Abstract:Objective:This paper focus on the correlation and clinical pathological significance of toll-like receptor 3(TLR3) and its signal molecules TIR-domain-containing adaptor protein including IFN-β (TRIF) and autophagy related gene,LC3 and Beclin 1,expressions in human hepatocellular carcinoma (HCC),and its relevant mechanism. Methods:We collected and followed-up 101 cases of HCC cases,and built tissue microarray. HE staining for HCC tissue differentiation and immunohistochemical staining were performed to detect the expression of Ki-67 (cell proliferation marker),TLR3,TRIF,LC3 and Beclin 1,respectively. TUNEL was performed to detect the apoptotic signals of cancer cells. Statistical analysis was performed to analyze its correlation with cancer clinical pathological factors and prognosis. Results:In HCC,the expressions of TLR3 and TRIF were positive correlated(ρ=0.640,P < 0.001). Its high expression was positively correlated with HbsAg infection,cirrhosis background,cell apoptosis index(AI) and total 5-year survival rate,and negatively correlated with cancer cell proliferation,vascular invasion,Edmondson grading and TNM staging. Beclin1 and LC3 expression was significantly positive correlated(ρ=0.587,P < 0.001). Both high expressions were respectively positively correlated with vascular infiltration,Edmondson grading,TNM staging and cancer cell proliferation,but negatively correlated with apoptosis index(AI) and 5-year survival rate(P < 0.001). TLR3 and TRIF respectively negatively correlated with the expression of LC3 and Beclin 1(both P < 0.001). Conclusion:The expressions of TLR3 and signaling molecules TRIF,cell autophagy related gene Beclin1 and LC3 respectively influence the biological behavior and prognosis of HCC by regulating cell proliferation,apoptosis and autophagy. There are cross points existing between Beclin1 mediated autophagy pathway and TLR3 mediated apoptosis pathway in HCC. Abnormalities of the two pathways may be one of the mechanisms in HCC occurrence and progress.

Abstract:Objective:Zinc-alpha-2-glycoprotein 1(ZAG) is a protein with multiple functions,such as participating in lipometabolism,saccharometabolism,energy metabolism,immunoreaction and fertilization. It is observed whether ZAG involved in the regulation of human colorectal cancer cell line(HT-29) in this study. Methods:ZAG-RNAi plasmids were constructed and transfected into human colorectal cancer HT-29 cells. The expressions of target proteins and gene were detected by Western blot and RT-PCR,respectively. Adenosine triphosphate (ATP) was assessed to investigate the activation of energy metabolism. The capacity of HT-29 cell migration was investigated using the Transwell migration assay. Results:ZAG interference plasmids were successfully transfected into HT-29 cells,and stably transfected cell lines were screened out. ATP level and the migration of ZAG inhibitor-treated cells were increased. Conclusion:The down-expression of ZAG promoted the expression of ATP and up-regulated the malignant phenotype of HT-29 cells.

Abstract:Objective:To study the effecs of human trophoblast cell surface glycoprotein(Trop2) overexpression on migration of the human gastric mucosal epithelial cells and preliminary mechanism analyses. Methods:We explored mRNA and protein expression levels of Trop2 in GES-1 cell line by real-time RT-PCR and Western blot. OE-Trop2 and VE-Trop2 plasmids were constructed and then transfected into GES-1 cell line,and the efficiency of transfection was detected. Cell wound healing assay and Transwell migration assay were performed to detect the change of the migration in GES-1 after transfected OE-Trop2 plasmids. The change of surface markers(E-cadherin,fibronectin,and vimentin)of epithelial-mesenchymal transition(EMT)in GES-1 cell line were detected by Western blot after Trop2 overexpression. Results:Compared with gastric cancer cell lines BGC823 and MGC803,the mRNA and protein expression levels of Trop2 in GES-1 were lower. After OE/VE-Trop2 plasmid transfected in GES-1,the mRNA expression level of Trop2 in the OE-Trop2 group was 6.18 ± 0.52 times higher than that of the control group,whereas no differences were detected between the control group and the VE-Trop2 group. Meanwhile,the protein expression model of Trop2 was similar to that of the mRNA expression. After transfected plasmids 72 h,cell wound healing assay showed that the migration rate of GES-1 was 50% in the VE-Trop2 group and 95% in the OE-Trop2 group(P < 0.05). After transfected OE-Trop2 plasmids 24 h,transwell migration assay showed that the number of migration cell was 41 ± 6 in the VE-Trop2 group,whereas 87 ± 6 in the OE-Trop2 group(P < 0.05). Western-blot assay showed that the expression level of E-cadherin in GES-1 was down-regulated;meanwhile,fibronectin and vimentin were up-regulated after transfected OE-Trop2. Conclusion:Trop2 overexpression could promote migration of GES-1 cell line through inducing EMT phenomenon.

Abstract:Objective:By using carboxylated agar magnetic beads as selection medium,we sought to use RT-PCR and target replacement subtractive SELEX technology from the serum of gastric cancer screening to obtain aptamers with high specificity and affinity. Methods:The target molecules were gastric cancer serum performed with centrifugal ultrafiltration(ultrafiltration 50 000),and the carriers were the carboxyled agar magnetic beads. Firstly,oligonucleotide library and anti-screening agar magnetic beads(normal serum binding)were bound,then supernatant was combined with screening magnetic beads (gastric binding serum). We washed away unbound oligonucleotide molecules,and oligonucleotide molecules binding on the magnetic beads were isolated and amplified. λ enzyme digestion method was performed to prepare ssDNA second library for 10 rounds of screening. The library of the 10th round was amplified to obtain dsDNA,then we used gel extraction kit recovering purified fragment of dsDNA and connected it with pMDTM18-T vector and transformed into E. coli DH5α competent cells. we selected positive cloning group and sequenced them to obtain aptamers sequence. Simultaneous determination of Kd values of gastric cancer serum aptamer was performed by flow cytometry. Results:After 10 rounds of selection,20 gastric cancer serum specific aptamers were screened. Conclusion:The specific detection showed that the Kd values of gastric cancer serum binding aptamers were at the nanomolar level,and No.5,7,16,17,18 aptamers can bind to gastric cancer serum with high specificity and affinity,and didn’t bind to normal human serum. It provides an experimental basis for the early diagnosis of gastric cancer.

Abstract:Objective: To separate and extract effective components from Viscum coloratum(Kom.) Nakai and investigate their effects on the proliferation of breast cancer cells. Methods: The classical separation and extraction methods were used firstly,and then according to different solubilities of alkaloids from Viscum coloratum(Kom.) Nakai in various solvents with different pHs,different components were extracted and obtained. The components were dissolved and cultured with breast cancer cells in vitro. Results: About 10 components were obtained and different inhibition effects were observed in co-culture with breast cancer cells. Component 5 inhibited the proliferation of breast cancer cells significantly in vitro and the IC50 for breast cancer cell line MDA-MB-435 and MDA-MB-231 was 3.24 mg/mL and 4.80 -滋g/ml,respectively. Conclusion:In vitro,series of extracts from Viscum coloratum(Kom) Nakai inhibited on breast cancer cells proliferation,and component 5 had significant inhibition effects on the cancer cells.

Abstract:Objective:To investigate the activity and acetylation of FoxO1 after inflammatory cytokine IFN-γ treatment in mouse myoblast cells(C2C12) and its regulatory mechanisms on T2DM. Methods:Acetylation of FoxO1 after IFN-γ and silent information regulator 1 (SIRT1) activator resveratrol treatment were evaluated by co-immunoprecipitation (co-IP) followed by Western blot. Luciferase reporter assays were performed to assess the effect of IFN-γ and C Ⅱ TA on the promoter activities of FoxO1. To evaluate whether CⅡTA was necessary for the repression of SIRT1 activity by IFN-γ,cells were transfected with C Ⅱ TA small interfering RNA(siRNA). Results:IFN-γ augmented the acetylation level of FoxO1 while down-regulated by resveratrol. IFN-γ suppressed the transcriptional activity of wild-type FoxO1,mutation of lysine residues in FoxO1 abrogated such effects. C Ⅱ TA directly inhibited FoxO1 dependent transcription and while transfected with FoxO1 simultaneously,the inhibition was up to 60%. C Ⅱ TA depletion restored FoxO1 transcriptional activity in the presence of IFN-γ. Conclusion:IFN-γ induced the expression of C Ⅱ TA,which in turn inhibited SIRT1 enzyme activity,increased the acetylation level of FoxO1,thus reduced its transcriptional activity. Therefore,our study provides a potential target for clinical intervention in T2DM.

Abstract:Objective:To explore the effects of MnTBAP on puromycin aminonucleoside (PAN)-induced nephrotic rats. Methods:A total of 24 male Sprague-Dawley(SD)rats were randomly divided into the following groups(n=8 for each group):the control group,the PAN group(one single dose-150 mg/kg of PAN was injected into rats by tail-vein injection)and the MnTBAP-treated group[on the day with injection of PAN,the rats were treated with a daily intraperitoneal injection of 10 mg/(kg·d) MnTBAP for 14 days]. Twenty-four hours urinary protein excretion was detected by the Bradford protein assay. The ultrastructure of glomerular potocyte was observed by the transmission electron microscope. Nephrin and podocin expression levels were measured by real-time PCR and Western blot. Results:①24 hours urinary protein excretion and the renal index of PAN group were significantly increased compared to those of the control group(P < 0.01),while the 24 h urinary protein and renal index of the MnTBAP-treated group was lower than those of the PAN group (P < 0.01). ②The transmission electron microscope results showed that glomerular podocyte in the PAN group fused and disappeared widely,but it markedly alleviated after MnTBAP treatment. ③Real-time PCR showed that the expression of nephrin and podocin in the PAN group were reduced in comparison to those of the control group(P < 0.01),which was restored by MnTBAP treatment(P < 0.01);④Western blot showed that the expressions of nephrin and podocin in the PAN group were reduced in comparison to those of the control group(P < 0.01),which was restored by MnTBAP treatment(P < 0.01). Conclusion:MnTBAP could decrease urinary protein and podocyte injury in PAN-induced nephrotic rats.

Abstract:Objective:To investigate the protective roles of lipoxin A4 (LXA4) in hyperoxia-induced bronchopulmonary dysplasia(BPD) in neonatal mice and the possible mechanisms. Methods:BPD was induced by 85% O2 exposure. C57/BL6 neonatal mice were randomized divided into the air group,the hyperoxia(85% oxygen)group,the hyperoxia +LXA4 group,and the hyperoxia + TGF-β1 antibodies (1D11) group. The weight and growth status of the mice were recorded daily. The pathologic changes of pulmonary tissues were observed by hemotoxylin and eosin (HE) staining. Not only the expression of fibrosis indexes of the lung,but also quantitative expressions of TGF-β1 receptor(TGF-βR1)and TGF-βR2 were detected by real-time quantitative PCR. Results:①Model neonatal mice under hyperoxia circumstance presented growth retardation and poor activity compared with the air controls.②Compared with the air group,decrease of volume and darkness of color were observed in pulmonary tissues of mice in the other three groups on d7. However,the colors of pulmonary tissues in the LXA4 and the 1D11 treated groups were similar with the air group on d13. ③Compared with the air group on d7,the pathology changes of other three groups were consistent with BPD. In comparison to the models on d13,treatment with LXA4 or 1D11 led to the normalization of alveolar structure.④Hyperoxia resulted in significant increase in the mRNA expressions of fibrosis indexes in the lung,such as,fibronectin,α-SMA,elastin,tenascin-C and TIMP-1. There were also overexpression of TGF-βR1 and TGF-βR2(P < 0.05). The uptrend of expression of MMP-1 was found in both the LXA4 group and the 1D11 group,which was opposite in the hyperoxia group. Furthermore,it was more notable in the mice treated with LXA4. The quantity of collagen I expression was promoted by hyperoxia exposure(P < 0.05) compared with controls. The reduction of collagen I was detected both in treatment of LXA4 and 1D11 (P < 0.05). Conclusion:LXA4 can play a protective role in hyperoxia-induced BPD in neonatal mice by reducing the expression of fibrosis indexes modulated by the signaling pathways of TGF-β1.

Abstract:Objective:To investigate the possible deficiencies in the transcription,secretion,and expression of regulatory T cells(Treg) associated TGF-β in patients with systemic lupus erythematosus(SLE). Methods:Peripheral blood mononuclear cells(PBMC)and CD4+ CD25+ T cells were isolated from patients with SLE and health controls. The cells were cultured and stimulated with anti-CD3 and anti-CD28 for 48 hours. Three-color flow cytometry was performed to detect CD4+CD25+LAP+T and CD8+CD25+LAP+T cells; Q-RT-PCR was used to detect TGF-β mRNA level; ELISA was performed to detect the levels of total TGF-β1 and free active TGF-β1 in the supernatants of the cell cultures. Results:The ratios of LAP+/CD4+ T,CD25+ LAP+/CD4+ T and LAP+/CD4+CD25+ in fresh PBMCs,or in cultured cells stimulated with anti-CD3 and anti-CD28,from active SLE patients were both significantly higher than those from health controls (P < 0.05),but there were no significantly correlations between the abnormally increased LAP expression and SLE disease activity index (SLEDAI). Whether stimulated or not,mean fluorescent intensity (MFI) of LAP in CD4+CD25+LAP+ cells from patients with SLE was also higher than that from health controls(P < 0.05). With the stimulation,CD4+CD25+ T cells from the patients secreted less TGF-β1,both in the forms of total TGF-β1 and free active TGF-β1 (P < 0.05). Furthermore,CD4+CD25+ T cells from active SLE patients displayed less TGF-β mRNA level than those from health controls did. Conclusion:In CD4+CD25+ T cells from SLE,there are deficiencies in the transcription and secretion of TGF-β1,and in the production of free active TGF-β1,although the expression of LAP on the cell surface is increased,which may reflect the abnormal activation of T cells and a proliferative respond of CD4+CD25+ LAP+ Treg to auto-antigens. These deficiencies in TGF-β expression of CD4+CD25+T cells in active SLE patients probably weaken the function of Treg cells.

Abstract:Objective:To screen and obtain hybridoma cells secreting antibody of group A Neisseria meningitidis polysaccharide,produce monoclonal antibody,and establish an indirect compete ELISA based on McAb to detect A polysaccharide content in group ACYW135 meningococcal polysaccharide vaccine. Methods:Hybridoma cells were screened by hybridoma technology,McAb was produced by injecting cells into mice abdominal cavity,the indirect compete ELISA was developed through optimizing the test conditions,A polysaccharide content was detected in 5 groups of ACYW135 meningococcal polysaccharide vaccines from the same batch and 3 from different patches separately. Data were analyzed through one-samples T test in SPSS. Results:The equation of linear regression after Log-logit processing:y=-1.664 8-2.254 2x,R2 =0.989 5,the linear detection range was 0.022 8~1.200 7 μg/mL,IC50=0.165 8 μg/mL,and the lowest detection limit of this method was 0.022 8 μg/mL. A polysaccharide content was detected in the vaccines. There were no significant differences between detected value and original value(P > 0.05). Conclusion:We successfully screened the hybridoma cell and produced the McAb,provided indirect compete ELISA with good sensitivity,stability and repeatability,which detects A polysaccharide content in the process of meningococcal vaccine. It can overcome the difficulties that phosphorus content method and rocket eletroimmunoassay have.

Abstract:Objective:To describe the distribution of polymorphic site of the new short tandem repeat (STR) loci in Jiangsu Chinese Han population. Methods:A total of 9 STR loci were amplified by STRtyper-10G Direct Kit and genotyped by Genetic Analyzer,and all samples were collected from 598 unrelated individuals in Jiangsu Han population during 2015. Results:A total of 111 genotypes were found,of which 13 were rare alleles. The frequency,greater than 0.08,was 13,of which the maximum frequency of “19/20” from D13S325 locus was 0.130 4. Heterozygosity was 0.851 ± 0.068,matching probability was 0.054 ± 0.022,polymorphism information content was 0.808 ± 0.052,power of discrimination was 0.946 ± 0.022 and probability of paternity exclusion was 0.697 ± 0.130. Conclusion:With the analysis of alleles in a large number of samples and frequencies,objective alleles of more genetic polymorphism data of Jiangsu population should be obtained,so that the need of further practical application could be met in the case of micro alterations.

Abstract:Objective:To analyze polymorphism distribution of 12 non-CODIS STR(including D11S4463,D2S1776,D14S1434,D4S2408,D10S1043,D20S482,D12SATA63,D19S433,etc.)of Jiangsu population by 3130 genetic gene sequencing electrophoretograms,and to calculate the gene frequency(P),discrimination power(DP),heterozygosity(H),polymorphic information content(PIC),probability of exclusion of due-testing(PEduo)and probability of exclusion of trios-testing (PEtrio). And then,to evaluate its forensic application value. Methods:The products of PCR amplification mere detected by capillary electrophoresis usiny 3130 sequenator. Data were analyzed by analysis software. Results:The frequency distributions of the 12 non-STR CODIS loci were consistent with the Hardy-Weinberg equilibrium. H ranged from 0.707 6 to 0.804 6,DP was 0.846 8 to 0.948 6,PEduo was 0.278 6 to 0.482 6,PEtrio was 0.446 7 to 0.663 2,and PIC was 0.643 8 to 0.807 4. Conclusion:Through the statistics of a large amount of experimental samples and allele frequencies,the allelic polymorphism data were obtained for comparison and verification. It has a good auxiliary function and play an important complementary role in cases of CPI less than 10 000 and mutation.

Abstract:Objective:To investigate the factors affect on fractional flow reserve(FFR),and to evaluate the value of FFR in guiding stenting. Methods:Sixty patients with coronary heart disease and hospitalized in the department of cardiology of the First People’s Hospital of Zhangjiagang City from February 2012 to January 2015 were retrospectivly enrolled,including patients with chest tightness and chest pains,suffered acute myocardial infarction and those who underwent coronary angiography again after stenting. General data such as age,gender,smoking history and the levels of low-density lipoprotein-cholestero l(LDL-C),total cholesterol(TC),triglyceride(TG)and blood glucose(BG)were recorded. All patients underwent coronary angiography and FFR measurement. The degree of stenosis and length were recorded. Patients were divided into two groups:FFR≥0.8 and FFR<0.8. General data were compared between the two groups,and the correlations with FFR were analyzed. Stents were implanted only when the FFR<0.80,and FFR were measured again when stenting were finished. We compared FFR before and after stenting. The numbers of readmission for cardiac causes,a new episode of angina,revascularization of the target lesion,arrhythmia,heart failure and acute myocardial infarction were recorded and compared between the two groups in the first year. Results:①All of the 60 patients had completed coronary angiography and FFR measurement. There were no differences of general data between the FFR≥0.8 group and the FFR<0.8 group. There were significant differences of degree of stenosis and the length of stenosis between the two groups(both P < 0.001),and there were significant negative correlations between FFR and degree of stenosis and the length of stenosis(P < 0.001).②In patients with FFR<0.8,there were significant differences in FFR before and after stenting(P < 0.001). There was no difference of FFR between patients after stenting in the FFR<0.8 group and those in the FFR≥0.8 group(P=0.085).③The numbers of LAD,RCA and LCX belonged to different degree of stenosis were calculated,and there were no differences of FFR in the same degree of stenosis in different coronary artery(P > 0.05). ④During one-year follow-up,there was no difference of a new episode of angina,revascularization of the target lesion,arrhythmia and heart failure between two groups(P > 0.05). There was no acute myocardial infarctions event at 1 year in both groups. Conclusion:There were significant negative correlations between FFR and the degree of stenosis and the length of stenosis. This indicates that the more severe of stenosis and the longer of stenosis length,the more significant influence on the functional repercussion of coronary. FFR measurement is useful in guiding PCI treatment and evaluating the value of stenting.

Abstract:Objective:To explore the diagnostic significance of pentraxin 3(PTX3) in patients with Sjogren’s syndrome(SS). Methods:Plasma from 52 patients with SS and 15 healthy volunteers was detected for the level of PTX3 by ELISA. Correlation between PTX3 and clinical indexes of the patients with SS was analyzed. Results:Level of PTX3 in SS patients especially with tubular damage patients[(1.492 ± 0.018)ng/mL]was significantly higher than that in the control group[(1.403 ± 0.019)ng/mL](P < 0.01),and the plasma level of PTX3 [(1.573 ± 0.019)ng/mL]of patients(n=31)with renal tubular dysfunction(including abnormal urine acidification function,urine NAG and abnormal urinary β2-microglobulin)was significantly higher than that in patients without tubular damage[(1.418 ± 0.003)ng/mL](P < 0.01). Plasma PTX3 was negatively correlated with TA and NH4+ in the urine of SS with tubular damage(P < 0.05). However,there was no correlation between ESR,CRP,IgG,IgA,IgM,SSA,SSB,and RF. Efficacy of PTX3 in the diagnosis of renal tubular dysfunction in patients with SS was equivalent to that of urinary β2-microglobulin. The area under ROC curve of PTX3 was 0.702(95%CI:0.593~0.811),which had no difference compared with urinary β2-microglobulin[AUC:0.705,95%CI:0.567~0.843]. Conclusion:Plasma PTX3 could be a useful marker of tubular damage in SS patients.

Abstract:Objective:This research aimed to explore the relationship between human serum ciliary neurotrophic factor and type 2 diabetes mellitus. Methods:A total of 101 patients of type 2 diabetes mellitus were separated into three groups,including DM group(n=37),DR group(n=34),and DPN group(n=30). Twenty cases of the health examination were enrolled the control group. General information was gathered including age,gender,diabetic course,height,weight and waistline. Biochemistry,C-peptide and glycosylated hemoglobin were detected by clinical laboratory and serum CNTF level was detected by ELISA. Results:①There were no significant differences in gender,age,height,weight,body mass index and waistline between groups. Diabetic course,fasting blood glucose,glycosylated hemoglobin(HbA1c),low-density lipoprotein cholesterol(LDL-C) in the group of DR and DPN were higher than those in the DM group. The level of estimated glomerular filtration rate in the DR group was(86.03 ± 20.80) mL/min,which was remarkable lower compared to the DM and DPN group. Compared with the normal group,serum CNTF in three groups with diabetes mellitus was distinctly reduced(F=49.612,P < 0.001). ②Diabetes mellitus complicated with retinopathy and peripheral neuropathy was negatively correlated with serum CNTF by Pearson correlation analysis(r=-0.741,P < 0.001). ③The result of Logistic regression analysis showed that CNTF,HbA1C and LDL-C were independent risk factors for the development of DR and DPN. Conclusion:The level of serum CNTF was significant lower in diabetic patients,and it was negatively correlated with diabetic complications. CNTF is one of the independent risk factors for diabetic retinopathy and peripheral neuropathy.

Abstract:Objective:To investigate the factors influencing the prognosis of patients with acute pulmonary embolism. Methods:The data of 245 patients with acute pulmonary embolism were analyzed retrospectively and the follow-up-visits were completed. The patients were divided into three groups including the acute phase death group,the late death group and the good prognosis group. The clinical data and the reasons of death were compared. Results:There were no significant differences in age,sex,smoking history,hypertension,diabetes,coronary heart disease,rheumatic heart disease,chronic kidney disease,connective tissue disease,pregnancy and cerebrovascular disease in the 3 groups. However,the ratios of malignant tumor and chronic heart failure in the two death groups were significantly higher than that in the good prognosis group,and the ratio of infectious diseases in the acute phase death group was significantly higher than that in the other two groups. Besides,the ratio of chronic lung disease in the late death group was significantly higher than that in the other two groups,and the ratio of surgical history in the good prognosis group was significantly higher than that in the other two groups. The progress of malignant tumor was the main reason of death in all patients. Moreover,there was a considerable proportion of patients died of chronic thromboembolic pulmonary artery hypertension and gastrointestinal bleeding in the late death group. Conclusion:After standard treatment,some of the risky factors of thrombosis did not significantly affect the prognosis of patients. On the other hand,malignant tumors,chronic lung disease,chronic heart failure and serious infectious disease could indicate bad prognosis of acute pulmonary embolism. The risk-stratification based on shock and simplified pulmonary embolism severity index score is an effective method to evaluate the prognosis of patients. We could focus on the occurrence of chronic thromboembolic pulmonary arterial hypertension and digestive tract hemorrhage in the long term follow-up-visits.

Abstract:Objective:To systematically review the clinical outcomes of drug-eluting stents (DES) guided by intravascular ultrasound(IVUS) versus coronary angiography (CAG) for the patients with coronary artery disease (CAD). Methods:We searched relevant literatures in PubMed,Embase,Cochrane Library,Scopus and Chinese Biomedical Database from January in 2000 to July in 2016,meanwhile,collected published data and studies from cardiovascular congresses to compare IVUS- and CAG-guided DES implantation for CAD patients. Meta-analyses were conducted by STATA 12.0 software. Results:Twenty-three eligible studies with a total of 31 685 patients were enrolled,of whom 3 192 patients were from randomized controlled trials. In general,intervention with DES guided by IVUS was associated with significant improvements on death(OR:0.63,95% CI:0.55~0.73,P < 0.001),myocardial infarction(MI,OR:0.69,95%CI:0.58~0.82,P < 0.001),major adverse cardiac events(MACE,OR:0.75,95%CI:0.69~0.82,P < 0.001),stent thrombosis(OR:0.56,95%CI:0.43~0.73,P < 0.001),target vessel revascularization(TVR,OR:0.79,95%CI:0.68~0.93,P < 0.001),and target lesion revascularization(TLR,OR:0.75,95%CI:0.62~0.91,P < 0.001),when compared with CAG guidance. Conclusion:IVUS guidance was associated with significantly improved clinical outcomes,including death,MI,MACE,stent thrombosis and re-intervention,compared with CAG guidance in the treatment of patients with CAD. Appropriately powered randomized trials are warranted to verify the findings of this meta-analysis and determine which types of patients and lesions will be benefit more from IVUS guidance.