To understand the meaning in life of medical students and its influencing factors，631 college students in a medical college in Jiangsu Province were selected to conduct an online survey，providing a reference for formulating targeted intervention measures. The results showed that the total score of medical students’meaning in life（101.04 ± 18.65） was in the middle level. The total score and the scores of each dimension of the psychological capital of the surveyed medical students are positively correlated with the total score and the scores of each dimension of their sense of meaning in life. Regression analysis showed that gender，whether holding a leadership position，family rearing style，and psychological capital were the influencing factors of medical students’sense of meaning in life. The study concludes that medical students’ sense of meaning in life needs to be improved and college educators may formulate corresponding educational strategies based on different influencing factors.