Abstract:Objective：To investigate the effects of tumor necrosis factor-like attenuated apoptosis factor（TWEAK）mediated by Fn14-PI3K-Akt signaling pathway on the proliferation，migration and angiogenesis of human umbilical cord blood derived human endothelial progenitor cells（hEPCs）. Methods：hEPCs were isolated and identified in vitro. The hEPCs were treated with Fn14 siRNA and LY294002（LY），a specific inhibitor of PI3K. The cells were divided into the control group，the TWEAK treatment group，the TWEAK + Fn14 siRNA blocking group and the TWEAK + LY treatment group. Cell proliferation was measured by CCK-8 assay，cell migration assay was performed in Transwell chamber，and angiogenesis ability was evaluated by Matrigel tubule formation assay. The expressions of p-Akt and T-Akt in cell lysate were measured by Western blot. Results：The hEPCs cultured in vitro showed that TWEAK significantly promoted the proliferation and migration of hEPCs and enhanced the vascularization ability of hEPCs. However，the proliferation，migration and angiogenesis ability of hEPCs in the TEWAK + Fn14 siRNA group and the TWEAK + LY groups were significantly decreased. The expression of p-Akt in the TWEAK group was significantly higher than that in the control group，while the expression of p-Akt in the TWEAK + Fn14 siRNA group and the TWEAK + LY group was significantly lower than that in the TWEAK group. Conclusion：TWEAK can regulate the angiogenesis of hEPCs and its angiogenesis may be restricted by Fn14-PI3K-Akt signaling pathway.

Abstract:Objective：To identify Alu RNA expressed in oxidative-stressed A549 cells and to construct eukaryotic expression plasmids for Alu RNA and its two kinds of loss-of-function mutants so as to study the effect on oxidative stress in cells with overexpression of Alu RNA and its mutants. Methods：Alu RNA was amplified with RT-PCR from H2O2 treated A549 cells. pcDNA3.0-Alu（pAlu），pcDNA3.0-Alu-left arm（pAlu-LA）and pcDNA3.0-Alu G25C（pAlu-M）were constructed by directional cloning and point mutation. After recombinant transfection，reactive oxygen species（ROS） produced in transfected A549 cells was detected by DCFH-DA. Results：Alu fragment was successfully amplified from stressed A549 cells. Restriction analysis and DNA sequencing proved that recombinant eukaryotic expression plasmids of pAlu，pAlu-LA and pAlu-M were constructed as expected. pAlu or pAlu-LA transfection promoted ROS production significantly in A549 cells，while pAlu-M transfection induced ROS production was markedly decreased as compared with pAlu transfection. Conclusion：The eukaryotic expression plasmids for Alu RNA and its loss-of-function mutants were successfully constructed from oxidative-stressed A549 cells. Data in vitro suggested that Alu RNA promoted ROS production in A549 cells with translational regulation.

Abstract:Objective：To investigate the biological effects of corticotomy-induced micro-environment around orthodontic teeth on macrophages. Methods：A total of 60 male Wistar rats were randomly divided into the experimental group（TM+CO）and control group（TM）with each group of 30 rats. The rats in each group were sacrificed after 3，5，7，14 and 21 days for tissue collection and assessments. Micro-CT examination was performed to acquire bone mineral density（BMD）around the upper left first molars，to analyze the change of bone remodeling. Morphological changes of periodontal tissues and the expression of CD68，CD11b，CD86，CD163-positive cells were determined by immunohistochemistry. A total of 112 male C57BL/6 mice of eight-week old were randomly divided into the corticotomy group and the vehicle group. The mice in each group were sacrificed after 3，5，7，14，21，28 and 42 days for tissue collection and co-culture. The relative mRNA expression of CD11b，CD68，IL-6，IL-1β，IL-10 and Arg-1 were respectively evaluated by RT-PCR. The protein change of p65 and STAT3 phosphorylation were detected by Western blot. Results：The value of BMD in both groups decreased significantly at the early stage and it was more obvious in the TM+CO group（P < 0.05）. Meanwhile，the immunohistochemistry data showed that the expressions of CD68，CD11b，CD86，CD163-positive cells in the TM+CO group were all significantly increased compared with those in the TM group at day 3（P < 0.05）. The real-time PCR analysis showed that the mRNA expression levels of CD11b and CD68 in macrophages were significantly higher in the corticotomy group than those in the vehicle group（P < 0.05）；they increased from day 3 to day 14 and then declined. Moreover，the mRNA expression levels of M1 marker，TNF-α，IL-1β and M2 marker，IL-10，Arg-1 were significantly higher in the corticotomy group compared with those in the vehicle group（P < 0.05）；they increased up to day 14 and then declined. Western blot analysis showed that the level of p65 was upregulated after co-culture with condition medium of maxilla in the corticotomy group early on day 5 and day 7，while the level of phosphorylated STAT3 was upregulated on day 7 and day 14 when compared with those in the vehicle group（P < 0.05）. Conclusion：The activation of NF-κB and JAK-STAT signaling pathways may play an important role in corticotomy induced infiltration and polarization of macrophages and thereby influenced bone density and consequent orthodontic tooth movement.

Abstract:Objective：Calcium phosphate cement（CPC）scaffold is widely used in bone tissue engineering due to its good biocompatibility and osteoconduction properties. However，it needs further improvements to widen its application. In this study，CPC scaffold with gold nanoparticles（AuNPs）were used to promote the proliferation and osteogenic differentiation of dental pulp stem cells（DPSCs）. Methods：The morphology of AuNPs was detected by transmission electron microscope（TEM）. The cells were seeded on CPC，and cultured in media containing 5 μg/mL AuNPs. Those seeded on CPC and cultured in normal media was set as control. The cell adhesion was detected by fluorescent stain 4 h after seeding. The cell proliferation was detected by CCK-8，and the alkaline phosphatase（ALP）activity was measured. Alizarin red staining（ARS）was used to detect the mineral synthesis. Results：TEM image showed that AuNPs were spherical with 20 nm in average diameter. Fluorescent images showed no significant difference in cell adhesion between the two groups. Significant difference in cell proliferation was detected at 14 days（P < 0.05）. ALP activity of the AuNPs media group was significantly increased（P < 0.05），and the mineralization formation of DPSCs was also increased compared with that of cells in normal media（P < 0.05）. Conclusion：It was concluded that CPC scaffold with AuNPs can promote the proliferation and osteogenic differentiation of DPSCs.

Abstract:Objective：To establish the dibutyl phthalate（DBP）poisoning mouse model and discuss the protective response of sulforaphane（SFN）. Methods：A total of 30 male mice with age of 6 weeks old were randomly divided into the solvent control group（soybean oil group），the SFN group［0.75 mg/（kg·d）］，the DBP group［500 mg/（kg·d）］，the DBP with SFN low dose group［0.5 mg/（kg·d）］，the DBP with SFN middle dose group［0.75 mg/（kg·d）］and the DBP with SFN high dose group［1 mg/（kg·d）］with 5 mice in each. After continuous feeding drugs for 2 weeks，all mice were sacrificed and anatomized，the testis weight，anogenital distance（AGD），AGD/weight and the organ coefficients of testis were measured. In addition，the testicular histopathological changes of mice were observed and one side of epididymis was used to analysis the sperm counts，viability and deformity rate. Results：The DBP group presented with obvious lower AGD/weight，sperm counts，viability and higher deformity rate，significant testicular histopathological changes compared to the soybean oil group（P < 0.05）. However，all the test results in the DBP with SFN low dose group were superior to that of the DBP group（P < 0.05）and the testicular histopathological changes were improved significantly. Conclusion：SFN could alleviate the reproductive toxicity induced by DBP significantly and this provides an experimental animal model for further studying the toxicity of DBP and the protective mechanism of SFN.

Abstract:Objective：To study the influence of multiple myeloma cells（MMCs）on the secretion of tissue-type plasminogen activator（tPA）and tissue-type plasminogen activator（PAI-1）by the cultured human umbilical vein endothelial cells（HUVEC）to confirm the fibrinolytic activity as well as the causes and mechanisms of thrombosis in multiple myeloma（MM）. Methods：Human MM cell line U266 was co-cultured with HUVEC for 24 h，48 h and 72 h，with the same period of HUVEC cultured alone as control as well as MMCs. The expressions of HUVEC，MMCs tPA，and PAI-1 mRNA in those groups were detected by polymerase chain reaction（PCR），tPA and PAI-1 protein levels in the supernatant were detected by ELISA. Results：No expression of tPA and PAI-1 protein and mRNA in the culture supernatants of MMCs was detected by both ELISA and PCR. The expression level of tPA protein in the co-culture system by ELISA was significantly（P < 0.05）higher than that in the control group，as well as the PAI-1 protein level（P < 0.05）. There was a positive correlation between the PAI-1 and tPA protein level in the control group（rs=0.80，P=0.01）and in the co-culture system（rs=0.88，P=0.002）by ELISA. The levels of t-PA and PAI-1 protein were positively correlated with the time in the HUVEC cultured alone group（rs=0.90，P=0.001；rs=0.90，P=0.001）and the co-culture system（rs=0.95，P＜0.001；rs=0.84，P=0.004）significantly by ELISA. The difference of the expression level of tPA mRNA between the two groups was not significant（P > 0.05），as well as the expression level of PAI-1 mRNA（P > 0.05）. Conclusion：MMCs can promote the secretion of tPA and PAI-1 protein from HUVEC. On the premise of ensuring the cells viability，the secretion of both proteins increased along with the extension of incubation time，and there was a certain correlation between the tPA and PAI-1 protein level. However，MMCs had no obvious effect on the expression level of tPA and PAI-1 mRNA.

Abstract:Objective：To explore the effects of KLF7b on embryo development of zebrafish. Methods：Wild-type KLF7b mRNA and KLF7b acetylated site mutant mRNA were transcripted in vitro. Specific mRNA and morpholino oligonueleitides（MOs）were used to overexpress or site mutate or knockdown KLF7b by microinjection at 1~2 cells stage. The phenotypes of embryos were observed at different stages and the expressions of marker genes were tested by quantitative RT-PCR. The bone development of zebrafish was detected by calcein staining. Results：KLF7b overexpression had no effects on embryo development of zebrafish. KLF7b knockdown or acetylated site mutation caused embryonic abnormalities including pericardial effusion，the developmental malformations of somite and head. The marker genes of muscle were down-regulated. Conclusion：KLF7b may affect the development of zebrafish via its own acetylation.

Abstract:Objective：To discuss donors’ and recipients’ risk factors for the short-term outcomes of kidney transplanted recipients from donation after citizen’s deceased（DCD）. Methods：We retrospectively analyzed the information of donors and recipients at the Kidney Transplantation Center，Jiangsu Province Hospital from January 1，2015 to Oct 31，2016. We compared the risk factors of delayed graft function（DGF）and 1-year estimated-glomerular filtration rate（eGFR）of recipients. Results：The DGF rate of kidney transplanted recipients from DCD was 28.2%. On univariate analysis of DGF，female in donor（P=0.019），body mass index（BMI）of donor（P=0.027），warm ischemia time（WIT，P=0.044），and the history of cardio-pulmonary resuscitation（CPR，P=0.042）were showed significant differences. On multivariate analysis，age of donor（P=0.027）and WIT（P=0.042）were independent risk factors for the incidence of DGF. Recipients with DGF had more hospital stay（P=0.020），more hospitalization expenses（P=0.012）and more incidence of acute rejection（AR，P＜0.001），compared with recipients without DGF. On multivariate analysis of 1-year eGFR of recipients，age of donor（P=0.005）and the complications of AR（P=0.034）were independent risk predictors. Conclusion：Age of donor and WIT were independent risk factors for the incidence of DGF of recipients. Age of donor and the complications of AR were independent risk predictors for 1-year eGFR of recipients. These factors should be considered while evaluating potential donors.

Abstract:Objective：To assess the mechanism of the variation of serum uric acid in obese patients with different body mass index（BMI）. Methods：Retrospective analysis was performed in 269 obesity patients with BMI≥28 kg/m2 from the authors’ affiliated hospital. According to their BMI，they were respectively divided into three groups，including the mild，moderate and severe obesity groups. Serum uric acid，anthropometric and metabolic characteristics of patients were measured. Each group was compared and the correlation between serum uric acid level and other index was analyzed. Results：The serum uric acid levels in all the obesity patients were increased with the increasing BMI，and which in male obesity were higher than that in female obesity（P < 0.05）. The prevalence of hyperuricemia in these obese patients also increased with BMI，which in male and female severe obesity patients were both more than 70%. Serum uric acid in all obese patients was positively correlated with BMI，fasting insulin，HOMA-IR and IL-6. Conclusion：Serum uric acid levels and the prevalence of hyperuricemia were both increased with the increasing BMI. Hyperuricemia is positively correlated with BMI，insulin resistance and inflammatory factors.

Abstract:Objective：To investigate the expression of kinesin family member 4A in colorectal cancer and its clinical significance. Methods：Resection samples and related corresponding tissues of colorectal mucosa above 5 cm of 110 colorectal cancer patients were collected. The expression of KIF4A in colorectal tissues and matched adjacent noncancerous tissues was detected by immunohistochemical. The relationship of KIF4A expression with clinicopathological characteristics and follow-up data were statistically analyzed. Results：The expression of KIF4A in colorectal tissues was significantly higher than that in normal tissues（P < 0.001）. The expression of KIF4A was associated with degree of tumor differentiation（P=0.023），lymph node metastasis（P=0.020），distant metastasis（P=0.032），TNM stage（P < 0.001）and serum CEA（P=0.014），but not with gender，age，tumor size（all P > 0.05）. Among the 110 patients with colorectal cancer who were followed，Kaplan-Meier survival curves showed that five year’s survival rate of the high KIF4A expression group was lower than that of the low KIF4A expression group. Univariate survival analysis showed that the expression of KIF4A，degree of tumor differentiation，lymph node metastasis，distant metastasis，TNM stage and serum CEA were associated with the survival time after surgical resection. Multivariate COX proportional hazard regression model analysis further revealed that the expression of KIF4A，TNM stage，lymph node metastasis and distant metastasis were the independent risk factors for prognosis. Conclusion：KIF4A is highly expressed in colorectal cancer tissues，up-regulated expression of KIF4A is associated with metastasis and invasion of human colorectal cancers，which indicates that KIF4A may be valuable in predicting prognosis and could be a potential target for therapy of colorectal cancer.

Abstract:Objective：To explore the efficacy and safety of dexmedetomidine in sedation and analgesia during implantation of intrathecal drug infusion system（IDDS）for patients with advanced cancer pain. Methods：A total of 40 patients with advanced cancer pain classified as ASAⅡ~Ⅲ scheduled for IDDS implantation surgery were randomly assigned to two groups：the dexmedetomidine combined with fentanyl group（the Dex group，n=20）and the single fentanyl group（the Fen group，n=20）. In the Dex group，patients were infused with dexmedetomidine at a loading dose of 0.5 μg/kg within 10 min before operation，followed by a maintenance infusion of 0.2-0.5 μg/（kg·h）until the end of surgery. Patients in the Fen group were infused with equal volume of normal saline. All patients received 1 μg/kg fentanyl intravenously 5 minutes before operation. During the operation，fentanyl was intermittently injected according to patients’ visual analogue score（VAS）scores. MAP，HR，SpO2，VAS and Ramsay scores were measured and recorded for this study at T0（after entering operation room），T1（the beginning of operation），T2-T7（operation for 15 min，30 min，45 min，60 min，75 min and 90 min），T8（the end of operation），T9（15 min after arrival at PACU）and T10（30 min after arrival at PACU）. The incidences of side effects（nausea，vomiting，respiratory depression，etc.），fentanyl consumption，time to full recovery and patient satisfaction scores were also recorded. Results：Compared with the Fen group，the MAP was significantly lower in the Dex group at T2 and T3（P < 0.05）. In the Dex group，HR was significantly lower at T2-T8，whereas SpO2 was higher at the same time points mentioned above（P < 0.05）. VAS scores were significantly lower in the Dex group compared with the Fen group at T3-T5 and T7-T10，whereas Ramsay scores were significantly higher at T1-T10（P < 0.05）. Compared with the Fen group，patients required less fentanyl consumption and acquired higher satisfaction scores in the Dex group（P < 0.05）. Conclusion：Dexmedetomidine combined with fentanyl can be safely used in patients with advanced cancer pain for IDDS implantation surgery，which can achieve better sedation and analgesic effect，less fentanyl consumption and higher satisfaction scores.

Abstract:Objective：To investigate the clinical features of chronic hepatitis B patients after stopping nucleos（t）ide analogues and related factors for hepatitis B relapse. Methods：We investigated 73 chronic hepatitis B patients who withdrew nucleos（t）ide analogues and analyzed the reasons for withdrawal and related factors for hepatitis B relapse. Results：Those who stopped lamivudine had longer relapse time compared with combination therapy（P=0.031）. Among patients with total treatment duration more than 24 months，those who stopped entecavir had longer relapse time compared with combination therapy（P=0.048），and lower HBV DNA level while relapsed compared with lamivudine（P=0.039）. The COX proportional hazards model analysis showed that total treatment duration was the risk factor for hepatitis B relapse after stopping nucleos（t）ide analogues with patients who did not achieve cessation criteria. Conclusion：Most patients stopped nucleos（t）ide analogues without achieving cessation criteria. There was a still high relapse rate among patients in spite of they had achieved cessation criteria. The longer antiviral treatment duration was associated with a short time recurrence with those who did not achieve cessation criteria.

Abstract:Objective：To introduce the application of the wireless remote temperature monitoring system in phase Ⅰ clinical trial of the new recombinant adenovirus vector Ebola vaccine，compare its sensitivity with the traditional mercury thermometer measurement，and discuss the vaccine immunization fever factors. Methods：This randomized，double-blind，placebo-controlled phase Ⅰ trial of 120 healthy subjects aged 18 to 60 years who met the inclusion criteria of Taizhou，Jiangsu Province，was conducted. Body temperature was monitored within 6 hours after the Ebola vaccination using a wireless remote temperature monitoring system while body temperature was measured daily using a conventional mercury thermometer. Results：The wireless remote temperature monitoring system continuously measured and recorded the incidence of total fever and the incidence of primary fever within 6 hours after inoculation than mercury thermometer，and the difference was statistically significant（P < 0.05）. The study of influencing factors showed that high body mass index（BMI）was the protective factor of fever（OR=0.752，P=0.001）. The high pre-existing antibody of pre-adenovirus type 5 was the risk factor of fever（OR=2.472，P=0.043）. Conclusion：This study，for the first time，applied the wireless remote temperature monitoring system to the phase Ⅰ clinical trial of the novel recombinant adenovirus type-5 vector-based Ebola vaccine，which reduced the time interval of body temperature monitoring and found the abnormal changes of body temperature in time. To explore the factors that affect fever is conducive to the researchers to correctly explain the occurrence of fever.

Abstract:Objective：To establish a LC-MS/MS method for the determination of faropenem in human urine，and to investigate its urinary excretion after intravenous administration of faropenem in healthy volunteers. Methods：The hydrochlorothiazide was using as the internal standard. Chromatographic separation was carried on a Waters Cortecs C18（2.1 mm × 50 mm，2.7 μm）. The mobile phase was consisted of acetonitrile and 0.5% formic acid（10∶90）. Electrospray ionization source was applied and operated in the negativeion mode using multiple reaction monitoring. Urinary excretion parameters were calculated after single and multiple intravenous administration of faropenem at 300 mg and 400 mg to healthy volunteers. Results：The calibration curve was linear in the range of 0.506-506.000 μg/mL. The accuracy，inter- and intra- precision were all below 15%. The matrix effect did not affect the determination results. Analytes were stable during the study. The average cumulative excretion and average cumulative excretion rates of faropenem in healthy volunteers after single intravenous infusion of 300 mg were（65.16 ± 43.63）mg and（21.7 ± 14.5）%，respectively. Conclusion：This method is suitable for the determination of faropenem in human urine.

Abstract:Objective：To explore the differences between decompression and curettage on the treatment of mandibular odontogenic cyst. Methods：A total of 28 patients with mandibular odontogenic cyst over 3 teeth involvement in diameter（D≥6 cm）were selected and divided into the decompression group and the curettage group. The operation time and postoperative recovery were compared between the two groups. The preoperative and postoperative cone-beam CT（CBCT）data after 3，6，9 and 12 months were collected and reconstructed in three-dimension. The changes of bone volume fraction of the lesion and the newly regenerated bone were measured，while the relative position between cyst and the neural tube was also observed. Results：Mean operation time of the decompression group was significantly shorter than that of the curettage group（P < 0.05）. The class-A healing rate of the decompression group was significantly higher than that of the curettage group（P < 0.05）. The average cyst volume decreased more in the decompression group than in the curettage group in 3，6，9 and 12 months after the operation（P < 0.05）. The bone volume fraction of newly regenerated bone was significantly higher in the curettage group than the decompression group after 3 and 6 months（P < 0.05）. However，there was no significant difference after 9 months（P > 0.05）. It was significantly lower in the curettage group after 12 months（P < 0.05）. Ten cases with neurothlipsis preoperatively all had a recovery of neural tube in CT images after the decompression. Conclusion：Decompression can be a better choice for the treatment of mandibular odontogenic cyst.

Abstract:Objective：To introduce the technique of reduction and internal fixation of zygomatic maxillary fracture with three-dimensional navigation technique and evaluate its therapeutic effect. Methods：A total of 10 patients with zygomatic maxillary fracture of the upper jaw were selected. Cone-beam CT（CBCT） were taken before the operation and data were imported into AccuNavi software. The mirror reduction principle，preoperative planning of fracture reduction and real-time navigation technique were used to accurately guide the reduction of the fractures at the end of the operation. CBCT was taken one month after surgery and the difference between the reference points and the sagittal and coronal planes was evaluated before and after surgery. Results：The open reduction and internal fixation of fracture reduction of all the patients were successfully performed by the three-dimensional reconstruction of surgical navigation technology. Preoperative CT data analysis showed that the D-value range between the affected side and uninjured side of 0.037-9.257 mm，and the postoperative CT data analysis showed that the D-value range of 0.000-3.490 mm. The postoperative difference was significantly lower（P＜0.001），suggesting good postoperative reduction. The dimensions of preoperative health ipsilateral 2-4 mm value ratio of 33.9%（61/180），4-6 mm ratio was 7.8%（14/180），>6 mm ratio was 6.1%（11/180）；after the dimensions of health ipsilateral difference was <4 mm，and the ratio of 2-4 mm was 3.3%（6/180）. Conclusion：Three-dimensional image processing combined with computer aided navigation can be used to guide the accurate reduction of zygomatic fracture and evaluate the postoperative aesthetic effect.

Abstract:Objective：To explore and compare the relationship between the different parts of abdominal fat as determined by ultrasonic measurements and the cardiovascular risk factors. Methods：A total of 130 males from the authors’ affiliated hospital were involved，anthropometric and metabolic parameters were measured. Each subject underwent ultrasonography to estimate the visceral fat thickness in the mid-abdominal cavity（VFT-M），the visceral fat thickness in the upper abdominal cavity（VFT-U）and mesenteric fat thickness（MFT）. Results：All of the abdominal fat thickness were positively correlated to body mass index（BMI），waist circumference（WC），hip circumference（HC），waist-hip ratio（WHR），fasting insulin（FINS），triglyceride（TG），and negatively correlated to age，high-density lipoprotein（HDL-C），the VFT-U and MFT were positively correlated to insulin resistance index（HOMA-RI），the MFT was positively correlated to uric acid（UA）. The partial correlation analysis after adjusting for age，BMI and WC showed that：VFT-U was positively associated with HOMA-IR and TG；MFT was positively associated with FINS，HOMA-IR and was negatively associated with HDL-C；VFT-M showed no association with neither cardiovascular risk factors. The stepwise multiple linear regression showed that：VFT-U and MFT，especially the MFT，were more valuable for the prediction of cardiovascular risk factors. Conclusion：VFT-U and MFT are good correlated with cardiovascular risk factors compared with VFT-M. VFT-U and MFT are associated with different cardiovascular risk factors. Different parts of abdominal fat recognized and measured by ultrasonography show a great clinical significance.

Abstract:Objective：To identify the risk factors for early mortality（≤30 days）in patients with ruptured naive hepatocellular carcinoma（HCC），including treatment modality. Methods：Between Jun 2012 and Dec 2016，the medical records of 80 ruptured naive HCC patients who received conservative treatment or transarterial embolization（TAE）as initial therapy from the authors’ affiliated hospital were retrospectively analyzed. According to the survival state within 30 days，the selected patients were divided into the survival group and the dead group. An independent sample t-test was used for the continuity variables between the two groups，and the constituent ratios and rates were tested using the χ2 test. The possible risk factors related to early mortality were analyzed by using univariate and multivariate logistic regression model（P < 0.05）. Results：Of 80 patients，the early mortality was 28.8%. Univariate analysis showed that the proportion of patients with shock on admission（P=0.008），conservative treatment（P < 0.001），poor Child-Pugh class（P < 0.001）and advanced modified tumor stage of the liver cancer study group of Japan（LCSGJ）（P < 0.001）in the dead group was significantly higher than those in the survival group，and the patients’ initial hemoglobin level of the dead group（P < 0.001）was also significantly lower than that of the survival group. Multivariate Logistic regression analysis indicated that patients with shock on admission（OR=5.543，P=0.026），poor Child-Pugh class［class B（OR=10.395，P=0.009）and class C（OR=23.633，P=0.006）］and conservative treatment（OR=8.576，P=0.002）were independent risk factors for early mortality. Conclusion：Among ruptured naive HCC patients，the early mortality is high. For such patients，actively correcting shock while improving liver function as early as possible should undoubtedly have great significance for improving the prognosis. Additionally，reasonably expanding the indications of interventional therapy is essential.