Abstract:Objective:To investigate the effects of ERK signaling passway on the class A scavenger receptor(SR-A)-mediated apoptosis in the endoreticulum stessed macrophages. Methods:The expression of phosphorylation of ERK1/2 and GRP78 was measured by Western blot;MTT analysis was used to detect the surviving fraction of RAW264.7 cells treated with different concentrations of PD98059;and apoptosis rates of RAW264.7 cells were observed by flow cytometry after staining of Annexin V FITC/ PI. Results:Fucoidan continuously activated p-ERK,and the expression of p-ERK kept on peak level through 1 to 4 hours,but was low in the SR-A gene knockout macrophages. Under ER stress,fucoidan-induced apoptosis rates of RAW264.7 cells were significantly decreased by PD98059. Conclusion:These results demonstrated that under ER stress,fucoidan could activate the ERK pathway through SR-A;and activation of p-ERK could contribute to the apoptosis of macrophages.

Abstract:Objective:To investigate the inhibitory effect on the orthotopic transplantation tumor model of human hepatocellular carcinomain using hydrodynamics-based transfection of recombinant retrovirus vector containg IκBα super repressor gene and its possible mechanism. Methods:The animal model of orthotopic transplantation tumor in nude mice was established with MHCC97-H and MHCC97-L cell lines. Tumor-bearing nude mice were divided into 4 groups:MHCC97-H group(control group),MHCC97-H+IκBαSR group(transfection group),MHCC97-L group(control group)and MHCC97-L+IκBαSR group(transfection group). After treatment for 7 days,plasmid pBABE-puro-IκBαSR and plasmid pBABE-puro in PBS solution were diluted according to the mouse weight. Plasmid pBABE-puro-IκBαSR and plasmid pBABE-puro were respectively transferred into transfection group and control group via the tail vein by hydrodynamic injection. The survival rate of nude mice bearing the tumor was observed. The size,weight and volume of tumor in situ and the number of metastatic tumor in liver were examined,and the inhibitory rates of tumor growth were calculated. ALT and AST in sera were observed. Immunohistochemistry was used to detect the expression of NF-κB in liver cancer tissue. IκBα was determined with western blot. Results:Plasmid pBABE-puro-IκBαSR significantly promoted the survival rate and inhibited the level of ALT and AST in transfection groups than in control groups. In transfection groups,the tumors were significantly smaller and lighter than those in control groups,and the inhibition rates were 29.3% and 33.0% respectively. In cellular nucleus of the transfection groups,the expression levels of NF-κB p65 and IκBα were higher than the control groups. Conclusion:Hydrodynamics-based transfection of plasimd pBABE-puro-IκBαSR gene to the orthotopic implant model of human hepatocellular carcinoma can inhibit the tumor. Its mechanism may be that the transfected IκBαSR gene suppress the activation of NF-κB signaling in cancer cells.

Abstract:Objective:To establish an NIH3T3 cell line that stably expresses GFP-V12Rac1(constitutively active Rac1 fused with a GFP tag). Methods:Plasmids and lentiviral vectors containing GFP-V12Rac1 and GFP were constructed. NIH3T3 was infected with lentiviral vectors,and cells stably expressing genes of interest were selected by flow cytometry. A cell spreading assay was used to confirm that exogenous GFP-V12Rac1 was of normal function;a Boyden chamber assay was used to test the motility of established cell lines. Results:Stable cell lines expressing GFP or GFP-V12Rac1 were established. GFP-V12Rac1 promoted cell spreading. Chemotaxis of established cell lines was confirmed. Conclusion:NIH3T3 cell lines stably expressing GFP-V12Rac1 were successfully established using lentiviral methods;exogenous GFP-V12Rac1 was of normal function;chemotaxis of the cell lines was confirmed. These cell lines can be used as model cells for further study of active Rac1 targeting.

Abstract:Objective:To construct the recombinant lentivirus containing HIV-1 Rev gene and detect the effect of Rev protein expression on the lytic cycle replication and latent infection of KSHV. Methods:The fragment of Rev gene from expression vector pCI-neo-Rev was cloned into the lentivirus vector pHAGE-CMV-MCS-IZsGreen,then the recombinant plasmid pLenti-Rev,packaging vector psPAX2 and envelope vector pMD2.G were cotransfected into the 293T cells. Culture media were harvested and filtered through a 0.45 μm filter to obtain the recombinant lentivirus. The viral titer was checked by observing the expression of IZsGreen protein. After infected with the recombinant lentivirus,the mRNA transcription and protein expression of Rev in 293T and BCBL-1 cells were detected by reverse transcription-PCR and Western blot,respectively. Then,Rev-Flag,Rta and vIL-6 protein from BCBL-1 infected with the recombinant lentivirus and transfected with pCI-neo-Rev were detected by Western blot. Further more,mRNA transcription of Rta in BCBL-1 transfected with pCI-neo-Rev was detected quantitatively by real-time PCR. Results:The recombinant lentivirus vector carrying Rev was constructed successfully with the viral titer of 2×107 TU/ml. BCBL-1 cells were efficiently infected by the recombinant lentivirus and Rev protein was readily expressed in these cells. The Western blot and real-time PCR results showed that Rta and vIL-6 expression were significantly upregulated by low Rev expression and vice versa. Conclusion:The lentivirus packaging system is available for BCBL-1,and Rev protein could express effectively in BCBL-1 infected with plenti-Rev. Forthermore,the Rev protein may regulate the lytic cycle replication and latent infection of KSHV in a complicated pattern.

Abstract:Objective:To explore whether aberrant methylation is a inhibition factor to transcriptional inactivation of SOX7 in cancer cell lines by investigation of the expression and methylation status of SOX7 gene in human pancreatic cancer cell lines. Methods:RT-PCR method was used to explore mRNA expression of the SOX7gene. Bisulfite sequencing PCR(BSP) and combined bisulfite restriction analysis(COBRA) were used to test promoter methylation of SOX7 gene in BXPC-3,CFPAC-1,PANC-1 and SW1990. Re-testing of these two indicators after the treatment of 5-aza-2-deoxycytidine(5-aza-dC). Results:SOX7 gene was obviously expressed in BXPC-3 and CFPAC-1 but silenced in PANC-1 and SW1990. The rate of methylation of CpG island of SOX7 in PANC-1 and SW1990 was much higher than the other two genes. After the treatment of 5-aza-dC in hypermethylation cell line PANC-1 and SW1990,the methylation rate of SOX7 gene promoter was decreased and the mRNA of SOX7 was re-expressed. Conclusion:This promoter hypemlethylation is correlated with SOX7 gene expression in pancreatic cancer cell line PANC-1 and SW1990 and plays a key role in SOX7 silencing.

Abstract:Objective:Using gene expression data,we compared the genotype expression profile of different 5-fluorouracil(5-FU) resistance phenotypes of pancreatic cancer cells after in vitro selection. The aims of our study were to identify genes showing altered expression in resistant derivatives,as well as several central genes or important pathways that were associated with 5-FU resistance in pancreatic cancer cells. Methods:The 5-FU-resistant MIAPaCa-2 derivatives were developed through exposure to increasing concentrations of 5-FU with repeated subcultures until the cells became fully resistant to 5-FU. Gene array analysis was performed using a Uniset Human 20 K I Codelink Bioarray. Data were analyzed with CodeLink System Software. The expression profiling of selected genes was confirmed by real-time PCR assays. Results:We got successively two resistant derivatives:the low-resistance phenotype MIA-FU-2.4 and the high-resistance phenotype MIA-FU-10.0. Analysis of array data showed that the resistance of 5-FU in pancreatic cancer cell was related to widespread transcriptional activation. In MIA-FU-2.4 and MIA-FU-10.0,we identified respectively 1075 and 1628 differentially expressed genes. The biological functions of these genes included cell cycle,cell adhesion,signal transduction,DNA repair and apoptosis et al. Conclusions:Our data suggest that 5-FU resistance development in pancreatic cancer cell MIAPaCa-2 involves in many aspects of the biological functions and might be mainly attributed to apoptosis,DNA repair and cell cycle mechanisms and some signal transductions or some pivotal genes.

Abstract:Objective:To express and characterize human tumor protein D52 (hTPD52) protein using the proper prokaryotic expression vector in E.coli. Methods:The hTPD52 gene was amplified by RT-PCR from MCF-7 cell line,and cloned into vector pMD18-T. After sequenced,the correct fragment of hTPD52 was inserted into three different prokaryotic expression vectors,which were then transformed into E.coli.BL21(DE3),respectively. The optimal expression condition was selected. After induced with IPTG,the recombinant protein was purified with His Trap affinity chromatography,and characterized by Dot blot and Western blot. Results:The hTPD52 with isoform 3 was amplified and sequenced correctly,and the recombinant expression plasmid was constructed successfully. SDS-PAGE analysis showed that the molecular weight of hTPD52-Trx fusion protein was about 44ku. The purified protein could be used as antigen to detect the specific anti-hTPD52 antibody by Dot blot and Western blot. Conclusion:The recombinant fusion protein of hTPD52 could be expressed with high performance in E.coli,and the antigenicity of hTPD52 protein is retained for the research in the future.

Abstract:Objective:To investigate the expression and significance of microRNA 200c (miR-200) and microRNA 141 (miR-141) in bladder transitional cell cancer. Methods:MicroRNA array was used to screen microRNA differentiated expressed between bladder cancer and normal peri-cancer tissue. The expression of miR-200c and miR-141 in bladder cancer samples and bladder cancer cells was determined using real-time quantitative reverse transcription (qRT)-PCR. Results:MiR-200c and miR-141 quantification showed higher expression of miR-200c and miR-141 in bladder cancer samples than normal peri-cancer tissue. The expression of miR-200c and miR-141 was higher in superficial baldder cancer than invasive bladder cancer;it was also higher in bladder cancer sample with higher grade than lower grade. Likewise,5637 cell,which is relative well-differentiated and less malignant,showed higher expression of miR-141 and miR-200c than T24,J82 and EJ cell,which were relative poor-differentiated and more malignant. Conclusion:MiR-200c and miR-141 may be involved in the progression of bladder cancer.

Abstract:Objective:To study the expression of GSK3β in chronic allograft nephropathy(CAN)model of rats and investigate the possible role of GSK3β in CAN. Methods:Fisher(F344) kidneys were orthotopically transplanted into Lewis rat recipients to set up chronic allograft nephropathy model in rats. Animals were sacrificed 12 weeks post-transplantation,and the grafts were harvested for histological study,and the expression of GSK3β and NF-κB p65 in renal tissue was analyzed by immunochemistry of SP method. Results:The expression levels of GSK3β and NF-κB p65 in CAN group were significantly higher than those in isograft group and control group. Conclusion:Our research suggests that GSK3β might play an important role in pathogenesis of CAN.

Abstract:Objective:To study the effects and related mechanisms of atorvastatin on functions of whisker barrel cortex in rats exposed to focal cerebral ischemia. Methods:Thirty male Sprague-Dawley rats,which had been trained to reach criteria,were randomly divided into sham-operation group,control group,low-dose group,middle-dose group and high-dose group. The focal ischemia model of whisker-barrel cortex was established by ligating 2-3 branches of right middle cerebral artery permanently. Animals of each dose and control group were administered with drug and saline 24h after the operation. The functions of whisker barrel cortex were evaluated each day after ischemia until rat reached the criteria again. Local cerebral blood flow was assessed by laser Doppler scanner and the expression of VEGF and CD34 was detected with immunohistochemistry method 14d after operation. Results:Compared with the control group,the functions of whisker barrel cortex were significantly improved in each dose group(P < 0.01,P < 0.01,P < 0.05);the local cerebral blood flow and the numbles of VEGF,CD34 positive cells were increased in animalsreceining differene-dose atorastatin than that of control group(P < 0.01,P < 0.01,P < 0.05 respectirely). Middle-dose atorvastatin was the best for function improvement of whisker barrel cortex in rats(P < 0.05,P < 0.01). Conclusion:Atovastatin improved the functions of whisker barrel cortex,which may be related to its increase of the numbles of VEGF positive cells and microvascular density and enhancement of local cerebral blood flow.

Abstract:Objective:To improve the detection of QRS complex in ECG signal with median filter,introduce extra criterions to determine the wave type and QRS boundaries. Methods:Based on the difference method,firstly noises such as 50 Hz noise and baseline drift were removed. After extracting amplitude and slope information of ECG signal,the ups and downs of the wave were obtained by adaptive thresholds. Results:Virtual detection system wes establish based on LabVIEW. The system was tested by MIT-BIH standard ECG database,and the correct rate reached 99.69%. Conclusion:Experiments show that the measurement is of high precision and lay the foundation for clinical ECG diagnosis.

Abstract:Objective:To investigate the changes of actual power of percutaneous microwave coagulation and the size of coagulation area under different operating time and power through performing coagulation on pork liver in vitro using water refrigeration cycle microwave antenna. Methods:Thirty fresh pork livers underwent thermal ablation using MTC-3 water refrigeration cycle microwave antenna with different operating times and powers,respectively. The change of powers of thermal ablation and the size of the coagulation area were measured. Results:The deviation between the actual power and objective power was increased with the increase of objective power. While the deviation between the actual power and objective power showed no relation with the operating time. The size of coagulation area was increased with the increase of operating time and objective power. It was more likely that the coagulation area looked like a ball when the objective power was 60-70 W and the operating time was 15 min. Conclusion:The actual power of thermal ablation is important to the efficacy. With the increase of objective power,the attenuation of power is increased. The coagulation area shows as a ball with an objective power of 60-70 W and an operating time of 15 min. This study might provide evidence for thermal ablation used to treat liver cancer.

Abstract:Objective:To study effects of different durations of thromboembolism on hemodynamics,pulmonary arteriography and right ventricle myocardial pathology in a rabbit model with embolization of pulmonary lobar artery. Methods:Twenty four rabbits were randomly divided into control group,mild pulmonary thromboembolism(PE) model group and severe PE model group,with 8 rabbits each. PE was induced by infusing autologous blood clots(right jugular vein). Arterial blood gas were analyzed and the plasma’s concentrations of cTnI and NT-proBNP were examined by ELISA before and 1 h,2 h,4 h,8 h after injection,and the hemodynamic monitoring was used by homemade catheter with multi-channel physiological recorder. The pathological changes of the lung and right ventricle myocardium were examined with light microscope at 12 h after injection. Results:①hemodynamic monitoring showed that RV systolic pressures(RVSP)increased and MAP declined significantly in the severe PE model group,then gradually return to normal after 4 h. There were obviously differences between the severe PE model group and control group(P < 0.01) and no differences between the mild PE model group and control group(P > 0.05);②ELISA showed that NT-proBNP in the severe PE model group increased at 2 h,peaked at 8 h compared to those in the control group and the mild PE model group(P < 0.01). cTnI in severe PE model increased at 4 h,peaked at 8 h,but no obuious changes in control group and mild PE group;③ Histopathological study showed that injury of right ventricle myocardium and lung were obvious in severe PE model group with inflammatory cell infilitration,and also show there was vacuolar degeneration of right ventricle myocardiu in severe PE model group,but was less in mild PE model group. Conclusion:The change of cTnI,NT-proBNP and hemodynamic prove that severe PE results right ventricle dysfunction. We can use cTnI,NT-proBNP as facilitate triage of PE,and should pay attention to injury of right ventricle myocardium and RV dysfunction in clinic.

Abstract:Objective:To construct GIT1-WT(full-length avian GIT1)and GIT1-Y293F lentivirus vectors and to investigate the role of 293 site in GIT1. Methods:The GIT1-WT gene was obtained by polymerase chain traction(PCR)from mouse gene bank. The PCR products were inserted into PLJM-GFP plasmid. The PLJM-GFP-GIT1-Y293F was obtained by TaKaRa MutanBEST Kit. Both PLJM-GFP-GIT1-WT and PLJM-GFP-GIT1-Y293F were evaluated by PCR and sequencing. The virus obtained from transfected 293T cells was infected into osteoblasts. The role of GIT1-Y293F in ostroblasts migration was determined by wound healing assay. Results:The PLJM-GFP-GIT1-WT and PLJM-GFP-GIT1-Y293F were constructed successfully. The results of wound healing assay showed that GIT1-Y293F lentivirus vectors significantly inhibited osteoblast migration compared with GIT1-WT. Conclusion:The functions of GIT1 dependent on the phosphorylation of 293 sites.

Abstract:Objective:To study the relationship of the level of phospho-p38MAPK (p-p38MAPK) and the number of survival neurons in hippocampal CA1 regions by ischemic preconditioning(IP) after cerebral ischemic-reperfusion(IR). Methods:The model of ischemic-preconditioning in gerbil was established. The animals were randomly allocated into SH group,ischemia in advance group(IA group),IR group and IP group. After the last ischemia,the animals of the last three groups were respectively further allocated into subgroups of 15 min,2 h,6 h,1 d and 5 d after reperfusion with 8 animals in each subgroup. The p-p38MAPK in hippocampal CA1 regions was detected by immunohistochemistry and semiquantitive analyzed using Leica Qwin image analysis system in the former 4 subgroups. The behavioral and morphologic changes were detected in last 2 subgroups. The myringa temperature of gerbils sustained at (37±0.5) ℃ from the beginning of ischemia to recovery from anesthesia. Results:There was few p-p38MAPK detected in hippocampal CA1 regions in SH group. The level of p-p38MAPK in hippocampal CA1 region in IA group was significently higher than that of SH group,but significently lower than that of IR group at 15 min,2 h,6 h and 1 d respectively. The level of p-p38MAPK in hippocampal CA1 region in IP group was significently higher than that of IA group at 15 min and 2 h respectively,but was remarkably lower than that of IR group at 15 min,2 h,6 h and 1 d respectively. The behavioral examination showed that gerbils scrawed more actively in IR group at 1 d and 5 d and in IP group at 1 d than those of SH group. Gerbils in IP group scrawed much less actively at 1 d and 5 d than those of IR group. The survival neurons in hippocampal CA1 region of IR group and IP group were less than those of SH group at 1 d and 5 d respectively. The survival neurons of hippocampal CA1 region in IP group were significently more than those of IR group at 1 d and 5 d respectively,but less than those of IA group at 5 d after reperfusion. Conclusions:The level of p-p38AMPK in hippocampal CA1 increased after 3 min IR,and it could reduced the level of p-p38MAPK after another longer time IR after 1 d. IP reduced hippocampal CA1 neurons death and attenuated behaveral changement after longer time IR.

Abstract:Objective:To investigate the effects of breviscarpin on GluR2 protein and mRNA expression in hippocampal CA1 neurons of rats following transient forebrain ischemia. Methods:Twenty-five male SD rats weighed from 250~300 g were randomly divided into 5 groups(n=5 each):groupⅠ(sham operation),groupⅡ(ischemia/reperfusion for 2 days),group Ⅲ(ischemia/ reperfusion for 4 days),group Ⅳ(breviscarpin combined with ischemia/ reperfusion for 2 days),groupⅤ(breviscarpin combined with ischemia/ reperfusion for 4 days). The rats of Ⅱ,Ⅲ,Ⅳand Ⅴgroup were subjected to transient but severe forebrain ischemia by permanently occluding the vertebral arteries and 24 hours later temporarily occluding the common carotid arteries for 10 minutes. GroupⅠ animals were treated identically,except that the carotid arteries were not occluded. The rats of Group Ⅳ and Ⅴ were injected with breviscarpin(45 mg/kg) in abdominal cavity at 30 min before ischemia. The other groups were injected with equal 0.9% NaCl saline at the corresponding time. For quantization of GluR2 protein /GluR2 mRNA expression,rats were anesthetized and decapitated and the hippocampal CA1 were removed 2 days and 4 days after cerebral ischemia. GluR2 protein expression was assessed by Western Blot. RT-PCR was performed to assess the expression of GluR2 mRNA. The density of the bands was analyzed. Signal intensities were normalized against the internal standard(Western blot by GAPDH,RT-PCR by β-actin). Results:The GluR2 protein and mRNA expressions of group Ⅱand Ⅲ were both lower than those of groupⅠ(P < 0.01). But the expressions of group Ⅳ and Ⅴwere higher than those of groupⅡand Ⅲ at the corresponding time(P < 0.01). Conclusion:Breviscarpin attenuates down-regulation of GluR2 protein and mRNA after forebrain ischemia,which may be one of the inportant reasons rescuing hippocampal CA1 neurons after forebrain ischemia by breviscarpin.

Abstract:Objective:To compare the clinical efficacy,the injury and the acute phase reaction in perioperative period of lobectomy between totally video-assisted thoracoscopic surgery(VATS)and traditional open surgery(TOS)in non-small cell lung cancer(NSCLC)patients. Methods:Forty cases of NSCLC undergone lobectomy were enrolled in this study,including 20 treated by VATS and 20 by TOS. The clinical efficacy and the concentration of plasma DNA and CRP level in perioperative period were compared and analyzed statistically. Results:The surgeries in both groups were all successful. No death and no postoperative complication occurred. There was no difference in the operation time,the groups and the numbers of the lymph node cleaned and the time and volume for chest drainage between two groups(P > 0.05). The average bleeding volume during operation,the postoperative pain and the day of out-of-bed activity and hospitalization stay after operation were significantly lower in VATS group than in TOS group(P < 0.05).The concentrations of plasma DNA and CRP level were not different between two groups before the operation(P > 0.05). The concentrations of plasma DNA and CRP level were significantly lower in VATS group than those in TOS group in postoperative 1 day,3 day and 5 day(P < 0.05). Conclusion:Compared with TOS,VATS has no diffience in clinical efficacy in perioperative period,but can significantly decrease injury severity,acute phase reaction and the pain of the patients,and has obvious advantages of minimal invasion to patients.

Abstract:Objective:To investigate the clinical results of primary closure of the common bile duct(CBD)by open and laparoscopic surgery in treatment of choledocholithotomy. Methods:A total of 86 patients with primary closure after the CBD exploration between January,2006 and August,2008 were enrolled,including 30(35%)umdergone laparoscopic choledochotomy(laparoscopic group)and 56(65%)received open choledochotomy(open group). The clinical data in the two groups were analyzed comparatively. Results:The patients of two groups were cured to discharge from hospital. No mortality and no complications such as postoperative bleeding or pulmonary infection occurred in two groups. There was no significant difference in bile duct retained stones,postoperative infection and bile leakage between two groups. On the volume of intraoperative bleeding,time of gastrointestinal recovery,abdominal drainage and postoperative stay,the laparoscopic group was much superior to the open group(P < 0.05). However,the laparoscopic group had a longer operation time and a higher hospital expense than the open group. Conclusion:Laparoscopic group has a better postoperative recovery but a longer operation time. Comparatively,the open group with wider range of indication has a lower cost of treatment and is similar with the laparoscopic group in the incidence of postoperative complications.

Abstract:Objective:To evaluate the clinical value of detection of circulating tumor cells of breast cancer by nested RT-PCR using combimation of cytokeratin 19(CK19)and small breast epithelial mucin(SBEM). Methods:The reverse transcripted products of the gradient mixture of the RNA of MCF-7 cells and peripheral mononuclear cells of healthy volunteers were detected by the optimized nested RT-PCR to evaluate its sensitivity. Peripheral blood of 10 healthy volunteers and 20 patients of benign lesions of breast were detected to evaluate its specificity,and peripheral blood of 56 patients of breast cancer were detected. Results:The optimized detecting system detected MCF-7 cell from peripheral mononuclear cells of 3 ml peripheral blood,and no positive expression of CK19 mRNA and SBEM mRNA were found in 10 healthy volunteers and 20 patients of benign lesions of breast. The expression of the two markers was related to the size of primary tumor,axillary lymph nodes status and TNM(P < 0.05),but not related to the status of ER,PR and C-erbB-2(P > 0.05). Conclusion:The optimized nested RT-PCR system achieved higher sensitivity and specificity,could detect the expression level of CK19 mRNA and SBEM mRNA effectively in the peripheral blood of breast cancer patients;CTC detection is useful for the assessment of prognosis and treatment of breast cancer patients.

Abstract:Objective:To investigate the relationship between levels of serum thyroid stimulating hormone(TSH)and blood pressure in the school-age children and adolescents. Methods:A cross-sectional survey on 880 subjects aged 7~18 in Bengbu,Anhui province was carried out with a questionnaire,blood pressure,height,and body weight measurement on the basis of random cluster sampling method. Blood samples were taken in fasting state to test TSH. FT3 and FT4 were further examined if TSH was abnormal. The relationship between different levels of serum TSH and systolic and diastolic blood pressure(SBP and DBP)was analyzed. Results:①Serum TSH and SBP,DBP were positively correlated. After adjusting for age,sex,and body mass index(BMI),the positive correlation between TSH and blood pressure was still remained and was more significant in male(correlation coefficient r=0.115 5,0.130 2,P=0.007,0.002). ②SBP and DBP in subclinical hypothyroidism group were higher than those in normal subjects,increased by 2.6 mmHg,2.2 mmHg,respectively. Conclusion:SBP and DBP were increased with the increment of TSH in normal subjects and the participants with subclinical thyroid disease. Among the school-age children and adolescents,blood pressure was higher in subclinical hypothyroidism subjects than that in normal. This study provides evidence for screening thyroid function in hypertensive children and adolescents and treatment for subclinical hypothyroidism.

Abstract:Objective:To investigate the variety of resistin,CRP,TNF-α and IL-1β in the rat of acute pancreatitis. Methods:Forty Sprague-Dawley rats were randomly divided into four groups:control group,sham operation group,AEP group and ANP group. AEP model was induced by intraperitoneal injection of cerulein,and ANP model was established by intraperitoneal injection of L-arginine while the control group didn’t receive any interference and the sham opertation group received same volume injections of 0.9% saline solution. The level of amylase,resistin,CRP,TNF-α and IL-1β were determined in blood serum by using ELISA,and pancreas/body weight ratio was evaluated and pancreatic pathology was recorded. The level of resistin was detected in pancreas by immunohistochemistry. Results:The levels of amylase,pancreas/body weight ratio,pancreatic pathology,resistin,TNF-α,IL-1β and CRP were respectively(4376.70±342.95)U/L,(8.67±1.43)g/kg,5.39±0.26,(10.21±3.34)ng/ml,(184.18±45.24)pg/ml,(194.24±44.81)pg/ml,(3585.85±157.28)ng/ml in AEP group;and were respectively (6750.2±321.81)U/L,(9.33±1.76)g/kg,7.81±0.28,(15.14±3.11)ng/ml,(349.31±94.54)pg/ml,(315.59±37.04)pg/ml,(4345.04±661.53)ng/ml in ANP group. All the indexes were significantly higher than those in the control group and the sham operation group(P < 0.01),the levels of the indexes in ANP group were higher than those in AEP group(P < 0.01 or P < 0.05). Resistin was correlated with the increase of CRP,TNF-α,IL-1β and the pathologic severitity of pancreatitis,respectively,(r=0.711,0.812,0.794,0.812,P < 0.01). The pathologic severitity of pancreatitis was correlated with the level of CRP,TNF-α,IL-1β. Conclusion:The resistin is correlated with the pathogenesis and development of acute pancreatitis,and may be useful to predict the severity and prognosis of acute pancreatitis.

Abstract:Objective:To investigate the impact of GSTT1 and GSTM1 genotypes on early treatment response and chemotherapy toxicities of childhood acute lymphoblastic leukemia(ALL). Methods:GSTT1 and GSTM1 genotypes were analyzed with PCR in 98 ALL patients. The early treatment response and chemotherapy toxicities were compared between groups with or without GSTT1 and GSTM1 genes. Results:Patients without GSTT1 gene had better early treatment response than patients with GSTT1 gene(OR=3.35,95%CI:1.05-10.73,P=0.041). The risk of poor early treatment response in patients with GSTT1 and GSTM1 double present was much higher than that in patients with GSTT1 null or GSTM1 null(OR=5.73,95%CI:1.73-18.95,P=0.004). The risk of oral mucositis,heptatoxicity and infection in patients with GSTM1 null was higher than that in patients with GSTM1 present(P < 0.05). Patients without GSTT1 and GSTM1 genes experienced more heptatoxicity and infection than patients with GSTT1 and GSTM1 genes(P < 0.05). Conclusion:GSTT1 and GSTM1 genotypes were apparently related to early treatment response and chemotherapy toxicity of patients with ALL. GSTT1 and GSTM1 genotypes might be useful in selecting appropriate chemotherapy regimens for patients with ALL.

Abstract:Objective:To investigate the levels of neuron-specific enolase(NSE)in serum and to explore neuronal damage in absence epilepsy children after seizures. Methods:Twenty two children with absence epilepsy were enrolled,according to whether the hyperventilation induced absence seizures or not. The children were divided into two subgroups:10 cases of absence seizures group/12 cases of non-seizure group. and twenty healthy children served as normal control group. The blood samples were collected within 30 minutes after seizure. An enzyme-linked immunosorbent assay(ELISA)was used to detect the serum NSE in all the children. Results:The serum levels of NSE in the absence seizures group were significantly higher than those in non-seizure group and normal control group(P < 0.01);but there was no significant difference between non- seizures group and normal control group (P > 0.05). Conclusion:The levels of serum NSE in children with absence epilepsy were markedly increased after seizures. suggesting that a certain degree of neuronal damage may result from absence seizures. Neuron-specific enolase may server as an important specificity of biochemical marker in the early detection of neuronal damage after absence seizures.