Abstract:Clinical medical research is the core driving force of medical development. In the context of big data，the China government provided institutional guarantee and resource support for the clinical medical research through top-level design. The rapid development of multi-omics and information technology has provided valuable resource accumulation，helped the continuous innovation，and catalyzed the continuous change for China’s clinical medical research. China’s clinical medical research is facing new opportunities. However，China’s biomedical big data still has challenges such as fragmentation of storage and lack of data exchange mechanisms. It is urgent to establish a government-led data sharing mechanism and seek a cooperative research model. In the new era，real-world research relying on big data will gradually become an important source of evidence for clinical intervention. We must strengthen the accumulation of clinical data as well as biological sample，innovate research methods，break the traditional thinking mode，and meet new opportunities and challenge.

Abstract:Corona virus disease 2019（COVID-19）is a new disease characterized by lung damage，involving multiple tissues and organs in the whole body. After the completion of clinical treatment，some patients may have long-term dysfunction，including pulmonary fibrosis，heart，liver，kidney，nerve and immune system. Rehabilitation has a certain beneficial effect in the acute stage，and especially in the recovery stage，including improving respiratory function，exercise endurance，self-care in daily living activities，as well as psychological support，etc. Rehabilitation is not offside or absent. A reasonable rehabilitation program needs scientific research to avoid arbitrary conclusions.

Abstract:Since December 2019，the novel coronavirus pneumonia（coronavirus disease 2019，COVID-19）has spread to all provinces of China and some other regions abroad. As of February 21，2020，the total number of confirmed cases in China has exceeded 70 000. In terms of antiviral drugs，there are still no confirmed effective drugs against COVID-19. The present study aims to introduce the potential effective drugs and related research progress，providing helpful information for improving the cure rate，reducing the mortality and controlling the development of the epidemic.

Abstract:The outbreak of corona virus disease 2019（COVID-19）in Wuhan since December 2019 has caused trouble in the diagnosis and treatment of gastric cancer patients，and also increased the pressure on hospitals. Gastric cancer，as a highly heterogeneous malignant tumor，should be treated with special diagnosis and therapeutic strategy according to its characteristics during the outbreak. And we should adjust guidance and management according to local conditions to reduce the adverse impact of the diagnosis and treatment in gastric cancer patients during the COVID-19 epidemic periods.

Abstract:Coronavirus disease 19 is a respiratory illness that can spread from person to person. The disease was first found in Wuhan，Hubei Province. Due to its high infectivity，this epidemic seriously threaten the global public health. WHO designated this contagion as an international public health emergency on January 30，2020，and officially renamed the disease as coronavirus disease 2019（COVID-19），on February 11，2020. The International Committee on Taxonomy of Viruses（ICTV）named the virus as severe acute respiratory syndrome coronavirus 2（SARS-CoV-2）. This disease has been included in class B infectious diseases and managed as class A infectious diseases by the government of People’s Republic of China. In order to provide ophthalmic medical workers with the understanding of the disease，this article would focus on the characteristics，epidemiology，transmission of SARS-CoV-2. The relationship between other common respiratory viruses and eye infection，and the protection method for ophthalmic medical workers during the outbreak response were also discussed.

Abstract:Objective：This study aims to prepare anti-tyrosine-kinase-like orphan receptor 1 （ROR1） IgG1（ROR1-IgG1）and the fusion protein of anti-ROR1 and Buthus martensii Karsch recombinant analgesic anti-tumor polypeptide（IgG1-AGAP），and compare their effect on ovarian cancer cells. Methods：ROR1Fab vectors screened and preserved in our laboratory was used as template to construct ROR1-IgG1 and IgG1-AGAP eukaryotic expression vectors；supernatant of CHO-S cells after transfected by ROR1- IgG1 and IgG1-AGAP plasmids was collected and purified，respectively. Their immunological activity was identified and detected by ELISA，Biacore X100，fluorescence-activated cell sorting（FACS）and immunofluorescence. Cell counting kit-8（CCK-8），wound healing，Transwell invasion assays were used to analyze the effects of two antibodies on the ovarian cancer cell HO8910. The expression of relevant markers of epithelial mesenchymal transition（EMT） was detected by Western blot to evaluate the effects of ROR1-IgG1 and IgG1-AGAP on the migration and invasion of ovarian cancer cells. Results：ROR1-IgG1 and IgG1-AGAP were successfully prepared，the binding efficiency of IgG1-AGAP were similarly as ROR1-IgG1. Both IgG1-AGAP and ROR1-IgG1 inhibited proliferation，migration and invasion of ROR1-positive ovarian cancer cell HO8910. Compared with ROR1-IgG1，IgG1-AGAP can significantly inhibit the proliferation and migration of tumor cells（P < 0.05），and these effects were not observed in ROR1-negative cells IOSE386. Western blot results showed that the expression levels of Snail in ROR1-IgG1 group and IgG1-AGAP group decreased compared with the blank control group，while E-cadherin showed the opposite， suggesting that ROR1-IgG1 and IgG1-AGAP can regulate Snail and E-cadherin and inhibit EMT in ovarian cancer cell HO8910，thereby inhibiting their migration and invasion. Conclusion：Our findings demonstrate that IgG1-AGAP can target ROR1 expressing ovarian cancer cells effectively and have obvious antitumor activity. IgG1-AGAP could be as an appropriate and promising therapeutic strategy for ROR1-positive human cancers.

Abstract:Objective：This study amis to prepare the genetically engineered human-mouse chimeric antibody against hepatitis E virus（HEV）and investigate its protective neutralization and properties. Methods：To optimize the obtained anti-HEV murine monoclonal antibody gene sequence，the human-mouse chimeric engineered antibody gene sequences were designed and cloned into the antibody eukaryotic expression vectors；vectors were co-transfected into 293F cells for optimal expression，and the genetically engineered antibody was efficiently prepared. The binding ability and neutralizing activity of the engineered antibody was studied by enzyme linked immunosorbent assay（ELISA），immunofluorescence and HEV cell infection model. Results：The engineered human-mouse chimeric HEV protective antibody expression vectors were constructed successfully，and the engineered human-mouse chimeric HEV protective antibody was expressed in the 293F expression system efficiently. Antibody expression yield reached 30 mg/L，binding affinity of the antibody was 1.202×10-8 mol/L. Immunofluorescence results showed that the antibody could sensitively and specifically bind to Kernow cells infected by HEV. The results of quantitative real-time PCR showed that the antibody could protect susceptible C3A cells from HEV infection. Conclusion：Gene engineering antibody has high HEV binding capacity and efficient neutralization protection，which can effectively block HEV infection and be achieved with low-cost and high-efficiency.

Abstract:Objective：This study aims to prepare 68Ga-DOTA-K-PEG2-（K-FA）2（68Ga-DOTA-2P-FA2）and investigate the value of 68Ga-DOTA-2P-FA2 for targeted imaging of ovarian cancer and peritoneal metastasis. Methods：Characteristics of 68Ga-DOTA-2P-FA2 was investigated in vitro after its preparation. A nude mouse model of subcutaneous human ovarian cancer（SKOV3）and its peritoneal metastasis was established to investigate the biodistribution of 68Ga-DOTA-2P-FA2 in vivo. The receptor blocking group，the negative control group of human lung cancer（A549）and blank control group were set up. The microPET imaging was performed at 120 min（in mice bearing subcutaneous tumor xenograft）and microPET-CT imaging was performed at 60 min（in mice bearing peritoneal metastases）after tail vein administration. Results：68Ga-DOTA-2P-FA2 was stable in vitro with radiochemical purity of （96.30±1.28）%. Folate receptor affinity（IC50）was 17.1 nmol/L. Octanol/water partition coefficient（lg P）was -1.89. Radioactivity uptake of subcutaneous ovarian cancer reached a peak at 120 min after tail vein injection，which could be effectively blocked by overdose free folic acid. It could be removed rapidly in blood，heart and lung，generating a low uptake in liver，and mainly excreted through kidney. Pre-injection of pemetrexed could effectively accelerate the excretion of radioactivity in kidney. MicroPET imaging showed radioactivity uptake of subcutaneous ovarian cancer was significantly higher than that of folate receptor blocking group and that of the negative control group. The radioactivity uptake of peritoneal metastases was significantly higher than that of blank control detected by microPET-CT. Conclusion：68Ga-DOTA-2P-FA2，which accumulated in tumor sites with high expression of folate receptor，was a good imaging agent for ovarian cancer and peritoneal metastases.

Abstract:Objective：This study aims to investigate the expression of miR-1301 in hepatocellular carcinoma（HCC） tissues and cell lines，and explore the effect of miR-1301 on the proliferation of HCC cells and its underlying mechanism. Methods：The expression of miR-1301 in HCC tissues，adjacent tissues and cell lines was detected by RT-PCR. CCK-8，and colony formation assay were used to examine proliferation of HCC cells after transfection with miR-1301 mimic or inhibitor. The activity of PI3K，p-AKT and AKT was detected by Western blot. Results：The results showed that the expression of miR-1301 in HCC tissues and HCC cell lines was obviously down-regulated compared with that in the normal controls. RT-PCR showed that the expression of miR-1301 in HCC cells was significantly increased or decreased after miR-1301 mimic or inhibitor transfection（P＜0.01）. Compared with the control，knockdown of miR-1301 could remarkably inhibited the proliferation of Hep3B cells（P＜0.05），while overexpression of miR-1301 presented the opposite effects（P＜0.05）. Furthermore，we found that the activity of PI3K/AKT signal pathway was significantly inhibited by miR-1301. Conclusion：Expression of miR-1301 is low in HCC. The down-regulated miR-1301 in HCC inhibited the proliferation of HCC cells，the underlying mechanisms could be the inhibition of PI3K/AKT signal pathway by miR-1301．

Abstract:Objective：This Study aims to study the effect of silencing general control nonderepressible 5（GCN5）or SRY related HMG box-9（SOX9）gene on TGF-β1 production in the renal tissue of Thy-1 nephritis（Thy-1N） rats. Methods：The plasmids of GCN5 and SOX9 shRNA were packaged with lentiviral（LV）to form LV-shGCN5 and LV-shSOX9. Then，the LV-shGCN5 and LV-shSOX9 vectors were separately perfused into rat kidneys by renal artery perfusion，and next，the rat Thy-1N model was replicated by injection of rabbit anti-rat-thymocyte serum（ATS）through rat tail vein. Thereafter，rat renal tissues mentioned-above were taken at 3 h after ATS injection，and mRNA and protein levels of GCN5，SOX9 and TGF-β1 were examined by RT-PCR and Western blot to verify the effects of GCN5 or SOX9 gene interference on TGF-β1 expression in the renal tissues of rats with Thy-1N. Results：LV-shGCN5 or LV-shSOX9 vectors could be perfused successfully into rat kidneys through renal artery，and LV-shGCN5 or LV-shSOX9 not only effectively silenced GCN5 or SOX9 gene，but also downregulated TGF-β1 synthesis in the renal tissue of Thy-1N rats. Conclusion：Knockdown of GCN5 or SOX9 gene can significantly inhibit renal TGF-β1 production of rats with Thy-1N.

Abstract:Objective：This study aims to explore the regulatory role of transcription factor Krüppel-like factor 5（KLF5）in the proinflammatory factor［i.e. interleukin（IL）-36α］production in rat glomerular mesangial cells（GMC） stimulated by sublytic C5b-9. Methods：First，the changes of KLF5 or IL-36α mRNA and protein at different time were examined by reverse transcription PCR（RT-PCR）and Western blot in the GMC stimulated by sublytic C5b-9. Then，the plasmids of KLF5 overexpression（pIRES2-KLF5）and KLF5 short hairpin RNA（shKLF5）were generated and transfected rat GMC respectively，and the cells were treated with or without sublytic C5b-9. The effects of overexpressing KLF5 gene or silencing KLF5 gene on IL-36α synthesis in the GMC induced by sublytic C5b-9 were detected by RT-PCR and Western blot. Meanwhile，the activity of IL-36α promoter after KLF5 gene overexpression or knockdown in the GMC was measured by luciferase reporter assay. Results：The mRNA and protein expression of KLF5 and IL-36α were obviously elevated in the GMC stimulated by sublytic C5b-9，and the expression phase of KLF5 was earlier than that of IL-36α. KLF5 overexpression could promote IL-36α production，but KLF5 knockdown markedly decreased IL-36α expression in the GMC upon sublytic C5b-9 incubation. Sublytic C5b-9 treatment or KLF5 overexpression could significantly up-regulate IL-36α promoter activity，but KLF5 gene knockdown remarkably declined IL-36α promoter activity stimulated by sublytic C5b-9. Conclusion：The up-regulation of KLF5 has a promoting role in IL-36α synthesis in rat GMC stimulated by sublytic C5b-9.

Abstract:Objective：This study aims to investigate the effects of carboxypeptidase E（CPE） on the cell proliferation，cell cycle and apoptosis of mice glomerular mesangial cells（GMC）cultured in high glucose. Methods：The CPE gene fragment was amplified by PCR and inserted into the expression vector pcDNA3.1 plasmid to construct the recombinant plasmid pcDNA3.1-CPE. The plasmid was transfected into GMC，and the expression of CPE-tagged protein，proliferation marker CyclinD1，proliferating cell nuclear antigen（PCNA）and apoptosis marker Cleaved-caspase-3 were detected by Western blot；plate cloning，cell counting kit-8（CCK-8）and flow cytometry were used to detect the effect of overexpressed CPE on the proliferation，cell cycle and apoptosis of GMC cells cultured in high glucose. Results：The overexpression of CPE in GMC cells was successfully constructed. Plate cloning and CCK-8 showed that overexpression of CPE could inhibit the proliferation of GMC. Flow cytometry showed that the overexpression of CPE significantly promoted the cell apoptosis compared with the control group；the number of cells in G1 phase was significantly increased，while that in S phase was significantly decreased（P < 0.01）. Conclusion：CPE can inhibit the proliferation of mice GMC cultured in high glucose and induce cell apoptosis.

Abstract:Objective：This study aims to observe the effects of low-frequency electro-acupuncture（EA）auricular acupoint “stomach” on gastric antrum pathological morphology，interstitial cells of Cajal（ICC），and serum levels of ghrelin and vasoactive intestinal peptide（VIP）in streptozotocin（STZ）-induced diabetic rats. Methods：Tweenty-four male Sprague-Dawley（SD）rats were randomly divided into model group，sham auricular acupuncture group and auricular acupuncture group，8 rats in each group. All of the rats were injected with STZ（55 mg/kg）to induce diabetic model，at the end of the 8th week after injection，animals received 2 weeks-treatment（20 min per time，5 days a week，10 times）respectively：model group（no stimulation），sham auricular acupuncture group（2 Hz EA on earlobes），auricular acupuncture group（2 Hz EA on ear point”stomach”）. Serum level of Ghrelin and vasoactive intestinal peptide（VIP） were detected before and after EA treatments. At the end point of the experiment，pathological morphology in antrum was observed by hematoxylin-eosin staining. C-Kit expression of ICCs in antrum were analyzed by immunohistochemistry and Western blot. Results：Serum level of ghrelin in auricular acupuncture group was increased after EA treatments（P＜0.05）. Compared to model group，serum level of ghrelin in auricular acupuncture group was significantly higher at the 10th week after STZ injection（P＜0.05）. C-Kit expression of ICCs in antrum of auricular acupuncture group was higher than that in model group and sham auricular acupuncture group（P＜0.01）. Conclusion：Low-frequency EA on ear “stomach” point can promote the repair of ICCs in diabetic rats，which may be related to up-regulation of serum ghrelin.

Abstract:Objective：This study aims to investigate the relationship between gene polymorphisms of superoxide dismutase 2（SOD2）rs4880，glutathione S-transferase Pi（GSTP1）rs1695 and risk of cervical cancer. Methods： A case-control study was carried out including 395 patients with cervical cancer and 465 case-free controls during the same period. SOD2 rs4880 and GSTP1 rs1695 polymorphisms were examined by TaqMan-Probe assay method. Logistic regression model was used to calculate the odds ratio（OR）and 95% confidence interval（95% CI） between population with different genotypes. Results： When compared with SOD2 rs4880 TT genotype，the rs4880 TC，CC，and TC/CC genotypes might increase cervical cancer risk by 15.1%，96.6% and 18.7%，respectively，but the differences were not statistically significant（OR=1.15，95%CI：0.84~1.57，P=0.378；OR = 1.97，95% CI：0.64~6.09，P=0.231；OR=1.19，95%CI：0.87~1.61，P=0.271）；Compared with the GSTP1 rs1695 AA genotype，individuals with rs1695 AG，GG and AG/GG genotypes showed no significant changes in the risk of cervical cancer（P > 0.05）. Further meta-analysis on the relationship between the polymorphism of GSTP1 rs1695 and cervical cancer also suggested that there was no significant correlation between the genetic variation of rs1695 and the occurrence of cervical cancer. Conclusion：The polymorphisms of SOD2 rs4880 and GSTP1 rs1695 may not be related to the risk of cervical cancer.

Abstract:Objective：This study aims to investigate the expression of LAPTM4B-35 protein in normal salivary gland and salivary adenoid cystic carcinoma，and to explore the correlation between LAPTM4B-35 protein expression and clinicopathological characteristics of tumor patients. Methods：The clinical and pathological data of 95 cases of adenoid cystic carcinoma and 20 cases of normal salivary gland were collected. By immunohistochemistry analysis，LAPTM4B-35 expression was evaluated in salivary adenoid cystic carcinoma tissues and normal salivary tissues. The correlation of LAPTM4B-35 expression with clinic-pathological parameters was assessed using Chi-square or Fisher’s exact test and Logistic regression analysis. Results：The expression level of LAPTM4B-35 was variant among different cell types of normal salivary glands. It was expressed fairly low in serous and mucous acini，weak in intercalated duct and excretory duct cells，moderate in secretory/striated ducts. In 48.00% of high grade salivary adenoid cystic carcinoma tested，LAPTM4B-35 was high expression，which was significantly higher than that in low grade salivary adenoid cystic carcinoma. The expression rate of LAPTM4B-35 were significantly associated with histological grades and clinical stage. Conclusion：LAPTM4B-35 protein was differentially expressed in normal salivary gland cells，and expression of LAPTM4B-35 protein was significantly related to the histological grade and clinical stage of adenoid cystic carcinoma.

Abstract:Objective：This study aims to investigate the significance of lung lobe quantitative CT parameters in assessment of chronic obstructive pulmonary disease（COPD）. Methods：Total 102 patients with stable COPD were recruited，and performed with chest CT scan and pulmonary function tests. The indexes of pulmonary function include forced expiratory volume（FVC） and predicted value percentage（FVC%pred），forced expiratory volume in one second（FEV1） and predicted value percentage（FEV1% pred），first second rate（FEV1 / FVC），maximum ventilation volume （MVV） and its percentage in the expected value（MVV% pred），maximum mid expiratory flow（MMEF）. The percentages of low attenuation area below -950 HU（LAA%）and airway dimensions in each lung lobe segments were measured quantitatively. Results：Parameters of pulmonary ventilation function were linear negatively correlated with airway indicators（P < 0.001）. When LAA% of left lower lobe was 13.5%，the sensitivity in prediction of MVV% pred <50% and FEV1%pred <50% was 66.2% and 75.0%，specificity was 88.4% and 81.0%，respectively. Wall thickness of 3.35 mm in left lower lobe had 60.5% sensitivity and 72.9% specificity in the prediction of FEV1 <50%. LAA% combined with airway variables could account for proportional changes in FEV1%pred of 42%，FEV1/FVC of 52%，MMEF of 31%，and MVV%pred of 35%，respectively. Conclusion：Quantitative CT parameters of different lung lobe can reflect deterioration of lung function in patients with COPD，and can be applied to predict the severity of airflow limitation.

Abstract:Objective：This study aims to investigate the clinical significance of Tim3 in differential diagnosis of aplastic anemia（AA）and myelodysplastic syndrome（MDS）. Methods：The proportion of CD4+Tim1+ and CD4+Tim3+ cells in peripheral blood mononuclear cells（PBMC） from 24 AA patients，20 MDS patients，and 17 normal controls were detected by flow cytometry. Real time qPCR was performed to examine Tim3 mRNA and Gal-9 mRNA levels in PBMC. Results：There was no difference of the proportion of CD4+Tim1+ cells in PBSC among normal control，AA，and MDS groups；The proportion of CD4+Tim3+ cells in MDS patients was significantly higher than that in patients from AA and control groups（P < 0.05）；The percentage of CD4+Tim3+ cells was significantly increased in high-risk MDS patients compared with that in low-or medium-risk MDS patients（P < 0.01）and healthy controls（P < 0.05）. There was no difference of Tim3 mRNA and Gal-9 mRNA levels in PBSC among normal control group，AA group，and MDS group. Conclusion：Tim3 in MDS patients is significantly higher than that in AA patients and is related to the risk of disease. These findings suggest that Tim3 might be involved in the pathogenesis of MDS and might be utilized as a marker to distinguish AA and MDS.

Abstract:Objective：This study aims to establish a rapid and simple method for prenatal diagnosis of single gene disorders related to skeletal dysplasia by PCR amplification using amniotic fluid. Methods：Thirty eight fetuses with bone dysplasia diagnosed by ultrasound from April 2013 to January 2018 were carried out amniocentesis combined with diagnosis of single gene disorders of achondroplasia，hypochondroplasia，thanatophoric dysplasia and osteogenesis imperfecta in First Affiliated Hospital of Nanjing Medical University. Amniotic fluid extracted from pregnant women was added to PCR buffer containing neutral salt and other substances without fetal cell culture or extraction of DNA. The pH value of buffer was controlled at 8-10. The target fragments of FGFR3 and COL1A2 genes was amplified by directly adding 8 μL of amniotic fluid to the reaction mixture of PCR amplification and followed by sequencing to perform prenatal diagnosis. The effect of components in reaction mixture on amplification efficiency in the method was investigated. Results：In 38 cases of fetal samples，3 cases of thanatophoric dysplasia（including 1 case of type Ⅰ and 2 cases of type Ⅱ），3 cases of achondroplasia and 1 case of hypochondroplasia were detected. The best amplification efficiency was obtained by using neutral salt buffer，0.20 U/μL Promega Taq DNA polymerase and 1.2 pmol/μL each primer. Conclusion：This method omits the two tedious steps，which were amniotic fluid cell culture and extraction of fetal DNA from amniotic fluid. Furthermore，amplification can be carried out directly without concentration of amniotic fluid. The method shortens the time of diagnosis，saves the cost of diagnosis，and further reduces the possible cross contamination of samples in PCR processes. It will be a promising，rapid，and efficient tool for prenatal gene diagnosis.

Abstract:Objective：This study aims to observe the calcification time of the midpalatal suture，evaluate the relationship between the calcification and age or gender by using cone-beam computed tomography，which can be used for individual assessment and guidance before rapid maxillary expansion in adults and adolescents. Methods：The CBCT images of 211 subjects（4.2-43.8 years old）were divided into five stages according to the degree of the midpalatal suture calcification. The relationship between the calcification degree and age or gender was evaluated. Results：Calcification degree of midpalatal suture increased with age. Complete calcification was first seen in a 10-year-old female，approximately 50% of patients aged 15 had calcified. However，some uncalcified midpalatal suture were still found in adult patients，and the latest was found in a 37-year-old woman. Calcification of the palatal suture was later in adolescent males than in adolescent females，but there was no difference between males and females before and after adolescence. Conclusion：Calcification of the midpalatal suture is associated with age，and continues from age 10 to adulthood. But in some adult patients，the midpalatal suture is not calcified. Calcification of the midpalatal suture is later in adolescent males than in adolescent females. It suggested that individual assessment is essential before rapid maxillary expansion.

Abstract:Objective：This study aims to study the clinical effect of tongue crib combined with occlusal pad appliance in the early treatment of primary anterior crossbite. Methods：There were 80 cases of anterior crossbite in deciduous teeth selected，40 cases in experimental group were treated with tongue crib combined with occlusal pad appliance，and 40 cases in control group were treated with traditional occlusal pad appliance. The changes of jaw，teeth，soft tissue and hyoid in the two groups before and after treatment were analyzed by cephalometric technique. Results：Compared with the control group，ANB，UI-SN，UL-SnPos increased in experimental group，Cm-Sn-UL decreased in experimental group，the tongue crib combined with occlusal pad appliance was effective in the treatment of malocclusion of the upper and lower teeth and the facial form of the patients significantly，H-RGn，H-S，H-FH increased，H-C3 decreased，and the tongue crib combined with occlusal pad appliance made the hyoid move backward and downward. Conclusion：Tongue crib combined with occlusal pad appliance can effectively treat deciduous crossbite，especially for the position of hyoid bone，and can be used in clinical practice.

Abstract:N6-methyladenine（m6A）is identified as the most abundant internal RNA modification in eukaryotic cells，which depends on proteins that are “writers”，“erasers” and “readers” of m6A. The m6A modification regulates gene expression and plays vital roles in many human diseases，such as cancer，nervous system disease and embryonal growth retardation. This field has become a new research hotspot in the field of epitranscriptomics. In this comprehensive review，we have described the roles played by m6A modification in the development of cancer. Generally，the m6A modification takes part in the self-renewal and differentiation of tumor stem cells，regulates tumor cell proliferation，and is related to chemoradiation resistance. Possible interventions targeting m6A are also discussed in this review，which may be advantageous for precise treatment of tumor.

Abstract:Vitamin D plays an important role in calcium absorption and bone metabolism. More and more studies have found that vitamin D is also involved in the modulation of infection，inflammatory and immune responses.Vitamin D also plays an important role in regulating a variety of cells. Vitamin D insufficiency and deficiency are common worldwide，which is more common in children with bronchial asthma. The pathophysiology of bronchial asthma is chronic inflammation，airway hyperresponsiveness and airway remodeling. Vitamin D may affect the occurrence and development process of asthma. This review summarized the correlation between vitamin D and the morbidity of asthma，the mechanisms of vitamin D affecting asthma，and the clinical effects of vitamin D as an adjuvant therapy for childhood asthma.