Abstract:Objective:To investigate the effect on post-infarct heart function and apoptosis of cardiocyte with Ad5-HGF(adenovirus-mediated human hepatocyte growth factor) transference in swine models. Methods:Total 12 young swine were randomly divided into 2 groups, which were control group(null-Ad5,1 ml) and Ad5-HGF group(5 × 109 Pfu/ml,1ml). In four weeks after left anterior descending(LAD) coronary artery ligation,Ad5-HGF(5 × 109 Pfu/ml,1 ml)was transferred in the therapeutic group,and null-Ad5(1 ml) was transferred in the control group via right coronary artery. At 4th and 7th week after LAD ligation, gate cardiac perfusion imaging was performed to evaluate cardiac perfusion and LVEF. Swine were sacrificed in the 7th week,the apoptotic index of cardiocyte was observed by TUNEL, and the expression of Bcl-2 and Bax protein were evaluated by immunohistochemistry. Results:①In four weeks after LAD ligation,no difference was found between the control group and Ad5-HGF group in the cardiac perfusion and LVEF(P > 0.05). At three weeks after gene transfer,heart function was significantly improved in the Ad5-HGF group(P < 0.01), while unchanged in the control group(P > 0.05). The improvement of heart function was greater in the Ad5-HGF group than that in the control group(P < 0.01). ②TUNEL assay showed that there were lots of apoptotic cardioctyes in the border zone in two groups,but the Ad5-HGF group had a more obvious reduction in the apoptotic index, compared with the control group(P < 0.01). ③The expression of Bcl-2 protein was increased and the expression of Bax protein was inhibited in the Ad5-HGF group, compared with that of the control group(P < 0.01). Conclusion:Ad5-HGF transferred via noninfarct-related coronary artery could reduce apoptosis of cardiocyte and ameliorate post-infarct heart function. The up-regulation of Bcl-2 and down-regulation of Bax may play an important role in the anti-apoptotic effect of Ad5-HGF.

Abstract:Objective:To investigate the effect of resveratrol on the density of L-type calcium current(ICa-L) in cultured guinea pig ventricular myocytes. Methods:Single cultured ventricular cells were obtained from healthy adult male guinea pig heart using enzymatic dissociation methods. The whole-cell patch clamp was used to record the ICa-L after administration of resveratrol at 24 h and 72 h with different final concentration of 0,10,30,100 μmol/L. Results:①30 μmol/L and 100 μmol/L RES decreased the density of ICa-L by 13% and 27%,respectively,at 72 h. There is a significant difference between control group and RES 100 μmol/L group(P < 0.05);②RES 10 μmol/L,30 μmol/L and 100 μmol/L have a tendency of reducing the density of ICa-L in a time-dependent manner. 100 μmol/L RES reduced ICa-L more significantly at 72 h compared with that did at 24 h(P < 0.05). Conclusion:Resveratrol have a tendency of inhibiting the density of L-type calcium current in cultured guinea pig ventricular myocytes at a concentration-and time-dependent manner.

Abstract:Objective:To investigate the effect of BVT.2733 on insulin resistance(IR) and inflammation in diet-induced obesity(DIO)mice. Methods:Male mice were divided into normal mice and model mice after fed with normal diet and high-fat diet for 20 weeks,respectively. The model mice were subdivided into BVT.2733 mice administrated with BVT.2733 and obesity mice with placebo for 2 weeks. Indexes related with metabolism and inflammation were measured. Results:Compared with normal mice,adipocytes were significantly enlarged in obesity mice,and body weight also increased. Similarly,blood glucose,serum insulin and TNF-α,the expression of TLR4 mRNA in adipose tissue were all increased. After administration of BVT.2733,adipocytes were diminished; body weight,blood glucose,serum insulin,and the expression of TLR4 mRNA in adipose tissue were all decreased. But there was no significant change in serum TNF-α. Conclusion:BVT.2733 can improve IR and decrease the expression of TLR4 mRNA in adipose tissue,but have little effect on serum TNF-α in DIO mice.

Abstract:Objective:To establish the nonalcoholic fatty liver model of rat induced by high fat diet. Methods:Twenty Sprague-Dawley(SD)rats were divided randomly into two groups:normal control group fed with common diet and model group fed with high fat diet for 4 weeks. The general conditions and weight changes were observed for 4 weeks. All rat were sacrificed at the end of 4 th week of experiment. Blood samples were collected for measure the alanine aminotransferase(ALT),albumin(ALB),triglyceride(TG). The liver specimens were stained with HE for histology. Liver index was counted. Results:The liver index and triglyceride(TG) of model group were significantly higher than those of control group. The alanine aminotransferase(ALT) and albumin(ALB) between two groups didn’t have significant difference. The histological observation showed mild to moderate hepatic steatosis. Conclusion:The nonalcoholic fatty liver model of rat was successfully established.

Abstract:Objective:The casting sample of rat liver was fabricated, and the data of every section was harvested. The 3D-reconstruction of digital model of portal veins was reconstructed with computer and software. Methods:The propylene pigment preparation was injected into the rat portal vein(20 cases). The sample was embedded and cut into frozen sections. The data of every section of rat liver was collected by digital camera. Image segmentation and 3D-reconstruction were applied in photos. Results:Eighteen cases of the casting sample of rat liver were made successfully by this method. The propylene pigment preparation can enter small blood vessel whose diameter was 50~100 -滋m. Every case had about 200~300 photographs of the sections by an interval of 50 -滋m. They can be showed by computer vision based three-dimensional reconstruction. Conclusion:The method described above is feasible to get experimental data for 3D-reconstruction of digital model of rat liver and portal veins.

Abstract:Objective:To investigate the effects of pamidronate on the osteoclasts activated by the polyethylene particles and explore the preventing effect of pamidronate on the aseptic loosening of prosthesis. Methods:The osteoclasts were isolated from the long bones of neonatal New Zealand’s rabbits and cocultured with bone slices to observe the effects of pamidronate and polyethylene particles on the osteoclasts’ resorption activity. First, the influence of polyethylene particles of different dosage on the osteoclasts’ resorption activity were observed;Second, the influence of polyethylene particles associated with pamidronate on the osteoclasts’ resorption activity were observed. Results:We found that three kinds of polyethylene particles(103/ml,106/ml and 109/ml)all can stimulate the osteoclasts’ bone resorption, and pamidronate of 10 μg/ml can inhibit the stimulation of polyethylene particles on the osteoclasts. Conclusion:Because pamidronate can inhibit the activation of osteoclasts induced by the polyethylene particles,it can be probably applied to prevent the aseptic loosening of prosthesis.

Abstract:Objective:To determine the role of 1α,25(OH)2D in dentin formation and predentin mineralization of mice. Methods:The differences of the dentin formation and predentin mineralization between the wild-type and 1--琢-hydroxylase gene knockout mice at 6 weeks old were assessed by histology,immunohistochemistry and RT-PCR. Results:The dental volume,the deposition of type I collagen and osteocalcin in tooth matrix, and the expression of osteocalcin in odontoblasts were reduced significantly. But the predentin volume was increased in 1--琢-hydroxylase gene knockout mice compared to that in the wild-type littermates. Conclusion:These studies suggest that 1α,25(OH)2D is essential for the dentin formation and predentin mineralization in mice.

Abstract:Objective:To assess the efficacy, safety and mechanism of self-expanding sirolimus-eluting stent implanted in porcine carotid artery for preventing from early restenosis after angioplasty. Methods:After angioplasty on the dual common carotid arteries by ballon overstretch,6 sirolimus-eluting stents and 6 bare metal stents were implanted in 12 injured carotid arteries of 6 chinese minipigs at random, respectively. Four weeks later,after angiography 6 chinese minipigs were executed. Lumen area,neointimal thickness and area of injured arteries segment were measured by computer image analysis technique. The arteries segment were removed for measurement of the apoptotic index by TUNEL assay and the expression of proliferating cell nuclear antigen(PCNA),Bcl-2 and Bax proteins by immunohistochemistry. Results:Compared with the bare metal stent group,the lumen area of sirolimus group was significantly greater while the neointimal thickness and area was significantly smaller(P < 0.05). PCNA and Bcl-2 expression was significantly decreased in sirolimus-eluting stent group(P < 0.05), while apoptotic index and Bax expression was significantly increased(P < 0.05). Conclusion:Sirolimus-eluting stent can effectively inhibit neointimal formation to prevent early restenosis in porcine common carotid artery 4 weeks after implantation,with the possible mechanism of upregulating the expression of Bax,downregulating the expression of Bcl-2 and inhibiting vascular smooth muscle cell proliferation.

Abstract:Objective:To investgate the inhibiting effect of Weining granule on the growth and metastasis of orthotopic human gastric carcinoma. Methods:To establish the model of orthotopic human gastric carcinoma,60 rats with orthotopic human gastric carcinoma were randomly divided into the control group,CTX group,Weining granule group,CTX with Weining granule group,12 in each group. The inhibiting rate of carcinoma’s volume and weight,the change of shape on carcinoma cells,the expression of apoptotic index(AI),p53,Bcl-2,bax,CD44v6 and PCNA were all measured. Results:The inhibiting rate of carcinoma’s volume and weight in all CTX group,Weining granule group,CTX with Weining granule group were different from that of the control group(P < 0.05); the inhibiting rate of carcinoma’s volume and weight in CTX with Weining granule group were different from that of the CTX group(P < 0.05); the rate of hepatic metastasis in CTX group and Weining granule group were less than that of the control group(P < 0.05,P < 0.01);AI,p53,bcl-2,bax,CD44v6 and PCNA expression in Weining granule group were significantly different from that in the control group(P < 0.05);p53,CD44v6 and PCNA expression in CTX with Weining granule group were less than that in the CTX group(P < 0.05). Conclusion:Weining granule can inhibit the growth and metastasis of orthotopic human gastric carcinoma.

Abstract:Objective:To investigate the relationship between the coronary heart disease and the microsomal triglyceride transfer protein(MTP)-493G/T gene polymorphism. Methods:There was a case-control study,which enrolled 335 non-diabetic patients with coronary heart disease(CHD) and 206 control subjects without CHD. The genotype and allele frequencies of MTP-493G/T gene polymorphism were detected by polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP). Results:The highest frequency of MTP-493G/T genetic variation was GG genotype; the second was GT genotype; and the lowest was TT genotype in Han population of Jiangsu Province,China. Both the TT genotype frequency(6%) and the “T” allele frequency(19%) in the patients with CHD were higher than those in the control(3% and 12%), respectively(P < 0.05). But there wasn’t a significant correlation between MTP-493G/T genotypes and the number of significantly diseased vessels. The TT genotype had the higher TG and LDL-C level(P < 0.05) than GG genotype. In multiple Logistic regression analysis,the TT genotype odds ratio for coronary heart disease was 2.14(95%CI 1.22~6.56,P = 0.014). Conclusion:The TT genotype of MTP-493G/T genetic variation might increase the risk of coronary heart disease, independent of lipid and lipoprotein factors.

Abstract:Objective:To investigate the association of loci 1587 polymorphisms of ATP binding cassette transporter 1 gene with coronary heart disease(CHD) and lipoprotein levels. Methods:Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism in 260 patients with CHD and 248 control subjects. Results:Three genotypes were shown in the present study. RR, RK and KK genotypes accounted for 76.97%,18.11% and 4.92%, respectively. Neither the allele frequencies nor genotypes showed significant difference between patients with CHD and control subjects(P > 0.05). The HDL-C levels was lower in K allele carriers than in non-K allele carriers. Conclusion:The genotypes of ABCA1 R1587K polymorphism were associated with the plasma levels of HDL-C. The HDL-C level of K allele carriers was remarkably lower than that in non-K allele carriers. However,there was no significant association of ABCA1 R1587K polymorphism with CHD.

Abstract:Objective:To investigate the correlation between the stenosis degree of coronary and the left ventricular cardiac dysfunction. Methods:The coronary stenosis degree were analyzed retrospectively on 117 patients(male 91,female 26) by angiography. The parameters of blood pressure,serum lipid and echocardiography were observed. According to Gensini’s score, the correlation between the stenosis degree of coronary and cardiac dysfunction were analyzed. Results:When Gensini’s score level was examined as a categorical variable ordering by quartile values. There were significant differences in diastolic blood pressure(DBP)(P = 0.019),left atrial dimension(LAD)(P = 0.003),left ventricular end diastolic dimension(LVDd)(P = 0.000),left ventricular end systolic dimension(LVDs)(P = 0.000), fraction shortening(FS)(P = 0.001), left ventricular ejection fraction(LVEF)(P = 0.000) and HDL-C(P = 0.016) among the groups with different Gensini’s scores. Compared Gensini’s scores with Spearman, Gensini’s score was positively correlated with LAD(R = 0.341,P = 0.000),LVDd(R = 0.373,P = 0.000) and LVDs(R = 0.399,P = 0.000),respectively. Gensini’s score was negatively correlated with FS(R = -0.298,P = 0.001),LVEF(R = -0.320,P = 0.000) and HDL-C(R = -0.276,P = 0.003). By the multiple linear regression between the Gensini’s score and paramenters,LVDs and DBP were independently associated with the scores of the stenosis degree of coronary. LVDs and DBP were independently associated with Gensini’s score. Conclusion:LVEF and FS were negatively correlated with the stenosis degree of coronary and independent from other tradiovascular risk factors.

Abstract:Objective:To investigate the types and prognosis of ectopic arrhythmia(EA) after transcatheter closure of perimembranous ventricular septal defects(PMVSD)and the risk factors of EA. Methods:228 patients with PMVSD were accepted transcatheter closure successfully. Electrocardiogram(ECG) and Holter ambulatory electrocardiographic monitoring were underwent in all patients both pre-and post-procedure,as well as 1,3,6 months and every year after the procedure. Results:Sixty-one case-times of new EA(19.7%) were detected in 45 patients which included 25 case-times of accelerated idioventricular rhythm,14 case-times of accelerated idiojunctional rhythm,14 case-times of ventricular premature beat and 8 case-times of junctional escape rhythm. All the patients with EA had not been given special treatment,EA in 43(95.6%) cases sustained(4.16 ± 2.71) days,2 cases recovered sinus rhythm in the first month of follow-up,and new EA was not found during the follow-up. It was found that the risk factors of EA after closure were related to the distance from the lower margin of the defects to septal leaflet of the tricuspid valve(DDSTV) and the distance from upper margin of the defects to the ring of the right aortic valve(DDRAV). DDSTV ≤ 2.45 mm or DDRAV ≥ 3.05 mm has good predictive value of the incidence of EA. Conclusion:EA is a common complication after transcatheter closure of PMVSD,but with better prognosis in our study. We should closely monitor ECG changes and treat the patients with the risk factors after interventional treatment in time.

Abstract:Objective:To investigate the association of the diagnostic components of metabolic syndrome(MS) with the cardiac function in Chinese. Methods:In 843 subjects recruited in Zhejiang province,we measured each participant’s body height,body weight,waist and hip circumferences,and blood pressure. The observers were administered a standardized questionnaire to collect information on smoking habits,alcohol consumption and use of antihypertensive drugs. Echocardiography was performed on each subject according to the recommendations of the American Society of Echocardiography for the measurements of LVEF,E/A ratio,DTE,and Tei index. Venous blood was sampled for biochemical measurements. Results:843 subjects included 288(34.2%) women,and 191(22.7%) hypertensive patients,of whom 82(42.9%) took antihypertensive drugs. Compared to subjects in normal group,MS patients had significiantly lower LVEF(P = 0.049) and E/A ratio(P < 0.001),and higher DTE(P < 0.001) and Tei index(P < 0.001). Moreover,LVEF and E/A ratio decreased,and DTE and Tei index increased, with the number of MS components. Conclusion:The number of MS-diagnosis components was associated with the impairment of cardiac function.

Abstract:Objective:To observe the ultrastructure feature of thyroid papillary carcinoma with neuroendocrine differentiation. Methods:The paraffin-embedded tissues were used,including 103 cases with thyroid papillary carcinoma,all of which were marked with CgA,Syn and NSE by immunohistochemistry, respectively. Those 66 cases of papillary carcinoma with positive expression of CgA,Syn,NSE were examined by electron microscope. The clinical parameters included cellular differentiation degree, nucleus feature,age,psammoma,tumor diameter,metastasis of lymph nodes,tumor capsule and surrounding-tissue invasion. STATA8.0 medical statistic software was adopted in statistical analysis. Results:18.2% cases with thyroid papillary carcinoma found neuroendocrine particles under the electron microscope. Cellular differentiation degree of papillary carcinoma and the tumor size had relevance to the neuroendocrine differentiation(P < 0.05),but the other parameters had no relevance to neuroendocrine differentiation. Conclusion:The phenomenon of neuroendocrine differentiation exists in partial cases with thyroid papillary carcinoma, and the tissues of thyroid papillary carcinoma with neuroendocrine differentiation have poor differentiation and large tumor size.

Abstract:Objective:To investigate the gene expression of sodium/iodide symporter(NIS) protein in papillary thyroid carcinoma(PTC) and the relationship between NIS and PTC. Methods:Thyroid tissue was obtained from 22 patients with PTC,12 samples of normal thyroid tissue(NT),7 samples of lymph node metastasis tissue(LN),and 12 samples of nodular goiter tissue(NG),respectively. The expression of NIS was evaluated by the Western blot technique. Results:NIS positive ratio in PTC and LN tissue was 77.3% and 85.7% respectively,higher than that in NT and NG. Difference between experimental groups was significant(P < 0.05). 16 samples of 17 NIS positive patients with PTC were mainly expressed in the cytoplasm,and only 2 samples were detected in the membrane. The difference was also significant(P < 0.05). Conclusion:The NIS protein in PTC was normally expressed,even over-expressed,and was mostly expressed in the cytoplasm rather than in the functional membrane,which related to the alteration of translation and modification caused by malignant tumor.

Abstract:Objective:To investigate the expression and clinical significance of hypoxia-inducible factor 1α(HIF-1α) in hypoxic human lung cancer cell lines and human lung carcinoma tissues. Methods:Transwell assay was used to test the invasive capacity of hypoxic lung cancer cells. The expression of HIF-1α mRNA and protein in hypoxic lung cancer cells in vitro was determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. The expression of HIF-1α was detected using immunohistochemistry and reverse transcription-polymerase chain reaction analyses(RT-PCR) in 60 specimens of lung carcinoma and 20 specimens of corresponding adjacent tissues. Results:The effect of hypoxia on the invasion of lung cancer cells increased. After hypoxia exposure,the HIF-1α mRNA and protein expression in lung cancer cells increased time-dependently. Compared with those in the control group,the mRNA and protein levels of HIF-1α in the hypoxic groups had more significant difference(P < 0.05 or P < 0.01). In lung carcinoma tissues,the positive expression rates of HIF-1α mRNA and proteins were 90.0% and 75.0% ,while 30.0% and 20.0% in normal lung tissues respectively,showing the expression of HIF-1α in cancer tissues were significantly different from that in normal tissues(P < 0.01). Comparing with the mRNA and protein levels of HIF-1α in lung cancer tissues of ⅠandⅡstage,there was marked increase in Ⅲ and Ⅳstage. The expression of HIF-1α in group of lymph node metastasis was higher than that in group without lymph node metastasis(P < 0.05). Conclusion:CoCl2 can up-regulate the expression of HIF-1α in lung cancer cell lines by inducing hypoxia. These hypoxia-mediated effects have the potential impact on lung cancer cells to promote its hypoxic tolerance. HIF-1-琢 overexpression plays important roles in invasion and metastasis of lung carcinoma. HIF-1-琢 may be a useful marker for evaluating the biological behaviors of lung carcinoma.

Abstract:Objective:To investigate the expression of transforming growth factor beta 1(TGF-β1) and matrix metalloproteinases 2(MMP2) in human renal clear cell carcinoma(RCCC) and their clinical significance. Methods:The protein expression of TGF-β1 and MMP2 in 80 cases of RCCC and 30 cases of tissues adjacent to cancer were determined with immunohistochemical SP method. The mRNA expression of TGF-β1 and MMP2 gene in 43 cases of RCCC and 43 cases of tissues adjacent to cancer were determined with reverse transcription-polymerase chain reaction(RT-PCR). Results:In RCCC group TGF-β1 and MMP2 expression intensity was obviously higher than that in tissues adjacent to cancer. The expression of TGF-β1 and MMP2 were correlated with the pathologic grade and clinical stage of RCCC. There was a statistically significant correlation between expressions of TGF-β1 and MMP2. The high expressions of TGF-β1 and MMP2 significantly correlated with the poor prognosis of patients with RCCC by univariate survival analysis(P < 0.05,respectively). Conclusion:Up-regulation of TGF-β1 and MMP2 expression may be involved in the progression of RCCC. The correlation was found between TGF-β1 and MMP2. This indicates that TGF-β1 and MMP2 can be used as a useful associated-molecular marker for prognosis of RCCC.

Abstract:Objective:To investigate the role of tazarotene induced gene-2(TIG2) in the pathogenesis of skin squamous cell carcinoma(SCC). Methods:TIG2 protein and mRNA in normal skin tissues,uninvolved skin and SCC lesions were detected by immunohistochemistry and hybridization in situ. Results:TIG2 protein and mRNA were expressed in all layers of normal and uninvolved epidermis. In addition, TIG2 expression could be observed in the corneum,granular layers and the upper or middle layers of stratum spinosum of the marginal part of SCC lesions. Furthermore,TIG2 positive expression could only be observed slightly around the keratin pearls of SCC Ⅰ~Ⅱ stages,and no expression in SCC Ⅲ~Ⅳ stages. TIG2 expression is less in aggressive SCC compared with that in the normal skin and uninvolved tissues from SCC(P < 0.01); and TIG2 expression is absent in the basal layer of marginal epidermis of SCC compared with that in normal skin and uninvolved tissues from SCC patients(P < 0.01);moreover,TIG2 expression is also less in SCC Ⅲ~Ⅳ than in the marginal tissues of SCC(P < 0.01). Conclusion:TIG2 may play an important role in the pathogenesis of SCC.

Abstract:Objective:To investigate expressions of human ZRT/IRT-like protein(hZIP1) and cation diffusion facilitator(CDF,ZnT1) mRNAs, study the relationship between Zinc,Zinc transporters and leukemia, and understand its application at precaution,diagnosis and therapy of leukemia. Methods:the experiment objects were divided into the acute myelocytic group, chronic myelocytic leukemia, control group and cell line group. Expressions of hZIP1 and ZnT1 mRNAs were analyzed by RT-PCR. Zinc level in supernatant of bone marrow were measured by flame atomic absorption spectrometry(FAAS) expression. Results:Expression of hZIP1 mRNA of the mononuclearcell in bone marrow was detected. There was no significant difference among incipient group,relapsed group,complete remission group and control group(P > 0.05). Expression of ZnT1 mRNA of the mononuclearcell in bone marrow was detected,and its expression in incipient group and relapsed group were remarkably higher than those in complete-remission group and control group(P < 0.05). The results in cell strains, K562 and HL60, accorded with those in clinical collections. The Zinc levels in supernatant of bone marrow in incipient group and relapsed group were remarkably higher than those in control group and complete remission group(P < 0.05). Conclusion:The hZIP1 plays role in regulating Zinc balance of cells inside and outside,but it has no significant relevance with Zinc regulation when leukeamia occurs. ZnT1 is a significant gene in regulating Zinc level, and has significant relevance with Zinc regulation of the mononuclearcell in bone marrow when leukeamia occurs. The expression of ZnT1 in the bone marrow and the Zinc level in supernatant of bone marrow decreased after therapy suggesting the improvement condition of patients.

Abstract:Objective:To investigate the effect of recombinant human erythropoietin(rhEPO) on the hemoglobin(Hb) levels of anemic malignancy patients receiving chemotherapy. Methods:151 malignancy patients who received chemotherapy were randomly divided into 3 groups: the common therapy group,received rhEPO 10 000 U three times per week for 8 weeks;the high-dose induction therapy group, received rhEPO 20 000 U s.c. per day on d3~d8,and then rhEPO 10 000 U s.c. three times per week;the control group,received other managements similar to rhEPO therapy. Results:The mean Hb level of the high-dose group increased by 2.6 g/L in two weeks with the therapy of rhEPO(P = 0.000),and higher than that of the common therapy group by 2.9 g/L,but there was no statistic significance in Hb between the two groups(P = 0.053); On the 3rd and 4th week,the mean Hb level of high-dose group reached 3.8 g/L and 3.4 g/L, higher than that of the control group(P = 0.048 and 0.029, respectively);the high-dose group, compared with the common group, had a higher level of Hb at the 5 th,6 th,7 th and 8 th week, although they had no statistic difference in Hb levels(P > 0.05). Compared with that of the control group,the mean Hb level of rhEPO therapy group had more steadily increased since the 3rd week(P < 0.01). Conclusion:rhEPO can effectively treat the anemic malignancy patients receiving chemotherapy. Compared with the common therapy,the high-dose therapy can more quickly improve the mean Hb level of malignancy patients, but both of them have no significant difference in later period.

Abstract:Objective:To evalate the effect of remifentanil(0.3 μg/kg) bolus on the efficacy of propofol during target-controlled infusion(TCI) of propofol anesthetized analgestic artificial. Methods:Forty-two young patients undergoing painless artificial abortion were randomly allocated to two groups:propofol group(group P,n = 20) and propofol plus remifentanil group(group RP,n = 22). Patients were sequentially given different doses propofol so that the 50% target propofol plasma concentration(Cp50 which there is a 50% chance that patients show no movement in response for dilatation or curettage of cervix and uterine aspiration) could be determined by up-and-down sequential test. Anesthetic depth was observed by S/5TM Entropy. Heart rate(HR),mean arterial pressure(MAP),pulse oxygen saturation(SpO2),state entropy(SE) and response entropy(RE) were continuously monitored during anesthesia. Modified Observer’s Assessment of Alertness/Sedation scale(MOAA/S) was used to detect the level of consciousness. Comparison of MOAA/S with RE and SE was performed with the Spearman correlation coefficient. Results:Propofol consumption and target propofol plasma concentration(Cp)were less than those in group P(P < 0.01). Cp50 of cervix dilatation was 3.495 vs. 4.637μg/kg in the group RP and group P. Cp50 of cervix dilatation was 4.417 vs. 5.825 μg/kg in the group RP and group P. RE and SE of group RP were significantly higher than those of group P(P < 0.01 or P < 0.05) during operation but there were similar in the both groups during awaken time. MOAA/S was well positively correlated to RE and SE(P < 0.01) in both groups. Conclusion:In young female patients,0.3 μg/kg remifentanil bolus can effectively decrease Cp50 values for TCI propofol and depth of anesthetic sedation,but it can not influence the ability of Cp and spectral entropy to predict and detect MOAA/S during painless artificial abortion.