Abstract:Objective:To study the role of iNOS in small-for-size graft. Methods: A non-arterialized partial liver transplantation model was used. Rats were divided into three groups:control group,small-for-size group,Aminoguanidine (AG) group. The expression of the iNOS mRNA and protein,the level of alanine aminotransferase(ALT) in serum,as well as histology were checked after reperfusion. Results: The expression of the iNOS in AG group was lower than that of the small-for-size group,but the expression of ALT in serum sample was significantly higher than that of the small-for-size group(P < 0.05,n = 5). Histology showed necrosis was observed both in the small-for-size group and the AG group. Conclusion: AG can inhibit iNOS in small-for-size graft,but lead to the worse injury.

Abstract:Objective: To investigate the expression of metastasis associated 1(MTA1) in human hepatocellular carcinoma(HCC)and adjacent tissues,and its correlation with survival and recurrence,to consequenthy determin whether MTA1 is a suitable prognostic index for prognosis or not. Methods: The transcription and translation of MTA1 gene in HCC tissue,corresponding adjacent tissue,and normal tissue samples were determined by quantity real-time PCR and immunohistochemistry(IHC),and the correlation between MTA1 and survival and recurrence of HCC patients was analyzed by Kaplan-Meier curve followed by log-rank test. Results: The transcription and translation of MTA1 gene were significantly higher in the HCC tissues and the adjacent cirrhotic tissues compared to the normal liver tissues(P < 0.01). Expression level of MTA1 was related to survival and recurrence rates of the HCC patients. Conclusion:MTA1 was a suitable prognostic index predicting survival and recurrence after surgical resection of HCC.

Abstract:Objective:To explore the possible role of JWA in the process of adhesion,migration and invasion of human hepatocellular carcinoma(HCC). Methods:The small interfering RNA(siRNA) was used to inhibit JWA expression in HCC cell line MHCC97L,and its effects on cell adhesion,migration and invasion were investigated. Results:Depletion of JWA caused a notable increase in cell adhesion,migration and invasion potential of MHCC97L. Conclusion:This study showed that reduced JWA expression in MHCC97L was associated with tumor adhesion,migration and invasion potential,indicating that JWA may be a metastasis suppressor gene for hepatocellular carcinoma.

Abstract:Objective: To study the effect of PCSK9 on the LDL-C metabolism in response to infection and inflammation. Methods:C57B/6J male mice were injected with low dose liposaccharide (LPS) to mimic acute phase responce. Plasma lipids levels were detected. Serum amyloid A levels in peripheral circulation and purified fast protein liquid chromatography (FPLC) fractions have been assayed by ELISA. Real time PCR has been used to measure PCSK9,LDLR,HMGR and other genes mRNA level in mice liver. Results: Low dose LPS treatment induced abundant serum amyloid A secretion and marked changes in lipid and lipoprotein metabolism,including high total cholesterol and triglyceride levels. We explored the effect of LPS on PCSK9 expression,and found LPS resulted in a marked increase in hepatic PCSK9 mRNA levels (2.88-fold increase). Furthermore,we demonstrated that LPS decreased hepatic LDL receptor protein but at the same time hepatic LDL receptor mRNA levels were not decreased. Conclusion: Thus PCSK9 expression stimulated by inflammation led to increased LDL receptor degradation and decreased LDL receptors,and thereby increased serum LDL. The results suggest that proinflammatory states,such as those associated with obesity and insulin resistance,may be associated with upregulated hepatic expression of PCSK9.

Abstract:Objective:To verify the involvement of ERα and ERβ on acute and persistent pain modulation by estradiol. Methods: The involvement of both receptors on nociceptive responses was measured by tail-flick, hotplate and formalin tests in αERKO and βERKO female mice. Ovariectomies followed by estrogen replacement were performed in the groups to insure comparable sex hormone levels. Results: WT mice given E2 showed no changes in tail-flick latency and hot plate latency. There were also no significant differences between WT and αERKO or βERKO mice. Estradiol significantly reduced the overall number of flinches in PhaseⅡ(not phase Ⅰ) during the formalin nociceptive response. Using ERKO mice, we found that βERKO (not αERKO) female mice showed lower nociceptive responses compared to WT female mice in the phase Ⅱ( not phase Ⅰ) in the formalin test, but suggesting that the pain was modulated by ERβ. Conclusion: Estrogen specifically influences nociceptive responses ,and ERβ plays an important role in endogenous pain modulation systems.

Abstract:Objective: To study proliferation and biological functions of 4-1BBL reverse signals in the primary blast cells of human acute myeloid leukemia(AML). Methods: Twenty-six AML patients were enrolled. The expression of 4-1BBL protein was detected by FACS in human primary AML cells. Anti-4-1BBL mAb(1F1) was used to stimulate primary human myeloid blast cells. Cell proliferation was determined using Alamar Blue assay. The production of cytokine matrix metalloproteinase 9(MMP-9) in the culture supernatants of AML cells. was determined by a cytokine-specific ELISA. Results: FACS analysis showed that 4-1BBL molecule was constitutively expressed on AML cells. Among the 26 cases,46.15% expressed 4-1BBL molecule. Anti-4-1BBL mAb 1F1 stimulated the proliferation of 4-1BBL positive AML cells. The level of MMP-9 in the culture supernatants of AML cells treated with 1F1 and isotype IgG1 for 48 hours were(965.06 ± 229.94) pg/ml and(596.49 ± 129.28) pg/ml,respectively(P < 0.01). Conclusion: 4-1BBL molecule is constitutively expressed on human AML cells. 4-1BBL reverse signaling plays a critical role in AML cells survival and growth,and contributes to MMP-9 release.

Abstract:Objective:To investigate the effect of ketamine on the differentiation of human T helper(TH) cells and activity of T-bet and GATA3 in vitro. Methods: Ten male healthy volunteers (aged 20~45y,BMI 18~25 kg/m2) were enrolled in this study. Tweenty milliliter peripheral venous blood was collected from each volunteer and peripheral blood mononuclear cells(PBMC) were isolated and incubated in the presence of phorbol-myristate-acetate (PMA)(25 ng/ml) plus ionomycin (1 -滋g/ml) and various concentrations of ketamine(0,50,100,200 -滋mol/L). TH subsets,supernatant interferon γ (IFN-γ) and interleukin 4 (IL-4) levels,and the DNA binding activity of intracellular transcriptional factors T-bet and GATA3 were detected and analyzed after four hours culture. Results: TH1 and TH2 cell numbers,IFN-γ and IL-4 levels were significantly increased by PMA and ionomycin stimulation. Ketamine dose-dependently decreased TH1 and TH2 cell numbers,IFN-γ,and IL-4 levels,but increased the ratio of TH1/TH2 and IFN-γ/IL-4 in the presence of PMA and ionomycin. T-bet and GATA3 activities in PBMC were significantly increased by PMA and ionomycin stimulation. Ketamine dose-dependently decreased T-bet and GATA3 activity,but increased the ratio of T-bet/GATA3 activity in the presence of PMA and ionomycin. Conclusion: Ketamine can suppress TH cell differentiation and subsequent cytokines. But it increase TH1/TH2 ratio after PMA and ionomycin stimulation,which may be related to its regulation on T-bet and GATA3 activity.

Abstract:Objective: To construct recombinant prokaryotic expression vector to express the important NIDO Domain protein and further purify and identify the NIDO protein. Methods: The NIDO gene was subcloned into pMD19-T vector for DNA sequencing analysis. Plasmid extraction,purification,enzyme,connection,competent cell preparation and transformation technology were performed to construct and identify the prokaryotic expression vector pGEX-4T-1-NIDO. The protein NIDO-GST was expressed in E.coli-BL21 as a fusion protein induced by IPTG. The expression products were isolated,and the target proteins of supernatants and inclusion precipitation were detected by 12%SDS-PAGE. After denaturation and renaturation of inclusion body,the expression products were purified by GST-affinity chromatography. The purity of NIDO-GST fusion protein was identified by 12% SDS-PAGE and the Mass-Spectrographic analysis. Results: NIDO coding region was cloned into pMD19-T vector and the sequence was confirmed. Prokaryotic expression system of pGEX-4T-1-NIDO was constructed successfully,and were induced in E.coli BL21,to obtain high expression of N-terminal with GST tag sequence of the recombinant fusion protein(NIDO-GST) expression(30%). Target protein in bacterial precipitation was in the form of inclusion body mostly after Ultrasonic crack bacteria. After denaturation and renaturation of the inclusion body and further GST affinity chromatography purification,the purity of protein NIDO-GST was more than 80% and confirmed by mass spectrum identification. Conclusion: The purified fusion protein NIDO-GST was constructed successfully by the prokaryotic expression and affinity chromatography.

Abstract:Objective: To establish a culture method for primary mouse brown adipocyte precursor cells. Methods: Fibroblast-like cells were collected from C57BL/6j mice brown adipose tissue. The morphological changes of the cultured cells were observed,the intracytoplasmic lipid of the culture cells was determined using oil red O staining,and the expression of the brown adipocyte specific gene was determined by real time PCR. Results: The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation and differentiate into mature adipocytes. Oil red O staining,morphological observation,and expression of brown adipocyte specific gene UCP-1 verified these cells as brown adipocyte. Conclusion: Brown adipocyte precursor cells are present in C57BL/6j mice brown adipose tissue and possess the potential to proliferate and differentiate into mature brown adipocyte. The establishment of primary culture of mouse brown adipocyte precursor cells is important for furth studies of the function of brown adipocyte in vitro.

Abstract:Objective:To explore the effects of glutamate and the NMDA receptor on the outward K+ currents,and illustrate the mechanisms for glutamate induced neuronal damage. Methods: Neurons were collected from18-day-old embryonic rats in the present work and were divided into two groups:the control and glutamate treated group. The currents were recorded by performing the whole cell patch clamp,and subsequently the cell morphous as well as neuron apoptosis were evaluated by morphology and TUNEL assay. Results: Glutamate obviously increased the outward K+ currents and MK-801,the NMDA receptor antagonist,significantly alleviated the outward K+ currents. The morphology and TUNEL results revealed that the treatment of glutamate resulted in neuronal damage (P < 0.01) while MK-801,the blocker of K+ channel TEA,as well as high concentration of K+ solution substantially reduced the cell damage(P < 0.01). Conclusion: The outward K+ currents were involved in neuronal damage induced by glutamate,and the mechanisms need further clarified.

Abstract:Objective: To compare the difference of creep tendency and stress distribution in periodontal tissue by using of flat stainless steel rectangular arch,20°torque archwire and compound retraction arch wire in retraction of 4 upper incisors. Methods: A model was established by 3D finite element analysis,on which three kinds of archwire was loaded. An additional moment was added to the model with the compound retraction arch wire and 20° torque archwire. The stress distribution in periodontal tissue was calculated. Results: A crown lingual movement tendency was observed when the flat stainless steel rectangular arch was used. When the other two wires were used,a crown labial movement tendency was observed. However,the maximum stress of the compound retraction arch model was less than the 20°torque archwire. Conclusion: Compared with the other two kinds of archwire,the compound retraction archwire can decrease the injury to the periodontal tissue and achive a better therapeutic effect.

Abstract:Objective:To evaluate antioxidant nutrients’ protection on reproductive oxidative damage male rats induced by acrylamide(AA). Methods:Six-week-old SD male rats were randomly divided into 6 groups. The six groups were negative control group,positive exposure group,VitE group,VitC group,selenium group and VitE+selenium group,respectively. After four weeks,rats were killed to collect serum,liver,testis,epididymis,and then the contents of AA,glycidamide (GA),malonaldehyde(MDA),superoxide dismutase (SOD),glutathione-S-transferase(GSH-ST) and liver microsomal cytochrome P450 2E1 were determined. Results: Rats exposed to AA had significantly decreased weight,liver index and epididymis coefficient,and the SOD,GSH-ST levels in serum and the MDA level in testis were significant different compared with the controls. Compared with the positive exposure groups,the SOD,GSH-ST levels in serum and the MDA level in testis of the antioxidant nutrients groups were significant different. Compared with the VitE,selenium groups,the MDA levels in testis of VitE+Se group had significant differences. The activity of liver microsomal cytochrome P450 2E1 in VitE and VitC groups had a statistical difference compared with the positive exposure group. Conclusion: The antioxidant nutrients had a protective effect on male reproductive oxidative damage in rats induced by AA,and VitE+selenium protective effect of better use.

Abstract:Objective: To explore the relationship between neuronal nitrie oxide synthase (nNOS) molecule and antidepressant-like effects of fluoxetine. Methods:The antidepressant activity was assessed in forced swimming test (FST) and tail suspension test (TST) behavioral paradigm. Total immobility time was registered during the period of 5 or 6 min. ICR mice were treated with fluoxetine or pretreated with L-arginine/D-arginine,and wildtype(WT) mice and mice lacking nNOS gene[knock-out (KO)]were treated with fluoxetine,and then the immobility time in the FST and TST was analyzed. Results: In ICR mice,the immobility time in the FST and TST as reduced by fluoxetine treatment,and the antidepressant-like effect of fluoxetine in the FST and TST was prevented by the pretreatment with L-arginine,but not D-arginine. In WT mice,administrations of fluoxetine caused antidepressant-like effect in the FST,whereas in KO mice fluoxetine was ineffective. Conclusion: nNOS molecule is required by antidepressant-like effect of fluoxetine.

Abstract:Objective: To investigate the effects of hypoxia on the expression and activity of SIRT1 and its regulatory mechanisms on human immune function. Methods: Human primary peripheral blood monocytes(HPBMs) were cultured in vitro under normal oxygen and hypoxia(1% O2) conditions. The protein expression of SIRT1 was detected by Western blot. Real-time PCR was performed to examine the mRNA levels of SIRT1,NAMPT and HLA-DR-琢. Activity assay kits were used to detect the activity of SIRT1 and value of NAD+/NADH. Results: SIRT1 protein level was inhibited with peak inhibition occurring at 12 h post exposure to hypoxia. The mRNA levels of SIRT1 and NAMPT in cells cultured in hypoxia were significantly lower than that cultured in normal oxygen(P < 0.05). In addition,the enzyme activity of SIRT1 and the value of NAD+/NADH were also significantly decreased (P < 0.05). Resveratrol, which is the agonist of SIRT1,rescued the decreased expression of HLA-DR-琢 induced by hypoxia. Conclusion: The CⅡTA-dependent HLA-DR-琢 expression was decreased,and accompanied with a decrease in the expression and enzyme activity of SIRT1 in macrophages exposed to hypoxia. These results revealed that SIRT1 may play a critical role in regulating adaptive immunity.

Abstract:Objective: To observe the effects of dexamethasone and TLR4 in acute lung injury rats induced by freshwater drowning. Methods: Seventy-two rats were randomly divided into three groups:the control group,the freshwater drowning group and the dexamethasone group. A rat model of acute lung injury was induced by freshwater drowning by means of introduction freshwater into trachea of rats. At the time point of 0,0.5,2.0,4.0,8.0 and 24.0 h,the change of PaO2 was determined. At the time point of 0.5,2.0,8.0,and 24.0 h,the change of MPO,W/D and TLR4 mRNA in lung tissues,and the change of TNF-α and IL-6 in serum was determined. Results: Acute lung injury model induced by freshwater drowning was successfully reproduced in rats. Compared with the control group,PaO2 in the drowning group was decreased significantly at the time point of 0.5 h after infusion of freshwater,then elevated but maintained at a low level. The level of TNF-α and IL-6 in serum was increased at 0.5 and 2.0 h respectively. The value of W/D,MPO and TLR4 mRNA in lung tissues was enhanced at 0.5,2.0 and 8.0 h respectively. Dexamethasone alleviated lung injury induced by freshwater drowning through suppression of the expression of TLR4 mRNA and inflammatory factor. Conclusion: TLR4 may participate in acute lung injury induced by freshwater drowning,whereas,dexamethasone could suppress TLR4 mRNA and protect the lung from pulmonary freshwater drowning injury.

Abstract:Objective: To investigate the short-term prognostic value of stromal cell-derived factor-1α(SDF-1α) in patients with acute ST-segment elevation myocardial infarction (STEMI) referred to primary percutaneous coronary intervention(PCI). Methods:Serum levels of high sensitivity C-reactive protein (hs-CRP),SDF-1α were measured at baseline,24 and 48 hours after PCI or coronary angiography(CAG) in 78 patients with STEMI,underwent primary PCI,60 patients with stable angina pectoris(SAP) for PCI and 60 healthy adults confirmed by CAG. Major adverse cardiac events (MACE) were analyzed during(6.3 ± 1.5) months of follow-up in patients with STEMI. Results: Serum levels of hs-CRP in the STEMI group at baseline,24 and 48 hours after operation were significantly higher than those in the SAP group and the healthy group(P < 0.05),while levels of SDF-1α were all significantly lower(P < 0.05). Spearman rank correlation revealed that levels of SDF-1α and hs-CRP at baseline,24 and 48 hours after operation were inversely correlated(r = -0.208,P < 0.05;r = -0.341,P < 0.05;r = -0.384,P < 0.05). MACE took place in 24 patients in STEMI group during follow-up. Compared with those of patients without MACE,fasting glucose(FBG),serum creatintine(Cr) and levels of hs-CRP-SDF-1α at three points in MACE group were statistically significant(P < 0.05). Hs-CRP levels at baseline,24 and 48 hours and SDF-1α levels at baseline were independent predictors of MACE when adjusted by FBG and Cr. Conclusion: SDF-1α is probably a cardioprotective factor with antiinflammatory effect. SDF-1α levels at baseline may have short-term predictive value for MACE after AMI.

Abstract:Objective: To study the immediate protective effect of intensive atorvastatin treatment on coronary artery disease (CAD) patients in the perioperative period of percutaneous coronary intervention (PCI). Methods: Sixty CAD patients were randomly divided into two groups. The levels of creating kinase MB(CK-MB),cTroponin T (cTnT) were measured before and 24 h after PCI. Results: The frequencies of CK-MB > 3 UNL(14.3% vs 9.4%) and CK-MB > 1 UNL(21.4% vs 9.4%) was insignificant in the conventional treatment and intensive atorvastatin treatment groups at 24 h after PCI(CK-MB > 3 UNL,OR:0.62,95%CI:0.13~3.05,P = 0.695;CK-MB > 1 UNL,OR:0.38,95%CI:0.09-1.69,P = 0.281). In the two groups,the occurrences of cTnT > 3 UNL(7.1% vs 6.3%) and cTnT > 1 UNL (14.3% vs 12.5%) were not remarkable at 24 h after PCI(cTnT > 3 UNL,OR:0.62,95%CI:0.13~3.05,P = 0.695;cTnT > 1 UNL,OR:0.86,95%CI:0.19~3.80,P = 1.000). Conclusion: For the CAD patients,the enhanced atorvastatins in the perioperative period of PCI would not further reduce the rates of myocardial necrosis and infarction.

Abstract:Objective: To study the venographic characteristics of posterior ventricular vein(PVV) and explore its value in left ventricular lead implantation in cardiac resynchronization therapy(CRT). Methods: Retrograde cardiac venography with balloon through the communication branch was performed in 78 consecutive patients. Number of branches of cardiac coronary vein,the diameter of PVV,the distance of PVV to the coronary sinus ostium were measured. The pacing threshold of left ventricular lead implanted in PVV was recorded. Results: Retrograde coronary sinus venography was successfully done in all 78 patients. In average,these patients had 1.63 ± 0.49 vessel branches with diameter over 4French. PVV was seen in 40(51.3%) patients. Left ventricular leads were successfully implanted in 22(28.2%) patients in PVV. The average diameter of PVV was (2.03 ± 0.57) mm;the distance of PVV to the coronary sinus ostium was (6.73 ± 6.25) mm,with an angle of (127 ± 27.1)°. Communication branch imaging method was conducive to show PVV,ultra-elective insertion of the sheath into the PVV increased the success rate of left ventricular lead implantation. Pacing threshold of left ventricular lead in the 22 patients was (1.1 ± 0.4)V. Conclusion: The PVV joins the coronary venous near the coronary sinus ostium;communication branch imaging methods is conducive to show PVV. PVV is suitable for left ventricular lead implantation in cardiac resynchronization therapy.

Abstract:Objective:To evaluate the effects of disease duration and body weight on insulin secretion in type 2 diabetes. Methods:Type 2 diabetic patients were divided to groups according to the disease duration,and three insulin secretion indices (HOMA-β,INSR30,INSR120) of all groups were compared with the newly diagnosed group and the parameters in overweight/obese patients were compared with non-obese patients with same disease duration. Results: The insulin secretion ability decreased gradually as the disease duration extended. The statistical differences appeared when the disease duration exceeded 2 years. Insulin secretion was significantly higher in overweight/obese patients than that of non-obese one with the same disease duration. INSR30 and INSR120 increased 9.40% and 5.39% in overweight/obese patients with 2 to 4 years diabetes,respectively,when compared with newly onset group. Conclusion: Insulin secretion ability decreases with type 2 diabetes duration. There are differences of both insulin secretion ability and mode of change between overweigh/obese patients and non-obese one.

Abstract:Objective: To investigate the effect of ankle joint function rehabilitation therapy for cerebral function reconstruction and paralysis limbs function in patients with acute cerebral infarction(ACI) using functional magnetic resonance imaging (fMRI). Methods: Before and after treatment,BOLD-fMRI examinations were performed by 1.5T MRI to 11 ACI patients with rehabilitation therapy,5 ACI patients without rehabilitation therapy respectively. Five healthy volunteers act as controls. The block stimulation function testing was introduced to observe the changes in cerebral activated areas and activated volume and Fugl-Meyer movement function scores. Results: The areas activated by healthy side ankle joint of ACI patients moving were similar to the normal group. When paralyzed side ankle joint moving,in addition to contralateral cerebral hemisphere region(SM1),the ipsilateral SM1 and PMC areas were also activated in varying degrees. The activated volume in the contralateral hemisphere is larger than that in the ipsilateral volume(P < 0.05). After treatment,the activated volume in the contralateral SM1 region was increased obviously(P < 0.05). The activated volume of patients with rehabilitation therapy was more significantly increased(P < 0.05). The Fugl-Meyer movement score was increased after treatment,and the increase of score was more significant in the patients with rehabilitation therapy,compared to the group without treatment(P < 0.01). Conclusion: The fMRI can display objective reconstruction feature of brain after ACI. Early stage moving function rehabilitation therapy of ankle is beneficial to reconstruction of cerebral function and recovery of paralysis limbs function.

Abstract:Objective: To evaluate the roles of TGF-β1 and CD105 in the occurrence and development of cervical condyloma acuminateum (CA) and cervical carcinoma (CC),and determine the potential value of CD105 in the antiangiogenic targeted therapy. Methods: The expressions of TGF-β1 and CD105 were investigated by immunohistochemistry Envision method in samples from 42 cases of CA,50 cases of CC,and 20 cases of normal cervical mucosa as controls. The HPV virus was detected by the polymerase chain reaction (PCR-fluorescence method) analysis. Results: HPV DNA was detected in 20% of controls,100% of CA and 92% of CC,respectively (P < 0.01). TGF-β1 was expressed in 30% of controls,52.4% of CA and 62% of CC,respectively (P < 0.05). TGF-β1 expression only positively correlated with the CC clinical stage (P < 0.01). There was a significantly difference of MVD marked by CD105 antibody among cervical CA,CC and controls(P < 0.01). There was a significantly difference between MVD and size of cervical CA (P < 0.05),and histological grade(P < 0.01),and clinic stage of CC (P < 0.05),respectively. A significantly correlation was confirmed between TGF-β1 and MVD in both CA and CC(P < 0.05). Conclusion: These data provide evidences that TGF-β1/CD105 system plays an important role in abnormal micro-angiogenesis in the occurrence and progress of cervical CA and CC. CD105 could be taken as a potential antiangiogenic target in the treatment of cervical CA and CC.

Abstract:Objective: To investigate the clinical value of active immunotherapy in the treatment of IVF-ET RSA. Methods: We retrospectively analyzed 62 cycles of IVF/ICSI RSA among 42 infertile couples during July 2003 to April 2011.The RSA patients were treated by active immunotherapy combined with tocolysis using HCG or Progesterone after pregnancy. Results: The number of embryo transferred was 116,the average number of embryo transferred was 1.87 ± 0.61,and the endometrial thickness of HCG day was (9.08 ± 2.03) mm. The achievement rate was 64.29%(27/42),the pregnancy achievement rate behind immunotherapy treatment was 96.43%(27/28),the embryo implantation rate was 28.45%(33/116),the biochemical pregnancy rate was 50.00%(31/62),the clinical pregnancy rate was 45.16%(28/62),the miscarriage rate was 3.57%(1/28),the live birth rate was 24.19%(15/62),the cesarean section rate was 93.33%(14/15). Conclusion: In IVF/ICSI RSA,the active immunotherapy can improve the patient immunity reactivity,effectively reduce the miscarriage rate,and improve the successful pregnancy. So it is a safe and simple treatment.

Abstract:Objective: To explore the relationship between the methylenetetrahydrofolate reductase (MTHFR) gene C677T and A1298C polymorphism and unhealthy gravidity history. Methods: Fifty-three women with unhealthy gravidity history and fifty-seven controls were genotyped for MTHFR gene C677T and A1 298C polymorphism using sequencing technique. Results: In the control group,there was 3 of 677T/T genotype and 9 of 677C/T genotype meanwhile 1 298A/C genotype;in the experiment group,there was 9 of 677T/T genotype and 18 of 677C/T genotype meanwhile 1 298A/C genotype. There was an obvious difference between the experiment and the control group in the frequency 677T/T genotype,and 677C/T genotype with 1 298A/C genotype (P < 0.05). Conclusion: MTHFR C677T and A1 298C mutation allele may be related to the increased risk of unhealthy gravidity history.

Abstract:Objective: To compare the pre- and post-neoadjuvant intervention chemotherapeutic expression levels of thymidylate synthase(TS),dihydropymidine dehydrogenase(DPD),thymidine phosphorylase(TP),and methylenetetrahydrofolate reductase (MTHFR),and to explore the relationship between the expression of these four genes and the neoadjuvant intervention chemotherapeutic effects. Methods: Forty-nigh patients with advanced gastric cancer were divided into neoadjuvant chemotherapeutic group (39 patients) and operative group (10 patients). The gene expression levels of TS,TP,DPD,and MTHFR in biopsy specimens from gastroscope and tumor tissue specimens after surgical resection were detected by real-time PCR. Results: According to the results of histopathology,neoadjuvant chemotherapeutic patients were divided into chemotherapeutic effective group (27 cases) and noneffective group (12 cases). The effective rate of neoadjuvant chemotherapy was 69.2%. The expression levels of four detected genes in the tumors of effective patients before neoadjuvant intervention chemotherapy were generally lower than that of the noneffective cases,and the level of DPD decreased 51.2%(P < 0.05). After neoadjuvant intervention chemotherapy,the levels of TS,TP and DPD in the effective group were 50.3%,51.3%,and 31.2% lower than those of noneffective group,respectively (all P < 0.05). The expression levels of TS and TP in effective group decreased by 38.6% and 48.7% compared with that before chemotherapy,respectively (P < 0.05),while the levels of four genes in noneffective group exhibited no significant difference between before and after chemotherapy (P > 0.05). Conclusion: The patients with low level of DPD expression in tumors before neoadjuvant chemotherapy and low levels of TS,TP,DPD after neoadjuvant chemotherapy were sensitive to the neoadjuvant intervention chemotherapy. And the decreased levels of TS and TP after neoadjuvant chemotherapy were important for evaluating the prognosis of neoadjuvant chemotherapy.

Abstract:Objective: To explore the in vitro antitumor effect of cisplatin-loaded micelles on osteosarcoma cell line U-20 s. Methods: Cisplatin-loaded micelles were prepared by a nano-precipitation method with poly (ethylene glycol)-polycaprolactone (mPEG-PCL) as drug carrier. In vitro cytotoxicity of cisplatin-loaded micelles against osteosarcoma cell line U-20s was assessed by MTT assay. Results: The size of cisplatin-loaded micelles is less than 100 nm. The drug loading content is nearly 5% with the encapsulation efficiency more than 18%. In vitro stability test shows cisplatin-londed micelles are stable at routime time. In vitro release study indicates that cisplatin can be released from the core-shell structure of polymeric micelles in a sustained manner. In vitro cytotoxicity assessment shows that free cisplatin and cisplatin-loaded micelles display similar time and dose dependent cytotoxicity against osteosarcoma cell line U-20s. The IC50 value of cisplatin-loaded micelles is bigger than that of free cisplatin at each corresponding time. Conclusion: In vitro evaluation of cisplatin-loaded micelles demonstrates effective antitumor effects compared with free drugs. Therefore,development of nanosized drug delivery systems emerges as a novel field in the research of anticancer drugs,which merits more intensive studies and reveals potential application.

Abstract:Objective: To investigate the effects of cytokine IL-13 on proliferation and invasion of human colon cancer LS174T cells. Methods: LS174T cells were treated with 20 ng/ml recombinant human IL-13 as final concentration. The proliferative and invasive abilities of the cells were determined by MTT assay and Transwell invasion assay,respectively. Results: The MTT result showed that the proliferative capacity of IL-13 treatment group was significantly higher than that of control group(P < 0.05). IL-13 treatment group produced significantly more invasive cells than that of control group. Conclusion: Recombinant human IL-13 at a certain concentration could induce the proliferation and invasion of LS174T cells.

Abstract:Objective: To evaluate the correlation between low field strength MRI and/or color flow doppler imaging(CDFI) and the arthroscopic or intra-operative staging of cartilaginous degeneration of knee joints. Methods: Fifity eight patients for knee joint arthroscopic examination or operation were included. A total of 290 articular surfaces were examined by low filed MRI to evaluate the lateral and medial epicondyle of femur,the intercondylar fassa,and the lateral and medial slope of trochlea. Among them,32 joints were received CDFI examination. Results of MRI and CDFI examination were comparied with that of arthroscopic examination. Results: Taken the arthroscopic examination as gold standard,the correlation index of MRI and CDFI was 0.800(P < O.05) and 0.667(P < O.05),respectively,while combined MRI with CDFI,the correlation index was 0.821(P < 0.05). Conclusion: Both low field MRI and CDFI have good correlation with arthroscope in the diagnose and staging of cartilaginous degeneration of knee joints. The correlation index of combination is higher than single modality. These results implied a role of low field MRI and CDFI in the diagnosis and preoperative assessment of cartilaginous degeneration of knee joints

Abstract:Objective: To explore the application of ARIMA model on prediction of hepatitis E in Jiangsu Province. Methods: Eviews 5.0 software was performed to construct the ARIMA mode based on monthly incidence of hepatitis E from 1997 Jan to 2009 Dec in Jiangsu province,and the constructed model was applied to predict the monthly incidence in 2010. Results: ARIMA(3,1,1)(1,1,0)12 exactly fitted the incidence of the previous time series,and got a good result on the predictive incidence in 2010. Conclusion: The ARIMA model can be used to make a short-term prediction and a dynamic analysis on hepatitis E.

Abstract:Objective: To explore risk factors for anorexia in children. Methods: A community-based 1:4 matched case-control study was conducted using questionnaire survey and vitamin A detection. Conditional logistic regression was used to estimate odds ratio. Results: Multivariate conditional Logistic regression analysis showed that Feeding without ration (OR=1.500,95%CI=1.071~2.101,P = 0.018),Partiality for a particular kind of food(OR=1.780,95%CI=1.267~2.501,P = 0.001),Be forced to feed by paterfamilias(OR=2.133,95%CI= 1.491~3.054,P < 0.001),Be ill with digestive diseases(OR=3.106,95%CI=1.955~4.933,P < 0.001),Be ill with respiratory infection in recent two weeks(OR=1.669,95%CI=1.112~2.513,P = 0.013),Feeding hardly just after birth(OR=1.946,95CI=1.284~2.950,P = 0.002) and Be lack of vitamin A (OR=3.222,95%CI=1.660~6.256,P = 0.001) are associated significantly with anorexia in children. Conclusion: Prevention and control measurements should be focused on the bad habits of children,nutritional knowledge of their parents and timely vitamin A rich foods intake to reduce the risk of anorexia.