Abstract:Objective：To investigate the effect of down-regulating PTEN on cell cycle in HeLa cells and to explore its underlying mechanism. Methods：PTEN-specific short hairpin RNA（shRNA）was synthesized and introduced into HeLa cells via lentivirus. The mRNA and protein expression of PTEN and Cyclin B1 were detected by qPCR and Western blot assay，respectively. Cell cycle distribution was determined by flow cytometry. Cyclin B1 protein expression was examined by Western blot after proteasome inhibitor MG-132 treatment to evaluate its protein stability. Results：Compared to the control group，both mRNA and protein levels of PTEN were decreased in HeLa cells transfected with PTEN-specific shRNA. PTEN down-regulation in HeLa cells induced cell cycle arrest in G2/M phase，decreased Cyclin B1 protein level，but had no effect on Cyclin B1 mRNA level. After MG-132 treatment，the expression of Cyclin B1 protein in the PTEN interference group was significantly elevated. Conclusion：Inhibition of PTEN expression in HeLa cells led to cell cycle arrest in G2/M phase，which could be caused by decreased expression level of cell cycle related proteins（Cyclin B1）.

Abstract:Objective：To explore the influence on expression of growth differentiation factor 5（GDF-5）in adipose-derived stem cells（ADSCs）by inhibiting the expression of Noggin. Methods：The recombinant lentivirus vectors of GDF5 gene（Lv-GDF5）and lentivirus vectors carrying Noggin RNAi sequence（Lv-Noggin shRNA）were constructed. ADSCs were divided into four groups：ADSCs in group A were transfected by empty lentivirus（with enhanced green fluorescent protein，Lv-EGFP）；ADSCs in group B were transfected by Lv-Noggin shRNA；ADSCs in group C were transfected by Lv-GDF5；ADSCs in group D were co-transfected by Lv-GDF5 and Lv-Noggin shRNA. Then，the expression of GDF5 and Noggin in ADSCs was determined by real-time PCR and Western blot 14 days after transfection. The capacity of cartilage differentiation was detected through collagen typeⅡ（Col-Ⅱ）by RT-PCR. Finally，the expression quantity was contrastly analyzed. Results：RT-PCR showed that the expression of GDF5 mRNA and protein increased gradually 14 d after transfection from group A to group D. In comparison between each two groups，except the difference between group B and group C was not statistically significant，differences between the other groups were statistically significant（P < 0.05）. The expression of Noggin mRNA and protein increased gradually in group D，B，C and A. In comparison between each two groups，except the difference between group A and group C was not statistically significant，the differences between the other groups were statistically significant（P < 0.05）. The expression of Col-Ⅱ mRNA increased gradually from group A to group D. In comparison between each two groups，the differences were statistically significant（P < 0.05）. The results of Western blot showed that the expression of GDF5 protein increased gradually from group A to group D 14 d after transfection. In comparison between each two groups，the difference was statistically significant（P < 0.05）. The expression of Noggin protein decreased gradually in group A，C，B and D. In comparison between each two groups，the differences between group A and group C，as well as group C and group B were not statistically significant，while the differences between the other groups were statistically significant（P < 0.05）. Conclusion：The expression of GDF5 in ADSCs increased by inhibiting the expression of Noggin. A negative correlation between GDF5 and Noggin expression quantity was found.

Abstract:Objective：To explore the effects of Tomoregulin-1 on dilated cardiomyopathy（DCM）pathological progress in mice. Methods：The expression level of Tomoregulin-1 in the heart of wild-type and cTnTR141W DCM transgenic mice was detected by Western blot. The phenotype analysis of the non-transgenic littermates（NTG），heart-specific Tomoregulin-1 knockdown（Tomoregulin-1-kd），cTnTR141W and heart-specific Tomoregulin-1-kd×cTnTR141W double transgenic（DTG） mice at 2，4 and 6 months of age were analyzed by M-mode echocardiography and histopathologic examination. Results：The expression of Tomoregulin-1 was significantly increased in the heart of cTnTR141W DCM transgenic mice. M-mode echocardiography showed that the heart-specific Tomoregulin-1-kd mice presented thin-walled ventricles，larger left ventricular diameters and decreased cardiac function at 2，4 and 6 months of age compared with the NTG mice. The DTG mice presented significantly thin-wall ventricles and the tendency of larger left ventricular diameters and decreased cardiac function at 2，4 and 6 months of age compared with the cTnTR141W DCM mice. Myocardial disarray and fibrosis were clearly observed in the heart tissues from the Tomoregulin-1-kd mice compared with the NTG mice. The DTG mice presented more serious pathological phenotype compared with the cTnTR141W DCM mice. Conclusion：Transgenic low expression of Tomoregulin-1 accelerated DCM pathological progress in mice. Tomoregulin-1 may be an important modifier gene of DCM.

Abstract:Objective：The present study focuses on the effect of salubrinal on energy intake，body weight，blood glucose，serum insulin and morphological changes of pancreatic islets in gestational diabetic mice induced by high-sucrose/high-fat diet. Methods：Female C57BL/6J mice were divided into three groups randomly：normal control group（C），gestational diabetic model group（G）and salubrinal intervention group（G+S）. Energy intake，body weight，food intake and blood glucose were measured at multiple time points before and during pregnancy. At the 10th day of pregnancy，the blood was collected for the measurement of serum insulin levels. Then the scores of pancreatic β-cell function（HOMA-B）and insulin resistance（HOMA-IR）were calculated. At the 17th day of pregnancy，the mice were sacrificed，and the pancreas was collected for morphological examination. Results：The energy intake and body weight of group G and group G+S were respectively higher than that of group C at multiple time points before and during pregnancy，whereas there was little difference in these two parameters between group G and group G+S. After 1-month feeding，the tendency of blood glucose level was showed as：group G+S > group G > group C，and this tendency was more significant at the 10th day of pregnancy. However，there was no significant difference in blood glucose among the 3 groups at the 17th day of pregnancy. Although there was little difference in serum insulin levels among the 3 groups at the 10th day of pregnancy，HOMA-B showed：group G+S < group G < group C，and HOMA-IR showed：group G+S > group G > group C. Morphological examination showed that the morphology of pancreatic islets and the ultrastructure of pancreatic β cells in group C were normal. However，the number of pancreatic islets increased in group G and group G+S. The hyperplasia and atrophy of pancreatic islets occurred simultaneously in these 2 groups. Meanwhile，the abnormal ultrastructure of pancreatic β cells was characterized by severe swollen mitochondria and highly expansion of rough endoplasmic reticulum. Conclusion：These findings suggest that salubrinal has no potential for preventing and controlling gestational diabetic mice induced by high-sucrose/high-fat diet.

Abstract:Objective：To investigate the effect of stanniocalcin（STC1） on epithelial-mesenchymal transition（EMT） in chronic obstructive pulmonary disease（COPD）and the expression in bronchial epithelial cells. Methods：Western blot and immunofluorescence were used to examine EMT associated biomarkers including E-cadherin and alpha smooth muscle actin（α-SMA）. The STC1 mRNA and protein levels stimulated by cigarette smoke extract（CSE） in human bronchial epithelial（16HBE）cells were analyzed by real-time polymerase chain reaction（RT-PCR）and Western blot. Results：TGF-β induced EMT of 16HBE cells where the cell morphology changed from a typical multilateral paving stone-like appearance to a mesenchymal-like fusiform appearance along with the decreased expression of epithelium biomarker E-cadherin and the increased expression of mesenchymal cell markers α-SMA. rhSTC1 significantly inhibited the EMT changes mentioned above. The expression of STC1 was increased by CSE in a concentration-dependent manner in 16HBE cells. And this effect was inhibited by NF-κB inhibitor JSH23. Conclusion：STC1 was a protective factor to inhibit EMT during the development of COPD. CSE may stimulate bronchial epithelial cells，and increase the expression of STC1 through NF-κB signal，which may be the main source.

Abstract:Objective：To observe the effects of mitochondrion-targeted antioxidant SS-31 on hippocampal mitochondrial morphology and cognition after isoflurane-anesthesia in developing rats. Methods：Seventy-two postnatal day（PND）7 rats were equally divided into 3 groups（n=24）：oxygen + normal saline（the CN group），isoflurane + normal saline（the IN group），and isoflurane + SS-31（the IS group）. SS-31（5 mg/kg）or normal saline was intraperitoneally administered with a volume of 0.4 mL/kg 30 min before gas inhalation. Malondialdehyde（MDA）content，reactive oxygen species（ROS）levels，and superoxide dismutase（SOD）activities were determined on PND7. Mitochondrial morphology was determined on PND21，and behavioral studies were performed on PND40 or PND60. Results：Compared with the IN group，the levels of ROS and MDA，and number of abnormal mitochondria were decreased，while the activity of SOD，times of crossing-platform and time spending in target quadrant were increased in the IS group. Conclusion：Mitochondrial antioxidant SS-31 attenuates isoflurane-induced oxidative stress，and impairments of mitochondrial morphology and cognition in developing rats.

Abstract:Objective：To investigate the role of Bmi-1 in preventing atrophic gastritis in mice. Methods：The phenotype of stomach was compared between wild-type and Bmi-1 gene knock-out mice at 7 weeks of ages by histology，immunohistochemistry and Western-blot. Results：Bmi-1 gene knock-out mice displayed thin gastric wall，inflammatory infiltration in gastric mucosa and atrophy of gastric gland. The expression of interleukin-6（IL-6），tumor necrosis factor α（TNF-α） and nuclear factor-κB-p65（NF-κB-p65） is up-regulated in Bmi-1 knock-out mice. Conclusion：Bmi-1 could protect mice from atrophic gastritis by down-regulating NF-κB signaling pathway.

Abstract:Objective：This study was designed to examine the interaction of cefminox sodium（CMNS）with bovine serum albumin（BSA）under the optimum conditions. Methods：The mechanism of the interaction between CMNS and BSA was studied by spectroscopic techniques combination with absorption spectroscopy. Results：Stern-Volmer analysis of fluorescence quenching data showed the presence of the static quenching mechanism. The thermodynamic parameters indicated that the electrostatic interactions were the predominant intermolecular forces stabilizing the complex. The number of binding sites（n）and binding constant（Kb）was calculated. The binding process was spontaneous. The obtained data for binding sites of n approximately equal to 1 indicated that there was a single class of binding site for the BSA with CMNS. The primary binding site for CMNS was located at sub-domain ⅡA of BSA and nearby tyrosine residue. The conjugation reaction would affect the conformation of BSA，leading to the polarity around BSA strengthened. Conclusion：There was almost some negative cooperative effect between CMNS and BSA. The obtained results provided references for its clinical application.

Abstract:Objective：The study aimed to investigate differentially expressed microRNAs（miRNAs）and their association with left ventricular end-diastolic dimension（LVEDd），left ventricular ejection fraction（LVEF），N-terminal of the prohormone brain natriuretic peptide（NT-proBNP）in human dilated cardiomyopathy（DCM）by miRNAs array，and to predict their target gene. Methods：The expression levels of plasma miRNAs of 8 DCM patients and 4 healthy controls were detected by using the Agilent human miRNAs array（V19.0），followed by real-time RT-PCR analysis to validate the expression changes of miRNAs. NT-proBNP，LVEDd and LVEF were measured and analyzed using Pearson linear correlation analysis. The prediction analysis for microarrays（PAM）method was used to identify the differentially expressed miRNAs. Results：Thirty-six differentially expressed miRNAs were identified. There were 25 upregulated and 11 downregulated human miRNAs，of which miR-5196-5p and miR-652-5p were the most significant. Pearson linear correlation analysis showed that miR-5196-5p level was positively correlated with LVEDd，whereas miR-652-5p was positively correlated with LVEF values and negatively correlated with NT-proBNP and LVEDd. Moreover，correlative genes such as VCL and RBM20 related with DCM were predicted. Conclusion：The screened differentially expressed miRNAs may be involved in the development of DCM. Specific miRNAs，such as miR-5196-5p and miR-652-5p，may be considered as new targets for the diagnosis and treatment of human DCM.

Abstract:Objective：To explore the effects of temozolomide combined with postoperative treatment on serum miR-181b and miR-497 levels in patients with glioma. Methods：Seventy-six patients with postoperative glioma were randomly divided into two groups，namely，group A（n=38）and group B（n=38）. Patients in group A were treated with semustine and those in group B were treated with semustine combined with temozolomide. After the treatment，the effective rate，1-year，2-year and 3-year survival rates，Karnofsky scores in the two groups were compared. The serum samples of patients in B group were collected to detect the changes of miR-181b and miR-497 expression before and after treatment. Results：Objective response rate and disease control rate in group B were significantly higher than those in group A（P < 0.05）. The median survival time in group A was shorter than that in group B. And 1-year，2-year and 3-year survival rates after the operation in group A were significantly lower than those in group B（P < 0.05）. Karnofsky scores after the treatment was significantly lower in group A than that in group B（P < 0.05）. The serum levels of miR-181b were significantly increased after operation［postoperative（162.34 ± 51.79）fmol/L vs. preoperative（91.37 ± 40.41）fmol/L，P < 0.05］. The serum levels of miR-497 were significantly increased after operation［postoperative（166.23 ± 53.68）fmol/L vs. preoperative（88.36 ± 36.72）fmol/L，P < 0.05］. Conclusion：Temozolomide combined with postoperative treatment can improve the expression of miR-181b and miR-497 in serum of patients with glioma，and miR-181b and miR-497 can be used for the diagnosis and prognosis of glioma.

Abstract:Objective：The goal of this study was to compare the signal peptide-CUB（complement C1r/C1s，Uegf，and Bmp1）-EGF（epidermal growth factor）domain-containing protein 1（SCUBE1） levels between acute pulmonary thromboembolism（APTE）patients and healthy subjects and also investigate the value of SCUBE1 combined D-dimer in the diagnosis of APTE. Methods：Thirty-one patients diagnosed with APTE using computed tomographic pulmonary angiography（CTPA）were included in the study as the experimental group. A control group of 19 age-matched，healthy volunteers served as a reference for biochemical parameters. The levels of SCUBE1 from serum in the experimental and control groups were measured，and the Mann-Whitney rank plasma test was used to compare the levels of SCUBE1 in the two groups. The receiver operating curve（ROC）was used to analyze and compare the diagnostic value of SCUBE1，D-dimer for APTE and the combined diagnostic value of both. Results：SCUBE1 levels were significantly higher in the patients with APTE（P＜0.001）. The best cut-off value of SCUBE1 in the diagnosis of APTE was 8.09 ng/mL. The sensitivity of diagnosis of APTE was 87.10% and the specificity was 68.42%. The sensitivity of SCUBE1 combined with D-dimer to diagnose APTE was 81.48%，and the specificity was 88.37%. Conclusion：SCUBE1 combined D-dimer assist in the diagnosis of APTE.

Abstract:Objective：To study the risk factors of pulmonary adenocarcinoma with venous thromboembolism（VTE），and to improve the understanding of lung adenocarcinoma with VTE. Methods：The clinical data of 2 930 patients with lung adenocarcinoma admitted to the First Affiliated Hospital of NMU from January 2012 to December 2016 were retrospectively analyzed，and 96 cases were found to have VTE. Patients with VTE were included in the VTE group. Patients without VTE were included in the non-VTE group. The risk factors of VTE in lung adenocarcinoma were analyzed. Results：Among the 2 930 cases of lung adenocarcinoma，96 cases/2 930 cases（3.3%）were associated with VTE. In the VTE group，there were 57 males and 39 females，with an average age of （60.8 ± 10.1）years. In the non-VTE group，there were 1 477 males and 1 357 females，the average age was（61.0 ± 10.5）years old. There were signicant differences in smoking，central venous catheterization，chronic pulmonary diseases and stage of lung adenocarcinoma between two groups（P < 0.05）. Multiple factor analysis suggested that the chronic pulmonary disease and Ⅲ~Ⅳ stage of lung adenocarcinoma were high risk factors for lung adenocarcinoma associated with VTE. Of the 96 patients with VTE group，26 cases（27.1%）occurred in pulmonary thromboembolism（PTE）only，51 cases（53.1%）occurred in deep vein thrombosis（DVT）only，and 19 cases（19.8%）occurred in PTE and DVT. Among 45 patients with PTE，37 cases（82.2%）showed obvious clinical symptoms，and 8 cases（17.8%）had no obvious symptoms. Among 70 patients with DVT，66 cases（94.3%）showed swelling or pain on the affected side. Seventy-one patients in VTE group were accompanied by VTE after treatment of lung cancer. For 0-3 months，3~6 month，6-9 months，9-12 months and >12 months，the number of VTE cases was 37，8，8，4 and 14，respectively. At the end of follow-up，52 cases/96 cases（54.17%）died，and the median survival time of patients with VTE was 5 months. Conclusion：There was a significant correlation between Ⅲ-Ⅳ stage of lung adenocarcinoma，chronic pulmonary disease and lung adenocarcinoma with VTE. VTE was the most likely to occur in the first 3 months of the diagnosis of lung adenocarcinoma.

Abstract:Objective：To explore the association of urine acidification function in patients with diabetic kidney disease（DKD）. Methods：A total of 42 DKD patients diagnosed by renal biopsy presenting to the first affiliated hospital of NMU from January 2015 to March 2017 were recruited. The prevalence of different kinds of renal tubular injury was analyzed by acidification function. In addition，urine acidification function of different stages，the correlation of urine pH levels and clinical parameters were compared. Results：Patients with DKD often had dysfunction in acidification of the tubules，of which the most common was dysfunction of the distal tubules in the secretion of ammonium，followed by dysfunction of the distal tubules in the secretion of hydrogen，and the least common was the reabsorption of bicarbonate dysfunction in the proximal tubules. The prevalence of the distal tubules dysfunction in the secretion of ammonium showed an increasing trend in different pathological stages（P=0.007）. Patients with re-absorption of bicarbonate dysfunction in DKD patients had higher parathormone levels（P < 0.05）. Differences in 24-hour urinary protein and hemoglobin levels between patients with and without distal tubules of hydrogenation dysfunction were observed（P < 0.05）. The levels of hemoglobin were significantly different between patients with or without ammonium secretion dysfunction in the distal tubules（P < 0.05）. Fasting urine pH was positively correlated with 24-h urinary protein level and negatively correlated with serum albumin. Especially when the urinary protein was higher than 3.5 g/d，there was a good linear correlation between urinary protein quantification and urine pH. Conclusion：The most common dysfunction of tubular acidification in patients with DKD was ammonium secretion in the distal tubules. Patients with titer acid anemia had lower hemoglobin levels and higher urinary protein levels than patients in the titer acid normal group. The increase of fasting urine pH in DKD patients may be related to 24-h urinary protein.

Abstract:Objective：The aim of this retrospective study was to investigate serum magnesium level in peritoneal dialysis（PD）patients in a single PD center and to compare the differences of demographics，dialysis status，and biochemical parameters among PD patients with different magnesium levels. Methods：This study analyzed the demographic characteristics as well as clinical and laboratory measurements of 147 patients in our dialysis center. These patients were divided into three groups according to serum magnesium concentration：group A：Mg2+<0.77 mmol/L，group B：0.77 mmol/L≤ Mg2+≤1.03 mmol/L，group C：Mg2+>1.03 mmo/L. Results：For serum phosphorus，serum calcium，serum potassium，serum urea nitrogen of dialysis patients：group A was significantly lower than group C；serum albumin：group B was significantly lower than that in group C；serum creatinine was significantly lower in group A than in group B and group C；for urea clearance index（Kt/V） and the standardized creatinine clearance rate：group B and group C was significantly lower than group A；The bivariate correlation analysis showed that serum magnesium was positively associated with serum phosphorus，and intact parathyroid hormone. Serum magnesium was also negatively associated with Kt/V and the standardized creatinine clearance rate. Moreover，our study found that the levels of serum magnesium were different in patients with different peritoneal transport characteristics which was divided by peritoneal equilibration test（P < 0.01）. Conclusion：There was an abnormal metabolism of magnesium in patients with PD. Among them，hypermagnesemia was more frequent than hypomagnesemia. Patients with lower serum magnesium concentration and high peritoneal transport capacity had better dialysis adequacy. Moreover，abnormal serum magnesium levels were often associated with other electrolyte and metabolic disorders. The levels of serum calcium，phosphorus and potassium may be higher and the serum glucose level may be lower in patients with hypermagnesemia.

Abstract:Objective：To investigate the roles of visceral adiposity index （VAI） and Chinese visceral adiposity index （CVAI） in assessments of insulin resistance and abnormal glucose metabolism. Methods：Community-dwelling individuals aged over 40 years in the REACTION research were enrolled in our study. Demographic characteristics，disease history and physical examination information were collected by trained technicians. Blood glucose，lipid profile and insulin concentrations were examined. Results：A total of 7 523 individuals were enrolled in our study，the average age of subjects is 54.4 years. Both VAI and CVAI had a positive relationship with homeostasis model assessment of insulin resistance（HOMA-IR）（r=0.277，P < 0.001；r=0.322，P < 0.001）and were negatively correlated with Matsuda insulin sensitivity index（ISI）（r=-0.400，P < 0.001；r=-0.432，P < 0.001）. Compared to those in lowest quartile，subjects in uppermost quartile of VAI had the strongest association with insulin resistance and diabetes（top vs. bottom，OR：3.309，3.669），it was the similar with CVAI（top vs. bottom，OR：5.095，5.434）. Conclusion：VAI and CVAI may be simple and practical clinical indicators for insulin resistance and glucose metabolism evaluation in middle-aged and the elderly Chinese.

Abstract:Objective：To explore the correlation of serum chemerin and the ratio of non-high-density lipoprotein cholesterol（non-HDL-C）to high-density lipoprotein cholesterol（HDL-C） with microalbuminuria for patients with type 2 diabetes（T2DM）. Methods：According to urinary albumin to creatinine ratio（UACR），254 T2DM patients were divided into the normal albuminuria group（the NAU group，UACR<30 μg/mg，129 cases）and the microalbuminuria group（the MAU group，30 μg/mg

Abstract:Objective：To investigate the expression and clinical significance of interleukin（IL）-36γ in serum and bronchial epithelial cells of asthma patients. Methods：The levels of serum IL-36γ of 24 cases of asthma patients and 24 cases of healthy volunteers were determined by enzyme-linked immunosorbent assay（ELISA）. The effects of asthma-related inflammatory mediators on IL-36γ expression were examined by real time quantitative PCR in human bronchial epithelial cell line（16HBE）. Results：The levels of serum IL-36γ were significantly increased in asthma patients compared with healthy controls［（175.90 ±25.70）pg/mL vs.（100.40 ± 8.49）pg/mL，P=0.008］. Specifically，The increased levels of serum IL-36γ were negatively correlated with the reduced lung function including FEV1/FVC（r=-0.347，P=0.016）and FEV1% pred（r=-0.454，P=0.001）and had a positive correlation with serum IL-13 levels（r=0.611，P=0.003）. Moreover，IL-36γ expression was highest in uncontrolled group of asthma patients than those without asthma（P < 0.001）or those with well to partly controlled asthma（P < 0.05）. In vitro，IL-13 treatment induced a dose and time-dependent up-regulation of IL-36γ mRNA expression in 16HBE. Conclusion：Serum IL-36γ levels are elevated in asthma patients compared with healthy control subjects. Serum IL-36γ may be a potential novel circulating biomarker for the disease assessment and monitoring of asthma.

Abstract:Objective：To evaluate the efficiency and safety of haploidentical hematopoietic stem cell transplantation（haplo-HSCT）for treatment of secondary myelodysplastic syndrome（sMDS） after AA. Methods：Evaluation of therapeutic effect，incidence rate of aGVHD and cGVHD，complications and overall survival（OS） of 5 AA post MDS patients who received haplo-HSCT in this center，and compared with other treatments for sMDS evolved from AA. Results：Among 5 patients，median OS was 63 months（range from 41.9 to 149.3 months），OS after HSCT was 12.9 months（range from 2.4 to 36.5 months）. 1-year event free survival was 60%. All patients achieved neutrophil and platelet engraftment at a median of 18 days（range from 14 to 22 days）and 21 days（range from 15 to 65 days）respectively. 3 occurred Ⅰ~Ⅲ° acute graft versus host disease（aGVHD），and 1 with chronic graft versus host disease（cGVHD）. Medial follow-up was 63 months（range from 41.9 to 149.3 months）. Conclusion：The haplo-HSCT can be an opition for sMDS from AA patients in case of an HLA-identical donor is not available.

Abstract:Objective：To compare dosimetric difference between the intensity modulated radiotherapy（IMRT）and volumetric modulated arc therapy（VMAT）of cervical cancer with para-aortic lymph node metastasis，and to provide the reference basis for selecting the reasonable radiotherapy approach of locally advanced cervical cancer patients. Methods：Twenty patients of cervical cancer with para-aortic lymph node（PALN）metastasis whom confirmed by PET-CT examination and diagnosis and received radiotherapy were selected，and the same CT image was designed by IMRT and VMAT respectively. Then，we compared the parameters of target，organ at risk（OAR），homogeneity index（HI），conformity index（CI），the number of monitor units（MU）and treatment times between two plans. Results：The target dose of IMRT plan and VMAT plan could meet the dosimetric requirement. The CI and HI of planning target volume（PTV） for VMAT plan were superior to IMRT plan（P＜0.05）. Compared with the IMRT plan，the mean dose of kidney for VMAT plan were lower（P＜0.05）. The V40 and V50 of the rectum，V40 of the small intestine and the bladder of VMAT plan were better than those of IMRT plan（P < 0.05）. The number of MU for VMAT plan（859.92 ± 248.47）were lower than IMRT plan（1 649.50 ± 167.44）（t=11.836，P＜0.001）. The treatment time for VMAT plan［（304.30 ± 41.98）s］were shorter than IMRT plan［（435.90 ± 37.52）s，t=12.750，P＜0.001］. Conclusion：Both of the IMRT and VMAT plans can achieve the clinical dosimetric demands and protect the OARs of cervical cancer with PALN. VMAT has the best performance on CI and HI，and protect the OARs better. VMAT plans have fewer MU and significantly improve the treatment efficiency. Thus it will improve the patient's radiotherapy tolerance，enhance the efficiency of radiotherapy.

Abstract:Objective：To evaluate the application of functional MRI in renal transplantation by comparing the parameters of diffusion weighted imaging（DWI） and diffusion tensor imaging（DTI） between patients who suffered from delayed graft function（DGF）after transplantation and patients who not，and by analyzing the correlation between parameters of functional MRI and renal allograft function. Methods：Patients who underwent kidney transplant in our center from September 2015 to May 2017 were retrospectively reviewed，and 23 DGF patients and 26 normal graft function controls were enrolled in the study. Comparisons of apparent diffusion coefficient（ADC）and fractional anisotropy（FA） values of renal allograft were both made between DGF patients and controls one month after transplant；And correlations of ADC and FA values one month after transplant were made between estimated glomerular filtration rate（eGFR）and serum creatinine value of one and six months after transplant respectively. Differences of ADC and FA value between DGF patients and controls were compared by student’s t test；Relationships between MRI parameters and eGFR and serum creatinine were analyzed respectively using Pearson correlation test. Receiver operating characteristic（ROC） curve of medullar ADC and FA value were both created for prediction of eGFR>60 mL/min. Results：Differences of cortical and medullar FA value were found between DGF group and control group（P < 0.05）. Positive correlation between medullar FA and eGFR was observed one month after transplantation，as well as medullar ADC and eGFR（P < 0.05）. A negative correlation was found between cortical ADC of one month after transplantation and serum creatinine level at 6 months’ post-transplant period（P < 0.05）. For prediction of eGFR >60 mL/min，the ROC curve of medullar ADC and FA showed the area under the curve（AUC）of 0.796 and 0.799 respectively，and 95% confidence interval（CI）of 0.670-0.922 and 0.671-0.928 respectively（P < 0.05）. Conclusions：Functional magnetic resonance DWI and DTI can provide functional and morphological assessment of transplanted kidneys.

Abstract:Objective：To investigate brain changes of cortical gray volume and function between dementia with Lewy body（DLB）and Parkinson’s disease（PD）. Methods：A total of 16 DLB patients，20 PD patients and 20 well-matched healthy controls were scanned with T1 structure and resting-state functional magnetic resonance imaging for regional homogeneity（ReHo） and gray matter volume（GMV） analyses. The Mini mental state examination（MMSE），Montreal cognitive assessment（MoCA）and unified Parkinson’s disease rating scale-Ⅲ（UPDRS-Ⅲ）were performed to assess the clinical symptom，as well as its correlations with ReHo and GMV. Results：① Compared with the controls，the DLB groups showed higher ReHo in left cerebellum anterior lobe，bilateral calcarine，bilateral caudate，and lower ReHo in left middle frontal gyrus，while PD patients had increased ReHo in right superior temporal gyrus，right supra marginal. Compared with the PD patients，the DLB patients had significantly increased ReHo in bilateral caudate，as well as decreased ReHo in right supra marginal，left inferior frontal gyrus.② Compared with the controls，the DLB patients show reduced GMV in bilateral caudate，right temporal lobe；Compared with the PD patients，the DLB patients show reduced GMV in right caudate，right temporal lobe.③ There are significantly negative correlation between ReHo and UPDRS-Ⅲ scores in bilateral caudate in DLB patients. Conclusion：The structural and functional changes in brain of DLB patients may be related to disease’s physiopathologic mechanism.

Abstract:Objective：To analyze the prevalence and epidemiological characteristics of atrial fibrillation（AF）in the elderly population in the communities of Jinshan District of Shanghai. Methods：We analyzed the data collected from the Shanghai Citizen Physical Examination Project performed in the elderly above 65-year-old in 9 communities of Jinshan District. A 12-lead electrocardiogram examination and a cardiovascular disease research questionnaire which was mainly on AF were obtained. Results：The crude prevalence rate of AF in this study population was 2.78%，standard prevalence rate was 3.02%，increased with age（P < 0.05）. The prevalence rate in males under 75 years of age was higher than that in females between each age group（P < 0.05）. The proportion of first diagnosed，paroxysmal，persistent and long-standing persistent AF was 13.72%，24.15%，7.03% and 55.10%，respectively. The average duration of AF for paroxysmal，persistent and long-standing persistent AF was （5.0 ± 5.92） years. A total of 9.61% of AF patients had a history of stroke，while only 4.92% patients received warfarin therapy. Conclusion：The AF prevalence rate and epidemiological characteristics in the elderly above 65-year-old in 9 communities of Jinshan District of Shanghai are close to other research results in China.

Abstract:Cholangiocarcinoma is a malignant tumor originating from the bile duct epithelium with a poor prognosis. At present，the specific molecular mechanism of cholangiocarcinoma is not clear，and the previous studies have focused on gene mutations in the histological level of cholangiocarcinoma. However，more and more studies have found that the development of cholangiocarcinoma is not only caused by the changes of DNA. Epigenetic regulations，such as DNA methylation，histone modification，chromatin remodeling，non-coding RNA and genetic imprinting regulation also play an important role on development of cholangiocarcinoma，which receive more attention now. We summarize the current epigenetic progress of cholangiocarcinoma，and aims to reveal the role of epigenetic regulation in the development and progression of cholangiocarcinoma.

Abstract:Influenza A virus is the main type of seasonal influenza and influenza pandemics in history. It has been widely researched at home and abroad. China has also been recognized as a new and multiple influenza virus place，which is also the outpost of world influenza surveillance. Influenza surveillance in China plays an important role in the world. The researchers use functional genomics screening and case-control study to find some genes which can affect the human influenza virus infection or aggravate the condition of patients with influenza through a variety of pathogenic mechanisms. Mutation of the host gene can affect the toxicity and the spreading of influenza A virus，which also has great effects on susceptibility to influenza. The study on susceptibility gene of influenza A，at the level of host genetic to find the pathogenesis of influenza A，in order to further explore the pathogenesis of influenza virus invasion of the host，which contributes to carry out influenza prevention and control work.