Abstract:Objective:To study the dynamic expression of male sterility related gene Boule in the first spermatogenesis wave of mouse testes as well as the function of Boule during spermatogenesis. Methods: The expression level of BOULE protein in various tissues and time points were examined using Western blotting. Real-time PCR was used to establish the Boule mRNA expression profile after birth. Immunohistochemistry and immunofluorescence were carried out to analyze the expression and distribution of BOULE protein in mouse testes. Results: Western blot confirmed the specific expression of BOULE protein in mouse testes. Real-time PCR showed that Boule mRNA was first detectable in the testis of mice 20 days after birth. Immunohistochemistry showed that BOULE protein located in the cytoplasm of pachytene spermatocyte and round spermatid during spermatogenesis. Immunofluorescence witnessed the BOULE granule,which formed in the cytoplasm. Conclusion: Boule expression is precisely regulated during spermatogenesis. The specific expression in the cytoplasm of spermatocyte and round spermatid suggested that Boule may be important for spermatocyte progressing and round spermatid differentiation.

Abstract:Objective:To explore the effects of gp120 on TEA sensitive K+ currents and the role of the currents in gp120 induced neuronal apoptosis. Methods: Hippocampal neurons were harvest from 18-day-old embryonic rats and divided into two groups: the control and the gp120 treated group. The outward delayed K+ currents were recorded by performing the whole cell patch clamp, subsequently the cell viability as well as neuron apoptosis were evaluated by MTT and TUNEL assay,respectively. Moreover, the protein expression of Kv2.1, a subtype of TEA sensitive K+ channel, was detected by Western blot. Results: Gp120 significantly increased the outward K+ currents in a dose-dependent manner, and the TEA sensitive K+ currents were participated in K+ currents increment mediated by gp120. The MTT and TUNEL results revealed that gp120 substantially decreased the cell viability and enhanced the cell apoptosis, while the K+ antagonist TEA was effectively decreased gp120 induced cell damage. Moreover, the expression and contribution of Kv2.1 on TEA sensitive K+ currents was significantly up-regulated by gp120. And the specific blocker, GxTX-1E, was used for evaluating the role of Kv2.1 in TEA sensitive K+ currents. Conclusion: TEA sensitive K+ currents were involved in gp120 induced neuronal damage, and the subtype Kv2.1, which was up-regulated by gp120, might exert effects on gp120 induced cell damage.

Abstract:Objective:To investigate the protective effect and possible mechanism of whisker stimulation on focal barrel cortex ischemia in rats. Methods: Eighteen male Sprague-Dawley rats, which had been trained to reach criteria by completing a whisker-dependent task, were randomly divided into groups of sham-operation, operation and whisker stimulation. The focal ischemia model of whisker-barrel cortex was established by ligating 2~3 branches of the right middle cerebral artery permanently under microscope. The left side whiskers of the whisker stimulation group were stimulated 3 days after ischemia. The vibrissal function of the three groups was evaluated each day after ischemia until rat reached the criteria again. Meanwhile, we record the needed numbers of trials for complete the task. Local cerebral blood flow was assessed by laser Doppler scanner, brain tissue changes were observed with HE staining, the expression of CD34 and microvessel density (MvD) was detected with immunohistochemistry method 14 d after ischemia. Results: Compared with the operation group, the whisker stimulation group significantly decreased the number of trials that required to reach criteria (P < 0.01), enhanced local cerebral blood flow (P < 0.01), detained the pathologic change of ischemic brain, increased the numbers of CD34 positive cells and also MvD (P < 0.05). Conclusion: Whisker stimulation had protective effects on focal barrel cortex ischemia in rats. One possible machanism may be that whisker stimulation can increase expression of CD34 and enhance local cerebral blood flow.

Abstract:Objective:To explore whether miR-335 has its own transcription unit and effects of rosiglitazone and insulin on transcriptional activity of MiR-335 promoter regions. Methods: We cloned different fragments from upstream sequences of miR-335 in MEST intron by PCR. Then, the luciferase activity of these different plasmids was detected by dual-luciferase reporter system. After the stimulation of 1 μmol/L rosiglitazone and insulin of different concentration, the transcriptional activity change of fragments from upstream sequences of miR-335 was examined. Results: Different miR-335 promoter regions had different transcription activity, and the region of about 600 basic groups in miR-335 upstream exhibited the highest activity. The activity of miR-335 promoter in untreated HEK293T cells showed no statistical significance compared to the baseline value. The levels of miR-335 promoter activity showed significant elevation after the intervention of 1 μmol/L rosiglitazone (P < 0.01). The activity of miR-335 promoter was a little elevated with the stimulation of 10-12 mol/L insulin (P < 0.05). Meanwhile, the levels of miR-335 promoter activity had no statistical significance when the concentration of insulin increased. Conclusion: About 600 basic groups in miR-335 upstream were major promoter regions. Meanwhile, miR-335 may play an important role in the development of obesity and insulin resistance via its own transcription mechanism.

Abstract:Objective:This study investigated the expression and significance of both P-cadherin and HSP27 proteins in gastric carcinoma tissues as well as the relationships among the clinical pathologic factors, and discussed the meaning of being prognostic indicators. Methods: High throughput technology of tissue microarray and immunohistochemical SP method was employed to investigate the expression of P-cadherin and HSP27 proteins in 75 gastric carcinoma specimens from patients without chemo-radiation therapy and 75 non-tumor gastric tissues. Survival analysis was carried out on the follow-up of patients. Results: In gastric cancer tissue and normal gastric tissue, the expression rates of P-cadherin, HSP27 were detected to be 40.0% and 82.7%, 64.0% and 30.7%, respectively (both P < 0.001). High expression of P-cadherin and low expression of HSP27 were related to clinical stage, differentiation degree and lymph node metastasis (P < 0.05), the high expression of HSP27 was also correlated with tumor size (P <0.05). According to the results of Pearson correlation analysis, the expression rates of P-cadherin and HSP27 in gastric cancer were negatively correlated to each other (P < 0.05). Kaplan-Meier analyses showed that P-cadherin and HSP27 were significantly associated with the prognosis (P < 0.05). The results of univariate analysis of Cox showed that P-cadherin, HSP27, clinical stage, tumor size and lymph node metastasis were significantly correlated with the prognosis (P < 0.05); The multivariate analysis of Cox model indicated that HSP27 and clinical stage were significantly correlated with the prognosis (P < 0.05). Conclusion: The low expression of P-cadherin in gastric carcinoma and high expression of HSP27, which was closely related with the clinical pathological features and biological behaviors of gastric cancer. Expression of P-cadherin and HSP27 were negatively correlated in gastric carcinoma. P-cadherin and HSP27 could be used as the candidate index to judge the prognosis of patients with gastric carcinoma.

Abstract:Objective:To prepare a fibronectin (FN) modified poly(lactic-co-glycolic acid) (PLGA) membrane via dopamine (DP), and evaluate the effects of FN on cell adhesion of ME3T3-E1. Methods: Attenuated total reflectance Fourier transform infrared spectrometer (FTIR), contact angle meter and quartz crystal microbalance with dissipation were performed to measure the surface groups, contact angle and FN loading of the samples; Time lapse imaging system was performed to observe cell adhesion of ME3T3-E1 every 3 min, and cell counting Kit-8 was used to measure the cell adhesion 0.5, 1.0, 4.0, 8.0 h after cell vaccination in phosphate buffered saline (PBS). Results: FTIR and contact angle showed DP was successfully grafted onto PLGA, and improved hydrophilicity was acquired on DP modified PLGA surfaces. Quartz crystal microbalance with dissipation showed that FN loading was 22.5 ng/cm2. Higher cell adhesion ratio was observed with time lapse imaging system and CCK-8 tests in FN loading samples than in PLGA and PLGA-DP membranes (P < 0.05). Conclusions: MC3T3-E1 can adhere to FN modified PLGA membranes via DP in PBS successfully.

Abstract:Objective:To investigate the beneficial effects and the potential molecular mechanisms of Berberine on cardiac hypertrophy induced by pressure overload in rats. Methods: Transverse aortic constriction (TAC) surgery was conducted to set cardiac hypertrophy model in rats. There were three groups: the sham group (n=8), the TAC group (n=8),and the TAC + Berberine group (n=8). Echocardiography was performed to evaluate the cardiac function 4 weeks after the TAC surgery. Histopathology staining, Masson trichrome staining, TUNEL staining, and other molecular biology methods were performed to measure myocardial cell size, cardiac fibrosis, myocardium cell apoptosis, and other indexes. The mRNA expression of atrial natriuretic factor (ANP) was detected by RT-qPCR. To evaluate endoplasmic reticulum (ER) stress level, Western blotting was carried out to test the expression of ER stress associated proteins Bip/GRP78 and CHOP. Results: Compared with the TAC group, Berberine significantly improved cardiac hypertrophy situation (P < 0.05), reduced myocardial interstitial fibrosis and cardiac apoptosis (P < 0.05). Western blotting showed that Berberine administration remarkably decreased the expression of Bip/GRP78 and CHOP induced by TAC surgry (P < 0.05). Conclusion: Berberine could protect hypertrophic myocardium by attenuating TAC-induced cardiac hypertrophy and dysfunction and inhibiting myocardial cell apoptosis, and that cardioprotective effect of Berberine is, at least in part, associated with reducing endoplasmic reticulum stress-meditated apoptosis.

Abstract:Objective:To investigate the level of cardio-specific microRNA-499 levels (miR-499) in acute myocardial infarction (AMI) patients and to explore the effect of plasma miR-499 levels on the diagnosis of AMI. Methods: The subjects enrolled in this study, included 30 healthy subjects and 73 patients with suspected acute coronary syndrome (ACS),who went on our hospital in 2013 and were divided into the AMI group and the unstable angina (UA) group with 53 and 20 patients, respectively. MiR-499 concentrations were measured with a real-time reverse-transcription PCR (qRT-PCR). Blood samples of the AMI group were collected 12 h and 24 h after the onset of symptom, and those of the UA group were collected after admission. Serum cardiac troponin I (cTnI ) and creatine kinase-MB (CK-MB) concentrations were measured by the electrochemiluminescence method. The 53 AMI patients were further divided into various subgroups according to coronary arteries involved and primary PCI or not, plasma miR-499 concentrations were measured by TaqMan real-time PCR and analyzed between the two groups. Results: The relative level of miR-499 was significantly increased in patients with AMI (4.57 ± 2.3) than that of UA subjects (2.75 ± 1.39) and normal subjects (0.5 ± 0.39) (both P < 0.01). The serum miR-499 relative level in the patients with AMI had a positive correlation with serum cTnI or CK-MB (r = 0.361, 0.428, respectively, both P < 0.01). It was significantly higher in 32 AMI patients with double or triple coronary arteries stenosis than those with single stenosis (P < 0.01). MiR-499 was significantly decreased in 24 patients after received primary PCI (P < 0.01). Conclusion:The plasma concentration of miRNA-499 with cardiac specific expression is low in healthy person, but significantly increase after myocardial infarction and can be detected in the early stage. This can be used for early diagnosis and AMI severity evaulation. The plasma concentration of miR-499 may be a useful biomarker of AMI in humans.

Abstract:Objective: To evaluate the safety and immunogenicity of booster immunization of adsorbed tetanus vaccine developed by Olymvax Biological Technology co., LTD in adults. Methods: This study was divided into two phases. For the first phase, openly design was adopted to evaluate the safety of experimental vaccine and the second phase would be performed if the vaccine was confirmed to be safety. The second phase of this study was designed to be a single center, randomized, double-blinded, paralleled controlled clinical trial. Health residents aged 18 to 30 years old were recruited to receive one dose of experimental adsorbed tetanus vaccine and control vaccine. Daily diary was used to collect safety data. Blood samples were collected right before and 28 days after vaccination for Tetanus toxoid antibody detection through standard enzyme linked immunosorbent assay. Results: Thirty participants were enrolled in the first phase of clinical trial. Frequency of total adverse reactions (AR) observed within 7 days after vaccination was 33.3%. In the second phase of clinical trial,1 200 participants were enrolled with 600 participants in experimental vaccine and control group, respectively. Incidences of total AR were 30.7% and 31.5% for vaccine and control group, respectively. Most common injection-site AR was pain, while most common systemic AR was fever. No statistically significant differences of frequencies of AR were found between treatment groups. For both group, good immunological response was induced, with proportion of participants with antibody concentration ≥0.1 U/ml for both groups up to 100% and GMCs were 3.38 U/ml and 3.16 U/ml, respectively. Conclusion: Adsorbed tetanus vaccine developed by Olymvax Biological Technology co., LTD showed good safety and immunogenicity, which is applicable for booster immunization for adults, and for further developing of combined vaccine.

Abstract:Objective: To observe the clinical effects of bailing capsule on pulmonary function, airway inflammation and oxidative stress in patients with moderate to severe chronic obstructive pulmonary disease (COPD). Methods: Sixty patients with moderate to severe COPD were randomly divided into two groups: the control group and the treatment group. Every group contained 30 patients. The control group was treated with symbicort turbuhaler,and the treatment group was treated with symbicort turbuhaler plus bailing capsule. The pulmonary function and the inflammatory cells classification in induced sputum were measured before and after treatment (24 weeks). Serum superoxide dismutase(SOD), malondialdehyde (MDA) and total antioxidant capacity (TAOC) in serum were detected in the two groups. Results: Forced expiratory volume in 1 second (FEV1), FEV1% and FEV1/forced expiratory volume (FEV1/FVC%) were significantly improved after treatment in the control group and the treatment group compared to pretreatment (all P < 0.001), and all were better in the treatment group than that of the control group (all P< 0.05); The percentage of neutrophils was decreased and the percentage of macrophages was increased in induced sputum after treatment in both groups compared with pretreatment (all P < 0.001), and the changes are more significat in the treatment group compared to the control group (all P < 0.001); The levels of SOD and TAOC were enhanced, and level of MDA was reduced after treatment in the control group and treatment group compared to pretreatment (all P < 0.05), and the changes are more remarkable in. the treatment group compared to the control group (all P < 0.001). Conclusion:Bailing capsule could improve pulmonary function, reduce airway inflammation and inhibit oxidative stress in COPD patients.

Abstract:Objective:We applied fluorescein fundus angiography (FFA) to analyze diagnostic criteria of fibrovascular proliferative membranes of diabetic retinopathy (DR) Ⅳ~Ⅴ period, and to observe different parts, shape, and the effects of different sizes of fibrovascular membrane photocoagulation. Methods: A total of 98 eyes from 56 patients with DR Ⅳ~Ⅴ period were analyzed in this study. FFA was performed to evaluate the above-mentioned 56 cases in the general situation of the retina and specified the panretinal photocoagulation treatment. During follow-up of 6 months, the patients were examined by FFA and routine inspection in the 1st, 3rd and 6th months, respectively. According to treatment effects, the 56 cases were divided into two groups:the effective treatment group and the ineffective treatment group. The result of pre-treatment FFA was analyzed and different parts, shapes as well as the treatment effect of different sizes of fibrovascular membrane photocoagulation were observed. Results: A total of 98 eyes from 56 patients were selected. The effective treatment group included 72 eyes and the ineffective treatment group included 26 eyes. The efficiency was 73.5%. There were 18 eyes undergoing vitrectomy and 2 eyes became neovascular glaucoma. According to the results of pre-treatment FFA, we compared and observed the fibrovascular membrane of the two groups. There was a significant difference of angiogenesis of merged optic disc between the two groups (P < 0.05). Furthermore, there was a significant difference of fibrovascular membrane range between the two groups (P < 0.05). Also, the difference of diffuse film membrane and bridge-shaped membrane between the two groups was significant (P < 0.05). Conclusion: The analysis of different parts, shapes and sizes of fibrovascular membranes by FFA has the guiding significance to panretinal photocoagulation treatment and vitrectomy.

Abstract:Objective:To invertigate the clinical applying value of microarray comparative genomic hybridization (array-CGH) technology for the preimplantation genetic diagnosis (PGD) of chromosomal translocation carriers. Methods: Using the array-CGH technology to reanalyze 15 blastocysts of chromosomal translocation carriers,which was diagnosed abnormality by the fluorescence in situ hybridization (FISH) technology, then establish and clinically apply the array-CGH PGD for chromosomal balanced translocation carriers. Results: A total of 15 blastocysts diagnosed abnormality by FISH technology was amplified by whole genome amplification (WGA), and then was detected by array-CGH technology. Array-CGH could not only detect numerical and structural chromosomal abnormalities in accordance with FISH results, but also found other chromosomal abnormalities outside translocation chromosomes. The breakpoint position between translocation chromosomes detected by array-CGH technology was the same with its peripheral blood chromosomal karyotype results. Five cycles of PGD were carried out for chromosomal translocation carriers with array-CGH technology. One euploid embryo was transferred back to the woman＇s uterus, which resulted in successful implantation and pregnancy, whose karyotype of fetal amniotic fluid chromosomal was normal. Conclusion: WGA combined with array-CGH could comprehensively access the embryo＇s chromosomes of chromosomal translocation carriers, so it has good clinical applying prospects.

Abstract:Objective:To study 99Tcm-MPO ([99mTc N (MPO) (PNP5)+: 2-mercapto pyridine N-oxide and N-ethoxy ethyl-N, N-two [2 (two (3-methoxy propyl) phosphine) ethyl] amine) biodistribution in normal mice,and to study the biodistribution in vivo and its application as a new myocardial perfusion imaging tracer on the clinical application value. Methods:99Tcm-MPO was synthesized in two steps, and HPLC method was performed to analyze the radiolabeling rate and in vitro stability of 99Tcm-MPO. The normal Kunming rats as experimental group received injection of 99Tcm-MPO 3.7 MBq via the tail vein, while the control group was injected with 99Tcm-MIBI 3.7 MBq, and the biodistribution (%ID/g) in dissected organs were measured respectively at 5, 15, 30, 60 and 120 min post-injection (p.i.). Two normal New Zealand rabbits were intravenously injected with 99Tcm-MPO and 99Tcm-MIBI 37MBq at the ear margin, and static images were performed by SPECT at 5, 15, 30, 60 min p.i., respectively. We respectively proceed SPECT/CT imaging at 15 and 60 min p.i. after blocking blood flow with a balloon in the experimental miniature pigs＇ coronary artery left circumflex branch. Results:99Tcm-MPO had high radiolabeling yield of (98.8 ± 1.0)% and radiochemical purity after 12 h of (96.5 ± 0.8)% in vivo. In vivo biodistribution study demonstrated that uptake of 99Tcm-MPO in the kidneys was similar to 99Tcm-tetrofosmin at 5 min p.i.. The uptake of 99Tcm-MPO in liver was(30.38 ± 0.43)% ID/g at 5 min, slightly more than 99Tcm-MIBI and 99Tcm-tetrofosmin, but 99Tcm-MPO has a faster liver clearance. The uptake of 99Tcm-MPO in the heart was (9.38 ± 0.70)%ID/g at 15min p.i., slightly lower than 99Tcm-MIBI, but there was no significant difference. The factor that 99Tcm-MPO has a significantly higher heart/liver ratios than 99Tcm-MIBI and 99Tcm-tetrofosmin at 15min p.i. (heart/liver ratios: 2.21 ±0.44 vs. 0.62 ± 0.02 for 99Tcm-MIBI and 0.89 ± 0.06 for 99Tcm-tetrofosmin, F=22.29, P=0.016). The rabbits planar imaging after intravenous injection 99Tcm-MPO showed visible images of the heart and liver at 5 min p.i., and the radioactivity uptake in liver significantly reduced at 15 min p.i.. 99Tcm-MPO has higher heart-liver ratios than 99Tcm-MIBI and 99Tcm-tetrofosmin at 15min p.i. (heart-liver ratios: 0.85 ±0.32 vs. 0.71 ± 0.15 for 99Tcm-MIBI and 0.59 ± 0.64 for 99Tcm-tetrofosmin), but there was no significant difference. SPECT/CT showed decreased uptake of 99Tcm-MPO by lesions with ischemia and reperfusion injury in porcine models, and there was no significant difference. Conclusion:99Tcm-MPO is a new myocardial perfusion imaging tracer. Compared with 99Tcm-MIBI,99Tcm-MPO showed higher uptake in heart, longer myocardial radioactivity retention time, and quicker liver clearance. The heart-liver radioactivity uptake was higher than 99Tcm-MIBI and 99Tcm-tetrofosmin at 15min p.i.. 99Tcm-MPO may be used for early myocardial perfusion imaging, and has a good prospect of clinical application.

Abstract:Objective:To identify the etiologic factors associated with palatally impacted canines and buccally impacted canines in a Chinese population using the cone-beam computed tomography (CBCT) technique. Methods:CBCT images of 262 Chinese subjects with impacted maxillary canines(buccally impacted canines and palatally impacted canines) and 262 age- and sex-matched subjects without impaction were admitted to the study. All CBCT records were analyzed in software programs for quatitative and quantitative variables of the teeth,dental arch and skeletal components by one rater. The differences among groups were compared statistically (SPSS 13.0 software). Results:The mesiodistal dimension of the lateral incisor was significantly smaller in the palatally impacted canine group than that in the other groups(P < 0.001). Both anterior maxillary dental width and skeletal width in the buccally impacted canine group were significantly smaller than those in the control group (P < 0.001). The groups with palatally impacted and buccally impacted canines had significantly increased prevalence of peg-shaped lateral incisors and incisor impaction,respectively(P < 0.001). The average mesiodistal location of the canine cusp tip was significantly different between the buccally impacted canines and the palatally impacted canines groups,being mesial and distal to the lateral incisor long axis,respectively. Conclusion:In Chinese subjects,buccal canine impaction is mostly associated with anterior transverse(dental and skeletal) deficiency and incisor impaction,however,palatal impaction is mostly associated with small or missing lateral incisors,consistent with the guidance theory. A mechanism is proposed to explain the processes leading to different fates of canine eruption in response to varied local factors.

Abstract:Objective:To evaluate postoperative effect and clinical efficacy on minimally invasive transforaminal lumbar interbody fusion (MIS TLIF) and open transforaminal lumbar interbody fusion (Open TLIF) in treatment of one-level lumbar spondylolisthesis diseases. Methods: The papers on one-level lumbar spondylolisthesis diseases published before March 2014 in database of PubMed, Embase, Cochrane library, CNKI, CBM, VIP were systematically retrieved. For controlled trials, prospective cohort study and retrospective cohort study on the comparison between minimally invasive and traditional open transforaminal lumbar interbody fusion in treatment of one-level lumbar spondylolisthesis diseases. Revman 5.2 was used for researching and systematically analyzing the clinical indexes of MIS TLIF and Open TLIF, which include operation time, intraoperative blood loss, intraoperative and postoperative complications and the last follow-up fusion rate. Results: Through the initial screening, secondary screening and further screening, 8 studies (with 2 randomized controlled studies and 6 cohort studies) involving 866 cases were included for the analysis, with 417 subjects in the MIS TLIF group and 449 in the Open TLIF group. The operation time, intraoperative and postoperative complications, the last follow-up fusion rate, preoperative VAS and ODI scores in the MIS TLIF group showed no statistical difference compared with the Open TLIF (P > 0.05); however, intraoperative blood loss, hospital stay, last follow-up ODI and VAS scores showed statistical difference (P < 0.05); Conclusion: Compare with Open TLIF, MIS TLIF did not increase the operative time, intraoperative and postoperative complications, and the long-term rate of fusion. In addition, MIS TLIF can reduce the duration of hospitalization and intraoperative blood loss, and meanwhile, early relieve postoperative pain and help functional recovery. These information indicates that MIS TLIF is an ideal treatment for one-level lumbar spondylolisthesis disease.

Abstract:Objective:To investigate the relationship between miR-23b expression abnormalities and developmental defects of the heart. Methods:Human and developing mouse embryo myocardial tissues were collected. P19 cells,induced to differentiating into cardiomyocytes with 0.9% dimethyl sulfoxide (DMSO),were collected at day 0,4,6,10. MiR-23b expression level of the mentioned tissues or cells was then detected. Online databases were used to predict target genes of miR-23b and the signaling pathways that the target genes may be involved in,thus to comprehensively analyze the relationship between miR-23b expression abnormalities and developmental defects of the heart. Results:For human embryos of first and second trimester of gestation,miR-23b expression was upregulated 4.4 times and downregulated 3.5 times,respectively,in the VSD (ventricular septal defect) group,compared with the normal control group. For mouse embryos,expression of miR-23b increased gradually at the four time points:d12.5,d14.5,d16.5,d18.5. Differences among the later three time points (after d14.5) were significant (P < 0.05). During differentiating into cardiomyocytes,P19 cells showed a descending trend in miR-23b expression. Differences between d4 and d6 or d6 and d10 were significant (P < 0.05). Bioinformatics about miR-23b suggests that it may be involved in TGF-β,Notch,Wnt signaling pathways. Conclusion:MiR-23b expression abnormalities may influence proliferation,apoptosis,migration and differentiation of cardiomyocytes and thus result in developmental defects of the heart.

Abstract:Objective:To determine the effect of triptolide on the proliferation and apoptosis of HL-60 cells in acute myeloid leukemia and Jurkat cells in T-cell acute lymphoblastic leukemia. Methods: Human leukemia cell lines were treated with triptolide at different concentrations. The inhibitory rate of cells proliferation was detected by MTT assay, apoptosis rate was detected by AnnexinV-FITC/PI double staining, cell cycle was observed by PI staining, and cell morphology was observed by transmission electron microscope (TEM). Results: Cell proliferation of HL-60 and Jurkat cells was significantly inhibited and their survival rates were reduced by triptolide in a dose-dependent manner, with the IC50 value for 24 h of (42.50 ± 9.38) nmol/L and (60.91 ± 9.77) nmol/L, respectively. Triptolide induced cells apoptosis in a dose-dependent manner,apparent cell cycle and typical apoptotic morphological changes. Conclusion: Triptolide could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat.