Abstract:The pathogenesis process of glioma and normal astrocytes involved in a variety of cell ion channel alternations，different pathological types and levels，prognosis of gliomas have significant differences in ion channel alternations. Such alternations involve astrocytes to maintain their own cell cycle and intracellular environmental homeostasis，glioma malignant proliferation，metastasis，invasion，and etc. Previous studies have suggested that the above-mentioned cell ion channels can be used as a molecular target for the treatment of glioma chemotherapy，prevention of metastasis and other therapeutic usage that play an important role. The ion channel has a promising potential for the underlying point of study，molecular targeting of drug targets，and clinical molecular markers.

Abstract:Objective：To study the expression of CREB1 in glioma and its effect on the proliferation and invasion of human glioma cells. Methods：The expressions of CREB1 in different grades of glioma tissues were analyzed within the Chinese Glioma Genome Atlas expression Dataset（CGGA）. The correlation between CREB1 expression and clinical prognosis in glioma patients was analyzed by using CGGA Dataset. The small interfering RNA of CREB1（si-CREB1）were transfected into U251 and U87 glioma cells, the effect of down-regulation of CREB1 on cell proliferation and invasion were tested by CCK8 assay and Transwell assay. Results：The expression of CREB1 in higher grade glioma tissues was higher than that in lower grade tissues. High-expression of CREB1 associated with unfavourable prognosis in glioma patients. Down-regulation of CREB1 suppressed proliferation and invasion in glioma cells. Conclusion：The expression level of CREB1 relates with tumor pathological grade and clinical prognosis in glioma. si-CREB1 inhibits proliferation and invasion in glioma cells.

Abstract:Objective：To establish a method of patient derived xenograft（PDX）of human glioblastoma multiforme（GBM）for preclinical and biological research. Methods：GBM samples were freshly collected and planted subcutaneously. After the establishment of subcutaneous xenograft，tumor was taken out，dispersed and injected to the mice brain. HE staining was used to compare the morphological characteristics between primary tumor and PDX tumor，and Ki-67 was detected by immunohistochemistry to compare their proliferation activity. Results：We successfully established the PDX model，and found that the morphological characteristics of PDX by HE staining were similar with primary tumor，PDX remained the proliferation activity of primary tumor. Conclusion：The establishment of PDX model enabled us as preclinical tool for personalized treatment of GBM patients.

Abstract:Objective：To investigate the application of multimodal neuronavigation combined with intraoperative fluorescence guided technique in the high grade glioma of functional areas. Methods：Sixty patients were divided into the control group and the observation group. The control group received conventional craniotomy operation and the observation group operated by multimodal neuronavigation combined with intraoperative fluorescence guided technique. We collected tumor specimen at different distances and different staining region，and analyzed pathology and immunohistochemistry of these specimens. The total resection rate of tumor and nerve function disorder after operation was compared between two groups. The influence on prognosis of the patients was analyzed. Results：The observation group was successfully achieved preoperative function fusion and intraoperative navigation. Intraoperative fluorescence showed different staining regions. The pathological type of the two groups had no significantly difference. There was significant difference of high grade glioma resection rate and postoperative neurological function disorder and tumor recurrence of postoperative 3 mon between the two groups. Each index of immunohistochemical expressed significant changes in the trend at different distance of tumor specimens. The fluorescence staining area was consistent with the postoperative pathology. Conclusion：Multimodal neuronavigation combined with intraoperative fluorescence guided technique can improve the total resection rate of high grade glioma in brain function areas，and improve postoperative nerve function.

Abstract:Objective：To explore the value of dynamic susceptibility-weighted contrast-enhanced（DSC）MR imaging in differentiating glioma recurrence and cerebral radiation injury. Methods：Twenty-eight patients who had cerebral gliomas were treated with radiotherapy after operation，presenting newly abnormal enhancement. All the patients underwent routine MR，DSC perfusion. The value of rCBV and rCBF in the new enhanced lesion and the opposite white matter were measured and analyzed. Receiver operating characteristic（ROC）curve was used to find the best classification threshold，and then calculating its sensitivity，specificity and accuracy. Results： The relative rCBV and rCBF value in the recurrent lesion were 2.78 ± 1.01 and 1.89 ± 0.82，respectively， while in the radiation injured lesion were 0.76 ± 0.42 and 0.47 ± 0.31，respectively（all P＜0.05）. Analysis of ROC curve showed that：if the relative rCBV value≥1.88，the diagnosis specificity of recurrent glioma was 100.0%；if the relative rCBV value≤0.62，the diagnosis sensitivity of radiation injuries was 100.0%. The threshold of the relative rCBV value was 1.88，and the differential diagnosis sensibility，specificity，and　accuracy were 94.4%，100.0%　and　96.4%，respectively. If the relative rCBF value≥1.96，the diagnosis specificity of recurrent glioma was 100.0%；if the relative rCBF value≤0.45，the diagnosis sensitivity of radiation injuries was 100.0%. The threshold of the relative rCBF value was 0.85，and　the　differential diagnosis sensibility，specificity，and accuracy were 94.4%，90.0%　and 92.8%，respectively. Conclusion：DSC MRI is helpful to differentiate glioma recurrence and cerebral radiation injury.

Abstract:Objective：To investigate the expression of lncRNA BX357664 in human renal cell carcinoma （RCC） and its function in epithelial-to-mesenchymal transition （EMT） in Caki-1 and Caki-2 RCC cells. Methods： Downregulated LncRNA BX357664 was confirmed in RCC tumor tissues by previous lncRNA microarray. Real-time PCR was performed to analyze the expression of lncRNA BX357664 in 24 pairs of human RCC samples， Caki-1 and Caki-2 cells， respectively. Transwell migration and invasion assays were performed to detect the ability of migration and invasion in transfected Caki-1 and Caki-2 cells， respectively. Western blotting assay was carried out to identify the relative proteins in EMT process which affected RCC metastasis by lncRNA BX357664. Results： LncRNA BX357664 was downregulated in 24 pairs of RCC samples， Caki-1 and Caki-2 RCC cells， respectively （both P < 0.05）. Overexpression of lncRNA BX357664 suppressed migration （P < 0.01） and invasion （P < 0.01） by inhibiting EMT in Caki-1 and Caki-2 cells. The expressions of EMT related proteins were significantly changed， and the process of EMT was inhibited （P < 0.05）. Conclusion： LncRNA BX357664 was downregulated in RCC and could inhibit the ability of migration and invasion in Caki-1 and Caki-2 cells by blocking EMT.

Abstract:Objective：Previous genome - wide association study（GWAS）has identified that rs2252004 was associated with the susceptibility of prostate cancer. This study was aimed to investigate the molecular biological mechanisms underlying the effect of rs2252004 on tumorigenesis of prostate cancer. Methods：We obtained the genotype，methylation and gene expression data from The Cancer Genome Atlas（TCGA）database. The relationships between rs2252004 genotype（0，1，or 2）and the β-value or proportion of methylation at each CpG site（within 500 kb of rs2252004）and the expression level of involved genes were evaluated via linear regression. Results：The results showed that rs2252004 was significantly associated with the methylation level of three CpG cites located in FGFR2（cg25294906，P=0.031；cg03552039，P=0.042；cg16499947，P=0.049，respectively）. Furthermore，the expression of FGFR2 remarkably differed between 50 paired prostate tumors and adjacent normal tissues（P=9.3×10-11）. Besides，individuals carrying rs2252004 GT genotypes were significantly associated with decreased expression of FGFR2 than those with GG genotypes（P=0.007）. We also observed a notable different expression of FGFR2 between GT/TT genotype and GG genotype individuals（P=0.006）. Conclusion：Altogether，our results suggest that rs2252004 may regulate the methylation level of FGFR2，which contributes to influence FGFR2 expression，thus be involved in the tumorigenesis of prostate cancer.

Abstract:Objective：The aim of this study was to assess whether the transplantation of endothelial progenitor cells（EPCs）transfected vascular endothelial growth factor（VEGF）adenovirus protected kidneys from ischemia/reperfusion injury（IRI）in male rats，in order to further improve the paracrine mechanism of EPCs on renal IRI. Methods：Forty male Sprague-Dawley rats were randomly divided into four groups，including Sham-operated group，IRI-operated group，IPC-treated group and EPCs-treated group. Recombinant adenovirus encoding VEGF165 gene（Ad-VEGF165）was used to infect EPCs，and the transfection efficiency was identified. At 24 h and 72 h after reperfusion，serum samples were respectively collected for the serum urea nitrogen，serum creatinine after the transplantation of EPCs on renal IRI. Besides，kidney tissues were harvested to observe renal morphology changes. Subsequently，immunohistochemical staining detected the expression levels of CD31；vascular endothelial growth factor（VEGF），angiopoietin-1（Ang-1）and angiopoietin-2（Ang-2）expression levels in the kidneys were measured using western blot analysis at the indicated time points after reperfusion. Results：The results found that the serum urea nitrogen，serum creatinine and morphology were significantly improved，after EPCs transplantation with Ad-VEGF165. At 72 hours after reperfusion，expression levels of CD31，VEGF，Ang-1 and Ang-2 in the kidneys of EPCs-treated rats which was transfected by Ad-VEGF165 were markedly increased compared to rats subjected to IRI. Conclusion：The present work suggested that EPCs transplantation with Ad-VEGF165 could effectively treat kidney IRI in rats. In addition，the mechanism might be associated with VEGF paracrine function to further promote the expression of Ang-1，Ang-2 and other angiogenic factors after homing of EPCs，thus contributing to renal angiogenesis.

Abstract:Objective：To investigate whether knocking down PD - L1 expression can enhance the effects of DC vaccine to treat pancreatic cancer. Methods：PD-L1 expression in normal pancreatic tissues and pancreatic cancer tissue specimens were detected by immunohistochemistry in tumor specimen of 42 pancreatic carcinoma patients respectively. A Panc-1 cell line with low PD-L1 expression established by lentivirus mediated RNA interference targeting PD-L1 was named Panc- 1/PD-L1- RNAi. At the same time，negative control cells Panc-1/LV-Control and wide type Panc-1 cell line was used in this study. These three groups of pancreatic cancer cells were co-cultured with CD8+ T cells and mature DC，and then IFN-γ production was detected by ELISA. Pancreatic cancer tumor-bearing hu- SCID mice model was established. DC vaccination was performed to investigate tumor inhibition effects. Results：Higher expressions of PD - L1 were detected in pancreatic carcinoma than in normal pancreatic tissue（P＜0.001）detected by immunohistochemistry examination. In the T cell reaction study，IFN-γ production is much higher in the PD-L1 knocking down group than that in the other two control groups（P＜0.001）. In vivo，PD-L1 knocking down combined with DC vaccination could significantly prevent tumor growth in the hu-SCID model. Conclusion：PD-L1 is expressed higher in pancreatic cancer than in normal pancreatic tissue. Suppressing PD-L1 expression in cancer cells during DC vaccination might be a more efficient strategy in immunotherapy for pancreatic cancer

Abstract:Objective: To explore the effects of CD146 overexpression in ovarian cancer cell line SKOV3 on the tumorigenesis in nude mice. Methods: The lipofection-mediated transfection in SKOV3 cells was performed and transfectants were screened by G418 resis- tance to obtain positive clone cells that overexpressed CD146 protein confirmed by Western blot. MTT assay and colony formation assay on soft agar were used to evaluate the proliferation and colony formation ability of SKOV3 cells that overexpressed CD146, respectively. These positive cells were subcutaneously injected into the nude mice, and the tumorigenesis of SKOV3 was observed and recorded every week until the sacrifice of the nude mice. The tumor weight and volume were measured and immunohistochemical staining was used to detect CD146 expression in the tumor tissue of nude mice. Results: After lipofection and G418 screening, the positive clone cells over- expressed CD146 were selected. CD146 overexpression significantly inhibited cell proliferation and decreased colony formation ability in SKOV3 cells in vitro, and also inhibited the tumorigenesis of SKOV3 in the nude mice. The tumor weight and volume from the nude mice injected subcutaneously with overexpressed-CD146 SKOV3 cells were respectively lighter and smaller than those of the control （P < 0.05). Immunohistochemical staining showed that tumor tissues from nude mice injected with overexpressed-CD146 SKOV3 cells still could express high level of CD146 protein. Conclusion: CD146 overexpression can inhibit cell proliferation in vitro and tumorigene- sis of SKOV3 in nude mice. CD146 may be a potential marker for ovarian cancer prognosis.

Abstract:Objective：To analyze the expression of costimulatory molecule B7-H3 in patients with acute myeloid leukemia（AML），and explore the clinical significance of the expression of B7-H3. Methods：The expression of B7-H3 protein was detected by flow cytometry（FCM）and the expression of B7-H3 mRNA was detected by RT-PCR. We analyzed the relationship between the expression of B7-H3 and some clinical factors. We evaluated the progression-free survival（PFS）and overall survival（OS）between the B7-H3 positive group and the B7-H3 negative group. Results：The results demonstrated that B7-H3 was weakly expressed on bone marrow of AML patients，and RT-PCR also detected mRNA of B7-H3. No B7-H3 positive cells were detected in the normal human bone marrow. B7-H3 was detected in 28 of the 66 cases（42％）. However，among 66 AML cases，no significantly higher frequency of B7-H3 positive cases was found in the male or female group（P=0.924）. Neither was observed between patients who were older than 60 years and those less than 60 years（P=0.541）. The B7-H3 positive expression was of no significant within the higher WBC subset（≥ 30×109/L）（P=0.310）. There was significant differences between favorable karyotype subset and unfavorable ones（P＜0.001）. There was also statistical difference in PFS（7.4 mon vs. 15.2 mon）and OS（9.8 mon vs. 17.8 mon）between the B7-H3 positive group and B7-H3 negative group，respectively（P＜0.05）. Furthermore，in the AML-M2 subtype，the expression of B7-H3 on the unfavorable karyotype group（8 cases）was higher than the favorable group（29 cases，30.05% ± 23.10% vs. 10.08% ± 7.07%，P＜0.001）. There was coexpression between B7-H3 and CD34 of blast cells. The expression of B7-H3 in the CD34 positive group was higher than that in the negative group（20.90% ± 19.70% vs. 9.63% ± 7.21%，P=0.034）. Conclusion：The expression of B7-H3 in AML has relationship with the expression of the karyotype. The B7-H3 positive patients have the worse prognostic. The research of B7-H3 may be a new prognostic marker and provide insights into the leukemogenesis of acute myeloid leukemia.

Abstract:Objective：To investigate the prognostic value of microRNA-107（miRNA-107）for patients with penile-sparing surgery.Methods：The clinical data of 34 patients with penile-sparing surgery were collected from June，2009 to June，2014 in the first affiliated hospital of Nanjing Medical University. Kaplan - Meier method was used to analyze the survival data. Univariable analyses and multivariable analyses by Cox regression were performed to analyze the correlation between the potential factors and the prognosis.The factors included age，marital status，history of smoking，histological grade，TNM stage，tumor position，and miRNA - 107 expression.Results：We found that miRNA - 107 expression was associated with histological grade（χ2=3.948，P=0.047）. Univariable analyses showed that higher histological grade（hazard ratio，HR=5.537，P=0.007）and elevated expression of miRNA-107（HR=7.651，P=0.001）significantly correlated with worse prognosis. Multivariable analyses demonstrated that higher miRNA - 107 expression was related to unfavorable prognosis for patients（HR=10.391，P=0.003）. Conclusion：miRNA-107 is an independent prognostic factor for patients with penile-sparing surgery，and it could be considered as an important biomarker.

Abstract:Objective：Through the difference of clinical and endoscopic feature，to explore the diagnosis between the patients with MALT lymphoma and diffuse large B cell lymphoma in the gastrointestinal tract. Methods：A total of 119 patients with MALT lymphoma and diffuse large B cell lymphoma in the gastrointestinal tract proven by pathology from January 2009 and to July 2016，were retrospectively analyzed. Results：Of the 119 cases，The male-to-female ratio of MALT lymphoma and DLBCL were respectively 1.73：1，≈1 and the median age was 55 years. However，MALT lymphoma and DLBCL were both mainly involved in the stomach，but the most frequent site of MALT lymphoma was body of the stomach，as to DLBCL，antrum of the stomach was the most frequent site. The most common symptom of MALT lymphoma and DLBCL was both abdominal discomfort，followed by weight loss，gastrointestinal bleeding and so on. The diagnosis rate of gastrointestinal endoscopy was 74.8%. Patients with MALT lymphoma diagnosed by EMR/ESD were more than patients with DLBCL（P=0.006），and the two groups diagnosed by the way of biopsy and surgical had no statistical difference（P > 0.05）. Endoscopic performance mainly presented as ulcerative type，mucosal uplift type，superficial type，tumer-like and polypoid type. The most common type of the two groups was both ulcerative type. MALT lymphoma was 73.2% in stageⅠ ~ Ⅱand DLBCL was 42.3% in stageⅠ ~ Ⅱ. There was statistically significant difference between the two groups（P=0.001）.The patients with higher level of LDH were significantly more common in DLBCL than in MALT lymphoma（P=0.002）. In addition，the positive rate of MALT lymphoma group and DLBCL group respectively were 53.1%，46.5%. Conclusion：Patients with MALT lymphoma and DLBCL were varied in clinical manifestations. The rate of endoscopic diagnosis was high and the ways of endoscopic diagnosis included biopsy，EMR or ESD of large organizations. The most common endoscopic lesion was ulcerative type. Patients with MALT lymphoma diagnosed by EMR/ESD were more than patients with DLBCL，and clinical staging of MALT lymphoma are early and DLBCL are advanced. The patients of high levels of LDH in DLBCL are more than MALT lymphoma.

Abstract:Objective：To observe the association of copeptin levels with coronary atherosclerotic lesions in postmenopausal women with impaired glucose tolerance（IGT）. Methods：Eighty postmenopausal women （aged 56-59 y）with the onset of IGT（fasting blood glucose，i.e. FBG＜7.0 mmol/L，7.8 mmol/L≤2-hour postprandial blood glucose，i.e.2hPBG＜11.0 mmol/L）were divided into group A（copeptin < 5 pmol/L）and group B（copeptin > 20 pmol/L），according to serum copeptin levels at baseline，40 cases in each group. The discrepancy of coronary CTA stenosis scores was analyzed between the two groups at baseline and after 6-month follow-up. Results：①At baseline，the difference was statistically significant in copeptin levels（P < 0.05），but not in weight，FBG，2hPBG，fasting serum triglyceride（TG），total serum cholesterol（TC），serum low-density lipoprotein cholesterol（LDL-C），mean arterial pressure，serum creatinine and age between group A and group B（P＞0.05）.②After 6-month therapeutic lifestyle adjustment as well as oral administration of aspirin（100 mg qd）and rosuvastain（10 mg qd），copeptin level，coronary CTA stenosis scores，FBG and 2hPBG of group B was statistically significantly higher，compared with group A，respectively（P < 0.05）.Furthermore，some cases developed diabetes or coronary heart disease（CHD）in group B，while not in group A. Conclusion：On the basis of the influence of the atherosclerotic risk factors of IGT and postmenopausal female，high plasma copeptin level will further promote the occurrence and deterioration of coronary atherosclerotic lesions.

Abstract:Objective：The primary aim of this study was to compare the incidence of acute adverse reactions and dosimetric parameters of postoperative cervical cancer patients with intensity modulated radiotherapy（IMRT）and volumetric modulated arc therapy（VMAT），and to provide the reasonable selection of the cervical cancer patients who had needed postoperative radiotherapy. Methods：In our study，a total of 100 cervical cancer patients who needed postoperative radiotherapy were respectively treated with IMRT or VMAT，including 50 patients in the IMRT group and 50 patients in the VMAT group. The acute radioactive adverse reactions，dose of organ at risk（OAR），homogeneity index（HI），conformity index（CI），the number of monitor units（MUs）and treatment times were compared between the two groups. Results：The incidence of acute radioactive enteritis in the VMAT group was obviously lower than the IMRT group（38% vs. 64%，P < 0.05）. Compared with the IMRT group，the incidence of upper gastrointestinal tract reaction significantly decreased in the VMAT group（20% vs. 6%，P < 0.05）. The small intestine V40 and rectum V40 of the VMAT group was markedly lower than those of the IMRT group（P < 0.01）. However，rectum and small intestine V20 and V30，as well as bladder V20，V30，and V40 showed no statistical difference between the two groups（P > 0.05）. Compared with the IMRT group，the CI of VMAT group was significantly improved（P < 0.05），while there were no statistical differences of the HI between the two techniques（P > 0.05）. The MUs and treatment times of the VMAT group compared with those of the IMRT group were significantly decreased by 50% and 54%，respectively（P < 0.01）. Conclusion：VMAT is superior to IMRT in many aspects. Cervical cancer postoperative patients choose VMAT treatment could reduce the acute radioactive adverse reactions，decrease the dose of OAR，and shorten the treatment time of radiotherapy to improve the tolerance of patients with postoperative radiotherapy.

Abstract:WHO officially identified clear cell papillary renal cell carcinoma（CCPRCC）as a new type of renal cell carcinoma in 2016. This tumor type can be encountered in a sporadic setting or can occur in end-stage renal disease or even VHL syndrome，with relatively characteristic histopathological morphology，immunophenotype and molecular genetics. It needs to be identified with clear cell renal cell carcinoma，papillary renal cell carcinoma or renal carcinomas associated with Xp11.2 translocations. In view of the biological behavior of CCPRCC is relatively inert or low-grade malignant，therefore accurate diagnosis plays an important role in clinical treatment and judging prognosis.

Abstract:The chorionic bump is a recently described，uncommon abnormality of the first-trimester gestational sac，which has been considered a　risk factor for nonviability in pregnant patients. The etiology，pathology，diagnosis and clinical significance of the chorionic bump　are reviewed in this paper to help clinicians to provide patient counseling and make a comprehensive assessment of the possible pregnancy outcome.