Micro-lecture is a new teaching resource, with the characteristics of short and exquisite. In this paper, the author participated in the National Universities Micro-Teaching Competition and take the micro-lecture as the research object. Then we applied the micro-lecture in the teaching basic medicine subjects and analyzed the learning effect of the students via the questionnaire. The results indicated that the students' understanding of the micro-lecture is limited, but they showed interested in the micro-lecture. And the micro-lecture help them review and expand the capability of scientific thinking. Therefore, the rational use of micro-lecture can provide a convenient and practical research resources for medical students.