Objective：To realize the status of junior college students’ health literacy in Nanjing Medical University. Methods：A questionnaire survey was conducted to investigate 197 medical college students by using the multistage cluster sampling method. Results：The proportion of students who had held health literacy was 16.24%.The holding rate of health knowledge and concept, the forming rate of health behavior and the mastering rate of health skill were 45.17%, 5.58% and 55.84% respectively. The holding rate of health literacy among male students was higher than that of female students. The freshman had higher health literacy than sophomore students. The holding rate of health literacy among students from urban areas was higher than that of students from rural areas. Parental educational level and monthly living expenses were relate to health literacy level. Conclusion：The forming rate of health behavior was on the low side. Carry out targeted health education for different groups of college students according to the survey results. Strengthen the propaganda and education of the relevant knowledge of the correct rate of the answer in the questionnaire. Improve the work of school health education in multi-ways.