Abstract:Objective:To investigate the role of TCP1-β in the proliferation of androgen-independent prostate cancer (AIPC). Methods:Androgen-dependent prostate cancer (ADPC) cell line LnCap and AIPC cell line PC3 were chosen as a model. Phenol-red free medium and FBS that was depleted of steroids by charcoal/dextran-treatment were used for the cell culture,to detect the androgen dependence of the cells. RNAi was used to inhibit the TCP1-β expression of PC3-Ad and LnCap cells. Q-PCR and Western blot were used to detect the expression of TCP1-β. The viability or apoptosis of PC3-Ad and LnCap cells was determined by MTT or flow cytometry(FCM) method. STRING was used to analyze the interaction between TCP1-β and AR. Results:The proliferation of LnCap but not PC3 cells was inhibited by androgen deletion. The expression of TCP1-β was significantly decreased when PC3-Ad and LnCap cells were transfected with siRNA of TCP1-β (P < 0.05). The proliferation of PC3-Ad but not LnCap cells was significantly inhibited when TCP1-β expression was inhibited (P = 0.002,P = 0.006). No significant apoptosis was detected in both PC3-Ad and LnCap cells when TCP1-β expression was inhibitied (P = 0.015). Conclusion:Over-expression of TCP1-β may involved into the regulation of AIPC proliferation.

Abstract:Objective:To construct the full-length NS1 gene of influenza A(H7N9) into an eukaryotic expression vector PXJ40-MYC,and study the expression of NS1 gene in transfected 293T cell. Methods:The NS1 gene of influenza A (H7N9) was amplified by RT-PCR and cloned into pMD18-T vector to construct a plasmid,named pMD18-T-NS1.The PCR product of pMD18-T-NS1 plasmid and the PXJ40-MYC were double digested using the same restrict enzymes,the recombinant eukaryotic expression vector PXJ40-MYC-NS1 was subsequently yielded. The expression of the NS1 gene in transfected 293T cells was tested by Western blot. Results:The recombinant eukaryotic expression vector PXJ40-MYC-NS1 was successfully constructed. The NS1 protein was finally expressed in 293T. Conclusion:The full-length NS1 gene was obtained as well as its recombinant eukaryotic expression plasmid was successfully constructed. The construction of eukaryotic expression plasmid of NS1 gene made it possible to further study the function of NS1 protein and the mechanism of diseases induced by influenza A virus.

Abstract:Objective:To construct eukaryotic expression vector pIRES2-EGFP-C5a,prokaryotic expression vector pET-21a-C5a of rat complement C5a containing histidine tag,and observe its expression and biological activity in vitro. Methods:Total RNA was isolated from rat liver cells, C5a gene was amplified by reverse transcriptional PCR and inserted into pIRES2-EGFP vector. After lipofectamine-mediated transfection of HEK293 cells with pIRES2-EGFP plasmid,the expression of rat C5a protein was determined by fluorescence microscope and Western blot. The prokaryotic expression vector of pET-21a-C5a was constructed,and the expression of C5a protein was determined by Western blot. Rat C5a was purified by Ni2+ chelating affinity chromatography column. The reactive oxidative species(ROS) generated from neutrophils which were stimulated by C5a was detected by flow analyzer. The mRNA of IL-6 and TNF-α was detected after rat GMCs were stimulated by C5a for different time. Results:The rat complement C5a gene was amplified by reverse transcriptional PCR successfully. The pIRES2-EGFP-C5a plasmid was successfully constructed and tranfected into HEK293 cells. The expression of green fluorescent protein was seen under by fluorescence microscopy,but no C5a was detected. Meantime,the pET-21a-C5a plasmid was also constructed,and the expression of C5a could be detected using Western blot. And the histidine tagged C5a protein was purified by Ni2+ chelating affinity chromatography column. C5a can stimulate the nertrophils to generate ROS. Conclusion:The pIRES2-EGFP-C5a plasmid and pET-21a-C5a plasmid were successfully constructed,and the expression of C5a in prokaryotic expression vector pET-21a-C5a could be detected and the product was biological active.

Abstract:Objective:To establish a culture method for primary mouse subcutaneous and visceral adipocyte precursor cells. Methods:Fibroblast-like cells were collected from C57BL/6J mice inguinal and epididymal adipose tissue. The morphological changes of the cultured cells were observed,the intracytoplasmic lipid of the culture cells was determined using oil red O staining,and the expression of the specific adipogenic gene was determined by real time PCR. Results:The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation and differentiate into mature adipocytes. Oil red O staining,morphological observation,and expression of specific adipogenic gene Fabp4 verified these cells as adipocyte. Conclusion:Adipocyte precursor cells are presented in C57BL/6J mice inguinal and epididymal adipose tissue and possess the potential to proliferate and differentiate into mature adipocyte. The establishment of primary culture of mouse subcutaneous and visceral adipocyte precursor cells is important for further studies of the differences between subcutaneous and visceral adipocyte in vitro.

Abstract:Objective:To establish a culture method for primary mesenchymal stem cells from neonatal mouse calvaria. Methods:Mesenchymal stem cells were collected from neonatal mouse calvaria with collagenase. The morphological changes and proliferation of the cultured cells were observed. Cell circle stages were analyzed by flow cytometry. The expression of cell-surface antigens were detected by flow cytometry. The potential of the isolated cells to differentiate into osteogenic and adipogenic lineages was examined. The mineralization and the intracytoplasmic lipid were detected using alizarin red staining and oil red O staining,respectively. Differentiation abilities were further validated by RT-PCR analysis of osteoblast specific gene and adipocyte specific genes. Results:The cultured spindle-like cells showed highly homogeneous appearance with active proliferation. The cells were positive for CD29 and CD44, while negative for CD34 and CD45. Visible mineralized nodules and lipid droplets were observed with alizarin red staining and oil red staining,respectively. In addition,the mRNA expression of osteoblast specific gene and adipocyte specific genes increased obviously after induction. Conclusion:Our research successfully established a culture method for primary mesenchymal stem cells from neonatal mouse calvaria. This method might be important for further studies of mesenchymal stem cells.

Abstract:Objective:To establish a culture method of cancer stem cells(CSCs) from esophageal cancer cell lines KYSE-150 and TE-1 by serum-free medium(SFM) and identify their characteristics. Methods:KYSE-150 and TE-1 cells were cultured in serum-free medium. The cell spheres were observed and cell differentiation was induced by serum supplement. MTT assay and invasive assay were applied to examine the invasive ability of both cell spheres and their parental cells. CD44+,CD271+,CD44+CD271+ tumor spheroid cells were also detected by flow cytometry. Results:After SFM culture,KYSE-150 and TE-1 cells formed spheres,and could be passaged continuously. The differentiation of cell spheres recurred when serum was supplemented. The proliferation and invasive ability of cell spheres were higher than their parents cells. CD44+,CD271+,CD44+CD271+ in KYSE-150 cell spheres were(64.92 ± 11.04)%,(28.27 ± 6.79)%,(24.07 ± 5.39)%,which were significantly higher than those in parents cells[(37.04 ± 6.30)%,(3.69 ± 0.49)%,(1.64 ± 0.11)%,P < 0.05]. CD44+,CD271+,CD44+CD271+ TE-1 cell spheres were (6.41 ± 0.87)%,(2.58 ± 0.17)%,(0.27 ± 0.53)%,which were also significantly higher than those in parents cells[(1.87 ± 0.18)%,(0.44 ± 0.10)%,(0.09 ± 0.03)%,P < 0.05]. Conclusion:Esophageal cancer cell spheres can be isolated from KYSE-150 and TE-1 by culture with SFM. CD44 and CD271 may be the cell surface makers for these cancer stem cells.

Abstract:Objective:To investigate in vivo and in vitro the role of PEDF on human lens epithelial cells biological behaviour regulation. Methods:In vivo study,aqueous PEDF concentration was detected by enzyme-linked immunosorbent assay(ELISA) in age-related cataract patients who underwent cataract phacoemulsification extraction surgery. The relationship of PEDF level was analyzed with LECs density on anterior lens capsular specimens and cataract types. In vitro study,PEDF was added in culture media of human lens epithelial cell line (HLE-B3). The growth activity of HLE-B3 was detected by Cell Counting Kit(CCK8). Flow cytometry with AnnexinV-FITC/7-AAD double staining method was used to detect the changes of cell cycle and apoptosis. Results:The mean aqueous PEDF concentration of 120 cataract cases was (156.2 ± 41.2)ng/ml. The central anterior subcapsular LEC density significantly decreased with aqueous PEDF level reducing(r = 0.556,P < 0.01),and the positive correlation remained after controlling the age factor(r = 0.387,P < 0.05). PEDF concentration in aqueous humor of cortical cataract was lower than that of nuclear sclerosis(P < 0.05). After adding PEDF in culture medium of HLE-B3 line,cell’s growth accelerated significantly(F = 187.33,P < 0.01),and apoptosis was significantly inhibited (the apoptosis rate fell from 13.50% to 2.08%,t = 7.90,P < 0.01),and the proliferative division activity increased (cell ratio in G2+S phase rose from 28.54% to 54.05%,t = 6.32,P < 0.01). Conclusion:In human eyes,PEDF may function as LEC cytotrophic factor to play the important biological effects of cell cycle regulation and anti-apoptosis,involved in maintenance of lenticular physiological state and cataract development.

Abstract:Objective:To investigate the effects of new tumor necrosis factor-α-inducing protein (Tip-α) on the expression of cytokines and its significance. Methods:Tip-α were amplified from genome sequence of Helicobacter pylori (H.pylori) strain 26695. The recombinant plasmid was transformed into E.coli. The expression product was purified and confirmed by Western blot. After treated by different concentrations of Tip-α in different times,the level of cytokines(IL-8,TNF-α,IL-1β) in GES-1 were detected by ELISA. They were also detected after NF-κB was inhibited by pyrrolidine dithioearbamate (PDTC,the special inhibitor of NF-κB). Results:It was confirmed by Western blot analysis that Tip-α antibody combined with purified proteins specifically. When the cells were treated with Tip-α,the levels of cytokines significant increased(P < 0.05). After NF-κB was inhibited by PDTC,the levels of cytokines significant decreased (P < 0.05). Conclusion:Tip-α can promote the expression of cytokines(IL-8,TNF-α,IL-1β),it may play an important role in the phlogogenic effect of HP,this effect may be NF-κB signal pathway dependent.

Abstract:Objective:To detect the expression and explored the role of ROCK1 in hepatocellular carcinoma (HCC). Methods:The expression of ROCK1 mRNA in HCC samples and cell lines was detected by real-time PCR,while the expression of ROCK1 protein in HCC tissues and adjacent non-cancer tissues was assessed by immunohistochemistry. In addition,invasion of HCC cells was observed after overexpressing or silencing ROCK1. Results:In the total of 60 paired HCC specimens,compared with the adjacent non-cancer tissues,the expression of ROCK1 mRNA was up-regulated in 42 cases(P < 0.05),and the expression of ROCK1 protein in HCC tissues was higher than that in adjacent non-cancer tissues. Overexpression of ROCK1 enhanced HCC-derived HepG2 cellular invasion in vitro. In contrast,ROCK1 knockdown via RNAi markedly suppressed these phenotypes in SMMC-7721. Conclusion:This study indicates ROCK1 is frequently overexpressed in hepatocellular carcinoma,and contributes to tumor cell invasion. ROCK1 may be a novel therapeutic target for prevention and treatment of invasion in HCC.

Abstract:Objective:To investigate the role of heparanase(HPA) gene silencing by miRNA interference on invasion and migration of SACC-M cells. Methods:Expression vectors of recombinant plasmids expressing artificial HPA miRNA were constructed and transfected to knockdown HPA expression in SACC-M cells. Expressions of HPA mRNA and protein were detected by RT-PCR and Western blot,respectively. Matrigel invasion assay and scratch-wound assay were performed to examine the invasive and migration ability of the SACC-M cells. Results:Transfection of recombinant plasmid of pGPH1/GFP/Neo/HPA-siRNA significantly suppressed the HPA mRNA and protein levels. This knockdown significantly inhibited the invasion and migration of SACC-M cells as compared with controls. Conclusion:HPA may play an important role in invasion and migration in human salivary adenoid cystic carcinoma cells and should be considered as a potential small molecule inhibitor therapeutic targeting strategy for the future.

Abstract:Objective:To explore the effects of borneol combined with cisplatin (DDP) on cell proliferation and cell cycle and apoptosis of human esophageal carcinoma cell line Eca109(DDPr). Methods:The human esophageal carcinoma cell line Eca109 was exposed to continuous stepwise-increased concentration of DDP. Drug-sensitivity was measured by MTT. The cell cycle and apoptosis were determined by flow cytometry. Results:After 6 months of stepwise drug exposure,the cell could normally grow and propagate in the medium with 1 μg/ml of DDP,and exhibited a resistance index of 15.886 against DDP. The cell line was named Eca109(DDPr). MTT assay showed that borneol at the concentration of no more than 1.560 μg/ml had no obvious inhibition on the growth of Eca109(DDPr) cells(P > 0.05),but it could significantly promote the inhibition of DDP(1 μg/ml) on the proliferation of Eca109(DDPr) cells(P < 0.05). Compared with using DDP alone,borneol combined with DDP could change the cell cycle,significantly increase the number of cells in G1 stage(P < 0.05) and decrease the number of cells in S stage and G2 stage(P < 0.05),and obviously promote the apoptosis of Eca109(DDPr) cells(P < 0.05). Conclusion:Borneol can promote the inhibition of DDP on the proliferation of Eca109(DDPr) cells,the mechanism may be related to change cell cycle and increase apoptosis of Eca109(DDPr) cells.

Abstract:Objective:To investigate the effect of triptolide on the invasion of human endometrial carcinoma cell line (HEC-1B). Methods:Gelatin zymography was performed to investigate the effects of triptolide on the activity of the invasion-related gelatinase proteins MMP-2 and MMP-9 in HEC-1B cells. Transwell was employed to assess the influence of triptolide on the invasion of HEC-1B cells. Results:The activity of the invasion-related gelatinase proteins MMP-2 and MMP-9 and the invasion of HEC-1B cells were decreased in a time-dependent manner,when exposed to triptolide. Conclusion:Triptolide could inhibit the invasion of HEC-1B by down-regulating the activity of the invasion-related gelatinase proteins MMP-2 and MMP-9.

Abstract:Objective:To observe the protective effect of methanesulfonic acid cinepazideon(CMI) on cerebral ischemia reperfusion injury in rats. Methods:The local cerebral ischemia/reperfusion model was established by intraluminal thread occlusion of the middle cerebral artery occlusion (MCAO) in rats. Seventy-two male SD rats were randomly divided into five groups:model group,sham group,CMI treatment group (20,40,80 mg/kg) and cinepazide maleate treatment group (40 mg/kg). Thrombosis,platelet aggregation,the infraction volume and pathomorphological changes of brain tissue were observed in each group. Results:Compared with model group,CMI could reduce infract volume,thrombosis and inhibit platelet aggregation (P < 0.05). Also it could reduce neurons karyopyknosis,dissolution and swelling of the brain cells. And the effects had no difference with cinepazide maleate. Conclusion:CMI has a marked neuroprotective effect on local cerebral ischemia reperfusion injury in rats.

Abstract:Objective:To estimate the possible effect of placental monocyte-macrophages on the pregnant immunological tolerance by observing the expression of indoleamine 2,3-dioxygenase(IDO) and the secretion of IL-10 and IL-12 in cells from different gestational phages. Methods:Monocyte-macrophages cells were separated mechanically from normal term (39 weeks ± 5 days,25 cases) and midtrimester (21 weeks ± 3 days,19 cases) placental tissues. The cytokines including IL-12 and IL-10 were assayed by ELISA,while the expression of IDO was measured by RT-PCR and Western blot,respectively. The monocyte-macrophages cells derived from placental tissue in different gestational phages were co-cultured with allogeneic T cells in order to detect their abilities in stimulating the proliferation of allogeneic T cells detected by CCK-8. Results:Monocyte-macrophages cells from normal term placental tissues secreted high level of IL-12,but expressed low levels of IL-10 and IDO. Besides,monocyte-macrophages cells from normal term placental tissues were more powerful in promoting the proliferation of allogeneic T cells than the cells from midtrimester term placental tissues. Conclusion:Monocyte-macrophages cells from normal term and midtrimester placental tissues may play roles in the maintenance of pregnant immunological tolerance and delivery possibly by placental tissue in different gestational phages possess different biological characters,which possibly by regulating the IL-12/IL-10 balance and IDO expression. However,the association of IL-12/IL-10 balance with the expression of IDO needs further elucidation.

Abstract:Objective:To determine whether the protein expression of ribonucleotide reductase subunit 1(RRM1) and breast cancer susceptibility gene 1(BRCA1) in tumor tissue could predict the efficacy of gemcitabine combined with cisplatin (GP) on stage ⅢB/Ⅳsquaomas lung cancer. Methods:A total of 89 stage ⅢB/Ⅳsquaomas lung cancer patients were included,only 78 patients were evaluated. Bronchoscopy or lung puncture tumor biopsies samples were obtained and RRM1,BRCA1 protein expression were examined immunohistochemically before chemotherapy,then the patients were randomly assigned to received 4 cycles of GP chemotherapy regiments. Follow-up was done afterwards until the patient died or could not be informed. Response rate (RR),overall survival (OS) and time to tumor progression (TTP) were assessed. Results:Among 78 evaluated patients,the positive expression rates of RRM1 and BRCA1 were 47.4%(37/78) and 51.3%(40/78),respectively. There was no significant difference between positive expression rates and patients’ clinical characteristics. According to the different levels of RRM1 and BRCA1,the patients were divided into four groups:group A (low expression of both RRM1 and BRCA1),group B (high expression of both RRM1 and BRCA1),group C (high expression of only RRM1) and group D (high expression of only BRCA1). The RR was higher in group A than in other three groups (group A 59.1%,group B 38.1%,group C 42.1 %,group D 43.8%). The OS and TTP were longer in group A than in other three groups[OS:group A(617.4 ± 19.6)days,group B(299.2 ± 20.5)days,group C (336.6 ± 22.7)days,group D(324.7 ± 24.2)days;TTP:group A(274.9 ± 21.8)days,group B(138.8 ± 18.0)days, group C(172.5 ± 17.3)days, group D(167.6 ± 19.8)days]. There were no significant difference among group B,C,and D. Conclusion:The expression level of RRM1 and BRCA1 in sequomas lung cancer is probably predictive factor of the efficacy of GP chemotherapy regiments. It is more suitable for squaomas lung cancer patients who expressing lower RRM1 and BRCA1 to adopt GP chemotherapy regiments.

Abstract:Objective:To study the expression and significance of COX-2 in triple-negative breast carcinoma(TNBC) and explore the relevance of COX-2 and neovascularization(MVD) marked by CD31. Methods:The expression of COX-2,MVD in 60 TNBC cases and 518 paraffin wax samples of non-TNBC patients were detected by immuno-histochemical method,and the clinical characteristics of TNBC cases and non-TNBC cases were compared. Results:①Compared with non-TNBC patients,TNBC cases were younger and mostly premenopausal patients,the differentiation of TNBC was lower while the percentage of histology graded Ⅲ was significantly increased;②The positive rate of COX-2 in 60 cases of TNBC was 73.3%,and that in 518 cases of the non-TNBC was 58.0%. The difference is statistically significant(P < 0.05);③In 60 cases of TNBC,the value of MVD(CD31 marked)was 183.73 ± 25.64,in 518 cases of non-TNBC,MVD was 168.84 ± 24.07(P < 0.01);④The expression of COX-2 in TNBC was significantly correlated with CD31(P < 0.01). Conclusion:The high expression of COX-2 and MVD are closely associated with infiltration and metastasis of TNBC,thus the abnormal expression of COX-2 and MVD are likely to participate together in the genesis and progress of TNBC.

Abstract:Objective:To explore the efficacy of neoadjuvant chemotherapy in the treatment of advanced gastric cancer. Methods:From October 2008 to March 2011,58 operable patients with stageⅡ~Ⅲc gastric cancer received DOX as neoadjuvant chemotherapy before surgery,and 58 cases of advanced gastric cancer that were not treated with neoadjuvant chemotherapy at the corresponding time period were designated as the controls and underwent surgery within two weeks. According to status of the patients and the principles of standard treatment for gastric cancer,they received postoperative chemotherapy. Results:In the chemotherapy group,there were 5 CR cases,36 PR cases,16 SD cases,and 1 PD case. The total effective rate was 63.8%. The R0 resection rate was significantly higher than that in the control group(87.9% vs 63.8%,P < 0.05). The effective rate of esophagogastric cancer(EGC) was higher than the distal gastric cancer(83.3% vs. 55.0%,P < 0.05). The most common side effects were granulocytopenia,gastrointestinal tract adverse reaction,and periphery neurotoxicity. There was no significant difference in the incidence of postoperative complications(3.4% vs 1.7%)between the two groups. The one-year survival rates of the two groups were both 100%. The two-year survival rate in the chemotherapy group was significantly higher than that in the control group(75.8% vs 55.1%,P < 0.05). Conclusion:The efficacy of DOX as neoadjuvant chemotherapy in the treatment of advanced gastric cancer especially EGC can reduce the tumor size and tumor stage as well as improve R0 resection rate and overall survival.

Abstract:Objective:To investigate the characteristics of lymph nodes metastasis in T1 non-small cell lung cancer(NSCLC).Methods:The clinical data of 276 cases underwent resection of NSCLC from January 2011 to November 2012 were analyzed retrospectively. Characteristics of tumors,lymph nodes involvement were analyzed in those cases. Results:①In all 276 cases,66 cases were confirmed pathology with lymph node metastasis,metastasis rate was 23.9%(66/276). The metastatic rates in group of well differentiated and poorly differentiated were 6.1% and 33.7% respectively,the difference being significant(P < 0.05). ② The frequency of metastasis was higher in patients with a primary tumor in the right middle lobe compared to the right upper and left lower lobe(χ2 = 5.545,P < 0.05 and χ2 = 5.402,P < 0.05). Conclusion:In T1 NSCLC,lymph nodes metastatic rate is closely related with the degree of tumor differentiation. The frequency of metastasis was higher in patients with a primary tumor in the right middle lobe. It is necessary to perform systematic node dissection during pulmonary resection for those patients.

Abstract:Objective:To investigate the application of the Addenbrooke’s cognitive examination-revised(ACE-R) to the assessment of global cognition in Parkinson’s disease(PD). Methods:Twenty-four PD patients were included. Their cognitive functions were assessed according to the ACE-R scales. Results:Compared with 11 cases of abnormal MMSE scores and 13 cases of normal MMSE scores in 24 cases of PD patients,the age was significantly different in 2 groups with MMSE(P < 0.05),but the duration of symptoms,Hoehn-Yahr stage and UPDRS-motor were not significantly different. Compared with 14 cases of abnormal ACE-R scores and 10 cases of normal ACE-R scores in 24 cases of PD patients,the duration of symptoms,Hoehn-Yahr stage and UPDRS-motor were significantly different in 2 groups with ACE-R (P < 0.05),but the age was not significantly different. The scores of five cognitive subdomains in ACE-R(attention and concentration,memory,verbal fluency,language and visuospatial) in 2 groups with ACE-R were significantly different(P < 0.005). Conclusion:The ACE-R has higher sensitivity than the MMSE for cognitive assessment in PD patients. The ACE-R is more suitable than the MMSE as a cognitive screening tool for PD patients.

Abstract:Objective:To study the genetic polymorphism,frequencies and genetic distance of 24 Y-chromosomal short tandom repeat(STR) loci in unrelated individuals of Han nationality in Jiangsu province. Methods:Twenty-four STR loci were amplified in DNA samples from 262 unrelated individuals in Jiangsu Han population from 2011 to 2012. All samples of the PCR products were genotyped by ABI 3130 Genetic Analyzer. Results:In total,172 genotypes and 262 haplotypes were founded,DYS385b,DYS449 loci had 14 genotypes,the diversity of overall heliotypes for the 24 Y-STR loci was 0.999 9. Comparing with 9 other populations,the genetic distance was more closed between Jiangsu and Tianjin (-0.001 8),Henan (-0.001 76),Shanxi (-0.001 6) province,compared to Hunan(0.017 54) and Liaoning(0.014 64) population(P < 0.05). Conclusion:We obtained objective basic data in the area of population genetics and forensic medicine,which would be useful for building Y-STR database in Jiangsu Han population.

Abstract:Objective:To evaluate the association of 25-hydroxyvitamin D3[25(OH)D3]and zinc transporter 8 antibodies(ZnT8A) in newly diagnosed type 1 diabetes mellitus(T1DM). Methods:There were 32 newly diagnosed type 1 diabetes mellitus and 100 controls in this study. Clinical data were collected,biochemical parameters,serum 25 (OH)D3 levels and ZnT8A were detected;the relevance of serum 25(OH)D3 and ZnT8A were analyzed. Results:①Serum 25(OH)D3 level was significantly lower in T1DM than that in the controls(P < 0.01). In the vitamin D deficiency group,the prevalence of T1DM was increased,risk odds ratio (OR = 3.16,95% confidence region:3.02~3.31).②From vitamin D sufficient group to vitamin D deficient group,fasting and postprandial C-peptide level were gradually decreased(P < 0.05);the ZnT8A positive rate in vitamin D deficient group was higher than vitamin D sufficient group (P < 0.05).③The ZnT8A positive rate was negatively related with serum 25(OH)D3 concentrations(r = -1.036,P < 0.05). Conclusion:①The serum 25(OH)D3 level in T1DM was significantly lower than that in the controls. The T1DM patients are at risk for vitamin D insufficiencies. ②There was interaction between the serum 25(OH)D3 concentration and ZnT8A.

Abstract:Objective:To observe the differences between serum chemotactic factors and oxidative stress markers in different periods of diabetic nephropathy(DN) and the changes of then after therapy of α-lipoic acid. To explore the correlation of chemotactic factors and oxidative stress markers in diabetic nephropathy and the effects of α-lipoic acid to them. Methods:One hundred patients with type 2 diabetic mellitus were randomly divided into normal group,early DN group and clinical DN group according to 24 hour urinary albumin(24h-UAL). Changes of FKN,MCP-1,GSH-PX,SOD,MDA,24h-UAL,FPG,2hPG,HbA1c and 24h-UAL before and after treatment of α-lipoic acid in three groups were observed. Thirty healthy person were chosen as control group. Results:①Serum GSH-PX and SOD in diabetic groups were significatly lower and serum chemotactic factors and MDA were higher than control group. Serum chemotactic factors and MDA decreased,while GSH-PX and SOD increased significantly after treatment in all groups, 24h-UAL was lower than before in early DN group and clinical DN group. Levels of FKN,MCP-1 and MDA in clinical DN group were obviously higher while GSH-PX and SOD were lower than those in normal two group and early DN group before and after treatment, (P < 0.05). ②Serum chemotactic factors were positivly correlated with MDA,24h-UAL,FPG,2hPG and HbA1c,and negativly correlatied with GSH-PX and SOD. MDA was positivly correlated with 24h-UAL,FPG,2hPG and HbA1c,while GSH-PX and SOD were opposite. Conclusion:Serum chemotactic factors and markers of oxidative stress significantly correlat with 24h-UAL and serum glucose. Serum chemotactic and urine protein are decreased by antioxidant,which may delay the progression of diabetic nephropathy.

Abstract:Objective:To explore the risk factors of patients with type 2 diabetes mellitus (T2DM ) with non-alcoholic cirrhosis (NAFLD). Methods:The enzyme-linked immunosorbent assay (ELISA) and chemiluminescene analyzer were used to detect the serum cytokeratin 18(CK18),free fatty acids (FFA),tumor necrosis factor alpha (TNF-α),superoxide dismutase enzyme (SOD) in 45 cases of T2DM,44 cases of T2DM+NAFLD patients and 24 cases of control. Body mass index(BMI),insulin resistance index(HOMA-IR),fasting plasma glucose (FPG) and general physiological and biochemical indexes of the study group and the control group were simultaneously detected and calculated,and their correlation with NAFLD was analyzed by correlation analysis and binary logistic regression statistical analysis. Results:BMI,HOMA-IR,FPG,TC,TG,AST,ALT,GGT,FFA,CK18 and TNF-α were positively related with NAFLD. SOD was negatively related with NAFLD. FINS and HbA1c have no relation with NAFLD. BMI,HOMA-IR,FFA and CK18 were highly related with etiology of NAFLD. Conclusion:The pathogenesis of NAFLD is the result of multiple factors. The BMI,HOMA-IR,FFA and CK18 is independent risk factor of the NAFLD.

Abstract:Objective:To evaluate the value of related characteristic of CT and MR images in differentiating malignant from benign orbital tumors. Methods:Seventy-nine cases with orbital lesions confirmed by CT,MR and pathological examination were enrolled in this study. The tumors,side,size,shape,margin,location,number of affected quadrant,bone destruction,T1 and T2 signal,homogeneity of T1 and T2 signal,enhancement pattern,and CT value were determined. Using single variate analysis and multivariate logistic regression,the correlation between the above mentioned factors and benign or malignant tumors was analyzed. Results:Seventy-nine cases included 49 benign cases and 30 malignant ones. Single variate analysis indicated that tumors,shape,margin,location,bone destruction,and T2 signal bore a close relationship to the benign and malignant orbital tumors. The multivariate logistic regression analysis showed that bone destruction and iso-intense on T2 weighted image were the risk factors of malignant orbital tumors. Conclusion:CT and MR can accurately locate the tumors in the orbit. If combined with bone destruction or iso-intense on T2 weighted image,the orbital lesions might be strongly suspected to be malignant .

Abstract:Objective:Based on identification of anatomic landmarks of lateral ventricles, the study aims to measure the volumes and angles of head,body and tail of lateral ventricles in young,middle-aged and old people. Methods:Sixty healthy adults(divided equally into young group,middle-aged group and the elderly group by age) were scanned with T2-MR to determine the anatomic landmarks of lateral ventricle. Then the volumes,angles and numbers of scanning layers were measured in the three groups. The data were analyzed by one-way ANOVA. A lateral ventricle model was three-dimensional reconstructed. Results:All of the anatomic landmarks of lateral ventricles in subjects were distinguished clearly,such as the corpus callosum,septum pellucidum,fornix,head of caudate nucleus and membrane network bifida. The differences of the bilateral anterior horn,body and posterior horn of lateral ventricle and overall volume between the young group and the elderly group were statistically significant(all P < 0.05). The volumes of lateral ventricle between middle-aged group and the young group had no significant differences except the anterior horn of lateral ventricle(P < 0.05). The differences of the bilateral anterior horn,posterior horn of lateral ventricle and overall volume,left and right angles of anterior horn and width of temporal horn of lateral ventricle between the middle-aged group and the elderly group were significant (all P < 0.05). Conclusion:According to the principles to confirm the anatomic landmarks of the lateral ventricle,the study provides a standardized form for measurements of volume and angle of lateral ventricle,which may be helpful for the diagnosis and research of the pathogenesis.

Abstract:Objective:To analysis the value of single-photon emission tomography/computed tomography(SPECT/CT ) in diagnosing parathyroid in patients with hyperparathyroidism. Methods:Thirty-five patients with secondary hyperparathyroidism (SHPT) were performed 99mTc-methoxyisobutylisonitrile(99mTc-MIBI) dual-phase planar scintigraphy (MIBI planar),and 46 focis outside of thyroid-bed were observed. SPECT/CT imaging including neck and additional radical uptake was added in them. The difference in sensitivity and false positive percent(FPP) between MIBI planar and SPECT/CT in finding the normalsite parathyroid and ectopic parathyroid were compared according to the pathology. Results:The sensitivity of MIBI planar in finding the normalsite parathyroid was similar to SPECT/CT(63.9% vs 67.0%,P > 0.05),the FPP was significantly lower than SPECT/CT(3.0% vs 18.4%,P <0.05). The sensitivity of MIBI planar in finding the ectopic parathyroid was similar to SPECT/CT(either 92.5%),the FPP was significantly lower than SPECT/CT(2.6% vs 19.6%,P < 0.05). The sensitivity of SPECT/CT in finding the ectopic parathyroid was significantly higher than itself in finding the normalsite parathyroid(92.5% vs 67.0%,P < 0.05). Conclusion:SPECT/CT can not add the sensitivity in finding parathyroid,but can significantly reduce FPP. It is very valuable in clinical work,especially in finding the ectopic parathyroid.

Abstract:Objective:To estimate the association between the XRCC3 Thr241Met polymorphism and hepatocellular carcinoma (HCC) risk. Methods:We performed a comprehensive search of the PubMed,EMBASE,Wanfang,China National Knowledge Infrastructure (CNKI) and Weipu databases for studies on the relationship of the XRCC3 Thr241Met polymorphism with HCC risk. The pooled odds ratios (ORs) with the corresponding 95% confidence intervals (95%CIs) were calculated in this meta-analysis. In addition,subgroup analyses by source of populations,HBV infection and smoking status were conducted for further estimation. The source of between-study heterogeneity was also evaluated. Results:Five eligible papers published from 2008 to 2012 with 1 741 HCC cases and 2 596 controls were finally included into the present meta-analysis. ① Meta-analysis of total included studies in Chinese population showed that the XRCC3 Thr241Met polymorphism was significantly associated with an increased risk of HCC in all genetic contrast models (ORT vs C = 1.84,95%CI:1.19~2.85,POR = 0.003;ORTT vs CC = 4.71,95%CI:2.14~10.34,POR < 0.001;ORCT vs CC = 1.59,95%CI:1.07~2.36,POR = 0.022;ORTT vs CC+CT = 4.21,95%CI:2.21~8.00,POR < 0.001;ORCT+TT vs CC =1.83,95%CI:1.11~3.00,POR = 0.017). ② Meta-analysis in Guangxi population suggested that individuals carrying the variants of XRCC3 Thr241Met were more susceptible to hepatocellular carcinoma (ORT vs C = 2.23,95%CI:1.32~3.77,POR = 0.003;ORTT vs CC=5.74,95%CI:2.33~14.14,POR < 0.001;ORCT vs CC = 1.91,95%CI:1.23~2.99,POR = 0.004;ORTT vs CC+CT = 4.63,95%CI:2.20~9.76,POR < 0.001;ORCT+TT vs CC =2.29,95%CI:1.26~4.18,POR = 0.007). ③ In subgroup analyses by HBV infection and smoking status,no associations were found between the HBV infection and smoking status and HCC risk. Conclusion:The present meta-analysis suggested an important role of the XRCC3 Thr241Me polymorphism in the risk of developing HCC in Chinese,especially in Guangxi population,but no interactions of gene-environment were observed.