Abstract:Objective：This study aims to establish a co-culture system of matured stromal vascular fraction （SVF） with Hepa1-6 and study the effects on lipid metabolism of hepatocytes. Method：Primary SVF cells were isolated from mice subcutaneous fat and induced to matured SVF cells with differentiation medium. Matured SVF cells were co-cultured with Hepa1-6 by using Transwell system for 2 days. RT-PCR was applied to detect the expression of differentiation biomarker such as PPARγ and PGC-1α in SVF cells and lipid metabolism related genes such as CD36，FATP2 and GPAT1 in Hepa1-6. Cellular triglyceride level of co-cultured Hepa1-6 was detected by GPO Enzyme Method. Results：Isolated primary SVF cells were successfully differentiated to mature SVF cells with significantly large lipid droplets after treated with adipocytes differentiation medium for 6 days. The expression level of PPARγ and PGC-1α of SVF cells was significantly increased. The cellular triglyceride level of co-cultured Hepa1-6 was significantly higher than that of control group accompanied with increased expression level of GPAT1 and CD36. Conclusion：The co-cultured system of matured SVF cells with Hepa1-6 in Transwell insert has been successfully established. Co-culture with mature SVF cells induces lipid accumulation in Hepa1-6. This process may be maintained by the elevated expression level of CD36 and GPAT1，increasing fatty acid absorption and triglyceride synthesis.

Abstract:Objective：This study aims to Explore the effect of circadian clock gene of brain and muscle ARNT-like protein-1（Bmal1）regulated T-type calcium channel（TTCC）on circadian rhythm of chronic heart failure（CHF）ventricular arrhythmia（VA）and its possible mechanisms. Methods：The C57BL/6J mice CHF model was established by transverse aortic constriction（TAC） and mice in control group（CON） were treated with sham operation. Some TAC mice were randomly assigned to receive TTCC inhibitor mibefradil（MIB） for 2 weeks（CHF+MIB）. Cardiac function was assessed by echocardiographic and the expression level of TTCC was determined by immune histochemical（IHC）staining and Western blot（WB）. Ventricular arrhythmia（VA）was induced through programmed electrical stimulation（PES） in each group. The circadian rhythms of Bmal1 and TTCC were detected by WB and real-time quantitative reverse transcription polymerase chain reaction（qRT-PCR），and the molecular mechanism by which Bmal1 regulated TTCC was determined by chromatin immunoprecipitation（ChIP）. Results：Compared with CON group，CHF mice had significantly reduced cardiac systolic function，increased internal diameter accompanied by obvious cardiac hypertrophy. Significantly increased TTCC re-expression level in CHF mice myocardium was detected by IHC staining and WB. PES indicated that VA occurrence was significantly increased when comparing CHF mice with CON mice. To a certain extent，using MIB could reduce the occurrence of VA in CHF mice. In addition，VA occurrence appeared circadian rhythm in all three groups（CON，CHF and CHF+MIB）. WB and qRT-PCR results suggested that the expressions of Bmal1 and TTCC in the myocardium of CHF mice exhibited circadian rhythm and ChIP suggested that Bmal1 could directly bind to the E-box site of TTCC promoter to regulate TTCC. Conclusion：In CHF mice，TTCC channel is highly re-expressed and shows circadian rhythm，and MIB can reduce the occurrence of VA in CHF mice. Bmal1 can directly bind to the E-box site of TTCC promoter region to regulate the transcription of TTCC.

Abstract:Objective：This study aims to observe the effect of high fat diet on jejunal and ileal epithelial function in mice. Methods：A total of 40 male C57BL/6J mice aged 8 weeks were randomly divided into two groups and fed with normal chowdiet and high-fat diet respectively for 3 months. Jejunum and ileum tissues were collected from mice，and the morphological changes of villi and crypts in jejunum and ileum were detectedby H&E staining. PAS staining was used to detect the number of goblet cells. Crypt units were isolated for primary 3D organoid culture in vitro，and the percentage of organoid per crypt ratio and the number of crypt-domains per organoid were observed. The expression of apoptotic genes in jejunum and ileum were determined by PCR. Results：The villus length and goblet cell number of jejunum and ileum were not significantly affected by high fat diet，but the crypt depth was significantly shallower. The results of 3D organoid culture in vitro showed that the differentiation ability of jejunal epithelial stem cells decreased，but that of ileal stem cells increased significantly. And apoptosis of both kinds of epithelial cells was significantly reduced. Conclusion：High fat diet changed the function of jejunal and ileal epithelial cells in mice，and had different effects on the differentiation ability of jejunal and ileal epithelial stem cells.

Abstract:Objective：This study aims to investigate the mechanism of free fatty acids regulating renal injury through IRE1 / XBP1 pathway in hypertriglyceridemic pancreatitis. Methods：Total 48 male C57BL/6 mice were randomly divided into control group（CON group），severe acute pancreatitis group（SAP group），hypertriglyceridemic pancreatitis group（HTGP group） and CD36 inhibitor group（SSO group）. At 24h after corresponding treatment，blood samples，pancreas and kidney tissues were obtained for serological detection，pathological analysis and immunofluorescence staining. Results：Compared with SAP group，HTGP group had higher levels of triglyceride and free fatty acids，and the severity of pancreatitis and renal injury in HTGP group were more severe. In the renal tissues of HTGP group，the expression levels of IRE1 and XBP1 protein related to endoplasmic reticulum stress and CD36 protein which mediated free fatty acid uptake were higher than those in SAP group. Additionally，after administration of CD36 inhibitor in HTGP group，the pancreatic and renal injury，the levels of inflammatory factors and the expression levels of IRE1 and XBP1 in renal tissue decreased significantly in SSO group as compared to HTGP group. Conclusion：In hypertriglyceridemic pancreatitis，CD36 mediated free fatty acids may activate endoplasmic reticulum stress through IRE1 / XBP1 pathway to regulate related renal injury.

Abstract:Objective：This study aims to investigate the contribution of M1 long-term potentiation（LTP）-like plasticity to the improvement of cognitive function by high frequency repetitive transcranial magnetic stimulation at healthy rat’s head. Methods：A total of 24 rats were randomly divided into control group and rTMS group（n=12）. The cognitive function was detected by Morris water maze. The M1 plasticity was reflected by the motor-evoked potential（MEP） before and after rTMS on the primary cortex（M1），the protein expressions of NR1，NR2A and NR2B in the M1 region of rats after high frequency rTMS were detected by Western blot. Results：After treatment，the spatial episodic memory and M1 plasticity showed significant improvement versus the control group（P＜0.05）. High-frequency rTMS in the prefrontal cortex can enhance the plasticity of the M1 region，which is characterized by increased LTP-like plasticity and up-regulated NR1 and NR2A protein expression in the M1 region. And the changes of LTP-like plasticity in M1 region were positively correlated with spatial episodic memory ability （P＜0.05）. Conclusion：High-frequency rTMS could improve spatial episodic memory and cortical LTP-like plasticity in healthy rats，and the improvement in spatial contextual memory was positively correlated with motor cortical plasticity.

Abstract:Objective：This study aims to investigate the effect of yes associated protein 1（YAP1）on cisplatin（CDDP）sesitivity of osteosarcoma cells under hypoxia and its underlying mechanism. Methods：Flow cytometry and CCK-8 were used to detect the CDDP sensitivity of osteosarcoma cells. Western blot was used to detect the expression of hypoxia inducible factor-1α（HIF-1α），YAP1，signal transducer and activator of transcription 3（STAT3）and p-STAT3 protein in human osteosarcoma cell line MG-63 under normoxic and hypoxic conditions after transfection with HIF-1 α-siRNA，YAP1-siRNA and STAT3-siRNA. Results：YAP1 was highly expressed in MG-63 cells after hypoxia（P < 0.05）. Overexpression of YAP1 significantly reduced the sensitivity of MG-63 cells to CDDP under hypoxic condition（P < 0.01）. The apoptosis rate of osteosarcoma cells was significantly increased（P < 0.01）and the sensitivity of osteosarcoma cells to CDDP was increased（P < 0.01）under hypoxic condition after transfection with YAP1-siRNA. The expression of p-STAT3 in MG-63 cells was significantly down-regulated after inhibiting YAP1 expression（P < 0.01）. Silencing STAT3 significantly increased the sensitivity of MG-63 cells to CDDP under hypoxia（P < 0.01）. Silencing HIF-1α significantly inhibited hypoxia-induced up-regulation of YAP1（P < 0.01），and incresed the sensitivity of MG-63 cells to CDDP. Conclusion：Hypoxia up-regulates YAP1 in osteosarcoma cells in a HIF-1α dependent manner. YAP1 may promote CDDP resistance of osteosarcoma cells by activating STAT3.

Abstract:Objective：This study aims to synthesize multifunctional neuroprotective agent with antioxidant and γ-aminobutyric acid （GABA） activity and test their neuroprotective activity against stroke. Methods：The edaravone analogues were synthesized from phenols with distinct substitutes；the antioxidant activity was determined by 1，1-diphenyl-2-picryhydrazyl （DPPH） free radical scavenging activity；the GABA activity was evaluated by patch clamp，and the neuroprotective effect of the compounds was tested by MCAO model in rats. Results：The 3′，5′-Diisopropyl-4′-hydroxyedaravone（6a）and 3′-methyl-5′-tert-butyl-4′-hydroxy- edaravone（6b）were successfully synthesized，both compounds showed good in vitro antioxidant activity and stronger neuroprotection than edaravone on the MCAO model；electrophysiological experiments showed that compound 6b hod GABA enhancement activity at low concentration and direct GABAR agonistic activity at high concentration. Conclusion：Based on the molecular framework of edaravone，two new neuroprotecting agents with both antioxidant activity and GABA activity were successfully constructed.

Abstract:Objective：This study was to assess the value of optical coherence tomography（OCT）in guiding the intervention for acute coronary syndrome（ACS）verified by the non-invasive quantitative flow ratio（QFR），functionally. Methods：Patients who were intended to undergo percutaneous coronary intervention（PCI）and evaluated by OCT during operation were enrolled. Patients target lesions with a minimum luminal area（MLA）>2.6 mm2 by OCT and in whom OCT showed no features of plaque rupture or dissection received optimal medical therapy（OMT） and these patients were assigned to OCT-OMT group. The other patients received PCI treatment and were assigned to OCT-PCI group. Data measured by OCT between groups were compared. Functional indexes derived from lesions with high quality images were retrospectively analyzed and validated by QFR. Adverse clinical events between two groups at 1 year follow-up were collected and data statistical analyses were carried out subsequently. Results：A total of 120 ACS patients who were intended to undergo PCI and evaluated by OCT during operation with 146 lesions were enrolled. Forty-seven patients（39.2%）were assigned to the OCT-OMT group，and the other 73 patients（60.8%）were assigned to the OCT-PCI group. According to OCT analyses，the average MLA of OCT-OMT lesions was significantly higher than that of OCT-PCI lesions（3.74 mm2 vs. 2.41 mm2，P < 0.000 1），with area stenosis significantly lower（62.57% vs. 73.58%，P < 0.000 1）. According to QFR analyses，the average QFR value of OCT-OMT lesions was significantly higher than that of OCT-PCI lesions（0.89 vs. 0.75，P < 0.000 1），the “QFR≤0.80” rate of OCT-OMT lesions was significantly lower than that of OCT-PCI lesions（7.14% vs. 84.7% P < 0.000 1）. According to 1 year follow-up，the incidence of major adverse cardiac events（death，myocardial infarction，target vessel revascularization and rehospitalization for angina）and major bleeding events were low（7.5% and 0.8%）with no significant difference between two groups. However，the incidence of minor bleeding events in OCT-OMT group was significantly lower than OCT-PCI group（RR=0.12，P=0.04）. Conclusion：OCT is safe and efficient in guiding OMT for patients with ACS with the standard of MLA>2.6 mm2 and existing neither plaque rupture nor dissection assessed by OCT. QFR can supply functional evidence additionally. Modest consistency was observed in evaluating and guiding for ACS between QFR and OCT.

Abstract:Objective：This study aims to develop a novel roadmap of right hepatic vein（RHV） tributaries for surgical planning based on three-dimensional（3D） visualization. Methods：3D simulated anatomical liver resection（ALR） of healthy participants who underwent contrast-enhanced computed tomography（CECT） of the upper abdomen from July 2018 to June 2021 in the First Affiliated Hospital of Nanjing Medical University were retrospectively reviewed. Liver volume，distributions of RHV and its tributaries，incidence of the inferior right hepatic vein（IRHV） were estimated. Results：Total 336 eligible participants were assessed，with a median age of 59（48~67）years. The total liver volume was 1 255.65（1 071.43~1 450.078）mL，which was significantly larger in males than in females（P < 0.05）. The Person correlation analysis revealed a positive and weak correlation between the diameter of RHV and the liver volume（r=0.242，P < 0.05）. 1（1~2）IRHV was detected in 44.6% of volunteers. A negative and poor correlation was noted between the standardized diameter of RHV and the incidence of IRHV（r=-0.109，P < 0.05）. The diameter of RHV without IRHV was significantly larger than that of RHV with IRHV（P < 0.05），which were 7.95（6.51~10.40）mm and 9.33（7.37~11.23）mm，respectively. There were15.8% of the participants excluded，whose RHV was short and unable to be fully exposed on the transection plane. Virtual RHV-oriented ALR of 283 participants with normal RHV was assessed to develop the roadmap of RHV tributaries. Hepatic veins draining segment Ⅵ（V6）and segment Ⅶ（V7）were concentrated at 0.45（0.33~0.55）and 0.78（0.71~0.84）of the transection plane in virtual right posterior sectionectomy respectively，where the number of V6 was significantly more than that of V7（P < 0.05）. Hepatic veins draining segment Ⅴ（V5）and segment Ⅷ（V8）were concentrated at 0.41（0.32~0.50）and 0.78（0.70~0.86）of that in simulated left trisectionectomy respectively，where the number of V5 was significantly more than that of V8（P < 0.05）. The diameter of V6 was significantly larger than that of V5（P < 0.05）. Conclusion：Virtual roadmap of RHV tributaries is of great significance to guide precise anatomical liver resection.

Abstract:Objective：We aim to explore the feasibility and safety of the S2b+S3a combined subsegmentectomy for pulmonary nodules between S2 and S3 in the right upper lung. Methods：The clinical data of segmentectomy from 2015 to 2020 in our center was cdlected，the nodule characteristics and perioperative data of the patients received S2b+S3a combined resection was analyzed. Propensity score matching was used to select the right upper lobe（RUL）resection cases with comparable age，gender，and pathological nodule diameter. Results：A total of 46 cases of S2b+S3a resection and 46 matched cases of RUL resection were included. The pathologic malignancy of nodules in the RUL group was higher than that in the S2b+S3a group（P=0.037） and the TNM stage was more advanced（P=0.026）. The nodules of the RUL group were deeper than that in the S2b+S3a group（P=0.005）. The number of lymph node sampled stations and the total number of sampled lymph nodes in the RUL group were larger than those in the S2b+S3a group（P<0.001）. In terms of postoperative data，the drainage volume on the first postoperative day，the total drainage volume，and the duration of chest tube drainage of the S2b+S3a group were all smaller than those in the RUL group. There was no perioperative death in both groups and no postoperative recurrence or death at the follow-up endpoint. Conclusion：S2b+S3a combined subsegmentectomy is a safe and feasible method to manage pulmonary nodules such as early non-small cell lung cancer between S2 and S3 under rational planning.

Abstract:Objective：This stndy aims to investigate the expression and function of long non-coding RNA（lncRNA）TPTEP1 in lung adenocarcinoma. Methods：Eighty-six pairs of lung adenocarcinoma patients’ tumor and adjacent-tumor tissue samples，33 pairs of preoperative and postoperative plasma samples from lung adenocarcinoma patients were collected，and qRT-PCR was used to detect the expression of TPTEP1. The correlation between TPTEP1 levels and clinical features were analyzed. After using lentivirus to overexpress the expression of TPTEP1 in lung adenocarcinoma cell lines A549，CCK-8 and tranwell experiments were used to evaluate the proliferation，invasion and migration ability of lung adenocarcinoma cells. Results：The expression of TPTEP1 in lung adenocarcinoma tissues and cell lines was significantly lower than that in the control group，and its expression was significantly related to the tumor size，tumor differentiation，lymph metastasis and TNM stage of lung adenocarcinoma. Overexpression of TPTEP1 significantly inhibited the proliferation，invasion and migration of lung adenocarcinoma cells. Conclusion：The expression of TPTEP1 in lung adenocarcinoma tissue and preoperative plasma is significantly reduced，and it could inhibit the proliferation and metastasis of lung adenocarcinoma cells，which might provide a feasible potential target for the diagnosis and therapy of lung adenocarcinoma.

Abstract:Objective：This study aims to study the relationship between thyroid hormone synthesis and metabolism-related gene expression and browning of visceral fat. Methods：The visceral fat tissues of normal weight and obese persons with normal thyroid function were collected，and the mRNA expression of genes related to thyroid hormone synthesis and metabolism，visceral fat beige functional protein and transcription factors were detected by fluorescence real-time quantitative PCR，then the correlation analysis was performed. Results：The mRNA expression of thyroid hormone transporters included solute carrier family 16 members 2 and 10（SLC16A2，SLC16A10），solute carrier organic anion transporter family member 1C1（SLCO1C1） thyroid stimulating hormone β subunit（TSHB）and iodothyronine deiodinase 2（DIO2）decreased significantly in the visceral adipose tissue of obese people. There were significant differences in SLC16A2，TSHB and DIO2 between two groups The expression of visceral fat beige functional protein UCP1 and marker transcription factor PR domain-containing 16（PR domain-containing 16，PRDM16）are positively correlated with the expression of the above genes after adjusted by age，gender，body mass indexand FT3. Conclusion：Decreased beige function of visceral fat in obese people is closely related to thyroid hormone synthesis and metabolism.

Abstract:Objective：This study aims to discover the characteristics of gene mutation and gene expression profile of primary adenoid cystic carcinoma（ACC） and mucoepidermoid carcinoma（MEC） in the lungs by sequencing. Methods：Total 14 cases of lung ACC and 24 cases of lung MEC were enrolled. Samples of tumor tissues and adjacent tissues were collected. After extracting DNA and RNA，DNA and RNA transcription group were used to gene sequencing and bioinformatics analysis. Results：Among the detected DNA mutations in cancer tissues，MYB/MYBL1 fusion was detected in 78.5%（11/14） lung ACC patients. The most common mutation in lung MEC patients was MAML2 rearrangement，and 3 MEC patients had EWSR1 fusion，and 1 case ROS1 fusion occurs in MEC patients. Among the RNA differential genes in cancer tissues and adjacent tissues，SOX10 and BCL11A are highly expressed in lung ACC，and the most differentially expressed gene in lung MEC is TFAP2A. Conclusion：SOX10 and BCL11A may become new biomarkers of lung ACC. TFAP2A is related to lung MEC resistance to anlotinib.

Abstract:Objective：This study aims to investigate the association of red blood cell distribution width（RDW）to the treatment response in the newly diagnosed patients with chronic myeloid leukemia（CML）after 3 months，6months and 12 months of tyrosine kinase inhibitor（TKI）treatment. Methods：A retrospective analysis of 175 newly diagnosed CML chronic phase patients who were treated in the First Affiliated Hospital of Nanjing Medical University from October 2010 to August 2021，all patients were treated with first-generation TKI（imatinib）or secend-generation TKI（nilotinib，dasatinib and flumatinib）. According to the treatment response，they were divided into two groups：the optimal response group and the warning or treatment failure group，to analyze the influence of RDW and related clinical data on treatment response. The receiver operating characteristic（ROC）curve was used to optimize the cut-off values of clinical indicator. Results：Patients with RDW ≥ 16.15% at first diagnosed had significantly worse response（warnings and failures）to treatment at 3 and 6 months than those with lower RDW（P＜0.05）. Analyzing the patients who did not achieve the best response after 3 months of TKI treatment and did not change the treatment early，combined with the RDW at the first diagnosed，it was found that the treatment failure rate of the high RDW group at 6 or 12 months was significantly higher than that of the low RDW group（P＜0.05）. Conclusion：RDW at the first diagnosis of CML-CP patients is an independent predictor for early treatment response（3 and 6 months）of TKI. Patients with high RDW and warning for the 3-month efficacy evaluation are more likely to fail treatment at 6 and 12 months

Abstract:Objective：This study aims to investigate the technical nuances and clinical effects of intraoperative ultrasonic-guided resection of deep-seated intracranial cavernous malformation. Methods：The clinical data of 45 patients with cavernous vascular malformation were retrospectively analyzed and divided into the ultrasound-guided group（26 cases） and control group（19 cases） according to whether ultrasound guidance was used during operation. General information and ultrasound images were recorded. The diameter and depth of each case，operation time under the microscope，and hospitalization period of the two groups were compared. KPS scores were compared between the two groups before surgery，one week after surgery，and three months after surgery，respectively. Results：Deep-seated cavernous malformations and surrounding structures had typical ultrasonic imaging characteristics. The two groups had a similar clinical backgrounds in diameter，depth，and preoperative KPS score（P > 0.05）. Compared with the control group，the operation time under the microscope（41.69±8.04）min， hospitalization period （6.92±1.57）d， and KPS score（65.38±7.60） one week after operation in the ultrasound group were significantly decreased［（50.74±8.52）min，（8.68±2.89）d，（58.42±10.68）分，P < 0.05］. However，there was no statistical difference in KPS scores three months after surgery between the two groups［（88.46±8.34） vs. （87.36±12.84）P > 0.05］. Conclusion：Ultrasound can help to reach the lesion quickly and accurately，thus shortening the operation time and hospitalization period，improving the surgical efficacy，and promoting the rapid recovery of patients.

Abstract:Objective：This study aims to investigate the relationship between hypoperfusion distal to anterior circulation in symptomatic intracranial atherosclerotic disease（ICAD）patients and recurrent ischemic stroke. Methods：One hundred and thirty symptomatic patients caused by middle cerebral artery stenosis were retrospectively analyzed. They were divided into initial group，with the first episode of acute and subacute ischemic stroke/transient ischemic attack（TIA）（n=92）；and recurrent group，with recurrent acute ischemic stroke/TIA（n=38）. Hypoperfusion was assessed quantitatively based on time threshold of Tmax>4 s and Tmax>6 s. The degree of stenosis，border zone infarction（BZI）and hypoperfusion volume of Tmax>6 s and Tmax>4 s were measured and compared between groups using independent sample t test，non-parametric test，chi-square or fisher’s exact test as appropriate. Logistic regression model was constructed to investigate the risk factors for recurrent stroke. Receiver operating characteristic curve analysis was used to determine the diagnostic performance of the parameters. Results：Higher degree of stenosis（P=0.003），more BZI pattern（P=0.037）and higher hypoperfusion volume defined by both Tmax>4 s and Tmax>6 s（both P＜0.001）were found in recurrent group compared to initial group. Multivariable logistic regression showed that hypoperfusion volume defined by Tmax>4 s was the only independent imaging marker that associated with stroke recurrence（OR=1.120，per 10 mL increase，95% CI：1.051~1.194，P < 0.001）. The area under the ROC curve was 0.756（95%CI：0.672~0.839），with a sensitivity of 76.3% and a specificity of 63.0%. Conclusion：Higher volume of distal hypoperfusion defined by Tmax>4 s was independently associated with recurrent ischemic stroke，and thus may be a useful imaging marker for stratifying the risk of stroke recurrence in symptomatic ICAD patients.

Abstract:Objective：To evaluate the ventricular systolic function of fetuses with gestational diabetes mellitus（GDM）by using 24-segment two-dimensional speckle tracking imaging（2D-STI）technique. Methods：A total of 111 singleton pregnant women in Changzhou second people’s Hospital affiliated to Nanjing Medical University from May 2020 to August 2021 were collected and divided into GDM group and normal control group according to the results of oral glucose tolerance test（OGTT）. All pregnant women underwent fetal echocardiography to obtain routine fetal echocardiographic evaluation indexes，including left ventricular ejection fraction（LVEF），left ventricular cardiac output（LVCO），area change fraction（FAC），cardiac globular sphericity index（GSI），mitral annulus plane systolic displacement（MAPSE），tricuspid annulus plane systolic displacement（TAPSE）. At the same time，24-segment 2D-STI technique was used to obtain fetal ventricular strain indexes：left ventricular global longitudinal strain（LVGLS），left ventricular global circumferential strain（LVGCS），right ventricular global longitudinal strain（RVGLS），right ventricular free wall strain（RVFWSt）. Results：There was no significant difference in traditional echocardiographic evaluation indexes and LVGLS between the GDM group and the normal control group，but the fetal LVGCS，RVGLS and RVFWSt in the GDM group were significantly lower than those in the normal control group（P＜0.05）. There were positive correlations between LVGLS and LVEF，LVFAC（r=0.544，0.714，P＜0.05），between LVGCS and LVEF，LVFAC，MAPSE（r=0.837，0.751，0.191，P＜0.05），and between RVGLS，RVFWSt and RVFAC（r=0.517，0.421，P＜0.05）. Maternal body mass index（BMI）was an independent predictor of RVGLS（β=0.496，95%CI：0.147~0.846，P＜0.05）. Conclusion：GDM can cause the decrease of fetal ventricular systolic function. 24-segment 2D-STI technique can monitor fetal ventricular systolic function timely and accurately，which is beneficial to clinical pregnancy monitoring.

Abstract:Objective：To reveal the serum，biotype distribution and molecular characteristics of isolates from different hosts in Jiangsu Province，2016-2019. Methods：Different kinds of specimens were collected for isolation strains，the isolated strains of Yersinia enterocolitic were evaluated by serotyping and biotyping. The five virulence genes（ail，virF，yadA，ystA and ystB）and multilocus sequence typing were analysed by using next-generation sequencing technology. Results：In this study，6 398 specimens were collected，and 186 strains of Yersinia enterocolitic were isolated. The serotype of 109 strains could not be identified，the dominant serotype that can be identified are O：8 and O：3. There are 3 biotypes of all isolates，most of which are biotype 1A. Based on sequencing analysis，type Ⅲ and type Ⅳ are the mainly virulence gene type in this area. All strains were divided into 81 STs by MLST. Conclusion：The serotype of Yersinia enterocolitic isolated from different hosts are complex，the biotype is relatively concentrated. Virulence gene type Ⅳ strains can be isolated from diarrhea patients. ST genotypes are distributed divergently. The hosts of pig，chick and dog should be supervision and surveillance.

Abstract:Objective：To estimate the health life expectancy（HALE）of residents aged 18 years and over in Suzhou，and to provide scientific basis for the establishment of health assessment system and the development of accurate prevention and control in Suzhou. Methods：A total of 6 208 adults aged 18 and above were chosen by multi-stage stratified random cluster sampling in 2019 to gather self-reported health data. Life expectancy table was based on annual official population and mortality data. Sullivan’s method and HOPIT model were used to calculate HALE（healthy life expectancy）. Results：In 2019，the HALE of the 18-year-old adults in Suzhou is 36.30 years，and that of the 60-year-old group is 7.80 years. The percentage of life lost is 45.17% and 69.56% respectively. With the increase of age，the percentage of life loss increases gradually. The loss of HALE and the percentage of life lost due to disability in all age groups of men were lower than those of women，and the difference increases with age. Conclusion：The health level of female adults in Suzhou is lower than that of men，and the health problems of the elderly have a major impact on the quality of the rest of their lives.

Abstract:Asthma is a heterogeneous disease characterized by chronic airway inflammation. Eosinophilic asthma is the most common subtype based on airway inflammatory classification. As alternative biomarkers for induced sputum，blood eosinophil，fractional exhaled nitric oxide，and serum periostin have been widely used in the study of eosinophilic asthma，but their ability to predict eosinophilic airway inflammation is controversial. Combined use of two or more biomarkers may improve the accuracy of diagnosis. In addition，with the development of omics technology and the in-depth understanding of the pathogenesis of asthma，new biomarkers are expected to be applied in clinic in the future.

Abstract:The prevalence of thyroid nodules has been increasing in recent years，and some studies have shown that glucose metabolic disorder may be one of the important reasons，mainly involving insulin/insulin-like growth factor 1，leptin，inflammation，genetic immunity and other mechanisms. Metformin can inhibit thyroid nodules through different signaling pathways. This article introduces the linkage between thyroid nodules and glucose metabolism disorders，and analyzes the mechanism of metformin inhibiting thyroid nodules.

Abstract:Lungcancer and pulmonary tuberculosis are two common diseases in respiratory system，which are correlated in pathogenesis. Scars，chronic inflammation and abnormal immunity in lung induced by tuberculosis increase the risk of lung cancer.The immunosuppressive status in lung cancer tumor microenvironment enhances immune evasion of Mycobacterium tuberculosis. Additionally，immunotherapy in lung cancer in fluences the development of tuberculous. Therefore，the main challenge in clinic practice is early diagnosis and therapeutics for lung cancer with pulmonary tuberculosis. Understanding the pathogenesis of lung cancer with pulmonary tuberculosis is helpful to develop new diagnostic and therapeutical methods. This article summarizes the pathogenesis，diagnosis and treatment of lung cancer with pulmonary tuberculosis for itsearly diagnosis and precision therapy.

Abstract:At present，long noncoding RNA（lncRNA） have become a new target for the treatment of cerebral ischemia-reperfusion injury. Accumulating evidence demonstrates that the lncRNA can participate in the regulation of cerebral ischemia-reperfusion injury by affecting neuronal cell autophagy. This article aims to systematically review the effects of lncRNA-mediated neuronal cell autophagy in brain deficiency from the perspective of autophagy，using “lncRNA”，“neuronal autophagy” and “cerebral ischemia-reperfusion injury” as keywords. Studies have shown that lncRNA can regulate important target genes at all stages of autophagy（induction，nucleation，extension，maturation，and autophagosome lysis），thereby regulating the level of autophagy and exerting neuroprotective effects. Understanding the autophagy regulation of lncRNA and its mechanism of action in cerebral ischemia-reperfusion injury is expected to bring new hope for the treatment of ischemic brain injury.