Objective: To explore the self-forgiveness status and its related factor of medical students and the correlation with the variable of mental health, and provide an evidence to improve the self-forgiveness and the mental health. Methods: 720 medical students of 3 medical university were randomly selected as the research sample, use the Self-made questionnair and some Scales.Results: Self-forgiveness level of medical students is 27.0±4.74, medical students’ self-forgiveness level have no statistically significant in grade and specialty(P＞0.05), some factors of guilt、anger、personality related to self-forgiveness significantly(P＜0.05), and self-forgiveness of medical students was significantly related to anxiety、depressed and happiness(P＜0.05). Conclusion: Guilt、anger、personality of medical students have different effects on self-forgiveness, their self-forgiveness level also affects their mental health and well-being.