Abstract:Objective：To obtain Dabie bandavirus（DBV）specific antibody sequences from peripheral blood mononuclear cell （PBMC）of recovery patients with severe fever with thrombocytopenia syndrome（SFTS）. Then fully human antibodies against DBV were prepared. Methods：Enzyme - linked immunosorbent assay（ELISA）was used to detect the plasma antibody of SFTS recovery patients. PBMC samples from the recovery patients were selected for magnetic bead enrichment, and the specific single B cells with DBV glycoprotein（DBV-Gn）protein binding activity were obtained by flow cytometry. The antibody sequences were obtained by B cell receptor sequencing，and the prokaryotic expression of single chain antibody was performed. Finally，the expression，binding capacity， and neutralizing activity of the antibodies were identified by Western blot，ELISA，and neutralization experiment in vitro. Results：Total 25 blood samples from SFTS recovery patients were collected. The antibodies showed binding activity with DBV - Gn and DBV nucleocapsid protein（DBV -NP）were detected in all plasma. Six types of single chain antibodies were obtained after flow cytometry sorting and BCR sequencing analysis，all of which had good binding capacity and neutralizing activity with DBV. Conclusion：Six fully human antibodies with neutralizing activity to DBV have been successfully expressed.

Abstract:Objective：To elucidate the impact of small ubiquitin-related modifier（SUMO）modification on the protein levels and cellular localization of speckle type BTB/POZ protein（SPOP）and explore the correlation between sentrin-specific proteases 1 （SENP1）and SPOP in clear cell renal cell carcinoma（ccRCC）. Methods：Using wild type（WT）and Senp1 knockout murine embryonic fibroblasts（MEFs），we investigated the effects of Senp1 on Spop protein level and cellular localization. By comparing the protein expression levels of WT-Spop and its SUMO modification site mutants，the effects of SUMO modification on Spop protein levels were further confirmed. Proximity ligation assay（PLA）was employed to study the impact of Spop SUMOylation site mutation on the binding ability with small ubiquitin-related modifier 1（SUMO1）. Finally，the correlation between SENP1 and SPOP in ccRCC was examined utilizing datasets and ccRCC cell lines. Results：Senp1 knockout down-regulated the protein level and stability of Spop in MEFs without affecting its nuclear localization. Mutating the SUMOylation modification site of Spop attenuated its binding affinity with Sumo1，consequently leading to diminished protein levels. Notably，the expression of SENP1 and SPOP exhibited a positive correlation in ccRCC. Conclusion：Senp1 stabilizes Spop protein through deSUMOylation modification. The expression of SENP1 and SPOP is positively correlated in ccRCC.

Abstract:Objective：This study aims to investigate the expression of ubiquitin-binding enzyme E2T（UBE2T）in papillary thyroid carcinoma（PTC）tissues and its effect on patient prognoses. Additionally，the effects of UBE2T on PTC cell function were explored， aiming to identify potential regulatory pathways and provide theoretical foundations for future targeted therapies. Methods：By using The Cancer Genome Atlas（TCGA）database，the UBE2T expression in PTC tissues and its association with patient prognosis were systematically analyzed. The UBE2T expression in tumor tissues and adjacent normal tissues of thyroid were assessed by Western blot. UBE2T knockdown experiments were performed in PTC cell lines（TPC - 1 and KTC - 1）. Cell proliferation，migration，and invasion abilities were evaluated by using CCK -8 and colony formation，wound healing and Transwell assays，respectively，with Western blot measuring protein levels. Results：The TCGA analysis revealed significantly elevated UBE2T expression levels in PTC tissues， correlated with disease -free interval and lymph node metastasis（P ＜ 0.01）. The UBE2T knockdown led to decreased proliferation， migration，and invasion ability in TPC-1 and KTC-1 cells，accompanied by the reduced STAT phosphorylation levels. The proliferation of UBE2T - knockdown cells significantly increased when treated with a STAT activator. Conclusion：The UBE2T knockdown suppresses the proliferation，migration，and invasion of PTC cell lines，suggesting UBE2T as a potential therapeutic target for PTC by modulating the JAK-STAT signaling pathway.

Abstract:Objective：To identify genes associated with mRNA expression-based stemness index（mRNAsi）in gastric cancer（GC） and explore their functions. Methods：Bioinformatics analysis was conducted using The Cancer Genome Atlas（TCGA）database and microarray GSE66229 dataset. The expression of TCEAL7 in tissues and cell lines was assessed by quantitative real-time polymerase chain reaction（qRT-PCR）and immunohistochemistry. The effect of TCEAL7 overexpression on the proliferation，migration，invasion， and stemness characteristics of GC cells was studied. Results：The expression of TCEAL7 was downregulated in GC. High expression of TCEAL7 was associated with poorer prognosis. Overexpression of TCEAL7 inhibited the proliferation，migration and invasion of GC cells，attenuated spheroid formation，and decreased the population of stem-like CD44^high/CD24^high cells. The nomogram which included clinical features and TCEAL7 expression showed good accuracy and discrimination for predicting the prognosis of patients with GC. Conclusions：TCEAL7 was identified as a mRNAsi - related prognostic factor in GC，inhibiting the malignancy of tumor cells and attenuating stemness characteristics.

Abstract:Objective：To explore the feasibility of constructing multimodal fused images of the temporomandibular joint area using the dilated residual attention network method，and to provide a feasibility analysis for improving the comprehensive diagnostic and therapeutic capabilities under multimodal fusion imaging of the oral temporomandibular joint. Methods：The dilated residual attention network was used to extract image features of MR and CBCT，and a“Softmax weighting strategy”to fuse the features. Subsequently，the corresponding images of the two modalities were fused together through an image reconstruction module. Results：The fused images could present the morphology of condylar cortical bone，condylar medullary bone，condylar attached muscles and articular disc. The fused images performed well in terms of peak signal -to - noise ratio and structural similarity index，with peak signal -to - noise ratio ranging from 10 to 15 and structural similarity index ranging from 0.4 to 0.6. Conclusion：This method can achieve real -time image fusion，the final fused image can reflect clear anatomical morphological features，thus avoiding the need for switching between multimodal images and providing effective guidance for dental experts in preoperative and postoperative clinical diagnosis.

Abstract:Objective：To investigate the clinical characteristics and pregnancy outcomes of women with uterine rupture. Methods： This retrospective study analyzed clinical data from 43 women who experienced uterine rupture during delivery at the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University from December 2016 to December 2022. The participants were divided into scarred and non-scarred uterus groups based on their histories of uterine surgery. Additionally，the delivery outcomes of 20 women who underwent vaginal trial of labor was analyzed. Results：Of the 43 cases，33 women involved a scarred uterus group，and 10 women involved a non-scarred uterus group. The non-scarred uterus group exhibited significantly higher intraoperative blood loss ≥ 1 000 mL（P ＜ 0.001）and cumulative blood loss ≥1 000 mL（P=0.003）compared to the scarred uterus group，along with a higher rate of blood transfusion（P=0.012）. Among those 43 cases，23 women underwent expected cesarean sections，while 20 opted for vaginal trial delivery. In the vaginal trial delivery patients，there was a notable increase in complete uterine ruptures（P=0.044）and rate of blood transfusion（P ＜ 0.001）in the group with uterine rupture detected during delivery（7 cases），compared with the group with uterine rupture detected after delivery（13 cases）. Furthermore，the group with uterine rupture detected during delivery showed a higher incidence of abnormal amniotic fluid and fetal heart rate changes. Although these differences did not reach statistical significance（P ＞ 0.05），they still warrant significant clinical attention. Conclusion：Non-scarred uterus exhibit more bleeding when rupture occurs. Patients undergoing vaginal trial delivery who experience postpartum uterine rupture face more severe complications. It is essential to closely monitor amniotic fluid，fetal heart rate，and vital signs before and after delivery to promptly detect uterine rupture and reduce adverse pregnancy outcomes.

Abstract:Objective：To explore the application value of the turbo spin echo（TSE）T2-weighted imaging based on deep learning reconstruction（DLR）technology in improving mediastinum image quality and reducing scanning time. Methods：35 patients with mediastinal lesions were prospectively collected for mediastinal conventional T2WI and DLR - based T2WI scanning. The artifacts， image clarity，display of the detail of the lesion and overall image quality of the two groups were qualitatively scored by two radiologists independently. The signal -to - noise ratio of the lesion（SNR_lesion）and the contrast to noise ratio of the lesion to vertical muscle（CNR_{lesion/muscle}）of the two groups of images were measured and compared. Results：Qualitative analysis：T2WI_DLR was superior to conventional T2WI in the scores of artifact，image sharpness，display of the detail of the lesion，and overall image quality（P ＜ 0.001）. Quantitative analysis：The SNR and CNR of the image of T2WI_DLR sequence were better than those of conventional T2WI（P ＜ 0.001）. Conclusion：Compared with conventional T2WI sequences，T2WI_DLR sequence has fewer motion artifacts，better image clarity，lesion detail display and overall image quality，and higher SNR and CNR，which has great potential in clinical application of mediastinal diseases.

Abstract:Objective：This study investigated the expression of mitochondrial autophagy - related genes（MRG）in asthma to establish a novel model for disease prediction，and also identified asthma subtypes based on the MRG to figure out the potential molecular targeted drugs. Methods：The data of asthmatic airway samples were obtained from gene expression omnibus data base. Differentially expressed MRGs were screened and validated in asthmatic mice or primary airway epithelial cells challenged by interleukin（IL）- 13 with immunohistochemistry so as to build a model for disease prediction using machine learning algorithms. According to the different MRG expression pattern，two subtypes of asthma were defined，and biological functions and signaling pathways were investigated by gene ontology（GO）and kyoto encyclopedia of genes and genomes（KEGG）analysis to find out the potential agents through a connectivity map database. Results：MRG expression in asthma patients was significantly increased compared with those in healthy subjects. Among these genes，translocase of outer mitochondrial membrane 5（TOMM5）was found to be the top differentially expressed MRG，which were up-regulated both in the airway epithelium of asthma patients or asthmatic mice and the primary airway epithelial cells stimulated by IL-13. In 22 MRGs，seven genes[TOMM5，FUN14 domain containing 1，translocase of outer mitochondrial membrane 22，sequestosome 1，phosphoglycerate mutase 5，mitofusin-2，ribosomal protein S27a]were screened to establish a model for disease prediction for its good performance exhibited by a receiver operating characteristic curve assessment in asthma. Through a consensus cluster analysis，two subtypes of asthma were classified considering the differences of gene expression and pathway enrichment. The predicted small molecule agents targeting these two subtypes were XMD8 - 92 and Verrucarin - A，respectively. Conclusion：Seven MRGs were confirmed to be the effective molecular markers for asthma prediction，and our findings may provide valuable evidences and open a new insight for the development of individualized approaches for asthma management.

Abstract:Objective：To explore the correlation between magnetic resonance（MR）findings[optic nerve sheath diameter（ONSD） and peripheral cerebrospinal fluid volume/intracranial volume ratio（PCSFV/ICV）]and cerebrospinal fluid pressure（CSFP）in patients with leptomeningeal metastases（LM）from non - small cell lung cancer（NSCLC）. And to assess the diagnostic accuracy of combined ONSD and PCSFV/ICV in diagnosing elevated CSFP. Methods：A total of 63 patients with NSCLC-LM were enrolled. Both optic nerve and cranial MR scans were performed to measure bilateral ONSD，cranial PCSFV and ICV. Subsequently，CSFP was measured in all patients through lumbar puncture，and intrathecal chemotherapy was administered regularly. Pearson correlation analysis was used to analyze the correlation between CSFP and MR findings. The accuracy of combined ONSD and PCSFV/ICV in diagnosing elevated CSFP was evaluated through receiver operating characteristic（ROC）curve analysis. Results：In patients with NSCLC-LM，the average ONSD of bilateral eyes was positively correlated with CSFP（r=0.567，P ＜ 0.001）. PCSFV/ICV was negatively correlated with CSFP （r=-0.365，P=0.003）. ROC curve analysis showed that the performance of ONSD，PCSFV/ICV，and combined ONSD with PCSFV/ICV in diagnosing intracranial hypertension（CSFP＞15 mmHg）were 0.81（95%CI：0.71-0.91），0.76（95%CI：0.64-0.89），and 0.91（95%CI：0.84-0.99），respectively. The performance of ONSD，PCSFV/ICV，and combined ONSD with PCSFV/ICV in diagnosing intracranial hypertension（CSFP＞20 mmHg）were 0.70（95%CI：0.58-0.82），0.72（95%CI：0.57-0.88），and 0.81（95%CI：0.70-0.93），respectively. Conclusion：The utilization of MR for measuring ONSD and PCSFV/ICV provides a valuable means to assess variations in CSFP among patients diagnosed with NSCLC -LM. Additionally，the combination of these two measurements enhances the diagnostic accuracy for evaluating CSFP，demonstrating its potential clinical significance.

Abstract:Objective：To compare the accuracy of end - tidal carbon dioxide partial pressure（P_ETCO₂）and transcutaneous carbon dioxide partial pressure（P_TCCO₂）in predicting arterial carbon dioxide pressure（P_aCO₂）in patients undergoing retroperitoneoscopic urologic surgery. Methods：Fifty patients undergoing retroperitoneoscopic urologic surgery under general anesthesia were included. Values of P_aCO₂，P_ETCO₂，and P_TCCO₂ were measured before and 30，60，90 min after insufflation. The differences between P_aCO₂-P_ETCO₂ and P_aCO₂-P_TCCO₂ were calculated. Correlation and regression analysis were conducted between P_aCO₂ and P_ETCO₂，as well as between P_aCO₂ and P_TCCO₂. Bland-Altman analysis was used to assess the agreement between P_aCO₂ and the other two variables. Results：The absolute differences of P_aCO₂-P_ETCO₂ and P_aCO₂-P_TCCO₂were（13.20 ± 4.43）mmHg and（4.35 ± 2.56）mmHg，respectively（P ＜ 0.05）. The correlation coefficient between P_aCO₂ and P_ETCO₂was 0.79（r ² =0.62，P ＜ 0.001），and between P_aCO₂ and P_TCCO₂ was 0.91（r ² =0.83， P ＜ 0.001）. The 95% limits of agreement between P_aCO₂ and P_ETCO₂ were 4.53 to 21.88 mmHg and between P_aCO₂ and P_TCCO₂ were -3.18 to 10.48 mmHg. Conclusion：P_TCCO₂ monitoring improves the accuracy of estimating P_aCO₂ in patients undergoing retroperitoneoscopic urologic surgery.

Abstract:Objective：To assess the effectiveness and safety of combined treatment with tranexamic acid（TXA）and erythropoietin （EPO）during the surgery for intertrochanteric fracture（IF）. Methods：A total of 174 patients with IF underwent surgery admitted to the Affiliated Jiangning Hospital of Nanjing Medical University from January 2018 to January 2023 were enrolled in this study. The patients were randomly divided into 3 groups：control group（Group A，n=62），TXA alone group（Group B，n=57），and TXA combined with EPO group（Group C，n=55）. Perioperative total blood loss，obvious blood loss and hidden blood loss in the three groups were calculated separately. The blood transfusion rate and blood transfusion volume，haemoglobin（Hb）levels，hospitalization day，and complications such as thrombosis were recorded. Results：There was no significant difference in Hb levels between three groups on admission. The Hb levels in Group C were significantly improved compared to the other two groups before surgery，2 and 5 days after surgery（P ＜ 0.05）. The total blood loss，transfusion rate，and transfusion volume in Group C were all lower than those in Group A，and the differences were statistically significant（P ＜ 0.05）. There was no statistically significant difference in the incidence of thrombosis and other complications among three groups（P ＞ 0.05）. Conclusion：The combined use of TXA and EPO intravenously and locally during surgery significantly reduces perioperative blood loss and blood transfusion rate，shortens hospital stays，lowers hospitalization costs，and does not increase the risk of complications such as thrombosis，in patients with IF.

Abstract:Objective：To investigate the clinical value of small pulmonary vessels parameters measured by chest CT in evaluating the classification of different types of pulmonary hypertension（PH）and the severity grading of connective tissue disease s - related pulmonary hypertension（CTD-PH）. Methods：A retrospective analysis included 170 PH patients，including 60 CTD-PH patients，52 idiopathic PH（IPAH）patients，and 58 chronic obstructive pulmonary disease - related PH（COPD - PH）patients，with 120 healthy controls（HC）included as controls. The ratio of the sum of the cross-sectional area（CSA）of small pulmonary vessels with CSA ＜5 mm² （%CSA_＜5）and between 5 to 10 mm²（ %CSA_{5- 10}）to the total CSA of the lung measured by chest CT was compared among the four groups using one-way ANOVA or Kruskal-Wallis test，followed by pairwise comparisons. Receiver operating characteristic（ROC）curve analysis was used to evaluate the performance of %CSA for differentiating mild to moderate CTD-PH（CTD-LM-PH）from severe CTD-PH（CTD-S-PH）patients，and calculate the area under the curve（AUC），sensitivity and specificity. Results：The %CSA_＜5 of the IPAH and COPD -PH groups were significantly lower compared to the HC group（P ＜ 0.001）. Additionally，the %CSA_{5 - 10} of the COPD -PH group showed a significant decline compared to the HC group（P=0.038），whereas the %CSA5-10 of the CTD-PH and IPAH groups was significantly high compared to the HC group（both P ＜ 0.05）. In comparisons between different types of PH groups，the %CSA_＜5 and % CSA_{5 - 10} of the CTD - PH group were higher than those of the COPD - PH group（P ＜ 0.001）. The % CSA_{5 - 10} of the IPAH group was significantly higher than that of the CTD -PH group（P=0.022），while there was no significant difference in the %CSA_＜5 between the IPAH and COPD-PH groups（P=0.833）. The %CSA_＜5 of CTD-S-PH group was significantly lower than that of CTD-LM-PH group（P= 0.004）. The ROC curve analysis showed that the optimal cutoff value for %CSA_＜5 to predict CTD -S -PH was 0.804，with an AUC of 0.710（95%CI：0.573-0.847），sensitivity of 0.714 and specificity of 0.320. Conclusion：The quantitative parameter %CSA assessed by chest CT can distinguish different types of PH. The %CSA_＜5 can serve as a reference for evaluating the severity of CTD-PH.

Abstract:Objective：To investigate the expression and significance of polymorphonuclear myeloid-derived suppressor cells（PMN-MDSC）in patients with hepatitis B virus - related acute -on -chronic liver failure（HBV -ACLF）. Methods：A total of 40 HBV -ACLF patients admitted to Yijishan Hospital of Wannan Medical College from September 2022 to August 2023 were selected as the study group，while 20 patients with chronic hepatitis B（CHB）and 10 healthy controls（HC）diagnosed or undergoing examinations at the same hospital during the same period were collected as the two control groups. Clinical data were collected and blood samples were obtained. Flow cytometry was used to detect the frequency of peripheral blood PMN-MDSC on the day of admission，and to compare the differences in the frequency of PMN-MDSC between the study group and the two control groups. Spearman’s correlation analysis was used to investigate the correlation between the frequency of PMN -MDSC and inflammation markers or disease severity. HBV -ACLF patients were further grouped based on whether they had concurrent or secondary infections and the prognosis on day 28 of follow-up， and the differences in PMN -MDSC frequency among groups were compared. Results：The frequency of PMN -MDSC in HBV -ACLF group was significantly higher than that in the CHB group and the HC group（P ＜ 0.001 for both），whereas there was no significant difference in the PMN-MDSC frequency between the CHB and HC groups（P ＞ 0.05）. The frequency of PMN-MDSC in HBV-ACLF patients was positively correlated with the white blood cell count，neutrophil - to - lymphocyte ratio，procalcitonin，international normalized ratio，total bilirubin，Child -Turcotte -Pugh score，and the model of end - stage liver disease score（r=0.347，0.799，0.506， 0.450，0.462，0.470，0.481，respectively，all P ＜ 0.05），and negatively correlated with the lymphocyte count（r=-0.428，P ＜ 0.01）. Among the 40 HBV-ACLF patients，18 had concurrent infections，17 had secondary infections，and 21 had poor prognosis on day 28， with their peripheral blood PMN - MDSC frequencies significantly higher than those of the control group patients（all P ＜ 0.001）. Conclusion：PMN-MDSC are enriched in HBV-ACLF patients and the frequency of PMN-MDSC in peripheral blood is closely related to infection risk，disease severity，and short-term prognosis of patients.

Abstract:Objective：Combined ultrasound and axillary metastatic clipped lymph nodes was developed a model to predict the pathological complete response（pCR）of axillary lymph nodes in clinically lymph node - positive（cN +）breast cancer patients after neoadjuvant systemic therapy（NST）. Methods：Eighty-eight patients were randomly assigned to the testing or validation set at a ratio of 7∶3. Before NST，the lymph nodes most suspicious on ultrasound images and confirmed as metastatic by pathological biopsy were selected and marked with a titanium clip under ultrasound guidance. Univariate and multivariate logistic regression analyses of the testing set were performed. A risk score model was developed based on the results of multivariate analysis. Results：The axillary pCR rate was 48%（42/88）. Hormone receptor status，N grade and changes in the number of abnormal lymph nodes were determined by ultrasonography，and changes in cortical thickness of the clipped lymph nodes were identified as independent factors and established the risk score model. In the score range of-13 to-9 and 1 to 10，the axillary pCR rate of the testing set was 100% and 0% ， respectively. The area under the receiver operating characteristic curves of the testing and validation sets were 0.931（95% CI：0.868- 0.994）and 0.762（95% CI：0.576-0.947），respectively. Conclusion：The risk score model based on ultrasound and clipped lymph nodes accurately predicte the axillary lymph node status of breast cancer patients with cN + after NST. When the risk score was between -13 and -9，the false-negative rate of axillary lymph node metastasis was 0%，allowing these patients to avoid axillary lymph node dissection and a series of complications.

Abstract:Objective：The use of drains in total knee arthroplasty（TKA）remains controversial. This study aimed to investigate the effects of intra-articular drainage on blood loss and joint function recovery in TKA. Methods：A retrospective analysis was conducted on patients with end-stage knee osteoarthritis who underwent TKA at the First Affiliated Hospital of Nanjing Medical University from September 2021 to October 2022. Patients were divided into a drainage group and a non-drainage group according to whether drains were placed intraoperatively. Postoperative blood loss，knee joint functional recovery index，postoperative complications，and follow-up results were compared between the two groups. Results：A total of 147 patients were included，with 53 cases in the non-drainage group and 94 cases in the drainage group. Compared to the non-drainage group，the drainage group had significantly less hidden blood loss （HBL）[（906.94±438.41）mL vs.（ 1 262.85±563.46）mL，P ＜ 0.001]，but there was no significant difference in transfusion rate or total blood loss（P ＞ 0.05）. The degree of lower limb swelling in the non-drainage group was higher than that in the drainage group at postoperative day 1，3，and 5，with significant differences noted at postoperative day 3[（9.31±4.47）% vs.（ 7.74±3.75）%，P=0.025]. The drainage group showed a significant increase in postoperative range of motion（ROM）of the knee joint compared to the nondrainage group（P ＜ 0.001），along with a significant increase in postoperative pain at day 3（P=0.029）and a decrease in postoperative ecchymosis rate（52.13% vs. 79.25% ，P=0.001）. However，there was no statistically significant difference in the incidence of postoperative deep venous thrombosis（DVT）between the two groups（P=0.552）. Both groups achieved primary healing of the incision without signs of infection such as redness，discharge，or liquefaction of fat. No cases of drainage obstruction，continuous bleeding at the drainage site，or dislocation or rupture of the drainage tube were observed in the drainage group. There were no cases of infection during the follow-up period in either group，and there were no significant differences in knee joint function recovery and pain level at 1 and 3 months postoperatively（P ＞ 0.05）. Conclusion：Intra - articular drainage in TKA can reduce HBL and alleviate early postoperative pain and swelling.

Abstract:Objective：To evaluate the diagnostic value of peripheral blood microRNA（miR）-21 and plasma poly（ADP - ribose） polymerase-1（PARP-1）in allergic rhinitis（AR）and combined allergic rhinitis and asthma syndrome（CARAS）. Methods：Peripheral blood samples were collected from 44 CARAS patients，31 AR patients，and 42 healthy controls. The expression levels of miR-21 in peripheral blood were detected by RT-qPCR，and the plasma levels of PARP-1 protein were measured by ELISA. Correlation analysis was performed by rearson correlation analysis. The diagnostic sensitivity and specificity of miR- 21 and PARP-1 were determined by receiver operating characteristic（ROC）curve. Results：The expression of peripheral blood miR - 21 was high in CARAS patients compared with healthy controls. The level of PARP - 1 was higher in AR patients than that in CARAS patients and healthy controls. Pearson correlation analysis showed that the expiression of miR - 21 was correlated with eosinophils count in AR patients and with fractional nasal nitric oxide（FnNO）in CARAS patients. The plasma level of PARP-1 was correlated with forced expiratory volume in one second percent predicted（FEV₁%_pred）in CARAS patients and with FEV₁%_pred and forced expiratory volume in one second（FEV₁）/forced vital capacity（FVC）（FEV₁/FVC）in CARAS patients. ROC curve analysis showed that when peripheral blood miR-21 was used as a diagnostic marker for CARAS，the sensitivity was 51.35% and the specificity was 80.95%. When the plasma PARP -1 was used as a diagnostic marker for AR，the sensitivity was 90.32% and the specificity was 54.76%. When the plasma PARP-1 was used as a diagnostic marker for the progression from AR to CARAS，the sensitivity was 45.45% and the specificity was 90.32% . Conclusion：There are differential expressions of miR -21 and PARP -1 in peripheral blood of patients with AR and CARAS. the peripheral bolld miR -21 can serve as a diagnostic biomarker for CARAS，while the plasma PARP-1 can serve as a diagnostic biomarker for AR and as a biomarker for the progression from AR to CARAS. This has significant value in identifying diagnostic and therapeutic targets for AR and CARAS.

Abstract:Objective：To explore the risk factors for postoperative recurrence of atrial fibrillation after concomitant Maze Ⅳ ablation for cardiac surgery and construct a nomogram model for risk prediction. Methods：Data were collected from 596 patients who underwent concurrent cardiac surgery and Maze Ⅳ ablation at the Department of Cardiac Macrovascular Surgery of the First Affiliated Hospital of Nanjing Medical University，from January 2014 to December 2022. Patients were divided into two groups based on whether atrial fibrillation recurred within 1 year postoperatively. Univariate chi-square test，multivariate logistic regression，and Cox regression models were used to analyze the independent risk factors for atrial fibrillation recurrence within 1 year postoperatively and to construct a risk prediction model. Results：Among the 596 patients，150（25.2%）experienced recurrences. Univariate analysis showed that age， duration of atrial fibrillation，left atrium diameter（LAD），right atrium diameter（RAD），prior pacemaker placement，and early atrial fibrillation recurrence were risk factors for postoperative recurrence of atrial fibrillation，while multifactorial analysis showed that age， duration of atrial fibrillation，LAD，and early atrial fibrillation recurrence were independent risk factors. Cox regression analysis demonstrated that age[HR=1.035（1.025-1.068），P ＜ 0.001]，duration of atrial fibrillation（HR=1.003[1.001-1.006]，P=0.003），LAD [HR=1.025（1.006- 1.044），P=0.009]，use of a hinged ablation forceps[HR=3.269（2.083- 5.130]，P ＜ 0.001]，and early postoperative atrial fibrillation recurrence[HR=3.592（2.532-5.095），P ＜ 0.001]were significantly associated with atrial fibrillation recurrence. According to the receiver operating characteristic curve analysis，the cut-off values for age，duration of atrial fibrillation， and LAD were 59.5 years，9.5 months，and 51.5 cm，respectively. Conclusion：Maze Ⅳ has good long -term efficacy in maintaining sinus rhythm. In patients with atrial fibrillation undergoing cardiac surgery，older age，longer LAD，and longer duration of atrial fibrillation can adversely affect the ablation outcome. The hazard model constructed based on these results has good predictive performance.

Abstract:Parkinson’s disease is a common neurodegenerative disorder characterized by the degeneration and death of dopaminergic neurons in the substantia nigra of the midbrain. PET/MR is a novel imaging technology that has been developed in recent years to enable the simultaneous acquisition of metabolic and structural images. This technology holds great value in the early detection of clinical markers for Parkinson’s disease. Artificial intelligence models have been extensively employed in clinical settings to assist physicians in diagnosis，yielding promising outcomes. In this paper，we aim to review the application of machine learning and PET/MR in the diagnosis of Parkinson’s disease，focusing on their potential to enhance diagnostic accuracy and facilitate early detection.

Abstract:Obesity is caused by a long-term excess of energy intake over expenditure which leads to the storage of excess energy as fat in adipose tissue or other tissues. The activation of adipose tissue dependent adaptive thermogenesis could increase energy expenditure and improve glucose and lipid metabolism，which is promising target for obesity. Both brown adipocytes and beige adipocytes participate in thermogenesis. Thermogenic adipocytes in adults are more close to beige adipocytes which have the capability to switch between an energy storage and energy dissipation phenotype，and could be activated by cold or adrenegic stimuli. Mitochondrial protein uncoupling protein 1 in thermogenic adipocytes could dissipate energy as heat. Recent studies have found that mitochondrial dynamics，quality control，metabolites and itself as the signaling organelles play vital roles in regulating beige biogenesis and function. This review will discuss the characteristics of mitochondrial and how mitochondrial regulate beige adipose tissue.

Abstract: