Abstract:Objective：To study the treatment effects of androgen receptor（AR）antagonist combined with class I histone deacetylase（HDAC）inhibitors on prostate tumorosphere，and to investigate the possible molecular mechanisms involved in the process. Methods：Prostate cancer LNCaP and 22RV1 cells were cultured in serum-free suspension condition，and the obtained two tumorosphere stem-like cells were treated with AR antagonist MDV3100 with or without the presence of HDAC class I inhibitor MS-275 to study the morphology change and the ability of cell clone formation in monolayer culture condition. Then，quantitative real-time fluorescence polymerase chain reaction（qRT-PCR）was utilized to analyze cancer stem cell marker CD133 expression，and Western blot was used to detect the levels of DNA damage marker H2A.X（p-H2A.X），the cleavage of poly ADP-ribose polymerase（PARP），β-catenin，proto-oncogene c-Myc and cyclin D1. Results：Treatment with MDV3100 alone inhibited CD133 expression in tumorosphere cells. Combination treatment of MDV3100 with MS-275 reduced the number of tumorosphere，inhibited CD133 transcription，enhanced the level of both PARP cleavage and p-H2A.X，decreased expression of β-catenin，c-Myc and cyclin D1. Conclusion：Compared with the treatment of MDV3100 alone，combination treatment of MDV3100 with MS-275 significantly inhibited cancer stem-like tumorosphere cell formation and promoted apoptotic cell death. The drugs-reduced over activation of Wnt/β-catenin/c-Myc/cyclin D1 pathway was possibly involved in the antitumor process. These results provide guidance for clinical application of MDV3100 and MS-275 in prostate cancer management of personal medicine.

Abstract:Objective：To determin the functions of an anti-SraPL-Lectin monoclonal antibody in the process of phagocytosis and killing Staphylococcus aureus（S.aureus）in the mouse macrophages. Methods：Differernt gene sequence of sraP was amplied by PCR and specific amplification products were inserted into pET28a plasmid. The rpET28a-SraPL-Lectin plasmid was transferred into E.coli.BL21 and induced by 0.1 mmol/L IPTG at 25 ℃. The recombinant protein was purified by nickel column and the specificity of this antibody was detected by Western blot. The expression level of inflammatory factors in macrophages was detected by qPCR. CCK8 assay was carried out to assess the inhibition rate of S.aureus proliferation. The number of S.aureus colonies in the supernatant and lysate of macrophages was counted on the coated plate. Results：Anti-SraPL-Lectin monoclonal antibody could specifically bind to recombinant SraP truncated proteins and cell wall protein of S.aureus. The co-incubation of monoclonal antibody with S.aureus could induce the down-regulation of pro-inflammatory cytokine（TNF-α，IL-1β，IL-12p40）and up-regulation of anti-inflammatory cytokine（IL-10）in macrophages. The proliferation of S.aureus USA300 LAC was obviously inhibited in the co-effect of gentamicin and antibody. Anti-SraPL-Lectin reduced the amount of S.aureus in macrophage supernatant. Conclusion：The immune complex of anti-SraPL-Lectin antibody and S.aureus can alleviate the immune response of macrophages and promote the clearance of macrophages to S.aureus.

Abstract:Objective：To establish human induced pluripotent stem cell derived atrial and ventricular cardiomyocytes for cardiotoxicity assessment. Method：Temporarily manipulating retinoic acid signal to differentiate atrial and ventricular cardiomyocytes. Flow cytometry analysis and immunofluorescent staining were conducted to detect the cardiac-specific marker：α-actinin and ventricle-specific marker：MLC2v. qRT-PCR was done to detect ventricle-specific marker（MLC2v，MYH7）and atrial-specific marker（NR2F2，KCNA5）. Cell viability and calcium transient were assessed to evaluate cytotoxicity and cellular electrophysiological alterations caused by different drugs（terfenadine，sotalol and ibutilide）. Result：The results of flow cytometry，immunofluorescent staining and qRT-PCR demonstrated that ventricle-specific markers were highly expressed in RAi group while atrial-specific markers were expressed in RA group. By assessing calcium transient and cell viability，we proposed a drug assessment platform using hiPSC-ACM and hiPSC-VCM. After treated with terfenadine，cytotoxic effects occured at a concentration of 100 μmol/L both in hiPSC-ACM and hiPSC-VCM. Meanwhile，both sotalol and ibutilide exhibited cardiotoxicity potential at a concentration of 1 mmol/L. In the following calcium transient assessment，the amplitudes of calcium transient were significantly decreased in hiPSC-ACM after 1 μmol/L sotalol and 1 μmol/L ibutilide treatment，while in hiPSC-VCM，the amplitude showed no significant difference in such concentrations. The amplitudes of calcium transient were significantly decreased in both groups when the concentration of terfenadine reached to 1 mmol/L. Conclusion：By modulating retinoic acid signaling during hiPSC differentiation，we generated atrial and ventricular cardiomyocytes. Human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes were useful models for assessing cardiotoxicity of drugs by detecting calcium transient.

Abstract:Department of Hematology，the Affiliated Hospital of Jiangsu University，Zhenjiang 212001，China

Abstract:Objective：To investigate the Alzheimer disease（AD）-like changes caused by methamphetamine（METH）exposure，and to elucidate the role of L-type calcium channels in this pathological change. Methods：After primary cultured neurons were exposed to METH（0，30，100，300，and 1 000 μmol/L），Western blotting assay was performed to investigate the expression of AD-like pathological protein amyloid precursor protein（APP）and p-Tau with or without the treatment of nifedipine. Results：After METH treatment，APP and p-Tau increased in a dose-dependent manner. Meanwhile，with a certain concentration of METH cultured with the neurons，the level of APP and p-Tau was increased in a time-dependent manner. After pre-incubation with the calcium channel inhibitor nifedipine（NIF），the METH-induced AD-like changes were significantly improved. Conclusion：METH exposure can cause AD pathological protein changes and L-type calcium channel ihhibitor can partially reverse the adverse changes，therefore，L-type calcium channel may be a potential intervention target for METH with the potential intervention value.

Abstract:Objective：To investigate the effect of urantide，a urotensin Ⅱ receptor antagonist，on hepatic p120-catenin（p120ctn）expression in acute liver failure（ALF） mice. Methods：A total of 24 male Balb/c mice were randomly divided into the healthy control group，pretreatment control group，ALF model group and pretreatment ALF group（6/group）. ALF model mice were induced by lipopolysaccharide（LPS） 50 μg/kg combined with D-galactosamine（D-GalN） 800 mg/kg intraperitoneal injection of ALF，and control mice were injected with an equal volume of 0.9% sodium chloride solution；the pretreated ALF group was given a 0.6 mg/kg urantide tail vein injection 30 min before modeling；the pretreatment control group was replaced with an equal volume of 0.9% sodium chloride solution. And then，liver specimens were collected after 12 h. The levels of hepatic p120ctn mRNA and protein were detected by real-time PCR and Western blot analysis. Results：There was no significant difference in the relative expression levels of p120ctn mRNA and protein between the healthy control group and the pretreatment control group，while the relative expression level of p120ctn mRNA and protein in the healthy control group and the pretreatment ALF group was significantly higher than that in the ALF model group（P < 0.05）. Conclusion：The inhibition of p120ctn expression may be associated with the activation of UⅡ/UT signaling system in LPS/D-GalN-induced ALF mice.

Abstract:Objective：This study aimed to investigate the role of receptor for advanced glycation end products（RAGE）in improving myocardial ischemia and reperfusion after remote ischemic postconditioning. Methods：Thirty-four C57/B6 mice aged 8-9 weeks were randomly divided into six groups：the sham operation group（sham），the FPS-ZM1 control group（FZM1），the ischemia and resperfusion group（IR），the FPS-ZM1 intervention group（FZM1+IR），the RIPostC group（RIPostC+IR）and the RIPostC intervention group（RIPostC+FZM1+IR）. The anterior descending coronary artery was ligated to create myocardial ischemia-reperfusion model of mouse. The left ventricular ejection fraction（LVEF）and left ventricular shortening fraction（LVFS）of mice were detected by mouse cardiac ultrasonography，and the inflammatory factor IL-6，NF-κB P65 protein and RAGE protein were detected by enzyme-linked immunoassay（ELISA）and Western blotting. Results：①RIPostC significantly increased LVEF（P　<　0.01）and LVFS（P　< 0.01）compared with the I/R group. ②Compared with the IR group，RIPostC significantly reduced the expression of RAGE（P　<　0.001）. ③RIPostC significantly reduced the expression of NF-κB P65（P　<　0.001）and IL-6（P　<　0.001）compared with the I/R group. Conclusion：RIPostC was effective in protecting against myocardial ischemia-reperfusion injury. The cardioprotective effects of RIPostC may be achieved directly by inhibiting the expression of RAGE and thereby reducing the inflammatory response.

Abstract:Objective：To investigate the effects of up-regulation of hepatoma-derived growth factor（HDGF） expression on the biological behavior of glioma cells in vitro. Methods: The effects of overexpression of HDGF on the proliferation of malignant glioma cell line DBTRG were examined by MTS and bromodeoxyuridine（BrdU） incorporation experiments. The effects of HDGF up-regulation on the migration and invasion of DBTRG in vitro were investigated by Wound-healing assay，Transwell assay and Matrigel invasion assay，respectively. Western blot and immunofluorescence were used to determine the expression and subcellular localization of cell adhesion molecules. Results: There was no significant difference in the proliferation rate and the proportion of BrdU positive cells between the overexpressing group and the control group. Compared with the control group，the numbers of migration and invasion cells in the HDGF overexpression group were significantly increased. In addition，overexpression of HDGF increased N-cadherin expression but decreased β-catenin protein levels. Furthermore，HDGF did not affect the nuclear translocation of β-catenin，but promoted its phosphorylation. Conclusion: HDGF enhances migration and invasion of DBTRG glioma cells in vitro by promoting β-catenin phosphorylation.

Abstract:Objective：To investigate the differential expression profile and bioinformatics analysis of microRNA（miRNA） in hepatic stellate cells（HSCs） activated by platelet-derived growth factor BB（PDGF-BB） inhibited by psalmotoxin-1（PcTx-1），blocker of acid-sensing ion channel 1a（ASIC1a）. Methods：HSCs in logarithmic growth period were divided into the control group，the model group and the PcTx group. The control group was not treated，after the PcTx group was stimulated by PcTx-1 for 1 h，the model group and the PcTx group were cultured with PDGF-BB（10 ng/mL）medium for 24 h. RT-PCR and Western blot were used to detect the expression of α-smooth muscle actin（α-SMA），collagen-Ⅰ and ASIC1a，and MTT was used to detect the proliferation of cells. These results verified whether HSCs were activated and whether PcTx-1 reduced the expression of ASIC1a. High throughput sequencing of total RNA was carried out after quality control，and differential miRNAs were screened. The target genes of differential miRNAs were predicted by miRanda algorithm，then the functional significance of miRNA target genes and metabolic pathway involved in miRNA were analyzed. Results：After blocking ASIC1a by PcTx-1，the expression of ASIC1a decreased，and the expressions of α-SMA and collagen-Ⅰ in HSCs activation were obviously reduced. It suggested that PcTx-1 can block the activation of HSCs induced by PDGF-BB. In the miRNA expression profile of PDGF-stimulated HSCs，there were 38 differentially expressed miRNAs，of which 6 were up-regulated and 32 were down-regulated（P < 0.05）. After ASIC1a was blocked by PcTx-1，there were 17 differentially expressed miRNAs in the miRNA expression profiles between the PcTx group and the model group，including 1 up-regulation and 16 down-regulation（P < 0.05）. Conclusion：The differential expression profile of miRNA in HSCs activated by PDGF-BB inhibited by PcTx-1 in high-throughput screening，providing new targets and ideas for the study of pathogenesis of liver fibrosis.

Abstract:Objective： To explore the distribution of T lymphocyte subsets in peripheral blood of patients with mantle cell lymphoma（MCL），and evaluate correlation with clinical baseline characteristics and its prognostic value. Methods：The clinical data of 92 newly diagnosed MCL patients from 2006 to 2017 were analyzed retrospectively. The prognostic stratification was performed using a simplified MCL international prognostic index sMIPI. The T lymphocyte subsets，including the absolute number of CD4+ T lymphocytes（ACD4C）and the absolute number of CD8+ T lymphocytes（ACD8C）were analyzed by flow cytometry. Comparisons of T lymphocyte subsets as continuous parameters in different groups were described using Mann-Whitney U test and Kruskal-Wallis. Kaplan-Meier method was used to survival analysis，and the Cox proportional hazards models were used for the estimation of prognostic factors. Results：The median follow-up was 51 months（12-150 months），and the median overall survival（OS）in 92 patients was 44 months. The OS rate at 1，3 and 5 years was 72%，45% and 37%，respectively. In our cohort，patients with high ACD4C（>0.5×109/L）had longer PFS and OS（P=0.009，P=0.004），while patients with low CD4+/CD8+ ratio（≤1.2）had unfavorable PFS and OS（P=0.025，P=0.009）. Univariate Cox regression indicated that ECOG ≥2（P=0.021），B symptoms（fever，night sweats or weight loss）（P=0.001），elevated LDH（P=0.027），high sMIPI score（P=0.004），low ACD4C（P=0.013）and low CD4+/CD8+ ratio（P=0.030）correlated with shorter PFS，while the inferior OS was associated with B symptoms（P<0.001），high sMIPI score（P=0.004），elevated LDH（P=0.040），low ACD4C（P=0.006）and low CD4-/CD8+ ratio（P=0.012）. Multivariate Cox regression showed that B symptoms（P=0.006）and low ACD4C（P=0.001）were the independent prognostic factors of PFS；B symptoms（P=0.003），high sMIPI score（P=0.047），low ACD4C（P=0.001），low CD4+/CD8+ ratio（P=0.031）were the independent prognostic factors of OS. Conclusion：Low ACD4C and low CD4+/CD8+ ratio were associated with unfavorable prognosis in MCL patients. ACD4C level and CD4+/CD8+ ratio proved to be convenient and effective predictors of prognosis in patients with MCL.

Abstract:Objective：To study the expression of phosphoglycerate mutase1（PGAM1）in salivary adenoid cystic carcinoma（SACC）and its clinical and biological significance. Methods：PGAM1 expression was detected by immunohistochemistry in 31 cases of SACC and 25 normal glands，and the correlation with the clinical pathology parameter was analyzed. The highly metastatic human salivary adenoid cystic carcinoma cell line（SACC-LM）was transient transfected by PGAM1 siRNA interference clips. Cell cycle changes，cell proliferation，migration，invasion were evaluated. The changes of matrix metalloproteinase（MMP）-2，MMP-9，N-cadherin，E-cadherin and vimentin expression were examined before and after the interference by Western blot. Results：PGAM1 was expressed at a low level in 25 normal glands. In 31 cases of SACC，20 cases（64.5%）expressed PGAM1 at a high level，while 11 cases（35.5%）expressed at a low level. The PGAM1 expression was significantly higher in the solid pattern than that in the tubular and cribriform pattern（P＜0.05）. The expression of PGAM1 had no correlation with gender，age or tumor location（P＞0.05）. The down-regulation of PGAM1 had no effect on the proliferation capability and cell cycle of SACC-LM cells（P＞0.05），while cells migration and cell invasion were inhibited significantly after PGAM1 was down-regulated（P＜0.05）. The expression of MMP-2 and MMP-9 was also down-regulated after PGAM1 was inhibited（P＜0.05）. Conclusion：The expression of PGAM1 was associated with pathological types of SACC；down-regulation of PGAM1 can significantly inhibit the migration and invasion of adenoid cystic carcinoma.

Abstract:Objective：The aim of this study was to determine the change of long non-coding RNA（lncRNA）taurine up-regulated 1（TUG1）and metastasis associated lung adenocarcinoma transcript 1（MALAT1）in patients’ peripheral blood mononuclear cells（PBMCs）with T2DM，expecting to find a biomarker in diagnosing T2DM. Methods：Real-time quantitative PCR（qRT-PCR）was used to detect the expression levels of lncRNA TUG1 and MALAT1 in 60 patients with type 2 diabetes（the diabetes group）and 45 healthy controls（the healthy control group）. The effects of fasting blood glucose，glycated hemoglobin and the course of disease on lncRNA expression were analyzed. Results：The results of qRT-PCR showed that the relative expression level of lncRNA TUG1 in PBMC of T2DM patients was 6.25 times of the healthy control group（P < 0.05），and the relative expression level of lncRNA MALAT1 in PBMC of T2MD patients was 3.98 times of the healthy control group（P < 0.05）；In PBMC of patients with type 2 diabetes，the expression level of lncRNA MALAT1 and TUG1 rised with the increasing of fasting blood glucose and glycated hemoglobin（P < 0.05）；Meanwhile，the expression level of lncRNA TUG1 and MALAT1 also increased with the prolongation of the patient’s disease course（P < 0.05）；The ROC curve showed that the area under the curve of lncRNA TUG1 was 0.898（95% confidence interval 0.826-0.970，P < 0.001），and the area under the curve for lncRNA MALAT1 was 0.715（95% confidence interval 0.583-0.846，P < 0.001）. Conclusion：The expression of lncRNA TUG1 and MALAT1 in PBMC of patients with T2MD was elevated，and it was related to blood glucose level and course of disease. The expression level of lncRNA TUG1 and MALAT1 may be used as a biomarker for diagnosis and evaluation of the condition of patients with T2MD.

Abstract:Objective：To analysis the relationship of poorly differentiated clusters（PDC）and tumor buddings（TB）in colorectal adenocarcinoma and MUC1 expression in PDC and TB. To explore the correlation and biologic characteristic of PDC and TB in colorectal carcinoma. Methods：The identification and grading of PDC in 183 cases of colorectal adenocarcinoma were observed by HE staining. The identification and grading of TB were examined by CK immunohistochemical staining. MUC1 expression of PDC and TB was detected by EnVision immunohistochemical method. Results：Among 183 cases of colorectal adenocarcinoma，the detection rate of PDC was 56.8%（104/183），intratumoural budding（ITB） was 54.1%（99/183），and peritumoural budding（PTB） was 62.3%（114/183）. There was a positive correlation between PDC and ITB，PDC and PTB in colorectal adenocarcinoma（P < 0.001）. PDC grade was positively correlated with PTB grade（P < 0.05），but not with ITB grade（P > 0.05）. MUC1 was mainly expressed in the lumen margin and/or cytoplasm in the main body of the tumors，while in PDC and TB，MUC1 was expressed in the reverse mode，i.e. I/O mode. The reverse expression rates of MUC1 in PDC，ITB and PTB were 55.8%，58.6% and 54.4%，respectively. Conclusion：PDC and TB，especially PTB，were closely related in colorectal adenocarcinoma which suggested that their biological behaviors were closely related. Reverse expression patterns of MUC1 in PDC and TB suggested that they may have the characteristics of micropapillary carcinoma，which may be different stages of micropapillary carcinogenesis.

Abstract:Objective：To study the relationship between the changes of pulse oxygen saturation（SpO2），pulse rate，and pulmonary function in daily activity of patients with chronic obstructive pulmonary disease（COPD），and to evaluate the value of pulse oxygen saturation monitoring during daily activity in distinguishing stable COPD and acute exacerbation of chronic obstructive pulmonary disease（AECOPD）. Methods：A total of 64 patients with stable COPD and 54 patients with AECOPD were enrolled，and 24 subjects without respiratory diseases were selected as control group. Basic data were collected and the changes of SaO2 and pulse rate were monitored during daily activity. The difference of SpO2 and pulse rate in each group was compared，and the correlation between SpO2，pulse rate and pulmonary function in the stable COPD group was analyzed. Results：①During daily activity，the difference of SaO2 index（including the rest SpO2，the average SpO2，the lowest SpO2（SpO2L），the variation of SpO2（ΔSpO2），ΔSpO2 percentage，SpO2L/ΔSpO2）between AECOPD group and stable COPD group or control group was significant（P < 0.05）. ②ΔSpO2 ≥6.50%，ΔSpO2 percentage ≥ 6.20% had a certain diagnostic value for AECOPD. ③The difference of SpO2L/ΔSpO2 between severe COPD and mild to moderate was statistically significant. ④The average pulse rate of stable COPD was negatively correlated with FVC%pred. The average SpO2 was positively correlated with FEV1，but the other indexes were not significantly correlated with pulmonary function. Conclusion：①Patients with AECOPD were more likely to experience hypoxemia during daily activity，and the SpO2 drop is obvious. ②After excluding cardiovascular diseases and other lung diseases in patients with COPD，ΔSpO2 and ΔSpO2 percentage could be used as monitoring indicators for early AECOPD management. ③The decline in pulse oxygen saturation during daily activity in patients with stable COPD may be related to lung function impairment.

Abstract:Objective：This study aimed to study the factors influencing the human immunodeficiency virus（HIV）couple testing among men who have sex with men（MSM），to provide the basis for formulating HIV couple testing strategies for MSM population. Method：①A 1∶2 matching analysis was conducted among MSM who did couple testing identified at the survey on AIDS prevention and intervention in Nanjing from May to October in 2017，and the control group was among the people who did not do the couple testing in the same survey of the same period of time. Each case was matched by age（±5），sexual orientation and marital status. ②The questionnaire design was based on the information of demography and behavioristics，using network survey. ③The data was screened by Excel first，then the logistic regression model is used for univariate and multivariate analysis. Results：A total of 73 patients were tested for sexual partners，while 146 subjects were matched in the control group. Univariate analysis showed that the number of previous HIV tests（χ2=6.195，P＜0.05），the number of previous HIV self-testing（χ2=33.658，P＜0.05）and the sex role of “0”（χ2=5.223，P＜0.05）had statistical significance with the sexual partner test. Multivariate analysis showed that the number of HIV self-testing in the past（OR=8.502，95%CI：3.474，20.805）and the sex role of “0”（OR=0.397，95%CI：0.163，0.969）were statistically significant. Conclusion：The number of HIV self-testing in the past was the promoting factor of couple testing，and the sex role of “0” was the obstacle factor for the testing.

Abstract:Objective：To compare the effect of ice water cooled cycle microwave ablation and normal temperature water cooled cycle ablation on the morphology of ablation area. Methods Nine pieces of fresh isolated porcine livers，weighing 4.5-5.2 kg. The experiment was divided into ice water cooled cycle group and nomral temperature water cooled cycle group. Each liver was ablated with normal temperature water cycle and ice water cooled cycle at the same time，at different ablation power（40，60，80 W），with different duration（5，8，10 min）. After ablation，the liver tissues were cut along the needle path. The longitudinal diameters（LD），transverse diameters（TD） and preshoot diameters（PD） of the ablation areas were measured，and the spherical ratios（SR） were calculated. Results：Descriptive approach was used for preliminary reporting. Compared with the normal temperature group，the LD of the ice water group was relatively shortened by 13%，10%，12%，15%，11%，13%，8%，8%，while the TD and PD were not obviously changed，so the SR was relatively increased by 22%，21%，7%，14%，1%，1%，11%，5%，4%. In addition，at the same power，the improvement of SR was more obvious in short duration time（5 min）. While at the same duration time，the improvement of SR was more obvious at low power（40 W）. Conclusion：This experiment preliminarily hints that microwave ablation with ice water cold cycle may increase the SR by shortening the LD of the ablation area，especially in the case of low power and short time. But further experiments are needed to obtain more accurate results.

Abstract:The SAM pointed domain containing ETS transcription factor（SPDEF） is an important member of the E26 transformation-specific transcription factor family. SPDEF is initially identified in prostatic epithelium and considered to be involved in prostate development and prostate cancer progression. However，recent studies revealed that SPDEF is also expressed in the respiratory system. It is pretty crucial for the differentiation of airway epithelium，especially for the proliferation and metaplasia of goblet cells. Due to the importance of goblet cells in respiratory health，extended understanding of SPDEF function not only helps us to illuminate the mechanism of abnormal mucus secretion but also open new perspectives for the management of these related diseases. This review mainly focuses on the recent data which studied the role of SPDEF in goblet cell control，highlighting a novel profile of SPDEF as a new target in mucus secretion moderation.

Abstract:Objective：This meta-analysis sought to assess the overall diagnostic efficacy of long-noncoding RNA（lncRNA）MALAT-1 in lung cancer. Methods：Relevant studies were searched and obtained through the online PubMed，EMbase，EBSCO，CNKI，and Wanfang databases. Data were extracted and study quality of the included studies was assessed. The pooled effect sizes were synthesized and the summary receiver operator characteristic（SROC）curve was plotted using a bivariate meta-analysis model. Sensitivity analysis and meta-regression test were undertaken to identify the potential causes of study heterogeneity. Publication bias was judged by Deeks’ funnel plot asymmetry test. The Z test was used to analyze the difference among pooled AUC values. Results：Five studies comprised of 433 lung cancer patients and 384 paired controls were included. The SROC displayed that the pooled sensitivity，specificity，and area under curve（AUC）of MALAT-1 testing for diagnosing lung cancer were 0.71（95% CI：0.67-0.74），0.82（95% CI：0.79-0.84），and 0.89，respectively. Stratified analyses based on pathological type showed that MALAT-1 testing yielded an AUC of 0.91 in identifying non-small-cell lung cancer（NSCLC），and the AUC was 0.93 in confirming lung squamous cell carcinoma，which was better than that in lung adenocarcinoma（AUC=0.84；Z=1.97，P < 0.05）；moreover，serum-based MALAT-1 testing achieved an efficacy better than plasma-based analysis（AUC：0.94 vs. 0.88；Z=8.96，P < 0.01）. Study based on ethnicity showed that MALAT-1 harbored an AUC of 0.89 in Asian-based analysis，which was equal to that in Caucasian-based analysis（AUC=0.82；Z=1.11，P > 0.05）. Deeks’ funnel plot asymmetry test manifested no clear publication bias among studies（P=0.865）. Conclusion：Circulating MALAT-1 testing reveals promising diagnostic efficacy in identification of lung cancer and therefore might be popularized as auxiliary biomarkers for NSCLC，especially for lung squamous cell carcinoma detection.