Objective to investigate the explicit and implicit discriminatory attitudes towards carriers of hepatitis b virus (HBV) and to provide evidence for the prevention and control of HBV. Methods 210 undergraduates from a university in Fuzhou were selected by random sampling to conduct an explicit attitude survey, and 30 undergraduates were randomly selected by using implicit association test to measure implicit attitudes. Results: in terms of explicit attitude, 38.4% of the people had hepatitis b discrimination, which was reflected in the four dimensions of marker (39.2%), stereotype (47.9%), discrimination (40.1%) and discrimination (26.4%), respectively. The D value of implicit attitude test was 0.03, indicating that hepatitis b discrimination also existed. Conclusion hepatitis b discrimination in Chinese society has not completely disappeared. People's most obvious stereotype of hepatitis b virus carriers is poor hygiene habits. Therefore, they differentiate and isolate the carriers in intimate relationship and living space.