Abstract:Objective:To observe the protective effect of anti-LINGO-1 antibody on the motor and sensory axons after mice spinal cord injury (SCI). Methods:Anti-LINGO-1 antibody was administrated by intraperitoneal injection to the mice after a precise 1.1 mm depth dorsal laceration on the 9th thoracic spinal segment was generated by LISA (Louisville injury systems apparatus). Immunohistochemistry or immunofluorescence was used to detect the changes of biotin dextran amine(BDA) labeled descending axons and cholera toxin B subunit(CTB) labeled ascending axons. Results:In anti-LINGO-1 antibody treated mice,a few BDA positive fibers grew into or beyond the lesion gap,and then,extended to the caudal white matter. The longest one reached the site of 1.5 mm caudal to the lesion epicenter. At the same time,more CTB positive sensory fibers were found at the edge of lesion gap than the control group,but no CTB positive fibers extended into or beyond the lesion gap. In IgG treated control mice,neither BDA nor CTB positive fibers extended into or beyond the lesion gap. Conclusion:Systemic injection of the anti-LINGO-1 antibody promotes CST axonal regeneration and limits the dying back of CTB labeled sensory axons. The results indicate that severed CST axons have more robust response to anti-LINGO-1 antibody treatment than CTB labeled sensory axons and suggest that anti-LINGO-1 antibody may serve as a new potential drug for the therapeutic study of SCI.

Abstract:Objective:To construct a recombinant adenovirus vector containing SMOC1 gene and to identify its expression in cultured human aortic valve interstitial cells (hAVICs) and effects on cell proliferation. Methods:The SMOC1 gene was amplified by PCR and inserted into pGEM-T Easy vector and sequenced. Double digested with Not I and Xho I,SMOC1 gene was inserted into pShuttle-CMV vector. This recombinant plasmid was linearized by Pme I and electronically transfected into BJ5183 germs to get the recombinant adenovirus vector (pAdEasy-SMOC1). Then the recombinant adenovirus expressing SMOC1 (Ad-SMOC1) was obtained by packaging in HEK293 A cells. After infecting hAVICs by Ad-SMOC1,the expression of recombinant SMOC1 was detected by Western blot and Immunofluorescence. The proliferation of hAVICs infected with Ad-SMOC1 was measured by MTS assay. Results:The recombinant adenovirus Ad-SMOC1 was successfully generated,which could effectively infect hAVICs and increase SMOC1 protein levels in hAVICs. Besides this,exogenous expressed SMOC1 was mainly located at the cytoplasm and membrane of hAVICs. MTS assay showed that recombinant SMOC1 promoted the proliferation of hAVICs. Conclusion:The bioactive recombinant adenovirus Ad-SMOC1 has been successfully constructed and recombinant SMOC1 promotes cell proliferation. This study laid a foundation for further research of the function of SMOC1 in hAVICs.

Abstract:Objective:Estrogen-mediated neuroprotection in the nigrostriatal dopamine system,depend on the estrogen receptors (ERs),is now widely accepted. In the present study,the role of the two ERs in MPTP toxicity was investigated. Methods:Adult male wild type (WT),ERs knock out (αERKO and βERKO) mice were treated with MPTP. To determine the degree of bradykinesia,pole test was performed. Quantitative real time PCR was used to examine selective genes of the dopaminergic system. Results:Our data demonstrated that administration of 1-methyl-4-phenyl-1,2,3,6-tetrapyridine (MPTP,11 mg/kg) to WT mice led to depletion of DA,DOPAC and HVA. The degree of DA depletion in the αERKO mice was greater than WT mice. Behavioral testing showed that MPTP-injected mice exhibited motor deficits in the pole test. After 2 days of MPTP treatment,a significant prolongation of T-LA was observed in αERKO mice. We also observed that mRNA expression of ERα was significantly increased in the striatum and midbrain in MPTP-treated animals,while the expression of ERβ decreased. The expression of tyrosine hydroxylase(TH),dopamine transporter(DAT) and vesicular monoamine transporter subtype 2(VMAT2) mRNA were decreased in αERKO and βERKO mice. The down-regulation of TH and DAT mRNAs by MPTP in the midbrain acted on both ERα and ERβ,whereas MPTP treatment lead to decreased mRNA expression of VMAT2 via ERα. Conclusion:Our results suggested that estrogen acting via ERs is an important regulative factor for the nigrostriatal dopamine system,ERα may play a more significant role.

Abstract:Objective:To screen anti-EBV-LMP1 Fab of high specificity and affinity from a phage antibody library and identify its binding activity. Methods:Subtractive screening method by alternating SUNE-1/LMP1 cells and solid-phase from a large capacity Fab phagelibrary. After eight rounds screening,30 positive clones were identified by ELISA test. The positive clones were selected by Western blot for Fab soluble expression in TOP 10F’ and the binding activities of Fab with LMP were analyzed in HNE2/LMP1 cell lines. Results:A Fab fragment with fine activity to LMP1 was selected,purified and expressed. The anti-LMP1 Fab binding specificity was confirmed by cell ELISA,FACS,immunofluorescence and immunohistochemistry. Conclusion:The anti-LMP1 Fab binding to nasopharyngeal carcinoma (NPC) cells provides a promising candidate for the biotherapy of EBV-related carcinoma.

Abstract:Objective:To establish a GST pulldown assay that can detect the activated Rab35 in eukaryotic cells. Methods:We constructed prokaryotic expression vector containing fusion protein GST-RUN. The correct plasmid was transfected into Ecoli. BL21 stain and the expression of the fusion protein was inducted by IPTG. SDS-PAGE and Western blotting were utilized for determination of the corresponding recombinant proteins. Then we pulled down the fusion protein with glutathione beads to detect the activated Rab35 in HEK293T cells. Results:The prokaryotic expression vector GST-RUN was successfully constructed and expressed fusion protein GST-RUN stably. GST pulldown assay showed high activated Rab35 in HEK293T cells. Conclusion:The activated Rab35 was successfully detected by GST pulldown assay. This experimental scheme can be used for further study of active Rab35 targeting in eukaryotic cells.

Abstract:Objective:To construct PLJM1-p53-GFP plasmid of mouse p53 gene and study the role of p53 in the prostate cancer cells PC3. Methods:The open reading frame(ORF) of mouse p53 gene was amplified from mouse 3T3-L1 cells by RT-PCR,and inserted into the PLJM1-NRG1-GFP vector. The recombinant plasmid was transformed into DH5α competent E. coli. The positive clones were screened by PCR and the inserts were confirmed by DNA sequencing. The PLJM1-p53-GFP plasmid was transfected into the prostate cancer cells PC3. QPCR was used to detect the efficiency of the transfection. The invasive activity and proliferation of PC3 cells were measured after transfecting the PLJM1-p53-GFP plasmid. Results:DNA sequencing demonstrated that the recombinant plasmid PLJM1-p53-GFP was constructed successfully. The invasive activity and proliferation of PC3 cells were obviously decreased after transfection of the PLJM1-p53-GFP plasmid. Conclusion:The plasmid of PLJM1-p53-GFP of mouse p53 gene was successfully constructed. It can be used in the functional research for cancer.

Abstract:Objective:To construct the RNAi vectors targeting mouse NOMO1 gene and compare the silence effects of these small interference RNAs,and to study the effects of NOMO1 gene on the differentiation of P19 cells to cardiac myocytes. Methods:Short hairpin RNAs of mouse NOMO1 gene were designed by Designer3.0 software,synthesized and cloned into the pGPU6/Hygro plasmid. Connection products were transfected into competent cells and identified by PstⅠ+BamHⅠ double digestion and DNA sequencing. Then the vectors were transfected into P19 cell. The level of NOMO1 mRNA was evaluated by RT-PCR. The expression of α-MHC mRNA in P19 cell,which was induced by DMSO,was evaluated by RT-PCR. Results:shRNA was inserted into the pGPU6/Hygro plasmid correctly,confirmed by double digestion and DNA sequencing. The strains of P19 cell stably expressing shRNA were selected. RT-PCR and Western blot showed that 4 shRNAs silenced the expression of NOMO1 gene markedly. α-MHC was down-regulated in transfected P19 cell. Conclusion:pGPU6/Hygro vectors carried mouse NOMO1 shRNA are constructed successfully. It provides us a useful tool for the study of NOMO1 gene function in the differentiation of P19 cell into cardiac myocyte. NOMO1 may be involved in α-MHC gene expression,and the differentiation of P19 stem cell into mesodermal cell.

Abstract:Objective:To investigate the effect of human parathyroid hormone(PTH)on alveolar bone of ovariectomized(OVX) rats. Methods:Sixty female 5-month-old rats were divided into three groups randomly,two groups,which were ovariectomized and developed into osteoporotic models,were named as PTH treated group(PTH group) and the control group(OVX group). Another group was sham ovariectomized(sham-OVX) as the blank control group(sham-OVX group). Six weeks later,the PTH group was injected with recombinant human parathyroid hormone(1-34)[rhPTH(1-34)]once daily for forty days,while the other two groups were injected with saline. Then they were sacrificed and their mandibles were separated respectively for radiological imaging,histomorphological observation and osteoclastic cell couting. Results:The radiological imaging analysis showed that the loss of the distal alveolar bone of the first molar in OVX group was more than that of either PTH group or sham-OVX group[(1.34 ± 0.89)mm vs. (0.78 ± 0.62)mm,(0.67 ± 0.54)mm],both P < 0.05. Meanwhile,the histomorphological observation suggested that the bone of OVX group was absorbed more than the other two groups. Besides,the cell counting results indicated that OVX group had more osteoclast than both PTH group and sham-OVX group(38.6 ± 20.2 vs. 23.3 ± 13.0 and 25.0 ± 11.4 respectively,both P < 0.01),which showed significant statistical differences. Conclusion:The absorption of alveolar bone of the osteoporotic rats can be inhibited by PTH.

Abstract:Objective:To observe the effect of spironolactone on oxidative stress of glomerular mesangial cells induced by high glucose,and explore whether the MAPKs family signaling pathway was involved in this effect. Methods:DHE fluorescence staining was used to evaluate the intracellular production of reactive oxygen species(ROS),NBT kit was used to detect the intracellular SOD activity,and Western blot was applied to identify the phosphorylated and total protein expression of MAPKs family genes,such as ERK,JNK and p38MAPK in cultured human mesangial cells (HMC) treated with high glucose or spironolactone. Results:DHE fluorescence staining showed that the production of ROS in HMC was markedly increased with the treatment of high glucose,while which was significantly decreased in the stimulation of spironolactone. Compared to the treatment of high glucose,intracellular SOD activity was significantly increased(P < 0.05) with the treatment of spironolactone. Western blot analysis suggested that the high sugar significantly increased the expression of phosphorylation of ERK and p38MAPK in HMC cells,while given the treatment of spironolactone,these effects were reversed(P < 0.05). Furthermore,there were no remarkable differences among the groups in phosphorylated JNK,total protein level of ERK,p38MAPK,and JNK. Conclusion:Spironolactone may play a role in reducing the production of intracellular ROS and increasing the activity of SOD and antagonism of oxidative stress induced by high glucose via down-regulation of the phosphorylation level of MAPK family signaling. All these actions may contribute to the protection of the glomerular mesangial cells and postponement of kidney glomerulus sclerosis.

Abstract:Objective:To investigate the potential involvement of CEACAM6 in the invasion and chemo-resistance of pancreatic cancer cell SW1990 by inducing epithelial-mesenchymal transition(EMT). Methods:After establishing and screening,CEACAM6-overexpressing cell line SW1990-CEACAM6 was observed by inverted microscope,and the changes of EMT markers were determined by Q-PCR and western blot,the invasion ability was detected by transwell,the chemo-sensitivity to gemcitabine was determined by MTT assay. Results:Q-PCR and Western blot confirmed that SW1990-CEACAM6 cell line was established successfully. Overexpression of CEACAM6 made the pancreatic cell line SW1990 prone to mesenchymal phenotype,such as up-regulation of vimentin and down-regulation of E-cadherin. SW1990-CEACAM6 cell has greater activity of invasion and chemo-resistance to gemcitabine (P < 0.05) than SW1990-EGFP cell. Conclusion:CEACAM6 could promote EMT and enhance malignant potential in terms of invasion and chemo-resistance in pancreatic cancer cell.

Abstract:Objective:To evaluate the correlation of doxorubicin and cisplatin intervention with the expression levels of MDR1 and MRP1 in anaplastic thyroid carcinoma cell line HTh74. Methods:Doxorubicin resistant cells (HTh74/DOX) and cisplatin resistant cells(HTh74/DDP) were obtained by treating the HTh74 cell with doxorubicin or cisplatin for 6 months respectively. Fluorescent quantitative RT-PCR was adopted to detect the transcription of MDR1 and MRP1 in HTh74/DOX and HTh74/DDP. Results:Compared to their parental HTh74 cell,HTh74/DOX cell expressed high level of MDR1 and low level of MRP1,HTh74/DDP expressed high level of MRP1 and low level of MDR1. HTh74/DOX cells lacked cross resistance to cisplatin,and HTh74/DDP cells lacked cross resistance to doxorubicin. Inhibitors of MDR1 or MRP1 partly restored the toxicity of doxorubicin or cisplantin on the resistant cells respectively. Conclusion:Different chemotherapeutics increase the expression of different ABC transporters to enhance the drug resistance. Detecting the expression of ABC transporters can be used to predict the effect of chemotherapeutic drugs in clinical. The proportionate inhibitor of ABC transporter combined with chemotherapeutic drugs may be effective in individual treatment.

Abstract:Objective:To investigate the feasibility of gene transfection of Hela and A549 cells mediated by cationic polymer-polyethylenimine(PEI) and evaluate the effect of cell cycle status on efficiency of PEI-mediated gene delivery. Methods: Hela and A549 cells were synchronized into different phases of the cell cycle by 6 MV X ray radiation or drugs or serum deprivation. The synchronized cells were transfected using PEI carrier at various PEI/DNA ratio(P/D=2/2,4/2,6/2,8/2,10/2-滋g/-滋g),and the transient transfection efficiency of enhanced green fluorescence protein (EGFP) gene in these cells was determined by flow cytometry and fluorescent microscopy. The relationship between cell cycle status and the efficiency of transgene expression was analyzed. Results:Hela cell could be synchronized in S phase by X ray radiation or incubation with Cisplatin(PDD),and A549 cell could be synchronized in G2/M phase by incubation with Docetaxel(TXT) at certain dose-time condition. Both Hela and A549 cells were synchronized in G0/G1 phase serum deprivation. Transfection efficiency in synchronized cells was different at different phases of the cell cycle. PEI showed the minimal transfection efficiency at G0/G1 phase and the medium at G2/M phase and the maximum at S phase(P < 0.05,S,G2/M phase group vs control; P < 0.0001,S,G2/M phase group vs G0/G1 phase). Conclusion:PEI is an effective and safe carrier for gene delivery. Cell cycle status restricted the expression efficiency of transfection mediated by PEI. Transfection using PEI is influenced by cellular uptake,transportation into nucleus and transcription,and those processes are cell-cycle-dependent.

Abstract:Objective:To investigate the possible role of miR-181a/b in the development of cisplatin resistance in human lung cancer cell line A549/CDDP. Methods:Using quantitative real-time PCR analysis for miRNA and Western blot to detect the expression of miR-181a/b and anti-apoptotic protein BCL2,MCL1 and XIAP in cisplatin resistant human lung cancer cell line A549/cisplatin (CDDP) and its parental A549 cell,respectively. The luciferase reporter plasmids carried 3＇-untranslated region of BCL2,MCL1 and XIAP were constructed to testify the target genes of miR-181a/b,respectively. Using transient transfection of miR-181a/b to mimic the up-regulation of miR-181a/b in A549/CDDP cells and observe the effect of miR-181a/b on the expression level of BCL2,MCL1,XIAP and cisplatin resistance phenotype. Using flow cytometry to detect CDDP-induced apoptosis of the A549/CDDP cells after the transfection. Results:miR-181a/b was down-regulated in cisplatin-resistant human lung cancer cell line A549/CDDP,the down-regulation of miR-181a/b was concurrent with the over-expression of its targeted anti-apoptotic genes BCL2,MCL1 and XIAP in A549/CDDP cells,compared to the parental A549 cell line. The luciferase activity of BCL2,MCL1 and XIAP 3＇-untranslated region-based reporters construct in A549/CDDP cells suggested that BCL2,MCL1 and XIAP were the common target genes of the miR-181a/b. Over-expression of miR-181a/b sensitized A549/CDDP cells to CDDP. Overexpression of miR-181a/b also inhibited the expression of BCL2,MCL1 and XIAP and sensitized A549/CDDP cells to CDDP-induced apoptosis. Conclusion:miR-181a/b could enhance the sensitivity of cisplatin in human lung cancer cell line A549/CDDP,at least in part,by modulation of apoptosis via targeting multiple anti-apoptosis genes.

Abstract:Objective:To investigate the expression and clinical significance of ADP-ribosylation factor 6(ARF6) in hepatocellular carcinoma (HCC). Methods:The expression of ARF6 was determined by tissue microarray and immunohistochemistry in 150 cases of HCC and 48 cases of adjacent cirrhosis tissue. Results:The positive expression of ARF6 in HCC and adjacent cirrhosis tissue were 48.7% (73/150) and 8.3% (4/48) respectively(P < 0.05). The positive expression of ARF6 in HCC was closely related to the portal vein invasion,clinical stage,tumor diameter and AFP level. The results also indicated that a high expression level of ARF6 was associated with worse recurrence-free survival. The median recurrence-free survival was substantially reduced among patients with positive ARF6 protein(36 months vs. 45 months,P = 0.019 1). Conclusion:The expression of ARF6 is closely correlated with the clinical prognosis of HCC. It suggests ARF6 may be a potential prognostic biomarker and therapeutic target for HCC.

Abstract:Objective:To investigate the correlation between the expression of MRP1/CD9 and the biological behaviors of gastric cancer. Methods:Tissue microarray and immunohistochemistry technique were used to detect the expression of MRP1/CD9 in 223 cases with gastric cancer. Results:The positive rate of MRP1/CD9 in gastric cancer patients was 58.3%,and the expression was not associated with gender,age,infiltration depth and stages,but had close relationship with the differentiated degree and lymphnode metastasis. The expression of MRP1/CD9 in moderate or high differentiated group was higher than that in the low differentiated group significantly (P < 0.05);which was the same when compared between non-lymphnode metastasis group and lymphnode metastasis group(P < 0.01). Forward stepwise binary logistic regression showed:lymphnode metastasis,which was first into the equation,is the most significant factor of MRP1/CD9 expression and followed by differentiation. Conclusion:The change of MRP1/CD9 expression in gastric cancer was closely correlated with the differentiated degree and lymphnode metastasis.

Abstract:Objective:To discuss the pregnancy and obstetric outcome of the patients after transcervical resection of septum(TCRS). Methods:We collected 166 patients with uterus septa from May 2006 to May 2011 from Nanjing Maternity and Child Health Hospital of Nanjing Medical University,and compared the pregnancy and obstetric outcome between pre-operation and post-operation with medical records,surgical records and telephone follow-up. Results:No serious complications occurred during operation,and the shortest duration from the remove of IUD to pregnancy was one month. After TCRS,the rate of spontaneous abortion decreased from 64.53% to 15.25%(P < 0.01),while the live birth rate increased from 1.89% to 72.03%(P < 0.01). The rate of cesarean section was 80%,in which the cesarean section without indications was 41.12%. Besides,the incidence of postpartum hemorrhage was 23.53%,in which the rate of blood transfusion patients was 45% including one threatened uterine rupture case in our study. Conclusion:TCRS could significantly promote the pregnancy and improve outcomes after surgery. Intensive pregnancy monitoring and sufficiently guidance were essential to improve the obstetric outcome and prevent postpartum hemorrhage.

Abstract:Objective:To explore the effect on the urination and bowel function of nerve-sparing modified hysterectomy and traditional hysterectomy in pre-menopausal or postmenopausal women. Methods:In total,150 cases were treated by the nerve-sparing modified hysterectomy (study group),and 150 cases were treated by traditional abdominal hysterectomy (control group). All uterine samples with cardinal ligament,sacral ligament,vesicocervical ligament were obtained. Expressions of S-100 in those attached ligaments were evaluated by immunohistochemistry technique in order to investigate the contents of nerves. According to whether the patient in premenopausal and menopausal period,the two groups were divided into three parts:premenopausal patients,early menopausal patients(menopausal period was no more than 5 years),late menopausal patients (menopausal period was more than 5 years). Their urination and defecation in one month and one year after operation were compared. Results:Contents of nerves in ligament stump of the study group were significantly lower than those of controls. There was no significant difference in operative time,intra-operative blood loss and length of postoperative hospital stay between the two groups. The incidence rates of frequent micturition,delay of urination,urinary incontinence and constipation in the study group were lower than those in the control group in one month and one year. Compared between the two groups,there was no statistically significance in pre-menopausal,early menopausal patients(P > 0.05),except the incidence rate of frequent urination,and delay of urination in one month in early menopausal patients. But in the late menopausal patients,the incidence rates of frequent urination,delay of urination and constipation in the study group were significantly different from those in the control group in one month and one year. Conclusion:Compared with the traditional hysterectomy,reformed operation can reduce the damage of disposable bladder and bowel nerve and depress the incidence of frequent urination,delay of urination,urinary incontinence and constipation. It was particularly significant in elderly patients.

Abstract:Objective:To analyze the efficacy of comprehensive therapy mainly using hysteroscopic adhesiolysis by mini-scissors. Methods:In a retrospective study,122 patients were received hysteroscopic adhesiolysis,which was carried out by mini-scissors,and monitored by ultrasonography. After operation,they received intrauterine devices(IUD) placement,with a following cycling hormonal therapy. After 3 months therapy uterine cavity and menstrual flow were evaluated. Results:Menstrual flow was normal in 68.0% of patients and uterine cavity returned to be normal in 109 patients,accounting for 89.3%. Re-operation was needed in 13 patients because of mild or moderate adhesion. Pregnant rate was 52.8% during the first year. Conclusion:The comprehensive therapy for intrauterine adhesion has good effect and deserves to be recommended in clinic.

Abstract:Objective:To explore the relationship between long term hemodialysis patients’ nutritional status and interdialytic weight gain(IDWG),in order to provide the guidance for preventing of the patients’ malnutrition. Methods:From 2008 August to 2007 July,123 maintenance hemodialysis patients in the end stage of chronic kidney disease of our hospital were divided into 3 groups according to IDWG%(IDWG/dry weight). Group A:IDWG%<3%;Group B:3%5%. Six serologic markers and six human body parameters were selected as nutrition indexes to evaluate the patients nutrition status. A retrospective study was adopted to investigate nutritional indexes at the three time points:before study (in the beginning),amid study (at the end of the sixth month),after study(at the end of twelfth month),and single factor analysis of variance was used to evaluate statistical significance difference among the three groups. At the same time,rank correlation analysis was also used to study the relationship between different IDWG% and all nutritional indexes. Results:Nutritional indexes were compared to each other among the three groups respectively in the beginning,in the middle and at the end of study;Single factor analysis of variance revealed no statistically significant differences(P > 0.05). Correlation analysis revealed no statistically significant correlation between different IDWG% and each nutritional index(P > 0.05). Conclusion:There was no relationship between IDWG% and the patients’ nutritional status,and no definite statistically significant correlation between IDWG and each nutritional index. The results suggest that relative low level IDWG% do not affect the patients’ long term nutritional status.

Abstract:Objective:To explore the clinical application of Conners’ kiddie continuous performance test (K-CPT) on diagnosis in preschool children with attention deficit hyperactivity(ADHD). Methods:Using the diagnostic and statistical manual of Mental disorders (DSM-IV) as a gold standard,K-CPT and parent symptom questionnaire(PSQ) were tested in 41 cases of preschool children with ADHD and evaluated. Results:Compared with DSM-IV,the sensitivity for K-CPT in diagnosing ADHD reached 73.3%,specificity for K-CPT was 81.8%,the false negative rate was 26.7%,and the false positive rate was 18.2%. The coincidence rate amounted 75.7%. The value of kappa was 0.470 (P < 0.001). There is no significant difference(P > 0.05) in the detection rate of K-CPT and PSQ. Discussion:K-CPT could be used as an objective diagnostic tool and applied in diagnosis of preschool ADHD.

Abstract:Objective:To determine the frequency of chronic kidney disease(CKD) and it’s associated cardiovascular risk factors in type 2 diabetic patients. Methods:A cross-sectional study was conducted in Jiangsu Province Official Hospital from January 2008 to December 2009. eGFR was calculated using the Modification of Diet in Renal Disease(MDRD) study equation. CKD was defined as the presence of albuminuria or eGFR < 60 ml/(min·1.73 m2). Albuminuria was defined as urinary albumin-to-creatinine ratio(ACR)≥30 mg/g. Risk factors associated with CKD were evaluated using logistic regression. Results:In total,1 521 outpatients with type 2 diabetes mellitus[(mean age (63.9 ± 12.0)years] were recruited. The frequency of CKD and albuminuria was 31.0% and 28.9% in the study cohort,respectively. After adjusted by sex and age,hypertension,duration of diabetes and anemia were significantly associated with CKD with odds ratio of 1.932(95% CI:1.275~2.929,P = 0.002),1.029(95% CI:1.001~1.058,P = 0.040) and 1.696 (95% CI:1.089~2.639,P = 0.019),respectively. Conclusion:CKD and albuminuria were common in type 2 diabetes. Strategies to prevent or delay progression of kidney disease in type 2 diabetes,including antidiabetic,antihypertensive and improving anemia,should be carried out early.

Abstract:Objective:To improve the latent syphilis diagnostic criterion through investigating 836 cases of latent syphilis. Methods:Collecting the data of 836 cases of latent syphilis in 6 national sexually transmitted diseases (STD) sentinel surveillance in Jiangsu province from 2008 to 2010,moreover,the serums of the cases were collected to check the diagnostic veracity with non treponemal antigen serological test (TRUST) and treponemal antigen serological test (ELISA). Results:The average age of 836 cases latent syphilis was(42.65 ± 13.26) years old;87.92% cases of the latent syphilis were came from comprehensive medical institutions;82.66% cases were checked out with to be hospitalized;55.86% cases were firstly treated with penicillin;92.35% cases were ELISA and TRUST positives simultaneously and 27.28% cases of TRUST titer were more than 1∶8. Conclusion:In order to attain the aims of prevention and control of syphilis in planning of China(2010-2020),the diagnosis and therapy abilities of latent syphilis must be improved.