Objective: To explore the stability of undergraduate students’ personality deviation across time and its relationship with personality traits. Methods: The basic information scale, Personality Diagnostic Questionnaire-4 and Chinese big five personality inventory were used twice among sophomores in the four universities. At last 179 students were been tracked. Results: Repeat analysis of variance indicated that except the antisocial personality deviation was statistically significant ((F=7.055,P＜0.01)) across time, the rest dimensions of personality deviation showed no statistically significant. Schzotypal, antisocial, borderline and narcissistic personality deviations were statistically significant of gender main effect (F =7.682,16.641,7.060,21.420,respectively；P＜0.01, all). Regression analysis suggested that neuroticism trait was extremely significant positive correlation with personality deviation (t = 6.092, P <0.01), and agreeableness was significant negative correlation with it (t = -2.169, P <0.05). Conclusion: The dimensions of personality deviation were stable among the university students. Neuroticism and agreeableness traits related to personality deviation.