Objective: To investigate and analyze the current status of antimicrobial stewardship in tertiary hospitals, compare the results with the implementation of AMS in the international, find out the problems, and propose suggestions. Methods: 50 tertiary hospitals in Jiangsu Province were investigated by convenient sampling and snowball sampling. Results: The number of hospitals that established the professional team for antimicrobial stewardship in 2011 was the largest; 52% of hospitals allocated special funds to support antimicrobial stewardship activities; The most hospitals did not conduct regular education to patients; Only 52% of hospitals regularly gave feedback on antimicrobial use and communicated to physicians; 46% of hospitals did not conduct pre-audit and feedback on antimicrobial prescriptions in the outpatient department, and 22% in wards; 84% of computer systems used antimicrobial stewardship-related software; The administrative interventions were widely used. Conclusion: Improve the multidisciplinary cooperative antimicrobial stewardship team; Set up special funds for antimicrobial stewardship activities; Strengthen education on the use of antimicrobial for patients; Pay attention to feedback on monitoring information; Develop some applications to assist in the management of antimicrobial stewardship; Conduct actively pre-audit and feedback; Change management ideas, and gradually weaken administrative intervention.