Abstract:The main research achievements of functional mechanism,efficacy and safety of targeted drugs in metastatic colorectal cancer were reviewed in this paper for looking forward to the future development direction. Bevacizumab has remarkable curative effect in anti-angiogenesis drugs; curative effect of small molecule drug is worth expecting. Indications of drugs on the resistance to EGFR signaling pathway will be more accurate; the research of molecular markers such as BRAF,HER2 and PI3KCA further promote the development of the precision medicine. The researches on PD-1/PD-L1 in immune targeted drugs made a breakthrough in metastatic colorectal cancer. In the future under the guidance of molecular markers,precision medicine in metastatic colorectal cancer is more worth expecting than before.

Abstract:The multidisciplinary approach to colorectal cancer has been well accepted,recently. Meanwhile,individualized drug therapy has been developed by the guidance of molecular subtyping. Colorectal cancer is a heterogeneous disease that develops as a consequence of both genetic and epigenetic events. In the new era of big data and precision medicine,the molecular subtype of colorectal cancer is becoming more and more important,including differentiation of molecular biomarkers and locations of cancer. The future direction of colorectal cancer treatment should focus on dissecting tumor heterogeneity,distinguishing individual biological behavior,and practicing precision medicine.

Abstract:Neoadjuvant chemotherapy has become the standard treatment for locally advanced rectal cancer. The focus of the research is to predict the efficacy of neoadjuvant therapy,to enrich the advantages of people and avoid the excessive treatment as well as reduce unnecessary medical expenses. In the past several years,with the rapid development of gene sequencing technology,based on the gene expression of colorectal cancer molecular subtype to predict patient’s drug sensitivity and prognosis,individual patients with different gene type undergoing precise treatment has become the focus of the current treatment.

Abstract:Colorectal cancer is one of the commonest cancers in China and surgical treatment remains the major cure procedure. The precision, function preserving and micro surgery are the directions of surgical developing for colorectal cancer. Precision evaluation of colorectal cancer were discussed in this review.

Abstract:Bevacizumab (BEV),as the first antiangiogenic targeted drug approved by FDA in 2004,has been applied to first-line treatment of metastatic colorectal cancer (mCRC) wildly at present. However,no predictive marker has been identified yet for the efficacy and prognosis of BEV. To solve the clinical problem and determine the individuals who will acquire more benefits from BEV and achieve precise treatment,scholars have carried out many explorations in this field. A literature review was conducted to summarize the research progress and prospect about the predictive factors of BEV in the past years.

Abstract:Objective:We sought to construct a full human anti-Trop-2 IgG expression vector,and to express and purify it,then analyze the effect of anti-Trop-2 IgG in human ovarian cancer cells. Methods:The recombinant expression vector of full human anti-Trop-2 IgG was constructed and the IgG was expressed in 293 FreeStyle (293F)cell eukaryotic expression system and then purified using AKTA system. The immune activity of the IgG was verified and analyzed by SDS-PAGE,enzyme-linked immunosorbent assay(ELISA),affinity assay,Western blot assay,flow cytometry method (FCM)and immunofluorescence assay. The effect on the character of ovarian cancer cells was detected by cell counting kit-8 (CCK-8)assay,wound healing assay,transwell test and cell apoptosis assay. Results:The results demonstrated that the full human anti-Trop-2 IgG was successfully produced. The IgG specifically bound Trop-2 protein,and it could play an important role in the proliferation,migration and invasion of ovarian cancer cells as well as in inducing ovarian cancer cell apoptosis. When the concentration of IgG was at 400 μg/mL,only 55.95%(P < 0.01)of HO8910 cells survived. The amount of invasive cells in the experimental group was only 32.11% of those in the control group(P < 0.05). The wound healing rate of the experimental group was 24.71% while that of control group being 70.80%(P < 0.01),and the apoptosis rate was 2-folds higher than the control group (P < 0.05). Conclusion:The full human anti-Trop-2 IgG could specifically bind Trop-2 protein on the surface of ovarian cancer cells and restrain the malignant biological behavior of ovarian cancer cells. The IgG has potential applications in ovarian cancer targeting therapy.

Abstract:Objective:To investigate the effect of siRNA (small interference RNA) silencing Gankyrin on the behavior of human laryngeal carcinoma cell lines Hep-2. Methods:The expression of mRNA and protein levels of Gankyrin was detected by using qRT-PCR and Western blot before and after transfection,respectively. The cell proliferation was measured by CCK-8 assay. The apoptosis rate and cell cycle of Hep-2 cells were determined by flow cytometry. Cell migration was detected by wound healing assay and transwell assay. Matrigel assay was performed to observe cell invasive ability. The expression of p53 protein after down-regulation of Gankyrin was detected by Western blot. Results:Compared with the negative control group and the blank control group,the expressions of Gankyrin mRNA and protein were downregulated in the Gankyrin siRNA group,and the differences were statistically significant (P < 0.001). CCK-8 assay showed that the proliferation rate of the Gankyrin siRNA group was significantly decreased (P < 0.001). Flow cytometry showed that the apoptosis rate of the Gankyrin siRNA group[(7.70 ± 1.12)%]was significantly higher than that in the negative control group[(2.34 ± 0.32)%]and the blank control group[(1.82 ± 0.29)%],and the differences were statistically significant (P < 0.001). Compared with the two controls,G1 phase cells of the Gankyrin siRNA group were significantly increased,S phase cells were significantly decreased,and the difference was statistically significant (P < 0.001). Wound healing assay and transwell assay showed that the cell migration ability in the Gankyrin siRNA group was decreased significantly (P < 0.01). Matrigel assay showed that Gankyrin siRNA did not impact the invasive ability of Hep-2 cell (P > 0.05). The expression of p53 protein in the Gankyrin siRNA group was increased (P < 0.001). Conclusion:Down-regulation of Gankyrin inhibited the proliferation and migration ability of Hep-2 cell,which may be associated with the alteration of apoptosis,cell cycle and the expression level of p53.

Abstract:Objective:To design the miRNA expression cassettes targeting telomerase reverse transcriptase (hTERT)and to discuss the interfering effect on the expression of hTERT,the telomerase activity and the proliferation of K562,so as to provide a new gene therapy for chronic myeloid leukemia. Methods:miRNA expression cassettes targeting hTERT were constructed and transfected to K562 cells by liposome transfection. The telomerase activity and the expression of hTERT were detected by TRAP-silver staining and qPCR,respectively. Cell apoptosis rate was detected by Annexin V/PI. Results:After 48 h transfection,the expression of hTERT and telomerase activity were significantly reduced. The apoptosis rate of K562 cell was increased. Conclusion:The miRNA expression cassettes targeting hTERT can effectively interfere with the expression of hTERT,inhibit the telomerase activity and induce the apoptosis of K562 cells. RNAi technology based on the miRNA expression cassette is expected to become an efficient tool in molecular level for the treatment of chronic leukemia.

Abstract:Objective:To investigate the protective effect of reduced glutathione (GSH) on the hippocampal neuron of rats with severe acute pancreatitis (SAP). Methods:Seventy-two SD rats were equally assigned into 3 groups:the normal saline (NS) group,the SAP group and the GSH group. Each group included 3 subgroups:the 3,6 and 12 h groups. SAP models were established by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. Rats in the NS group were injected with normal saline instead of sodium taurocholate. Rats in the GSH group were peritoneally injected with GSH at the dosage of 25 mg/100 g body weight. Nissle stain was performed to evaluate the severity of brain injury. Hippocampal neuronal apoptosis was detected by TUNEL,and NF-κB p65 expression was detected by immunohistochemistry. Results:Pathological scores of pancreatic injury were significantly lower in the groups of GSH(3 h) and GSH(6 h) than those in the groups of SAP(3 h) and SAP(6 h) (P < 0.05). Hippocampal neuronal apoptosis indexes were significantly decreased (P < 0.05). The expression of NF-κB p65 was depressed mainly in the GSH(6 h)group. Conclusion:The hippocampal neuronal apoptosis in SAP rats with brain injury is reduced by GSH through an inhibition of NF-κB p65 expression in the hippocampus.

Abstract:Objective:To explore the effect of phosphorylation of neuronal nitric oxide synthase (nNOS) on regulation the neuropathic pain in spinal nerve ligation (SNL) model rats,and research the mechanism of nNOS phosphorylation in neuropathic pain. Methods:A total of 60 male SPF SD rats were randomly divided into 4 groups:the control group,only expose spinal nerve,not ligation;the experimental group,SNL model,KN93 (calcium/calmodulin dependent kinase Ⅱ inhibitor) intrathecal injection;the negative control group,SNL model,DMSO intrathecal injection;the model group,SNL model,didn′t receive any medicine. We determined mechanical pain threshold 1 day before operation,postoperative 1-5 days and 1-4 hours after sheath dosing. Western blot was performed to detect the expression level of calcium/calmodulin dependent kinase Ⅱ (p-CaMKⅡ),nNOS and pnNOS in lumbar spinal cord tissue. CoIP and immunofluorescence experiments was performed to test whether there was an interaction between nNOS and CAPON. Results:SNL led to mechanical pain threshold of rats reduced (P < 0.01),the expression of spinal cord tissue p-CaMKⅡincreased (P < 0.05),and the expression of p-nNOS decreased(P < 0.05). KN93 intrathecal injection reversed the trend above. There was an interaction between nNOS and CAPON,and the phosphorylation of nNOS reduced the strength of interaction between nNOS and CAPON. Conclusion:The phosphorylation of nNOS is involved in neuropathic pain induced by SNL,and p-CaMKⅡ make nNOS phosphorylation,reduced the strength of interaction between nNOS and CAPON. The nNOS signaling pathways can provide a new field of vision for neuropathy pain treatment.

Abstract:Objective:To study the mechanisms of erythropoietin in protecting cardiac function in diabetic rats. Methods:Forty male SD rats were randomly divided into the control group,the control-EPO group,the diabetic group and the diabetic-EPO group (n=10 for each). The diabetic model was induced by streptozotoein (STZ). In the control-EPO and the diabetic-EPO group,rats were treated with 1 000 IU/kg EPO by subcutaneous injection once per week for twelve weeks. At the seventh day after the last administration,echocardiography was conducted. Blood samples from tail vein were collected for blood glucose and red blood cell numbers measurements. After rats were sacrificed,myocardial tissues were collected,quantitative real-time PCR (RT-PCR) was used to detect the mRNA level of GRP78,SERCA2a and EPOR, and Western blot was used to detect the protein expression of GRP78,SERCA2a and EPOR. Results:Compared with the control and control-EPO group,in the diabetic group,ejection fraction (EF) and LV fractional shortening (LVFS) were decreased significantly (P﹤0.01),blood glucose was increased significantly (P﹤0.01),the mRNA level and the protein expression of GRP78 were increased significantly (P﹤0.01),the mRNA level and the protein expression of SERCA2a and EPOR were decreased significantly (P﹤0.01). EF and LVFS were increased significantly in EPO-treated diabetic rats (P﹤0.01). Diabetic rats receiving EPO administration showed a significantly decrease in the mRNA level and the protein expression of GRP78 compared with the diabetic group (P﹤0.01). EPO treatment significantly increased the mRNA level and the protein expression level of SERCA2a and EPOR (P﹤0.01). Conclusion:EPO treatment can improve the cardiac function,which might be related to the inhibition of endoplasmic reticulum stress,up-regulating of the SERCA2a and EPOR expression.

Abstract:Objective:The novel cytokine interleukin (IL)-29 has been reported to induce antiviral, antitumor, and immune responses. However,the effect of IL-29 on osteoclast differentiation remains unclear. This study aimed to explore the effect of IL-29 on osteoclast differentiation and function in RANKL-induced in RAW264.7 cells (osteoclast precursors). Methods:The expression of IL-28Rα (specific receptor of IL-29) on RAW264.7 cells was evaluated by flow cytometry. RAW264.7 cells were stimulated with RANKL and different doses of recombinant IL-29 for 5 d,then the osteoclast formation was evaluated by counting multinucleated cells for tartrate-resistant acid phosphatase (TRAP) staining. Furthermore,the effect of IL-29 on the RANKL-induced expression of osteoclastic genes (TRAP,CTR and CTSK) and relevant genes(TRAF6 and RANK) was detected by real time PCR. Results:IL-29 inhibited the osteoclast formation in a dose-dependent manner in RANKL-induced RAW264.7 cells,and the dose of 100 ng/ml IL-29 significantly reduced the number of TRAP positive multinucleated cells and mRNA expression of osteoclastic genes (TRAP,CTR and CTSK) and relevant genes(TRAF6 and RANK). Conclusion:The findings in the present study indicate,for the first time,that IL-29 could inhibit osteoclast formation and function.

Abstract:Objective:To analyze the expression profile variation of long non-coding RNA(lncRNA) in lung tissues of newborn mice with hyperoxia-induced bronchopulmonary dysplasia (BPD). Methods:We first successfully established a newborn mouse model of hyperoxia-induced BPD. We used lncRNA microarray technology to inspect differences of lncRNA expression profile between normal control lung and BPD lung,which helped us screen out the lncRNAs with differential expression after pretreatment of raw data. Results: Compared with the control group,there were 1 769 lncRNAs with differential expression which had more than 2-fold changes and significant differences (P < 0.05),of which 882 increased more than 2 times and 887 reduced more than 2 times;140 increased more than 5 times;71 reduced more than 5 times;28 increased more than 10 times and 2 reduced more than 10 times. Conclusion:lncRNA expression profile of hyperoxia-induced BPD was significantly changed in comparison with normal lung tissues. These lncRNAs with differential expression may be involved in the occurrence and development of BPD.

Abstract:Objective:To establish Vasohibin-2(Vash2) knockout mouse model with CRISPR/Cas9 gene targeting technology. Methods:A selected gene sequence of Vash2 was amplified with the primers of single-guide RNA (sgRNA),and then cloned into the plasmid pUC57-T7-GDNA. The Cas9 and sgRNAs were transcribed by T7 RNA polymerase in vitro. Transcribed gRNA/Cas9 mRNA was microinjected into the mouse zygote. The frame shifting mutation was validated by PCR and gene sequencing. Both the (F0 and F1 generation) knockout mice were analyzed. Results:The vector expressing sgRNA were successfully built. sgRNA and Cas9 mRNA were successfully transcribed and microinjected into mouse zygote. Five positive mice as the F0 generation were identified by gene sequencing. The No.5 mouse was selected to mate with wild-type mice,then achieved F1 generation were mated and produced F2 generation. The frame-shifted of Vash2 knockout mice (F2 generation) were evaluated by PCR and mutations were stably transmitted to the next generation. Conclusion:The Vash2 knockout mouse model was successfully built by Cas9/RNAsystemgene targeting technology,and it could be an efficient tool for Vash2 study.

Abstract:Objective:To breed and identify the offspring of positive miR-15a/16-1 gene knockout mice and analysis of their splenic cellular immune function. Methods:MiR-15a/16-1 knockout mice were paired in different ways. Genomic DNA was isolated from tails and analyzed by PCR. Complete blood analysis was performed in the blood which was collected from the orbit of mice. Analysis of cellular immune function in spleen was performed in miR-15a/16-1 gene knockout mice by FCM. Results:The positive rate with miR-15a/16-1 gene of filial generation mice met Mendelian genetic law. Compare with wild-type mice,CD19+ and NKG2D+ cells of spleen in miR-15a/16-1 gene knockout mice were all up-regulated expression detected by FCM,although there were not significant changes of frequencies of other surface markers. Complete blood analysis showed that lymphocytes increased. Conclusion:PCR methods can be used to identify the genotype of the miR-15a/16-1-/- mice,and to establish an animal experimental model to further study the important role of miR-15a/16-1. CD19+ and NKG2D+ cells of spleen in miR-15a/16-1 gene knockout mice were increased.

Abstract:Objective:As Th17 cells lack of specific surface marker,it is difficult to achieve highly purified population of Th17 cells. We sought to obtain highly purified population of Th17 cells by adjusting the concentration of cytokines and different cultivation methods. Methods:CD4+ CD44- naive T cells were separated from C57BL/6J mice by magnetic-activated cell sorting (MACS). Different inducing systems were used for testing the effect of different cytokines and culture methods. The ratio of Th17 cells was analyzed by flow cytometry,the expressions of IL-17,ROR-γt,IFN-γ,IL-4 and FoxP3 mRNA were measured by qRT-PCR method and the IL-17 concentration in cell culture supernatant was detected by ELISA. Results:Th17 cells were induced by TGF-β,IL-6 and IL-23,the ratio of Th17 increased by adding TNF-α,IL-1β,anti-IFN-γ and anti-IL-2. The use of U-type 96 wells plate was significantly better than the 24 wells plate in culturing Th17 cells. Th17 cells showed steady growth when cultured in U-type 96 wells plate and the ratio of Th17 cells was significantly increased [(91.85 ± 1.05)%]. The expressions of IL-17 and ROR-γt mRNA were up-regulated compared with that of Naive T cells (P < 0.001),however,IFN-γ,IL-4 and Foxp3 mRNA were decreased (P < 0.001). The concentration of IL-17 in cell culture supernatant was significantly increased (P < 0.001). Conclusion:The regulated method can generate a highly purified population (>90%) of Th17 cells.

Abstract:Objective:To study the molecular etiology of a hereditary hearing loss family associated with vestibular dysfunction by analyzing genetic pattern,phenotypic characteristics and pathogenic gene. Methods:Clinical data (medical history,audiological and vestibular function examination,as well as imaging examination) were collected. By target gene capture and massively parallel sequencing technologies,104 species and genes related to hereditary hearing loss and 3 microRNAs in probands of the pathogenic mutation were screened. Furthermore,we analyzed the gene coding region sequence of candidate mutations. Results:C.1458C>G (p.T352S) heterozygous mutations were found in the exon 11 of the COCH gene. Ⅰ2,Ⅱ1,Ⅱ5,Ⅱ9,Ⅱ13 and Ⅳ1 showed the same heterozygous mutation. Ⅱ11 and Ⅲ1 showed that the mutation was homozygous for this mutation,which means that there was no co-segregation,therefore,the possibility of pathogenic mutation in the family could be eliminated. No other mutations were found in the sequence of all 12 exons of the Ⅲ14 COCH gene. Conclusion:This family has an autosomal dominant disorder associated with vestibular dysfunction. However,the initial screening results were not found to be associated with the known deafness genes,including the COCH gene. Therefore,the molecular etiology of this family may be a new gene mutation.

Abstract:Objective:To investigate the changes of C1q tumor necrosis factor related protein 12 (CTRP12) in patients with obstructive sleep apnea hypopnea syndrome (OSAHS)with different severity. Methods:A total of 120 cases were selected from Southeast University Affiliated Hospital,Jiangyin City People’s Hospital,Chest Hospital Sleep Respiration Monitoring Center by polysomnography (PSG) monitoring,and divided into the OSAHS group and the non OSAHS group. The OSAHS group was divided into the light,medium and severe groups according to apnea hypopnea index (AHI),and each group was divided into the obesity,overweight and normal weight groups according to body mass index (BMI). CTRP12 level,AHI,the lowest oxygen saturation,BMI,blood lipid,blood sugar index in OSAHS patients were detected to understand the role of CTRP12 in OSAHS. Results: In BMI and OSAHS-matched patients of each group,the levels of CTRP12 before sleep and after wake up had no statistical difference;in BMI-matched groups,CTRP12 levels before sleep and after wake up of severe OSAHS patients were significantly lower than those of non OSAHS,light OSAHS,and medium OSAHS patients. CTRP12 levels in light and medium OSAHS patients with normal weight were significantly lower than those in non OSAHS patients;in OSAHS-matched groups,CTRP12 levels of obese patients before sleep and after wake up were significantly lower than those of patients with normal weight;plasma CTRP12 level of OSAHS patients was related to AHI,BMI,neck circumference,percentage of time when oxygen saturation lower than 90%(SLT90%),triglyceride,fasting blood glucose,lowest arterial O2 saturation(LSaO2),and mean arterial O2 saturation(MSaO2). The CTRP12 level was decreased when AHI,BMI,neck circumference,SLT90%,triglyceride,fasting blood glucose increased,and was increased when LSaO2 and MSaO2 increased. Conclusion:The level of CTRP12 was decreased when OSAHS patients with more severe disease and heavier body weight;OSAHS patients plasma CTRP12 level was negatively correlated with AHI,BMI,neck circumference,SLT90%,triglyceride,fasting blood glucose and positively related with LSaO2 and MSaO2.

Abstract:Objective:To investigate the efficacy and safety of tigecycline as a second-line therapy in the treatment of pulmonary infections in patients with hematologic diseases. Methods:When pulmonary infection can not be controlled in patients with hematologic diseases by first-line anti-infective therapy,especially existing etiological evidence or clinical suspicion of multi-drug resistant infection,we should consider tigecycline as an experiential second-line therapy in combination with other anti-infection antibiotics. The clinical data were analyzed to evaluate the efficacy and safety of this regimen. Results:A total of 50 cases of patients with hematologic diseases and pulmonary infection received this regimen. Eleven cases were cured,11 cases were in partial remission,16 cases were partial effection,and 8 cases were ineffective. The cure rate was 22% and the total effective rate was 76%. The onset time was 2-6 days (average 3.2 days). Seven cases died,and 5 cases attributed to uncontrolled infection. The most common adverse reactions in patients were vomit and diarrhea. The side effects were generally well-tolerated after symptomatic treatment. Conclusion:According to this study,the regimen of tigecycline as an experiential second-line therapy is effective and well-tolerated.

Abstract:Objective:To analyze the effect of nonuse of abdominal drainage in post hepatectomy of liver cancer. Methods:One hundred and thirteen patients who received resection of liver cancer were randomly designated to either experimental group (52 cases) or control group (61 cases). In the experimental group, the patients were not allowed to place the abdominal drainage;however, the patients in the control group underwent the use of the abdominal drainage conventionally. We observed the recovery of liver function after surgery, the time of the postoperative recovery, the length of hospital stay, the cost of hospital admission and postoperative adverse events and complications. Results:In the experimental group, the time of the postoperative recovery and the length of hospital stay were shorter than those in the control group, moreover, the cost of hospital admission was significantly reduced compared with the control group, all the differences reached statistical significance (P < 0.05). However, there were no differences of the recovery of liver function after surgery and postoperative adverse events and complications between two groups (P > 0.05). Conclusion:To the patients, nonuse of abdominal drainage after hepatectomy surgery is safe and effective,and this measure can accelerate the postoperative recovery of these patients. This measure can play an active role in ERAS (enhanced recovery after surgery) treatment.

Abstract:Objective:By using the advanced quality control tools,we analyzed and evaluated the risk of planning designs of gamma knife treatment prospectively,and improved the key events. Methods:A quality reconstruction team of physicists,oncologists and therapists conducted failure risk assessment analysis toward 200 gamma knife treatment plans and 100 treatment designs were performed by using failure mode and effect analysis (FEMA),and failure cause and countermeasures were analyzed by using causal factor analysis and fault tree analysis. Results:The risk priority number was significantly decreased[ average of (61.125 ± 8.125)%] after conducting improvement on 9 potential failure modes and 16 potential causes of failure. Conclusion:FMEA is a effective tool to improve the quality of gamma knife treatment planning designs. It helps reduce the potential risks as well.

Abstract:Objective:To evaluate the 3D T2 spectral adiabatic inversion recovery (SPAIR) Sampling Perfection with Application optimized Contrasts using different flip angle Evolutions(SPACE) sequence of elbow nerves in asymptomatic subjects by magnetic resonance neurography (MRN),and evaluate differences between different groups. Methods:MRN imaging was performed at 3.0 T MR. All 64 subjects underwent elbow nerve MRN covering the elbow region,including T2-weighted imaging with fat suppression and the isotropic 3D T2 SPAIR SPACE. For each volunteer,thin maximum intensity projection (MIP) and curved planar reconstruction (CPR) of coronal and sagittal position through the elbow nerves from proximal to distal were obtained from the same 3D dataset. Signal intensity (SI) and MRN images of elbow nerves were evaluated at three parts:upper arm,elbow joint and forearm. The increased SI of the three nerves on FS-T2WI and 3D T2 SPACE sequences were statistically compared to the surrounding muscle signals. Results:Images of high quality for all 64 volunteers were obtained by CPR. The neurography ratios of ulnar nerve by MIP from proximal to distal location were 92%,83% and 98%,and neurography ratios of median nerve and radial nerve were 81%,89% and 80%,as well as 94% and 95%,respectively. The increased SI of ulnar nerve on 2D FS-T2WI and 3D T2 SPACE sequences were 55% and 30%,respectively. Conclusion:The 3D T2 SPAIR SPACE sequence essentially improves the MRN of elbow nerves. SI abnormality of the ulnar nerve is more reliable on 3D SPAIR SPACE sequence.

Abstract:Objective:To forecast the monthly incidence of hepatitis A,B and C in China by state space model. To analyze the relationship between the accuracy of prediction and the cycle regularity of the incidence,and to provide a new approach to forecast the incidence of hepatitis. Methods:State space model was fitted with data of monthly reported cases of hepatitis A,B and C in China from 2005 to 2013. The SSM toolbox of MATLAB software was performed to construct the state space model,and the constructed model was applied to predict the monthly incidence of 2014. The evaluation index of the cycle regularity of the incidence was established by standardizing data. Results:The average relative error of prediction of hepatitis A,B and C was 17.03%,6.30% and 2.03%,respectively,and the mean periodic standard deviation of the standard data was 0.2143,0.1952 and 0.1724,respectively. Conclusion:The state space model can be fitted and performed to make a short-term prediction of the incidence of hepatitis. When the periodic standard deviation of the standardized data is smaller,the predicted result is more accurate.