• Volume 0,Issue 1,2013 Table of Contents
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    • >南京医科大学学报(自然科学版)
    • Hyperm ethylation of miR-200c promoter correlated with EGFR-TKI resistance in lung cancer cells

      2013(1):1-5. DOI: 10.7655/NYDXBNS20130101 CSTR:

      Abstract (3117) HTML (48) PDF 470.95 K (2201) Comment (0) Favorites

      Abstract:Objective:To examine the relationship between miR-200c promoter methylation and the response of non small cell lung cancer(NSCLC)cells to(TKI). Methods:NSCLC cell lines H1650,HCC827 with EGFR 19 deletion and cell lines H358 and H1299 with wild type EGFR were used in this study. The methylation of miR-200c promoter region was examined by methylation-specific PCR (MSP). The response of different cell lines to gefitinib was examined by cell counting kit-8 (CCK-8)assay. The expression of miR-200c was examined by RT-PCR. When treated with 5-aza-CdR,the response to gefitinib and miR-200c expression of four cell lines H1650,HCC827,H358 and H1299 were observed. Results:HCC827 and H358,which were sensitive to gefitinib,highly expressed miR-200c with promoter unmethylated. H1650 and H1299,which were insensitive to gefitinib,expressed low levels of miR-200c with promoter methylated. When treated with 5-aza-CdR,H1650 and H1299 expressed higher miR-200c and were more sensitive to gefitinib than before (P < 0.05),while HCC827 and H358 had no alteration in miR-200c expression and the sensibility to gefitinib(P > 0.05). Conclusion: As methylation of gene promoter suppressed the expression of miR-200c, the lung cancer cell lines H1650 and H1299 can be insensitive to gefitinib.

    • Effects of POLⅠ on radiosensitivity of human esophageal cancer cell line and its sensitivity to cisplatin

      2013(1):6-10. DOI: 10.7655/NYDXBNS20130102 CSTR:

      Abstract (2356) HTML (81) PDF 637.85 K (2112) Comment (0) Favorites

      Abstract:Objective:To investigate the effect of POLⅠ (DNA polymeraseⅠ)on the radiosensitivity of human esophageal cancer cells (ECA-109)and their sensitivity to cisplatin. Methods:The plasmid expressing shRNA against POLⅠ was constructed and transfected into ECA-109 cells. The transfection efficiency was evaluated by fluorescence microscopy. POLⅠ expression was detected by Real-time Fluorogenic Quantitative PCR. The cell sensitivity to X-ray was detected by colony formation assay. MTT was used to determine the sensitivity of ECA-109 cells to cisplatin. Results:After successful transfection of constructed shRNA-POLⅠ gene into ECA-109 cells,a large number of fluorescent particles were observed in fluorescence inverted microscope. The expression of POLⅠ in mRNA was significantly reduced (P < 0.05). The ability of radiosensitivity was enhanced. On the other hand,sensitivity to cisplatin was enhanced (P < 0.05). Conclusion:ShRNA-POL I in human esophageal cancer cells could enhance their radiosensitivity and sensitivity to cisplatin.

    • >南京医科大学学报(社会科学版)
    • How to make our own life happier?

      2013(1):7-9. DOI: 10.7655/NYDXBSS20130103 CSTR:

      Abstract (2653) HTML (65) PDF 259.22 K (2393) Comment (0) Favorites

      Abstract:n real life,moral individual may not have a happy life. Therefore,the unity of individual moral and happiness needs the guarantee of the happiness new deal. More importantly,individuals must play their subjectivity role to enhance happiness. On the basis of demonstration of happiness could be learned,the article considered that “positive knowledge,achieving happiness”,“above reality,lifting of boredom” and “enlarge the happiness,reduce the unfortunate” were the three main ways of attaining happiness.

    • Research of home pension security system from the perspective of happiness

      2013(1):10-12. DOI: 10.7655/NYDXBSS20130104 CSTR:

      Abstract (2019) HTML (51) PDF 217.35 K (2017) Comment (0) Favorites

      Abstract:主观幸福观和心理幸福观是两个重要概念,分别为“生活善”与“人生善”两个方面-文章首先探析了老年人“生活善”在达到一定程度后与幸福感弱相关,其次分析了“人生善”是影响老年人幸福的重要因素,最后从幸福视角探讨“三位一体”居家养老保障体系的构建及意义-

    • >南京医科大学学报(自然科学版)
    • Construction of recombinant lentiviral vector of PI3KCG,identification of its expression in neonatal rat cardiomyocytes and prelimary function test

      2013(1):11-15. DOI: 10.7655/NYDXBNS20130103 CSTR:

      Abstract (2532) HTML (48) PDF 420.78 K (2296) Comment (0) Favorites

      Abstract:Objective:To construct lentiviral expression vector contained phosphatidylinositol 3-kinase p110 gamma(PI3KCG), and identify its expression in neonatal rat cardiomyocytes and detect its prelimary function. Methods:The PI3KCG lentiviral vector plasmid (PLV-PI3KCG)was constructed by homologous recombination method. The three plasmids of PLV-PI3KC and of lentivirus system were co-transfected into human embryonic kidney 293T cells by using lipofectamine. The expression of green fluorescent protein (GFP)in the control group was examined by using fluorescent microscope at 24 h and 48 h after transfection. Recombinant PLV-PI3KCG plasmid was successfully identified in 293T cells detected by polymerase chain reaction (PCR). The viral supernatant was collected with 72 h after transfection. The ischemia/reoxygenation (I/R)injury model of neonatal rat myocardial cells was established. Myocardial cells isolated from SD neonatal rats were random division into five groups after being cultured for 3d: the normal control group, the I/R group, the null vector positive control group, the PI3KCG transfection preconditioning group and the PI3KCG transfection+ Ly294002 group. Various techniques were adopted to detect the products of cells and cellular:the cardiomycytes beat frequency, the levels of myocardial cells viability rate and the levels of lactate dehydrogenase (LDH). Results:The PLV-PI3KCG plasmid was constructed and expressed in the 293T cells successfully. Compared with the I/R group, the myocardial cells viability and cardiomycytes beat frequency of the PI3KCG transfection preconditioning group were significantly increased (P < 0.01,respectively), and released LDH were significantly decreased (P < 0.01,respectively). Conclusion:PLV-PI3KCG plasmid was expected to become an available vector to investigate PI3K/Akt pathway in the cardiocytes ischemia reperfusion injury process.

    • >南京医科大学学报(社会科学版)
    • A study on John B. Grant’s beneficiation to public health of China

      2013(1):13-17. DOI: 10.7655/NYDXBSS20130105 CSTR:

      Abstract (2175) HTML (51) PDF 297.89 K (2766) Comment (0) Favorites

      Abstract:John B. Grant was a world-known public health scientist. He was born in China,and created his public health projects in China. In Peking Union Medical College,he created the first department of public health. He established the first health demonstration to build urban community health service system in Beijing. In Ding County,Hebei province,he guided Chen Zhiqian to build China’s first rural primary care health system. He played an important role in the development of public health undertakings in China,and his experiences in China also gained the respect of the world’s public health scholars.

    • >南京医科大学学报(自然科学版)
    • The EP3 receptor splice variants expression patterns in liver cancer cell and the relationship to the growth of liver cancer cell

      2013(1):16-21. DOI: 10.7655/NYDXBNS20130104 CSTR:

      Abstract (2403) HTML (56) PDF 412.99 K (2296) Comment (0) Favorites

      Abstract:Objective:This experiment sought to investigate the EP3 receptor splice variants expression patterns in liver cancer cell lines and their functions in controlling hepatoma cell growth . Methods:WST assay was used to detect the growth of liver cancer cell induced by EP3 receptor agonist Sulprostone;Two-dimensional electrophoresis and mass spectrometry were used to detect the protein level in CCLP1 cells by EP3 receptor agonist sulprostone;Real-Time PCR was used to detect the expression of EP3 receptor splicing variants in liver cancer cell. Results:CCLP1 cells were treated with 10 μmol/L sulprostone for 24 h,the results of cell growth rate increased to 131.46%(P < 0.01)detected by WST assay;CCLP1 cells were treated with 10 μmol/L Sulprostone for 24h,two-dimensional electrophoresis and mass spectrometry were used to detect the protein level. The results showed that promoting cell proliferation,invasion and cell metabolism related proteins were increased,on the contrary that reducing the cell metabolism rate,slowing down the cell growth,invasion and inhibiting cell metabolism related proteins were reduced;EP3-4,EP3-5,EP3-6 and EP3-7 splice variants were observed in HUH-7,Hep3B,HepG2,HuCCT1 and CCLP1 cells. Conclusion:EP3 receptor promoted the proliferation of liver cancer cell by up-regulating proteins that promoting cell growth and metabolism at same time down-regulating proteins that inhibiting cell growth and metabolism. EP3-4,EP3-5,EP3-6 and EP3-7 splice variants were observed in HUH-7,Hep3B,HepG2,HuCCT1 and CCLP1 cells.

    • >南京医科大学学报(社会科学版)
    • Formation of the public medical system and influence on the medical education in the Republic of China

      2013(1):18-21. DOI: 10.7655/NYDXBSS20130106 CSTR:

      Abstract (2142) HTML (45) PDF 271.62 K (2041) Comment (0) Favorites

      Abstract:Because of lack of the medical resources,some insight put forward the public medical system in the Republic of China. The scholars proposed a detailed guidance on the meaning and the specific methods,a big part of them matched the medical education. After the successful experiment in some individual medical school,the public medical system was taken shape initially that effect the medical education at that time.

    • On the support of New Fourth Army for medical and health services in Anti-Japanese Based Area in Central China

      2013(1):22-26. DOI: 10.7655/NYDXBSS20130107 CSTR:

      Abstract (2016) HTML (50) PDF 320.23 K (2235) Comment (0) Favorites

      Abstract:During the war of resistance against Japan,the Anti-Japanese Based Area was damaged by the prevalence of different kinds of diseases. New Fourth Army actively supported the work of local medical care,such as treatment for masses,establishment of local medical institutions,implementation of epidemic prevention campaign,mobilization of civil traditional medicine,development of health habits of the masses,training of local medical staff. All these measures reduced the disease pain of masses in the base area,covered the sick and wounded soldiers,solved the medical problem of local workers,closed relations between the army and people,and expended the influence of New Fourth Army.

    • >南京医科大学学报(自然科学版)
    • Both transient and sustained gene expression profile alterations of bone marrow mesenchymal stem cells induced upon co-cultured with myeloma cells

      2013(1):25-31. DOI: 10.7655/NYDXBNS20130106 CSTR:

      Abstract (2068) HTML (51) PDF 606.13 K (2337) Comment (0) Favorites

      Abstract:Objective:Recent researches suggested that the abnormalities of bone marrow microenvironment have important implications for the genesis and evolution of multiple myeloma. However,the gene expression profile (GEP)changes in the interaction between bone marrow mesenchymal stem cell(MSC) and myeloma cells are not reported. Methods:To investigate the pathogenesis of multiple myeloma,cDNA microarray was employed to investigate the GEP of MSC before and after interacting with myeloma cells. And the genes with differential expression MSC co-cultured with myeloma cells (the MC groups)or cultured alone to continue after the removal of MM cells (MA groups)compared with the control groups (the MK groups)were screened by scanning and analyzed by computer software. Results:After co-cultured of MSC with MM cells (the MC groups),among total 10 000 genes,we filtered a total of 837 differential representing genes (837/10 000,8.37%),of which there were 472 up-regulated genes (472/837,56.39%)and 365 genes were down-regulated(365/837,43.61%). We found 367 differentially expressed genes in the MA groups compared to the control ones,including 218 up-regulated genes (218/367,59.40%)and 149 down-regulated genes(149/367,40.60%). Then we selected eight genes(MMP-1,FGFR2,ANGPTL4,MFAP5,TGM2,STC1,CCL7 and IL-32)from differential genes to verify by real-time quantitative PCR and their functions were explored. The Real-time quantitative PCR results were consistent with cDNA microarray results. Conclusion: It is indicated that myeloma cells induce a variety of gene expression changes of normal bone marrow MSC,and some changes still exist even after the removal of MM cells. In addition,among the eight selected genes in this study,seven of them have not been reported to be involved in the pathogenesis of multiple myeloma.

    • >南京医科大学学报(社会科学版)
    • Research commentary on our country’s medical insurance payment reform

      2013(1):27-30. DOI: 10.7655/NYDXBSS20130108 CSTR:

      Abstract (2469) HTML (83) PDF 250.77 K (2463) Comment (0) Favorites

      Abstract:In recent years,academia,media and government paid huge attention to the problem of medical insurance payment system reform from different angles. The academia studied the payment methods continuously,and obtained many achievements. This paper summarized the viewpoint of scholars,analyzed the necessity of the reform,classification of the payment methods and the development trend,and then evaluated the scholars’ research progress in the final.

    • Enlightenment and meaning of safe supervision mode of food supplied for Hongkong

      2013(1):31-35. DOI: 10.7655/NYDXBSS20130109 CSTR:

      Abstract (2086) HTML (50) PDF 299.68 K (2091) Comment (0) Favorites

      Abstract:National food safety events have sprung out in recent years,which reveal defects in domestic “farm to table” food industrial lines as well as its supervision. Beginning with the study of safe supervision mode of food supplied for Hongkong,the paper analyzed condition and issues of national food supervision system. In view of valuable experience about Hongkong food and domestic current political and economic environment,the paper also showed relatively appropriate way of reformation in food safety supervision regime and pointed out reasonable,scientific,systematic optimization and suggestions.

    • >南京医科大学学报(自然科学版)
    • Characterization and potential application of monoclonal antibody specific to monomeric C-reactive protein

      2013(1):32-36. DOI: 10.7655/NYDXBNS20130107 CSTR:

      Abstract (2138) HTML (66) PDF 471.53 K (2184) Comment (0) Favorites

      Abstract:Objective:To prepare monoclonal antibody (MAb)against monomeric C-reactive protein. Methods:BALB/c mice were immunized with mCRP-BSA and the spleen cells of the mice were fused with myeloma cells SP2/0 at the ratio of 5∶1. Monoclonal hybridomas were screened by competitive indirect enzyme-linked-immunoadsorbent assay (ELISA)and limited dilution. Mouse ascites were prepared by intraperitoneal injection of hybridoma cells. MAbs to mCRP was purified by Protein G Affinity chromatography methods. The specificity and activity of ascites antibody were determined by Western Blot and indirect ELISA. The protective effects of this antibody influenced on cardiomyocytes were further studied. Results:Strain of hybridoma cell line could secrete MAb stably. After purification,the obtained monoclonal antibody can decrease mCRP induced TGF--茁 expression on cardiomyocytes. Conclusion:The obtained strain of hybridoma cell line could secrete MAb stably,and purified MAb had protective effect on cardiomyocytes. [Key words] monomeric C-reactive protein;monoclonal antibody

    • >南京医科大学学报(社会科学版)
    • On the quality of life and economic burden of psoriasis and eczema patients

      2013(1):36-42. DOI: 10.7655/NYDXBSS20130110 CSTR:

      Abstract (2572) HTML (45) PDF 312.09 K (2206) Comment (0) Favorites

      Abstract:Objective:To compare disease characteristics,quality of life and economic burden between patients with psoriasis and eczema. Methods:Total 198 patients with psoriasis and 117 patients of eczema were selected for questionnaire survey using SF-36 scale and Skindex-29 scale in Dermatology Hospital of the Chinese Academy of Medical Sciences from June 2011 to March 2012. Results:①A total of 313 questionnaires were valid,of which 196 cases were psoriasis with the male to female ratio is 1.88∶1,and 117 cases in the control group with male to female ratio is 1.25∶1. Psoriasis was compared with eczema in terms of the social behavior,such as smoking,drinking,sleeping,diet limitation,suicide tendency,et al. And we found that psoriasis had a higher percentage than eczema in smoking,sleeping,and suicidal tendency (P < 0.01).②Comparing the quality of life between patients with psoriasis and eczema by SF-36 and Skindex-29 scales,we found that patients with psoriasis had a lower quality of life than patients with eczema (P < 0.01).③The economic burden of psoriasis is more serious than that of the control group. Conclusion:The results of psoriasis and eczema were generally similar to the previous studies. However,psoriasis had a significantly higher proportion of smoking,drinking,suicidal tendencies. Although psoriasis and eczema were both skin diseases,the psoriasis had a much lower quality of life than the eczema,health workers should pay much attention to the quality of life of patients with psoriasis when in diagnosis and treatment. The economic burden of psoriasis was more severe than that of eczema. Therefore,health policy-making department should pay much more attention to psoriasis with severe economic burden.

    • >南京医科大学学报(自然科学版)
    • Preparation and characterization of enterovirus 71 VP1 monoclonal antibody

      2013(1):37-41. DOI: 10.7655/NYDXBNS20130108 CSTR:

      Abstract (2949) HTML (52) PDF 539.35 K (2198) Comment (0) Favorites

      Abstract:Objective:To obtain an anti-enterovirus 71 (EV71)-VP1 monoclonal antibody, and preliminarily analyze its biological characteristics. Methods:Immunized the BALB/c mice with purified recombinant EV71-VP1 protein. The spleen cells were fused with mouse myeloma cells(Sp2/0). Subsequently, limited dilution method was used to screen positive hybridoma cell lines. After several times of subclone, one hybridoma cell line (6F2B9)was obtained. The supematant of 6F2B9 was collected and purified by affinity chromatography with Protein G sepharose. The concentrations of antibodies were tested by BCA method and its subtype was identified by Ig subtype qualitation kit. The titer and specificity of the McAb were detected by indirect ELISA. We used indirect immunefluorescence assay (IFA) to observe specifical combination with EV71 virus. Results:We succeeded in selecting a hybridoma cell line secreting anti-EV71-VP1 monoclonal antibody. The McAb obtained was named DSE-136 and belonged to IgG2b, κ light chain, and its titer was 1∶3.2×105 and could specifically bind to the surface of EV71 which was detected by IFA. Conclusion:We successfully obtained a mouse anti-EV71-VP1 McAb with high dilution and specificity, which has laid foundation on antigen diagnosis and vaccine research and development for EV71.

    • Forecast of SSRs in hemagglutinin sequences of influenza viruses A/H1N1

      2013(1):42-47. DOI: 10.7655/NYDXBNS20130109 CSTR:

      Abstract (2761) HTML (61) PDF 503.29 K (2147) Comment (0) Favorites

      Abstract:Objective:To explore the feasibility of using Autoregressive Integrated Moving Average (ARIMA) model to predict the relative abundance and relative density of simple sequence repeats(SSRs)in Hemagglutinin Sequences of influenza viruses A/H1N1,and to provide scientific basis for measures of preventing and controlling influenza pandemic. Methods:Eviews 6.0 software was utilized to construct the ARIMA model based on the relative abundance and relative density of SSRs in hemagglutinin (HA) sequences of influenza A with high homology from 1970 to 2007,and the constructed model was applied to predict the relative abundance and relative density from 2008 to 2010.The model was evaluated by actual data and then used to forecast the data of 2011. Results:The ARIMA model exactly fitted the relative abundance and relative density of the previous time series,and got a good predicting result on the data of 2008 to 2010. Conclusion:The ARIMA model can be used to make a short-term prediction and a dynamic analysis on the relative abundance and relative density of SSRs.

    • >南京医科大学学报(社会科学版)
    • Investigation of influence of human environment of rehabilitation department on patients’ psychological factors

      2013(1):43-45. DOI: 10.7655/NYDXBSS20130111 CSTR:

      Abstract (1896) HTML (79) PDF 221.52 K (2329) Comment (0) Favorites

      Abstract:Objective:To discuss the influence of construction of the internal environment of rehabilitation department on the psychological status of patients,and propose some suggest to promote the recovery of patients and management of the hospital. Methods:Questionnaires and interviews were used to 256 patients and 96 staff of rehabilitation department of 4 hospitals in Nanjing. The data were analyzed by statistical description and analysis. Results:Compared with patients,the evaluation of doctor-patient relationship in staff was significantly high. The communication factors between doctor and patient and among the patients had greater influence on patients. Conclusion:It is good for the recovery of patients to strengthen the communications between doctor and patient and change the traditional concept of medical service.

    • On human resource management of primary public hospital and the influence factors under the new medical reform

      2013(1):46-49. DOI: 10.7655/NYDXBSS20130112 CSTR:

      Abstract (2075) HTML (70) PDF 272.19 K (2221) Comment (0) Favorites

      Abstract:Objective:To analyze the effect of human resource management and influence factors of primary hospital,and put forward policy suggestions according to the problems. Methods:Questionnaire survey was used to the hospital ward staff. Results:The degree of satisfaction of hospital medical staff was above the average,the degree of hospital culture construction was medium level,and the effect of staff training was poor. The main influence factors were hospital culture,departmental cohesion,assessment system and the attention of the human resource management. Conclusion:The conductor should formulate reasonable salary distribution and evaluation system,strengthen the construction of hospital culture,design of practical training content,consolidate clinical skills of doctors,improve qualified recruitment,and establish the internal cultivation mechanism.

    • >南京医科大学学报(自然科学版)
    • Effects of serotonin on cortical and trabecular bone parameters of female mice detected by Micro-CT

      2013(1):48-52. DOI: 10.7655/NYDXBNS20130110 CSTR:

      Abstract (2517) HTML (61) PDF 529.74 K (2278) Comment (0) Favorites

      Abstract:Objective:To investigate the effects of serotonin of different doses on cortical and trabecular bone parameters of female mice. Methods:Wild-type (wt)C57BL/6 female mice with 2-month-old were injected with serotonin of different doses (0,5,10,20,50 and 100 mg/kg)subcutaneously (s.c.)daily for 3 months. Micro-CT (μCT)was used to quantitatively analyze bone parameters of the fifth lumbar bodies,distal femurs and midshafts of the femurs of each group of mice. Results:Compared to the control groups mice,the 10-100 mg/kg groups had significantly reduced trabecular numbers,trabecular bone volume and surface density,and increased trabecular separation,leading to a decrease of trabecular bone mineral density and bone mineral capacity. Mice in the 50-100 mg/kg groups caused significantly decreased cortical bone mineral capacity secondary to reduced cortical thickness and cortical bone volume density. Conclusion:Serotonin has a significantly negative regulation on bone metabolism in mice.

    • >南京医科大学学报(社会科学版)
    • Investigation and analysis of outpatient service influence factors of specialist hospital by RSR method

      2013(1):50-52. DOI: 10.7655/NYDXBSS20130113 CSTR:

      Abstract (1966) HTML (43) PDF 217.32 K (2058) Comment (0) Favorites

      Abstract:Objective:To investigate and analyze outpatient service influence factors of specialist hospital. Methods:Outpatient service influence factors questionnaire was used to investigate 200 outpatients at random. The data was analyzed by RSR method. Results:Two evaluations were “poor” in terms of traffic conditions of coming to our hospital and waiting for the result of examination. Six evaluations were “medium” in terms of waiting for examination,service of registration and charge,service of pharmacy staff,telling of the important items,waiting for paying and appointment of registration. Seven evaluations were “good” in terms of one-stop service of service station,arrangement of outpatient doctor,layout of outpatient,the direction marks, health promotion,waiting for registration and service of outpatient doctor. Waiting for taking medicine,service of outpatient nurse and environment of outpatient are “excellent”. Conclusion:According to cancer patients’ characteristic,these influence factors should be explored in depth to carry better measures to improve service and meet the demand of outpatients’ diagnosis and treatment.

    • On the problems and countermeasures in emergency medical quality control

      2013(1):53-55. DOI: 10.7655/NYDXBSS20130114 CSTR:

      Abstract (2074) HTML (44) PDF 214.78 K (2428) Comment (0) Favorites

      Abstract:Emergency medical management is faced with lack of unified emergency construction standards,lack of stable and high-quality emergency professionals,lack of sufficient investment and the emergency process is not convenient for critical ill patients. It’s difficult to establish uniform quality standards of construction and management of emergency medical quality control,difficult to unify emergency physician professional level quality control. It’s also difficult in the single disease quality control and in correcting bias from emergency medical quality control. Only by formulating a series of unified emergency department construction standards and emergency professional category,training a professional emergency medical team,establishing rules and regulations,the emergency medical quality control could be successfully promoted after supervised by the department of health administration.

    • >南京医科大学学报(自然科学版)
    • An association study of genetic polymorphisms in complement component C3 and ischemic stroke susceptibility in the ethnic Han of Eastern china

      2013(1):53-58. DOI: 10.7655/NYDXBNS20130111 CSTR:

      Abstract (2745) HTML (75) PDF 452.64 K (2253) Comment (0) Favorites

      Abstract:Objective:To explore the association of genetic polymorphisms in complement component C3 with ischemic stroke risk in the ethnic Han of Eastern China. Methods:A total of 700 cases with ischemic stroke and the control group of 700 cases were recruited. Polymorphisms were rs2277984 G/A,rs37455652 G/C genotyped by TaqMan fluorescence probe. The multivariate Logistic regression model was used to exclude the effects of conventional risk factors on ischemic stroke. Results:The rs2277984 variant was not associated with ischemic stroke risk;the rs3745565 GG/CC genotype,only if under the dominant genetic model and uncorrected,had an increased risk of ischemic stroke (OR = 1.37,95%CI 1.04-1.80,P = 0.026). The rs3745565 variant was not associated with ischemic stroke risk after adjusting confounding factor (P > 0.05). Conclusion:The present study suggested that genetic polymorphisms in complement component C3 (rs2277984 and rs3745565) may not exert influences on ischemic stroke susceptibility among the ethnic Han of Eastern China.

    • >南京医科大学学报(社会科学版)
    • Integrating quality curriculum resources to improve the effectiveness of ideological and political theory courses in medical colleges

      2013(1):61-65. DOI: 10.7655/NYDXBSS20130117 CSTR:

      Abstract (1990) HTML (49) PDF 303.71 K (2234) Comment (0) Favorites

      Abstract:Through the investigation of major influence factors of ideological and political theory courses teaching in medical colleges, the article analyzed the explicit and implicit factors of regulating the effectiveness of ideological and political theory courses, and tried to find the method to integrating quality curriculum resources to improve the effectiveness of ideological and political theory courses in medical colleges.

    • >南京医科大学学报(自然科学版)
    • Association between serum uric acid concentration and the metabolic factors of diabetes

      2013(1):62-67. DOI: 10.7655/NYDXBNS20130113 CSTR:

      Abstract (2246) HTML (69) PDF 425.95 K (2199) Comment (0) Favorites

      Abstract:Objective:To examine the association of serum uric acid level and the incidence of type 2 diabetes. Methods:From 2008 to 2011,1 416 eligibles were collected from the inpatients of Department of Endocrinology and Gerontology in the First Affiliated Hospital of NJMU. The disease history was surveyed,the biochemical indexes were detected and the general physical data were measured. All of the patients were divided into four groups(Ⅰ,Ⅱ,Ⅲand Ⅳ group)according to the level of serum uric acid,and association between uric acid level and metabolic indexes in each group was evaluated. Results:① With the rise of uric acid concentration,the incidences of diabetes in women were gradually decreased. In the first three groups,BMI increased with the rise of uric acid concentration. ② The levels of FPG and PPG were reduced with the increasing of uric acid concentration. PINS,FC-P,PC-P,TG,BUN,and Cr in the higher uric acid group were significantly higher than the lower group. The level of HDL-C was inversely varied with uric acid concentration in the first three groups of quartiles. ③The average uric acid level of participants with normal glucose tolerance was higher than that in the patients with abnormal glucose tolerance. ④ The levels of uric acid were negatively related to HbA1c,FPG and PPG(P < 0.001,= 0.001,< 0.001)and positively related to FINS,PINS,FC-P and PC-P(P < 0.001). ⑤The risk of abnormal fasting blood glucose significantly raised with the elevation of uric acid level,especially in the group Ⅳ,which predicted the increased risk of diabetes (P < 0.001). Conclusion:The serum uric acid level is closely related to gender,age,duration of diabetes,incidence of hypertension and coronary heart disease,BMI,dyslipidemia and glucose. It can be used as a predictive index for diabetes. Elevated level of uric acid is one of the risk factors of diabetes. Within normal range,the increased uric acid level is also a protective factor to glucose metabolism of patients with diabetes. [Key words] serum uric acid;diabetes;relative factors

    • >南京医科大学学报(社会科学版)
    • Application and development of two kinds of education test theory on examination paper quality control and evaluation

      2013(1):66-71. DOI: 10.7655/NYDXBSS20130118 CSTR:

      Abstract (1890) HTML (49) PDF 293.16 K (2335) Comment (0) Favorites

      Abstract:This study summarized the similarities and differences between the classic test theory and the item response theory,illustrated that the illustrate item response theory in foreign education institutions had been widely applied while the examination paper quality control and test construction in domestic medical colleges and universities still relied on classical test theory through the method of literature retrieval,and then advanced the necessity of the innovation of theory and methods.

    • >南京医科大学学报(自然科学版)
    • Analysis on risk factors related to hypertension with diabetes mellitus

      2013(1):68-72. DOI: 10.7655/NYDXBNS20130114 CSTR:

      Abstract (2299) HTML (68) PDF 436.05 K (1776) Comment (0) Favorites

      Abstract:Objective:This study was aimed to explore the risk factors related to hypertension with diabetes mellitus. Methods:Based on logistic regression through a survey with questionnaires and physical measurement,10431 cases with hypertension were conducted. All these cases were screened from 174 villages and cluster sampled in Jianye District in Nanjing,Peixian County in Xuzhou,Haian Country in Nantong and Lianshui County in Huaian. Results:In hypertension cases,the proportion of diabetes is about 9.25%. According to logistic regression analysis,high level of high-density lipoprotein -cholesterol (HDL-C)protected the hypertension patients against diabetes,with odds ratio(OR)=0.711(95%CI:0.570~0.888);as well as triglycerides(TG),total cholesterol(TC),low level of HDL-C,age,education,high BMI were the risk factors inducing hypertension with diabetes,with OR of 1.917 (95%CI:1.663~2.209),2.549(95%CI:2.147~3.206),1.370(95%CI:1.059~1.772),1.211(95%CI:1.108~1.324),1.162(95%CI:1.054~1.281),1.264 (95%CI:1.153~1.386),respectively. Conclusion:It is concluded that overweight and obesity,high level of TG and TC,low level of HDL-C and education level are the risk factors for the hypertension cases with diabetes. Therefore,these factors must be paid more attention in hypertension control and prevention in order to decrease the risk of complicated diabetes and to elevate the quality of life for hypertension cases.

    • >南京医科大学学报(社会科学版)
    • Teaching quality assurance of clinical practice for stomatology majors

      2013(1):72-74. DOI: 10.7655/NYDXBSS20130119 CSTR:

      Abstract (2004) HTML (43) PDF 217.63 K (2600) Comment (0) Favorites

      Abstract:Practice teaching is a very important way for the students of stomatology to train their practical ability,innovative ability and professional quality. At the same time,it is the only way for them to be a real dentist. During the training course,we ensure our clinical practice teaching quality through establishing access system of teaching hospitals,organizing systematic training of teachers,integrating the practice program,and improving supervision system of teaching quality.

    • >南京医科大学学报(自然科学版)
    • Association of I198T,R92H polymorphisms in lipoprotein-associated phospholipase A2 gene with coronary heart disease in the Chinese Han population

      2013(1):73-77. DOI: 10.7655/NYDXBNS20130115 CSTR:

      Abstract (2581) HTML (62) PDF 401.88 K (2139) Comment (0) Favorites

      Abstract:Objective:To investigate the association of I198T and R92H point mutations in lipoprotein-associated phospholipaseA2 (Lp-PLA2)with coronary heart disease(CHD) in the Chinese Han population. Methods:Totally 173 CHD patients and 101 normal controls were genotyped by DNA sequencing instrument. Results:There was no significant difference between the CHD group and the control group in the frequencies of I198T genotypes and allele,but the frequencies of the R92H genotypes and allele were significantly higher in CHD patients than those in normal controls (P < 0.05). The levels of total cholesterol (TC)and low density lipoprotein cholesterol (LDL-C)were significantly higher in the RH+HH genotype group than in the RR genotype group (P < 0.01). The distributions of haplotype IH and IR had a significant difference (P < 0.01)between CHD patients and normal controls. Binary logistic regression analysis demonstrated that polymorphism of R92H was an independent risk factor for CHD. Conclusion:The I198T polymorphism of Lp-PLA2 gene has no significant correlation with coronary heart disease,while the polymorphism of R92H could be associated with risk of CHD in Chinese Han population.

    • >南京医科大学学报(社会科学版)
    • Discussion on problem-based learning combining with scenario-based medical cases in cardiology learning

      2013(1):75-78. DOI: 10.7655/NYDXBSS20130120 CSTR:

      Abstract (2040) HTML (44) PDF 242.66 K (2162) Comment (0) Favorites

      Abstract:Objective:To study the mode of problem-based learning combining with scenario-based medical cases in cardiology learning. Methods:160 interns were randomly divided into experimental and control groups. Experimental group were given by problem-based learning combining with scenario-based medical cases mode,and control group were given question-case discussion mode. An examination was then carried out to evaluate their achievement. Results:Students of experimental group achieved higher scores at choice questions,subjective topic,case analysis and even their total scores (P < 0.05). They believed that the new teaching mode could do better work at focus interest,raising enthusiasm and promoting their skills of communication and collaboration. More participants preferred problem-based learning combining with scenario-based medical cases mode (P < 0.05). Conclusion:Problem-based learning combining with scenario-based medical cases mode played better effect on rising interns not only clinical thinking,but also analysis and solving skills of cardiology.

    • >南京医科大学学报(自然科学版)
    • Results of 2∶1 matched case-control study on congenital heart disease in children and its risk factors in Yili state of Xinjiang province

      2013(1):78-82. DOI: 10.7655/NYDXBNS20130116 CSTR:

      Abstract (2267) HTML (59) PDF 405.83 K (2287) Comment (0) Favorites

      Abstract:Objective:To investigate the distribution of congenital heart disease (CHD)in children aged 0~14 years in Yili state. With the exploration possible environmental and genetic risk factors relevant to the occurrence of CHD. This study provided scientific guidelines for the prevention of CHD. Methods:In this population-based 2∶1 matched case-control study,442 children from eight counties and one city in Yili state were diagnosed as CHD,and 884 children from same districts were sampled randomly as the control group. Observation,heart auscultation and echocardiography examination were performed in the children. Single-factor analysis and multi-factors Logistic regression analysis were applied to analyze the risk factors of CHD. Results:Based on the single-factor analysis and multi-conditional Logistic regression model,eight risk factors related with the incidence of CHD were figured out. Conclusion:The early stage of pregnancy is the critical period for fetal cardiac development. Environmental and genetic factors played important roles in the occurrence of CHD. The etiologic research had great significance for diminishing the prevalence of CHD. Avoiding the exposure of environmental risk factors and strengthening pregnant care were beneficial to reduce the development of CHD.

    • >南京医科大学学报(社会科学版)
    • Reformation and exploration of promoting the ability of scientific research innovation in urological graduate students

      2013(1):79-82. DOI: 10.7655/NYDXBSS20130121 CSTR:

      Abstract (1759) HTML (46) PDF 237.03 K (2299) Comment (0) Favorites

      Abstract:The medical graduate students are high-level medical talents in China. Through retrospective analysis of the education work of graduate students’ innovation ability in Department of Urology,the First Affiliated Hospital of Nanjing Medical University in recent years,we realized that high quality medical research talents could be cultured by full use of the advantages of the clinical departments and better management of training in research innovation ability,which could adapt to the need of current society to medical talents and play an important role in promoting the development of department.

    • Investigation of published medical thesis from laboratory medicine students of adult higher medical education

      2013(1):83-85. DOI: 10.7655/NYDXBSS20130122 CSTR:

      Abstract (1936) HTML (71) PDF 201.39 K (2098) Comment (0) Favorites

      Abstract:Article written and published is an important indicator to measure the research and innovation capacity of students of adult medical education. In the form of an anonymous questionnaire, published medical thesis from 78 laboratory medicine students of adult higher medical education was investigated. The percentage of students who published articles was 28.2%(22/78). The students published articles had work experiment of 5 to 20 years, average 15.4 years. Additionally, 74.4%(58/78) students wanted to publish paper in the next five years because of promotion of professional titles. Those students not published did not know how to write. The rate of publishing article of adult laboratory medicine education student was lower, so we should offer some course of article writing skills.

    • >南京医科大学学报(自然科学版)
    • Changes of connexin 43 distribution in myocardial cell of the left ventricle in the cases of sudden-death due to hyperthyroid heart disease

      2013(1):86-89. DOI: 10.7655/NYDXBNS20130118 CSTR:

      Abstract (2280) HTML (64) PDF 478.09 K (2333) Comment (0) Favorites

      Abstract:Objective:To investigate the relationship between the expression of myocardial connexin 43 (Cx43)in myocardial cell of the left ventricle in the cases of sudden-death due to hyperthyroid heart disease. Methods:Selected 26 autopsy cases from the Department of Forensic Medicine School of medicine,Yangtze University during 2010 to 2012. The cases were divided into two groups,13 cases in each group. ①Experimental group:9 cases male,4 female;age range is 20 to 52 with average age is 34.7 years old,Suddenly died of hyperthyroid heart disease and sudden death. ②Control group:7 males,6 females,age range is 22-60 with average age is 38.8 years old. After conventional pathological examination,Cx43 in the left ventricular muscle of each case was detected with immunohistochemic staining and image analysis. Results:In control group,the Cx43 positive staining were found in the intercalated disc. In experimental group,the Cx43 positive staining were found in the cytoplasm or side-side connection of ventricular myocyte,and the positive area of Cx43 positive particles were significantly decreased than control group (P < 0.01). Conclusion:The expression of myocardial connexin (Cx43)was significantly decreased in sudden-death patients with hyperthyroid heart disease,which suggests the decreased expression of Cx43 may have relation with sudden death of hyperthyroid heart disease.

    • >南京医科大学学报(社会科学版)
    • Correlation analysis of test score of physiology & biochemistry course and level of MHK of Xinjiang Uighur students

      2013(1):86-88. DOI: 10.7655/NYDXBSS20130123 CSTR:

      Abstract (2700) HTML (55) PDF 210.62 K (2352) Comment (0) Favorites

      Abstract:Objective: To evaluate the relationship between the test score of medical physiology & biochemistry course and the level of Chinese minority Chinese level testing (MHK) of Xinjiang Uighur students,analyze the causes and improve the quality of teaching. Methods:602 Uighur students enrolled in the Xinjiang Medical University in 2011 were examined,and their average scores were compared. Results:There were no significant difference between students with MHK level three (31.6,n=10) and MHK level four (39.03,n=22),and there were significant difference among students with MHK level five (47.24,n=70),six(49.44,n=140),seven (52.88,n=246) and eight (52.77,n=114). Conclusion:It could be seen that the better level of MHK of Uighur students have the higher passing rate in the course. The level of Chinese language proficiency could predict the performance of ethnic minority students on learning basic medical science courses.

    • Application of Sandwich teaching method based on cases to the experimental teaching practice of forensic clinical medicine

      2013(1):89-92. DOI: 10.7655/NYDXBSS20130124 CSTR:

      Abstract (2132) HTML (76) PDF 272.29 K (2284) Comment (0) Favorites

      Abstract:The traditional teaching method was not so effective in forensic clinical medicine due to the complexity of experimental teaching content,which lead to our attempt of applying Sandwich teaching method based on cases to the experimental teaching practice of forensic clinical medicine,with the aim to motivate students and improve their abilities of expression and communication as well as abilities to analyze and solve problems. As a result,the experimental teaching quality of forensic clinical medicine was enhanced,and students’ comprehensive capabilities were cultivated.

    • >南京医科大学学报(自然科学版)
    • Clinical manifestation,endoscopic and histological features of abdominal type Henoch-Schonlein purpura

      2013(1):90-93. DOI: 10.7655/NYDXBNS20130119 CSTR:

      Abstract (2492) HTML (45) PDF 757.62 K (2354) Comment (0) Favorites

      Abstract:Objective:To analyze the gastrointestinal manifestation of abdominal type Henoch-Schonlein purpura (HSP),including clinical,endoscopic and histological features. Methods:There were 24 patients with a final diagnosis of abdominal type HSP admitted to our hospital from June 2005 to June 2012. Their medical records,including clinical presentation,laboratory data,endoscopy and pathology reports,were reviewed retrospectively. Results:The common gastrointestinal symptoms of HSP were abdominal pain (95.8%),gastrointestinal bleeding (87.5%),nausea and vomiting (37.5%)and diarrhea (8.3%). In the cases of 14 patients the skin eruptions occurred after the onset of abdominal symptom from 1 to 30 (mean 13.7 ± 9.8) days. There were 19 of 24 patients underwent endoscopy. Duodenal lesions were found in 14(73.7%) patients,followed by stomach lesions in 6(31.6%),ileum lesions in 6(31.6%),jejunum lesions in 5(26.3%),esophagus lesions in 2(10.5%) and colorectal lesions in 2(10.5%). The endoscopic lesions included mucosal diffuse hyperemia edema,bleeding spots,submucosal hemorrhage and hematoma,erosion,multiple irregular ulcers and nodular changes. Histological manifestations showed swollen vascular endothelial cells of capillary vessels,nonspecific inflammation with infiltration of neutrophil and lymphocytes. Typical vasculitis was found in one case. Conclusion:The skin eruptions may occur after the onset of abdominal symptom,which results from the difficulty of early diagnosis of abdominal type HSP. Typical clinical manifestations and endoscopic features can be helpful in the early diagnosis of abdominal type HSP.

    • Changes and implications of inflammatory mediators in serum and exhaled breath condensate in patients with pulmonary hypertension associated with COPD

      2013(1):97-101. DOI: 10.7655/NYDXBNS20130121 CSTR:

      Abstract (2524) HTML (63) PDF 369.69 K (2288) Comment (0) Favorites

      Abstract:Objective:To estimate the relationship between inflammation and pulmonary hypertension in patients with chronic obstructive pulmonary disease,we investigate the levels of C-reactive protein (CRP)and interleukin-6 (IL-6)in serum and exhaled breath condensate (EBC)in chronic obstructive pulmonary disease (COPD)with or without pulmonary hypertension. Methods:EBC and serum of 37 cases in PH associated with COPD and 48 cases in single COPD were collected. Lung function,arterial blood gases and echocardiography were assayed in all cases. ELISA was used for detecting the levels of IL-6 in EBC and serum. Particle-enhanced turbidimetric immunoassay was used for analyses the levels of hs-CRP in EBC and serum. Results: ①The levels of CRP[(17.16 ± 6.25)mg/L]and IL-6[(79.67 ± 18.72)ng/L]in serum in patients with pulmonary hypertension were significantly higher than those in patients without pulmonary hypertension (P < 0.001). ②The levels of CRP[(2.03 ± 0.46)mg/L mg/L]and IL-6[(8.75 ± 2.03)ng/L]in EBC in patients with pulmonary hypertension were significantly higher than those in patients without pulmonary hypertension (P < 0.05). ③PASP in the PH group was positively correlated with the levels of CRP and IL-6 both in serum and EBC(r = 0.415~0.875,P < 0.001). Conclusion: The inflammation reaction of system and local respirary tracts plays an important role in the pathogenisis of pulmonary hypertension in COPD.

    • Comparison study with ERCC1 and RRM1 to predict clinical efficacy of chemotherapy in NSCLC patients

      2013(1):105-109. DOI: 10.7655/NYDXBNS20130123 CSTR:

      Abstract (2416) HTML (65) PDF 395.22 K (2118) Comment (0) Favorites

      Abstract:Objective:To compare the clinical efficacy of chemotherapy in late stage NSCLC by two types of methodologies:selective gemcitabine and cisplatin (GP)treatment based on ERCC1 and RRM1 low expression vs. non-selective standard treatment. Methods:This prospective,open study was based on whether target tissue is appropriate for immunohistochemical detection of molecular markers. The enrolled late stage NSCLC patients were divided into two groups,including the group A:low expression in ERCC1 and RRM1 markers,and the group B:no molecular marker pre-selection. After 4-6 weeks treatment with GP chemotherapy,the clinical outcome,progressive-free survival(PFS),overall survival(OS)and toxic/adverse reactions from chemotherapy were compared between the two groups. Results:The Group A (GP method based on ERCC1 and RRM1)has shown significant improvement in efficacy (χ2 = 5.377,P = 0.02) as well as statistically significant extension in PFS (P = 0.03)comparing to the Group B. There was no significant difference in OS and toxic/adverse reactions between the two groups. Conclusion:GP chemotherapy method which selected based on ERCC1 and RRM1 low expression could significantly improve the efficacy and PFS in patients of NSCLC late stage.

    • Evaluation of diet quality of residents in Jiangsu province with china diet balance index

      2013(1):116-120. DOI: 10.7655/NYDXBNS20130126 CSTR:

      Abstract (2508) HTML (61) PDF 347.10 K (2243) Comment (0) Favorites

      Abstract:Objective:By using diet balance index (DBI-07),the diet structure and quality of resident in Jiangsu province have been evaluated. The findings can be used for nutritional intervention. Methods:The basic information of 954 residents aged 18-65 years who had completed 3 days dietary information were selected from 2009 China Health and Nutrition Survey. The quality of dietary was evaluated by using DBI-07 method. Results:The overall dietary quality of Jiangsu residents was somewhat imbalance. Insufficient intake and excessive intake were both coexisted in the residents of Jiangsu. Male had a higher level of excessive intake and imbalance than female. The situation of dietary deficiencies and imbalance of rural areas were more severe than those of the city. Group age from 40 to 50 has the highest level of dietary imbalances. Conclusion:For Jiangsu residents,intakes of fruits,milk and beans were insufficient,whereas red meat,edible oil and salt intake were excessive. Major dietary pattern of residents is the Pattern B. Three main target populations for intervention were male,rural residents and residents aged 40-50 years. Educations on dietary guidelines and food guide pagoda are needed. Meanwhile the implementations of dietary intervention regarding the populations excessive intake with salt and oil are recommended.

    • Combination of radionuclide myocardial imaging and coronary angiography in diagnosis and management of coronary artery disease

      2013(1):121-125. DOI: 10.7655/NYDXBNS20130127 CSTR:

      Abstract (2773) HTML (61) PDF 368.26 K (2077) Comment (0) Favorites

      Abstract:Objective:To explore the usefulness of 99mTc-MIBI single photon emission computed tomography(SPECT)combined with coronary angiography (CAG) in diagnosis and management of coronary artery disease (CAD). Methods:One hundred and seventy-six suspected patients with coronary artery disease underwent CAG or CAG plus SPECT. The management strategy and revascularization procedure(Percutaneous transluminal coronary angioplasty[PTCA]vs. coronary artery bypass graft surgery[CABG]) were prospectively selected on the basis of a combination of CAG,SPECT and the approval of diagnostic criteria of CAD of the Ministry of Health. Patients received PTCA or CABG were retested with SPECT in 1~3 months after treatment. The results were compared for exploring the role and significance of them in preoperative diagnosis and postoperative follow-up for CAD. Results:A total of 176 patients underwent CAG,of which 113 patients were performed SPECT for one week before CAG. The results showed that 138 patients of CAD were found from them. The results of patients with CAD were analyzed,the diagnostic sensitivity,specificity and accuracy of CAG were 93.4%(129/138),97.4%(37/38)and 94.3%(166/176),respectively. The ratios of SPECT were 85.2%(69/81),90.6%(29/32)and 86.7%(98/113)and of CAG + SPECT(as long as one is abnormal,it should be deemed positive) were 100%(81/81),87.5%(28/32)and 96.5%(109/113),respectively. Sixty-five patients received SPECT within 3 months after treatment(PTCA 63,CABG 2). It was showed that △STR and △LVEF(Percentual Systolic Wall Thickening and left ventricular ejection fraction change between pre- and post- operation)were related to chest pain relief of CAD patients. Conclusion: Combination of SPECT and CAG can improve the diagnostic sensitivity and accuracy of CAD;SPECT can be intuitive,quantifiable,repeatable and convenient to reflect the improvement of clinical symptoms of CAD patients after treatment.

    • Diagnosis and evaluation of bone metastasis with PET/CT and bone scintigraphy in patients with malignancies

      2013(1):126-130. DOI: 10.7655/NYDXBNS20130128 CSTR:

      Abstract (4286) HTML (86) PDF 628.46 K (2173) Comment (0) Favorites

      Abstract:Objective:To evaluate diagnostic value of PET/CT with 18F-FDG and whole-body bone scintigraphy (BS)with 99mTc-MDP for metastatic bone tumor in patients with malignancies. Methods:A total of 75 patients with malignances,average of 59.85 ± 13.59 years old,were included in this study. All patients underwent PET/CT imaging with 18F-FDG and BS with 99mTc-MDP for two weeks. Based on the diagnostic results of patients,we calculated the sensitivity,specificity,positive predictive value (PPV),negative predictive value (NPV),and accuracy of PET/CT imaging and BS method to detect bone metastasis. Moreover,image features on different body regions were assessed and compared between two imaging methods. Results:There were 34 patients diagnosed with skeletal metastasis among all 75 patients. The sensitivity of PET/CT was 94.1%(32/34) in detecting bone metastasis. The specificity of PET/CT was 95.1%(39/41)The PPV,NPV and accuracy of PET/CT were 94.1%,95.1% and 94.7%,respectively. For BS with 99mTc-MDP,the sensitivity of detecting skeletal metastasis was 91.2%(31/34)and the specificity was 65.9%(27/41). The PPV,NPV and accuracy of BS were 68.9 %,90 % and 77.3 %,respectively. On basis of region analysis,the main abnormal uptakes of 18F-FDG were located in spine,rib and pelvis by PET/CT detection. For BS with 99mTc-MDP,the main lesions were located in rib,spine and bones of limbs. Conclusion:The specificity,accuracy and PPV of PET/CT imaging were higher than those of BS. However,there was no significant difference in sensitivity and NPV between PET/CT imaging and BS.

    • Diagnostic efficiency of real-time tissue elastography in evaluating liver fibrosis

      2013(1):131-134. DOI: 10.7655/NYDXBNS20130129 CSTR:

      Abstract (2548) HTML (82) PDF 466.06 K (2195) Comment (0) Favorites

      Abstract:Objective:The purpose of this study is to analyze and discuss the efficiency of real-time tissue elastography (RTE) in diagnosis of liver fibrosis. Methods:RTE was performed on 41 patients with chronic hepatitis B to measure the stage of liver fibrosis,from which a quantitative parameter-liver fibrosis index (LF index) was obtained. Simultaneously,biochemical test and liver biopsy were performed in all patients. We compared the results among different stages of S1,S2,S3 and S4 liver fibrosis reported by biopsy considered as the gold standard for the assessment of liver fibrosis. Results:Significantly different were found in LF index,APRI and FIB-4 of liver fibrosis groups(P﹤0.05). LF index,APRI and FIB-4 increased as the stage of liver fibrosis(P < 0.001). LF index correlated positively with APRI,FIB-4,respectively (P﹤0.01). Taking S4 as the diagnosis standard of early liver cirrhosis,the area under the curve of ROC for LF index,APRI and FIB-4 was 0.934(P < 0.001),0.851(P < 0.001),0.899(P < 0.001),respectively. Conclusion:RTE with potential practical value in the clinical has certain diagnostic efficiency in evaluating the stage of liver fibrosis.