Abstract:Objective：Due to inconsistent subjective assessment criteria and excessively long calculation responses，there is a high risk of mistakenly discarding suspected hemolytic plasma and inappropriately discarding suspected non - hemolytic plasma during plasma preparation，posing significant risks to patient safety and leading to waste. This study aims to resolue these problems. Methods： A thresholding method that integrates deep learning with color-changing ink concepts was developed. By employing an enhanced U-Net architecture for image segmentation，the study introduces an advanced attention mechanism，batch normalization，and a padding module to tackle issues such as mean estimation bias，computational inefficiencies，and limited receptive field sizes in spatial mapping relationships. The model was validated and compared using a self-collected sample dataset. Results：This study employed the color-changing ink boundary method for classification，enhancing the computational efficiency of plasma discrimination and reducing discrimination time，while ensuring the accuracy of plasma sample identification. The accuracy rate of the experimental results is 99.52%. Conclusion：The results indicate that the plasma discrimination accuracy of this model is superior to other discrimination models，and it is expected to be applied in clinical practice.

Abstract:Objective：To construct a recombinant adenovirus（Ad-DF3-copGFP）containing the DF3/MUC1 promoter transcriptional regulatory sequence and green fluorescent protein（GFP）and to investigate its role in the detection of circulating tumor cells（CTC）. Methods：A recombinant adenovirus（Ad-DF3-copGFP）was prepared and purified. Comparative studies were conducted with a previously constructed and stored recombinant adenovirus（Ad-hTERT-copGFP）in our laboratory. Infection efficiency and non-specific infection rates were evaluated by infecting lung adenocarcinoma A549 and H1299 cells，as well as peripheral blood mononuclear cells （PBMC）from healthy individuals. A549 cells were artificially added to healthy peripheral blood to simulate CTC，and the infection with both recombinant viruses was used to detect simulate CTC for detection rate determination. The Ad-DF3-copGFP and Ad-hTERT-copGFP methods were used to detect CTC in clinical samples from lung cancer patients，and the initial clinical CTC detection performance was evaluated. Results：The recombinant adenovirus（Ad -DF3-copGFP）was successfully constructed，showing a high infection efficiency for both A549 and H1299 cells. Compared with Ad-hTERT-copGFP，Ad-DF3-copGFP showed a lower non-specific infection rate（P ＜ 0.001）. The overall detection rate using the Ad-DF3-copGFP method（77.3%）was higher than that using the Ad-hTERT-copGFP method（69.6%）. In clinical CTC detection，the number of CTC detected by the Ad-DF3-copGFP method[（10.90± 2.42）cells per 4 mL]was significantly higher than that by the Ad-hTERT-copGFP method[（6.20±1.81）cells per 4 mL，P ＜ 0.001]. Conclusion：The recombinant adenovirus（Ad-DF3-copGFP）is successfully constructed，demonstrating a reliable and efficient detection of CTC，thus providing a new method for CTC detection.

Abstract:Objective：To explore the role of sublytic C5b -9 upregulating the transcription factor Krüppel -like factor 5（KLF5）in promoting the production of the inflammatory cytokine interleukin-23（IL-23）in glomerular mesangial cells（GMCs）of rats with Thy-1 nephritis（Thy-1N）. Methods：①A rat model of Thy-1N was established and rat GMCs were cultured in vitro. The expression levels of KLF5 and IL-23 in the renal tissues of Thy-1N rats and in GMCs stimulated by sublytic C5b-9 were detected by using Western blot （WB）. ②The levels of mRNA and protein of KLF5 and IL -23 in the GMCs transfected with either a KLF5 overexpressing plasmid （pIRES2 - KLF5）or a KLF5 small interfering plasmid（shKLF5）were examined by RT - qPCR and WB. ③The full - length IL - 23 promoter luciferase reporter gene plasmid（pGL3-IL-23-FL）was transfected into GMCs，followed by stimulation with sublytic C5b-9.Alternatively，pGL3- IL -23-FL was co -transfected with either pIRES2-KLF5 or shKLF5 into GMCs，and changes in IL -23 promoter activity were measured using a luciferase reporter gene assay. ④The LV-shKLF5 and LV-shCTR lentivirus vectors were respectively perfused into rat renal tissues via the artery perfusion. After confirming that LV - shCTR could enrich in rat kidney through animal imaging system and frozen section，the Thy-1N was reproduced，and the KLF5 and IL-23 expression in the renal tissues were measured by WB. Results：①The expressions of KLF5 and IL -23 were significantly increased in the renal tissues of Thy -1N rats and in GMCs stimulated by sublytic C5b-9，with KLF5 expression peaking slightly earlier than IL-23. ②Overexpression or knockdown of KLF5 in GMCs led to an increase or decrease in IL - 23 expression，respectively. ③ Sublytic C5b - 9 stimulation or KLF5 overexpression upregulated the activity of IL-23 promoter，while KLF5 knockdown markedly reduced the IL-23 promoter activity induced by sublytic C5b-9. ④IL-23 expression in the renal tissues of the rats treated by knocking down of renal KLF5 gene was significantly downregulated. Conclusion：In the early stage of Thy-1N in rats，sublytic C5b-9 stimulates GMCs to promote the transcription and expression of the IL-23 gene by upregulating KLF5.

Abstract:Objective：To investigate the dynamic changes of serum carbohydrate antigen 125（CA125）and human epididymal protein 4（HE4）levels before and after surgery and during chemotherapy，and their relationship with progression-free survival（PFS） and platinum sensitivity in patients with ovarian cancer. Methods：A retrospective analysis was conducted on serum CA125 and HE4 levels before surgery，before chemotherapy，and at least twice during chemotherapy in standardized treated patients with ovarian cancer in the gynecologic oncology department of the First People’s Hospital Affiliated with Shanghai Jiao Tong University School of Medicine. Data on platinum drug sensitivity and PFS were collected. The main statistical methods included chi-square test，t-test， logistic regression analysis，and Cox proportional hazards regression analysis. Results：The analysis of 117 patients with high -grade serous ovarian cancer suggested that CA125 and HE4 positivity were associated with higher stage，greater ascites volume， unsatisfactory surgical debulking，and positive ascites cytology. Cox regression analysis verified that both CA125 and HE4 are prognostic risk factors（OR=4.29，P=0.010；OR=1.77，P=0.049）. Patients with both CA125 and HE4 positive and those with only CA125 positive showed no significant difference in prognosis（P ＞ 0.05）. The half-life of CA125 and HE4 were calculated using the formula t_1/2=t₁/ [ 2×lg（c₁/c₂）]. The optimal cut-offs for preoperative and postoperative CA125 and HE4，and the half-life of CA125 and HE4 were determined by X-tile and were 436 U/L，400 pmol/L，12 U/L，35 pmol/L，21 days，and 25 days，respectively. The prognostic analysis showed that except for the lowest value of HE4，all other factors were related to prognosis. The highest HR value was 3.28 for CA125 half -life ＞21 days，with a decrease of 57.5% in median PFS（P ＜ 0.001）. The area under the curve（AUC）was calculated by plotting the receiver operating characteristic（ROC）curve. ROC curve analysis showed that CA125 half-life ＞21 days （AUC=0.76），CA125 lowest value ＞12 U/L（AUC=0.70），and failure of CA125 to return to normal after the third cycle of chemotherapy（AUC=0.71）have certain clinical value for predicting recurrence within three years，with sensitivities of 71.8%，68.3%， and 68.2%，and specificities of 79.6%，71.7%，and 72.6%，respectively. Conclusion：CA125 half -life，the lowest value of CA125 during chemotherapy，CA125 not returning to normal after the third cycle of chemotherapy，and having≥2 positive indicators among the 6 indicators have certain clinical value in predicting recurrence within 3 years. Further exploration is needed to assess their value in predicting platinum sensitivity.

Abstract:Objective：To construct a new model for predicting the outcomes and therapeutic efficacy of colorectal cancer（CRC）. Methods：Firstly，the univariate analysis and the least absolute shrinkage and selection operator（LASSO）-Cox regression analysis were used to train the GSE39582 dataset to construct a prognostic signature of colorectal cancer（PSCRC），and external datasets CRC_TCGA and GSE17536 were used to validate PSCRC. The correlations of PSCRC with clinical indicators，tumor immune microenvironment and immune cells infiltration were evaluated，and the molecular function of PSCRC was analyzed by gene set enrichment analysis（GSEA）. Next，seven factors，such as PSCRC and clinical stage，were integrated to draw a prognostic nomogram， and the prognostic effect was evaluated by the decision curve analysis（DCA）. Finally，the efficacy of immunotherapy and chemotherapy was predicted. Results：We constructed a PSCRC，which was validated by two external datasets，confirming its high prognostic sensitivity and specificity. TNM staging significantly affected the risk scores of PSCRC（all P ＜ 0.001）；PSCRC showed significantly positively correlations with tumor microenvironment matrix（TME）score，immune score，and ESTIMATE score（all P ＜ 0.001），significantly positive correlations with infiltrations such as neutrophils（all P ＜ 0.05），and significantly negative correlations with infiltrations like activated memory CD4 ⁺ T cells（all P ＜ 0.01）. In addition，the GSEA analysis indicated that PSCRC might participate in oxidative phosphorylation，angiogenesis，hypoxia and inflammatory response. Importantly，the newly constructed model showed a good prognostic ability，with a C-index of 0.765 and a 95% confidence interval（CI）of 0.747 to 0.783（P ＜ 0.001）. Finally，in the three datasets，the therapy prediction results revealed that the low-risk scoring group had a high response rate to immunotherapy （all P ＜ 0.001）；PSCRC was significantly negatively correlated with the half inhibitory concentration（IC₅₀）of chemotherapy drugs such as imatinib，dasatinib，and pazopanib（all P ＜ 0.001），and significantly positively correlated with the IC₅₀ of metformin，sorafenib and other drugs（all P ＜0.001）. Conclusion：We construct a PSCRC，which provides a robust model for CRC prognosis. and also offers a potential marker for predicting treatment.

Abstract:Objective：To investigate the diagnostic value of balloon-assisted small enteroscopy and other examination methods for Meckel’s diverticulum of the small intestine. Methods：A retrospective analysis was conducted on 87 patients diagnosed with Meckel’s diverticulum at the First Affiliated Hospital of Nanjing Medical University from January 2010 to December 2023. Data regarding demographics，clinical presentations，diagnostic methods，treatments，and pathological findings were collected. Clinical characteristics were summerized，and the diagnostic efficacy of balloon-assisted small enteroscopy was compared with other examination methods for detecting Meckel’s diverticulum. Results：A total of 87 patients with Meckel’s diverticulum were included，with 55 patients had complications such as gastrointestinal bleeding，intestinal obstruction，diverticulitis，and intestinal perforation. Compared to patients without complications，those with complications had a younger median age（P=0.003），longer diverticulum diameter[（5.2 ± 2.6）cm vs. （3.3 ± 1.4）cm，P=0.001]，wider opening[（2.4 ± 1.1）cm vs.（ 1.6 ± 0.7）cm，P=0.002]，and higher surgery rate[（89.1% vs. 56.3%，P ＜ 0.001]. The diagnostic sensitivity of different examination methods for Meckel’s diverticulum were as follows：balloon-assisted small enteroscopy 94.4%，nuclide imaging（ectopic gastric mucosal imaging）58.3%，capsule endoscopy 30.0%，small intestine CT 9.4%， abdominal CT 8.2%，and digital subtraction angiography 0%. Conclusion：Young patients with longer diverticulum diameter and wider opening of small intestine Meckel’s diverticulumin are more prone to complications such as diverticulitis，perforation，bleeding，and obstruction. Balloon-assisted small enteroscopy has the highest preoperative diagnostic value for Meckel’s diverticulum and should be the preferred examination method for patients suspected of having small intestine Meckel’s diverticulum.

Abstract:Objective：To explore the factors influencing lateral episiotomy in vaginal deliveries of suspected macrosomia. Methods： The clinical data of 149 pregnant women with suspected macrosomia who delivered at the Obstetrics Department of the First Affiliated Hospital of Nanjing Medical University from January to July 2022 were analyzed retrospectively. According to whether the perineum was cut laterally during vaginal delivery，the perineum was divided into two groups：the episiotomy group and the natural laceration group. Risk factors for vaginal delivery in pregnant women with suspected macrosomia were analyzed. Results：Among 149 pregnant women with suspected macrosomia，lateral episiotomy was performed in 40 cases（26.85%），while 109 cases（73.15%）had vaginal deliveries accompanied by natural laceration，including 93 cases of Grade Ⅰ laceration and 16 cases of Grade Ⅱ laceration. The proportion of primipara and midwives with less than 5 years of experience was higher in the episiotomy group compared to the laceration group（P ＜ 0.001，P=0.021）；the proportion of the second stage of labor less than 47 min in the episiotomy group was significantly higher than that in the natural laceration group（87.5% vs. 39.4%，P ＜ 0.001）. In addition，the proportion of amniotic fluid properties（clear +Ⅰ degree）in the natural laceration group was significantly higher than that in the episiotomy group（87.2% vs. 72.5%，P=0.034），while the rate of postpartum hemorrhage was significantly lower than that in natural laceration group（P=0.010）. Primipara and midwives with less than 5 years of experience were independent risk factors for episiotomy in suspected macrosomia （OR=2.708，P=0.021）. Conclusion：The incidence of lateral episiotomy in vaginal delivery of suspected macrosomia increases in primipara and midwives with less than 5 years of experience，at the same time，the rate of postpartum hemorrhage in the lateral episiotomy group is significantly reduced.

Abstract:Objective：To investigate the association between metabolic syndrome（MS）and its components with the risk of stroke incidence. Methods：A prospective cohort study was conducted to compare the differences in demographic information，behavioral lifestyle，dietary habits，medical history，and MS - related indicators between people with and without stroke. The Cox proportional hazards regression analysis model was used to analyze the association between MS components，their interactions，and the incidence of stroke. Results：Stroke patients had higher age，waist circumference（WC），body mass index（BMI），blood pressure（BP），total cholesterol（TC），triglycerides（TG），and fasting plasma glucose（FPG）compared to non - stroke patients. After adjusting for confounding factors，MS，WC，TG，BP，and FPG were all associated with an increased risk of stroke，with abnormal BP showing the strongest association with stroke incidence. There were no multiplicative interaction or additive interactions between WC，TG，BP，and FPG. Conclusion：MS is associated with an increased risk of stroke incidence. WC，TG，BP，and FPG independently contribute to stroke risk，with abnormal BP exerting the greatest impact on stroke incidence.

Abstract:Objective：To explore the curative effect and clinical applicability of microsurgical repair of spinal extradural meningeal cysts（SEMC）fistulas combined with cyst tail end technique in the thoracolumbar spine. Methods：Data from 12 patients who underwent thoracolumbar SEMC fistula repair combined with the cyst tail end technique（removal of the cystic sac at the tail end of the cyst，followed by a small incision at the junction of the cyst tail end and the normal dura mater to connect with cerebrospinal fluid，and then routine suturing）under electrophysiological monitoring microsurgery were retrospectively reviewed，and analyzed in conjunction with traditional surgical methods from the literature. Results：During an average follow-up of 14 months，complete cyst disappearance was observed in 9 cases，partial disappearance in 1 case，and residual cysts in the intervertebral foramen in 2 cases. No cases exhibited dural sac compression，and there were no recurrences. Among the 11 patients who had preoperative lower back pain and/or lower extremity pain，pain was relieved or disappeared upon discharge，and no significant differences in pain were observed 3 months of follow -up compared to discharge. Paitents with lower limb weakness and dysuria showed gradual recovery，and no new neurological deficits were noted postoperatively. The anatomical reduction of lamina spinous processes was good，with no cases of spinal stenosis and deformity. Conclusion：Electrophysiological monitoring microsurgical thoracolumbar SEMC fistula repair combined with cyst tail end technique effectively solve the problem of living flap and close cyst，with no significant complications，demonstrating clinical feasibility.

Abstract:Objective：To assess the value of sampling perfection with application optimized contrast using different flip angle evolution（SPACE）sequence combined with compressed sensing（CS）technique in the diagnosis of acromioclavicular joint injury. Methods：Total 34 patients with a history of shoulder trauma and suspected acromioclavicular joint injury in the First Affiliated Hospital of Nanjing Medical University were prospectively included form May，2023 to February，2024. Conventional 2D magnetic resonance sequence and 3D CS-SPACE sequence scanning based on CS were performed on the patients. Signal intensity and standard deviation of long head tendon of biceps and bone marrow cavity of humerus were measured on two sets of images，and SNR and CNR were calculated. Three doctors assessed the injuries of the acromioclavicular joint through two sets of images and gave their diagnostic confidence rating. The SNR，CNR of bone marrow cavity and biceps long tendon，and diagnostic confidence ratings were compared between the two groups. The diagnostic consistency of the three doctors in conventional 2D images and 3D CS - SPACE images was analyzed respectively，and the diagnostic consistency between the two sets of images was evaluated. Results：In the objective evaluation of image quality，SNR and CNR of 3D CS-SPACE image were obviously better than that of conventional 2D image. For the two groups of images，two doctors’ratings of 3D CS-SPACE images were significantly higher than those of conventional 2D images，and one doctor’s ratings had no significant difference. The three doctors demonstrated a strong agreement in the evaluation of acromioclavicular joint injury on both conventional 2D images and 3D CS-SPACE images（κ＞0.6），and there was a high consistency in the evaluation of acromioclavicular joint injury between the two groups of images（κ ＞0.6）. Conclusion：For the diagnosis of acromioclavicular joint injury，3D CS-SPACE sequence images has high consistency with conventional 2D images，and 3D CS-SPACE sequence can shorten the scanning time and obtain better image quality.

Abstract:Objective：To observe the features of brain regional homogeneity（ReHo）in children with different degrees of obesity aged 7-15 years by resting state functional magnetic resonance imaging（rs-fMRI）. Methods：Resting-state fMRI data were collected from 35 cases obesity children（OB），20 cases obesity children with metabolic syndrome（MS），and 24 cases healthy children（HC）. The data were analyzed，and ReHo differences among the three groups were compared. Then the correlation between the different brain regions and clinical data was analyzed. Results：Compared with HC group，the ReHo values of right fusiform gyrus and left inferior temporal gyrus in OB group and MS group were higher. The ReHo values of right medial superior frontal gyrus and left dorsolateral superior frontal gyrus decreased. Compared with OB group，the ReHo value of left dorsolateral superior frontal gyrus in MS group was lower. The ReHo values of left dorsolateral superior frontal gyrus were negatively correlated with alanine aminotransferase（ALT）and aspartate aminotransferase（AST）（r=-0.350，P=0.009；r=-0.294，P=0.029）. Conclusion：Obese children have abnormal functional activity in brain areas such as participating in feeding regulation and inhibitory control，and the abnormal activity in the left dorsolateral superior frontal gyrus is associated with the degree of obesity，which may provide imaging evidence for early intervention and treatment.

Abstract:Objective：To explore the correlation between the fat attenuation index（FAI）and plaque quantification parameters with brain white matter hyperintensities（WMH）. Methods：A retrospective collection of clinical，coronary computed tomography angiography（CCTA）and brain magnetic resonance imaging（MRI）data of 407 patients from January 2021 and December 2022 was conducted. The severity of WMH was assessed using the Fazekas score，dividing patients into mild WMH group（Fazekas score 0-2） and moderate to severe WMH group（Fazekas score 3-6）. Clinical data，FAI，and plaque quantification parameters of the two groups were compared to identify independent risk factors for moderate to severe WMH. Results：There were statistically significant differences in FAI and plaque quantification parameters between the two groups（P ＜ 0.05）. The results of multivariable logistic regression showed that age（OR=1.116，95%CI：1.074-1.160，P ＜ 0.001），coronary artery disease（OR=2.737，95%CI：1.270-6.017，P= 0.010），plaque burden（OR=4.142，95%CI：1.228-13.977，P=0.022），and FAI ＞-70.1 HU（OR=5.089，95%CI：1.899-13.640，P= 0.001）were independent influencing factors for moderate to severe WMH. Conclusion：Older age，presence of coronary artery disease， higher plaque burden，and FAI＞-70.1 HU are associated with a higher likelihood of developing moderate to severe WMH，providing a theoretical basis for the joint management of coronary atherosclerosis and WMH.

Abstract:Objective：To explore the correlation and predictive value of shear wave elastography（SWE）of the rectus femoris with postoperative pulmonary complication（PPC）following thoracoscopic surgery. Methods：Clinical data from 292 patients scheduled for elective thoracoscopic surgery were collected. Patients were divided into the PPC group and the non-PPC group，based on the ocurrence of PPC postoperatively. Preoperative assessments of the rectus femoris included conventional ultrasound combined with SWE， measuring rectus femoris thickness（RF_thick），cross-sectional area（RF_csa），and mean share wave velocity（CS_mean）. Differences in clinical data and the rectus femoris ultrasound parameters between the two groups were compared. The correlation with PPC occurrence was analyzed，and a combined diagnostic formula was derived using binary logistic regression analysis. ROC curves were plotted to further analyze the predictive value of single and combined indicators for PPC. Results：The occurrence of PPCs was positively correlated with age and negatively correlated with the rectus femoris CS_mean（P ＜ 0.001）. Age and CS_mean alone had lower predictive efficacy for PPCs， while their combination showed higher efficacy，with the area under cure of 0.714. Conclusion：Analysis of the rectus femoris CS_mean is feasible for predicting the occurrence of PPCs，and its predictive value is enhanced when combined with age. This non -invasive and rapid assessment can evaluate the risk of PPC in patients undergoing thoracoscopic surgery.

Abstract:Objective：To explore the predictive value of high definition flow imaging（HD-Flow）in detecting different systolic parameters of M1 segment of the fetal middle cerebral artery（MCA）for fetal distress（FD）in preeclampsia（PE）. Methods：A total of 120 PE patients who underwent HD -Flow detection in the First Hospital of Hebei Medical University from June 2019 to June 2023 were selected as the study objects. According to the occurrence of FD，the patients were divided into the non -occurrence FD（NFD） group（n=64）and the FD group（n=56）. The clinical data and systolic parameters of distal M1 segment and proximal 1/3 of M1 segment were compared between the two groups. LASSO logistic regression analysis model screened the potential factors of FD，and multivariate logistic regression analyzed the risk factors affecting the occurrence of FD. The risk prediction model of FD occurrence was constructed and verified. The predictive ability of each index to FD occurrence was analyzed. The receiver operating characteristic（ROC）curve was plotted and the area under the curve（AUC）was calculated to analyze the predictive value of different systolic parameters for the occurrence of FD. Results：LASSO regression analysis screened out 15 predictors，and logistic analysis results showed that gestational age，intrauterine infection，gestational diabetes mellitus，partial pressure of carbon dioxide（PCO₂），resistance index（RI），pulsatility index（PI），peak systolic velocity（PSV），and peak systolic velocity/end diastolic velocity（S/D）at the distal and mesial 1/3 of M1 segment were independent risk factors for FD. The AUC before and after ROC curve verification of the constructed prediction model were 0.801（95%CI：0.696-0.845）and 0.785（95%CI：0.688-0.829），respectively，indicating a good model differentiation and a high accuracy. Gestational age，intrauterine infection，gestational diabetes mellitus and PCO₂ indexes were added to the systolic parameters at the distal and mesial 1/3 of M1 segment in MCA，and the prediction ability was the best. Systolic parameters in different segments of M1 had different predictive values，in which the AUC of distal RI，mesial 1/3 RI and S/D were 0.926（95%CI：0.992-0.826），0.904 （95%CI：0.979-0.785）and 0.917（95%CI：0.984-0.807），respectively. Conclusion：Systolic parameters of different M1 segments in fetal MCA way predict the occurrence of FD to a certain extent. RI at the distal part of M1 segment，as well as RI and SD at mesial 1/3 of M1 segment in MCA have a high predictive performance. The establishment of a risk prediction model for FD way be used to accurately predict the risk of FD in PE patients and provide targeted intervention for FD.

Abstract:Long non-coding RNA（lncRNA）is a class of non-coding RNA molecules with a length of more than 200nt. Numerous studies have reported their significant roles in the occurrence and development of malignant tumors. In recent years，nultiple studies have highlighted the high expression of small nucleolar RNA host gene 20（SNHG20），a type of lncRNA，in various carcinomas such as lung cancer，liver cancer，and gastric cancer. SNHG20 has been shown to promote tumor cell proliferation，migration，invasion and epithelial-mesenchymal transition（EMT），inhibit apoptosis，affect cell cycle progression，and correlate with poor prognosis in patients. As a recognized oncogene，lncRNA SNHG20 is a potential therapeutic target and prognostic biomarker in malignant tumors. In this article，we summarize relevant research reports around the world，and review the research progress of lncRNA SNHG20 in malignant tumors.

Abstract:Some degree of localized hypoxia can cause varying degrees of cellular damage in tissues. Oxygen-releasing biomaterials can sustainably provide oxygen to transplanted cells. In recent years，extensive research efforts have been dedicated to developing more pratical oxygen-releasing biomaterials and systems to enhance oxygen delivery in vivo and ensure effective oxygen supply to implanted tissues. Based on the form of oxygen provision，these materials can be calssified into oxygen-carrying materials and oxygen-producing materials. The former deliver therapeutic oxygenation by binding oxygen molecules under specific conditions，while the latter incorporate oxygen source materials into polymers，achieving oxygen release through in vivo hydrolysis. This paper summarizes the current development of oxygen-carrying materials and oxygen-producing materials，including oxygen source supply，loading materials， and controlled release. It discusses practical application of oxygen-releasing biomaterials for oxygen delivery and various strategies for controlled release.

Abstract:Epilepsy is a kind of brain dysfunction caused by abnormal synchronous discharge of brain neurons，and is one of the common diseases of the nervous system. Patients need to take anti-epileptic drugs regularly for a long time to achieve seizures-free. For pregnant women，antiepileptic drugs can enter the fetus through the placental barrier causing adverse effects. Lamotrigine and levetiracetam have the lowest potential teratogenic risk，oxcarbazepine has a low risk，topiramate has high risk，while valproic acid has the most severe teratogenic effect and negative effects on the neuropsychological development of children exposed in utero，and should be avoided as far as possible. At present，the management of antiepileptic drugs is still a difficult clinical problem. Pre-pregnancy preparation and rational decision-making of antiepileptic drugs usage during pregnancy are very important.

Abstract:Objective：As the most common metabolic disease，diabetes is closely related to cardiovascular disease and is an independent risk factor for the occurrence of acute myocardial infarction. Nuclear factor erythroid-2-related factor 2（NRF2）is a key regulator of the cellular antioxidant response，which increases the transcription of downstream antioxidant genes by interacting with other proteins and binding to antioxidant response elements，thereby maintaining the dynamic balance of redox states. Some studies have shown that NRF2 is involved in the regulation of the occurrence and development of diabetic myocardial ischemia - reperfusion injury，but its mechanism is not yet fully understood. This paper mainly reviews the research progress of NRF2 signaling pathway related to diabetic myocardial ischemia - reperfusion injury and NRF2 targeted therapies，providing new insights for the clinical treatment of diabetic myocardial ischemia-reperfusion injury.

Abstract:With the widespread use of plastic products globally，plastic pollution is becoming more and more serious. Plastic particles formed by the breakdown of plastics have received significant attention in recent years. There are various sources of plastic particles. Plastic particles can enter the human body through respiratory tract inhalation，digestive tract intake，direct skin contact and other ways，and then affect multiple systems of the human body through oxidative stress，inflammatory response and other mechanisms. In addition，plastic particles can also be co -exposed with additives，heavy metals，bacteria，viruses and other toxic pollutants，which have an impact on the human body. This review summarizes the definition and sources of plastic particles，their entry into the human body，detection methods and their effects on various human systems. It serves as a theoretical basis for subsequent efforts in preventing and mitigating plastic pollution and reducing the harmful impact of plastic particles on human health.

Abstract: