Taking 592 nurses from the tertiary hospitals in the Anhui Province as the survey objects，this paper explored the effect of organizational climate perception on nurses’job satisfaction and the mediating effect of job burnout through a questionnaire survey. The results showed that the job burnout of nurses in the tertiary hospitals in Anhui Province was more prominent among young and unmarried nurses，with relatively low job satisfaction. Nurses with longer working life，high professional titles，and formal establishment have higher job satisfaction. The better the perceived organizational climate leads to lower incidence of job burnout and higher the job satisfaction. Job burnout partially mediates organizational climate perception and job satisfaction， accounting for 46.13%. The results of this study suggest that we should pay attention to the physical and mental health as well as job satisfaction of nurses；creating a good workplace climate of trust，communication，emotion and fairness，while offering nurses a positive working environment with organizational support to alleviate their job burnout，improve their job satisfaction，and stabilize and develop the nursing team.