• Volume 0,Issue 9,2014 Table of Contents
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    • The role of renal microvascular endothelial-mesenchymal transition in transplant renal interstitial fibrosis formation

      2014(9):1149-1156. DOI: 10.7655/NYDXBNS20140901

      Abstract (5829) HTML (77) PDF 17.46 M (11493) Comment (0) Favorites

      Abstract:Objective: To explore the role and mechanism of human renal microvascular endothelial-mesenchymal transition (EndMT) in transplant renal interstitial fibrosis formation. Methods:Based on serum and renal allograft tissue samples from 25 cases of chronic renal allograft dysfunction (CAD) patients and 25 normal renal tissue samples and serum, we observed the changes of renal function and the degree of renal tubular atrophy,renal glomerular collapse and interstitial fibrosis by blood biochemical,periodic acid-schiff staining (PAS) and Mason trichromatic staining assays. Using immunohistochemical and indirect immunofluorescence double staining assays,we also detected the expression and distributions of vascular endothelial cell marker CD34 and myofibroblast marker α-Smooth muscle actin (α-SMA),and transforming growth factor-β1 (TGF-β1) in renal tissue samples of the two groups. Human umbilical vein endothelial cells (HUVECs) set as the object of study and stimulated by TGF-β1 (5 ng/ml) respectively for 0~72 h in vitro. Western blotting was used to observe the expression of CD34 and α-SMA. Results:Compared with the normal group,serum creatinine levels in the CAD group increased significantly. The results of PAS and Masson staining showed that renal tubular atrophy,glomerular collapse and interstitial fibrosis were more apparent in transplanted kidneys of the CAD group. Indirect immunofluorescence staining and immunohistochemical results showed that the positive stains of CD34 reduced,while the positive expression of α-SMA and TGF-β1 increased significantly in the CAD group compared with the normal group. Indirect immunofluorescence double staining showed that the double positive stains of CD34 and α-SMA could be found in the parts of glomerular and interstitial microvascular endothelial cells in the CAD group. The results of Western blotting demonstrated that after treatment with TGF-β1,the expression of CD34 reduced and α-SMA expression increased in an time-dependent mean in HUVECs as compared with the normal group (P < 0.01). Conclusion:Human renal microvascular EndMT may be mediated by TGF-β1 and plays an important role in transplant renal interstitial fibrosis formation.

    • AngiotensinⅡ preconditioning prevents mesenchymal stem cells from apoptosis

      2014(9):1157-1162. DOI: 10.7655/NYDXBNS20140902

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      Abstract:Objective:To examine whether AngⅡ preconditioning improves the anti-apoptotic ability of bone marrow mesenchymal stem cells (MSCs),and if so,what is its possible mechanism. Methods:MSCs cells from rats were pretreated with Ang Ⅱ , and apoptosis of MSCs was induced by H2O2 or hypoxia combined serum deprivation (Hypoxia/SD) method. Cell viability and apoptotic proportion were detected by lactate dehyarogenase (LDH) release, methye thiazolye telrazlium (MTT) assays and Hoechst 33342 staining. Annexin V-FITC assay was performed for quantification of apoptosis rate. The mitochondrial transmembrane potentia (ΔΨm) was detected by a flow cytometer using ΔΨm specific stain Rhodamine 123. Results:AngⅡ preconditioning significantly reduced H2O2- or Hypoxia/SD-induced cell damage,increased cell viability,reversed Hypoxia/SD-induced mitochondrial membrane potential reduction. Both of ERK1/2 inhibitor U0126 and Akt inhibitor LY294002 significantly attenuated the the protective effects of AngⅡ preconditioning on the viability and apoptosis rate of MSCs. Conclusion: AngⅡ preconditioning prevents MSCs from apoptosis,and Akt and ERK1/2 signalling pathway may be implicated in the effect of AngⅡ.

    • Calcium sensing receptor improves long bone formation and cartilage mineralization of mice

      2014(9):1163-1167. DOI: 10.7655/NYDXBNS20140903

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      Abstract:Objective:To determine the direct action of calcium sensing receptor (CaSR) on both long bone and cartilage mineralization of mice. Methods:The femur and tibia of both 3-day-old wild-type and CaSR gene knockout mice from the same brood were transplanted into the latissimus dorsi muscle of 8-week-old wild type mice with the same parents of the 3-day-old mice. After 4 weeks,the femur and tibia were analyzed with X-ray and histology methods. Results:CaSR knock-out mice exhibited growth retardation and the hypertrophic zone of cartilage was obviously wider than that of the wild-type mice (P < 0.001). Conclusion:CaSR have a direct promoting action on long bone growth,and can stimulate the mineralization and ossification of hypertrophic chondrocytes.

    • Protective effects of asiatic acid against postoperative cardiac hypertrophy induced by transverse aortic constriction in mice

      2014(9):1168-1172. DOI: 10.7655/NYDXBNS20140904

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      Abstract:Objective:To determine the protective effects and molecular mechanisms of asiatic acid (AA) on postoperative cardiac hypertrophy induced by transverse aortic constriction in mice. Methods:Transverse aortic constriction (TAC) was performed to establish the mice model of myocardial hypertrophy. C57BL/6 mice were divided into five groups:the Sham group,the TAC group,the TAC+AA1[25 mg/(kg-day)] group;the TAC+AA2 [50 mg/(kg-day)] group and the TAC+AA3 [100 mg/(kg-day)] group. After 2 weeks of TAC,echocardiography was performed to measure the hypertrophic criteria of the hearts. The protein expressions of TGF-β1,p-p38,p38,p-ERK1/2 and ERK1/2 were detected by Western blot. The mRNA expressions of ANP and TGF-β1 were detected by real-time PCR. Results:AA significantly inhibited cardiac hypertrophy induced by TAC,improved cardiac function,reduced the mRNA and protein expression of TGF-β1,as well as the phosphorylation of p38 and ERK1/2. Conclusion:AA attenuates TAC-induced cardiac hypertrophy. The protective mechanisms of AA may through inhibiting TGF-β1-p38/ERK1/2 signaling pathway.

    • Comparison of different treatment methods for electrical storms during establishment swine model of acute myocardial infarction

      2014(9):1173-1178. DOI: 10.7655/NYDXBNS20140905

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      Abstract:Objective:The aim of this study was to compare different treatment methods for ventricular eleatrical storm(VES) during establishment of swine model of acute myocardial infarction (AMI). Methods:Thirty-two swine were divided into four groups. Group A,sham group with 7 swine,saline was given by ear vein;group B,8 swine,amiodarone was given;group C,8 swine,metoprolol was given;group D,9 swine,amiodarone and metoprolol were given simultaneously. After anesthesia,each of those groups were implanted with balloons in the mid-distal left anterior descending artery,by inflating or defating the balloons,ischemic preconditioning was performed 3 or 4 times,then sustained occlusion of LAD for 60 min,at last reperfusion performed for 30 min. Results:There were 3 unexpected deaths in the 4 groups. Various arrhythmias occoured during ischemic preconditioning,sustained occlusion and reperfusion period. Compared with group A,there was a decreasing trend of various arrhythmias in groups B,C and D,but the difference was not significant(P > 0.05). Compared with group A,there was a decreasing trend of VES in groups B and C,but the difference was not significant(P > 0.05),but group D deceased significantly(P = 0.005). Correspondingly,compared with group A,there was a decreasing trend of death due to VES in groups B,C and D,but the difference was not significant(P > 0.05). There were no significant difference of venricular arrhythmias uring ischemic preconditioning,sustained occlusion and reperfusion. Deaths duo to VES were increased during ischemic preconditioning and reperfusion compared with sustained occlusion,but the difference was not significant(P > 0.05). Conclusion:VES and deaths duo to VES are more likely to occur during AMI. Combination of amiodarone and metoprolol can effectively reduce the occurrence of VES are deaths due to VES in the swine model of AMI.

    • Effects of carnosine on IFN-γ and IL-4 in peripheral blood of rats with focal cerebral ischemia

      2014(9):1179-1183. DOI: 10.7655/NYDXBNS20140906

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      Abstract:Objective:To explore the effect of carnosine on peripheral blood IFN-γ and IL-4 in rats with focal cerebral ischemia. Methods:Thirty male SD rats were randomly divided into 3 groups:the sham-operated group (n=10),the model group (n=10) and the carnosine-treated group(n=10). The rat model of the right middle cerebral artery occlusion (MCAO) was established by suture method in model group and the carnosine-treated group. After making the MCAO model,the rats in the carnosine-treated group were lavaged with soluble carnosine once a day at the doses of 1 g/(kg-d) as the criteria of their body mass,and the same volume of normal saline was lavaged in the sham operation and the model group. The neurological function was scored with Longa 5-point scale at postoperative 24 h and 72 h. The levels of IFN-γ and IL-4 in peripheral blood were detected by ELISA method at 24 h and 72 h. Brain tissues were obtained for HE staining at postoperative 72 h and observed by pathological histology. Results:Compared with the model group,there were significant differences in the neurological outcome scores in the carnosine-treated group at 24 h and 72 h (P < 0.05),There was a significant decrease at 24 h and 72 h of the carnosine-treated group (P < 0.05). At 24 h and 72 h,compared with the sham-operated group,the level of IFN-γ and IFN-γ/IL-4 ratio were significant decreased (P < 0.05). The level of IL-4 was significant increased (P < 0.05). After cerebral ischemia,neurons in cerebral cortex of rats were severe necrosis and the pathological injury of cerebral cortex in rats was obvious. After carnosine treatment,secondary neuron injury mitigated. Conclusion:Peripheral immune suppression exists in rats with focal cerebral ischemia. Carnosine could has potential protective effective on peripheral immune suppression of MCAO rats and the mechanism could be IFN-γ,IL-4 and IFN-γ/IL-4 ratio elevation.

    • L.paracasei inhibits LPS-induced mononuclear phagocytes inflammatory cytokine response through negative regulators

      2014(9):1184-1191. DOI: 10.7655/NYDXBNS20140907

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      Abstract:Objective:To investigate the regulation mechanism of prestimulation of Lactobacillus paracacei (L.para) inhibited on LPS-induced inflammatory cytokine release. Methods:THP-1 cells were differentiated to macrophage-like cells by PMA stimulation. Cells were then pretreated by L.para (the experimental group),a lower dose LPS (10 ng/ml) and TLR2 agonist Pam3CSK4 respectively (the control group). After 24 hours pretreatment,THP-1 cells were further stimulated with a higher dose LPS (1 -滋g/ml). The influence of this prestimulation on the expression of TLR signaling pathway negative regulators,membrane expression and mRNA levels in TLR4 and CD14 and IL-1β and TNF-α levels in a higher dose LPS-induced inflammatory cytokines were respectively detected. mRNA levels were measured by real-time quantitative polymerase chain reaction (RT-qPCR). Membrane expressions of TLR4 and CD14 after pretreatment were confirmed by flow cytometry. Results:LPS-induced IL-1β and TNF-α levels were significantly reduced by L.para pretreatment;In addition,the mRNA level of TLR signaling pathway negative regulators A20,SOCS1,SOCS3 and IRAK3 were upregulated while membrane expression of TLR4 and CD14 unaltered. Prestimulation-reduced negative regulators expression levels along with the release of LPS-reduced inflammatory cytokines were weaken by a IRAK1/4 inhibitor. Conclusion:L.para prestimulation could enhance negative regulators of TLR signaling A20,SOC1,SOCS3 and IRAK3 expression thus inhibits inflammatory response of macrophage after LPS stimulation.

    • Significance of TWIST gene and protein in the occurrence and development of cervical cancer

      2014(9):1192-1195. DOI: 10.7655/NYDXBNS20140908

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      Abstract:Objective:To detect the expression of TWIST gene in cervical cancer and normal cervical tissues,explore the correlation between TWIST gene and the development of cervical cancer,and assess the clinical application of TWIST gene detection for cervical cancer diagnosis. Methods:The primary tumor tissues from 56 cervical cancer patients and 35 normal cervical tissues were collected. The expression of TWIST mRNA was detected by Real-time PCR. TWIST protein levels were determined by Western blot. The correlation analysis was conducted to assess the relationship between TWIST expression and clinical pathological parameters,load of high risk human papilloma virus (HPV). Results:The results of Real-time PCR showed that the expression rate of TWIST gene in the cervical cancer tissues was significantly higher than that in normal cervical tissues (P< 0.01),which was verified by the expression of TWIST protein levels detected by Western blot(P < 0.01). TWIST expression in the cervical cancer tissues was correlated with lymph node metastasis(P = 0.02) ,but not with age,FIGO stage,tumor size,pathological type and the differentiation grade of tumor (both P > 0.05). Logistic regression analysis showed that high level of TWIST mRNA expression was an independent risk factor for lymph node metastasis (P < 0.05). There was no correlation between TWIST expression and load of high risk HPV in the cervical cancer group(r = 0.205,P > 0.05). Conclusion:TWIST expression in the cervical cancer tissues was up-regulated,and associated with lymph node metastasis. It may play an important role in the pathogenesis and metastasis of cervical cancer.

    • Effects of quorum sensing molecule on the morphology in Candida albicans biofilm

      2014(9):1196-1201. DOI: 10.7655/NYDXBNS20140909

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      Abstract:Objective:Quorum sensing regulates the formation of Candida albicans (C. albicans) biofilm. Farnesol and tyrosol are quorum sensing molecules produced by C. albicans. The aim of this study is to find the effects of quorum sensing molecules on the formation of C. albicans and biofilm in different time phases of biofilm. Methods:C. albicans biofilm was constructed in vitro. The study C. albicans biofilm were separated into the tyrosol treated group,the arnesol treated group,the tyrosol and farnesol co-treated group and the no-treated control group. The effects of the tyrosol and farnesol on C. albicans cell viability in different time phases were detected by XTT method. The effects of the tyrosol and farnesol on C. albicans biofilm formation were observed by scan electronic microscope (SEM). The production of farnesol and tyrosol of biofilms was detected by gas chromatography-mass spectrometry (GC/MS) and high press liquid chromograph (HPLC),respectively. Results:The XTT method suggested that biolfilm activitity was promoted by tyrosol at 6 h (P < 0.05),while it was suppressed by farnesol at 3 h and 6 h (P < 0.05). Biofilm activity of the tyrosol and farnesol co-treated group was inhibited at 6 h (P < 0.001). The SEM observations showed that tyrosol contributed to the germ tube formation of the C. albicans biofilm at 3 h and 6 h. Farnesol inhibited the hyphae growth of biofilm at 3 h and 6 h. The tyrosol and farnesol co-treated group showed that the effect of tyrosol was not antagonistic to that of farnesol. GC/MS and HPLC results showed that C. albicans produced little tyrosol and farnesol at early stage and showed no obvious rising trend (P > 0.05). With biofilm maturation (24 h and 36 h),the production of tyrosol increased significantly (P < 0.05),which of farnesol was also increased significantly (P < 0.05). In all,C. albicans produced most tyrosol at 24 h and most farnesol at 36 h. Conclusion:Tyrosol contributed to the biofilm formation by increasing the germ tube formation of the C. albicans biofilm. Farnesol inhibited the C.albicans biofilm formation by decreasing the germ tube formation. The inhibition of farnesol to the hyphae growth was more powerful than the contribution of tyrosol to the germ tube growth in the co-treated studies.

    • Effects of arachidonic acid on follicle failure of mice

      2014(9):1202-1205. DOI: 10.7655/NYDXBNS20140910

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      Abstract:Objective:To investigate the effect of arachidonic acid (AA) on follicle development of female mice and explore the relationship between AA and premature ovarian failure. Methods:ICR female mice of 4-week-old were fed with 10% AA. The weight of uterus and ovary,fatty acid analysis of ovaries,histological changes in ovaries were examined after four months. Results:The uterus and ovary weight of the 10% AA group were lower than those of the control group. Fatty acid analysis showed that the n-6/n-3 ratio of ovaries was increased in the 10% AA group. Under optical microscope,ovaries from the 10% AA group showed increased visible ovarian degenerative corpus luteum,interstitial fibrosis and increased proportion of atretic follicles. Conclusion:10% AA can cause obvious ovarian failure in mice.

    • A real-time tracking system for magnetic targeted drug:using magnetoresistive sensors

      2014(9):1206-1212. DOI: 10.7655/NYDXBNS20140911

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      Abstract:Objective:This desigh was based on magnetoresistive sensors, which realized real-time magnetic drug tracking. Methods:Magnetoresistive sensors detect magnetic field signals of the Fe-Fe3O4 core-shell structure nanoparticles which simulate magnetic drug. By conversion processing,spatial information of the magnetic particles can be obtained. Results:A virtual real-time detection system based on LabVIEW can display magnetic particles’ position. Conclusion:Experiments show that the system has good timeliness and accuracy,and can provide a new in vivo detecting approach of clinical studies on magnetic drug.

    • Associations between Periostin gene polymorphisms and heart failure in Chinese Han population

      2014(9):1213-1217. DOI: 10.7655/NYDXBNS20140912

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      Abstract:Objective:To determine the associations between Periostin gene polymorphisms and heart failure in a Chinese population. Methods:In total,464 patients with heart failure and 640 control individuals were included in this study. rs3829365 was genotyped through PCR and restriction fragment length polymorphism. Multivariate logistic regression was employed to analyze the independent strength of association among clinical parameters,genotypes and heart failure. Results:After adjusting for age,sex,hypertension,diabetes mellitus,smoking and hypertriglyceridemia in multivariate logistic regression,we found that CG or GG of rs3829365 was an independent risk factor for heart failure and that CG or GG of rs3829365 was significantly protective factors for heart failure in patients who smoked. Conclusion:These findings suggest that rs3829365 of Periostin gene may be helpful to determine the susceptibility and severity of heart failure.

    • A comparative study on the risk factors and the clinical features of coronary artery disease and coronary artery disease complicated with diabetes mellitus

      2014(9):1218-1223. DOI: 10.7655/NYDXBNS20140913

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      Abstract:Objective:To explore the differences of risk factors and clinical characteristics between coronary artery disease (CAD) complicated with diabetes mellitus (DM) and CAD. Methods:A total of 1 277 patients who were diagnosed with CAD by coronary angiography (CAG) from 1 926 patients were divided into the CAD without DM group and the CAD complicated with DM group.Meanwhile,649 patients with no CAD diagnosed from the 1 926 patients by CAG were considered as the control group. The clinical characteristics and the related risk factors of the subjects were collected. Results:Among the three groups,the distributions of the gender,age,BMI,fasting blood glucose (GLU),apolipoprotein A (apoA),lipoprotein a,hypertension,cardiac color ultrasound of LVEF (%),smoking status,daily smoking,drinking status,the intensity of work and the usual frequency of exercise were significantly different (P < 0.05). For the CAD with DM and without DM groups,BMI,GLU,apoA,daily smoking were significantly different (P < 0.05). Multivariate Logistic regression analysis showed that,compared with the control group,male,age > 60 y,hypertension,smoking were associated with the increased risk of CAD. Meanwhile,male,age > 60 y,BMI≥24 (kg/m2),high levels of TC and smoking had a significantly increased risk of CAD with DM. Compared with the CAD without DM group,female,BMI≥24 and high levels of TG were associated with the increased risks of CAD with DM. Conclusion:Female,BMI≥24,blood lipid abnormality and 3 lesions of coronary artery might be the risk factors for CAD with DM. Many measures should be carried out to release these risk factors and to improve the life quality of patients with CAD with DM and the prognosis of CAD with DM.

    • The predictive role of serum cholesterol and inflammatory factors on coronary plaque stability

      2014(9):1224-1227. DOI: 10.7655/NYDXBNS20140914

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      Abstract:Objective:To explore the correlation and prediction effect of serum cholesterol inflammatory factors on coronary plaque stability. Methods:Total of 107 patients with coronary heart disease were selected randomly in our hospital from July, 2013 to May, 2014,55 cases of stable angina pectoris patients (SAP) were set as the SAP group,52 cases of acute coronary syndrome (ACS)patients were set as the ACS group. Total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),total bilirubin(TBil),high sensitive C reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and other inflammatory factors were detected. Results:TC and LDL-C of the ACS group were(6.38 ± 1.17)mmol/L and(5.63 ± 1.12)mmol/L,respectively, higher than those of the SAP group[(4.51 ± 1.06) mmol/L and(4.42 ± 1.05)mmol/L,respectively],which were higher than those of the control group ,and the differences were significant(P < 0.05). TBil of the ACS group was(6.28 ± 0.74)μmol/L,and SAP was(9.96 ± 1.02)μmol/L,lower than those of the control group, and the differences were significant(P < 0.05). Hs-CRP,IL-6 and TNF-α of the ACS group were(19.06 ± 3.72)mg/L,(57.35 ± 4.14)pg/L and(38.11 ± 2.64)pg/L,higher than those of the SAP group[(11.31 ± 2.15)mg/L,(29.60 ± 3.08)pg/L and(26.82 ± 2.13)pg/L,respectively],and were higher than those of the control group,and the differences were significant(P < 0.05). Conclusion:It is indicated that serum TC,LDL-C,IL-6,TNF-α and Hs-CRP were increased in patients with coronary heart disease,and were correlative with coronary plaque stability.

    • Prevalence trend of adenocarcinoma of esophagogastric junction in a single institution in China

      2014(9):1232-1235. DOI: 10.7655/NYDXBNS20140916

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      Abstract:Objective:To investigate the veriation trend of the prevalence of adenocarcinoma of esophagogastric junction (AEG). Methods:The medical records of 4 685 cases diagnosed with gastric cancer in the First Affiliated Hospital of NJMU between 2001 and 2011 were reviewed and analyzed retrospectively. There were 1 824 cases of AEG and 2 861 non-esophagogastric junction adenocarcinoma (NEGA) among them. The veriation trend of proportion of AEG and early adenocarcinoma of esophagogastric junction (EAEG) as well as the distribution of age and gender were evaluated and the average age and the ratio of male to female were compared between the AEG and NEGA group. Results:The proportion of AEG in gastric cancer had not changed during the past 11 years,however,the proportion of EAEG was significantly higher between 2007 and 2011 than that in the period from 2001 to 2006 (12.23% vs 8.47%,P = 0.013). Compared with the NEGA group,patients in the AEG group had higher mean age [(62.7 ± 9.5)vs (59.1 ± 12.7)years,P < 0.001)]and male to female ratio (1 434∶390 vs 1 990∶871,P < 0.001). Conclusion:The prevalence of AEG in gastric cancer was not significantly increased,whereas the proportion of EAEG over the past 5 years was significantly increased. AEG occurs more often in elderly male patients.

    • The laboratory result analysis of mild-stimulation or natural cycle treatment in IVF/ICSI

      2014(9):1236-1239. DOI: 10.7655/NYDXBNS20140917

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      Abstract:Objective:To investigate the quality of oocyte and embryo developmental potential of poor ovarian responders with ovarian dysfunction,aged,ovulation induction contraindications,leading to repeated implantation failure by a conventional stimulation protocol for IVF/ICSI. Methods:The differences between minimal stimulation and natural cycle were compared in embryo development,embryo implantation,clinical pregnancy and outcomes. Results:A total of 2 565 cycles completed IVF/ICSI-ET treatment during January 2010 to November 2012 which includes 1 752 minimal stimulation cycles and 813 natural cycles. The ratios of normal fertilization rate,cleavage rate and good quality embryos all tend to increase with the increase of cycle number. Conclusion:For patients with aged,ovulation induction contraindications,ovarian dysfunction and patients with previous poor ovarian response or low high-quality embryo rate,mild stimulation or natural cycle might be an optimal choice.

    • The cerebral protection of carbon dioxide field flooding on open heart surgery with extracorporeal circulation

      2014(9):1240-1243. DOI: 10.7655/NYDXBNS20140918

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      Abstract:Objective:To explore the cerebral protection of carbon dioxide (CO2) field flooding on open heart surgery with extracorporeal circulation(ECC). Methods:From Jun 2012 to Mar 2013,97 patients undergoing open heart surgery with ECC were randomly divided into either CO2 insufflation(group Ⅰ,n = 50)or unprotected controls(group Ⅱ,n = 47). Blood samples were collected from the internal jugular vein at 5 min after anesthesia(T0),5 min(T1),1 h(T2),4 h(T3)and 24 h(T4)after release of the aortic cross-clamp,and the plasma concentrations of S-100β and neuronspecific enolase(NSE)were measured. The mini-mental state examination (MMSE)was performed to assess cognitive function before and 7 days after the operation. It was considered to be postoperative cognitive dysfunction (POCD)when the score decreased ≥ 1 standard deviation. Results:The plasma concentrations of S-100β at T1-T3 and NSE at T1-T4 in group I were higher than those at T0(P < 0.05). The plasma concentrations of S-100β and NSE at T1-T4 in group II were higher than those at T0(P < 0.05). Compared with group II,the concentrations of S-100β at T1-T4 and NSE at T2-T4 in Group Ⅰ were significantly decreased(P < 0.05). MMSE after surgery could not be performed in two patients of each group for reduced physical or mental status on the 7th postoperative day,respectively. The postoperative MMSE scores were significantly reduced in both groups compared with preoperative MMSE scores(P < 0.05),and the score in group Ⅱ was lower than that in group I(P < 0.05). Sixteen patients suffered POCD in group Ⅰ,and 25 cases in group Ⅱ,and the differences were significant(P < 0.05). Conclusion:It is very important to apply CO2 field flooding during open heart surgery,which can reduce the increasing extent of plasma S100β and NSE concentrations,and improve early postoperative cognitive function and cerebral protection.

    • Is it safe and feasible that the liver transplantation without routine prophylactic drainage?

      2014(9):1244-1247. DOI: 10.7655/NYDXBNS20140919

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      Abstract:Objective:To analyze the safety and feasibility in orthotopic liver transplantion without abdominal drainage. Methods:we retrospected drains in 175 patients who did liver transplantation in liver transplantation center from January 2010 to September 2013. Patients were divided into a drainage group (n = 63) and a non-drainage group (n = 112) separately according to the application of abdominal drainage. Preoperative factors,factors during operation,perioperative complications and lengths of stay were analyzed and compared. Results:Preoperative factors of patients showed no significant difference.(P > 0.05) No significant difference was noted in the blocking time of vena cava and portal vein,with bleeding and transfusion condition indicating no significant difference either. Furthermore,no significant deviation was noted regarding to complication grading (P = 0.78) and complications related to abdominal drainage (e.g. bleeding,bile leakage,massive ascites,incision drainage). However,significant deviation was noted in the two groups about lengths of stay in ICU and lengths of stay after operation (P < 0.01). Conclusion:It is feasible and safe for not using abdominal drainage after liver transplantation-

    • Huaiqihuang on IL-13 expression in airway and blood in asthma

      2014(9):1248-1252. DOI: 10.7655/NYDXBNS20140920

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      Abstract:Objective:To evaluate IL-13 concentration changes in serum of children with asthma and the effect of Huaiqihuang on asthmatic rats for expression of inflammatory factors and the effect with budesonide. Methods:Ten asthmatic children (the asthma group) and 10 healthy children (the control group) were selected. There was no significant difference in age,gender and body mass index between the asthma group and the control group. Serum IL-13 was measured by enzyme-linked immunosorbent assay (ELISA). Fifty healthy male SD rats (aged 4-6 weeks),after adaptive feeding for one week,were randomly divided into 5 groups:the normal control group,the asthma model group,the budesonide group (the BUD group),the Huaiqihuang group (the H group) and the budesonide + Huaiqihuang group (the BUD+H group). Asthma model was induced ovalbumin (OVA) sensitization and challenge for 6 weeks. Rats were analyzed for tissue biopsy. In addition,ELISA was used to detect the IL-13 in blood and BALF in both asthmatic and control subjects. Results:Serum IL-13 concentration in the asthma group was higher than in that in the control group. Pulmonary histopathological changes:inflammatory cell infiltration,columnar cell hyperplasia,trachea wall thickening were observed in the model group around the airway;the normal group had no such differences;the BUD group,the H group,the BUD+H group showed less inflammation cells infiltration than in the model,the BUD+H group reduced more than in the H group (P < 0.05). Compared to the normal group,in the model group, IL-13 concentrations in BALF and plasma increased (P < 0.05). Compared with the model group,in the BUD group,the H group and the BUD+H group,IL-13 concentration in BALF and plasma reduced to varying degrees (P < 0.05),and the BUD+H group changed more obvious than in the BUD group (P < 0.05). Conclusion:In the OVA-induced asthma model,the use of Budesonide,Huaiqihuang or Budesonide + Huaiqihuang combination therapy can improve airway inflammation in different degrees. Budesonide + Huaiqihuang combination therapy may have a synergistic effect.

    • Meta analysis of risk factors of congenital heart disease of Chinese perinatal children

      2014(9):1273-1278. DOI: 10.7655/NYDXBNS20140927

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      Abstract:Objective:To investigate the main risk factors of congenital heart disease in Chinese children so as to provide evidences for decision-making on prevention. Methods:The results of 26 studies on risk factors of congenital heart disease were analyzed by meta-analysis. The numbers of cumulative cases and controls were 7 917 and 12 835 respectively. The calculation methods of the merged odds ratio(OR)and 95% confidence interval(CI)were determined according to the homogeneity test. Results:The pooled OR values and 95% CI as follows:spontaneous abortion was 1.69(1.24~2.31);exposure to noise was 1.28(0.39~4.19); fetal abnormality was 2.31(1.25~4.27);medicine use in pregnancy was 1.88(1.57~2.23);exposure to damage chemicals in pregnancy was 3.46 (2.11~5.66);history of consanguineous marriage was 2.88 (1.88~4.40);passive smoking was 1.16 (0.69~1.95);adverse mental stimulus was 2.58 (1.36 ~4.91);father drinking was 1.88 (1.44 ~2.45);cold of early pregnancy was 3.06 (2.17~4.32). Conclusion:The risk factors of congenital heart disease are spontaneous abortion,fetal abnormality,medicine use in early pregnancy,adverse mental stimulus,father’s drinking,history of consanguineous marriage and had a cold during early pregnancy.

    • Thiopurine methyltransferase polymorphisms and thiopurine toxicity in treatment of acute lymphoblastic leukemia

      2014(9):1279-1283. DOI: 10.7655/NYDXBNS20140928

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      Abstract:Objective:To investigate the relationship between thiopurine methyltransferase (TPMT) polymorphisms and thiopurine-induced adverse drug reactions (ADRs) in 6-macraptopurine(6-MP)of acute lymphoblastic leukemia (ALL). Methods:Eligible articles that compared the frequency of TPMT polymorphisms among thiopurine-tolerant and intolerant ALL patients were included. Statistical analysis was performed with STATA 11.0. Sub-analysis/sensitivity analysis and bias evaluation were also performed. Results:Five studies that investigated a total of 426 participants met our inclusion criteria. The incidence of TPMT gene mutation was increased 4.55-fold (95% CI:1.92-10.80,P = 0.001) and 2.63-fold (95% CI:1.40-4.93,P < 0.003),respectively,in ALL patients with bone marrow toxicity (BMT) and thiopurine-induced hepatotoxicity,compared with controls. Conclusion:This meta-analysis suggests that the TPMT polymorphisms are associated with BMT and hepatotoxicity. We need father investigation based upen big sample to draw more realiable conclusions

    • Multiple regression analysis of quality of life for residents in Jiangsu province

      2014(9):1293-1296. DOI: 10.7655/NYDXBNS201409231

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      Abstract:Objective:To understand the situation of quality of life of residents in Jiangsu Province,and different regional residents’ quality of life,discuss the main factors which affect the quality of life of residents,to provide reference for the relevant decision-making. Methods:A self-administered questionnaire (GQOLI-74) was made by households interview over the age of 18 years residents,1 806 valid questionnaires were collected,and data was analyzed through analysis of variance and regression analysis. Results:The total score of quality of life of residents in Jiangsu province is 68.2 points,the average score of the residents in the central,southern and northern Jiangsu was respectively 69.0,69.6 and 66.3(P < 0.05);the average score of the urban residents(70.0) was higher than rural residents (67.1) (P < 0.05);the average score of male residents was 68.6,and female 67.7 (P > 0.05). The residents' cultural level was positively correlated with the quality of life score (P < 0.05). Multiple regression analysis showed that factors positively correlated with quality of life were physical discomfort,self-esteem,marriage and family,motor and sensory function;negatively correlated with negative emotion. Conclusion:The differences of residents’ quality of life between different regions,urban and rural areas,different cultural levels were significant,while no difference found between sexes. Measures should be taken to improve the level of quality of life of residents in Jiangsu Province.

    • A Meta analysis of the efficacy and safety of endoscopic orbital decompression for Graves’ ophthalmopathy

      2014(9):1284,1288-1292. DOI: 10.7655/NYDXBNS20140929

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      Abstract:Objective:To assess the efficacy and safety of endoscopic orbital decompression systematically in treating Graves’ ophthalmopathy. Methods:Databases included the Cochrane Central Register of Controlled Trials,Medline from their inception to May,2013,and the relevant journals and references of articles were also retrospectively searched to collect randomized controlled trials (RCTs) or before-after self-controlled trials on endoscopic orbital decompression. Two reviewers independently screened the articles according to the predefined inclusive and exclusive criteria,and then extracted necessary data and evaluated quality of the included studies. Then meta-analysis were performed using RevMan 5.1.0. Results:A total of the 5 self-controlled trials involving 156 patients were finally included. The results of meta-analysis showed a significant reduction in proptosis without marked change in diplopia. However, the sight and the incidence of diplopia did not change significantly. Sensitivity analysis indicated that these results were stable. Conclusion:Endoscopic orbital decompression improved proptosis and ophthalmological function in Graves ophthalmothy. There is no evidence that endoscopic orbital decompression could increase the risk of new upset diplopia. However,prospective RCTs with larger scale and longer follow-up are still necessary to confirm this conclusion.