Abstract:Lung cancer is the most common cancer in the world. It is a highly lethal disease in women and men, and new treatments are urgently needed. Several studies have implicated a role of estrogens and estrogen receptors in lung cancer progression. This review will investigate the biological significance of estrogens in lung cancer cells, the expression and molecular mechanisms of estrogen receptors(ERαand ERβ, elucidate the prognostic significance of estrogens and their receptors in lung carcinomas and provide new options for patients afflicted with lung malignancies.

Abstract:Vascular cognitive impairment(VCI) encompasses the entire range of cognitive deficits associated with cerebrovascular disease(CVD), from mild deficits with little or no functional impairment, such as vascular cognitive impairment-no dementia(VCIND), to full-blown vascular dementia(VaD). Accurate diagnosis of vascular cognitive impairment is important but may be difficult. In this review we report advances in VCI in the following areas: etiology, subtypes, neuropsychology, biomarkers, neuroimaging, and diagnostic criteria.

Abstract:The enzyme, dipeptidyl peptidase Ⅳ(DPP Ⅳ), is a novel target for the treatment of type 2 diabetes. Dipeptidyl peptidase Ⅳ inhibition improves the impaired insulin secretion and decrease postprandial concentrations of glucagon by enhancing the incretin hormone levels lucagon-like peptide-1(GLP-1) and glucose-dependent insulinotropic polypeptide/gastric inhibitory polypeptide(GIP). Recently, DPP Ⅳ inhibitors have attracted more and more attention, several of which have entered pre-clinical and clinical trials, and one has received approval for use as an anti-diabetic agent. Among the DPP Ⅳ inhibitors, two leading agents(sitagliptin and vildagliptin) have been shown to be effective in reducing glycosylated hemoglobin(HbA1c) and fasting plasma glucose(FPG) in patients with type 2 diabetes. This review summarizes the evidence supporting DPP IV inhibitors as potential antidiabetic agents.

Abstract:Objective: We have previously found that mbr is a regulatory element of the bcl2 gene. The objective of this study is to isolate and identify the proteins binding to the 37 mbr in the 3′-end of the mbr. Methods: Streptavidin magnetic particles were ligated to concatameric oligonucleotides of 37 mbr and incubated with the nuclear extracts of Jurkat cells. The DNA-binding proteins were eluted and then resolved by SDS-PAGE. After silver staining, the protein bands were excised and subjected to MALDI-TOF MS. Results: Several protein bands were detected after the isolation with magnetic particles, and Splicing factor, proline- and glutamine-rich(SFPQ), Poly(ADP-ribose) polymerase 1(PARP), and promyelocytic leukemia protein(PML) were identified by MALDI-TOF MS. Conclusion: Several proteins were isolated and identified from the 37 mbr-protein complex. Results of this study establish a foundation for further study of the mechanisms by which mbr executes its regulatory function.

Abstract:Objective: To investigate the differences of primary culture, purification and biological characteristics between endothelial cells and smooth muscle cells from rat aorta. Methods: Endothelial cells were obtained using the vascular ring adherence, collagenase digestion method and an improved vascular ring adherence method, while smooth muscle cells were separated from tissue sections of rat aorta. Clones of endothelial cells were selected by limiting dilution assay. Both cell types were identified using specific cell immunofluorescent markers, and phase contrast microscopy was used to observe the morphological disparity between endothelial cells and smooth muscle cells at the single cell and colony level. Cell proliferation was determined by the cell counting kit-8. Differences between endothelial cells and smooth muscle cells were evaluated in trypsin digestion time, attachment time and recovery after cryopreservation. Results: Endothelial cells were obtained by all three methods. The improved vascular ring method provided the most reproducible results. Cells were in good condition, and of high purity. Smooth muscle cells were cultured successfully by the tissue fragment culture method. Clonal expansion of single endothelial cells was attained. The two cell types expressed their respective specific markers, and the rate of proliferation of smooth muscle cells exceeded that of endothelial cells. Endothelial cells were more sensitive to trypsin digestion than smooth muscle cells. In addition, they had a shorter adherence time and better recovery following cryopreservation than smooth muscle cells. Conclusion: The improved vascular ring method was optimal for yielding endothelial cells. Limiting dilution is a novel and valid method for purifying primary endothelial cells from rat aorta. Primary rat endothelial cell and vascular smooth muscle cell cultures exhibited different morphological characteristics, proliferation rate, adherence time, susceptibility to trypsin digestion and recovery after cryopreservation. Our research can be a good foundation for further application in the regeneration of blood vessel.

Abstract:Objective: To investigate the expression of OX40 ligand(OX40L) on C-reactive protein(CRP)-triggered mouse aorta endothelial cells (MAECs) in vitro. Methods: MAECs from aorta were isolated by digestion with collagenase typeⅡ. The cell growth was confirmed by morphological characteristics and the immunological marker, factor Ⅷ(or Willebrand factor, vWF). The expression of OX40L by MAECs was detected by RT-PCR and western blot after incubating with 100μg/ml CRP for 48 hours. Results: Twenty-day cultures of MAECs formed confluent monolayer of a cobblestone pattern. RT-PCR and western blot assay showed that the level of OX40L expression in MAECs receiving CRP treatment was higher than control. Conclusion: A reliable method is described to isolate and propagate MAECs. CRP upregulates OX40L expression in MAECs.

Abstract:Objective: This study investigated the effects of rh-TRAIL with or without chemotherapeutic drugs on the apoptosis of the osteosa-rcoma cell line, MG-63, and the influence of chemotherapeutic drugs on changes in the expression of DR5 and YinYang1(YY1) in MG-63 cells. Methods: The effects of treatment with rh-TRAIL alone and/or chemotherapeutic drugs on MG-63 cell growth inhibition and apoptosis were measured using the MTT assay, FACS analysis of Annexin V labeled cells, and the mRNA changes of DR5 and YY1 were detected by RT-PCR. Results: Rh-TRAIL protein inhibited the growth of MG-63 cells, and this inhibition was increased by adriamycin and IFN-γ. Adriamycin and IFN-γ significantly facilitated the induction of the expression of DR5 and reduced the expression of YY1. Conclusion: The apoptosis-inducing effect of rh-TRAIL in MG-63 cells was enhanced by chemotherapeutic drugs.

Abstract:Objective: To construct the point mutation plasmids expressing HCV NS3/4A with different secondary structures at the N-terminus, and to analyze their serine protease activities. Methods: The point mutation plasmid constructs were generated by using the QuickChange site-directed mutagenesis kit with the backbone of M-H05-5 (A1-1), and were named as subgroup A1-2, A2-1, A2-2, B1-1, B1-2, B2-1, and B2-2 respectively. The transient expression of the constructs was investigated by immunofluorescence assay and Western blot analysis. The difference in in cis and in trans NS3 serine protease activity between each subgroup was determined by Western blot analysis. Luciferase reporter assay was used to observe the inhibitory effects of the constructs on RIG-I induced IFN-βpromoter activity and on p53-dependent transcriptional activation. Results: The point mutation plasmid constructs were verified for the correct sequence by DNA sequencing. The immunofluorescence assay revealed 4 subcellular localization patterns of NS3, including dot-like staining, diffuse staining, doughnut-like staining, and rod-shape staining. Western blot analysis indicated that the incomplete cleavage of NS3/4A appeared in subgroups A2-1 and B2-1, indicating that the in cis NS3 serine protease activities of subgroup A2-1 and B2-1 were weaker when compared with the other subgroups. By using NS5A/5BΔC as a substrate for NS3/4A serine protease, it was also found that the in trans NS3 serine protease activities of subgroup A2-1 and B2-1 were also weaker compared the other subgroups. Differences in inhibitory effects of HCV NS3 on RIG-I induced IFN-βpromoter activity and on p53-dependent transcriptional activation were also observed between subgroup A2-1, B2-1 and the other subgroups. Conclusion: The results suggest that subgroup A2-1 and B2-1 has weaker serine protease activities and weaker inhibitory activities on host cell functions than the other subgroups, which might be explained by the different secondary structure of the 120-aa sequence at N-terminus of NS3.

Abstract:Objective: To investigate the effects and underlying mechanisms of different doses of nerve growth factor(NGF) pretreatment on neuron apoptosis and the expressions of the apoptosis-related protein, Bcl-2 and Bax, in the gerbil cerebral prefrontal cortex following global cerebral ischemia-reperfusion(I/R) injury. Methods: Fifty-four gerbils were randomly divided into five groups, group C: sham operation(n = 6); group I/R(n = 12), group L(n = 12): low-dose NGF+I/R, group M(n = 12): medium-dose NGF+I/R and group H (n = 12): high-dose NGF+I/R. Groups I/R, L , M and H were further divided into 2 subgroups according to the duration of reperfusion(24 h and 72 h). The global cerebral I/R injury model was induced by bilateral carotid artery occlusion for 20 min, followed by removal of the clamps to permit reperfusion. In groups L, M and H, NGF was injected into the lateral ventricle 24 h prior to ischemia. Terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL) and immunohis-tochemical staining were performed to detect neuron apoptosis and the expressions of Bcl-2 and Bax protein in the cerebral cortex, respectively. Results: In the I/R group and NGF pretreatment groups(L, M and H groups), reperfusion for 72 h caused higher percentages of both neurons exhibiting apoptosis and Bax positive cells(P < 0.01), but lower percentages of Bcl-2 positive cells compared with the corresponding 24 h reperfusion groups(P < 0.05). All NGF pretreatment groups exhibited lower percentages of neurons exhibiting apoptosis and Bax positive cells but a higher percentage of Bcl-2 positive-cells relative to the I/R group(P < 0.01). Moreover, the high-dose NGF pretreatment group had a greater decreased neuronal apoptosis and Bax protein expression and increased Bcl-2 protein expression than either the low-or medium-dose groups. Conclusion: Neuron apoptosis participates in the progression of cerebral ischemia-reperfusion injury. The protective effect of NGF pretreatment against ischemia-reperfusion-induced neuron apoptosis seems to be both time- and dose-dependent. This anti-apoptosis mechanism may be associated with upregulation of Bcl-2 protein expression and downregulation of Bax protein expression.

Abstract:Objective: To evaluate the efficacy and feasibility of the transradial approach for primary percutaneous coronary intervention (PCI) in patients with acute myocardial infarction(AMI). Methods: 195 patients with acute myocardial infarction were randomly divided into two groups according to the different PCI operation pathways. 105 cases were assigned to the transfemoral artery group and 90 cases to the transradial artery group. We analyzed the data from the two groups, including the achievement ratio of paracentesis, cannulation time, the time from local anesthesia to the first time balloon inflation, the time of the total procedure, achievement ratio of PCI, incidence rate of vascular complications, total duration of hospitalization, and the six-month follow-up results in both groups. Results: Our results showed that the achievement ratio of arteriopuncture, cannulation time and the time from local anesthesia to the first time balloon inflation in the transradial and transfemoral groups were 98.9% vs. 100%, 3.15 ± 1.56 min vs. 2.86 ± 0.97 min, and 18.56 ± 4.37 min vs. 17.75 ± 3.21 min, respectively. These differences between the two groups were not statistically significant. The total operating time was 29.75 ± 4.38 min for the transradial group and 27.89 ± 3.95 min(P < 0.05) for the transfemoral group. The operation achievement ratio in the transradial group was 96.7%, and 96.2% in the transfemoral group. The incidence of puncture point complications was 2.2% in the transradial group and 11.4% in the transfemoral group, and this difference was significant. The duration of hospitalization was 10.56 ± 2.85 days for the transradial group and 13.78 ± 3.15 days(P < 0.05) for the transfemoral group. At the six-month follow-up, the rate of survival without cardiac event was 86.1% vs. 86.4% respectively in the transradial and transfemoral groups(P > 0.05). Conclusion: The transradial approach was as effective as the transfemoral approach, and there were fewer puncture point complications as well as a shorter span of hospitalization in the transradial group. PCI via the transradial approach is safe, effective and feasible in patients with AMI.

Abstract:Objective: To investigate the changes of cyclin L2(CCNL2) gene mRNA and protein during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between CCNL2 gene and the differentiation of cardiac myocytes. Methods: P19 cells were cultured with 0.9% DMSO for 18 days. Western blots of cardiac troponin I (cTnI) were used to identify cell differentiation. Total RNA was extracted from P19 cells during the process of differentiation at various time points:pre-differentiation(Day 0), and Day 1 to Day 18. The expression levels of CCNL2 gene mRNA and protein were evaluated by RT-PCR and Western blot, respectively. Results: After being induced to differentiate by DMSO for 4 days in suspension, spontaneously and rhythmically beating cells were seen at 8 day, which were cTnI-positive. In P19 cells, both the expression level of CCNL2 gene mRNA and protein were gradually down-regulated. Conclusion: Both the expression of CCNL2 gene and protein were down-regulated during the process of the differentiation of P19 cells into cardiac myocytes, suggesting a possible role for this cyclin in their differentiation．

Abstract:Understanding the allocation of burden of proof which applied in the action of medical impairment compensation is entirety the key to protect the victim’s interest. The law of China applys different distribution rules for burden of proof such as inversion of the proof responsibility,high probability,bewisvereitelung to assure an objective and fair adjudication in the impairment compensation.

Abstract:Objective: To investigate the relationship between adiponectin genotype polymorphisms and obstructive sleep apnea hypopnea syndrome (OSAHS). Methods: Using the TaqMan polymerase chain reaction(PCR) method, the single nucleotide polymorphisms(SNP) at positions 45 and 276 in the adiponectin gene were determined in Chinese of the Han nationality in the Nanjing district. The OSAHS group consisted of 78 patients, and the control group contained 40 subjects. The association of adiponectin genotype polymorphisms at positions 45 and 276 with obstructive sleep apnea hypopnea syndrome was analyzed. Results: No evidence of a direct association was found between OSAHS and adiponectin genotype SNP at positions 45 and 276(P > 0.05). However, compared with those OSAHS patients having G/T+T/T genotype at position 276, the OSAHS patients with the G/G genotype showed a greater neck circumference(NC), a prolonged duration of the longest apnea event, and elevated levels of blood cholesterol and low-density lipoprotein cholesterol(P < 0.05). Conclusion: No direct association was detected between OSAHS and adiponectin genotype distribution at positions 45 and 276 in Chinese of Han nationality in the Nanjing district. However, OSAHS patients with the adiponectin G/G genotype at position 276 had a larger NC and the longest apnea event compared to those having the adiponectin SNP276 G/T +T/T genotype. This may have an indirect influence on the development of OSAHS,

Abstract:Objective: To investigate the stimulatory effect of epinephrine(Epi) and the antagonistic effect of Chrysanthemum indicum Linné extracts (CILE) on Epi-induced growth of human hepatocellular carcinoma(HCC) cells. Methods: The stimulatory effect of Epi and inhibitory effect of CILE on the growth of HepG2 and MHCC97H cells were investigated using a proliferation assay in correlation with βadrenergic receptor(β2-AR) blockade, a MEK1/MEK2 inhibitor, and assessment of MAPK/ERK1/2 intracellular activity. Results: Epi transiently activated MAPK/ERK1/2 in HepG2 and MHCC97H cells, resulting in a burst of growth. The effect of Epi was significantly attenuated by ICI 118551 and U0126. CILE exhibited a dose-dependent attenuation of the stimulatory effect of Epi on the growth of both cell lines and inhibited the Epi-induced activation of MAPK/ERK1/2. Conclusion: Epi, mimicking a mitogen, stimulated the growth of HepG2 and MHCC97H cells, and CILE was effective in attenuating this effect of Epi on tumor cells by inhibiting the β2-AR-mediated activation of MAPK/ERK1/2.

Abstract:Medical resource itself is an urban public resource. Based on the economic analysis of medical resources allocation,the article mainly discussed the approach of optimal allocation. It presented to reform the current medical resource allocation principle with the balance of rigidity and flexibility in the rapid urban development. Combining with the planning of Shenzhen Longgang district,the authors first summarized the major characteristics and influence factors of medical resource allocation,then searched for the approach of optimal allocation under the two level patterns of “city-city community”,finally put forward some relative advices.

Abstract:Transient apical ballooning syndrome(Tako-Tsubo syndrome or ampulla cardiomyopathy) occurs predominantly in women over 60 years of age with a history of recent physical or psychological stress. We present a case of a male patient with reversible transient apical ballooning syndrome with significant coronary lesions and other ECG changes that did not explain the clinical symptoms.

Abstract:Due to the medical education system,economic system and the other reasons,the level of humanities is lower in the medical practice,which urgently needs the support of medical humanities education . Medical humanities education is the inherent requirements of the essential attribute of medicine and the essential condition for achieving the purpose of medical. Medical humanities literacy should be internalized through medical humanities education.

Abstract:The article,starting from the reciprocally interactional relationship between medical humanities and physicians’ professionalism,aimed to analyze the current status of medical humanities which were neglected increasingly in the contemporary medical process as well as its significance in the construction of physicians’ professionalism. Then the authers put forward the proposal of remodeling the physicians’ professionalism through the various aspects including beliefs and quality of medical humanities,hospital humane environment,self-regulation,incentive and restraint mechanisms and humane practices,etc.

Abstract:As a relatively independent higher education system,education of medical students highly specialized courses in the targeted teaching and placed too much emphasis on science,but ignored the diversified interests and neglected humanities education at the same time, especially lacked the aesthetic quality of medical students education. From the view of applification of the high-quality medical personnel training, the article explored the methods of strengthering the aesthetic quality education of medical students.

Abstract:The teaching of epidemiology, respectively, in the application of PBL teaching mode and the traditional teaching mode of teaching, and to quantify the comparative teaching effectiveness. The results showed that:PBL teaching mode is better than the traditional teaching model on the play the students motivation to learn and cultivate student’s comprehensive ability and improve the effect of classroom teaching,and there are significant advantages. So the PBL teaching model is worth promoting.

Abstract:For the discipline construction needs,according to the head,facial,chest,abdomen,pelvis,musculoskeletal system, the teachers were divided into six learning groups,which headed by a tutor. Each group was from high,medium and low years of radiologist and was responsible for different parts of the system of research and teaching. The interns were divided into six groups,each group of 2~4 students. Each tutor was responsible for a team,not only to guide their learning,but also their thinking and life in period. The implementation of tutorial system should be “individualized” education. So that interns could acquire more professional knowledge and the latest academic medical imaging developments, learn the attitude of rigorous scholarship,a good ideological and moral from the excellent teachers. The tutorial system management mode of the medical imaging students in internship period should be a kind of humanized management mode so that the medical imaging students could achieve better internship results.

Abstract:Objective:To make the current curriculum provision of Nanjing Medical University Continuing Education College(NJMU-CEC)adapt to the development trend of modern medical education,and satisfy the requirements of the vast majority of trainees. Methods:The results of the questionnaires collected from the adult students in grade 2006,2007and 2008 of NJMU-CEC were analyzed and their opinions,suggestions and requirements from subjects concerning the current curriculum provision were found out. At the same time the curriculum provision for adult clinical medicine was compared specialty with that of other universities in and out of Jiangsu Province to identify the inadequacy of the current curriculum. Results:With the development of the medical reform,and rejuvenation and localization of student resoure,the current curriculum has been unable to adapt to the continuous development of trainees. Conclusion:The adjustment of the curriculum provision is to refine training objectives,strengthen the practical courses and the humanities courses,and focus on the overall quality of training.

Abstract:Basic surgical skill education plays a vital role in training surgical technique of medical students,and also serves as a bridge between surgical textbook and clinical practice. The article adopted a new educational system,which consisted of basic experiments as main body,comprehensive,advanced and innovative experiments as complements,to enhancing practice-based instruction. The authors also added “teaching process record” for teachers and “learning process record” for students,and carried out a control study,which used a method of instruction,demonstration,guidance,assessment and Objective Structured Clinical Examination(OSCE),among students of all the majors in Grade 2006. A satisfied result was attained at last. So it concluded that the method we used could make a great improvement to medical education,not only for surgical training,but also benefit other clinical techniques learning.

Abstract:As a decisive factor of the teaching quality and academic level of university, teaching staff is the major and the most important resource of university. Considering the actuality of the lack of college teachers, the necessity, particularity and relevant resolving strategy of the optimal allocation of teacher resource in universities were discussed in the article.

Abstract:The relief system for poor students supported by the nation,schools and society is getting perfect with a gradual expansion of aid. The national student loans under the new mechanism ensures “loans could and should be issued”,so there should be zero arrears theoretically in college. However,the colleges are facing serious arrears problem due to multi-faceted reasons,which require great attention,intensive education,positive guidance and the establishment of an effective system. A data model should be established to identify students maliciously in arrears so as to take countermeasures to solve student arrears problems.

Abstract:Objective:To understand and improve the quality of reconstruction personnel’s life,and provide a theoretical basis. Methods:112 staff of Jiangsu and 100 local residents were investigated into questionnaine of quality of life with the World Health Organization Quality of Life Scale Short(WHOQOL-BREF)from Feb to May in 2009. Results:The scores of aidworkers were higher then the local residents in the field of social relations and envioronment (P < 0.01)and lower in the subjective feelings of their own health status(P < 0.01) Conclusion:It is necessary to strengthen the protection of the reconstruction personnel’s health condition.

Abstract:Objective:To investigate the research status and the development trend of AIDS since it was discovered. Methods:The method of subject-word retrieval was used to select the articles about AIDS from two databases,PubMed and CBMWeb. The bibliometric analysis was performed. Results:The research of AIDS in our country generally kept pace with the international status. It mainly focused on the basic medicine,clinical medicine and public sanitation areas while the researchs in psychology and sociology were relatively weak. Conclusion:The research on AIDS in our country must follow the international trend and enhance the research on therapy especially on the field of prevention and control. On the other hand,the cooperation among medical personnel,psychologists and sociologists should be helpful.

Abstract:With the development of science and technology and economic globalization,the demands of internationalization to the scientific and technical journals increase. The article analyzed the changes of ratio of international papers from 2006 to 2008 of the biomedical journals(English version)in China,combined with other literature measure index to investigate the internationalizational level and problems faced by biomedical journals in English published in China

Abstract:Objective:To study the effects of a new ATP-sensitive potassium channel opener, iptakalim(IPT), on the mRNA and protein expression of vascular endothelial growth factor(VEGF) in the pulmonary artery of rats with hypoxic pulmonary hypertension (HPH). Methods:Thirty-six Sprague-Dawley(SD) male rats were randomly divided into three equal groups:the control group and hypoxic group were given 5 ml/(kg·d) 0.9% sodium chloride,ig; the treated group were given 1.5 mg/(kg·d)] IPT,ig. excepting the first group, the other two groups were put into hypoxic and normobaric chamber[(10.0 ± 0.5)%O2, 8 h/day and 6 day/week] to establish a chronic hypoxic model. After four weeks, the mean pulmonary arterial pressure(mPAP), right ventricle/left ventricle and septum[RV/(LV+S)] were measured. RT-PCR and Western-blot were performed to analyze the mRNA and protein level of VEGF in the main pulmonary artery. Result:①The levels of mPAP and RV/(LV+S) were significantly higher in the hypoxic group than those in the control group(P < 0.01). Iptakalim 1.5 mg/(kg·d) decreased the levels of mPAP and RV/(LV+S)(P < 0.01).②The mRNA and protein levels of VEGF in the hypoxic group were significantly higher than those in the control group(P < 0.01). The trented group significantly decreased VEGF mRNA and protein expression compared with those in the hypoxic group(P < 0.01). There were no differences in VEGF mRNA and protein levels between the control group and the treated group(P > 0.05). Conclusion:IPT 1.5 mg/(kg·d) remarkably inhibited the levels of VEGF mRNA and protein expression compared with the hypoxic group.Iptakalim is a promising candidate for the treatment of hypoxic pulmonary hypertension.

Abstract:Objective:To investigate the effect of movement training on the motor function repair of rats with a spinal cord contusion, and to simultaneously observe BDNF, Slit2 and GFAP expression of rats. Methods:Seventy male adult Sprague Dawley rats were randomly divided into 10 training groups and 4 control groups,each group with 5 rats. Moderate spinal cord contusive injuries were produced by using modified Allen’s impactor method to all experimental rats. After injury,rats in training groups were exposed to the treadmill locomotion test for 1 to 4 weeks. The behavioral scores were recorded and the expression of BDNF, Slit2 and GFAP in spinal cord were detected immunohistochemistry at different time point(14, 21, 28, 35 days after concusion). Results:Compared with the control groups, the behavioral scores improved significantly after 1 to 4 weeks exercise(P < 0.05),and a significant increase of BDNF,Slit2 in the spinal also were observed in exercise training groups(P < 0.05). The expression of GFAP delayed reached the peak level. Conclusion:The results indicate that rehabilitation training may play an important role in nerve functional recovery and the mechanism maybe promote the expression of BDNF and Slit2, delay hyperplasia of glial cell and weaken the inhibitory factor of neuron recovery.

Abstract:Objective:To establish a method of rapidly detecting Mycobacterium tuberculosis resistance to streptomycin and ethamtubol by gene-chip. Methods:To detect the SM and EMB resistance of 160 clinical isolates of M.tuberculosis by Gene Chip, and compare them with the results of the BACTEC960 method. Then validate the concordance and discrepancy result of the two methods by DNA Sequencing method. Results:Of 160 strains of M. tuberculosis clinical isolates, 111 strains were SM-susceptible and 49 strains were SM-resistible with Gene Chip while 100 strains were SM-susceptible and 60 strains were SM-resistible with BACTEC 960 method; 142 strains were EMB-susceptible and 18 strains were EMB-resistible with Gene Chip while 130 strains were SM-susceptible and 30 strains were EMB-resistible with BACTEC 960 method. According to the result amended by the BACTEC 960 method, the sensitivity, specialty and veracity were 80.0%, 99.0%, 91.9% and 60.0%, 100% and 92.5% respectively. The most frequent mutant sites are rpsL 43, 88 aag→agg in relation to SM-resistible and embB 306 atg→gtg in relation to EMB-resistible. Conclusion:Gene Chip method could detect the SM and EMB resistance of MTB easily and rapidly. At the same time it showed higher specialty and sensitivity, and could be used for clinical detection of SM-resistant and EMB-resistant strains.

Abstract:Objective:To study the aberrant methylation in putative promoter region of MicroRNA mir219 and the methylation correlated expression down-regulation in HCC(hepatocellular carcinoma), and explore the potential role of mir219 in HCC. Methods:In HCC tumor sample and cell line, the methylation status of putative promoter region of mir219 by pyrosequence and TA sequencing were detected; and the expression detected by Taqman real time RT-PCR. Results:There were aberrant methylation in the CpG Island 1kb upstream and stem-loop sequence of mir219 in HCC, and the mir219 transcription was also significant down-regulated in HCC cell line. Conclusion:The 1094 bp upstream and stem-loop sequence was putative promoter region of mir219, which was aberrantly methylated in HCC and transcription down-regulated in HCC. It indicated its potential role in HCC development.

Abstract:Objective:To explore the effects of Ambrolic acid(AmbA) on proliferation and apoptosis of human gastric cancer SGC-7901 cell line. Methods:MTT assay was used to observe the anti-proliferation effect of AmbA on SGC-7901 cells with morphological changes observed under optical microscopy at the same time. The apoptosis rates and cell cycle status of SGC-7901 cells treated by AmbA were determined by flow cytometry(FCM). Results:AmbA inhibited the proliferation of SGC-7901 cells in a dose and time-dependent manner. FCM analysis showed that cells were arrested in S phase and induced apoptosis dose-dependently. Conclusion: AmbA has significant antitumor activity in vitro. The potential mechanism of anti-proliferation effect caused by AmbA may be cell-cycle arrest in S phase and cell apoptosis.

Abstract:Objective:It is an interesting challenge to study green fluorescence protein(GFP) labled cells in osseous stuff(including bone bioscaffolds and nature bone), for osseous stuff is very hard and with poor lucency. To solve this problem, the authors tried to establish a semi-solid decalcification system. Methods:①Primary Mesenchyma stem cells were separated from rabbit bone marrow, modified with recombined lentivirus with GFP gene(BMSC-GFP). ②BMSC-GFP were inoculated in bone bio-scaffold of β-tricalcium phosphate(β-TCP); bio-scaffold with cells were fixed with 4% paraformaldehyde phosphate buffered saline(PBS). ③Different agents for decalcification were tested, after that agents selected were added into semi-solid system to find proper combination. ④Frozen sections were made after decalcification in semi-solid system for 1~4w, paraffin sections and plastic embedding sections were used as controls. Results:①GFP+ cells can be found growing on internal side of the holes of bio-scaffold by fluorescence invert microscope. ②BMSC-GFP cells remain at the site where they grow during decalcification in semi-solid system, discovered by fluorescence invert microscope. ③Comparing with plastic embedding sections, semi-solid system and frozen sections can keep the fluorescence and original modality, however, plastic embedding section can only be applied when scaffold is very small, while paraffin sections do not work to scaffold. Conclusion:With the own-made semi-solid decalcification system, GFP+ cells growing inside bone bio-scaffolds can keep fluorescence, modality in situ. It will be great help to the study of mechanism in many fields such as bone and cartilage tissue engineering, bone marrow transplantation and so on.

Abstract:Objective:To establish modles of rat ischemia preconditioning(I/P) and ischemia reperfusion(I/R) and to study the changes of I kappB kinaseβ(IKKβ) and Neutrophil Gelatinase-Associated Lipocalin(NGAL). Method:Thirty male Sprague-Dawley rats were divided into 3 groups randomly after right kidney nephrectomy. Group A was sham-operated control. Group B rats were 45-minutes ischemic in the left renal artery while Group C rats were pretreatmented with 3 cycles of 2-minutes ischemia and 5-minutes reperfusion. All the rats were sacrificed at 24 h after reperfusion. We harvested kidneys and serum to do further analysis, including histological and functional parameters,expressions of NGAL and IKKβ in renal tissues. Results:Compared with rats suffered from ischemia-reperfusion injury, pretreatmented rats had a significant decrease in serum creatinine level and tubulointerstitial injury scores. In addition, expressions of IKKβ and NGAL at renal tubule were attenuated significantly. Moreover, our study showed IKKβ and NGAL were in positive correlation. Conclusion:IKKβ plays roles in renal ischemia-reperfusion injury ,give rise to the generation of NGAL. IKKβ inhibition in ischemia preconditioning attenuates renal injury and the espression of NGAL following acute ischemia-reperfusion injury

Abstract:Objective:To explore the differences in protein with animal model of myocardial ischemia. Methods:Healthy pigs were used in the experiment. After thoracotomy and pericardiectomy,ameroid constrictor was implanted around the left anterior descending artery. After operation,observation was taken continuously. Then the chronic myocardial ischemia was evaluated by coronary arteriography and electrocardiogram. It is proved that 28 days after operation is the point to stable stage of myocardial ischemia with the reference to WHO diagnostic criteria. The plasma samples of model group and control group were extracted from the the superior vena cava and examined by the fluorescence differential in-gel electrophoresis(2D-DIGE) technique. Protein spots detected differential with statistical significance were identified by MALDI-TOF/TOF. Results:Intensity changes of 31 spots were detected with statistical significance. 17 protein spots of them down-regulated otherwise 14 up-regulated in model group. And 15 of them were identified by MALDI-TOF/TOF successfully. Several proteins down-regulated such as albumin,hemopexin,nicotinic acid receptor. While,CH4 and secrete domain of Swine IgM,mutated immunoglobulin heavy chain,myosin heavy chain,Ppfibp1,PRPS-associated protein 1,Ig gamma 4 chain constant region were up-regulated. Conclusion:It is proved that alkaline phosphatase,myosin heavy chain,Ppfibp1,PRPS-associated protein 1,hemopexin,nicotinic acid receptor are possibly the specific proteins for chronic myocardial ischemia. But the conclusion needs further validation and mechanism studies.

Abstract:Objective:To investigate the effects of chronic ethanol intake and withdrawal on the synaptic plasticity in dorsal striatum of rats. Methods:Rats were fed with ethanol for 10,20,30d and withdrawal 1,3,7d. Extracellular recording was used to test the changes of long-term potentiation(LTP) induced in slices of dorsal striatum and transmission electron microscope(TEM) was used to observe the changes of synaptic ultrastructure. Results:Chronic ethanol exposure suppressed the induction of LTP to different extents in dorsal striatum,with the strongest suppressive effects on LTP occurring in 10d ethanol treated rats compared with control group(P < 0.05).The depression was attenuated in 20d and 30d ethanol treated rats(P > 0.05) and notably,LTP was potentiated in withdrawl 7d rats(P < 0.05).In the synaptic ultrastructure of dorsomedial striatum,the length of synaptic active zone was longer in withdrawal 7d rats,while the width of synaptic cleft was narrower in 20d and 30d ethanol treated rats and the post synaptic density(PSD) was thinner in 30d ethanol treated rats than that of control(all above P < 0.05). Conclusion:Chronic ethanol treatment may result in the changes of the efficiency of synaptic transmission and the ability of synaptic connection in rat dorsal striatum.

Abstract:Objective:Smac is an established pro-apoptosis protein, which moderates the caspase inbitibion of IAPs. This study will clone humanβ-Smac gene and then investigate whether ectopic overexpression of it would enhance Cisplatin-induced apoptosis on gastric cancer cell line SGC7901. Methods:A pair of PCR primers forβ-Smac gene with lower primer containing a sequence of Flag was designed according to the sequence registered in GeneBank..β-Smac fragements amplificated from SGC7901 genome were cloned into plasmid pcDNA3.1 to construct the desired β-Smac transfectants. The discrepancy inβ-Smac mRNA expression between cells transfected withβ-Smac transfectants or control vectors was investigated by RT-PCR, while the ectopic overexpression of β-Smac protein in cells was detected by western blot. After transfection with either β-Smac transfectants or control vectors for 48h, cells were incubated for another 24 h in the absence or presence of Cisplatin at different concentrations. Then apoptosis was determined by flow cytometry. Results:Nucleotide sequence analysis indicated that the cloned β-Smac sequence was 100% homologous withβ-Smac gene registered in GenBank. Compared with cells transfected with control vectors, the β-Smac mRNA expression was significantly increased and ectopic overexpression of β-Smac protein was only detected in cells transfected with β-Smac transfectants. While the difference of apoptotic activity between cells incubated in 2 μg/ml Cisplatin with and without ectopicβ-Smac overexpression is statistically significant, there are no statistical differences regarding the other three groups. Conclusion:β-Smac transfectant was successfully constructed and ectopically overexpressed in SGC7901. However, the effec tis conditional and probably three is no effect of ectopicβ-Smac overexpression in enhancing Cisplatin-induced apoptosis in SGC7901.

Abstract:Objective:To investigate the effect of Resveratrol(Res)on the expression of high glucose-activated transforming growth factor-β1(TGF-β1) in rat renal mesangial cells. Methods:①Cultured rat renal mesangial cells were exposed to D-glucose at 30 mmol/L for periods of 24, 48, 72 h, respectively. Normal glucose(5.6 mmol/L) served as control. ②Cultured rat renal mesangial cells were divided into four groups:normal glucose(5.6 mmol/L), high glucose(30 mmol/L), normal glucose plus Resveratrol (20 μmol/L), high glucose plus Resveratrol at 5, 10, 15, 20 μmol/L, respectively for periods of 48 h. The mRNA levels of TGF-β1 were measured by RT-PCR and the protein levels of TGF-β1 were detected by Western Blotting. Results:As compared to normal cells, exposure of rat renal mesangial cells to D-glucose at concentrations of 30 nmol/L caused a time-dependent increase in the mRNA and protein levels of TGF-β1 and reached the highest level at approximately 48 h. The addition of Resveratrol significantly attenuated high glucose-enhanced mRNA and protein levels of TGFβ1(P < 0.05 or P < 0.01) in a dose-dependent manner. No notable difference at the mRNA and protein levels of TGF-β1 was found between Res and NC group(P > 0.05). Conclusion:High glucose contributes to an increasing of TGF-β1 expression at both the mRNA and protein levels in rat renal mesangial cells. Resveratrol can significantly reverse high glucose up-regulated expression of TGF-β1 in the rat renal mesangial cells and may play a role in the treatment of diabetic nephropathy.

Abstract:Objective:To investigate the effect of Shenfu injection(SFI) on the expression of Interleukin-18(IL-18) and Interleukin-1β(IL-1β) in neonatal rats with hypoxic - ischemic brain damage(HIBD), and clarify the protection of SFI for HI brain damage at the molecular level. Methods:Seven-day-old Sprague-Dawley rats were randomly assigned to sham operation group(S), normal sodium control group(C) and Shenfu injection treatment group(SF), and Models of postnatal 7-day Sprague Dawley(SD) rats with HIBD were established by the Rice method. They were further divided into five subgroups(n = 8 per subgroup) according to the time points 3 h,12 h,24 h,6d and 14 d. In each subgroup, rats were used to examine the level of IL-18,IL-1β in brain homogenates by ELISA. Results:The level of IL-1β was significantly higher in group C and group SF higher than that in group S at the time point 12 h, 24 h, 6 d(P < 0.01); The levels of IL-18 in group C and group SF were significantly higher than that of group S at the time point 24 h, 6 d, 14 d(P < 0.01); The expression of IL-1β in group C peaked at 12 h after HI insult and decreased after this, There was no difference in group S and group C at 14 d(P > 0.05); The expression of IL-18 in group C began to increase at 24 h after HI insult(P < 0.01), peaked at 6 d after HI insult and decreased after this. But IL-18 was still higher than group S(P < 0.01) at 14 d;the level of IL-1β in brain homogenates after HI insult in group SF were lower than group C at 12 h,24 h,6 d(P < 0.01); the level of IL-18 in brain homogenates after HI insult in group SF were lower than group C at every observed spot after 12 h(P < 0.01). Conclusion:The level of IL-18 and IL-1β in cerebral cortex had increased after HI insult in neonatal rats and SFI can decrease the level of IL-18 and IL-1β in cerebral cortex. SFI has a protedive function in neonatal rats after HI insult.

Abstract:Objective:To explore the effects and possible mechanism of recombinant human endothelin-1(ET-1) on cyclooxygenase-2(COX-2) expression in PC3 cells. Methods:PC3 cells were stimulated with ET-1(0.1-1-10-100 nmol/l) for 24 h; then BQ-123(ETAR antagonist,1 μmol/L) and BQ788(ETBR antagonist, 1 μmol/L) were used to treat PC3 cells in combination with ET-1(100 nmol/l) for 24 h. COX-2 expression in the cells was detected by RT-PCR and Western blot assay. Results:The COX-2 expression in the cells was significantly increased after exposove to ET-1(0.1-100 nmol/l) for 24 h. BQ-123 prevented the expression of COX-2(P < 0.05) while BQ-788 did not. Conclusion:ET-1 could induce the up-regulation of COX-2 in PC3 cells. ETAR might be involved during the course. Agents able to specifically interfere with COX-2 and ETAR might lead to effective treatment in the future.

Abstract:Objective:To study the effects of Kuikeling(KKL) on IL-8 levels in the serum of rats with ulcerative colitis and to research them mechanism for repairing these ulcers. Methods:Ulcerative colitis model was established by TNBS method. Total of 72 rats were randomly divided into 6 groups:normal group,model group,KKL large, medium,and little dose groups,SASP group. Colon mucosal inflammation and damage were assessed by mucosal lesion area and histological damage scores. Levels of serum interleukin-8(IL-8) were detected by ELISA. Results:Compared with the model group,the Mucosal lesionarea and histological damage scores in treatment groups were significantly improved, and the serum levels of IL-8 in the of treatment group were significantly decreased(P < 0.01). Conclusion:The present study proved that KKL has therapeutic effect on UC,it can down-regulate the serum levels of IL-8.

Abstract:Objective:To investigate the expression of vimentin protein in renal cell carcinoma and try to clarify its association with the clinicopathologic features and clinical outcome. Methods:RCC samples were obtained from 103 patients treated with radical nephrectomy. The mean follow-up period was 51.2 months. Expression of vimentin was tested by Western blot and compared with the clinicopathologic features such as tumor size, stage, grade, metastasis and survival. Results:Vimentin expression was up-regulated significantly in RCC compared with tumor-free kidney, associated with tumor size(P = 0.002), presence of metastasis(P < 0.001), high pT stage(P < 0.001), high-grade(P < 0.001) and low survival(95.8% vs 72.7%,P = 0.002). Univariate analysis revealed the significance of vimentin expression in renal cell carcinoma(P < 0.05). Conclusion:The results of this study suggest that overexpression of vimentin is associated with progression and recurrence of renal cell carcinoma. Thus, vimentin is a useful instrument for this condition.

Abstract:Objective:To isolate,culture and identify SD rat pancreas adult stem cells used the common isolation method with stem cells at present;establish a basis for further studies of pancreas cancer stem cells. Methods:We had chosen the normal SD rat pancreas digesting with collagen enzyme P. Use low serum and no glucose culture medium to purify pancreas cells. Western blot,RT-PCR and ICH to identify nestin in pancreas adult stem cells. We also proved that there was ABCG2(ATP-binding cassette superfamily G member 2)in the cells. Results:Pancreas adult stem cells and the cells expressed nestin were isolated successfully and validated by western blot,RT-PCR and ICH. Conclusion:We were successful to isolate nestin positive stem cells from SD rat pancreas used collagen enzyme P digesting and low serum,no glucose culture medium purifying.

Abstract:Objective:To study the effects of β2AdR-blockers on the differentiation and proliferation of osteoblasts deprived from rats stimulated by estrogen. Methods:Put β2AdR-blockers at different concentrations into the second filter generation osteoblasts medium containing estrogen, after culturing 3-6-9-12 days, examining the alkaline phosphatase level of the osteoblasts by PNPP method and the content of the osteocalcin besides the growth rate of the osteoblasts by RI. Results:The β2AdR-blocker increases the alkaline phosphatase(ALP) level and the level of growth rate of osteoblasts significantly,especially at the concentrations of 10-5 and 10-6 mol/L. but doesn’t increase the content of osteocalcin. Conclusion:The β2AdR-blocker can enhance the dalivery and the proliferation of osteoblasts deprived from rats stimulated by estrogen significantly.

Abstract:Objective:To investigate the role of the expression of 4E-BP1,p70S6K in RAD001 inhibition on gastric carcinoma cell lines MKN-28, N-87 and to investigate the possible mechanism. Methods:Gastric carcinoma cell lines were cultured in RPMI1640 with 10% fetal calf serum and treated by RAD001(20 nmol/L), then the cell coulter, flow cytometry, western-blot array were used to detect the growth, cell cycle and 4E-BP1,p70S6K protein expressions of the tumor cells. Results:After being treated by RAD001, the gastric carcinoma cells were inhibited in a time-dependent manner. And the percentage of Go phase was increased in MKN-28,N-87 cell lines. Meanwhile, the expressions of 4E-BP1, p70S6K, p-4E-BP1, p-p70S6K are decreased. Conclusion:The results suggested that RAD001 can inhibit the growth of gastric carcinoma cell by the adjustment of cell cycle via inhibition of mTOR, and phosphorylation of p70 S6K and 4E-BP1.

Abstract:Objective:To investigate the expression of ITGA6 and CTHRC1 protein in esophageal squamous cell carcinoma and evaluate their relationship with clinicopathological features. Methods:Immunohistochemical SP method was used to detect the expression of ITGA6 and CTHRC1 protein in 46 cases with esophageal squamous cell carcinoma and 10 cases with normal esophagus tissue. Results:①The higher expression of ITGA6 and CTHRC1 was observed in esophageal squamous cell carcinoma,the expression rates were 71.7%(33/46)and 56.5%(26/46),the negative expression was detected in 10 cases with normal esophagus tissue.②There was significantly higher expression in the cases with metastasis than these with non-metastasis(P < 0.05).③The expression of ITGA6 and CTHRC1 was not significantly related with age-sex-size of tumer and pathological type(P > 0.05). Conclusion:ITGA6 and CTHRC1 are involved in the invasion and metastasis of esophageal squamous cell carcinoma and may be new therapy targets.

Abstract:Objective:To investigate the left ventricular rotation and twist in patients with ventricular septal defect(VSD)after transcatheter closure of VSD. Methods:Using speckle tracking imaging(STI),the roation of 6 segments of basal and apical short-axises and LV twist were measured repeatedly in 18 patients with VSD before surgery,in 3rd day,1st month,3rd month after surgery.The global roation of the 6 segments in basal and apical short-axises and LV twist versus time profile were drawn,and the peak rotation of basal and apical short-axises and the peak twist of LV were caculated. LVEDd and LVEDV were caculated. LV ejection fraction(EF)was measured by biplane Simpson method. Results:Compared to before closure of VSD,the peak roation of basal and apical short-axises and peak twist of LV twist in 3rd day,1st month,3rd month after surgery were lower(P < 0.05),and the most was in 1st month after surgery. LVEDd and LVEDV were also lower in 3rd day,1st month,3rd month after surgery(P < 0.05),but the greater were in 3rd day after surgery .There were no significant differences between four groups in LVEF. The peak rotation of basal and apical short-axises and the peak twist of LV had a significant correlation with LVEF. Conclusion:After transcatheter closure of VSD,LV rotation,twist,LVEDd and LVEDV were decreased,and LV roation and twist had a significant correlation with LVEF,which may be a new index predicting LV systolic function.

Abstract:Objective:To investigate the cell-free DNA amount and DNA integrity in benign and malignant effusions. Methods:The samples of twenty-eight benign effusions and thirty-eight malignant effusions were collected and analyzed for the DNA amount and integrity by the real-time ALU-qPCR(115 bp,247 bp). Results:The content of ALU247 fragment is statistically different between two groups(P < 0.05),while there is no statistical difference between the two groups in the content of ALU115. When the DNA integrity was compared, the malignant samples had a relative higher DNA integrity(P < 0.05). AUC of ROC for DNA integrity was 0.716(P < 0.01). Conclusion:Malignant effusion samples had a significantly higher amount of long DNA and DNA integrity, and DNA integrity may be a potential biomarker to distinguish malignant effusions from benign effusions.

Abstract:Objective:To observe the effects of commanding blood pressure on prognostic condition of cerebral hemorrhage and to explore how to do. Methods:82 patients with cerebral hemorrhage(the basal regions) were divided into two groups randomly:one group(the treated group, n = 41) received a standardized command of blood pressure which included both a individual-based value range in blood pressure and a standardized treatment based on staging, drug selection, drug combination and overall target getting, and the control group(n = 41) received a traditional commanding of blood pressure. Two groups were scored with both The Chinese Stoke Clinical Scale(1995,CCSS) and Scandinavian Stroke Scale(SSS) on the day after stoke, 7days, 14days and 3months each following. The scores were statistically analyzed. Results:①The death ratio(within 3 months) in the treated group was lower than that in the control group(P < 0.01). ②The scores in the treated group were more energetic in maintain the neurons function than in the control group(CCSS,2w,3m,P < 0.01;SSS 2w,3m,P < 0.01). ③Compared pairedly with the group’s which had been not hospital cared still, the scores of the others in both the treatment and the control were changed effectively(P < 0.01). But not find a correlation between the scores of the groups after treatment and that before treatment(P < 0.01), which means prognostic condition maybe independent from the scores of the groups before treatment. ④By the way of regression analysis, not find a coefficient between the prognostic condition and each cases among age, sexuality and the amount of hemorrhage(r = 0.256,0.273,0.595). Conclusion:Commanding blood pressure standardized by the way of staging and principle above was feasible, and the standard commanding was beneficial to patients in acute cerebral hemorrhage.

Abstract:Objective:To investigate the clinical efficacy of Bisoprolol on the diastolic heart failure in the elderly hypertensive patients. Methods:Eishty-three elderly hypertensive patients with diastolic heart failure were studied. The treated group(n = 42) recieved Bisoprolol and common anti-hypertensive and anti-heart-failure drugs,while the control group(n = 41)were treated with the common drugs alone. Then were compared after treatments in the treated group and in the control group. Results:In the treatment group total effectiveness is 95%,while in the control group the total effectiveness is 80%. There is significant difference between the two groups. (P < 0.05).All data after treatments in the treated group such as isovolumic relaxation time(IVRT),early left ventricular filling velocity(E),velocity of left ventricular filling contributed by atrial contraction(A),E/A,E-wave deceleration time(DT),left atrial diameter(LAD),systolic blood pressure,diastolic biood pressure and heart rate(HR)were better than those before treatments(P < 0.01). The treatment group improved more signiticanthj than the control group(P < 0.01,P < 0.05). Conclusion:The Bisoprolol could Greduce blood pressure,heart rate obviously and improve the heart diastolic function.

Abstract:Objective:To investigate the turnover of hepatic tissue pathology,hepatitis B reactivation rate in chronic HBV carriers. Methods:A 5-year dynamic observation and research on 220 chronic HBV carriers in WuXi district has been taken via viewing the clinical symptoms,histology,virology and HBV immunological markers,et al. Results:There were differences in inflammation grade and fibrosis stage between HBeAg positive and anti-HBe positive group cases(Kruskal-Wallis Test,χ2 = 8.68,P = 0.0032. χ2 = 6.84,P = 0.0089),showing that the inflammation grade and fibrosis stage of the anti-HBe positive group was higher than that of the HBeAg positive group. There were no obvious differences in the inflammation grade between age < 40 years old and ≥40 years old group cases(χ2 = 0.62,P > 0.05),but significant statistical differences in the fibrosis stage(χ2 = 7.37,P < 0.01),showing the fibrosis stage of the ≥40 years old group cases was clearly higher than the < 40 years old group cases. 35 of 220 patients,accounting for 15.9%,showed hepatitis B reactivation. The hepatitis B reactivation rate of patients with obvious hepatic tissue inflammation(≥ G2)was 27%(33/122)and the patients with mild hepatic tissue inflammation(G0-G1) was 2%(2/98),showing a significant differences(χ2 = 25.41,P < 0.01) and the reactivation rate of patients with high inflammation was clearly higher than those with mild inflammation. 27 of 35 hepatitis B reactivation cases were older than 40 years,showing the significant statistical differences between the dissimilar age group cases and hepatitis B reactivation rate(χ2 = 6.72,P < 0.01),moreover there was no relation between the sex and hepatitis B reactivation rate. 56 cases received liver biopsy for the second time and 23 for the third time. We find that those whose pathology of hepatic tissue was normal kept stable for several years while abnormal ones hardly recovered or achieved light change. Conclusion:The hepatic tissue pathology for most chronic HBV carriers(55%) had significant abnormality(inflammation grade ≥ G2),and hepatitis B reactivation corresponded to the liver inflammation grade(G) and dissimilar age group.

Abstract:Objective:To find potential molecular targets for pancreatic cancer diagnosis by comparing and analyzing differential proteins in serum between patients with pancreatic cancer and healthy controls,providing evidence for the early diagnosis of pancreatic cancer. Methods:The protein in serum were separated by two-dimensional gel electrophoresis(2-DE)after the top 14 high abundance proteins were removed from serum with the ProteinExtractTM Removal kit.The significantly differentially expressed proteins were analyzed by matrix-assisted laser desorption-ionization time-of flight mass spectrometry(MALDI-TOF-MS),and some of them were further validated by western blot and immunohistochemistry detection. Results:Protein in serum of each case was successfully separated on 2D gels.Among seventy-eight differently expressed protein,fifty-four proteins were successfully identified by MS with twenty-nine proteins higher expressed in tumors and twenty-six lower expressed in patients. The overexpression of Ras-like without CAAX 1 and Rho-associated protein kinase 2 were further confirmed by western blot and mmunohistochemistry analysis. Conclusion:There were Significant discrepancies in serum i protein expression between patients with pancreatic cancer and healthy controls.And some of the differentially expressed proteins found by the proteomic approach may be potential molecular targets for pancreatic cancer diagnosis.

Abstract:Objective:To explore the causes and preventive ways of the bone cement leakages in a single balloon cross-midline expansion via unipedicular approach Kyphoplasty for osteoporotic vertebral body compression fractures from April 2003 to January 2008. Methods:Forty-three patients(27 male 16 female) of osteoporotic vertebral body compression fractures(range 65 to 92,average 67.7 years old) were treated with unilateral transpedicular puncture of PKP. The total vertebral body was 66. The bone cement leakages were analyzed of the postoperative X-ray films. The causes, dangerousness and the prevention of the leakages of bone cement were analyzes. Results:Marked or complete pain relief was achieved in all patients. Five vertebral bodies of 66 were showed bone cement leakage(incidence rate was 7.6%). All the patients were followed up for at least 6 months, there were no mal-outcomes. Conclusion:Unilateral transpedicular puncture of percutaneous kyphoplasty(PKP) is a minimally invasive, effective and safe procedure that provides pain relief and stabilization of spinal stability. Bone cement leakages are the main complications in the unilateral transpedicular puncture of Kyphoplasty,as long as choosing the suitable cases, obeying specification of the surgical procedures, the leakages of cement might be avoided.

Abstract:Objective:To estimate whether PAPP-A(Pregnancy-associated plasma protein-A) polymorphism is associated with pre-eclampsia. Methods:By means of polymerase Chain Reaction and Single-Strand Conformation Polymorphism,S1224Y(A/C)(dbSNP: rs7020782) polymorphism in the PAPP-A gene was detected in 129 women with pre-eclampsia(64 with mild pre-eclampsia and 65 with severe pre-eclampsia) and 137 normal control women with uncomplicated pregnancy. Investigators were blinded to clinical outcomes. Results:AC genotype frequency in pre-eclampsia group were higher than those in control group(44.2% VS 31.4%)(P﹤0.05); AC genotype frequency in severe pre-eclampsia group were significantly higher than those in mild pre-eclampsia group (75.4% VS 12.5%)(P﹤0.001). C allele frequency in severe pre-eclampsia group were significantly higher than those in mild pre-eclampsia group(40.8% VS 18.7%)(P﹤0.001). Conclusion:The AC genotype and C allele of S1224Y(A/C) polymorphism in the PAPP-A gene might be a risk genetic marker of pre-eclampsia. In patients with pre-eclampsia,there is the association between AC genotype and C allele of PAPP-A gene and severity of pre-eclampsia.

Abstract:Objective:To evaluate the value of elevated nuchal translucency(NT) as a screening test for chromosomal abnormalities(especially trisomy 21) in a first trimester ultrasound examination. Methods:A search in Cochrane Library, PubMed, OVID, Springer, China National Knowledge Infrastructure(CNKI) and Chinese Bio-medicine Database(CBM) was performed to identify relevant English and Chinese articles between Jan 1990 and August 2008. Criteria for inclusion were established based on validity criteria for diagnostic research. The eligible studies were collected and analyzed using Meta-DiSc 1.4. Results:Fourteen articles were included, with a total of 162992 fetuses. Eleven articles meeting inclusion criteria were analyzed for the value of screening for chromosomal abnormalities by nuchal translucency thickness above the 95th percentile for gestational age. The pooled sensitivity and specificity was 78.8%-92.1% respectively, AUC=0.891. Twelve articles meeting inclusion criteria were analyzed for the value of screening for trisomy 21 by nuchal translucency thickness above the 95th percentile for gestational age. The pooled sensitivity and specificity was 81.3%-92.6% respectively, AUC=0.883. Conclusion:The results suggest that screening for chromosomal abnormalities(especially trisomy 21) by nuchal translucency thickness above the 95th percentile for gestational age has a high sensitivity, specificity, accuracy and is suitable for prenatal screen.

Abstract:Objective:To establish mono-tube allele-specific amplification for typing multiplex SNPs simultaneously. Methods:Taking three SNPs of 71G > A,425A > T,and 758G > A in the TYR gene as example,a PCR pre-amplification was firstly carried out for producing a long target containing all SNPs of interest. Secondly,the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends,which were then ligated to a designed DNA adapter by ligase. Thirdly,an allele-specific amplification was performed by using all allele-specific primers and a universal primer in a single tube by using the adapter-ligated DNA as templates. Finally,the allele-specific amplification products were separated by agarose gel electrophoresis,and the types of the SNPs were discriminated by the length of the amplified products. Results:Three SNPs in the TYR gene were successfully typed for 30 healthy Mainland Chinese and the results were in agreement with those obtained by RFLP. Conclusion:This method with high specificity is accurate and cost-effective,and can be used for typing multiple SNPs simultaneously.

Abstract:Objective:To establish a method for the determination of trace mercury in spirulina powder by microwave digestion and cold atomic-absorption spectroscopy. Methods:After microwave digestion, mercury in Spirulina Powder was oxidated to the state of the ion of mercury, then the sample was detected by mercury analyzer. Results:A good linear relationship was obtained from the concentration of mercury range 0-5.00 -滋g/L,r = 0.9999,the detection limit was found to be 0.013 -滋g/L,and the experimentally determined average value for mercury in China standard reference material of Mussels(GBW08571) was found to be in good agreement with the certified value,recovery of methazolamide was between 90%-108% with the relative standard deviation(RSD) of 4.7%-Conclusion:The method of microwave digestion and cold atomic-absorption spectroscopyc was simple,sensitive, and suitable for determination of trace mercury in spirulina powder.

Abstract:Objective:To evaluate the influence of oxygen-ozone on normal nucleus pulposus in cones and the safety of intradiscal oxygen-ozone multipoint injection for the treatment of herniated lumbar disc. Methods:Oxygen-ozone at 40 μg/ml was injected into selected lumbar discs(3 ml) and the paraspinal space(2 ml) with 20G Chiba needle under fluoroscopy in twenty cones. Every cone was given multipoint injection in L5-6 and one point injection in L4-5 respectively. The whole cones were given one time oxygen-ozone injection and were sacrificed for pathology one week, two weeks, three weeks and four weeks respectively after the procedure, specimens in nucleus pulposus were observed macroscopically and microscopically. Results:No serious behavior abnormalities were observed in all animals. The atrophy of nucleus pulposus could be observed one week after oxygen-ozone injection due to significant reduction of water. The nucleus pulposus turned to dry up and looked like gray cheese after four weeks. There was an apparent difference on the cell count of nucleus pulposus between the selected L4-5 and L5-6 intervertebral disc. The cell counting of nucleus pulposus in the L5-6 intervertebral disk decreased obviously. Conclusion:It is suggested that percutaneous intradiscal oxygen-ozone injection at 40 μg/ml is a safe method, and can cause gradual atrophy of nucleus pulposus. The effect on atrophy of nucleus pulposus by multipoint injection model is better than by one point injection. This study provides the evidence of the feasibility and value of this procedure’s dinical application.