A self-designed questionnaire was used anonymously to investigate the knowledge and awareness level of human papilloma viruses（HPV）and vaccination behavior among interns from four hospitals in Jiangsu Province. This study aims to explore the influencing factors of vaccination behavior. A total of 1 250 questionnaires were distributed，and 1 230 valid copies were recalled. The results showed that the awareness rate of cervical cancer was 53.6%，while that of HPV was 33.3% and 54.7% of the students were aware of HPV vaccination. The knowledge of vaccine was obtained through school education（42.0%），official propaganda （36.1%），media communication（35.2%），and interpersonal communication（30.2%）. More than 60% of the respondents believed vaccine’s safety，efficacy，and immunity duration would affect its promotion，and 54.3% of them could accept vaccines at a cost less than 500 yuan. The vaccination rate of sampled female students was 15.7%. Multi -factor logistic regression analysis showed that the higher acceptable cost of the vaccine，getting the knowledge of the vaccine through interpersonal communication，the belief that HPV can be reinfected after recovery，and education level were important factors in promoting the vaccination behavior. Learning about the vaccine through media，such as television networks，negatively impacts vaccination behavior. Therefore，it is necessary to adopt comprehensive measures to improve the vaccination rate and emphasize interpersonal communication as a means of information dissemination.