Human resources are strategic resources to realize national rejuvenation and win initiative of international competition. Since the Third Plenary Session of the Eleventh Central Committee, the CPC Central Committee has attached great importance to personnel cultivation and development, and has repeatedly put forward and deepened “the Strategy of Talented Persons Powering the Nation” and the principle of managing talents by the Party. With the guidance and support of policies at the national and Shanghai levels, ideas that talent is the first resource has been deeply implemented in the important work of CPC building in the health field. Faced with problems of total stability, training system and job acquisition in personnel training of Disease Prevention and Control Institutions (CDC institutions) in the new period, we put forward the main idea of training strategy of CDC institutions in the new era that we adhere to “the Strategy of Talented Persons Powering Health Care” and the principle of managing talents by the Party, grasp the law of growth of public health talents, pay attention to the optimization and management of talent structure, to cultivate the top-ranking CDC institutions and talents serving the health needs of residents and public health services. Thus, we can provide experience and reference for personnel training of CDC institutions in Shanghai and even the whole country.