Objective: To part of the tertiary general hospital in jiangsu provinceas an example, analysis of the expression condition of medical professional attitude, provide the basis for doctors to improve the professional manner, and put forward feasible suggestions. Methods: The self-designed questionnaire to both doctors in jiangsu province eight level 3 general hospital were investigated, and the survey data from descriptive analysis and chi-square test. Results: (1) 54.0% of the doctors think expression can pass medical humanities, namely nearly half of the doctors lack of the cognition; (2) 65.3% of the doctors have been exposed the micro expressions which is not accepted by the patients; (3) Patients with the highest recognition of the doctors’s demeanor is kind and focus; (4) The main factors influencing the doctor look there is work stress. Conclusion: Doctors lack of the cognition of medical professional attitudes of expression, the perception of it is not comprehensive; Doctors need to improve the cognition to the expression, to strengthen the control of expression, improve the effectiveness of doctor-patient communication, promote the construction of a harmonious doctor-patient relationship. But also lighten the doctors working pressure, enhance the doctor work enthusiasm, improve the professional manner of a doctor. Performance with a look of the doctor is beneficial to develop the quality medical service quality of the hospital, is conducive to the establishment of humanistic hospital good image.