Abstract:Objective:To construct luciferase reporter plasmids of full-length and truncated promotors of rat response gene to complement 32 (RGC32) gene and detect their activity in HEK293 cells in response to myeloid zinc finger gene 1 (MZF1) overexpression,screening the possible binding sites for MZF1. Methods:Rat RGC32 promoter(-686~-1 nt) was amplified by PCR and cloned into the luciferase reporter plasmid (pGL3-basic). The recombinant plasmid (pGL3-RGC32-FL) and rat MZF1 expression plasmid (pIRES2-EGFP-MZF1) were co-transfected into HEK-293 cells and then the luciferase activity was detected to determine the role of MZF1 in RGC32 gene transcription. Meanwhile,the potential MZF1 binding sites within RGC32 promoter were predicted by bioinformatics software. Based on the predicted results,different luciferase reporter plasmid of truncated MZF1 gene promotor that named pGL3-RGC32-1,pGL3-RGC32-2 and pGL3-RGC32-3 were constructed. The promoter luciferase reporter plasmids of pGL3-RGC32-FL or pGL3-RGC32-1,pGL3-RGC32-2,pGL3-RGC32-3 and the plasmid of pIRES2-EGFP-MZF1 were co-transfected into HEK293 cells. Then,the luciferase activity was detected to screen the MZF1 binding sites. Results:It was verified that different kinds of plasmids were all constructed correctly by PCR analysis and nucleotide sequencing. The plasmids of pGL3-RGC32-FL and pIRES2-EGFP-MZF1 were also co-transfected into HEK293 cells,and then the luciferase activity was detected. The results showed that the transcriptional activity of RGC32 gene was increased markedly in response to MZF1 overexpression. In addition,the plasmids of pGL3-RGC32-FL or pGL3-RGC32-1,pGL3-RGC32-2 and pGL3-RGC32-3 and pIRES2-EGFP-MZF1 were co-transfected into HEK293 cells,and then the luciferase activity in different groups was determined. The result displayed that the activity of pGL3-RGC32-3 was much lower than that in pGL3-RGC32-FL,pGL3-RGC32-1 and pGL3-RGC32-3,indicating that the region of rat RGC32 promoter (-286~-86 nt) might contain MZF1 binding element. Conclusion:The rat full-length and truncated rat RGC32 promotor luciferase reporter plasmids were constructed successfully,overexpress MZF1 could increase the transcriptional activity of RGC32 gene,and the MZF1 binding region was identified.

Abstract:Objective:To investigate effects of estradiol(E2) on T lymphocytes in angiotensinⅡ (AngⅡ)-induced hypertrophy in mice. Methods:Eight-to 10-week-old female C57BL/6 mice suffered ovariectomy were randomly divided into experiment group (E2+AngⅡ) and control group(Placebo+AngⅡ). At the beginning,E2 sustained release tablets (0.25 mg/60 d) were implanted into the experiment group and placebos were implanted into the control group. Four weeks after E2/placebo implantation,Osmotic mini-pumps containing AngⅡ [1000 ng/(kg-min),4 weeks] were implanted subcutaneously in both groups and induced hypertrophy. During the whole experiment,blood pressure was measured at the end of 1th,2th,4th,6th,8th week. At the end of 4th and 8th week,echocardiography was performed to measure interventricular septum (IVS),left ventricular interior diameter (LVID),left ventricular posterior wall (LVPW) at diastole and calculate ejection fraction (%EF) and fractional shortening (%FS). At the same time,we analyzed immunologic markers on T cells from mice in groups by multicolor flow cytometry (Canto Ⅱ,BD). Heart morphologic changes were detected by HE staining at the end of 8th week. Results:Blood pressure in E2+AngⅡ group was lower than that in Placebo+AngⅡ group at the end of 6th,8th week (P < 0.05). IVS and LVPW were alleviated in E2+AngⅡgroup than that in Placebo+AngⅡgroup. Before giving Ang II,the fraction of total T lymphocytes,CD4+T cells,CD8+T cells and the ratio of CD4+ and CD8+ were not statistically significant. Giving AngII for 4 weeks,the fraction of circulating total T lymphocytes cells and CD8+T cells in E2+AngⅡ group were lower than that in Placebo+AngII group (P < 0.05). The fraction of circulating CD4+T cells and the ratio of CD4+ and CD8+ in E2+AngⅡ group were higher than those in Placebo+AngII group (P < 0.05). Conclusion:E2 alleviated hypertrophy induced by chronic AngⅡ. During the process of hypertrophy,E2 could decrease the fraction of total T lymphocytes cells and CD8+T cells,and increase the fraction of CD4+T cells and the ratio of CD4+ and CD8+. Our research indicated that E2 could affect T cells population to alleviated hypertrophy induced by AngⅡ.

Abstract:Objective:To investigate the expression and significance of β-catenin in different times of synovial tissue of osteoarthritis SD rat models. Methods: Ninty SD rats were divided into experimental and control groups randomly. The anterior cruciate ligament(ACL) was cut off to establish a experimental model,the treatment of control group was similar to the experimental group,transction ACL. Rats were sacrificed at 4,8 and 12 weeks after surgery,the synovial tissue wore obtain,and the expression of protein and gene of β-catenin was measured by immunohistochemistry (IHC) and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR)in different times of experimental groups and control groups. Results: Results from IHC showed that β-catenin was expressed in all experimental rats,and the expressions of β-catenin 4/8/12 weeks were different (all P < 0.05). The expressions of β-catenin in 4 and 8 weeks were not different in control groups,and the expression of β-catenin was positive in part of the control group in 12 weeks. Semi-quantitative RT-PCR showed that the expression of β-catenin in the experimental group was higher than that of the control group,and the expression increased with the extension of time in the experimental group,and the expression of β-catenin of control groups were different 4/8/12 weeks among (P < 0.05). Conclusion:β-catenin was expressed in the synovial tissue of OA at a high level. And its level was increased with the extension of time,which indicates that. β-catenin plays a critical role in the pathogenesis and progression of osteoarthritis.

Abstract:Objective:To investigate the effect of carboxymethytl pachymaram(CMP) on inhibiting apoptosis of human monocyte-derived dendritic cells in vitro. Methods:Human dendritic cells were induced from the peripheral blood monocytes in vitro using recombined human GM-CSF and interleukin (IL)-4,and culture in the presence of CMP at different concentrations (10,50,and 100 mg/L). The percentage of apoptosis and phosphorylated Akt proteins in mature DCs was measured in the presence or absence of CMP by flow cytometry and Western blot,respectively;The expression of CCR7 in mature DCs was measured in the presence or absence of CMP by flow cytometry and Q-PCR,respectively. Results:In CMP-induced DC, the percentage of apoptosis was significantly lower(in the presence of 100mg/L CMP) and the expression of CCR7 and phosphorylated Akt protein significantly higher(in the presence of 50mg/L and 100mg/L CMP) than that of control group,both in a dose-dependent model. Conclusion:CMP can inhibit the apoptosis of human monocyte-derived dendritic cells and its anti-apoptotic mechanism is probably mediated by CCR7 and PI3K/Akt signaling pathway.

Abstract:Objective:To construct lentiviral vector of recombinant BTLA gene and investigate the effects on proliferation of mice splenic T lymphocytes after overexpression of BTLA gene mediated by lentiviral vector. Methods:The BTLA gene was cloned into the lentiviral expression vector (pWPTS-GFR),which was verified by reverse transcription and PCR. The lentiviral vector of recombinant BTLA gene was co-transfected into 293T cells. The morphological changes of 293T cells infected by lentiviral granules were observed by fluorescence microscope and lentivirus titer was tested by the method of TCID50. Results:pWPTS-mBTLA lentivrial vector at a titer of 1.3×108 pfu /mL was successfully constructed. Overexpression of BTLA gene in mice splenic T lymphocytes significantly suppressed the activation and proliferation of mice splenic T lymphocytes on day 4 and 8(P < 0.05). Conclusion:Outcomes of the inhibiting effect in activation and proliferation of mice splenic T lymphocytes induced by overexpression of BTLA gene indicated the successful construction of lentiviral vector of the recombinant BTLA gene.

Abstract:Objective:In our previous study,tumor necrosis factor (TNF)-α was showed to enhance the osteogenic differentiation of bone marrow derived mesenchymal stem cells (MSC). In this study,we aimed to investigate what effects of TNF-α pre-treated bone marrow MSC on myeloma cells when they were in vitro co-cultured. Methods:Normal bone marrow MSC was treated with TNF-α for once (T+1 cohort ) or once a day for seven days (T+7 cohort),then the TNF-α pre-treated MSC was directly co-cultured with myeloma cell line H929 cells for four days. At the end of co-culture,H929 cells were harvested and tested for the number of colony-forming units (CFU),mRNA levels of POU5F1,SOX2 and NANOG and miroRNA levels of miRNA-15a/16 by real-time PCR. The results from T+7 cohort were statistically compared with both T+1 and control cohorts. Results:TNF-α treatment did not cause any evidently morphological alterations on bone marrow MSCs. When compared with the control and T+1 cohort,our data showed the number of CFU in H929 was statistically decreased in T+7 cohort,the mRAN levels of POU5F1 and SOX2 were statistically down-regulated in T+7 cohort as well. However,the microRAN levels of both miRNA-15a and miRNA-16 were statistically up-regulated. Conclusion:Multiple TNF-α pre-treated bone marrow MSC was showed to has inhibitory effects on MM cells.

Abstract:Objective:To breed and identify of alkaline sphingomyelinase(Alkaline sphingomyelinase,alk-SMase)knockout mice. Methods:DNA was extracted from the mice tissue. PCR method was used for genotype identification. HE staining was used for morphology identification. Results:The gene kwockout mice has been successfully feed and breeded,and the generated alk-SMase knockout (KO) mice were gotten. Conclusion:The correct way to feed,breed and gene identification for gene knockout mice has important significance for obtainmont and transgenic mice preservation.

Abstract:Objective:To investigate the impacts of curcumin on mice with acute lung injury induced by sepsis and its mechanisms. Methods:Mice were divided randomly into CLP,sham,curcumin,captopril,and control groups. ALI/ARDS animal models were induced by the CLP operation. The acute lung injury of these animal was assessed by measuring oxygenation index,wet/dry lung weight rate (W/D),and lung tissue histology 18 h after operation. ELISA was applied to measured the level of TNF-α,IL-1β,and IL-6 in blood whereas radioimmunity was used to investigate the change of Ang II in lung tissue and blood. Results:Compared with the CLP group,W/D rate of lung tissue decreased whereas PaO2/FiO2 increased significantly in captopril and curcmin groups (W/D:5.35 ± 0.25,5.13 ± 0.59 vs 6.08 ± 0.64,P < 0.05);(PaO2/FiO2:259.5 ± 24.2,268.8 ± 21.5 vs 194.3 ± 23.9). Both captopril and curcmin markedly decreased Ang II level in lungs and plasma of mice(lung,1.58 ± 0.16,1.65 ± 0.21 vs 2.38 ± 0.41;blood,178.04 ± 17.87,153.74 ± 10.24 vs 213.38 ± 25.44). Several inflammatory factors such as TNF-α,IL-1β,and IL-6 decreased in serum and histopathologic appearance of ALI/ARDS attenuated after captopril or curcmin treatment. Conclusion:Curcmin could protect mice from acute lung injury induced by sepsis through inhabiting inflammatory factors release and Ang II generation.

Abstract:Objective:To observe the effect of recombinant human Granulocyte/macrophage colonystimulating factor(rhGM-CSF) and nano-silver as treatment for deep burn degreen Ⅱ wound healing. Methods:The burn models were done with Wistar rats, which were randomly divided into three groups,petrolatum treatment (group A. n = 30),nano-silver treatment (group B. n = 30),and rhGM-CSF treatment(group C. n = 30). The healing rate of the three groups was recorded on postburn day 1,4,7,10,14,21. Meanwhile the levels of VEGF and EGF in serums were measured with ELISA. Results:Group A,group B,and group C were all neovascularization and epithelization on postburn day 10. The healing rate:group C>group B>group A. On postburn day 10,there were no significantly difference between groups (P > 0.05),but on postburn day 14,21,there were significantly statistical differences between groups(all P < 0.05). For VEGF levels,group A,group B,and group C were peaked on postburn day 21,14,14,respectively. On postburn day 1,there were no significantly statistical difference between groups (P > 0.05).On postburn day 4,7,10,14 and 21,there were significantly statistical difference between groups (P < 0.05). For EGF levels,All groups were peaked on postburn day 21. On postburn day 1 and 4,there were no significantly statistical differences between groups (P > 0.05). On postburn day 7 there wrer differences between C and group A and group B,and on day 10,14,21 differences were observed between groups (P < 0.05). Conclusions: rhGM-CSF and nano-silver treatment could promote wound healing,but rhGM-CSF is better than nano-silver.

Abstract:Objective:To investigate the role and mechanism of phloroglucinol in hepatic ischemia reperfusion injury. Methods:Forty C57BL/6 mice were randomly divided into four groups on average,named as sham group,IR group,IR+Phlo group,and IR+PBS group. The sham group only separated portal vein without clipping,while partial liver ischemia reperfusion model (70%)was established in other three groups. RAW264.7,a mouse normal macrophage cell line,was incubated with or without phloroglucinol followed by stimulation of LPS in vitro. We evaluated the degree of liver injury by examining the levels of serum ALT and AST and histopathology graded by Suzuki’s score. Expressions of liver IL-1β and TNF-α mRNA were detected in real-time qPCR. ELISA was used to measure the levels of supernatant IL-1β and TNF-α of RAW264.7. The expression of phospho-p65 in RAW264.7 was assessed by western blot. Results:The degree of liver injury and the levels of liver IL-1β and TNF-α mRNA in IR+Phlo group decreased compared to those in IR and IR+PBS groups (P < 0.05). Preincubation with phloroglucinol could significantly inhibit the ablity of secretion of IL-1β and TNF-α by RAW264.7 (P < 0.05),which involved the supression of the activation of NF-κB. Conclusion:Phloroglucinol plays a protective role during hepatic ischemia reperfusion injury,which is partially relates to the supression of the activation of NF-κB in macrophage.

Abstract:Objective:A curative effect comparison on treatment of developmental cervical spinal Stenosis and cervical disc herniation by different procedures of posterior cervical Spine,which can provide theoretical support for two clinical operational choices. Methods:This paper reviewed 73 cases clinical data,which adopted two different posterior cervical spine procedures(single-door decompression laminoplasty or laminectomy lateral mass fixation)to treat patients with developmental cervical stenosis and cervical disc herniation,including 35 cases for single-door decompression laminoplasty and 38 cases for laminectomy lateral mass fixation. It also recorded the two group information like age,gender,surgical time,blood loss,morbid state,incidence of C5 nerve root palsy,JOA score,VAS score to compare the difference of two surgical procedures. Results:Compared with pre-operation,the postoperative symptoms and signs of both groups in this study improved obviously (P < 0.05). The significant difference was found in indicators of surgical time,blood loss,JOA scores after one-week surgery and its improvement rate,incidence of C5 nerve root palsy symptoms(P < 0.05),with the rest of indicators between two groups were not observed. Conclusion:Laminectomy lateral mass fixation can early ease patients’ neurologic symptoms who suffered from developmental cervical spinal stenosis and cervical disc herniation,and two kinds of operations were similar in last follow-up curative effect. However,single-door decompression laminoplasty owns advantages like shorter operation time,less intraoperative bleeding,incidence of C5 nerve root palsy symptoms.

Abstract:Objective:Although many studies have shown association of obesity and breast cancer,the association with the axillary lymph node status is not clear. We examined the relationship of the axillary lymph node status and obesity and other possible factors in breast cancer patients. Methods:In this retrospective cohort study,306 breast cancer patients were included. Patients were grouped according to their body mass index (BMI) values at the time of diagnosis. We analyzed the relationship between BMI and patient and tumor characteristics,especially axillary lymph node status. Results:The median patient age was 48 years(range 21~81). Median BMI of the patients was 25.1 and 54.4%(N = 166)of them had normal BMI,45.6%(n = 140)were BMI ≥ 25. Of the patients,59.6% had no lymph node metastasis,and 40.4% had lymph node metastasis. The total number of lymph nodes removed was higher in the obese group (BMI≥ 25)and this difference was statistically significant (20.4 ± 11.4 vs. 18.1 ± 10.5,P < 0.01). However,there was no statistically significant correlation between the number of metastatic lymph nodes and BMI(P > 0.05). Conclusion:The number of dissected lymph nodes was higher in obese patients but there was no correlation between metastatic lymph node number and BMI.

Abstract:Objective:To explore the effect of combined ARM and FNAC on postoperative lymphedema in ALND. Methods:This study was performed from Jan 2013 to Dec 2013 and patients undergoing ALND were enrdled. The patients were devided into control and treatment groups. One ml of Carbon Nanoparticles intradermally was injected in the ipsilateral upper extremity. FNAC was performed in both groups. All the dyed nodes were dissected in the control group. ARM nodes which were positive for malignancy,suspicious,or inadequate for diagnosis were dissected,while negative ARM nodes were spared. Data were collected with successful identification and protection of the arm lymphatics,and occurrence of lymphedema. Results:Of the 72 patients undergoing ALND,in 100% of patients,all ARM lymphatics and nodes were identified in the axilla. One,3,6 patients in control group and 2,2,9 with selective group had positive,suspicious and Unidentified ARM nodes by FNAC,respectively. Two in control group and 3 with the other had positive ARM nodes by histological diagnosis. Lymphedema in two weeks and postoperative 6 months happened in 88.6% and 31.4% of control,84.6% and 30.8% in the selective patients whose ARM nodes were removed,but 12.5% and none in the selective patients whose ARM nodes were preserved. other 8 cases whose blue lymphatics and nodes were preserved. Conclusion:ARM and FNAC can safely and effectively identify the upper extremity lymph drainage,so may be used to prevent the lymphedema after ALND.

Abstract:Objective:To investigate the expression and clinicopathologic significance of PHLPP1 and PHLPP2 in esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma (ESCC). Methods:qRT-PCR was used to quantify PHLPP1 and PHLPP2 mRNA expression in 40 pairs of esophageal intraepithelial neoplasia and their matched normal tissues,and 63 pairs of ESCC tissues and their matched normal esophageal tissues. The association of PHLPP1 and PHLPP2 expression with the clinicopathologic features was evaluated in 103 cases of esophageal intraepithelial neoplasia and ESCC patients. Results:The mRNA expression of PHLPP1 and PHLPP2 was significantly downregulated in ESCC tissue samples compared to the matched normal tissues and intraepithelial neoplasia tissues (P < 0.05). Levels of PHLPP1 or PHLPP2 mRNA had no significantly change in esophageal intraepithelial neoplasia compared to their matched normal tissues (P > 0.05). PHLPP1 and PHLPP2 were expressed at relatively high levels in ESCC with an early pathological grade (Ⅰ/Ⅱ),and markedly decreased in Grade III tumors (P < 0.01). In addition,the expression of PHLPP1 and PHLPP2 between patients with TNM stage Ⅰ/Ⅱ and stage Ⅲ of ESCC tissues was statistically significant (P < 0.01). Conclusion:Aberrant expression of PHLPP1 and PHLPP2 was closely related to the development and progression of ESCC. PHLPP1 and PHLPP2 may act as a prognostic marker for this widespread disease.

Abstract:Objective:To evaluate effects of muscle relaxant on treatment of severe asthma with mechanical ventilation by retrospective analysis. Methods:Forty-one patients with severe asthma treated by intubation and mechanical ventilation were divided into muscle relaxants group(muscle relaxants and traditional treatment)and non-muscle relaxants group(traditional treatment only). The blood gas analysis,airway pressure,extubation time,and hospitalization time were compared between two groups. Moreover,the extubation time and hospitalization time of the patients treated with muscle relaxants more than 48 hours or less than 48 h were analyzed. Results:Compared to the non-muscle relaxants group,the pH value and PaCO2 of arterial blood in muscle relaxants group were significantly improved(P < 0.05),the peak pressure of airway was significantly decreased(P < 0.05)2 h after muscle relaxants treatment. No difference was found in PaO2 between two groups. The hospitalization time was shorter in the non-muscle relaxants group(P < 0.05),while no difference was found in extubation time between two groups. Notably,when using muscle relaxants more than 48 h,both extubation time and hospitalization time were significantly increased. Conclusion:Muscle relaxants can rapidly improve the pH value,decreased the PaCO2 and peak pressure of airway in severe asthma patients after mechanical ventilation. However,they would increase extubation time and hospitalization time.

Abstract:Objective:To verify the existence of extraradicular biofilm of teeth with radiographically visible chronic periapical lesion and to observe the distribution and viability of extraradicular bacteria using confocal laser scanning microscopy(CLSM). Methods:Ten single-rooted teeth with periapical radiolucent areas were collected. Five single-rooted teeth extracted for orthodontic reasons were selected as controls. All of the roots were embedded in methylmethacrylate after staining with LIVE/DEAD■ BacLightTM Bacterial Viability Kits. Then,five evenly distributed transverse sections(1 mm thick)were cut from the apex to the corona. Finally,the sections were examined by CLSM. Results:There were weak auto-fluorescence signals in the control group. After adjusting the viewing conditions to obtain a baseline,at which no fluorescence was detectable in the control group specimens,8 of the 10 disease group teeth showed positive fluorescence signals on the external root surfaces. The distribution of extraradicular bacteria was extensive within a 2~3 mm range from the root tip to the corona. On 68% of optical views,the intensity of green fluorescence signals was stronger than that of red fluorescence signals. Conclusion:There were live and dead extraradicular bacteria on the external root surfaces of teeth with chronic periapical periodontitis.

Abstract:Objective:To evaluate the biocompatibility of two dental alloys (Au alloy and Ni-Cr alloy )and to investigate effects of two alloys expression of Bax and Bcl-2 of mouse fibroblast cells L929,try to find a new experimental method to evalute the biocompatibility of dental materials at molecular level. Methods: The fibroblast L929 cells were treated with leaching liquids of Au alloy (groupA) and Ni-Cr alloy (groupB),the RPMI 1640 cell medium containing 10% fetal calf serum was served as a negative control(groupC). The cytotoxicities of two alloys was evaluated by MTT and their effects on the expression of Bax and Bcl-2 were examined by immunohistochemistry and ELISA method. Results: The cytotoxicity of two alloys was in Grade 0,the ratio of Bax/Bcl-2 was higher in Ni-Cr alloy group than that of other groups,and the differents were significant. Conclusion:The results suggested that the leaching liquids of Ni-Cr alloy may induce Bax protein expression and Bax/Bcl-2 ratio increase;the results may provide new ideas for the future evaluation of biotic materials for compatibility at the molecular leval.

Abstract:Objective:To investigate the effect of liquiritigenin (LQ) on cell migration and invasion by monitoring regulation of miRNA and its downstream target gene expression in human melanoma A375 cells. Methods:MTT assay was used to detect the cell viability of A375 cells treated with different concentrations of LQ. The effect of LQ on cells migration and invasion was measured by wound healing method and Transwell migration/invasion assay. Decreased expression of hsa-miR-4487 and hsa-miR-4534 was screened by miRNA Expression Array,and confirmed by quantitative PCR. The expression levels of PTEN,p-AKT,AKT,MMP2,and TIMP2 were examined by Western blot. Results:Ten to 100 μmol/L of LQ treatment on A375 cells for 24 h had no obvious damage to cells. LQ inhibited cell migration and invasion in a dose-dependent manner. Hsa-miR-4534 and hsa-miR-4487 expression were downregulated by LQ. Meanwhile,LQ increased the expression level of PTEN and TIMP2,and decreased p-AKT and MMP2 expression in A375 cells. Conclusion:LQ inhibits migration and invasion of A375 cells,probably via regulating miRNA expression and its downstream target genes.

Abstract:Objective:To analyze laboratory diagnosis coincidence rate of hepatitis B in Jiangsu Province in 2012. Methods:Stratified random method was used to chose 2-3 cities from each of south,middle and north of Jiangsu Province,and further chose the provincial,municipal and county hospitals from each city. Results:The laboratory diagnosis coincidence rate was 75.26% based on effective 287 cases from a total of 23 county-level or above medical institutions. The diagnostic accordance rate in the provincial hospitals was the lowest. The awareness rates of laboratory diagnostic criteria and report type of HBV for clinicians were 23.38% and 36.52%,respectively,the highest of which was from country hospitals. Conclusion:The laboratory diagnosis coincidence rate of hepatitis B in Jiangsu Province is relative high,however,the awareness rate about HBV relevant knowledge needs to be improved. Therefore,it is necessary to strengthen the training and communication,improve the HBV monitoring network as well as propel practical HBV diagnostic criteria.

Abstract:Objective:To research the application of State Space Model forecasting in infectious disease incidence,and discuss the method to improve its veracity and practicability. Methods:A model was fitted by the historical data of the incidence of tuberculosis in China. Firstly,used the ratio to moving average method and HP filter to make preanalysis. Secondly,parameters of model is estimated and a State Space Model was set up by decison of the rank of it. Finally,the paper tests the result of forecast and analysis it. Results:State Space Model can decompose some characteristic components from the changing process of the incidence. Forecast accuracy of the Monthly incidence in a year is above 90%. Conclusion:The fit values of incidence are consistent with the actual data of incidence and the forecasting effect is good.

Abstract:Objective:To investigate the situation of injury comprehensive surveillance system,find and solve the problem,provide the information base for the work of injury prevention and control. Methods:The data from 2011 to 2012 was used to analyze epidemic situation of injury,characteristics of the hospitalization expense and injury composition,and describe the surveillance work and existing problems. Results:The analysis of injury mortality showed that the resident population number of two surveillance areas was 1 971 633 in 2011 year,the injury mortality was 50.46∕100 000,and 1 985 232 in 2012 year,the injury mortality was 51.28/100 000;The male injury mortality was higher than women in both two surveillance areas from 2011 to 2012;The total Hospitalization expense of two surveillance areas was 208 million,the average expense was 10 751.02 yuan. Hospitalization expense caused by Accidental falls was the highest both in two surveillance areas,the second was Road Accidents,and the expenses were 19 803.42 yuan and 17 061.04 yuan in Pukou district,1 3387.77 yuan and 12 728.26 yuan in Zhangjiagang city;The comparisons of basic characteristics were all statistically significant in two surveillance areas. The top two reasons of Death,Hospitalization,Outpatient and emergency were still Road Accidents and Accidental falls. Conclusion: The hospitalization expense caused by Road Accidents and Accidental falls were the main injury causes,and were also the main reason leading to death,hospitalization,outpatient and emergency,so we should strengthen the integrated control of the two injures,reduce losses caused by the injuregs. There were some problems in the injury comprehensive surveillance system with low information utilization,and we should improve the quality of surveillance data.

Abstract:Objective:To monitor the Salmonella contamination status of commercial chicken products in Nanjing city through detecting Salmonella qualitatively and quantitatively. Methods:Retail chicken products were collected from supermarkets and farmers markets in different region of Nanjing city. Samples were examined by polymerase chain reaction (PCR) and most probable number (MPN) method. Cumulative distribution was used to fit the contamination level of the positive and negative samples,and Discrete distribution was used to all. Results:Of the total 288 chicken products,43 (14.9%) tested positive for Salmonella. In regard to the positive rate of Salmonella,no significant difference was observed among the six different samples. Prevalence of Salmonella in chicken products from farmers markets was higher than that from supermarkets. During the eight surveillance monthes,the top two contamination rate was detected in June and July,27.5% and 20.0% respectively . MPN value of negative samples was 0.009 MPN perg and that of positive samples was 1.080 MPN per g,0.018 MPN per g for the average. Conclusion:The overall pollution situation of Salmonella in retail raw chicken products of Nanjing had aroused public concern,enhanced quantitative monitoring of the Salmonella infection was needed.

Abstract:Objective:To investigate effects of microRNA-145 (miRNA-145) on growth and angiogenesis of osteosarcoma in vivo. Methods: Sixteen nude mice (Balb/c)were randomly divided into 2 groups,experimental group and control group,with 8 mice in each group. The animal model of experimental group was constructed by subcutaneous inoculation of osteosarcoma cells transfected with miRNA-145,and control group using subcutaneous inoculation of osteosarcoma cells. The osteosarcoma-bearing nude mice were feeded for 4 weeks. After 4 weeks,tumor formation rate,size,and weight of tumor were observed in each group. Ⅷ factor expression and VEGF expression of tumor were detected by immunohistochemistry and microvessel density were calculated. Results: The tumor formation rate is 87.5% and 37.5% in control group and experimental group respectively. The size and weight of tumor in experimental group is significantly less than that of the control group (P < 0.05). The VEGF expression and MVD of tumor in the experimental group is significantly lower than those in that of the control group (P < 0.05). Conclusion: miRNA-145 can inhibit osteosarcoma formation of nude mice,and inhibit growth and angiopoiesis of tumor in vivo.

Abstract:Objective:To observe the effects and mechanisms of beraprost on cross linked collagen in angiotensinⅡ (AngⅡ)-stimulated cardiac fibroblasts. Methods:The cultured neonatal rat cardiac fibroblasts were pre-treated with beraprost followed with Ang Ⅱ (100 nmol/L) stimulation for 24 h. Total collagen and cross linked collagen were examined in culture medium. Lysyl oxidase (LOX) expression was determined with real time PCR and western blot. Results:Beraprost dose-dependently inhibited collagen secretion with Ang Ⅱ stimulation,among which 10 μmol/L presented with the most robust suppression. Beraprost (10 μmol/L) pre-treatment for 4 h followed with AngⅡ stimulation significantly reduced collagen secretion,as well as the cross linked degree and ratio of cross linked collagen to non-cross linked collagen,accompaying with decreasing mRNA and protein expression of LOX. Conclusion:Beraprost inhibited cross linked collagen in AngⅡ-stimulated cardiac fibroblasts,which may be associated with suppression of LOX expression.