To understand the difference in the perceptions of ethical issues in the medical application of artificial intelligence(AI)among medical personnel in public hospitals as well as its influencing factors and provide suggestions for improving the medical personnel’s ethical cognition and ethical decision-making ability in the application of artificial intelligence in the medical field in public hospitals，a total of 813 medical personnel participated with a self - designed questionnaire by convenient sampling method in a tertiary public hospital in Anhui Province. The channels for medical personnel to understand the ethical problems in the medical application of AI were diversified，mainly through news and popular scientific articles. Medical personnel believed that security risk and privacy protection were the most critical ethical issues in the medical applications of AI， accounting for a high proportion of 76.1% and 64.9%，respectively. Their length of medical service，professional titles，and knowledge of AI medical applications were related to their perception of ethical problems in AI medical application(P＜ 0.05). Most medical personnel have a low level of understanding of the application of AI in medicine and its ethical problems，with a lack of systematic cognition. We suggest strengthening the education and training of medical personnel on the ethical knowledge in AI medical application；taking effective measures to improve the ethical awareness and the ability of medical personnel in the medical application of AI； standardizing the development of the algorithm of medical AI technology；improving the ethical supervision system of medical AI，and promoting relevant legislation to promote the reliable development of medical artificial intelligence.