Abstract:Objective：This study aims to investigate the expression and significance of tumor suppressor SUFU（Suppressor of Fused）in ovarian cancer. Methods：We detected the expression of SUFU in human ovarian cancer and adjacent tissues by immunohistochemistry，and verified the results in large samples and at the cellular level. Then MTT，EdU，flow cytometry，Western blot，Transwell，cloning formation experiments were used to detect cell proliferation，apoptosis，epithelial-mesenchymal transition（EMT），migration and invasion after regulating the expression of SUFU in the human normal ovarian epithelial cell line IOSE80 and the ovarian cancer cell line SKOV3 respectively. Meanwhile，the protein and mRNA levels of target gene GLI1 in the Sonic Hedgehog（SHH）signaling pathway were detected by Western blot and quantitative real-time PCR（qPCR）. Results：In three ovarian cancer tissue samples，SUFU in cancer tissues was downregulated compared to adjacent tissues，and this result was also confirmed in large samples of the TCGA database. In addition，at the cellular level，SUFU was higher in the human normal ovarian epithelial cell line IOSE80 than in the ovarian cancer cell line SKOV3. Further overexpression of SUFU in SKOV3 cells can effectively inhibit proliferation，EMT，colony formation，migration and invasion，and promote apoptosis；on the contrary，downregulation of SUFU in IOSE80 cells can promote cell EMT，migration and invasion. At the same time，GLI1 mRNA and protein levels were significantly

Abstract:Objective：This study aims to identify the molecular etiology of two non-syndromic hearing loss families，and to use zebrafish to analyze the function of WFS1 on the occurrence of hearing loss. Methods：By using targeted capture sequencing technology，exon sequencing analysis was performed on two families to identify candidate pathogenic genes. The function of human WFS1 gene and the homology of WFS1 gene between zebrafish and human were analyzed by bioinformatics. The temporal and spatial expression characteristics of zebrafish wfs1a and wfs1b were analyzed by whole-embryo in situ hybridization and quantitative PCR. Results：Exon sequencing and co-segregation analysis of two families suggested that the heterozygous mutation of the WFS1 c.2036~2038delAGG（p.680delE） and c.1957C>T（p.653R> c）was the molecular pathogenesis basis of the pedigree JSNY-021 and JSNY-033，respectively. Quantitative PCR and whole-embryo in situ hybridization results showed that zebrafish wfs1a and wfs1b showed different spatio-temporal expression characteristics at different embryonic development stages. Bioinformatics analysis suggested that wfs1b had a closer evolutionary distance and higher homology with human WFS1. Conclusion：This study confirmed that the molecular etiology of two autosomal dominant non-syndrome hearing loss families are WFS1 mutations，which expanded the gene mutation spectrum of hereditary hearing loss. The expression of wfs1a/wfs1b in the embryonic development of the zebrafish model has obvious spatio-temporal specificity. Wfs1b is the direct homologous gene of human WFS1. The results of this study not only provide support for the molecular diagnosis of hearing loss，but also lay a foundation for further study on the mutagenesis mechanism of WFS1.

Abstract:Objective：This study aims to explore the function of CASK gene on the regulation of diabetes mellitus in successfully constructed and identified mice model by conditionally CASK knockout in islet β-cells. Methods：CASKloxp/- female mice and CASKloxp/Y male mice were crossed to obtain CASKloxp/loxp female mice and CASKloxp/Y male mice，and then the male mice with conditioned islet beta cells expressing Cre recombinase and CASKloxp/loxp female mice were crossed to obtain CASKloxP/YMIP-Cre male mice and CASKloxP/-MIP-Cre female mice. Mice with CASKloxP/YMIP-Cre genotype are the model mice needed for this experiment. Mice were tailed 1-2 weeks after birth，and their genotypes were identified by PCR. The expression of Cre recombinase was induced by intraperitoneal injection of tamoxifen at 4-5 weeks. Real-time fluorescence quantitative PCR and Western blot were used to verify the knockout effect of CASK gene. Results：Twenty-eight male mice with CASKloxP/YMIP-Cre genotype were obtained after 10-month cross- breeding. Genotype validated by PCR analysis were CASKloxP/YMIP-Cre. The expression of CASK in islets decreased significantly in CASKloxP/YMIP-Cre mice detected by real-time fluorescence quantitative PCR and Western blot. Conclusion：By using Cre-loxp recombination system，the mice model with conditionally CASK deleted in islets was successfully constructed，which provided a research platform for studying the role of CASK gene in the pathogenesis of diabetes mellitus.

Abstract:Objective：This study aims to examine the effects of signal transducer and activator of transcription 3（STAT3） on the transcription and expression of programmed death ligand 1（PD-L1）gene in human glioma cells and screen the possible STAT3-binding elements within PD-L1 gene promotor. Methods：U251 cells were cultured and treated with STAT3 inhibitor（BP-1-102） or DMSO. The expression of PD-L1 protein on the surface of U251 cells was examined by flow cytometry after 9 h. The luciferase reporter plasmid of full-length PD-L1 gene promotor（pGL3-PD-L1-FL）was transfected into U251 cells，and then cells were treated with BP-1-102 or DMSO. The luciferase activity in U251 cells was examined after 9 h. The luciferase reporter plasmids of full-length or truncated PD-L1 promoter（pGL3-PD-L1-FL，pGL3-PD-L1-1，pGL3-PD-L1-2，pGL3-PD-L1-3，pGL3-PD-L1-4）and the plasmid of pCMV-STAT3 were co-transfected into HEK293 cells. Then，the luciferase activity was detected to screen the STAT3-binding elements. Furthermore，bioinformatics software was used to predict the STAT3-binding elements in the promoter of PD-L1 gene，and mutation experiments were carried out to determine the validity of these binding elements. Results：BP-1-102 could significantly down-regulate the gene transcription and protein expression of PD-L1 in U251 cells. In addition，the plasmids of pGL3-PD-L1-FL or pGL3-PD-L1-1~4 and pCMV-STAT3 were co-transfected into HEK293 cells，and then the luciferase activity in different groups was determined. The result displayed that the activity of pGL3-PD-L1-4 was much lower than that in others，indicating that the region of PD-L1 promoter（-200~0 nt）might contain STAT3-binding elements. Bioinformatics software predicted that the region might contain two STAT3-binding elements，that are located at -194~-184 nt and -135~-125 nt. Further studies revealed that mutation of -194 ~-184 nt and -135~-125 nt elements especially the combined mutation could significantly down-regulate the luciferase activity of pGL3-PD-L1-FL. Conclusion：STAT3-binding elements within PD-L1 gene promotor were successfully screened out，which could be beneficial to further studies about the STAT3-related transcription regulation of PD-L1 gene in glioma cells.

Abstract:Objective：This study aims to investigate the effect and mechanism of S100 calcium binding protein A16 （S100A16） on migration of gastric cancer cells. Methods：Gastric cancer cell lines SGC-7901 and MGC-803 with stable overexpression of S100A16 were constructed by lentivirus infection . Wound healing assay and Transwell assay were used to analyze the migration of gastric cancer cells. Liposome transfection method was used to transfect the S100A16 siRNA into SGC-7901，and expressons of E-Cadherin and Vimentin were detected by Western blot. The interaction between S100A16 and zonula occludens 2（ZO-2） was detected by co-immunoprecipitation. The location of S100A16 and ZO-2 was detected by immunofluorescence. Results：S100A16 expression was higher in gastric cancer cells than in normal gastric epithelial cells. Cell lines with S100A16 overexpression were stably constructed. Overexpression of S100A16 promoted the migration of gastric cancer cells. E-Cadherin protein increased，S100A16 and Vimentin proteins decreased in SGC-7901 cells by transfection of S100A16 siRNA. S100A16 interacted with ZO-2 in SGC-7901 cells. Conclusion：Overexpression of S100A16 promotes the migration of gastric cancer cells. Its mechanism may be through the interaction of ZO-2.

Abstract:Objective：This study aims to investigate the anticancer activities and molecular mechanisms of resveratrol on human gastric cancer BGC823 cells. Methods：Growth of BGC823 cells affected by resveratrol was detected by MTT assay. Effects of resveratrol on apoptosis，reactive oxygen species levels and mitochondrial membrane potential of BGC823 were determined by flow cytometry. Expression changes of cleaved caspase-3 and cleaved caspase-9 proteins in BGC823 cells affected by resveratrol were detected by Western blot. Results：The results of MTT showed resveratrol inhibited the proliferation of BGC823 in a time-and dose-dependent manner. Treatment with 31.25-500.00 μmol/L resveratrol for 24 h and 48 h induced apoptosis of BGC823 cells，and the apoptosis rate reached （40.42±2.64）% and （75.02±2.36）% in resveratrol concentration of 500 μmol/L at 24 h and 48 h，respectively. Flow cytometric analysis showed that BGC823 cells treated with resveratrol were accumulated in ROS levels in cells for 24 h. Compared with the control group，BGC823 cells showed a significantly decreased in mitochondrial membrane potential after treated with 62.50-500.00 μmol/L resveratrol for 24 h（P < 0.05）. Western blot showed that expressions of cleaved caspase-9 and cleaved caspase-3 protein in BGC823 cells were increased after resveratrol treatment in a dose-dependent manner. Conclusion：Resveratrol can significantly induce cell apoptosis，which is related to the increase of ROS，the decrease of mitochondrial membrane potential and the regulation of cleaved caspase-9 and cleaved caspase-3 expression.

Abstract:Objective：This study aims to investigate the protective mechanism of metformin（Met）on rat cardiomyocytes H9C2 injury induced by lipopolysaccharide（LPS）. Methods：Rat cardiomyocytes H9C2 were divided into Control group，LPS group，Met of different concentrations added with LPS group，according to different treatments. Cells were cultured for 24 hours. MTT was used to detect the cell viability，and intracellular reaction oxygen species and apoptosis were detected by the flow cytometry. The protein levels of Caspase3，BCL2，BAX，t-AMPK（adenosine 5′-monophosphate -activated protein kinase），p-AMPK（phosphorylated adenosine 5′-monophosphate -activated protein kinase），Beclin1 and Parkin were measured by Western blot. Results：Compared with LPS treatment alone，combine of Met and LPS decreased cell viability and apoptosis induced by LPS. The expressions of BCL2 and BAX proteins were decreased，while the Caspase3 protein expression was not decreased. And the expressions of p-AMPK，Beclin1 and Parkin proteins were increased，and the level of reactive oxygen species was deduced. Conclusion：Met may increase autophagy and reduce the production of reactive oxygen species by activating AMPK pathway，alleviate the damage of LPS to cells and protect myocardial cells.

Abstract:Objective：This study aims to investigate the effect and mechanism of fucoidan inhibiting inflammatory bowel disease（IBD）. Methods：Mice were randomly divided into experimental group and control group（n=5，each group）. The experimental group was intragastrically with fucoidan，meanwhile，the control group was given drinking water. Two weeks later，both groups drank dextran sulfate sodium salt（DSS） to make mouse model of IBD. Body weight，spleen weight，colon length and weight of mouse were measured. Pathological changes of colon tissue was observed by HE staining，stool property was observed and scored，and mRNAs of inflammatory factors，chemokines and tight junction proteins were detected by real-time fluorescence quantitative PCR. Results：Intestinal inflammation related indexes were better in experimental group treated with fucoidan than in the control group，and the degree of inflammation was reduced. Conclusion：Fucoidan can prevent and inhibit the occurrence of IBD. This study will provide a new method and evidence for the prevention and treatment of IBD.

Abstract:Objective：This study aims to identify human herpesvirus 6（HHV-6） binding partner for glycoprotein 96（gp96），and analyze the effect of their interaction on HHV-6 infection. Methods：Immunoprecipitation，Western blot and confocal microscopyassays were used to identify and verify the interaction between gp96 and HHV-6 glycoprotein B（gB）. Effect of Gp96 on gB maturation was analyzed using exosome samples. Results：Immunoprecipitation results showed that HHV-6 gB interacted with gp96；gB and gp96 were colocalized，which was detected by confocal microscopy assays. Western blot results confirmed that overexpression of gp96 affected the maturation of gB. Conclusion：The gp96 is a binding partner for HHV-6 gB，and gp96 affects the maturation process of gB.

Abstract:Objective：This study aims to investigate the effects of ovarian tissue endogenous peptide 1 derived from AHNK2 protein（P1DA）on the proliferation，migration，invasion and apoptosis of ovarian cancer cell lines of SKOV3 and OVCAR3. Methods：SKOV3 and OVCAR3 cells in logarithmic growth phase were treated with P1DA. The cell activity was detected by CCK-8 assay to explore the effect of P1DA on the proliferation of SKOV3 and OVCAR3 cells. Transwell experiment was performed to detect the migration and invasion of SKOV3 and OVCAR3 cells treated with P1DA. Flow cytometry analysis was performed to analyze the apoptosis of SKOV3 and OVCAR3 cells treated with P1DA. And the effects of P1DA on apoptosis of ovarian cancer cells were analyzed. Results：The cell activity of SKOV3 and OVCAR3 cells treated with P1DA was significantly increased，the migrated and invaded cells in chamber were increased，and no difference was found in apoptosis of cells after treated with P1DA. Conclusion：Ovarian tissue endogenous peptide P1DA can promote the proliferation，migration and invasion of SKOV3 and OVCAR3，but has no effect on cell apoptosis.

Abstract:Objective：This study aims to investigate the expression of miR-588 in breast cancer and its effects on proliferation，migration and invasion of breast cancer cells. Methods：The miR-588 was synthesized by chemical synthesis（miR-588 mimic），and the over expression rate of miR-588 was verified. MTT and clonogenic assay were used to detect the effect of miR-588 on the proliferation of breast cancer cells of MCF7 and MDA-MB-231. The effects of miR-588 on migration and invasion of MDA-MB-231 cells were detected by scratch healing test and Transwell invasion test. Results：MiR-588 expression in breast cancer tissues were lower than those in adjacent normal tissues and normal breast epithelial cells；Overexpression of miR-588 could inhibit proliferation of MCF7 and MDA-MB-231 cells；upregulation of miR-588 could significantly inhibit the migration and invasion of MDA-MB-231 cells. Conclusion：The expression of miR-588 is low in breast cancer. Overexpression of miR-588 can inhibit the proliferation and invasion of breast cancer cells.

Abstract:Objective：This study aims to investigate the value of procalcitonin（PCT） and C-reactive protein（CRP） in the early treatment of cirrhosis patients with suspected abdominal infection. Methods：One hundred and twenty eight cirrhotic patients whose ascites cell count results meet the diagnostic criteria of spontaneous bacterial peritonitis（SBP） were enrolled in this study. PCT and CRP in ascitic specimens were tested，and patients were divided into SBP group and non-SBP group according to the bacterial culture results of ascitic specimens. The correlation between PCT，CRP expression and the occurrence of SBP were analyzed；the difference of PCT and CRP expression in patients infected with gram-negative bacteria（G-） and gram-positive bacteria（G+）were compared. The ROC curve was used to find the cut-off value of PCT and CRP level in SBP diagnosis. According to cut-off values of PCT and CRP used as the treatment standard，128 patients were re-grouped and the accuracy of antibiotic treatment was analyzed. Results：PCT level was much lower in non-SBP group than in SBP group（P < 0.05），and there was no statistical difference in CRP level between non-SBP group and SBP group（P > 0.05）. PCT level were higher in G- group than in G+ group（P < 0.05），and there was no statistical difference in CRP level between G- group and G+ group（P > 0.05）. The area under the ROC curve of PCT and ROC were 0.670 and 0.684，the cut-off value were 0.290 ng/mL and 34.0 mg/L，respectively. Under the situation without inflammatory biomarkers supplied，with PCT>0.290 ng/mL and with CRP>34.0 mg/L，the accuracy of antibiotic therapy were 67.2%，88.6% and 81.6%，respectively. Conclusion：PCT and CRP detection can provide useful information for screening and diagnosing SBP in cirrhosis patients，and elevate the accuracy of antibiotic therapy.

Abstract:Objective：This study aims to describe the different appearances of bone marrow distribution changes adjacent to the sacroiliac joint in both ankylosing spondylitis（AS） patients and volunteer control without seronegative spondy-loarthropathy（SPA） at different ages，and to highlight the potential MR imaging features that may lead to misdiagnosis. Methods：The study included 31 AS patients aged from 14 to 46 years old and 89 volunteers without SPA aged from 17 to 87 years old. AS patients were divided into 3 groups：active stage AS patients were divided group，chronic stage group and endstage group. Volunteers were divided into three groups according to their age：40 years old（adolescent group），≥40~60 years old（middle-aged group）and ≥60 years old（old group）. Distribution of yellow bone marrow in sacroiliac joint was measured after routine MR scanning，and the percentage of yellow bone marrow in sacroiliac joint（fat faction，FF） was calculated. Results：FF value in sacroiliac joint of AS patients in chronic and end-stage was significantly higher than that in active stage，and the differences were was statistically significant（P < 0.001）. There was no significant difference in FF between chronic and end-stage（P=0.636）. In addition，there was no significant difference in the distribution of yellow bone marrow in sacroiliac joint between patients with AS in chronic stage and volunteers with degenerated sacroiliac joint，but the former usually showed localized deposition，while the latter showed diffuse distribution. With the increase of age，the content of yellow bone marrow in sacroiliac joint increased gradually; there was no significant difference in FF value between middle-aged group and old group（P=0.221）; there were significant differences in FF value between adolescent group and middle-aged group or old group（P < 0.001）。Conclusion：By studying the bone marrow distribution changes adjacent to the sacroiliac joints on MRI，it’s helpful to diagnose AS and reduce the misdiagnosis or miss diagnosis.

Abstract:Objective：This study aims to analyze the reasons for the removal of levonorgestrel-releasing intrauterine system（LNG-IUS）and the satisfaction of patients，and to explore how to improve the continuous use rate and strengthen the management of patients with LNG-IUS. Methods：The data of 407 patients who voluntarily removed LNG-IUS from January 2015 to June 2018 in our hospital were retrospectively analyzed. According to the reasons for initial LNG-IUS placement in different clinical application，the patients were divided into the contraceptive group and the treatment group. Reasons for removing the LNG-IUS and the satisfaction of patients were compared between both groups. Results：No pregnancy occurred in both groups. The primary reasons for the removal of LNG-IUS in both groups were symptomatic，including amenorrhea and vaginal drip bleeding，and the continued use rate and satisfaction rate of the treatment group were significantly higher than those of the contraceptive group（P < 0.05）. Compared with the treatment group，more women in the contraceptive group removed LNG-IUS due to hormone-related side effects（P < 0.05），which mainly were facial acne and pigmentation（P < 0.05）. Among women with amenorrhea，the placement time was significantly longer and satisfaction rate was significantly higher in the treatment group than in the contraceptive group（P < 0.05）. Conclusion：Different guiding strategies should be given to patients with different clinical applications of LNG-IUS，including detailed notification of various side effects before putting LNG-IUS，follow-up and timely treatment of various side reactions and complications，so as to reduce the rate of LNG-IUS extraction and improve the satisfaction rate of patients.

Abstract:Objective：To establish an effective and practical method to solve the problem of polymerase chain reaction（PCR）contamination and used it for prenatal diagnosis of folic acid utilization capability. Methods：By adding recognition sequence of a restriction enzyme FOK1 at the 5′ end of one PCR primer，carryover PCR contamination was controlled by adding FOK1 enzyme in PCR reaction mixture which contained the contamination from the last PCR products. Effects of the amount of FOK1 enzymatic on amplification efficiency，components in PCR reaction mixture and the most amounts of contamination which can be controlled were investigated. A mutation of C677T in MTHFR gene was analyzed. Results：A best reaction system provided by FOK1 enzyme specification inhibited PCR amplification. In TaKaRa reaction system，0.5 U of FOK1 enzyme could completely control the contamination of less than 0.1 μL of last amplification products and did not affect the amplification. Otherwise，false positive results were obtained by adding the contamination without FOK1 enzyme. The developed method was successfully applied to the analysis of MTHFR C677T by sequencing. Conclusion：This method is effective and convenient. The reactions of enzyme digestion and amplification were completed in an unclosed tube. Subsequent sequencing reaction is not affected by the method. The method can not only be used in prenatal diagnosis based on PCR amplification，but also widely applied to control PCR amplification in all the fields involved in nucleic acid amplification.

Abstract:Objective：This study aims to investigate the relationship between serum 25-hydroxyvitamin［D25（OH）D］ concentration and hand-foot-and-mouth disease（HFMD），and to evaluate the clinical significance of 25（OH）D concentrations in children with HFMD. Methods：Total 126 children with HFMD were divided into mild HFMD group（84 cases）and severe HFMD group（42 cases）. Another 58 healthy children in the same period were selected as control group. Serum 25（OH）D concentrations were measured in all subjects，and all subjects were divided into 25（OH）D normal（≥75 nmol/L）group，25（OH）D insufficiency（≥50-75 nmol/L）group and 25（OH）D deficiency（< 50 nmol/L）group according to serum 25（OH）D concentration. Serum calcium，D-dimer，lactate dehydrogenase and creatine kinase-MB levels were also measured in children with HFMD. Enterovirus nucleic acid was detected and typed by RT-PCR. Results：Serum 25（OH）D concentrations were generally low in all groups. The average concentration of serum 25（OH）D in severe HFMD group［（50.2±10.2）nmol/L］ was significantly lower than that in healthy control group［（73.1±12.3）nmol/L］and mild HFMD group［（71.2±13.5）nmol/L］；EV71 infection rate in severe HFMD group was significantly higher than that in mild HFMD group. Logistic regression analysis showed that serum 25（OH）D concentration was an independent factor affecting the prognosis of HFMD children. Conclusion：Serum 25（OH）D concentration has clinical value in judging and predicting the progress of HFMD. Further evidence is needed to demonstrate the clinical value of 25（OH）D in diagnosis of HMFD.

Abstract:Objective：This study aims to analyze the incidence of extrauterine growth retardation（EUGR） and related risk factors of late-term premature infants discharged from hospital，so as to provide reference for prevention and treatment of EUGR in the future. Methods：The clinical data of 1 374 mid-late preterm infants enrolled in the NICU from January 2016 to June 1818 in Hainan Maternal and Children’s Medical Center were collected. Infants were divided into EUGR group and non-EUGR group according to the body weight at discharge，and indexes of EUGR group and non-EUGR group were compared. All data were statistically analyzed using SPSS22.0 software. Results：The incidence of EUGR was 26.74% in mid-term preterm infants and 26.14% in late preterm infants. There was no significant difference in the incidence of EUGR between mid-term preterm infants and late preterm infants （χ2 =0.033，P=0.855）.The lower the birth weight was，the higher the incidence of EUGR was. The differences in the incidence of EUGR in preterm infants with different birth weights were statistically significant（χ2 =81.746，P < 0.001）；incidences of brain injury，sepsis and retinopathy of prematurity in the EUGR group were higher than those in the non-EUGR group（P < 0.05）；total enteral nutrition time，oxygen inhalation time，fasting time and hospitalization time were longer in EUGR group than in non-EUGR group（P < 0.001）. Incidences of gestational hypertension，multiple births，IUGR（intrauterine growth retardation） and umbilical cord abnormalities were higher in EUGR group than in non-EUGR group（P < 0.05）. The fetal weight of EUGR group was significantly lower than that of non-EUGR group（P < 0.05）；Logistic regression analysis showed that multiple births，IUGR，umbilical cord abnormalities，birth weight and hospital-stay time were independent risk factors for EUGR. Conclusion：The occurrence of EUGR in mid-late preterm infants is related to multiple births，IUGR，abnormal umbilical cord，low birth weight and long hospital stay. Strengthening perinatal health care，preventing the birth of low-weight infants，reasonable nutritional support，actively preventing high-risk factors and reducing various complications after birth are important measures to reduce the occurrence of EUGR in mid-late preterm infants.

Abstract:Objective：This study aimed to investigate the role of interleukin 17A（IL-17A） in the regulation of bone destruction in experimental periodontitis model. Methods：Male IL-17A knockout C57BL/6 mice and C57BL/6 wild type mice induced with ligation and Porphyromonasgingivalis infection were used to establish experimental periodontitis model in this study. Wild type mice and IL-17A knockout mice were divided into the normal group and the periodontitis group，respectively. The experimental periodontitis model mice were sacrificed 4 weeks after ligation to collect maxillary bones. Micro-computed tomography（Micro-CT），HE staining，TRAP staining，immunohistochemistry and histopathological analysis were performed to reveal the role of IL-17A on bone destruction and inflammation. Results：In the experimental periodontitis mice model induced with Porphyromonasgingivalis infection and ligation，IL-17A knockout mice showed less alveolar bone resorption in the three-dimensional reconstruction of Micro-CT than wild-type mice did. The mesial and distal linear distance from the alveolar bone crest（ABC） to the cementoenamel junction（CEJ） of maxillary second molars in the IL-17A knockout mice was（0.51 ± 0.11）mm and （0.45 ± 0.04）mm respectively，significantly less than those in wild-type mice（P < 0.05），which was（0.61 ± 0.09）mm and（0.65 ± 0.08）mm respectively. In addition，compared to wild-type mice，the mean bone volume fraction in IL-17A knockout mice was 41.88 ± 1.82，significantly higher than that in wild-type mice（36.55 ± 2.08，P < 0.05），and the bone mineral density in IL-17A knockout mice was 84.39 ± 2.11，significantly higher than that in wild-type mice（76.90 ± 2.55，P < 0.05）. On the other hand，IL-17A knockout mice exhibited significantly less alveolar bone resorption in periodontal tissue in HE staining than wild-type mice；TRAP-positive cells and the expression of receptor activator of nuclear factor κB ligand（RANKL） in periodontal tissue were reduced as well. Conclusion：IL-17A may promote the development of periodontitis and alveolar bone resorption.

Abstract:Objective：This study aims to investigate the effect of inflammatory factors on renal injury in periodontitis model of mice. Methods：High or low fat diet-induced periodontitis models of mice were established. Mice were divided into high fat diet without periodontitis group（High+NCPre），high fat diet with periodontitis group（High+Pre），low fat diet with periodontitis group（Low+Pre），low fat diet without periodontitis group（low+NCPre）. The changes of serum total protein，albumin and creatinine in mice were observed. The protein levels of TGF-β1/Samd6 signaling pathway related proteins，MMP-2，MMP-9 and TIMP-1 were detected by Western blot. Changes of tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）levels were detected by enzyme linked immunosorbent assay（ELISA）. Results：Mice fed with high fat diet were significant obesity at the 20th week. Compared with those in Low+NCPre group，the levels of total protein and albumin in serum were significantly decreased in High+NCPre group and High+Pre group（P < 0.05）；MMP-2 and MMP-9 protein expression in kidney were significantly decreased （P < 0.05）；serum creatinine，TNF-α and IL-6 were increased （P < 0.05）；The TIMP-1 protein expression in kidney was significantly increased in High+NCPre group and High+Pre group（P < 0.05）. The expression of TGF-β1 protein（P < 0.05）was increased，levels of Samd6 and E-cadherin protein were inhibited in kidney of High+NCPre group and High+Pre group（P < 0.05）. Compared with those in High+NCPre group，total protein and albumin in High+Pre group were significantly decreased（P < 0.05）；inflammatory factor levels of TNF-α and IL-6，MMP-2 and MMP-9 protein expression decreased；serum creatinine，TGF-β1 and TIMP-1 proteins in kidney increased（P < 0.05）；expression of Samd6 and E-cadherin proteins in kidney decreased（P < 0.05）. Conclusion：Periodontitis aggravates the renal injury of obese mice，which may promote renal fibrosis，inhibit the degradation of extracellular matrix，and promote the process of epithelial-mesenchymal transition by regulating TGF-β1/Samd6 signaling pathway .

Abstract:Objective：Chronic periodontitis patients often suffer from dentin hypersensitivity（DH） due to gingival recession or root surface exposure . The aim of the study is to evaluate the changes of dentin tablets and hypersensitivity teeth after Nd：YAG laser，Gluma desensitizer and their combination treatment. Methods：Forty-eight severe periodontitis teeth were extracted and randomly divided into four groups：Control group，Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group. The Scanning electron microscopy（SEM）was used to observe the opening of dentinal tubules. One hundred DH teeth from 30 patients with periodontitis were selected and randomly divided into four groups as above；Visual analogue scale（VAS）was used to assess the sensitivity of clinical patients after temperature and mechanical stimuli at baseline and one month after treatment. Results：SEM showed that the diameter of dentinal tubules of Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group were smaller than that of the control group（P < 0.05），and the exposure rate was significantly decreased in Gluma +Nd：YAG group（P < 0.05）. The VAS of Nd：YAG laser group，Gluma desensitizer group and Gluma +Nd：YAG group was significantly lower after treatment than before treatment（P < 0.05）. The Gluma +Nd：YAG group obtained the most effective results than any other groups，which differed significantly from the control group（P < 0.05）. Conclusion：Combining Gluma and Nd：YAG to treat DH can effectively reduce dentinal tubules exposed and the sensitivity of DH teeth.

Abstract:Objective：This study aims to explore the clinical efficacy and safety of Shuanghua Baihe Tablet in treatment of erosive oral lichen planus（EOLP），and to provide more ideas and options for treatment of EOLP. Methods：Sixty EOLP patients were divided into two groups randomly. The experimental group was given Shuanghua Baihe Tablets，and the control group was given Transfer Factor Capsules. Both groups were treated with triamcinolone acetonide oral ointment and Xipayi mouth rinse at the same time. The changes of clinical symptoms and signs were compared before treatment and after treatment for one week between both groups. And indexes of blood，liver and renal function before and after treatment were compared. Results：EOLP patients in both of the experimental group and the control group had less erosion area and less pain after treatment of one week（P < 0.05）. The total effective rate was 93.3% in the experimental group and 70.0% in the control group，and there were significant differences in the total effective rate between both groups（P＜0.05）. There was no significant difference in the related indexes of blood，liver and renal function before and after treatment in EOLP patients treated with Shuanghua Baihe Tablets（P > 0.05）. Conclusion：Shuanghua Baihe Tablet is more effective than Transfer Factor Capsule in treatment of EOLP，and it is safe in clinical use.

Abstract:Traditionally，testosterone therapy can promote the growth of prostate cancer cells，so testosterone therapy is not suitable for prostate cancer patients，but the results of recent researches are contrary to the axiom. The current research indicates that the high endogenous androgen levels do not increase the incidence of prostate cancer and do not contribute to disease progression in prostate cancer patients. Androgen saturation model seems to explain this phenomenon；and for the prostate cancer patients with testosterone deficiency（TD），testosterone therapy can improve the quality of life. The role of testosterone on the growth and treatment of prostate cancer is complex，and further exploration is needed. Therefore，this article reviews the recent research on the relationship between testosterone and prostate cancer.