Abstract:Objective:To investigate the effect of human umbilical cord mesenchymal stem cells on the proliferation and metastasis of human hepatocellular carcinoma cell line(HepG2)in vitro and in vivo. Methods:The UC－MSCs were cultured 48 hours in serum－free DMEM/F12 medium,and then the supernatant(MSCs－CM)was collected. The MSCs－CM was added to the culture medium of HepG2 cells to a final concentration of 25%,50% or 75%(the experimental groups). DMEM/F12 was set as control group. Cell proliferation was detected by cell counting Kit－8(CCK－8)and the migration and invasion were assessed by Transwell assays. Nude mouse subcutaneous tumor models were established. A total of 12 male nude mice were randomly divided into 3 groups(n = 4,respectively):Group A(subcutaneous injection of HepG2 on the left side),Group B(subcutaneous injection of UC－MSCs + HepG2 on the left side)and Group C(subcutaneous injection with HepG2 on the left side and UC－MSCs+HepG2 on the right side). The volume of tumor was monitored at interval of 7 days until all the mice were sacrificed for the measurements of wet tumor weights at 50th day. Results:After treatment with each concentration of MSCs－CM,the proliferation of HepG2 was significantly promoted(P < 0.05) and the invasiveness was also significantly improved(P < 0.01)compared with the control group,respectively. Significant differences of wet tumor weight and volume were observed between Group A(0.054 2 ± 0.011 2 g)and Group B(0.292 0 ± 0.156 9 g),as well as two sides of Group C(left side:0.089 4 ± 0.024 2 g;right side:0.332 6 ± 0.102 9 g,P < 0.05)mice in a time－dependent manner after 50 days. Conclusion:UC－MSCs can significantly promote proliferation and metastasis capability of HepG2 cells via increasing the release of cytokines of UC－MSCs.

Abstract:Objective:To investigate the expression level of YY1 in gastric cancer,and then detect the effect of YY1 on the proliferation and apoptosis in SGC－7901 cell lines and its possible mechanism. Methods:mRNA and protein expressions of YY1 in cancer and paired adjacent tissues were detected by real－time PCR and immunohistochemistry,respectively. YY1 was up－regulated by a lentiviral vector expression system in gastric cancer cell line SGC－790. CCK－8 was performed to detect cell proliferation,and colony formation assay was performed to evaluate cell growth. Apoptosis was detected by flow cytometry. Results:The expression of YY1 in paired adjacent tissues had a higher level than that in tumor tissues. Compared to the empty vector group (SGC－7901－Empty Vector/SGC－7901－EV) and the normal control group (SGC－7901),the cell proliferation and clonogenicity were significantly decreased as well as cell apoptosis was increased in the over－expressed YY1 group (SGC－7901－YY1). Conclusion:YY1 may participate in the development of gastric cancer. YY1 can play a role as a cancer suppressor gene in the development of gastric cancer,which suggests that YY1 may be a new therapeutic target for gastric cancer.

Abstract:Objective:Metalloproteinases 9(MMP－9)of alveolar macrophages(AM)has been shown to play an important role in the formation of chronic obstructive pulmonary disease(COPD) and emphysema. However,its effect on expression and regulation mechanism is still not clear. This study is aimed to investigate the potential role of sirtuin1(Sirt1)in the expression and regulation mechanism of matrix metalloproteinases 9(MMP－9)in alveolar macrophages(AM). Methods:The AMs were cultured in rats,the simulation model was established using cigarette smoking extract(CSE). To observe change of expression of MM9 in cells. The interference model was established using siRNA to observe expression of MMP－9 after the inhibition of Sirt1. Results:MMP－9 expression increased significantly(P < 0.05)due to 1% CSE stimulation. However,this effect can be antagonized by the Sirt1 agonist resveratrol in a concentration dependent manner. MMP－9 expression in AM cells increased significantly after siRNA interference(P < 0.05). Conclusion:Sirt1 is involved in the expression of MMP9 in AM,and may play a protective role in COPD.

Abstract:Objective:To construct recombinant adenovirus carrying mouse PPA1 gene,and detect its expression in mouse islets and β－TC6 cells,and observe its effect on fatty acid－induced pancreatic β cell apoptosis. Methods:The target gene of PPA1 was cloned into the shuttle vector pAdtracd－CMV. After identified by PCR,the plasmid was linearized by PmeⅠ and recombined with backbone pAdeasy－1 in BJ5183 bacteria. The recombinant plasmid was linearized by PacⅠ enzymes digestion,transfected into QBI－293A cells,and packed for Ad－PPA1 adenovirus. Accoring to the green fluorescent protein (GFP),the titer and infection rate was detected. The mouse islets and β－TC6 cells were infected by recombinant adenovirus and the protein level of PPA1 was detected by Western blot. By Hoechst staining,the effect of PPA1 on cell apoptosis was detected. Results:The recombinant adenovirus vector pAdeasy－PPA1 was successfully constructed. After pAdeasy－PPA1 was transfected into QBI－293A cells,cytopathic effect showed that the adenovirus was successfully packed. The recombinant adenovirus containing mouse PPA1 gene effectively infected mouse islets and β－TC6 cells,and over－express the protein levels of PPA1. Hoechst staining showed that over－expression of PPA1 protected β－TC6 cells against fatty acid－induced apoptosis. Conclusion:The recombinant adenovirus expressing PPA1 gene has been successfully obtained and over－expression of PPA1 has anti－apoptosis effect,which provided a basis for further study of PPA1 on pancreatic β－cell function.

Abstract:Objective:To observe changes of glucose tolerance and insulin function of CETP transgenic mice with ovariectomy and estrogen replacement. Methods:Fifteen female CETP transgenic mice were randomly divided into three groups,including the sham group,the ovariectomy group and the estrogen replacement therapy group. The ovariectomy group and the estrogen replacement therapy group were underwent ovariectomy. Two weeks after operation,the estrogen replacement therapy group was given estrogen,while the sham group and the ovariectomy group were given equal dosages of olive oil. Fasting glucose was measured four weeks after operation. Islets were extracted to test islet function by intraperitoneal glucose tolerance test(IPGTT)and GSIS eight weeks after operation. Results:Mice of the ovariectomy group showed significantly increased fasting glucose levels 8 weeks after operation compared with those of the sham group(P < 0.05). The blood glucose of mice at all time points of IPGTT increased significantly than those in the sham group(P < 0.05). GSIS showed that insulin secretion had a decreased tendency. While after ovariectomy,estrogen replacement therapy significantly improved the above changes. Blood glucose levels at 0,15 and 30 min of IPGTT were decreased significantly(P < 0.05). Insulin secretion function of GSIS also had an improved tendency. Conclusion:After ovariectomy,CETP transgenic mice have impaired islet function and glucose tolerance,which lead to the increase of fasting blood glucose and the decrease of glucose stimulated insulin secretion levels. Estrogen replacement plays a protective role in the harmful effects of ovariectomy on pancreatic is let βcells.

Abstract:Objective:To investigate the regulation of high－mobility group box－1(HMGB1)on P－glycoprotein(P－gp)expression in the hippocampus of epileptic rats. Methods:Sixty male SD rats were randomly divided into the sham operation group,epilepsy group,low,medium and high doses of HMGB1 pretreated groups with 12 rats in each group. Various doses of recombinant HMGB1 protein were injected intraventricularly 15 min before micro－injection of kainic acid(KA)into the hippocampus. The latency period to the onset of stage Ⅲ first seizure time was observed to assess epileptic susceptibility and all the rats were sacrificed after 24 hours of the operation. The damage of the hippocampal tissue,the expression levels of mdr1 mRNA and P－gp were detected by immunohistochemical method,real－time PCR and Western blot,respectively. Results:Compared with the epilepsy group,the SOT of epileptic seizure latency in the medium and high doses of HMGB1 pretreated groups were lower than that of the epilepsy group(P < 0.05),the damage of the hippocampal tissue and neuron loss in the hippocampus CA3 area were increased(P < 0.05),and the expression levels of mdr1 mRNA and P－gp were up－regulated in the medium and high doses of HMGB1 pretreated groups(P < 0.05),but there was no significant differences between the low－doses HMGB1 pretreated group and epilepsy group(P > 0.05). Conclusion:HMGB1 could increase seizure susceptibility,the damage of hippocampal tissue and the over－expression levels of P－gp in the hippocampus of seizure rats.

Abstract:Objective:To construct a hITF gene delivery system,and assess its gene transfection efficiency. Methods:ITF eukaryotic expression vector was constructed. Chitosan nanoparticles were prepared by a complex coacervation method,and its size and morphology were assessed using transmission electron microscope (TEM). Gene transfer capability of nanoparticles was assessed in HEK293 cells. The transcription and expression of hITF were determined by RT－PCR and Western blot. Results:hITF eukaryotic expression vector was successfully constructed. The chitosan nanoparticles were prepared by complex coacervation method with different N/P ratio. Nanoparticle size less than 1 000 nm and narrow distribution of nanoparticle diameter were observed by TEM. The transfection efficiency assessed by fluorescence microscopy and flow cytometry was about 80%,similar to the transfection efficiency of Lipofectamine. RT－PCR and Western blot assay demonstrated that hITF was transfected and expressed successfully. Conclusion:An optimal hITF gene delivery system was successfully constructed. This research laid foundations for further application of hITF gene therapy.

Abstract:Objective:To observe the paracrine effects of mesenchymal stem cells(MSC)on the activated hepatic stellate cells Lx－2,and explore the possible mechanisms. Methods:A co－culture system was established by culturing bone marrow mesenchymal stem cell(BM－MSC)in the Transwell insert and Lx－2 on the plastic plates(6 well),which were placed on the upper and lower layer repectively,and set it as the experiment group. Normal Lx－2 was cultured alone as control. Cell apoptosis was determined by Hoechst 33342 staining and flow cytometry,respectively. The expressions of uncoupling protein 2(UCP2)mRNA and protein in Lx－2 were detected by quantitative real－time PCR(qRT－PCR)and Western blot,respectively. Intracellular and mitochondrial reactive oxygen species(ROS)levels of Lx－2 were measured by fluorescent probe method. The content of MDA in co－culture supernatant was determined by thiobarbituric acid(TBA)reaction. Results:Compared with the control group,Hoechst 33342 staining showed nuclear condensation,granular fluorescence and other typical features of apoptosis in parts of Lx－2 cells. The apoptotic rate of Lx－2 in the experiment group was 2.6 times of that in the control group(P < 0.05). The expressions of UCP2 mRNA and protein in Lx－2 were significantly inhibited compared with those in the control group(P < 0.05). The intracellular and mitochondrial ROS levels of Lx－2 in the experiment group were highly enhanced. The concentration of MDA in the co－culture supernatant in the experimental group was (0.43 ± 0.47)mmol/L,which was significantly higher than the control group with (0.16 ± 0.43)mmol/L(P < 0.05). Conclusion:The paracrine effects of MSC were capable of inducing Lx－2 apoptosis,which is supposed to be the result of the suppression of UCP2 and over－expression of intracellular and mitochondrial ROS in Lx－2.

Abstract:Objective:To evaluate the degree of pressure overload and successful ligation of transverse aortic constriction (TAC) using ultrasonic Doppler methods and investigate whether different gradient TAC made any differences in cardiac structure and function. Methods:A total of 60 male C57/BL6 mice were randomly divided into the sham－operated group (sham,n = 20)the 26 G needle banded TAC group (TAC26G,n = 20) and the 27 G needle banded TAC group (TAC27G,n = 20). The degree of pressure overload and successful ligation of TAC were determined by measuring the flow ratio defined by the right carotid arteries (RCA)/left carotid arteries (LCA). The effect of pressure overload on cardiac structure and function due to different gradient TAC were measured by calculating the heart weight/body weight (HW/BW) and left vetricle weight/tibia length(LVW/TL) and echocardiography. Results:Doppler assays showed that the RCA/LCA was significantly increased in both TAC26G and TAC27G compared with sham (P < 0.05). The ratio of RCA/LCA in TAC27G was significantly different from TAC26G (P < 0.05). Two weeks after the operation,compared with sham,IVSd and LVPWd were significantly increased in both the TAC26G and TAC27G group (P < 0.05);EF and FS were significantly increased in the TAC26G group but significantly decreased in the TAC27G group (P < 0.05). Two weeks after the surgery,the HW/BW and LVW/TL were significantly increased in both TAC26G and TAC27G compared with sham (P < 0.05),and the above index were much higher in the TAC27G group than the TAC26G group (P < 0.05). Conclusion:Our data revealed the relationship between carotid velocity change and cardiac hypertrophy in early the phase of transverse aortic constriction. It provided guidance on a better mouse model for pressure overload induced cardiac hypertrophy and heart failure.

Abstract:Objective:To establish a neonatal rat model of acute lung injury(ALI)induced by intraperitoneal administration of various doses of lipopolysaccharide(LPS)and investigate the role of receptor for advanced glycation end－products(RAGE)in ALI. Methods:Thirty newborn rats were randomly divided into five groups(six in each group)according to the different doses of LPS(0.3,1,3 and 9 mg/kg)and saline. All the rats were sacrificed and observed 24 h later. Levels of tumor necrosis factor alpha(TNF－α)and soluble RAGE(sRAGE)in the plasma and bronchoalveolar lavage fluid(BALF)were detected by enzyme－linked immunosorbant assay(ELISA). RAGE mRNA levels in tissue homogenates were detected by RT－PCR and RAGE protein levels by Western blot. The pathological assessment of lung tissues was performed by HE staining. Results:The LPS 9mg/kg group was excluded due to its high mortality(83.3%). When compared to the control group,the levels of TNF－α,the expression of RAGE protein and RAGE mRNA,and the lung damage scores in the other four groups were increased. All these parameters increased in a dose－dependent manner in the LPS groups,while the levels of sRAGE in BALF decreased. Conclusion:LPS intraperitoneal injection can induce ALI in neonatal rats. LPS dosage of 3 mg/kg may be the optimal dose for ALI model in neonatal rats. RAGE can be used as a sensitive indicator of ALI.

Abstract:Objective:To establish an animal model of hind limb ischemic preconditioning (RIPC) and to study the protective effect of remote ischemic preconditioning on renal ischemic reperfusion injury in rats. Methods:A total of 48 healthy Sprague Dawley rats were subjected to right nephrectomy,and then randomly divided into four groups:the sham operation (Sham),the simple hind limb ischemic preconditioning group (LIP),the simple renal ischemia reperfusion group (IR),and the hind limb ischemic preconditioning－renal ischemia reperfusion group (LIP－IR). After 24 h of reperfusion,serum creatinine (Scr) and blood urea nitrogen (BUN) were tested,pathological changes of renal tissues were observed,renal tissues were collected for the determination of NO and TNOS,iNOS and cNOS activity. Results:The level of Scr and BUN and the renal tubule injury score,and the activity of TNOS and iNOS in the kidney tissues were significantly decreased in the LIP－IR group as compared with those in the IR group (P < 0.05),but the level of NO and the activity of cNOS in the kidney tissues were significantly increased (P < 0.05). Compared with the sham group,the above indicators in the LIP group have no significant difference (P > 0.05). Conclusion:Limb ischemic preconditioning can protect kidney from ischemic reperfusion injury. The protection may be related to the increased expression of eNOS as well as the reduced expression of iNOS in the kidney.

Abstract:Objective:Studies showed that Newcastle disease virus (NDV) had significant inhibitory action on many tumors in vitro and in vivo. This experiment was designed to investigate the effects and possible mechanism of NDV－D90 strain on breast cancer MCF－7 cells in vitro. Methods:Growth inhibition of MCF－7 cells was detected by CCK－8 method. The morphological changes of MCF－7 cells caused by D90 strain were observed by optical microscope and DAPI staining. MCF－7 cell apoptosis rate was detected by flow cytometry. The expression of apoptosis related proteins of MCF－7 cells was detected by Western blot. Results:NDV－D90 strains had inhibitory effect on MCF－7 cells in a concentration and time dependent manner;D90 strain induced apoptosis of MCF－7 cells and regulated the expression of apoptosis related proteins,including up－regulating Bax expression,down－regulating Bcl－2 protein,and promote the cleavage and activation of Caspase－3 and Caspase－9. Conclusion:D90 strain could regulate the expression of apoptosis related protein,promote the cleavage and activation of Caspase－3 and Caspase－9. It could induce apoptosis of MCF－7 cells through the mitochondrial pathway in a concentration and time dependent manner.

Abstract:Objective:To explore the protective effect of taurine on injured neurons induced by hydrogen peroxide (H2O2). Methods:Neurons were treated with different doses of H2O2. Oxidative stress model was prepared in vitro. Taurine was applied at 5,15 or 25 mmol/L 24 h prior to H2O2 treatment. Specific fluorescent probe DCFH－DA was performed to analyze reactive oxygen species level of neurons. Lactic dehydrogenase (LDH) release assay was performed to detect the injury degree of neurons. Brain－derived neurotrophic factor (BDNF) and synaptic－related protein levels were evaluated by Western blotting. Results:Compared with the control group,different doses of H2O2 (5,50 and 100 μmol/L) increased LDH release to the extracellular medium (P < 0.05),while 15 and 25 mmol/L taurine decreased the release (P < 0.05). One hundred μmol/L H2O2 led to an increase of reactive oxygen species level and decreased BDNF,synapsin and spinophilin protein expressions (P < 0.05). After treatment with 25 mmol/L taurine,the above indicators were attenuated (P < 0.05). Conclusion:Taurine can alleviate H2O2 induced neuron damages and show neuroprotective effect against oxidative stress.

Abstract:Objective:To evaluate the correlation between serum leptin with clinical and blood biochemical indexes,and longitudinal changes of above parameters after parathyroidectomy (PTX) in stage 5 chronic kidney disease(CKD) patients. Methods:This cross－sectional study not only included 113 stage 5 CKD patients together with 76 healthy controls but also contained a follow－up of two subgroups classified as successful (n = 23) and unsuccessful (n = 4) PTX. Clinical characteristics,blood biochemistry and serum leptin levels were measured. Results:Serum leptin concentration was positively correlated with age and body mass index (BMI),but negatively correlated with serum albumin (Alb) level in healthy men. In healthy women,serum leptin concentration was positively correlated with BMI. There was no significant difference of serum leptin between healthy controls and stage 5 CKD patients. Serum leptin concentration in patients with stage 5 CKD was correlated positively with BMI,serum glucose,serum triglyceride (TG) and serum Alb in males and BMI,hemoglobin (Hb),hematocrit (Hct) and serum TG in females. However,serum leptin had a negative relationship with serum high density lipoprotein (HDL) and serum intact parathyroid hormone (iPTH) in males and only serum HDL in females. Compared with baseline,the successful PTX subgroup had significant improvement in serum leptin level. Increased serum leptin change was positively related to increased weight,Hb and Hct change as well as decreased lgiPTH change in this group. There was no improvement in the above parameters in the unsuccessful PTX group. Conclusion:Serum leptin level has no significant difference between stage 5 CKD patients and health subjects. However,it is associated with bone－mineral metabolism,hematopoiesis and nutritional indexes in stage 5 CKD patients. Successful PTX contributes to increased serum leptin level and improved nutrition,anemia and bone－mineral metabolism..

Abstract:Objective:To analyze the possible association between VDAC2 gene polymorphism and sperm quality of idiopathic male infertility. Methods:A genetic typing of VDAC2 gene locuses (rs2804535,rs7896741,rs11001334 and rs1259503) by TaqMan SNP genotyping technique and a semen analysis by computer－assisted semen analysis (CASA) were respectively performed in 523 Han－Chinese males with idiopathic infertility in order to analyze the correlation between gene polymorphism and sperm quality. Results:No significant association between genotypes and semen quality was found at locuses rs2804535 and rs7896741. However,at rs 11001334,it was found that the TT genotype showed a significant lower sperm concentration compared with the CC genotype (P = 0.045). Moreover,compare with CC genotype,the sperm motility of CT genotype had a significant increase (P = 0.026);meanwhile,the same trend was found in the combination of CT and TT genotypes (P = 0.037). However,at rs1259503,the combination of GC and CC genotypes had a remarkable reduction of semen volume than GG genotype (P = 0.039). Conclusion:These findings indicate that the gene polymorphism in the VDAC2 gene may be associated with semen quality.

Abstract:Objective:To explore the relationship between C－reactive protein (CRP) elevation,overweight,obesity as well as their interactions and diabetes risk. Methods:Stratified cluster sampling method was and 2 400 residents aged over 40 years were selected. A pre－tested questionnaire was performed to collect the demographic information,lifestyle,history of disease,family history of diabetes and etc. Anthropometric measurements including height,weight,wait circumference (WC) and blood pressure (BP) were performed at the time of interview. Fasting blood sample was also collected for the detection of CRP,fasting blood glucose (FBG) and etc. Logistic regression method was applied to evaluate the relationship between CRP elevation (> 3 mg/L),overweight,obesity and diabetes risk. Odds ratio (OR) and 95% confidence interval (CI) were calculated. Interactions were estimated on both additive scale and multiplicative scale. Results:A total of 1 899 subjects were interviewed and recruited for the analysis,the responding rate was 79.13%. Among them,238 type 2 diabetes cases were identified. Comparing with the normal level (CRP≤3 mg/L),the adjusted OR for CRP elevation with diabetes (CRP> 3 mg/L) was 1.74 (95%CI:1.24~2.45);the OR for high WC,BMI getting overweight and obesity was 1.41 (95%CI:1.06~1.89),1.70 (95%CI:1.12~2.44) and 2.22 (95%CI:1.39~3.54),respectively. Individuals with elevated CRP level,overweight or obesity were at the highest risk of diabetes,whereas no significant interaction was observed. Conclusion:High CRP level,overweight and obesity are associated with increased risk of type 2 diabetes. Reducing CRP,BMI and WC level through interventions may postpone or reduce the development of type 2 diabetes.

Abstract:Objective:To investigate factors associated with late－onset fetal growth restriction (FGR) and their impacts on perinatal outcomes. Methods:Maternal and neonatal data from 140 cases of late－onset FGR were collected from Nanjing Maternity and Child Health Care Hospital affiliated to Nanjing Medical University from May 2012 to October 2013. All cases were divided into several groups according to pregnancy complications,gestational weeks (32 to 33+6 weeks for group Ⅰ,34 to 36+6 weeks for group II,37 to 40+4 weeks for group Ⅲ) and delivery modes. Neonatal physical development and morbidities of common diseases were compared among groups. Results:The birth weight and body length of newborns of the group with pregnancy complications were significantly lower than those without pregnancy complications. The morbidities of neonatal septicemia,neonatal pneumonia and acute respiratory distress syndrome (ARDS) of the group with pregnancy complications were significantly higher than those without pregnancy complications. The regression coefficient between neonatal physical development indexes and total gestational weeks was all less than group Ⅱ,and there were no regression relation between neonatal physical development indexes and groupⅠand Ⅲ. The morbidities of neonatal septicemia,neonatal pneumonia and ARDS of groupⅠandⅡwere significantly higher than those of group Ⅲ. The birth weight and body length of term newborns of the cesarean group were significantly lower than those of the vaginal delivery group. No other significant differences were observed between the two groups. Conclusion:The late－onset FGR fetuses display rapid growth and development in pregnancy 34 to 36+6 weeks and the common diseases morbidities of term newborns are relatively low. Therefore,gestational weeks could be extended to 37 weeks if only intensive therapy and close monitoring are available. The perinatal outcomes of late－onset FGR were relatively good;therefore,vaginal delivery with intensive care could be recommended.

Abstract:Objective:To explore reliable and efficient sampling methods and corresponding formulae for quantitative sensitive question survey on three－stage random sampling. To provide scientific data for the prevention and control of high risk AIDS population. Methods:We derived statistical formulae from mathematical statistics theory;investigated MSMs in Beijing for instance;used SAS programming to simulate investigation and analysis of 100 samples through Monte Carlo simulation and accessed validity and reliability of methods and formulae studied in this article. Results:We derived statistical formulae of the overall mean estimator and its variance on quantitative sensitive questions under stratified three－stage sampling. The means of ages of the first occurrence of MSM,the monthly number of different sexual partners and the monthly number of sex were 21.96 years old,2.80 people,4.85 times,respectively,among MSM in Beijing,and their standard errors were 0.127 years old,0.096 people,0.559 times,respectively. All of the population mean of 95% confidence interval obtained from 100 analog samples contains population mean. Conclusion:For high－risk sexual behavior among MSM with AIDS,its prevention and control should be strengthened. Monte Carlo computer simulation results show that stratified three－stage sampling method and corresponding formule are feasiblae and could be applied in the sensitive issue of large－scale sampling in the future.

Abstract:Objective:To investigate the effect of the syphilis prevention project of mother－to－child transmission in rural area of Jiangsu Province and make the rational planning for improving the project. Methods:A questionnaire survey was carried to interview 459 cases of maternal syphilis in rural area of Jiangsu Province who were diagnosed since the project started in 2010 to understand the control effect. Results:Ninety point two percent of 459 cases of maternal syphilis were administered with drug treatment,and the rate of standard treatment was 85.6%. Ninety－four percent of maternal syphilis patients had live births and no cases of congenital syphilis were found,and there were regional differences among southern Jiangsu,middle Jiangsu and northern Jiangsu (P < 0.05). Twenty－five point seven percent of staffs met the phenomenon of non－compliance with regional differences among southern Jiangsu,middle Jiangsu and northern Jiangsu (P < 0.001). The main factor influencing non－compliance was that pregnant patients with syphilis were worried about the confidentiality of their information. Conclusion:The project promotes the prevention of mother－to－child transmission of syphilis,but there are lots of non－compliance and non－standard treatment,which are different among different regions.

Abstract:Objective:To investigate the possibility of quantitive analysis of 99mTc－HYNIC－PEG4－E[PEG4－c(RGDfk)]2 (99mTc－3P4－RGD2) uptake and tumor integrin αvβ3 expression in glioma bearing nude mice with microSPECT/CT imaging. Methods:microSPECT/CT imaging of 99mTc samples with different volume and radioactivity was carried out to assess the influence on radiocounting based upon volume and activity. 99mTc－3P4－RGD2 MicroSPECT/CT imaging was also carried out to glioma bearing nude mice. Tumor volume was determined in vivo by caliper,microCT,microSPECT and microSPECT/CT. The consistency between determined volume and actual volume was analyzed by Bland－Altman plots. Tumor uptake of 99mTc－3P4－RGD2 was quantified to estimate glioma expression level of integrin αvβ3 and confirmed by immunohistochemistry examination. Results:Linear relationship with R2 being 0.999 7 was found between the radioactivity counts from the SPECT/CT fusion images and those from γ－counter. Bias of microSPECT/CT measurements (%) compared with the reference ones was (-2.48 ± 8.17),less than that of microCT (15.04 ± 79.53,t = 5.60,P = 0.005),microSPECT (17.04 ± 36.27,t = 7.17,P = 0.002),respectively. MicroSPECT/CT can reflect vial and necrotic tumor volume and tumor uptake of 99mTc－3P－RGD2 was positively correlated with integrin αvβ3 expression with R2 being 0.95. Conclusion:MicroSPECT/CT imaging,which is more accurate in evaluating via tumor volume and integrin αvβ3 expression level,is of potential value for patients screening for integrin αvβ3 receptor targeted therapy and dose individualization.

Abstract:Objective:By comparing with conventional coronary angiography(CAG),we sought to investigate the diagnostic value of dual－source CT coronary angiography(DSCTA)to diabetic patients with coronary artery stenosis. Methods:A DSCTA inspection was established to 81 cases of type 2 diabetes in patients with suspected coronary heart disease,along with a variety of scanned images reconstructed by the Workstation. We then assessed 12 segments of the proximal diameter ≥2.0 mm,selected patients of stenosis degree ≥50%,and adopted 2x2 contingency table with chi－square test. Results:A total of 68 cases showed stenosis ≥50% in all 81 patients,and 42 out of these 68 patients(61.8%)had multiple－vessel stenosis,and CAG examination was performed in all these 68 patients. Sixty－eight patients along with a total of 793 coronary artery segments were clear and evaluable,with the evaluable rate of 97.2%. CAG was set as the gold standard,and the predicted values of sensitivity,specificity,positive predictive value,negative with DSCTA detected vascular stenosis ≥50% were 95.6%,97.7%,89.7% and 99.1%,respectively. The difference between the two methods was not statistically significant(P > 0.05). The sensitivity(97.4% vs 93.2%),specificity(97.6% vs 97.9%),positive predictive value(89.4% vs 90.2%),and negative predictive value(99.5% vs 98.6%) of DSCTA detected coronary artery stenosis between rate < 70 beats/min and ≥70 beats/min in diabetic patients was not significantly different(P > 0.05). Conclusion:DSCTA has clear image,rapid scanning speed and is non－invasive,etc,which is not influenced by the heart rate. It is an effective coronary artery screening method,especially for type 2 diabetic patients with coronary heart disease.

Abstract:Objective:To investigate the effect of P38 phosphorylation mediated by microtubules polymerizationon and the mechanism of As2O3 induced apoptosis in cisplatin resistant gastric cancer cells. Methods:Cisplatin resistant cells SGC7901/DDP were produced from parental SGC7901 cells by persistent gradient exposure to cisplatin. CCK8 and flow cytometry assays were performed to detect the cytotoxicity of As2O3. The change in the polymerization and drug－fast of tubulin was observed by immunofluorescence microscopy. Results:CCK8 and flowcytometry assay indicated that,when compared with SGC7901,cisplatin resistant SGC7901/DDP cells enhanced anti－apoptosis capacity to As2O3. The expression level of p－P38 was significantly restrained in SGC7901/DDP cells with As2O3 treatment,whereas it was closely related to stability of microtubules in SGC7901/DDP cells. The inhibition of phosphorylation of P38 in SGC7901 cells significantly reduced tubulin polymerization and apoptosis produced by As2O3 treatment. Conclusion:The development of multidrug resistance in cisplatin resistant SGC7901/DDP cells gastric cancer cells to As2O3 was associated with the phosphorylation of P38 mediated tubulin polymerization,and tubulin polymerization plays an important role in the tolerance of As2O3 by gastric cancer cell.