Abstract:Objective:To explore the underlying molecular mechanisms of exogenous bisphenol A(BPA) regulating MCF-7 breast cancer cell migration. Methods: The migration of MCF-7 breast cancer cells was detected by wound healing assay and transwell assay, and further confirmed by adhesion assay. Furthermore, the migratory ratio of MCF-7 breast cancer cells was analyzed by knockdown of Snail. Results: Exogenous BPA was able to increase the expression of Snail in MCF-7 breast cancer cells and decrease the expression of E-cadherin and thus promote cell migration. Knockdown of Snail was able to retard the MCF-7 breast cancer cell migration. Furthermere,BPA stimulation reduced the adhesion ability of MCF-7 breast cancer cells. Conclusion: We demonstrated that exogenous BPA induced MCF-7 breast cancer cell migration by upregulating Snail protein expression. These findings provide a new clue for better understanding the mechanism of breast carcinoma metastasis.

Abstract:Objective:To validate the effect of hypertension on small conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats. Methods: Male spontaneously hypertensive rats (SHR) (n = 8) and normotensive Wistar Kyoto rats (WKY) (n = 8) were selected. A series of electric stimulation were utilized to the corpus cavernosum nerves of rats, the changes of ICP /MAP were observed repeatedly, and the expressions of SKCa protein and mRNA were assessed by Western blotting and RT-PCR. Results: Compared with the WKY group, ICP /MAP and the expression of SKCa gene mRNA and protein were significantly lower in the SHR group (P < 0.05), and the expression of SKCa protein and mRNA was significantly lower in the SHR group in comparison with that of the WKY group (P < 0.05). Conclusion: Erectile dysfunction in hypertension is associated with the low expression of SKCa in the cavernous tissue of spontaneously hypertensive rats.

Abstract:Objective:To explore whether hypoxia-inducible factor-1α(HIF-1α)mediates hypoxic proliferation in airway smooth muscle cells(ASMC). Methods:Rat ASMC were incubated in vivo and grouped in three:ASMC that is cultured under normoxia was defined as control group(A group),ASMC that is cultured under hypoxia was defined as pure hypoxia group(group B),ASMC that is cultured under hypoxia and pretreated with 2-methoxyestradiol(2ME),which is inhibitor of HIF-1α,was defined as hypoxia and 2ME pretreatment group(group C). Proliferation of ASMC was evaluated by MTS. Real-time PCR and Western blot were used to analyze expression of HIF-1α mRNA and protein. Results:①The proliferation of rats ASMC is significantly higher in group B than that in group A(1.123 ± 0.006 vs 0.809 ± 0.003,P < 0.05),but it is significantly lower in group C than that in group B(1.012 ± 0.005 vs 1.123 ± 0.006,P < 0.05). ②Expression of HIF-1α mRNA and protein increased significantly in group B than that in group A(5.265 ± 0.040 vs 0.449 ± 0.017,P < 0.05 and 0.905 ± 0.013 vs 0.105 ± 0.008,P < 0.05),and decreased significantly in group C than that in group B(1.903 ± 0.044 vs 5.265 ± 0.040,P < 0.05 and 0.278 ± 0.012 vs 0.905 ± 0.013,P < 0.05),expression of HIF-1α mRNA(r = 0.850)and protein(r = 0.883) in ASMC correlated significantly with proliferation. Conclusion:HIF-1α mediated rat ASMC proliferation under hypoxia.

Abstract:Objective:The aim of the study was to evaluate the growth differentiation factor-15(GDF-15) level in the reduced uterine perfusion pressure(RUPP) rat model by using uterine placenta ischemia to induce gestational hypertension in rats. Methods:A total of 20 Spragu-Dawley rats were randomly divided into two groups with each group of 10 rats:the sham group and the RUPP group. The operation was taken on the 14th day after gestation. On the 13th and 19th day gestation,the blood pressure was measured. The blood was collected on the 19th day gestation for the GDF-15 test. Results:In the RUPP rats,both the systolic pressure and diastolic pressure increased significantly on the 19th day gestation compared with that on the 13th gestation[(157.8 ± 9.0)mmHg vs (118.3 ± 5.7)mmHg,(122.5 ± 6.9)mmHg vs (88.4 ± 8.0)mmHg,P < 0.05],and the sham group had no significant changes. Compared with the sham group on 19th days gestation, RUPP group rats were characterized by higher systolic pressure[(157.8 ± 9.0)mmHg vs (117.0 ± 9.4)mmHg;P < 0.05],higher diastolic pressure[(122.5 ± 6.9)mmHg vs (100.7 ± 4.4)mmHg;P < 0.05]. In the RUPP group,the GDF-15 level was significantly higher than that in the sham group[(363.3 ± 78.1)ng/L vs (276.7 ± 69.4)ng/L;P < 0.05]. Conclusion:GDF-15 level increased significantly in the gestation hypertension rats and is a potential cardiovascular prognostic maker for the gestation hypertension patients.

Abstract:Objective:To explore the effects of Y-box binding protein 1(YB-1) on the proliferation and transforming growth factor-β1 (TGF-β1) secretion in rat mesangial cells (MC). Methods:Rat mesangial cells were cultured with or without YB-1 over-expression,and the supernatant and the cells were collected respectively. The proliferation of MC with YB-1 over-expression was determined by MTT and cell counting. The level of the TGF-β1 protein in the supernatant was mesured by ELISA,while the TGF-β1 mRNA expression was detected by real-time PCR. Results:YB-1 over-expression stimulated the proliferation of MC,upregulated the expression of TGF-β1 mRNA,and induced the TGF-β1 protein secreting in rat MC. Conclusion:YB-1 triggered MC proliferation and increased TGF-β1 secreting,and inhibiting YB-1 expression may be beneficial in retarding progressive development of glomerulonephritis.

Abstract:Objective:To investigate the role of G protein coupled receptor kinase 2 (GRK2) interacting protein-1 (GIT1) in bone fracture healing. Methods:Femur fractures in GIT1 knockout (GIT1KO) mice (n=30) and WT mice (n=30) were performed. X-ray,micro-computed tomography imaging (micro-CT),immunofluorescence of PECAM1 were performed 7,14 and 21 days after fracture. Results:Fracture healing was delayed and blood vessel volume as well as number were decreased in GIT1KO mice compared to those in WT controls. Conclusion:GIT1 is an important signaling participant in fracture healing,with gene ablationleading to reduced callus vascularity and reduced osteoclast number in the healing callus.

Abstract:Objective:To study the effects of mixed-lineage kinase 3 (MLK3) signaling pathway on neuron from hippocampal CA3 region in epilepsy rats and ischemia rats. Methods:Seizure models and cerebral ischemia models were induced by kainic acid (KA) and four-vessel occlusion in SD rats,respectively. Immunoblotting was performed to examine the activation of MLK3 and c-Jun N-terminal kinase (JNK) at different times after KA injection and ischemia-reperfusion (I/R) in hippocampal CA3 regions,respectively. The neuronal survival in hippocampal CA3 regions was observed by cresyl violet staining both in epilepsy rats and ischemia rats. Results:The phosphorylation of MLK3 and JNK in cytoplasm increased rapidly at 6 h after KA injection than those in the saline group,and still was in higher level at 1 d in hippocampal CA3 region (P < 0.05). Total protein expression of MLK3 and JNK showed no significant change. However,the expression of p-MLK3 and p-JNK showed no obvious changes at different time after reperfusion in hippocampal CA3 region. Furthermore,a large number of neurons were loss in hippocampal CA3 region at 7 d after KA injection. Correspondingly,no obvious neuronal damages were found in hippocampal CA3 regions at 7 d after reperfusion. Conclusion:MLK3/JNK signaling pathway plays an important role in neuronal cells death in hippocampal CA3 region in epilepsy rats,however,MLK3/JNK pathway does not mediate the neuronal survival in hippocampal CA3 region in ischemia rats.

Abstract:Objective:To explore effects of carnosine on peripheral blood T-lymphocyte immunity in MCAO rats and the neuroprotective mechanisms. Methods: Thirty-six male SD rats were randomly divided into three groups: the sham-operated group (n=12), the model group (n=12), and the carnosine-treated group (n=12). The middle cerebral artery occlusion model was established by suture method in the model group and the carnosine-treated group. After establishment of MCAO model, the rats in the carnosine-treated group were lavaged with soluble canosine once a day at the dose of 1 000 mg/kg, and the same volume of normal saline was lavaged in the sham operation and model group. The neurological function was scored with Longa 5-point scale at baseline, 24 h and 72 h. The level of peripheral blood T-lymphocyte subset (CD3+, CD4+, CD8+) was detected by flow cytometry at 24 and 72 h, and calculate CD3+/CD8+ ratio. The infarction volume was revealed by TTC stain at 72 h. Results: The infarction volumn was detected in both of the model group and the camosine-treated group. Compared with the model group, the infarction volume was smaller (P < 0.05); Compared with the model group, there was no significant different in the neurological outcome scores in the carnosine-treated group at baseline and 24 h (P > 0.05); however, there was a significant decrease at 72 h of the carnosine-treated group (P < 0.05); Compared with the sham-operated group, the level of CD3+, CD4+ and the CD4+/CD8+ ratio was significant decreased (P < 0.05) and the level of CD8+ was increased in the model group at 24 and 72 h (P < 0.05). Compared with the model group, the level of CD8+ was significant decreased in the carnosine-treated group at 72 h (P < 0.05). Conclusions: Carnosine could has potential protective effective on peripheral immune suppression of MCAO rats and the mechanism could be CD3+, CD4+/CD8+ elevation and CD8+ inhibition.

Abstract:Objective:To detect the effect of insulin on apoptosis of cultured human umbilical vein endothelial cells (HUVECs) induced by hyperglycemia and the expression of caveolin-1 in HUVECs. Methods:HUVECs were cultured and randomized into group A(the controlled group,glucose concentration 5.5 mmol/L),group B (the hyperglucose group,glucose concentration 33.3 mmol/L),group C(the hyperglucose and low concentration of insulin group,glucose concentration 33.3 mmol/L+insulin concentration 20 mU/L),group D (the hyperglucose and high concentration of insulin group,glucose concentration 33.3 mmol/L+insulin concentration 1 000 mU/L). Flow cytometry was applied to determine cell apoptosis ratio and cell cycle. Cav-1 expression of HUVEC was detected by immunohistochemistry analysis. Results:High concentration of glucose induced sighificantly increased apoptosis of HUVEC which was associated with the arrest of the cell cycle of G0/G1, and the expression of cav-1 was decreased. Low concentration of insulin reduced the apoptosis of HUVEC and increased cav-1 expression. While high concentration of insulin had no effects on the cells apoptosis,cell cycle and cav-1 expression. Conclusions:Low concentration of insulin inhibited the apoptosis induced by hyperglycemia,while high concentration of insulin had no effects on the apoptosis induced by hyperglycemia, and its mechanism may be related to the expression of cav-1.

Abstract:Objective:To investigate the effects of vitamin A on appetite and the gene expression of MC4R, POMC, and to explore the mechanism of vitamin A on rats’ appetite. Methods: Male Sprgue-Dawley (SD) rats were divided into two groups: group A (the vitamin A deficient group) and group B (the vitamin A normal control group). Seventy-four days later, 16 rats were selected from group A and then divided into two groups: group A1 (the vitamin A deficient group) and group A2 (the vitamin A administration group). Also 8 rats were selected from group B as group B1 (the vitamin A normal control group). At the 79th day, all the rats in the three groups were sacrificed, and some relevant indices were measured. The remaining rats in group A were divided into two groups again: group A3 (the vitamin A deficient group) and group A4 (the vitamin A supplement group). The remaining 8 rats in group B was group B2 (the vitamin A normal control group). All the rats were sacrificed after 30 days. Some relevant indices were also measured. Results: In the VAD group, the total food intake was significantly lower than that of the control group. After giving vitamin A supplement, the total food intake obviously increased. The expression of MC4R and POMC in the VAD group was increased, while POMC was decreased obviously after vitamin A supplement. Conclusion: Vitamin A affects the expression of related appetite regulator, resulting in the change of food in take.

Abstract:Objective:To explore the expression of colorectal carcinora metastasis associated genel (MACC) in esophageal squamous call careimma(ESCC),and its relatonship with lymphatic metastasis. Methods:Western-blot assays were performed to detect the expression of MACC1 protein in paired sample tissues from 40 ESCC patients and cell lines. Results:The expression levels of MACC1 protein in KYSE510 and EC109 were 0.413 ± 0.175,0.876 ± 0.202,respectively. There were significant differerces in the two ESCC cell lines (P < 0.05). In paired sample tissues from 40 ESCC cases,the expressions of MACC1 protein in tumor tissue specimens and peri-carcinoma tissues were 0.513 ± 0.228 and 0.248 ± 0.179,respectively. There were significant differences in the two tissues,and MACC1 expression in positive lymph mode metastasis speciemens was higher than in other speciemen(P < 0.05).Conclusion: MACC1 may be closely correlated to the oncogenesis,development and metastasis of ESCC.

Abstract:Objective:To explore the application value of glycated hemoglobin (HbA1c) in type 2 diabetes early screening. Methods: A total of 1 089 inhabitants for 40 years old and above were from Huaian area of Jiangsu province. A questionnaire survey was established to collect demographic characteristics, dietary habits, the prevalence of diabetes awareness, and illness history. The measurement of body included: height, weight, waist circumference and blood pressure. Collection of morning urine, fasting venous blood and venous blood was obtained 2 hours after oral glucose powder 82.5 g (except who has a history of diabetes). Detection indexes included routine urine, blood sugar, biochemical full, insulin and glycosylated hemoglobin and glycosylated albumin. Results: FPG diagnosed T2DM sensitivity was 73.89%, while the misdiagnosis rate was 26.11%; HbA1c diagnosed T2DM sensitivity 73.22%, while the misdiagnosis rate was 26.78%; according to FPG joint HbA1c to diagnosis T2DM, the sensitivity was 89.59%, while the misdiagnosis rate was 10.41%. The diagnosis difference of FPG and HbA1c had no statistical significance (P=0.830). The diagnosis alignment was statistically significant, inosculation general (k=0.374, Pk < 0.01). Using HOMA-IR to assess insulin resistance (HOMA-IR), the result of HbA1c≥6.5% subjects were greater than the HOMA - IR diagnostic criteria (HOWA-IR≥2.68) and HbA1c level was positive correlated with HOWA-IR (r = 0.375). Conclusion: FPG joint HbA1c not only greatly improves the diagnostic accuracy of T2DM, also evaluates insulin resistance and the therapeutic effect of diabetes.

Abstract:Objective:To analyze the incidence and risk factors of lower extremity disease in patients with type 2 diabetes mellitus(T2DM). Methods:The experimental group of 95 patients with T2DM and the healthy control group of 53 patients were examined by color Doppler ultrasonography, the incidence rate and characteristics of lower extremity arterial disease of the two groups were compared. According to the presence or absence of lower extremity vascular disease,T2DM were divided into two groups,and then clinical data and biochemical indexes of two groups were analyzed. Results:The rates of intima-media thickness,plaque,stenosis and occlusion of lower limb artery in T2DM were significantly higher than those in the control group,stenosis occurred in the below knee vascular; systolic blood pressure(SBP), body mass index(BMI), two hour postprandial blood glucose(2hPG), glycosylated hemoglobin(HbA1c), high density lipoprotein cholesterol (HDL-C) and total cholesterol (TC) were closely correlated to the pathogenesis of T2DM lower extremity artery. SBP,BMI,2hPG and TC were independent risk factors for T2DM in lower extremity arterial disease. Conclusion:The incidence rate of lower limb arterial disease with T2DM patients is high. Ultrasound can early detect the disease, and actively monitor the related factors of SBP,BMI,2hPG,HbA1c,HDL-C and TC for the control of the occurrence and development of the disease.

Abstract:Objective:To assess the effect of the ratio between contrast medium volume and eGFR for prediction of development of contrast-induced nephropathy (CIN) and the in-hospital prognosis in patients with ST-segment elevation acute myocardial infarction undergoing primary percutaneous coronary intervention (PCI). Method:The study included 215 consecutive patients with STEMI without cardiogenic shock who underwent primary PCI from January 1,2013 to December 4,2013. We calculated their ratio of contrast volume to estimated glomerular filtration rate.ROC methods were used to identify the optimal value of V/eGFR for CIN. The predictive value of V/eGFR was established by Roc curve,and the risk of CIN was assessedusing multivariable logistic regression. Results:Twenty-five(11.6%) patients developed CIN after PCI.V/eGFR is a significantly risk factor of CIN. The ROC curve analysis indicated that a V/eGFR ratio of 2.45 was a fair discriminator for CIN,and a V/eGFR ratio > 2.45 remained significantly associated with CIN (OR 4.15,95%CI 1.19-13.56,P = 0.025). Conclusion:A V/eGFR ratio > 2.45 was a significant predictor of CIN and a poor prognosis in hospital after primary PCI in patients with STEMI.

Abstract:Objective:To analyze risk factors of central venous catheter which is related to infection and to present prevention strategies in critical patients. Methods: This clinical research was conducted on 363 cases of critical patients with central venous catheters via subclavian and jugular veins, to analyze risk factors of central venous catheter related blood stream infection and to present rational prevention strategies. Results: A total of 65 cases of CABI occurred after cental venous catheterization, with a 1 000-day infection rate 18.5‰, and case infection rate of 17.9%. CABI mostly occurred in senile patients (especially over 70 s),who are male, catheter time over 2weeks, or with high APACHE II values, however, nutrition status, MODS and diabetes did not play a critical role on CABI occurrence. Besides, multiple factor analysis reviewing male and catheter time over 2 weeks were closely related with CABI occurrence. Pathogen distribution revealed dominance of gram positive coccus (53.6%), which in proper sequence were staphylococcus epidermidis, aureus, haemolyticus and enterococcus faecium; second predominant pathogens were gram negative bacillus(37.5%), which in proper sequence were acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae; Fungi contributed 7.1% of CABI pathogen, which was mainly candida krusei. Conclusion: In this research, senile critical patients had higher occurrence of CABI, and the main strain of pathogen was gram positive coccus. Risk analysis pointed out that thoese paitents with old age, especially over 70 s, male gender, and with catheter time over 2 weeks and higher APACHE Ⅱ scores suffer high risk of CABI. Meantime, this research did not present remarkable connection between CABI and nutrition status, MODS, and diabetes.

Abstract:Objective:To observe analgesis effects and safety of dezocine at a distinct dose on postoperative pain control in patients undergoing abdominal endoscopic surgery. Methods: Sixty patients (ASA physical status: Grade 1~2) were scheduled to undergo abdominal endoscopic surgeries. Those patients were randomly divided into 3 groups with 20 healthy subjects in each group. The fentanyl group (F group): the patients were given fentanyl (1 μg/kg) intravenously 30 min prior to the end of surgical procedure. D1, D2 group: dezocine (100 μg/kg, 200 μg/kg) was substituted for fentanyl, respectively. The visual analogue scale (VAS) for pain at rest at 2, 4, 24 h and in motion was evaluated postoperatively. The time for wake-up from general anesthesia and sedation status of patients after extubation were also recorded. The incidence of postoperative nausea and vomiting (PONV) in 24 h after surgery was recorded. Results: The VAS values at rest at 2, 4, 24 h time points posterior to extubation in F group were higher as compared with D1, D2 group (P < 0.05). The consumption dose of flurbiprofen for rescue of postoperative pain in F Group was higher than the other two groups (P < 0.05). In addition, the VAS values in motion at 4, 24 h in D2 group were significantly reduced in comparison with D1 group (P < 0.05). The Ramsay score in F group was lower than that in D1 and D2 group (P < 0.05). There was no significant difference in time for wake-up from general anesthesia and incidence of PONV among three groups. Conclusion: Dezocine at 100~200 μg/kg was effective in alleviation of postoperative pain and decreased the postoperative consumption of flurbiprofen as a remedy for pain control. Furthermore, dezocine (200 μg/kg) led to the amelioration of pain in motion and was beneficial in early ambulation for patients.

Abstract:Objective:To construct a recombinant adenovirus that express mouse Ets-1 in primary cultured mouse islets. Methods:To generate pAdTrack-CMV-Ets-1 shuttle vector,the CDS region of Ets-1 was amplified by PCR,and then purified and cloned into the pAdTrack-CMV. pAdTrack-CMV-Ets-1 was recombined with back-bone pAdEasy-1 in BJ5183 bacteria. The resulting vector was transfected into QBI-293A cells to generate recombinant adenovirus. After been amplified and purified,the recombinant adenovirus were used to infect primary cultured mouse islets and INS-1 cells. The protein levels of Ets-1 were determined by Western blotting assay. Results:The Adv-Ets-1 was established successfully and proved to be high infective and possess a high expression potential. Conclusion:The Ets-1 recombinant adenovirus was successfully constructed,which provided a foundation for further study of the function of Ets-1 in primary islets.

Abstract:Objective:To design and compound of twin drug with nNOS-PSD-95 interrupts and gabapentin,pregebalin,and to find new drugs for the treatment of neuropathic pain. Methods:The nNOS-PSD-95 interrupts and gabapentin,pregebalin were connected to form amide. The analgesic effects of target compounds were evaluated by neuropathic pain model. Results:All target compounds showed different degree analgesic effects in neuropathic pain, the analgesic effect of twin drug of nNOS-PSD-95 interrupts and gabapentin,pregabalin twin drug in neuropathic pain was stronger than that of single target drugs. Conclusion:Twin drug with nNOS-PSD-95 interrupts and gabapentin,pregebalin may get a better analgesic effect than that of single target drugs in neuropathic pain.

Abstract:Objective:To study the difference of expression of miRNAs of inflammatory cytokines in lipoplysaccharide(LPS)-stimulated mast cell secretion. Methods:P815 cells were stimulated by LPS (1 μg/ml) for 16 hours. The expression levels of TNF-α and IL-6 in supernatant were detected by ELISA. Real-time PCR was performed to detect the expression differcence of miRNAs after the cells were recollected. Results:In P815 after LPS stimulation,the secretions of TNF-α and IL-6 were largely up-regulated than those of control (P < 0.05). Five miRNAs in the intervention group were found increasingly expressed compared with those in the control group,with miRNA-126 1.3-fold,miRNA-155 1.2-fold,miRNA-223 1.7-fold,miRNA-221 1.2-fold,and miRNA-192 1.4-fold (P < 0.05). Conclusion: miRNAs were expressed differentially in mast cells after LPS stimulation. The differcent expressions of miRNA may be invovled in mast cells function changes.

Abstract:Objective:To observe the correlation of serum follistatin-like 1 (FSTL1) protein level and coronary artery disease in patients with acute coronary syndrome (ACS),and explore the clinical significance of serum FSTL1 level detection in patients with ACS. Methods:Among the total of 123 patients with ACS,25 patients with stable angina and the normal control group of 30 people were recruited. Serum FSTL1 level was measured by enzyme linked immunosorbent assay (ELISA),and the severity of coronary artery stenosis was measured by visual method and quantitative coronary angiography. The SPSS 17.0 software was used for statistical analysis. P value< 0.05 was considered statistically significant. Results:Serum FSTL1 level in all groups with ACS were significantly higher than that in the NC group and the SAP group (P < 0.01). Serum FSTL1 levels in patients with ACS were correlated the scope of coronary artery stenosis (P < 0.01). Serum FSTL1 levels (calculation the natural logarithm of serum FSTL1 levels,LnFSTL1) in the single-vessel disease group (2.38 ± 0.28),the double vessel disease group (2.43 ± 0.26),the multivessel disease group (2.57 ± 0.29) were significantly increased in turn. Moreover,there was statistical difference in each group (F = 5.25,P = 0.007). Conclusion:Serum FSTL1 levels in patients with ACS were significantly increased. The more serious the scope of coronary artery stenosis were,the higher serum FSTL1 levels elevated. Serum FSTL1 levels may have clinical value for the risk stratification and prognosis evaluation of ACS.

Abstract:Objective:To investigate the relationship between the polymorphisms in XPC(rs2228000,rs2228001,rs2470352),XPD(rs13181),XPG(rs17655)and pathologic characterize as well as risk of bladder cancer. Methods:A case-control study was carried out based on 287 patients with bladder cancer and 282 health controls. The genotype of the polymorphisms of XPC,XPD and XPG was detected by massarray SNP. Logistic regression was applied to assess the association between the polymorphisms and risk and pathologic characterize of bladder cancer. Results:There was a significant difference of genotype distribution of XPC rs2228000 in patients and health controls(χ2 =21.949,P < 0.001),and the CT and TT genotype frequencies in patients were higher than those in health controls (CT vs CC:OR=2.01,95% CI 1.41-2.88;TT vs CC:OR = 3.06,95% CI 1.70-5.49);Moreover,the frequencies of those patients who carried T allele were higher than those in controls (CT/TT vs CC:OR = 2.16,95% CI 1.58-3.11). There were significant differences of the genotype frequencies of rs17655,rs2228000 and rs2228001 among the patients with different tumor differentiation degree (rs17655:χ2 = 10.013,P = 0.040;rs2228000:χ2 = 13.836,P = 0.008;rs2228001:χ2 = 14.315,P = 0.006);moreover,a significant difference of genotype distribution of XPC rs2228000 was observed among those patients who with or without tumor distal metastasis (rs2228000:χ2 = 12.204,P = 0.002). Conclusion:The genotype of XPC rs2228000 was associated with the risk of bladder cancer and tumor differentiation degree and distal metastasis,and those who carried with T allele have higher risk of bladder cancer;Moreover,the genotypes of XPC rs2228001 and XPG rs17655 were associated with tumor differentiation degree.

Abstract:Objective:To find out the population’s nutrition healthy status and the prevalence rate of overweight/obesity,hypertension,diabetes and blood lipids abnormalities in the adults in the community of a certain university in Nanjing,then analyze the prevalence and influence of these diseases. Methods:In this study,2 986 serving and retired staffs were selected as sample. Combination of questionnaire investigation, physical examination and biochemical detection were used to collect data. The descriptive analysis was used to analyze the prevalence and the distributive characteristic of risk factors related to chronic disease. The influence factor models of hypertension,hyperlipemia and diabetes were explored. Results:The total incidence of overweight and obesity of staffs was 39.3% and 9.6%,respectively. The rate of overweight in male and female was 45.5% and 29.4%,respectively,and the prevalence rate of obesity was 12.1%and 5.6%,respectively. The rate of overweight and obesity in male was significantly higher than that in female (P < 0.01);The prevalence rate of hypertension was 36.4%. The prevalence rate of diabetes was 5.9%. The total prevalence rate of blood lipid abnormity was 52.2%,there was an increasing tendency in hypertension,blood lipid abnormity with the increase of age and BMI. Conclusion:The problems of overweight/obesity,blood lipid abnormity,hypertension and diabetes in this community are not optimistic. It is urgently needed to improve prevention,detection and treatment for chronic diseases in adults.