With the rapid development of minimally invasive surgery, laparoscopic training was paid more and more attention recently. This study is to establish and evaluate both teaching contents and testing standard of laparoscopic simulation training among medical undergraduates. In this study, 150 medical undergraduates in Grade 2015 participated in laparoscopic basic skill straining and testing. The basic skills of trainees were significantly improved after training. We concluded that early laparoscopic simulation training can improve the basic skills of medical undergraduates. The teaching contents and testing standard deserve a further research and expand.