Objective：To know the present allocation and forecast the reasonable requirements of public health human resources in township health centers. Methods：The total man-hour of public health services and per capita man-hour could be calculated base on the inquiry of all the 152 public health manpower working in 21 township health centers in Gaoyou city of Jiangsu province with a self-made questionnaire. Results：The structure of public health manpower is unreasonable and the number of public health human resources is deficient in township health centers. We forecasted the number of professional public health manpower should be 170.53 to complete all public health service, which corresponded to 2.71 per 10,000 people by needs-based approach. Conclusion：It is expected to increase the number of public health human resources, strengthen the training of them and optimize the structure of public health manpower. The predicted results are reasonable and credible.