the hemodynamic characteristic of SPN and be used non-invasively in the evaluation of solitary pulmonary nodules.

Abstract:Objective:To analyze the CT indicating features of hepatic high perfusion disorders at the hepatic arterial phase during the MSCT 3-phases liver scanning and the relationship with fundamental diseases, and discuss the mechanism. Methods:One thousand one hundred and forty-eight cases were conducted with CT plain scanning at the hepatobiliary region. After the contrast injection at the elbow vein,the 3-phases scanning,i.e. arterial,portal venous and balance phase,was conducted at the time of 25 s,60 s and 180 s, respectively. Results:One hundred and fifty cases of hepatic high perfusion disorders were found at the arterial phase and the abnormal shape of wedge,triangle,block or irregular of uniform high density. Most of high perfusion disorders appeared at the edge of the liver. Part of them was still shown slightly high density at the portal venous phase, and they appeared equal-density at the balance phase. Conclusion:MSCT 3-phases enhancement scanning is the best method for studying the hepatic high perfusion disorders at present. It is capable of avoiding the false positive and false negative diagnosis of liver tumors and other diseases and exaggerating the sizes of liver lesions. It is helpful to determine the condition and prognosis of some diseases.

Abstract:Objective:To know about the knowledge of migrant workers in Jiangsu area about AIDS,including the basic knowledge,means of transmission,and its clinical symptoms, and analyze the influencing factors on their knowledge. Methods:1668 migrant workers in the factories and construction sites were investigated in Nanjing and Kunshan of Jiangsu Province, and the univariate analysis and multivariate logistic regression analysis were used to explore the influencing factors on their situation of knowledge. Results:In general,the migrant workers in Jiangsu area lack knowledge about AIDS. Most of them know little about the means of non-aids transmission and the clinical symptoms of AIDS in particular,and the percentages of workers knowing about the two aspects are 26.62% and 27.27%, respectively. The percentage of women having knowledge about AIDS is lower than that of men,and workers with higher educational level know more about AIDS. Conclusion:AIDS health education should be carried out urgently among migrant workers to improve their knowledge about it.

Abstract:Objective:To collect the data of drug resistance to evaluate the prevalence trend of 10 anti-tuberculosis drugs commonly.Methods:The study objectives were from 2001 to 2006. Susceptibility tests(absolute concentration methods for INH,SM,RFP,EMB,TH1321,PAS,AN,KM,OFLX and CPM) were done in all specimens. Results:1800 tuberculosis cases involved in the study. The culture positive rate was 70%. The rate of the tuberculosis anti-multidrugs was 54.9%. Conclusion:The rate of the tuberculosis mutidrug resistant is very different. Some of drug’s resistance trend were rising yearly. The anti-tuberculosis drug resistant surveillance were established. The reliable data on the status of anti-tuberculosis drug resistance results provided the evidences for improvement and assessment on TB control and clinical drug use.

Abstract:Objective:To investigate the distribution of clinical fungus infection and the analysis of antibiotic resistance. Methods:Fungi were identified with bioMérieux Inc’s ATB Expression microbe identification system,ID32C strip and CHROMagar chromogenic substrate. Drug susceptibility test was executed with ATB Fungus drug susceptibility plate. Results:Candida albicans,candida tropicalis and candida glabrata are the main microorganism at clinical fungal infections; and they are distributed mainly among respiratory ward,cardiovascular ward,intensive care unit and infective ward; fungi show drug susceptibility to amphotericin B and 5-flucytosin and drug resistance against fluconazole to some extent. Conclusion:Clinic suspect samples should be tested in time,and antifungal drugs should be applied on the basis of drug susceptibility test to minimize the incidents of deep fungal infections.