Abstract:Objective:To explore the influence of motif chemokine ligand 7(CCL7) on pancreatic cell migration and invasion under hypoxic condition.Methods:We isolated cancer-associated pancreatic stellate cells(PSCs) by an outgrowth method,and analyzed the difference of chemokine expression in PSCs between hypoxia (1%) and normoxia (21%) by conducting a chemokine antibody array.By adding different concentration of recombinant protein CCL7(0 ng/mL,1 ng/mL,10 ng/mL,100 ng/mL) to the lower chamber containing 0.1% FBS DMEM,cell invasion assays were performed to determine the dose-dependent manner between CCL7 and invasion of pancreatic ancer cell(PCCs).By conducting Transwell invasion assays and lentiviral transfection,we were able to show that hypoxia induced secretion of CCL7 in PSCs,leading to increased invasion of PSCs.Results:Conditioned media from hypoxic PSCs enhanced PCCs invasiveness more intensely than that from normoxic PSCs(P＜0.05).Among various chemokiness,which were related to invasiveness,CCL7 was one of the overexpressed molecules in supernatant of hypoxic PSCs.CCL7 recombinant protein(0 ng/mL,1 ng/mL,10 ng/mL,100 ng/mL) induced pancreatic cells migration in dose-dependent manner.Pancreatic cells were shown to express CCR5 receptors for CCL7 detected by flow cytometry(P＜0.05).PSCs(Miapaca-2,colo357),which were chultured with PSCs supernatant,were involved an epithelial mesenchymal transition(EMT)cascade.Furthermore,the inbition of CCL7 by lentiviral transfecton suppressed the EMT.Conclusion:Hypoxic stellate cells of pancreatic cancer stroma induce PCCs invasion vis CCL7/CCR5 axis,of which the mechanism might inclued an epithelial mesenchymal transition (EMT) process.

Abstract:Objective: To investigate the Protective effect of Zhenwu Decoction on heart and renal tissues of type 2 CRS model rats. Methods: We built type 2 CRS model rats by combining ligation of left descending coronary artery and subtotal nephrectomy. Thirty-two rats were randomly divided into low, middle, high dose treatment and control groups. Treatment groups were given different concentration but same volume decoction for lavage and control group was treated with same amount of distilled water. Serum index, echocardiogram and immunohistochemical results were compared. Results: Compared with the control group, the high dose treatment group has reduced serum Cr and BUN level but higher excretion of urine indoxyl-sulfate (P<0.05). Serum Cr and BUN levels were lower in middle dose treatment group than the control group (P<0.05). All treatment groups had a higher amount of Lvpw(systolic),CO and SV than the control group. Addition,the high dose treatment group has increased diameter and volume of left ventricle. Masson staining, Collagen I and TGF-β immunofluorescensce results all demonstrate less fibrosis in heart and kidney tissues of the treatment groups than the control group. Conclusion: Zhenwu Decoction has protective effects on heart and renal tissue of Type 2 CRS model rats. This effect may be achieved by increase uremic toxin excretion and ameliorating fibrosis.

Abstract:Objective: To establish a vascular endothelial-specific overexpressed microRNA-155 transgenic rat model; and to observe its blood pressure, proteinuria phenotypes and changes after gestational load application. Methods: The exogenous miR-155 gene was inserted downstream of the VE-cadherin promoter specifical for vascular endothelial cells to construct the transgenic expression vector. After linearization of the vector, the miR-155 transgenic rats were established by zygote microinjection technique. The genotype of transgenic rats was identified by specifical primer PCR. Furthermore, tail-cuff method, biochemical detection and histological examination were used to preliminarily analyze the phenotypes of pregnant and non-pregnant female transgenic rats. Results: The miR-155 expressions in thoracic aorta, abdominal aorta, kidney and heart tissues of the vascular endothelial-specific overexpressed microRNA-155 transgenic rats were 2.3-fold, 3.3-fold, 2.9-fold and 3.3-fold higher than the wild-type rats，respectively. No significant differences were observed in blood pressure, urine protein, vascular wall or kidney structure between the miR-155 transgenic non-pregnant female rats and the non-transgenic rats. However, after pregnancy, the miR-155 transgenic female rats exhibited mildly elevated blood pressure, increased proteinuria, increased incidence of fetal growth restriction, disorderly structure of thoracic/abdominal aortic walls, tubular epithelial vacuolization, etc. Conclusion: After increase of gestational load, the vascular endothelium-specific miR-155 transgenic rats exhibited pre-eclampsia phenotype.

Abstract:Objective：To determine the effect and mechanisms of monocarboxylate transporter 1(MCT1)-mediated mitochondrial lactate shuttle on ventricular remodeling and heart failure induced by transverse aortic constriction(TAC) in mice.Methods：An animal model of cardiac hypertrophy was created by TAC in male C57BL/6 mice.Cardiac dimensions and functions were evaluated by echocardiography after 2 weeks，4 weeks， and 8 weeks following TAC，respectively.There were four groups：①Sham-operate group；②TAC 2 weeks group；③TAC 4 weeks group；④TAC 8 weeks group.Intracellular lactate and malondialdehyde(MDA)were measured by colorimetric，the proteins expression of cleaved-caspase 3 and MCT1 were detected by Western blot.Results：As echocardiography evaluation，the mice exhibited cardiac hypertrophy after TAC 2 weeks，heart failure after TAC 4 weeks，and severe heart failure after TAC 8 weeks.An increasing gradient of lactate and MDA concentrations，as well as cleaved-caspase 3 and MCT1 protein expressions were identified in the sham，TAC 2 weeks，and TAC 4 and 8 weeks groups.Besides，the concentrations of lactate and MDA and expressions of cleaved-caspase 3 and MCT1 were also found higher in the TAC 4 and 8 weeks groups than those of the sham and TAC 2 weeks groups.Conclusions：MCT1-mediated mitochondrial lactate shuttle plays an important role in the development of myocardial remodeling and heart failure.The molecular mechanisms may be through inducing oxidative stress and activating mitochondrial control of apoptosis.

Abstract:Objective：The effects of palmitic acid (PA) on the differentiation and function of mouse skeletal muscle stem cells was detected for the new medicine-sarcopenia.Methods：One hundred μmol/L PA was added to skeletal muscle stem cells.The morphological changes of the cultured cells were observed，the myosin heavy chains(MyHC) of the culture cells was determined using immunocytochemistry and the expression of the gene was determined by real time PCR. Oxygen consumption rates (OCR) was measured by XFe24 extra cellular flux analyzer. Levels of mitochondrial fusion and fission marks such as Mfn1，Opa1，Drp1，Fis1 were measured. Results：When the cells treated with PA，fluorescent expression of MyHC was decreased and the fusion rate of myotube also reduced. The oxygen consumption rate (OCR) in the presence of PA was decreased(P＜0.05). The fusion mark was not significantly changed，but mitochondrial fusion index(Mfn1-to-Drp1ratio) was significantly increased. Conclusion：The results showed that PA inhibited the differentiation of skeletal muscle stem cells and OCR of mitochondria. The effects of PA on myogenesis were carried out through decreasing the fusion of mitochondria.

Abstract:Objective：To establish the S100A16 gene knockout mouse model，which can be used for the study on its biologic function. Methods：To establish the S100A16 gene knockout mouse model via Cre/loxP system. PCR was used to identify the genotype of the offspring，the expression level of S100A16 mRNA was detected by qRT-PCR，and expression of S100A16 protein was detected by Western blot. Results：S100A16 gene knockout mouse model has been successfully established. Heterozygous mice were successfully bred and reproduced. So far，gene knockout homozygous mice were not found. Conclusion：The S100A16 mouse could be a useful model for the researches on its function，especially in obesity and insulin resistance.

Abstract:Objective：To observe the effects of metformin on the invasion and metastasis of undifferentiated thyroid cancer and to evaluate its underlying mechanisms. Methods：The undifferentiated thyroid cancer cell line SW1736 was treated with different concentrations of metformin. The invasive and metastatic abilities of SW1736 cells were measured by agar drop method and Transwell invasion and migration assay. Real-time PCR was used to detect the molecules mRNA involved in the process of epithelial-mesenchymal transition(EMT)，including vimentin，E-cadherin and transcription factors Snail1 and Slug. Results：Metformin inhibited the invasive and migration capacities of SW1736 cells in vitro. The inhibitory effects of metformin were dose dependent. Metformin significantly down-regulated the expressions of vimentin，Slug and Snail1 in SW1736 cells. Meanwhile，it stimulated the expression of E-cadherin，which highly expresses in epithelial cells. Conclusion：Metformin can promote the expression of E-cadherin by down-regulating the expression of Snail1 and Slug，thus inhibiting the process of EMT and decreasing the invasive and migration ability of thyroid carcinoma，which may provide a novel strategy for the treatment of thyroid neoplasms.

Abstract:Objective：To explore Forkhead box P2 (FOXP2) expression in GBM cell and its effect on invasion and migration of U87 and U251 GBM cells. Methods：RT-qPCR and Western blot were used to detect the mRNA and protein levels of FOXP2 in NHA and GBM cells. pEGFP-FOXP2 was constructed according to the manufacturer’s instructions and transfected into U87 and U251 cells. Wound healing assay and Transwell assay were used to test the invasion and migration ability of GBM cells. The changes of PI3K, Akt proteins was detected by Western blot. Results：The expression of FOXP2 was decreased in GBM cells both in mRNA and protein levels. The overexpression of FOXP2 significantly decreased the ability of invasion and migration of GBM cells and reduced the expression of PI3K and Akt proteins. Conclusion：FOXP2 is reduced in GBM cells and upregulating FOXP2 could increase invasion and migration of GBM cells through PI3K/Akt pathway, indicating that FOXP2 may serve as a tumor suppresser，which inhibits invasion of tumor cells during the development of GBM.

Abstract:Objective: To explore the promotive effect of FOXC1 on the invasion of glioblastoma U87 cells. Methods: The mRNA and protein expression levels of endogenous FOXC1 was examined in U87 and U251 cells as well as NHAs via qPCR and Western blot, respectively. The FOXC1-siRNA was transfected into U87 cells. The transfection efficacy of FOXC1-siRNA was examined using qPCR and Western blot. The wound healing assay and Transwell invasion assay were carried out to investigate the role of FOXC1 on glioma cell invasion. The changes of epithelial-mesenchymal transition(EMT)-related signaling pathway proteins and marker proteins in glioma cells were identified with Western blot. Results: Downregulating the expression of FOXC1 reduced the invasive ability of glioma cells, reduced the EMT-related signaling pathway proteins of glioma cells, increased the epithelial biomarker E-cadherin and decreased the mesenchymal biomarkers N-cadherin and Vimentin. Conclusion: The FOXC1 could effectively promote the invasion of glioma cells by inducing EMT.

Abstract:Objective: Centella asiatica (L.) Urb. is a traditional Chinese medicine that has many medical applications, including wound healing and anti-oxidation. Some traditional Chinese Medicine doctors have found that it has therapeutic effects for external use in the repigmentation of vitiligo and post-inflammatory hypopigmentation. This study was designed to evaluate the effects of madecassoside, a major bioactive component of C. asiatica, on oxidative stress in human melanocytes and its possible mechanism of action.Methods: Normal human melanocytes were treated with 0.01 mmol/L H2O2 and various concentrations of madecassoside (0, 10, 50, 100 μg/mL). Subsequently, the retraction velocity of melanocyte dendrites was assessed. Determination of mitochondrial membrane potential (MMP, ΔΨm) was performed by flow cytometry and intracellular calcium ([Ca2+]i) levels were measured. Alterations of mitochondrial ultrastructure were observed by transmission electron microscopy (TEM). Acridine orange (AO) staining was used to measure autophagy. The LC3-II/LC3-I ratio, an indicator of autophagosome formation, was analyzed by Western blot.Results: Under H2O2-induced oxidative conditions, madecassoside inhibited melanocyte dendrite retraction, improved MMP and reduced the accumulation of [Ca2+]i in a concentration-dependent manner. Observations by TEM showed that madecassoside attenuated the damage of mitochondria in human melanocytes caused by oxidative stress. Furthermore, autophagy activation was demonstrated by AO staining and an increased LC3-Ⅱ/LC3-Ⅰ ratio.Conclusion: These results demonstrate the antioxidative effect of madecassoside on human melanocytes subjected to oxidative damage is activated by autophagy. Moreover, madecassoside could be a promising treatment for vitiligo mainly caused by oxidative stress.

Abstract:Objective:Using isobaric tags for relative and absolute quantitation（iTRAQ），to find the region-typical membrane proteins of cochlear nuclues（CN）.Methods:The subjects are male D60 SD rats.The four regions CN,Inferior Colliculus(IC) ,Superior Oliver Com-plex(SOC) and the rest of the brain(Rest) ,were seperated. The region-typical proteins of CN were identified with iTRAQ. The functions of these proteins and the process of biological metabolism involved were discussed seriously through Uniprot.Results:Seventeen region-typical proteins of CN were identified.GO(Gene Ontology) pathway analysis indicates that they are mainly involved in biological regulation processes and development. Gal-1（Galectin-1），ANXA2（Annexin-A2）， and PRPH（Peripherin）were found to be related to the function of the nervous system through Uniprot.Gal-1 was involved in neural development and synapase plasticity，and has neuroprotective effects. ANXA2 had neurotrophic effects, and might be involved in the release of neurotransmitters. Highly expressed PRPH led to neurotoxi-city, and was involed in neurodegenerative disease.Conclusion: In CN, Gal-1, ANXA2 and PRPH proteins might be related to central audi-tory processing, and it provides theoretical basis for further researches on the pathogenesis of central auditory processing disorder.

Abstract:Objective: To explore the expressions of Nuclear Kappa B (NF-κB) and matrix metalloproteinase-9 (MMP-9) in placental tissues of pregnant rats with subclinical hypothyroidism and study the occurrence mechanism of threatened abortion. Methods: The expressions of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism and normal pregnant rats were detected by immunohistochemistry and Western blotting assay. Correlations between the expressions and the occurrence mechanism of threatened abortion of subclinical hypothyroidism were analyzed. Results: The positive rates of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism were 16.7% and 50% ,respectively,while in normal placental tissues were 83.3% and 100% ,respectively.The differences were significant(all P<0.05).The results of Western blotting assay were consistent with those of immunohistochemistry. Conclusion: The expression levels of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism have a high correlation with the invasion，and joint detection of the above indicators ，and it is instructional for judgement the occurrence mechanism of threatened abortion of subclinical hypothyroidism.

Abstract:Objective：To explore the effect on pulmonary fibrosis，cell apoptosis and expression of Fas/FasL in lung by blocking IL-17 activity in mice induced by bleomycin(BLM). Methods：Eighty C57BL/6 mice were randomly divided into the following 4 groups：sham group(SG)，BLM group(BG)，neutralizing antibody group(NG) and isotype-matched control antibody group(IG)，respectively. Three groups were received a single intratracheal instillation of 5 mg/(kg body weight) of BLM to induce pulmonary fibrosis，while SGs was administrated the equvalent sterile saline. NG and IG were injected through caudal vein with neutralizing rat antimouse IL-17 mAb，or control rat IgG every 3 days starting on day 1 before making model，SG and BG were received equvalent PBS alone. All mice were sacrificed after 28 days. Lung tissues were removed and used to evaluate the extent of pulmonary fibrosis by Masson staining and hydroxyproline contents measurement. Cell apoptotic rate and the expression of Fas/FasL in mice were detected through Flow cytometry and immunohistochemical method. Results：Compared with BG and IG，the pulmonary fibrosis degree of NG was decreased remarkably(P＜0.01). Hydroxyproline contents was reduced obviously(P＜0.01). Apoptotic rate and expressions of Fas/FasL were decreased significantly(P＜0.01). Conclusion：Pulmonary fibrosis was improved significantly after the endogenous IL-17 activity blocked，meanwhile，apoptosis and the expressions of Fas/FasL were both decreased. These data showed that antimouse IL-17 mAb ameliorate the pulmonary fibrosis induced by BLM and it is related to Fas/FasL apoptotic pathways.

Abstract:Objective: To investigate the incidence of BK virus (BKV) infection in renal transplanted recipients and analyze the risk factors of BKV infection. Methods: By measuring BKV DNA loads in urine and blood samples of the patients, 157 renal transplanted recipients who had received renal transplantation in our hospital from Dec. 2014 to Dec. 2015 were divided into 3 groups: BK viruria group, BK viremia group and normal group. Data of each group was then recorded, including age, gender, kidney primarydisease, type of dialysis, preoperative immune induction therapy，delayed recovery of graft function(DGF), HLA mismatch, acute rejection, dosage of postoperative corticosteroid, postoperative immunosuppressive regimen, postoperative pulmonary infection, et al. The risk factors of postoperative BKV infection were analyzed by logistic regression. Results: It was found that the incidence of BKV viruria was 35.03% (55 cases), that of BKV viremia was 11.46% (18 cases), and that of BKVAN was 0.63% (1 case). Univariate analysis by Logistic regression analysis found that postoperative immunosuppressive regimen (P=0.031) and postoperative hormone dose (P=0.037) were significantly related to BKV viruria. Multivariate analysis by Logistic regression analysis found that postoperative immunosuppressive regimen(P=0.041，OR=2.146) and postoperative hormone dose (P=0.042，OR=1.739) were significantly related to BKV viruria. Conclusion: Incidence of BKV viruria was higher than that of BKV viremia. There was only one recipient diagnosed BKVAN. Postoperative immunosuppressive regimen and postoperative hormone dose may be the risk factors of BKV viruria.

Abstract:Objective: To investigate the efficacy and security of low molecular weight heparin (LMWH) as prophylaxis against venous thromboembolism (VTE) after primary hepatocellular carcinoma (PHC) surgery. Methods: One hundred and five patients who received PHC surgery were randomly designated to either anticoagulation therapy group (48 cases) or non-anticoagulation therapy group (57 cases). In the anticoagulation therapy group, the patients were subcutaneously injected with LMWH 5 000 U per day from 2nd day to 7th day after operations; however the patients in the non-anticoagulant therapy group were not received the anticoagulant therapy of LMWH after operation. The incidence of postoperative VTE, intra-abdominal hemorrhage, incision bleeding and skin ecchymosis around incision of the two groups were counted. Coagulation function in the 6th day and hemoglobin on the 1st and 6th day after operations were collected. The total abdominal drainage of the two groups was calculated after operation. Finally, a statistical comparison was made between two groups. Results: In the anticoagulant therapy group, there was no patients suffering from VTE (0/48). However, there were 5 patients suffering from VTE in the non-anticoagulant therapy group (5/57), which indicated the incidence of VTE was 8.87%. LMWH significantly decreased the incidence of VTE (P=0.043). Furthermore, there are no significantly statistical differences on the aspects of intra-abdominal hemorrhage, hemoglobin variation, abdominal drainage, coagulation function, incision bleeding and skin ecchymosis around incision between the two groups(P>0.05). Conclusion: Our study indicates that PHC surgery often leads VTE, which could be effectively and safely treated by LMWH.

Abstract:Objective: To describe the levels of residual β-cell function in patients with newly-onset type 1 diabetes mellitus, and investigate factors that may be related. Methods: Data obtained from 105 newly-onset hospitalized type 1 diabetes mellitus patients in the First Affiliated Hospital of Nanjing Medical University from 2010 to 2015. Hemoglobin A1c, islet autoantibodies and HLA-A-DR haplotypes were tested. Mixed-meal tolerance test was carried out until the fasting blood glucose was lower than 10 mmol/L; blood glucose, insulin and C-peptide were measured during the test. The rates of peak C-peptide ≥ 0.2 nmol/L were calculated and logistic regression analyses were performed to explore the influence factors. Results:Eighty-night point five percent of patients had peak C-peptide ≥ 0.2 nmol/L. Logistic regression analyses suggested that factors including age of onset ≤18（OR 0.08，95% CI 0.01~0.90），diabetic ketosis or ketoacidosis onset（OR 0.08，95% CI 0.01~0.83），High-risk HLA-A-DRB1 haplotypes（OR 0.07，95% CI 0.01~0.61），counts of islet autoantibodies >2（OR 0.10，95% CI 0.01~0.87） were related to lower rates of peak C-peptide ≥ 0.2 nmol/L. Conclusion: We found that residual β-cell function exists in patients with newly-onset type 1 diabetes mellitus. These data reinforce the inadvisability of using C-peptide alone to differentiate between type 1 diabetes mellitus and other forms of diabetes. We also found that age of onset, diabetic ketosis or ketoacidosis onset, HLA-A-DRB1 haplotypes and islet autoantibodies were associated with β-cell function in patients with newly-onset type 1 diabetes mellitus.

Abstract:Objective: This study is aimed to explore the relationship between thyroid hormone and body composition in the elderly with euthyroid gland. Methods: Seventy-four euthyroid subjects with an average age 74.6±7.7 years old have been involved. Body weight, thyroid function and body composition have been measured. Results: Appendicular muscle mass (ASM) was positively associated with FT3(P=0.003). There is still a positive correlation between the two indexes after adjusting for age, sex and body mass index(BMI) (P=0.011). FT3 was positively correlated with lean subtotal(P=0.024). We found the same correlation between ASW and FT3 in female(P=0.043), but not male, after adjusting for age, sex and BMI.TSH was negatively correlated with appendicular fat mass and fat subtotal(P=0.039; P=0.038).None correlation between the two has been found after adjusting for confounding factors. Conclusion:Thyroid hormone is related with body composition in the elderly with euthyroid gland.

Abstract:Objective：To investigate the relationship of thyroid stimulating hormone(TSH) and metabolic syndrome(MS) with its components in different sexual subjects with normal euthyroid examination for preventing and curing metabolic syndrome and cardiovascular disease. Methods：We conducted a cross-sectional study of 10842 euthyroid subjects(male 7250，female 3592). Height，weight，blood glucose，blood pressure(SBP，DBP)，blood lipid(TC，TG，HDL，LDL)，uric acid，renal function and thyroid function，body mass index(BMI)，estimated glomerular filtration rate(GFR) were mesured. The relationship between TSH and MS was analyzed.Results：The prevalence of MS in male and female was 20.1% and 9.4%，respectively，and the TSH level in MS group was significantly higher than that in non MS group(male P=0.049；female P=0.009). Subjects were stratified according to quartiles of TSH level. The higher the TSH，the higher the prevalence of MS. With the increase of TSH，abnormal metabolism components also significantly increased (male P=0.05；female P=0.00). In males，TSH and TG，SBP was positively correlated(P=0.009，0.018). In females，TSH and SBP，DBP，TG，BMI were positively correlated(P=0.000，0.008，0，0)，and HDL was negatively correlated(P=0.022).Logistic regression analysis showed that the TSH level was the independent risk factor for MS in male subjects after adjusting for age，uric acid，blood glucose and blood lipids(P=0.043). Conclusion：In the subjects with normal euthyroid function，especially male， the increase of TSH level may be related to the increase risk of MS，and then increase the risk of cardiovascular disease.

Abstract:Objective：To explore the distribution of absolute natural killer(NK) cell count in peripheral blood in patients with chronic lymphocytic leukemia(CLL)，and evaluate correlation with other clinical indicators and its prognostic value. Methods：The information of a total of 126 patients with CLL treated in the First Affiliated Hospital of NJMU from 2005 to 2014 were collected in this retrospective study. Comparisons of NK cell count as continuous parameter in different groups were described using Mann-Whitney U test and the Kruskal-Wallis test. Kaplan-Meier method was used to survival analysis，and the Cox proportional hazards models were used for the estimation of prognostic factors. Result：The median absolute NK cell counts were 0.54×109/L(range：0.01×109～4.11×109/L) in all the 126 CLL cases. The most discriminative cut-points of NK cell counts at diagnosis was defined as 0.20×109/L. With a median follow-up of 44.5 months，patients with NK cell count ≥0.20×109/L had a 5-year overall survival(OS) of 71.9%，whereas patients with NK cell count＜0.20×109/L had a 5-year OS of 48.4%(P=0.031). Univariate analysis showed that low NK cell count was associated with inferior OS(P=0.019)，and multivariate analysis confirmed NK cell count as an independent risk factor(P=0.005). Conclusion：Absolute NK cell count in peripheral blood of CLL patients may reflect level of immunity，the reduction of NK cell count indicate poor prognosis.

Abstract:Objective：To evaluate the prognostic significance of perineural invasion (PNI) in patients with early cervical cancer. Methods：We reviewed clinical records and pathology slides of 306 patients with cervical cancer(FIGO IA2IIA2) after radical hysterectomy in Department of Gynecology，Jiangsu Provincial People′s Hospital From January 2008 to December 2015. Results：①The incidence of PNI in patients with early cervical cancer was 10. 5%(32/306). ②The incidence of PNI was correlated with tumor diameter，depth of invasion，lymphovascular spare invasion (LVSI) and lymph node metastasis(P＜0.05). ③The disease-free survival and overall survival of the PNI-positive group were significantly lower than those of the PNI-negative group. ④Univariate analysis showed that tumor diameter，PNI and clinical stage were correlated with the overall survival time of cervical cancer(P＜0.05). The tumor diameter was correlated with disease-free survival time (P=0.002). ⑤Multivariate analysis showed that PNI was not identified as an independent risk factor(P=0.154). Conclusion：The PNI is associated with tumor diameter，depth of invasion，LVSI and lymph node metastasis，and affect the prognostic of cervical cancer，but is not an independent risk factor.

Abstract:Objective：To explore related miRNAs to Mycoplasma pneumoniae(Mp) caused respiratory infections and immune reaction. Methods：Blood of children with Mp pneumonia(MPP) was collected. By miRNA microarray technology，miRNAs expression differences in lymphocytes were analysed between MPP and control group. RT-PCR was used to verify the accuracy of microarray results. Specific miRNAs associated with MPP were explored. Target gene software was used to predict the possible target genes，and RT-PCT was used to verify the mRNA expression difference. Results：Compared with normal group，in MPP group 105 miRNAs increased(with 30 more than 5 fold change，10 more than 10) and 133 decreased (with 133 less than 0.5 fold) (P＜0.05). Significantly differentially expressed miRNAs：miR-20a-3p，miR-100-5p，miR-93-5p，and let-7b-5p increased detected by RT-PCR，and were consistent with the array results. As potential target genes，Bcl-2，IGF-1R，PTEN，and TGFβR1 mRNA expression decreased. Conclusion：miRNAs differential expressed in blood lymphocytes in children with MPP，and the difference may be involved in infection and the immune response caused by Mp.

Abstract:Objective: To establish the evaluation model for risk factors of patients with breast cancer surrounding Beijing and Tianjin. Methods: The risk factors of patients with breast cancer were screened, then the new risk evaluation model was established based on these important factors. Diagnostic efficiencies of the new model and Gail model were compared by the calculation of area below ROC curve, and then the appropriate cut-offpoints of high,moderate and low risk were figured out. Results: The potential risk factors of breast cancer included age, body mass index(BMI) > 25, breast cancer-family history, long and bad emotions caused by undesirable life and the absence of emotion-adjusting ability, poor sleep, lifetime unpregnancy,smoking index >400,dense breast. Conclusion: The new model can provide a better prediction on patients’ risk of breast cancer.

Abstract:Objective：To investigate the epidemiologic characteristics of syphilis，establish an Autoregressive Integrated Moving Average Model(ARIMA) model for the prediction of monthly incidence of syphilis in Jiangsu and provide evidence for the prevention and control of the disease. Methods：We used monthly incidence data of syphilis in Jiangsu from 1995 to 2009 to establish the ARIMA model，and then evaluated the model. Results：ARIMA(1，1，0)，(2，1，0) models are the optimal models to predict the monthly incidence of syphilis in Jiangsu，the coefficients of recurrent model，seasonal first-order model，seasonal second-order model respectively are -0.579，-0.245，-0.357，statistics of t test respectively are 8.777，2.881，4.766. Correspondingly，the values of P respectively are 0.000，0.005，＜0.001. The model had favorably high precision，the predicting value was close to the true value，which was with in the 95% confidence interval of the predicting value. Conclusion：The ARIMA model was suitable to forecast the incidence of syphilis.ARIMA model could be used to predict the incidence trend of syphilis and provide evidence for the development of syphilis prevention and control measures.