Abstract:Objective:To study regulation mechanism of GnRH agonist(GnRHa) on testosterone synthesis in rat Leydig cells. Methods:The primary rat Leydig cells were cultured 24 h in vitro, and then treated with GnRHa (10-7 mol/L) at varied time courses (6,12,24,36,48 h);the expression of 3β-HSD protein was analyzed by Western blot. After stimulation with GnRHa and PD98059 (50 μmol/L),the specific inhibitor of ERK1/2,in Leydig cells,the expression of 3β-HSD and the level of testosterone were also investigated. Results:Treatment with GnRHa resulted in 3β-HSD expression in a time-dependent manner. 3β-HSD expression was significantly increased 45% over control after 12 h of treatment with GnRHa (P < 0.05),maximally elevated 1.0-fold over control at 24 h (P < 0.05),and then returned to basic level after 48 h. We also found the activation of 3β-HSD induced by GnRHa was markedly reversed in the presence of PD98059 (61% compared with GnRHa group,P < 0.05),following the significant decrease of testosterone (45% compared with GnRHa group,P < 0.05). Conclusion:The results suggest that GnRHa regulates the expression of 3β-HSD through ERK1/2,and further induces testosterone synthesis.

Abstract:Objective:To determine effects of parathyroid hormone (PTH) and different dietary calcium and phosphate on synthesis of active vitamin D. Methods:Two-month-old wild-type(WT) and PTH null (PTH-/-) mice had been fed on a normal diet (1% calcium,0.64% phosphate),high calcium low phosphate diet(2% calcium,0.4% phosphate) or low calcium low phosphate diet (0.005-0.01% calcium,0.4% phosphate) for 4 weeks,and serum calcium,phospharus,PTH and 1,25(OH)2D3 levels were measured. The expression of renal 25-hydroxyvitamin D 1α-hydroxylase[1α(OH)ase]gene was assessed using RT-PCR. Results:Serum calcium levels were decreased,serum phospharus levels were raised. The expression of renal 1α(OH)ase gene was up-regulated,whereas serum 1,25(OH)2D3 levels were reduced in PTH-/- mice on the normal diet compared with their WT littlemates on the same diet. Serum PTH and 1,25(OH)2D3 levels were reduced and the expression of renal 1α(OH)ase gene was down-regulated in WT and PTH-/- mice on the high calcium low phosphate diet compared with genotype-matched mice on the normal diet. Serum calcium and phospharus levels were normal,and the expression of renal 1α(OH)ase gene was still up-regulated with same level of 1,25(OH)2D3 in PTH-/- mice on the high calcium low phosphate diet compared with their WT littlemates on the same diet. Serum PTH and 1,25(OH)2D3 levels were raised significantly and the expression of renal 1α(OH)ase gene was up-regulated dramatically in WT and PTH-/- mice on the low calcium low phosphate diet compared with genotype-matched mice on the normal diet. Serum calcium levels were reduced more dramatically,and serum phospharus levels were less higher,with expression of renal 1α(OH)ase gene and lower serum 1,25(OH)2D3 levels in PTH-/- mice on the low calcium low phosphate diet compared with their WT littlemates on the same diet. Conclusion:Synthesis of active vitamin D was regulated by dietary calcium and phosphate in both PTH-dependent and PTH-independent manners.

Abstract:Objective:To investigate the effects of Resveratrol on the renal hypertrophy in early diabetic rats and the possible mechanism. Methods:A diabetic state was induced in 13 Spargue-Dawley rats by a single intraperitoneal injection of streptozotocin and then raised for 10 weeks. Six normal rats served as control (NC group). Diabetic rats were randomly divided into diabetic group (DM,n=7) and Resveratrol group (DR,n=6). Rats in DR group had been treated with Resveratrol 10 mg/(kg·d) intragastricly for 4 weeks. At the end of the experiments,the blood glucose (BG),body weight (BW),24 h urinary albumin excretion,serum creatinine of three groups were measuerd. Renal cortex was stained with hematoxylin and eosin. The expression of phosphorylation of 4E-BP1 protein was examined by immunohistochemistry. The expression of phosphorylation of S6 protein in the renal cortex was determined by Western blot. Results:The BW of rats in DM and DR groups decreased significantly,and the BG in DM and DR groups increased significantly compared with that in NC group (P < 0.01),and the BG of diabetic rats treated with Resveratrol improved compared with that in DM group (P < 0.05). Compared with normal rats,24 h urinary albumin excretion and serum creatinine significantly elevated in the DM and DR group (P < 0.01). 24 h urinary albumin excretion in DR group decreased significantly compared with that in DM group (P < 0.05). Resveratrol treatment attenuated glomerula mesangial cells hyperplasia,and reduced glomerula mesangial field enlargement and glomerula volume. The expressions of phosphorylation of 4E-BP1 and S6 protein in DM and DR groups were much higher than those in NC group (P < 0.01),but they were comparatively much lower in the DR group than those in DM group (P < 0.01). Conclusion:Resveratrol treatment can prevent the renal enlargement in early diabetic rats and thus halt the early step of the development of diabetic nephropathy. The mechanism is that Resveratrol may inhibit the phosphorylation of mTOR signal pathway downstream protein 4E-BP1 and S6.

Abstract:Objective:To explore the relationship between HLA-A alleles and acute-onset autoimmune diabetes in Jiangsu area. Methods:64 patients with acute-onset ketotic and insuln-depdent diabetic in Jiangsu area were recruited. Peripheral blood cell genomic DNA was extracted from,and detected using polymerase chain reaction restriction sequence specific oligonacleotide(PCR-SSO) technology,and the HLA-A alleles frequencies were studied. Results:The sorts of HLA-A alleles are not different between acute autoimmune diabetes group and healthy control group. The frenquencies of HLA-A*03 and HLA-A*24 alleles in the patients were significantly higher than that in healthy control group(P < 0.05). The frenquencies of HLA-A*01,HLA-A*11,HLA-A*31 alleles in the patients with acute autoimmune diabetes were significantly lower than those in the healthy control group(P < 0.05). Conclusion:HLA-A*03 and HLA-A*24 alleles might be susceptible genes of acute autoimmune diabetes in Jiangsu area. A*01-HLA-A*11-HLA-A*31 might be protective genes.

Abstract:Objective:To investigate the significance of arterial reconstruction on the model of 40% small-for-size orthotopic liver transplantation in rats. Methods:Modified two-cuff technique was applied to establish a rat model of 40% orthotopic liver transplantation. A total of 192 sprague dawley (SD) rats were randomly divided into 2 groups:small-for-size orthotopic liver transplantation with arterial reconstruction group (A group) and small-for-size orthotopic liver transplantation without arterial reconstruction group (B group). One week survival rate,main indexes of liver function,histology and the expression of the proliferative cell nuclear antigen (PCNA) of liver graft by immunohistochemical method were detected on the 1st,2nd,4th and 7th day after transplantation,respectively. Results:One week survival rate of A group and B group were 66.7% and 50.0%,respectively (P > 0.05). Alanine aminotransferase (ALT) and total bilirubin(TB) began to elevate from the first day and peaked on the second day after surgery. ALT in B group on the second and fourth day were higher significantly than those in A group(P < 0.05). TB in B group on the second and seventh day were higher significantly than those in A group(P < 0.05). Histological findings indicated that more diploid and polyploid hepatocytes could be seen in A group. The expression of PCNA of liver graft peaked on the 2nd day after surgery. The expression of PCNA in A group was higher on the 1st day and lower on the 7th day than that in B group after operation(P < 0.05). Conclusion:Arterial reconstruction can improve liver function of liver grafts after small-for-size orthotopic liver transplantation,and alleviate the histological changes and promote the regeneration of liver grafts quickly.

Abstract:Objective:To explore the transfecting EGFP genes effect into cells on adherence ability of cells to biomaterials,which influences material biocompatibility evalution. Methods:EGFP gene was successflly delivered into osteoblasts induced from human marrow mesenchymal stem cells(MSCs) through electroporation transfection,and the osteblastes were seeded into tissue engineering bone with biomaterials. All cultured cells were detected by inverted microscope,scanning electron microscope,and fluorescent microscope. Results:It was observed that the osteoblasts transfected with EGFP gene had been successfully adhered to tissue engineering bone,and compared with osteoblasts without EGFP gene transfection,there was no obvious difference. Conclusion:It is suggested that transfection of GFP marker does not harm the biocompatibility to biomaterials and proliferative activity of osteoblasts induced from MSCs,which is helpful to understanding biocompatibility of materials accurately.

Abstract:Objective:To evaluate the expression of CD44s and CD44v6 in colorectal carcinoma and its clinical significance. Methods:The expressions of CD44s and CD44v6 in tumor tissue were detected by immunohistochemically in 57 patients with colorectal carcinoma. Results:The positive rates of CD44s and CD44v6 were 66.7% and 63.2%,respectively. There was significant correlation between CD44s immunoreactivity and Duke＇s stage,the grade of tumor (P < 0.05,Spearman correlation test). Significant correlation between CD44v6 immunoreactivity and the grade of tumor,Duke’s stage,lymph node metastasis was also noticed (P < 0.05,Spearman correlation test). The 5-year survival rates were 78.95% and 55.26% in CD44s negative cases and CD44s positive ones,respectively. The difference between the two groups was not significant (P > 0.05,Log-rank test). As CD44v6,the 5-year survival rates were 80.95% and 52.78% in CD44v6 negative cases and CD44v6 positive ones,respectively.The difference between the two groups was significant (P < 0.05,Log-rank test). In multivariate analysis using the Cox regression model,CD44v6 expression emerged as an independent prognostic indicator. Conclusion:CD44s and CD44v6 might play important roles in metastasis of colorectal carcinoma,and the expression of CD44v6 might be a new useful independent prognostic marker of colorectal carcinoma.

Abstract:Objective:To investigate the effect of liver resection on regulatory CD4+CD25+T lymphocytes (Treg) in peripheral blood and its clinic significance in for patients with hepatocellular carcinoma(HCC). Methods:Flow cytometer was used to analyze the percent of Treg in peripheral blood mononudear cell before and affer surgery,and the ratio of Treg to PBM in tumor tissue and normal liver tissue,enzyme-linked immunosorbent assay (ELISA) was used to detect the level of TGF-β,IL-10 in tumor tissue and normal liver tissue. Results:The percentage of Treg in PBM and tumor tissue of HCC patients were(13.98 ± 3.03)%,(17.74 ± 4.12)%,respectively,both were higher than those in the normal group,and the percentage of Treg in phase Ⅲ-Ⅳ patients was higher than that in phase Ⅰ-Ⅱ(P < 0.05). One month after liver resection,the Treg significantly reduced in the PBM compared before operation (9.87±2.78)% (P < 0.05). TGF-β and IL-10 in tumor tissue were significantly higher than those in normal liver tissue. Conclusion:The level of Treg in patients with HCC significantly increased,which was positive correlated with TNM classification. Liver resection can significantly reduce the proportion of Treg,for which may be related with that the removed tumor micro-environment has high level of Treg,TGF-β and IL-10.

Abstract:Objective:To investigate the expression and significance of HepPar1,armor embryo protein(AFP),CD34,CD10 and CEA in hepatocellular carcinoma(HCC) with negative serum AFP. Methods:The expression of HepPar1,AFP,CD34,CD10 and CEA was tested by immunohistochemistry in 100 HCC samples with negative serum AFP. Results:In HCC with negative serum AFP,the expression rate of HepPar1 was 86%,and had close correlation with the differentiation,structure type,cytological subtypes and the number of lymphocyte around the tumor;the expression rates of AFP and CD34 were 18% and 87% respectively,and had both close correlation with the number of caryocinesis and cytological subtypes;the expression rate of CD10 was 41%,and had close correlation with the cytological subtypes and gender;the expression rate of CEA was 71%,but had no correlation with the clinical features. Conclusion:Unique immunohistochemical characteristics exist in HCC with negative serum AFP.

Abstract:Objective:To detect the effects of carbaryl exposure on sperm DNA damage,and explore the mechanism of carbaryl induced sperm DNA damage. Methods:A case-control analysis was conducted,including 31 male carbaryl exposure workers (exposure group),36 male administrators in the office in a pesticide factory (internal control group) and 22 male administrators in same area (external control group). The activity of semen SOD,the level of sperm ROS and the sperm apoptosis in three groups were compared. In cytotoxic experiments,GC2-spd cells were cultured with different doses of carbaryl. The cell survival rate,apoptosis rate,the production of ROS and the activity of SOD were measured. Results:The sperm DNA apoptosis and the level of sperm ROS in the exposure group were significantly higher than those in the internal and external control groups (P < 0.05). In addition,a negative correlation between the sperm apoptosis and semen SOD was found (r=-0.53,P < 0.001),while there was a positive correlation between the sperm apoptosis and sperm ROS (r=0.32,P = 0.002). Cytotoxic experiments showed that cell apoptosis rate and production of ROS increased,while the cell survival rate and the activity of SOD decreased at higher dose of carbaryl. Conclusion:Our findings suggested that occupational exposure to carbaryl production can cause the sperm DNA damage by oxidative stress.

Abstract:Objective:To describe the current status of patients’ adherence to anti-tuberculosis medications and identify risk factors associated with treatment interruption in Jiangsu,and provide evidences to explore intervention measures. Methods:13 counties (districts) were randomly sampled as study sites. On each site,60 sputum smear-positive tuberculosis patients registered since January 1,2006 were selected as study subjects and interviewed by a structured questionnaire. Risk factors associated with non-adherence were analyzed. Results:82 of 670 enrolled pulmonary tuberculosis patients interrupted treatment,accounting for 12.2%. Drug side effect was the most important factor causing patients non-adherence(37.8%). Patients’ education and the way of taking medicine were the main influence factors affecting patients’ adherence. Conclusion:More importance should be attached to treatment adherence under the current DOTS strategy. Synthetic measures taken to focus groups can reduce the risk for interruption and increase cure rate.

Abstract:Objective:To understand the situation of Ice(metamfetamine) abuse in Jiangsu province in 2008. Methods:An investigation and analysis were conducted among 1 029 Ice abusers who were under treatment in Jiangsu detoxification settings or captured by drug control units in 2008 with the Drug Abuse Monitor Survey Questionnaire designed by National Surveillance Center of Drug Abuse. Results:Among the 1 029 Ice abusers,male accounted for 82.70%,while female 17.30%. The average age was 29.73±7.32 with male 30.81±7.10 and female 24.55±6.03. Among those aged under 25 years,male accounted for 25.14%,while female 65.73%. It showed significant difference(P < 0.01) compared male with female in the group aged under 25. For the education level,79.01% of the abusers had middle or senior education. 52.58% was single. 72.79% had no job. 1.76% was female while 16.85% male among the poulation worked in creation sites,which also showed significant difference(P < 0.01). The main reason for abusing Ice was satisfying curiosity(64.92%). Peers(65.60%) and creation sites(30.81%) were the main sources for them to get Ice. Temporary living places,hotels,rented houses(51.41%) were the main places Ice abuse took place. The average money spent per month by 52.28% abusers was 2 000~8 000 RMB. 64.33% abusers were single users with 85.23% chasing dragon. Conclusion:Ice abusers have obvious social demographic and behavioral characteristics in Jiangsu province. The results suggest that drug abuse education and prevention should be strengthened on young people especially women,and administration for temporary living places,hotels and rented houses,creation sites also should be enhanced.

Abstract:Objective:To detect HIV-1 infection in window period and estimate HIV-1 incidence among men who have sex with men(MSM). Methods:Pooled HIV-1 RNA RT-PCR(3-staged pooling by 20∶1,5∶1,1∶1) was conducted and HIV-1 RNA copies were quantitated using COBAS AMPLICOR HIV-11.5 version MONITOR Test. Results:The plasma samples from 948 MSM,which were negative by HIV third-generation antibody assay were tested. Four samples were found to be HIV-1 RNA positive,and acute infection was diagnozed with seroconversion during follow up. The HIV-1 incidence was 5.55% per year[95%CI,3.50%~7.59%]. Conclusion:Pooled HIV-1 RNA RT-PCR is a good method of testing,and can be applied to screen HIV infection in winow-period in high-risk groups.

Abstract:Objective:To investigate the epidemiologic characteristics of syphilis in Jiangsu province from 2004 to 2008 and to provide scientific reference for further control of the disease. Methods:Syphilis cases reported in China Information System for Disease Control and Prevention in Jiangsu province were collected and the data were analyzed epidemiologically. Results:The incidence rate of syphilis increased rapidly in Jiangsu province from 10.59 per 100 000 residents in 2004 to 28.68 per 100 000 residents in 2008,and the average annual increase of incidence rate and reported case of syphilis were 28.42% and 28.68% in recent 5 years,respectively;primary and secondary syphilis cases were 69.75% in all syphilis cases;most patients were 20~39 years old,which accounted for 51.55%;the majority of syphilis cases were reported in the south of Jiangsu province. Conclusion:The syphilis epidemiology in Jiangsu province is increasing rapidly,and a series of measurements on strengthening the prevention and cure on STD and HIV, and standarization treatment of syphilis must be taken to control the prevalence of syphilis.

Abstract:Objective:To understand the current situation of the network on Voluntary HIV Conselling and Testing (VCT) service and its effectiveness for proposing reasonable strategies on effective implementation of the“Four Frees and One Care”policy. Methods:Data from VCT clinics distributing in health care settings at different level from 2005 to 2007 were collected for analysis. Results:VCT clinics increased from 80 in 2005 to 307 in 2007,the numbers of HIV testing were 5 402,53 149 and 49 012,respectively,over the three years. Among attendance,the majority experienced heterosexual intercourse,while people who were homoerotism or drug users were less possibility to seek the service. Screening positive cases mainly came from heterosexual intercourse,homosexual intercourse and injecting drug use. VCT has similar positive detecting rate compared with sentinel surveillance system,but much higher than mass screening system. However,the annul fund for VCT was lowest. Conclusion:The coverage of VCT clinics in Jiangsu province has met national requirements. The establishment of the VCT network is cost-effective,and is creating effectiveness for HIV/AIDS prevention and control.

Abstract:Objective:To understand the infection rate of HCV in drug users(DUs) and figure out the risk factors related to HCV infection,in order to provide basis for prevention and control in the particular targeted population. Methods:DUs,who were arrested from April to June,had been randomly sampled at 7 treatment centers and camps from 2005 to 2008. And a total number of sampled DUs was 2 811. Blood was collected for HCV antibody testing by ELISA assay after face to face interview. The data were checked by EpiData 3.1 software before the establishment of databank,and analysised by SPSS 11.5. Results:Among the 2 811 DUs,957 of them were HCV positive(34.0%),and the positive rate in male and female were 35.1% and 29.2%,respectively. Men showed higher positive rate than women( -字2=6.32,P < 0.05). The age range of participating was 18~74 years old. The positive rates were different in various groups and the highest rate was found in the 30~39 years old group. Significant inter-group difference was found(-字2=65.31,P < 0.05),but no significant difference was figured out among DUs between Jiangsu province and others,or DUs in various marital status groups. Drug used by injection,sexual behavior for money or drug,and sexual behavior without condom used were high risk factors for HCV infection. Conclusion:The positive rate is much higher in DUs than normal population,which suggests that the special group has risk factors for HCV epidemiology,and should pay more attention in prevention and control of HCV infection. More targeted integrated measures to DUs on health education and behavioral intervention should be performed for prevention and control.

Abstract:Objective:The aim of the study is to make a full understanding of military cadets’ psychological condition and the related elements so as to provide evidences for psychological health education. In the meantime,the analysis could help give pertinently the psychological consultation to the cadets. Methods:SCL-90 self-rating symptom scale and Eysenck personality questionnaire (EPQ) were applied to test 600 freshmen of a military academy. Compared the results with some regular parametres,the differences among various groups were analyzed. Results:The number of cadets with positive criterion≥160 was 45,which was 8.8% of the total number. The psychological condition of the freshmen from different hometowns was remarkably different. The SCL-90 factors of cadets are lower than the factors of local youth,military servicemen and local university students. The first five factors of the cadets’ psychological problems were compulsion,personal relationship,somatization factor,anxiety and depression. The cadets’ psychological condition was connected with their internal and external characters and also with their nerves. Conclusion:The general condition of the military cadets’ psychology is comparatively good. It is influenced by various factors like family background,society and is closely related with the character of the cadets. We must build up proper psychological education system according to the real condition of the cadets in the academy through which the military cadets’ comprehensive quality might be effectively improved.

Abstract:Objective:To compare the difference of serum 1,25-dihydroxyvitamin D3(1,25(OH)2D3) between type 2 diabetes mellitus patients and normal persons,and to analyze the relationship between vitamin D3 and type 2 diabetes mellitus. Methods:110 patients with type 2 diabetes mellitus were selected as the case group,and 105 healthy people were selected as the control group. ELISA was used for the detection of serum 1,25-dihydroxyvitamin D3. Questionnaire survey was performed,and clinical and biochemical indexes were also detected. Results:There was a significant difference in serum 1,25(OH)2D3 level between the case and control group (P < 0.01). There was a significant negative correlation between serum 1,25(OH)2D3 level and fasting blood glucose(rs=-0.696,P < 0.01). Conclusion:Serum 1,25(OH)2D3 in the case group was significantly lower than that in the control group. The higher fasting blood glucose,the lower serum 1,25(OH)2D3.

Abstract:Objective:To investigate the relationship of serum ferritin and insulin resistance in patients with non-alcoholic fatty liver disease(NAFLD) alone and NAFLD combined with type 2 diabetes. Methods:A total of 41 with NAFLD alone,76 NAFLD patients with type 2 diabetes and 60 healthy controls were studied. Abdominal ultrasound was taken for fatty liver examination. Serum ferritin and insulin levels were determined by radioimmunoassay(RIA). Insulin resistance indexes were assessed by the homeostasis assessment-insulin resistance(HOMA-IR). Other biochemical variables were measured by routine methods. Results:Compared with healthy controls, the BMI,ALT,AST,TC,LDL,FBG,FINS,HOMA IR,HbA1C,UAlb/UCr and SF significantly increased in NAFLD patient with type 2 diabetes. Compared with NAFLD patients, insulin resistance was more serious in NAFLD with type 2 diabetes. Correlation analysis showed FBG,HOMA IR and HbA1C were closely related with SF. Conclusion:Serum iron metabolism is closely related with insulin resistance. Therefore,reduce of insulin resistance and correction of SF are important to prevent NAFLD.

Abstract:Objective:To observe the effects of early intensive statin therapy on inflammatory factors and prognosis in patients with acute coronary syndrome(ACS). Methods:Total 70 patients with ACS were randomly divided into two groups:group A contained 35 patients administered with 10 mg/d atorvastatin besides of routine treatment,group B contained 35 patients administered with 40 mg/d atorvastatin besides of routine treatment. Before and after the treatment for one week,the plasma levels of inflammatory factors (hs-CRP,SAA,MMP-9,Fib) and blood lipid(TC,TG,LDL-C,HDL-C) were detected. All the patients were followed up for 60 days,and the incidence of cardiovascular disease and the adverse reaction of drug were detected. Results:Blood lipid levels had no significant changes in the two groups after treatment for one week(P > 0. 05). Except Fib,the inflammatory factors of hs-CRP,SAA and MMP-9 decreased significantly in group A after treatment with atorvastatin for one week(P < 0.05),and all the four inflammatory factors decreased significantly in group B(P < 0.05),and the changes were more significant in group B than that in group A (P < 0.05). The incidence of cardiovascular disease in group B was less than that in group A markedly (P < 0.05). No severe adverse effects were found in two groups. Conclusion:Early intensive statin therapy could reduce the levels of inflammatory factors,promote the stabilization of atherosclerotic plaque,reduce the incidence of cardiovascular disease and significantly improve prognosis of patients with ACS. Early intensive statin therapy has good effects and safety for patients with ACS.

Abstract:Objective:To investigate the change of the airway oxidative stress marker,8-isoprostane(8-isoPG),and its clinical significance for patients with obstructive sleep apnea hypopnea syndrome(OSAHS). Methods:40 OSAHS patients and 30 normal controls were recruited in the study. Exhaled breath condensate(EBC) and serum 8-isoPG were assayed by ELLSA. 20 OSAHS patients were assayed again after three months’ treatment. Results:①The concentrations of 8-isoPG in EBC were (13.08 ± 1.42)pg/ml before sleep vs (14.93±1.39)pg/ml after sleep in 40 OSAHS patients(P < 0.01),and (11.06±0.72)pg/ml before sleep vs (10.97±0.7)pg/ml after sleep in 30 normal controls (P > 0.05). The concentrations of 8-isoPG in EBC after sleep were significantly higher than those before sleep in 40 OSAHS patients (P < 0.01). ②The serum levels of 8-isoPG were significantly higher in 40 OSAHS patients (36.59 ±14.89) pg/ml than those in 30 normal controls (19.91±7.76) pg/ml (P < 0.01). ③The concentrations of 8-isoPG in EBC of OSAHS patients after sleep were positive correlated with serum 8-isoPG levels (r=0.685,P < 0.01). ④After three months’ treatment with either auto continuous positive airway pressure (Auto-CPAP)or uvulopalatopharyngoplasty(UPPP),there was a significant decrease in the concentrations of 8-isoPG in both EBC and serum of 20 OSAHS patients before sleep and after sleep(all P < 0.01). ⑤ The concentrations of 8-isoPG in EBC of OSAHS patients after sleep were positive correlated with AHI,neck and waist circumferences(r=0.65,0.43 and 0.34,all P < 0.05)and negative with PaO2 (r=-0.31,P < 0.05). Conclusion:These findings suggest that oxidative stress is characteristic of OSAHS patients. The levels of 8-isoPG in exhaled breath condensate depend on the severity of the OSAHS and may prove useful in monitoring of airway oxidative stress of OSAHS.

Abstract:Objective:To evaluate the significance of interferon-inducible protein 10 (IP-10) level in the pleural effusion for the diagnosis of tuberculous pleurisy. Methods:Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the level of IP-10 in 43 patients with tuberculous pleurisy and 33 patients with pleural effusion associated with malignant tumor. The receiver operating characteristic (ROC) curves were used to determine the cut-off value and assess the sensitivity,specificity and diagnostic efficiency. Results:The concentration of IP-10 was significantly higher in tuberculous pleurisy (1 095.67 ± 556.29 pg/ml) than in malignant pleurisy (121.39±61.21 pg/ml),(P < 0.01). When 194.5 pg/ml was used as cut-off value,the sensitivity and specificity for IP-10 in diagnosis of tuberculous pleurisy were 83.7% and 97.0%,respectively. And the diagnostic efficiency of IP-10 was similar to that of IFN-γ. Conclusion:Detection of IP-10 in the pleural effusion is helpful in the diagnosis of tuberculous pleurisy.

Abstract:Objective:To establish a LC-MS/MS method for assessment of pharmacokinetics of dispersible tablet of roxithromycin and ambroxol hydrochloride (each containing roxithromycin 150 mg,ambroxol hydrochloride 30 mg). Methods:Each of 12 healthy Chinese volunteers was given a single oral dose of one tablet. Plasma samples were collected at 72 h post-administration and the concentrations were determined by LC-MS/MS. The pharmacokinetic parameters were calculated by DAS software. Results:The main pharmacokinetic parameters of Cmax,Tmax,t1/2z,MRT,AUC0-τ,CLz/F and Vz/F for roxithromycin were (6.99±1.55) μg/ml,(2.04±0.94) h,(13.30±2.56) h,(14.31±2.51) h,(84.56±26.20) h·μg/ml,(1.88± 0.51)L/h and (35.06±8.68)L,respectively;and those for ambroxol hydrochloride were (53.91±22.41) ng/ml,(1.96±0.66) h,(8.24±2.01) h,(7.96±0.47) h,(447.98.±138.95) h·ng/ml,(61.85±14.79)L/h and(724.42±214.86)L,respectively. Conclusion:The established LC-MS/MS method is selective,sensitive and reliable for the pharmacokinetic study of the dispersible tablet of roxithromycin and ambroxol hydrochloride.

Abstract:Objective:To investigate the stress distribution on the post of a cast alloy post-core system by three-dimensional finite element analysis. Methods:A three-dimensional finite element analysis was performed. A simulated post/core/crown restoration was constructed on conventional tooth preparations with various diameter of posts and 2.0 mm ferrules. The crown was loaded with a simulated 100 MPa force at 60° angle with tooth longitudinal axis on the palatal surface of the crown. Von Mises stress within the post structure was recorded and analyzed. Results:In the model the Von Mises stress on the top of the post was much larger than that of the tip of the post. Conclusion:The study confirms that the figure of the top of the post should be column,and the diameter of the post in the tip part should be decreased as soon as possible.

Abstract:Objective:In order to identify the relationship between oral lichen planus (OLP) and candida albicans (CA),the effects of home-made Chinese-medicine solutions on controlling the prevalence of CA in patients with OLP,and the toxicity of CA isolates were studied. Methods:In our research,3 groups were as follows:patients with erosive OLP,patients with non-erosive OLP and healthy control,and each group had 100 cases. Before and after administration,concentrated rinse culture was used by Sabouraud plate,and combined with germ tube test,chlamydospore formation test and temperature test in order to isolate and identify the type of candida. The separated and purified CA was inoculated on blood agar and cultured in anaerobic environment,and was its hemolytic activity detected. Results:Before the treatment,the prevalence of candida and colony forming unit (CFU) in erosive OLP group (66%)were significantly higher than that in non-erosive OLP group (26%) and control group (12%) (P < 0.01);after using traditional Chinese medicine(TCM) solution on chlorhexidine solution,for 7 days the prevalence of CA significantly decreased,and there was more significant decreased in the group using TCM solution than that in the group using chlorhexidine solution (P < 0.01). Before the treatment,hemolysis was detected in all the CA cultured plate after 48 hours,and the hemolysis activity significantly decreased after the use of solution. And the decrease was more significant in the group using TCM solution than that in the group using chlorhexidine solution(P < 0.01). Conclusion:The prevalence of candida and CFU were significant in the group with erosive OLP,especially the prevalence of pathogenic CA. It indicates the occurrence and development of erosive OLP are associated with the infection of CA,and the TCM can reduce the prevalence of CA in OLP patients and the amount of CA in oral cavity of OLP patients and inhibit the toxicity of CA. The TCM solution may have anti-fungal activity in OLP patients.